Diaminothiazoles

专利摘要:
Novel diaminothiazoles are selective inhibitors of Cdk4. The compounds, and pharmaceutically acceptable salts and esters thereof, are antiproliferative agents useful for the treatment or control of solid tumors, particularly breast, colon, lung and prostate tumors. Also disclosed are pharmaceutical compositions containing these compounds and intermediates useful in the preparation of these compounds.
公开号:KR20030069222A
申请号:KR10-2003-7009679
申请日:2002-01-18
公开日:2003-08-25
发明作者:천리；딩칭제；길즈파이폴；김경진；러비앨런존；매코마스와런윌리엄；멀린존길포일주니어；페로타애고스티노
申请人:에프. 호프만-라 로슈 아게；
IPC主号:

专利说明:

Diaminothiazole {DIAMINOTHIAZOLES}
[1] The present invention relates to novel diaminothiazoles of the formula:
[2]
[3] These compounds inhibit cyclin dependent kinase 4 (Cdk4) and are selective for Cdk2 and Cdk1. These compounds, and their pharmaceutically acceptable salts and esters, have antiproliferative activity and are useful for the treatment or control of cancer, especially solid tumors. The present invention also relates to pharmaceutical compositions containing such compounds, and methods of treating or controlling cancer, most particularly the treatment or control of breast, lung, colon and prostate tumors. Finally, the present invention also relates to novel intermediate compounds useful for the preparation of compounds of formula (I).
[4] Uncontrolled cell proliferation is a hallmark of cancer. Cancerous tumor cells usually inflict some form of damage to genes that directly or indirectly regulate the cell division cycle.
[5] Cell progression through various aspects of the cell cycle is regulated by a series of multienzyme complexes consisting of regulatory proteins, cyclins, and kinases. Such kinases are called cyclin dependent kinases (Cdks). Cdk is expressed throughout the cell cycle, but the level of cyclin varies depending on the stage in the cell cycle.
[6] The transition from the G ₁ aspect to the S aspect is regulated by the complex of Cdk4 and cyclin D. The complex phosphorylates tumor suppressor protein retinoblastoma (pRb), releases transcription factor E2F, and allows the expression of the genes required in the S aspect (Nevins, JR Science 1992, 258, 424-429). Activity blocking of the Cdk4 / cyclin D complex stops the cell cycle in the G ₁ aspect. For example, proteins with ^INK4 , including p16 ^INK4a , which block kinase activity of the Cdk4 / cyclin D complex, cause a stop at G ₁ (Sherr, CJ Science 1996, 274, 1672-1677).
[7] Recent experiments show that the complex of Cdk4 and cyclin D3 also plays a role in cell cycle progression through the G ₂ modality. Inhibition of this complex by p16 or with dominant negative Cdk4 results in arrest in the G ₂ modality in cells that do not express pRb (Gabrielli BG et al . , J. Biol. Chem. 1999, 274, 13961-13969).
[8] Numerous defects in the pRb pathway have been shown to be involved in various cancers. For example, overexpression of Cdk4 has been observed for hereditary melanoma (Webster, KR Exp . Opin. Invest. Drugs 1998, 7, 865-887); Cyclin D is overexpressed in many human cancers (Sherr, CJ Science 1996, 274,1672-1677); p16 is mutated or deleted in many tumors (Webster, KR Exp . Opin. Invest. Drugs 1998, 7, 865-887); pRb function is lost through mutation or deletion in many human cancers (Weinberg, RA Cell 1995, 81, 323-330). Defects in this pathway have also been shown to affect prognosis. For example, loss of p16 is correlated with poor prognosis in non-small cell lung carcinoma (NSCLC) and malignant melanoma (Tsihlias, J. et al. Annu. Rev. Med. 1999, 50, 401-423 ).
