• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    In fermentation processes the mass of micro-organisms present, the biomass, is concentrated by coagulation of the solids of the fermentation suspension by the action of an electric current having a frequency of from 0 to 2000 Hz, up to a current density of 10 mA/cm<2>, at a temperature of from 20 to 100 DEG C.
    公开号:SU902673A3
    申请号:SU792746103
    申请日:1979-04-06
    公开日:1982-01-30
    发明作者:Митцлафф Михаэль；Шлингманн Мертен；Фауст Уве
    申请人:Хехст Аг (Фирма)；
    IPC主号:
    专利说明:

    (54) METHOD FOR BIOMASS ISOLATION
    I
    This invention relates to the microbiological industry, in particular to the isolation of microbial biomass from a culture fluid.
    A known method for isolating the biomass of microorganisms from the culture fluid by adding electrolytes SP.
    However, this method is rather time-consuming, since the additives added are undesirable in the subsequent stages of treatment and therefore must be removed from the treatment with high costs.
    The closest in technical essence and the achieved effect is a method of separating the biomass of microorganisms from the culture fluid by treating it with an electric current, in which the diluted suspension is clarified, hardening the particles through a constant voltage electric field of 100-500 V and causing individual particles to move. in this field 2.
    The disadvantages of this method are the incomplete removal of biomass 5 from the culture fluid, as well as the fact that this method is effective only in days of aqueous solutions with a very low solids content.
    The purpose of the invention is a more complete 10 biomass extraction and simplification of the method.
    This goal is achieved by the fact that, in the method of separating the biomass of microorganisms from a culture liquid, by treating it with an electric current, the treatment of the culture liquid with an electric current. com is carried out at a current density of from 0.093 to 9.3 mA / cm and a frequency of 020 2000 Hz at 20-85 ° C.
    In this case, the isolation of microorganisms from the culture fluid is carried out continuously or periodically, preliminarily with its mixing. The essence of the proposed method is that the frequency of the current 0-2000 Hz implies the use of both constant and alternating current. The coagulable particles react with each other when a current is applied, regardless of the electrolytic discharge of gas on the electrodes, and especially in the field of voltage that does not yet produce gas. The suspension is placed in the appropriate reaction apparatus, set the desired temperature and then pass the required current. The amount of electricity required for coagulation depends on the type and concentration of the culture fluid. The proposed biomass can be distributed both periodically and continuously. A reaction apparatus suitable for periodically carrying out the process is, for example, a tank. FIG. 1-3 schematically depicts an apparatus for carrying out the method. In the tank (Fig. 1), the chamber 1 is provided with a tightly closing key 2, through which electricity is supplied for electrodes 3 and 4 and in which there are openings 5 for the inlet of the suspension and 6 for the removal of gases. The gas outlet may be provided with a reflux condenser (not shown) in which the evaporating components of the culture fluid may condense. The tank is jacketed and can be connected to the circulating circuit with a hot or cold liquid through the inlet 7 and the outlet 8 of the nozzle. The temperature of the coolant is monitored with a thermometer 9 or thermocouple. The two electrodes — the anode 3 and the cathode 4 — are spaced from each other at a distance of 0.5-50 mm, preferably 1-15 mm. The materials of the electrodes are, for example, grids or meta.
