• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    1. DIAGNOSTIC MEANS FOR DETERMINING LEUKOCYTES IN URINE, containing chromogen and buffer solution, characterized in that, in order to increase its specificity, as chromogen, it contains indoxyl or thiondoxyl ester of the general formula 0-A-B where R, jRj, Rj , R is hydrogen, halogen, alkyl with 1–3 carbon atoms, lower alkoxy, aryl, araloxyl, hydroxyl, carboxyl carboxyalkoxy, araloxycarbonylalkosyl, aralkyl, aralkoxycarbonyl,, nitro and lower acylamino, two adjacent mixtures of those mean a substituted halogen, a halo, a nitro and a lower acylaminogroup, two adjacent mixtures of those mean a substituted halogen, a halo, a nitro and a lower acylamino, two adjacent mixtures of the mean are substituted halogen, oannelirovanny residue; X is a sulfur atom or an imino group substituted by a residue of lower alkyl, arshta, and acyl; And the residue of glycocol, alanine, valine, leucine, glycerol, isoleucine, phenylalanine, tyrosine or 0-acetylthirazine, the residue of alanyl-alanine or osso residue of alanyl-alanyl-alanine; This is linked to the nitrogen atom of the amino acids of the A groups of the C — Cg-alkoxycarbonyl, C, —C — alkylsulfonyl 96 4 groups of formyl, acetyl or diphenylamino carbonyl of the O —CO — CH —CH —COOH group, or —C — O — C N / gO-SNZ
    公开号:SU1184444A3
    申请号:SU792853140
    申请日:1979-12-19
    公开日:1985-10-07
    发明作者:Бергер Дитер；Браун Франц；Гютляйн Вегнер；Кур Манфред；Вернер ВОЛЬФГАНГ
    申请人:Берингер Маннхайм Гмбх (Фирма)；
    IPC主号:
    专利说明:

    -C-D
    the remainder
    "..about
    C - g
    upa -S-, -C-0 -C-S-.
    -C-, -S- or -C CH-C-;
    D is a valence trait or C, -Cj-alkylene;
    E is phenyl, 1-naphtsh1, 2-naphthyl, 2-furyl, 8-quinolyl, 2-thienyl, 3-pyridyl, cyclohexyl or H-piperidyl;
    , R, Rj and Rg may be the same or different and denote hydrogen, straight or branched C2-alkyl, C (-C3-alkoxy, nitro, halogen, di-C, -C3-alkylamino, acetylamino, hydroxyl, benzyloxy, HYDROXY-C-Szalkoxy-C | -Cz-alkoxy, methoxy-C-C-alkoxy-C (-Cz-alkoxy, hydroxy-C, -C J-.alkksh.
    halogen-C-C3-alkyl, cyano ,. carboxyl, C, -Cj-alkoxycarbonyl, benzyloxycarbonyl, carbamoyl, dis-C, -C, -alkylcarbamoyl, carboxyl-C, -C-alkyl, carboxyl-C, -C-alkoxy, carboxyl-C-C-alkylamino or phenyl ,
    The following ratio of component.%:
    0.2-9.0
    Chromogen Buffer Solution Else
    2. A diagnostic agent according to claim 1, characterized in that it further comprises a chemical in the amount of 8.9-28.4 wt.%
    3. A diagnostic agent according to Claim 1, characterized in that it further comprises an oxidizing agent in an amount of 1.9-9.0 wt.%.
    4. The diagnostic agent according to claim 1, characterized in that it further comprises a herbal supplement in an amount of 78.5 May. %
    one
    This invention relates to clinical biochemistry, namely to a diagnostic tool for determining leukocytes in the urine.
    The purpose of the invention is to increase the specificity of an agent for determining leukocytes in the urine.
    P r and m er. The filtered paper is sequentially sewn with the following solutions and then dried at 60 ° C.
    Solution 1.
    Disodium tetraborate decahydrate, g1.91
    Distilled water, ml 30 The solution is adjusted to pH 8.0 with 0.1 and hydrochloric acid and the total volume is adjusted to 100.0 ml with distilled water. Solution 2.
    (Benzyloxycarbonyl) -b-alanyloxy-indole, mg 33.8
    Acetone, ml To. General; volume of 100.0.
    A colorless test paper is obtained which, when immersed in urine-containing urine, depending on the concentration of leukocytes, stains in color from light turquoise to blue. You can detect 5,000 leukocytes in 1 μl of urine in 2 minutes; 1000 white blood cells in 1 μl of urine for 6 minutes; 500 leukocytes in 1 μl of urine per 10 minutes; 200 white blood cells in 1 µl of urine in 15 min.
    The sensitivity of the test is 200 leukocytes. Per 1 μl. Evaluation can also be performed using remission photometry at 620 nm.