[9] Factors in the pathway, due to the relationship between the Cdk4 / cyclin D / pRb pathway and human cancer through its role in regulating cell cycle progression from the G ₁ to S modality, and the potential therapeutic benefits resulting from the modulation of the pathway There has been considerable interest in medicaments that inhibit or enhance the activity. For example, overexpression or addition of proteins involved in this pathway, antibodies and antisense oligonucleotides have been used to show effects on cancer cells. See, eg, Lukas, J. et al ., Nature 1995, 79, 573-582; Nevins, JR Science 1992, 258, 424-429; Lim, IK et al., Molecular Carcinogenesis 1998, 23, 25-35; Tam, SW et al. Oncogene 1994, 9, 2663-2674; Drriscoll, B. et al . Am. J. Physiol. 1997, 273 ( Lung Cell. Mol. Physiol. ), L 941 to L949; And Sang, J. et al . , Chin. Sci. Bull. 1999, 44, 541-544. Thus, there is a large amount of literature demonstrating the use of compounds that inhibit targets in the Cdk4 pathway as antiproliferative agents.
[10] Accordingly, it is desirable to identify chemical inhibitors of Cdk4 kinase activity. It is particularly desirable to identify one or more small molecule compounds for tumor treatment that can be readily synthesized and effective in inhibiting Cdk4 or Cdk4 / cycline complexes.
[11] There are several examples of small molecule inhibitors of cyclin dependent kinases including Cdk4 (Rosania, GR et al . Exp. Opin. Ther. Patents 2000, 10, 215-230). Some of these compounds inhibit multiple targets.
[12] For example, Flavoridol (Aventis) is in Phase II clinical trials for lymphoma and multiple myeloma and for the treatment of solid tumors:
[13]
[14] It inhibits Cdk1, Cdk2 and Cdk4 and blocks cells in both G1 and G2 aspects. It is also a weak inhibitor of PKC and EGFR (Senderowicz, AM et al . , J. Natl. Cancer Inst. 2000, 92, 376-387).
[15] WO 9716447 (Mitotix) discloses the following compounds related to flavopyridols:
[16]
[17] Some of these compounds are mentioned to inhibit Cdk4.
[18] WO 9943675 and WO 9943676 (Hoechst) disclose the following purine derivatives which are mentioned to inhibit Cdk2 and Cdk4:
[19]
[20] WO 9833798 (Warner-Lambert) discloses the following pyridopyrimidines:
[21]
[22] These compounds are said to inhibit cyclin dependent kinases Cdk1, Cdk2 and Cdk4. Some of these compounds also inhibit receptor tyrosine kinases PDGFR and EGFR and c-Src, a Src protein kinase in cells.
[23] WO 9909030 (Warner-Lambert) discloses naphthyridinone which inhibits PDGFR, FGFR, c-Src, and cyclin dependent kinases Cdk1, Cdk2, and Cdk4:
[24]
[25] WO 0039101 (AstraZeneca) discloses diaminopyrimidines that inhibit Cdk4 and FAK3:
[26]
[27] WO 0012485 (Zeneca) discloses diaminopyrimidines that inhibit Cdk4 and FAK3:
[28]
[29] WO 9924416 (Bristol-Myers Squibb) discloses aminothiazole inhibitors of the formula:
[30]
[31] These compounds inhibit Cdk1, Cdk2, and Cdk4.
[32] WO 9921845 (Agouron) discloses diaminothiazole inhibitors of Cdk1, Cdk2 and Cdk4 having the structure:
[33]
[34] (Wherein R1 and R2 are ring systems). The patent application points out that when the R2 ring system has no ortho substituents, the compounds lack potency and selectivity as inhibitors of Cdk4.
[35] Finally, WO 0075120 (Agouron) discloses diaminothiazole inhibitors of protein kinases including VEGF-R, FGF-R, CDK complex, TEK, CHK1, LCK, and FAK, having the following structure:
[36]
[37] It is desirable to provide small molecule inhibitors of Cdk4 that are selective for other Cdks. In other words, the small molecule significantly inhibits Cdk4 more than Cdk1 and Cdk2 (at least 10 times, preferably 100 times). The above parameters are preferred because of the potential accompanying toxicity and other undesirable complications that may follow due to the inhibition of multiple targets. Thus, for the purposes of the present invention, inhibition of Cdk2 and Cdk1 is monitored to determine the inhibition selectivity of Cdk4. Compounds exhibiting selectivity for Cdk2 and Cdk1 are expected to have a better safety profile than compounds that are not selective between Cdk4, Cdk2 and Cdk1.