    palladium or platinum sieves, metal electrodes coated with a noble metal layer, preferably titanium electrodes, oxide layers metal electrodes (as anodes), preferably titanium anodes or also provided with or without slots 9
    which are from 1 mm to 60 mm apart. Accordingly, the tempered culture liquid is fed through the inlet 19 and taken out at the outlet 20. At the same time, along with a single transmission of the culture liquid, it is also possible to transmit it repeatedly. 4 slits plate of graphite. The vertical position of the electrodes can also be replaced by a horizontal arrangement. It is also possible to install several pairs of electrodes that have justified themselves primarily in a block combination of angled or non-curved capillary slot electrodes with or without vibration of the electrodes. During the passage of current, the culture fluid can be stirred, preferably using an agitator, e.g. magnetic stirrer 10, or pumping, especially in block combinations. If the method is carried out continuously, then an additional opening is provided in Krishke 2 of the electrolytic chamber I for continuously transferring the culture liquid. From the culture fluid that is pumped into the circuit, a part of it, if necessary, is taken for further processing and an appropriate amount of fresh culture fluid is added. Another reaction apparatus, suitable in particular for the continuous implementation of the method, is a flow chamber. Particularly suitable are cowers with spade elements (Fig. 2). In the case of a chamber made of plastic or steel with a rectangular section I1 there are two plate electrodes of the simplest design — anode 12 and cathode 13, at a distance from 1 to 60 mm from each other. The culture fluid, suitably tempered, is fed through the inlet 14 and taken from the outlet 15. At the same time, I, together with its transmission, may also be repeated through. Another convenient flow chamber contains tubular elements (Fig. 3). In the housing of a plastic or steel 16 chamber there are two concentric electrodes - anode 17 and cathode 18. Typically, the method is carried out at normal pressure. However, it is possible to work also at higher pressure. Biomass is understood to mean the mass of microorganisms which, during fermentation processes, is contained in the fermentation liquid in the form of solid matter consisting of individual particles. Bacteria, yeast and fungi are commonly used as microorganisms. Examples of such microorganisms are methanol bacteria of the family Methylotope5, for example Methylomonas c) era ATCC 31.266, Candida lipolytica ATCC 20.383, which can be obtained by cultivation on n-paraffin. x in the presence of an aqueous nutrient medium, or mushrooms, which are widely used in the known production of antibiotics, for example, PeniciIium chrysogenum. Example I. Methylonwnas clara ATCC 31.266 is cultivated in a nutrient solution containing methanol as a carbon source, ammonia as a nitrogen source, phosphate, as well as iron salts, magnesium and other common microelements. The cultivation is carried out under azobic conditions. 500 ml of the thus-obtained culture liquid with a solids content. 1.1% by weight is washed down in a tank (Fig. 1) of biomass extraction to the degree of culture. Two concentric platinum grid cylinders with 225 holes / cm with a diameter of 24 and 36 mm and a height of 95 mm are immersed in it. The outer electrode serves as an anode. During electrolysis, the temperature is maintained at 35 ° C. After switching on the direct current, its strength in the first experiment is 0.1 A. This current provides the average current density relative to the anode surface, it is 0.93 mA / cm. After 15 minutes, the current is turned off. The average calculated cell voltage is 1.8 V. Then the contents of the chamber are poured into the sedimentation vessel. The clear supernatant is discarded, and the precipitate is fed for further processing. Experiments 2-5 were conducted in the same way as experiment 1. However, in experiments 3-5, instead of direct current, alternating current was used with frequencies of 20 Hz (experiment 3), 200 Hz (experiment 4) and 2000 Hz (experiment 5) . The corresponding current and voltage values are the effective value values. In tab. I the results of the experiments are compared with the corresponding control experience. Experiments 3-5 show the effective values of current and voltage. Table t is based on processing parameters and
    0.93 0.093 1.67 “0.18 2.79 Control O Note: O) - frequency 20 Hz. c) - frequency 200 Hz, d) - frequency 2000 Hz Ko Neizmer. 50 Neizmer. 45 45 45 Neizmer. 40 5050 45.50 Not measuring Not measured. . ) - effective value, From table. i shows good, very good, sedimentation of samples treated with electric current compared to untreated sample. Example 2. A strain of Candida lipolytica yeast ATCC 20.383 using hydrocarbons is cultured on H-paraffins in an aqueous nutrient medium and oxygen-containing gas. 500 ml of this culture liquid (solids content 2% by weight) is poured into the tank.