    Test paper with similar properties (sensitivity 200-2000 leukocytes per 1 μl) is obtained if, instead of (benzyloxycarbonyl 1) -b-alanyloxy-indole, the following substrates are used, it is also possible to give a color from light blue to blue paper and when immersed, urine containing leukocytes: (benzsuxycarbonyl) -l-al nyloxy-4-methyl-indole; (benzyloxycarbonyl) -l-al-nyloxy-5-methyl-indole; (benzyloxycarbonyl) -L-al-nyloxy-6-methyl-indole; (benzyloxycarbonyl) -l-al-nyloxy-7-methyl-indole; 3-tN- (benzyloxycarbonyl) -L-allyloxy-4,7-dimethyl-indole; (benzyloxycarbonyl) -L-al-nyloxy-4-chloro-indole; (benzyloxycarbonyl) -L-al-nyloxy-5-bromo-indole; (Benzyloxycarbonyl) -l-al-nyloxy-6-chloro-indole. Coloring from colorless to purple: “(toluene-4-sulfonyl) -b-alanyloxy-4-chloro-5-bromo-indole; (toluene-4-sulfonyl) -b-alanyloxy | -4, 5,7-trichloro-indole; (toluene-4-sulfonyl) -, - alanyloxy-4-chloro-5-bromo-7-me indole; (tolu-4-sulfonyl) -l-alanyloxy-5-hydroxyindole; (benzyloxycarbonyl) -l-allyloxy-5-methoxy-indole; (toluene-4-sulfonyl) -L-alanyloxy 3-5-benzyloxy-indole; (toluene-4-sulfonyl) -L-alanyloxy1-4 carboxyindole; - city (toluene-4-sulfonyl) -L-alanyloxy-4-benzyl-carbox indole; (toluene-4-sulfonyl) -L-alanyloxy-5-carboxy-methoxyj-indole; 3-H- (toluene-4-sulfonyl) -b-alanypoxy-3-benzyloxycarbonyl-meth: 5th and VD ° - (toluene-4-sulfonyl) -L-alanyloxyT-b-nitro-indole; (toluene-4-sulfonyl) -L-alanyloxy-6-acetylamino-indole (toluene-4-sulfonyl) -L-alanyloxy 3-benzene (§ / -indole, Coloring from colorless to friable: 1-methyl-3-LN- (Benzyloxycarbo-L-alanyloxy-1-indole; 1-benzyl-3-N- (tolu-4-ssub NIL) -L-alanyloxy-indole; 1-phenyl-3-N- (toluene-4-sulphyl) -L -alanyloxy-indol. 44 Coloring from colorless to blue-green: 1-acetyl-Z-N- (, toluene-4-sulfonyl) -b-alanyloxy1-indole. Coloring from colorless to red: 1-benzoyl-3-H - (toluene-4-sulfonyl) -b-alanyloxy | -indole. Coloring from colorless to violet: (benzyloxycarbonyl a) -glycyloxyP-indole; (toluene-4-sulfonyl) -glycyloxy-indole; 3- (N- (toluene-2-sulfonyl) -b-alanyloxy-indole:, (toluene-3-sulfonyl) -L-alanyloxy - indole; (toluene-4-sulfonyl) -b-alanyloxy-indole; 3-N- (toluene-4-sulfonyl) -0-alanyloxyT-indole; 3-K- (benzyloxycarbonl) -0, L-alanyloxy-indole; (Benzyloxycarbonyl) -L-valyloxy-indole; (toluene-4-sulfonyl) -L-valyloxy-indole; (benzyloxycarbonyl) -L-leucyloxy-indole; (benzyloxycarbonyl) -L-isolecyloxy-indole; 3- N- (benzyloxycarbonyl) -L-phenylalanyloxy3-indole; (toluene-4-sulfonyl) -L-fesh lalanyloxy-indole; . (acetyl-L-tyrosyloxide) -indole; 3- (N-benzoyl-L-tyrosyloxide) -indole; (benzyloxycarbonyl) -L-tyrosyloxy-indole; (toluene-4-sulfonyl) -L-tyrosyloxy-indole; (toluene-4-sulfonyl) -0-acetyl-L-tyrosyl-indole; (benz shoxicarbonium) -L-alanyl-L-alaniloxio-alder; (toluene-4-sulfonyl) -D-alanyl-L-alaminoxy-indol; (benzyloxycarbonyl) -L-alanyl-L-alanyl-alanyl-L-oxy-indole; 3-s-Stoluuol-4-sulfonyl) -p-alanch I-D-alanyl-L-alenchl-oxy -indole; 3- (N-formyl-L-alaminoxy) -indole; 3- (N-acetyl-L-alaminoxy) -indole; 3- (N-cycenyl-L-alaminoxy) -indole; 3- (N-benzoyl-B, L-alanyloxy) -indole;
    3- (L-phthaloyl-b-alanyloxy) -indole (ethoxycarbonyl) -b-alanyloxy-indole;
    3-Gk- (tert-butyloxycarbonyl) -L1-alanyloxyJ-indole;
    (3, 6-dioxane-n-heptyloxycarbonyl) -b-alanyloxy-indole;
    (cyclohexyloxycarbonyl) -b-alanyloxy-indole;
    3- N- (phenyloxycarbonyl) -L-alanyoxy-indole;
    (4-methyl-benzyloxycarbonyl) -L-alenyloxy-J-indole;
    3-fN- (4-methoxy-benzyloxycarbo NRL J-L-alanyloxy j-indole;
    3-Gy- (4-nitro-benzyloxycarboL .-.