[38] There is a continuing need for readily synthesized small molecule compounds that are specific inhibitors of Cdk4 for the treatment or control of one or more solid tumors. It is an object of the present invention to provide such compounds, compositions containing such compounds, and methods of using said compounds in the treatment or control of breast, colon, lung and prostate tumors.
[39] The present invention relates to novel diaminothiazoles capable of selectively inhibiting the activity of Cdk4. The compounds are useful for the treatment or control of cancer, in particular for the treatment or control of solid tumors. In particular, the present invention relates to compounds of formula (I), or pharmaceutically acceptable salts or esters thereof:
[40]
[41] Wherein R ¹ is selected from the group consisting of lower alkyl, H, cycloalkyl, COR ⁹ , and SO ₂ R ¹⁰ which may be optionally substituted with a group selected from OR ⁶ , cycloalkyl, and NR ⁷ R ⁸ ;
[42] R ² is selected from the group consisting of H, F, Cl, and CH ₃ ;
[43] R ³ , R ⁴ and R ⁵ are each independently lower alkyl, H, OR ¹¹ , NR ¹² R ¹³ , halogen, NO ₂ , CONR ⁶ R which may be optionally substituted with a group selected from OR ⁶ and NR ⁷ R ^{8 9} , NHSO ₂ R ¹⁴ , CN, S-lower alkyl, OCF ₃ , and OCHF ₂ ,
[44] Or alternatively, R ³ and R ⁴ taken together with a bond and two carbons between them from the benzene ring (D) to which R ³ and R ⁴ are attached are a ring system having up to two additional rings It can form, and wherein said rings each having a 5 to 7 ring atoms, and the ring attached to the benzene ring (D) includes any one or more heteroatoms, and optionally substituted by lower alkyl, with the proviso that, R ³ and R ⁴ are not simultaneously -OCH _3, also R ³ is -NO ₂ days when R ⁴ is not a -Cl;
[45] R ⁶ and R ⁹ are independently selected from the group consisting of lower alkyl, optionally substituted with OH and halogen, and H;
[46] R ⁷ and R ⁸ are independently selected from the group consisting of lower alkyl, optionally substituted with OR ⁶ , and H,
[47] Alternatively, R ⁷ is H and R ⁸ is OH, or alternatively, NR ⁷ R ⁸ may optionally form a ring having 5 to 6 atoms, wherein the ring is one or more additional hetero Optionally substituted by a group consisting of lower alkyl optionally containing one or more OR ⁶ and optionally substituted with OH by itself;
[48] R ¹⁰ is selected from the group consisting of lower alkyl, which may be optionally substituted by one or more chlorine or fluorine, and NH ₂ ;
[49] R ¹¹ is selected from the group consisting of OR ⁶ , COOH, halogen and lower alkyl optionally substituted with a group selected from NR ¹⁵ R ¹⁶ , and H;
[50] R ¹² and R ¹³ are independently selected from the group consisting of lower alkyl, H, COR ¹⁷ , and SO ₂ R ¹⁸ which may be optionally substituted with a group selected from OR ⁶ , COOH and NR ¹⁵ R ¹⁶ , provided that R is Only one of ¹² and R ¹³ is COR ¹⁷ or SO ₂ R ¹⁸ , or alternatively NR ¹² R ¹³ may optionally form a ring having 5 to 6 atoms, the ring having one or more additional heteroatoms Optionally substituted by a group consisting of one or more OR ⁶ and lower alkyl, which may itself be optionally substituted with OH;
[51] R ¹⁴ is lower alkyl;
[52] R ¹⁵ and R ¹⁶ are independently selected from the group consisting of lower alkyl, optionally substituted with OH, and H,
[53] Or alternatively NR ¹⁵ R ¹⁶ may optionally form a ring having 5 to 6 atoms, the ring optionally comprising one or more additional heteroatoms and one or more OR ⁶ and itself to be optionally substituted with OH Optionally substituted by a group consisting of lower alkyl;
[54] R ¹⁷ is selected from the group consisting of lower alkyl, which may be optionally substituted with groups selected from OH, COOH, and NR ¹⁵ R ¹⁶ , and H;
[55] R ¹⁸ is lower alkyl).