    0.093
    0.01
    0.01 0.093
    tr. 0.465
    0.05 0.93
    0.1
    0.93
    0.1
    ABOUT
    About From Table 2, it can be seen that the amount of biomass obtained from samples treated with electric current is significantly higher compared to the untreated sample. Example 3. PeniciIlium chrysogenum ATCC 10.238 is cultured under aerobic conditions in a nutrient medium containing lactopus, liquid Cornsteep, phosphate, carbonate, and magnesium sulfate using the electrodes described in example 1 (test) using electrodes for a definite time, the current is applied. and the effect of the treatment of elec biome
    0.0465
    0,005 0,01 0,093
    9.0
    10.0
    10 15 15 15 30 9.4 0.0 9.6 10.0 9.8 10.0 9.8 10.0 5.0 10.0
    15 15
    1.4
    权利要求:
    Claims (2)
    [1]
    5 30 1.5 (Fig. 1) and treated as described in example 1. The supernatural liquid is drained, the precipitate is dried and weighed. The dry matter weight of the resulting biomass, related to the cell mass contained in the suspension, is a measure of good flocculation. In tab. 2 represents the amount of biomass obtained for five experiments depending on the current strength, voltage, current density and time. Table 2 on the biomass yield measures the filterability of the suspension by measuring the volume of the filtrate with respect to time. Experiment I. 500 ml of mycelial suspension containing 10 wt.% Of a solid substance is electrolyzed with current of 0.005 A at 15 s for 15 min. Then the suspension is filtered on a suction filter (diameter 11 cm, paper, vacuum 15 Torr.) And after 1 min the volume of filtrate is measured. The results of experiments 1-5 of example 3 are summarized in table. 3 and compared with the corresponding control experiment. 3 clearly shows a larger amount of filtrate from samples treated with electrical current as compared to processing the sample. Thus, the proposed method is simpler in comparison with the known, and also allows 50–70% more efficient to separate biomass from the culture liquid. The degree of purity can reach 88% of the initial amount. Claims I. Method for separating microorganism biomass from culture liquid by treating it with electric current, characterized in that, in order to more completely separate biomass and simplify the method, treatment of culture liquid with electric current is carried out at a current density from 0.093 to 9.3 mA / cm and a frequency of 0-2000 Hz at 20-85 C.
    [2]
    2. The method according to claim 1, wherein the microorganisms are inoculated from the culture fluid continuously, periodically, preferably with stirring. Sources of information taken into account during the examination 1. I. Elektrokinetische GrengflSchen vorgange, Verlag Chemie, ein helm, 1977, S. 88. 2 USSR Author's Certificate 523713, cl. B 03 C 5/00, 1972.
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    同族专利:
    公开号 | 公开日
    BE875432A|1979-10-09|
    NO791169L|1979-10-09|
    GB2018287B|1982-07-28|
    AT374494B|1984-04-25|
    CA1128462A|1982-07-27|
    BR7902147A|1979-12-04|
    DD142719A5|1980-07-09|
    ATA252879A|1983-09-15|
    JPS54138186A|1979-10-26|
    NL7902724A|1979-10-09|
    DE2815030A1|1979-10-18|
    FR2421942A1|1979-11-02|
    GB2018287A|1979-10-17|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    FR2513087A1|1981-09-18|1983-03-25|Int Marketing Conseil|Sterilising fluids esp. edible liq. such as fruit juice, milk etc. - by passage between electrodes connected to low voltage pulsed supply|
    FR2516503B1|1981-11-16|1988-03-18|Wehrlen Roland|BACTERIAL ACTIVATION PROCESS IN ORGANIC MATERIAL|
    WO1986006098A1|1985-04-09|1986-10-23|Voest-Alpine Aktiengesellschaft|Process for the simultaneous production of alcohol and protein-rich fodder|
    WO1994008907A1|1992-10-15|1994-04-28|Richter Gedeon Vegyészeti Gyár Rt.|Process for intensification of fermentations|
    JPH0994288A|1995-09-28|1997-04-08|Rimoderingu Touentei One:Kk|Method for inactivating and destructing microbes|
    CN1298501C|2001-05-30|2007-02-07|新日本制铁株式会社|Rail producing method and producing equipment|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    DE19782815030|DE2815030A1|1978-04-07|1978-04-07|PROCESS FOR ENRICHMENT OF BIOMASS|
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