    NIL) -L-ala-nioxy-J-indole;
    3-TN-FN- (piperidine) -oxycarbonyl-l-alanyloxy-indole;
    3-Hfuryl- (2) -methoxycarbonyl-1-alanyloxy-indole;
    Z-Gy-Gtienil- (2) -methoxycarbonyl-gananyloxy-INDol;
    Z-Gy- (benzylthiocarbonyl) -b-alanyloxy-indole;
    3-Hm- (methanesulfonyl) -b-alanyloxy1-indole;
    3-y- (benzylsulfonyl) -j-alanshloxn-indole;
    3-to- (benzenesulfonyl) -b-alanyl-T-indole;
    (4-bromo-benzolylfcnshI) -L-linearoksy-indol;
    (4-nitro-benzenesulfonyl) -L-alanyloxy-indole;
     . J
    (4-dimethylamino-benzenesulfonyl) -b-alanyloxy-indole;
    (4-acetylamino-benzenesulfonyl) -b-alanyloxy} -indole;
    3-N- (4-n-butyl-benzenesulfonyl-b-alanyloxy-indole;
    (4-tert-butyl-benzenesulfonyl) -b-alanyloxy-indole;
    (4-n-octyl-benzenesulfonyl) -b-alanyloxy-indole;
    (4-hydroxy-benzene sulfonyl) -b-alanyloxy-indole;
    (4-methoxy-benzenesulfonyl) -b-alanyloxy-indole;
    (4-benzyloxy-benzenesulfonyl) -b-alanyloxyJ-indole;
    3-Gy- 4- (2 -oxy-ethoxy) -benzenesulfonyl-L-alanyloxy (-indole;
    3-Hn-4 - (3 -oxy-5-hydroxy-n-pentyloxy) -benzenesulfonyl-L-alanyloxy J-indole;
     4 - (3, d-ixi-n-reptschioxy) -benzenesulfonyl-1-b-alanyloxyT-indole,
    H-W-4 - (2 -oxy-ethyl) -benzenesulfonyl-L-alanyloxyJ-indole;
     - (2 - | 4-nitro-benzyl-T-ethyl) -benzolylphosphine-L-alanyloxy J -indol;
     -ft- (2-chloro-ethyl) -benzenesulfonyl -b-alanyloxy-indole;
    (4-adhethyl-benzenesulfonyl) -L-alanyloxy-indole;
    (4-cyano-benzenesulfonyl) -b-alanyloxy-indole;
    (4-carboxy-benzenesulfonium-L-alanyloxy-indole;
    (4-methoxycarbonyl-benzenesulfonyl) -b-alansh10xy1-indole;
    (4-benzyloxycarbonyl-benzenesulfonyl) -b-alanyl-oxy1-indole;
    (4 -carbamoyl-benzenesulfonyl) -L-alanyloxyJ-indole;
    3- | m- (4 - (dimesh1carbamoyl) -bunzolsulfonishP-L-alanyloxyT -indole;
    (4 -carboxymethyl-benzenesulfonyl) -L-alanyloxyT-indole;
    3- N- (4-carboxymethoxy-benzenesulfonyl) -b-alanyloxy-indole;
    (4-carboxyethylamino-benzenesulfonyl) -b-alanyloxy-indole;
     - (Benztoxycarbonyl-methyl amino) -benzene sulfonyl-L-alanyloxy-indole;
    3- N- (4-fluoro-benzenesulfonyl) -b-alanyloxy-indole;
    (4-fluorosulfonyl-benzenesulfonyl) -L-alanyloxy-indole;
    3-rN- (4 -sulfamoyl-benzenesulL.