[56] The present invention also relates to pharmaceutical compositions containing a therapeutically effective amount of one or more compounds of formula (I) and a pharmaceutically acceptable carrier or excipient.
[57] The present invention also relates to a method of treating solid tumors, in particular breast or colon tumors, by administering to a human patient in need thereof an effective amount of a compound of formula (I), a salt and / or an ester thereof.
[58] The present invention also relates to novel intermediate compounds useful for the preparation of compounds of formula (I).
[59] As used herein, the following terms will have the following definitions.
[60] "Cycloalkyl" means a non-aromatic, partially or fully saturated cyclic aliphatic hydrocarbon group containing 3 to 8 atoms. Examples of cycloalkyl groups include cyclopropyl, cyclopentyl and cyclohexyl.
[61] An “effective amount” means an amount effective to prevent, alleviate or ameliorate the symptoms of a disease or to prolong the survival of a treated subject.
[62] "Halogen" means fluorine, chlorine, bromine or iodine.
[63] "Hetero atom" means an atom selected from N, O and S.
[64] "IC ₅₀ " refers to the concentration of a particular compound according to the invention required to inhibit 50% of a particular measurement activity. IC ₅₀ may in particular be measured as described in Example 125 and below.
[65] "Lower alkyl" refers to a straight or branched saturated aliphatic hydrocarbon having 1 to 6 carbon atoms, preferably 1 to 4 carbon atoms. Representative lower alkyl groups include methyl, ethyl, propyl, isopropyl, butyl, t-butyl, 2-butyl, pentyl, hexyl and the like.
[66] "Pharmaceutically acceptable esters" are commonly esterified Formula I having carboxyl groups that retain biological effectiveness and properties of the compounds of Formula I and degrade in vivo (in organic) to the corresponding active carboxylic acid. The compound of. Examples of ester groups that decompose (in this case, hydrolyze) with the corresponding carboxylic acid (R ²⁴ C (═O) OH) in vivo include lower alkyl esters, wherein R can be substituted with NR ²⁵ R ^{26 25} and R ²⁶ are lower alkyl, or NR ²⁵ R ²⁶ taken together forms a monocyclic aliphatic heterocycle such as pyrrolidine, piperidine, morpholine, N-methylpiperazine, etc.); Acyloxyalkyl esters of the formula R ²⁴ C (= 0) OCHR ²⁷ OC (= 0) R ²⁸ , wherein R ²⁷ is hydrogen or methyl and R ²⁸ is lower alkyl or cycloalkyl; Carbonate esters of the formula R ²⁴ C (= 0) OCHR ²⁷ OC (= 0) OR ²⁹ , wherein R ²⁷ is hydrogen or methyl and R ²⁹ is lower alkyl or cycloalkyl; Or an aminocarbonylmethyl ester of the formula R ²⁴ C (= 0) OCH ₂ C (= 0) NR ²⁵ R ²⁶ , wherein R ²⁵ and R ²⁶ are hydrogen or lower alkyl or taken together with NR ²⁵ R ²⁶ is monocyclic Aliphatic heterocycles such as pyrrolidine, piperidine, morpholine, N-methylpiperazine and the like).
[67] Examples of lower alkyl esters are methyl, ethyl, and n-propyl esters and the like. Examples of lower alkyl esters substituted with NR ¹⁹ R ²⁰ are diethylaminoethyl, 2- (4-morpholinyl) ethyl, 2- (4-methylpiperazin-1-yl) ethyl ester and the like. Examples of acyloxyalkyl esters are pivaloxymethyl, 1-acetoxyethyl, and acetoxymethyl esters. Examples of carbonate esters are 1- (ethoxycarbonyloxy) ethyl and 1- (cyclohexyloxycarbonyloxy) ethyl ester. Examples of aminocarbonylmethyl esters are N, N-dimethylcarbamoylmethyl and carbamoylmethyl esters.
[68] Further information regarding the use and illustration of esters for the delivery of pharmaceutical compounds is given in Design of Prodrugs. Bundgaard H ed. (Elsevier, 1985). See also: [H. Ansel et al., Pharmaceutical Dosage Forms and Drug Delivery Systems (6th Ed. 1995), pp. 108-109; Krogsgaard-Larsen et al., Textbook of Drug Design and Development (2d Ed. 1996), pp. 152-191].
[69] "Pharmaceutically acceptable salts" refer to conventional acid addition salts or base addition salts which retain the biological effectiveness and properties of the compounds of formula (I) and are formed from suitable non-toxic organic or inorganic acids or organic or inorganic bases. Examples of acid addition salts include those derived from inorganic acids such as hydrochloric acid, hydrobromic acid, hydroiodic acid, sulfuric acid, sulfamic acid, phosphoric acid and nitric acid, and organic acids such as p-toluenesulfonic acid, salicylic acid, methanesulfonic acid, oxalic acid, succinic acid, citric acid, And those derived from malic acid, lactic acid, fumaric acid and the like. Examples of base addition salts include those derived from ammonium, potassium, sodium, and quaternary ammonium hydroxides such as tetramethylammonium hydroxide. Chemical modification of pharmaceutical compounds (ie, drugs) to salts is a technique well known to pharmaceutical chemists to obtain improved physical and chemical stability, hygroscopicity, flowability and solubility of the compounds. See, eg, H. Ansel et al., Pharmaceutical Dosage Forms and Drug Delivery Systems (6th Ed. 1995), pp. 196 and 1456-1457.
[70] “Pharmaceutically acceptable”, such as in pharmaceutically acceptable carriers, excipients, etc., means pharmacologically acceptable and substantially nontoxic to the subject to which the particular compound is administered.
[71] "Substituted" as in substituted alkyl, means that the substitution may occur at one or more positions, and unless stated otherwise, the substituents at each substitution site are independently selected from the specified selection.
[72] A "therapeutically effective amount" means the amount of one or more compounds of Formula (I), or pharmaceutically acceptable salts or esters thereof, that significantly inhibit and / or prevent differentiation of human tumor cells, including human tumor cell lines. do.
[73] In one embodiment, the invention relates to a compound of formula (I), or a pharmaceutically acceptable salt or ester thereof:
[74] [Formula I]
[75]
[76] Wherein R ¹ is selected from the group consisting of lower alkyl, H, cycloalkyl, COR ⁹ , and SO ₂ R ¹⁰ which may be optionally substituted with a group selected from OR ⁶ , cycloalkyl, and NR ⁷ R ⁸ ;
[77] R ² is selected from the group consisting of H, F, Cl, and CH ₃ ;
[78] R ³ , R ⁴ and R ⁵ are each independently lower alkyl, H, OR ¹¹ , NR ¹² R ¹³ , halogen, NO ₂ , CONR ⁶ R which may be optionally substituted with a group selected from OR ⁶ and NR ⁷ R ^{8 9} , NHSO ₂ R ¹⁴ , CN, S-lower alkyl, OCF ₃ , and OCHF ₂ ,
[79] Or alternatively, R ³ and R ⁴ taken together with a bond and two carbons between them from the benzene ring (D) to which R ³ and R ⁴ are attached are a ring system having up to two additional rings Wherein each of the rings has 5 to 7 atoms, and the ring attached to the benzene ring (D) optionally contains one or more hetero atoms, optionally substituted by lower alkyl,
[80] However, R ³ and R ⁴ are not simultaneously -OCH _3, also R ³ is -NO ₂ days when R ⁴ is not a -Cl;
[81] R ⁶ and R ⁹ are independently selected from the group consisting of lower alkyl, optionally substituted with OH and halogen, and H;