    fonil) -L-alaminoxy-J-indole;
    3-M-metsh-1- K- (toluene-4-sulfonyl) -b-alanx 1 Oxy 3 -indole;
    3-N-acetyl-L- (toluene-4-sulfonyl) -L-alanyloxy-indole;
    (2, 4, b-trimethyl-benzenesulfonyl) - -alanyloxy-indole;
    (biphenyl-4-sulfonyl) -b-alanyloxy-indole;
    (naphthalene-2 -sulfonyl) -L-alanyloxy-indole;
    (4-acetylamino-naphthalene-L-sulfonyl) -L-alanyloxy-indole;
    (5-dimethylamino-naphthalene-1 -sulfonyl) -b-alanch1oxy-indole
    (xinolin-8-sulfonyl) -L-alanyloxy-indole;
    (pyridin-3-sulfonyl) -L-alanyloxy-indole; (2-nitro-benzenesulfonyl) -b-alanyloxy indole. Yellow to green staining: with (l -methyl-2-benzoyl-vinyl) -L-apanyloxyZ-indole; . (5, 5-dimethyl-3-oxo-cyclohexen-1 -yl) -alanyloxy-indole; (diphenylcarbamoyl) -l-alanyl-10 oxy 3 -indole; (4-nitrobenzyl) phosphoryl-L-alanyloxy-indole; 3- {N-flH- (4-bromobenzyl) -phosphoryl-L-alanyloxy} -indole; 3-p1- (toluene-4-sulfonyl) -b-alanyloxy-5-benz-1-indole; (toluene-4-sulfonyl) -b-alanyloxy-5-phenyl-indole; (toluene-4-sulfonyl) -b-alanyloxy-5-methoxy-indole.
    权利要求:
    Claims (4)
    [1]
    1. DIAGNOSTIC MEANS FOR DETERMINING LEUKOCYTES IN URINE, containing a chromogen and a buffer solution, characterized in that, in order to increase its specificity, it contains an indoxyl or thionoxyl ether of the general formula where R,, Rj, R 3 , ® · 4 as a chromogen hydrogen, halogen, alkyl with 1-3 carbon atoms, lower alkoxyl, aryl, araloxyl, hydroxyl, carboxyl, carboxyalkoxyl, araloxycarbonylalkosyl, aralkyl, aralkoxycarbonyl, nitro lower acylamino group, two adjacent substituents mean substituted benzene halogen nny residue;
    X is a sulfur atom or an imino group substituted by a residue of lower alkyl, aryl and acyl;
    A is the residue of glycocol, alanine, valine, leucine, glycerol, isolecin, phenylalanine, tyrosine or 0-acetyl tyrazine, the residue of alanyl-alanine or a residue of alanyl-alanyl-alanine;
    B - a group of C 4 -C 6 alkoxycarbonyl, C, —C ^ alkylsulfonyl formyl, acetyl or diphenylaminocarbonyl group bonded to the nitrogen atom of the amino acids specified in A
    ABOUT
    V Ί -so-sn 2 -sn 2 -soon, or —С _ 0 _ С 2 Нц. About SPZ ">
    or balance
    C-d
    0 with w
    -S-, -C-O, -CS-, 'Sn 3 ' o '-S- or —C = CH-C-;
    group h-s-, valency line or
    C ( -C 3 alkylene;
    E is phenyl, 1-naphthyl, 2-naphthyl, 2-furyl, 8-quinolyl, 2-thienyl, 3-pyridyl, cyclohexyl or N-piperidyl;
    R 5 , R & R and Rg may be the same or different and denote hydrogen, straight or branched C 1 -Cg alkyl, C (-Cz ~ alkoxy, nitro, halo'gen, di-C, -Cj-alkylamino, acetylamino, hydroxyl, benzyloxy, hydroxy -Cf-Cj alkoxy — C (—C e -alkoxy, methoxy-C, -C 3 -alkoxy-C ( -C 3 -alk- * -oxy, hydroxy-C, -C -Alkyl, halogen-C ^ -C 3 -alkyl, cyano ,, carboxyl, C G -C 3 ~ alkoxycarbonyl ~ benzyl, benzyloxycarbonyl, carbamoyl, dis-C, -C, -alkylcarbamoyl, carboxyl-C, -C 3 ~ -alkyl, carboxyl-C, -C 3- alkoxy, carboxyl-C, -C 3 -alkylamino or phenyl, in the following ratio, wt.%:
    Chromogen 0.2-9.0
    Buffer solution Else
    [2]
    2. The diagnostic tool according to π.1, characterized in that it additionally contains a wetting agent in the amount of 8.9-28.4 wt.%.
    [3]
    3. The diagnostic tool according to claim 1, characterized in that it further comprises an oxidizing agent in an amount of 1.9-9.0 wt.%.
    [4]
    '4. The diagnostic tool according to claim 1, characterized in that it further comprises a galenic additive in an amount of 78.5 may. Z.
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    同族专利:
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    DE2960857D1|1981-12-03|
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    PL127198B1|1983-10-31|
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    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    DE2854987A|DE2854987A1|1978-12-20|1978-12-20|DIAGNOSTIC AGENTS FOR DETECTING PROTEOLYTIC ENZYMS AND CHROMOGENS SUITABLE FOR THIS|
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