[82] R ⁷ and R ⁸ are independently selected from the group consisting of lower alkyl, which may be optionally substituted with OR ⁶ , and, alternatively, R ⁷ is H and R ⁸ is OH,
[83] Or alternatively, NR ⁷ R ⁸ may optionally form a ring having 5 to 6 atoms, said ring optionally comprising one or more additional heteroatoms and optionally substituted with one or more OR ⁶ and itself with OH Optionally substituted by a group consisting of lower alkyl;
[84] R ¹⁰ is selected from the group consisting of lower alkyl, which may be optionally substituted by one or more chlorine or fluorine, and NH ₂ ;
[85] R ¹¹ is selected from the group consisting of OR ⁶ , COOH, halogen and lower alkyl optionally substituted with a group selected from NR ¹⁵ R ¹⁶ , and H;
[86] R ¹² and R ¹³ are independently selected from the group consisting of lower alkyl, H, COR ¹⁷ , and SO ₂ R ¹⁸ which may be optionally substituted with a group selected from OR ⁶ , COOH and NR ¹⁵ R ¹⁶ , provided that R is Only one of ¹² and R ¹³ is COR ¹⁷ or SO ₂ R ¹⁸ , or alternatively NR ¹² R ¹³ may optionally form a ring having 5 to 6 atoms, the ring having one or more additional heteroatoms Optionally substituted by a group consisting of one or more OR ⁶ and lower alkyl, which may itself be optionally substituted with OH;
[87] R ¹⁴ is lower alkyl;
[88] R ¹⁵ and R ¹⁶ are independently selected from the group consisting of lower alkyl which may be optionally substituted with OH, and H, or alternatively NR ¹⁵ R ¹⁶ may optionally form a ring having 5 to 6 atoms Wherein the ring is optionally substituted by a group consisting of one or more additional hetero atoms and optionally one or more OR ⁶ and lower alkyl, which may itself be optionally substituted with OH;
[89] R ¹⁷ is selected from the group consisting of lower alkyl, which may be optionally substituted with groups selected from OH, COOH, and NR ¹⁵ R ¹⁶ , and H;
[90] R ¹⁸ is lower alkyl).
[91] In a preferred embodiment of the compound of formula I, R ¹ is H, CH ₂ CH ₂ OH, CH ₂ CH ₂ CH ₂ OH, CH ₃ CO-, CH (CH ₃ ) ₂ , CH ₂ CH (CH ₃ ) ₂ , Cyclopropylmethyl and CH ₃ . More preferably, R ¹ is H, methyl, CH ₂ CH ₂ CH ₂ OH, or CH (CH ₃ ) ₂ .
[92] In another preferred embodiment of the compounds of formula (I), R ² is selected from the group consisting of H and fluorine, most preferably H.
[93] In another preferred embodiment of the compounds of formula (I), R ³ is selected from the group consisting of OR ¹¹ , lower alkyl, NH ₂ , Cl, F, H, OCHF ₂ , and NO ₂ . More preferably R ³ is OCH ₃ , F, lower alkyl or OCHF ₂ . Most preferably R ³ is F, OCH ₃ or CH ₂ CH ₃ .
[94] In another preferred embodiment of the compound of formula (I), R ⁴ is acetamido, chloro, diethylamino, hydrogen, hydroxy, hydroxyethylamino, [1- (hydroxymethyl) -3-methylbutyl] amino, 1- (3-hydroxymethyl) piperidinyl, 4-hydroxy-1-piperidinyl, methoxy, 2-methoxy-ethylamino, 2-methyl-1-pyrrolidinyl, morpholino, pipepe Selected from the group consisting of lidinyl and pyrrolidinyl. More preferably, R ⁴ is selected from the group consisting of H and CH ₃ O-.
[95] When R ³ and R ⁴ taken together with the benzene ring to which they are attached form a ring system, preferred ring systems are 2-dibenzofuranyl, 1,3-benzodioxol-5-yl, 2,3- Dihydro-1,4-benzodioxin-6-yl, or 3,4-dihydro-2H-1,5-benzodioxepin-7-yl, more preferably 2,3-dihydro-1, 4-benzodioxin-6-yl.
[96] In another preferred embodiment of the compounds of formula (I), R ⁵ is selected from the group consisting of H, OR ¹¹ and F, most preferably H.
[97] In another preferred embodiment of the compound of formula I,
[98] R ¹ is selected from the group consisting of lower alkyl, which may be optionally substituted by OR ⁶ , and H;
[99] R ² is selected from the group consisting of H and F;
[100] R ³ is selected from the group consisting of H, lower alkyl, halogen, NR ¹² R ¹³ , NO ₂ , OCHF ₂ and OR ¹¹ ;
[101] R ⁴ is selected from the group consisting of lower alkyl, H, halogen, and NR ¹² R ¹³ , which may be optionally substituted with OR ⁶ ,
[102] Or alternatively, R ³ and R ⁴ taken together with a bond and two carbons between them from the benzene ring (D) to which R ³ and R ⁴ are attached are a ring system having up to two additional rings It can form, and wherein each said ring has a 5 to 7 atoms, the ring attached to the benzene ring (D) comprises one or more heteroatoms and optionally is optionally substituted by lower alkyl, with the proviso that, R ³ is When -NO ₂ , R ⁴ is not -Cl;
[103] R ⁶ is selected from the group consisting of H, and methyl;
[104] R ¹¹ is selected from the group consisting of OR ⁶ , COOH, halogen and lower alkyl optionally substituted with a group selected from NR ¹⁵ R ¹⁶ , and H;
[105] R ¹² and R ¹³ are independently selected from the group consisting of lower alkyl, H, which may optionally be substituted with a group selected from OR ⁶ , COOH and NR ¹⁵ R ¹⁶ , or alternatively NR ¹² R ¹³ is 5-6 May optionally form a ring having 2 atoms, said ring optionally substituted by a group consisting of one or more additional heteroatoms and optionally consisting of one or more OR ⁶ and lower alkyl optionally optionally substituted with OH ;
[106] R ¹⁵ and R ¹⁶ are independently selected from the group consisting of lower alkyl which may be optionally substituted with OH, and H, or alternatively NR ¹⁵ R ¹⁶ may optionally form a ring having 5 to 6 atoms Wherein the ring optionally comprises one or more additional heteroatoms, optionally substituted by one or more OR ⁶ and lower alkyl, which may itself be optionally substituted with OH.
[107] In the most preferred embodiment of the compound of formula (I),
[108] R ¹ is selected from the group consisting of H, CH ₂ CH ₂ OH, CH ₂ CH ₂ CH ₂ OH, CH ₃ CO-, CH (CH ₃ ) ₂ , CH ₂ CH (CH ₃ ) ₂ , cyclopropylmethyl and CH ₃ Selected;
[109] R ² is selected from the group consisting of H and F;
[110] R ³ is selected from the group consisting of OR ¹¹ , lower alkyl, NH ₂ , Cl, F, H, OCHF ₂ , and NO ₂ ;
[111] R ⁴ is selected from the group consisting of H, and diethylamino;
[112] R ⁵ is H;
[113] R ¹¹ is unsubstituted lower alkyl.
[114] In another most preferred embodiment of the compound of formula I,
[115] R ¹ is selected from the group consisting of H, CH ₂ CH ₂ CH ₂ OH, CH (CH ₃ ) ₂ , CH ₃ , and cyclopropylmethyl;
[116] R ² , R ⁴ and R ⁵ are H;
[117] R ³ is selected from the group consisting of OCH ₃ , F, and CH ₂ CH ₃ .
[118] The following are examples of preferred compounds of formula (I):
[119] a) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (2,3-dihydro-1,4-benzodioxin- 6-day) methanone (Example 24),
[120] b) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (1,3-benzodioxol-5-yl) methanone ( Example 25),
[121] c) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [4- (1-pyrrolidinyl) phenyl] methanone (implemented Example 26),
[122] d) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [4- (1-piperidinyl) phenyl] methanone (implemented Example 27),
[123] e) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [4- (4-morpholinyl) phenyl] methanone, acetate Salt (Example 28),
[124] f) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (3,4-dihydro-2H-1,5-benzodiox Sepin-7-yl) methanone (Example 29),
[125] g) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl]-(4-hydroxyphenyl) methanone (Example 30),
[126] h) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (3-nitrophenyl) methanone (Example 31),
[127] i) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (4-methoxyphenyl) methanone (Example 32),
[128] j) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [4- (diethylamino) phenyl] methanone (Example 33 ),
[129] k) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (2-dibenzofuranyl) methanone (Example 34),
[130] 1) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (3-fluoro-4-methoxyphenyl) methanone (implemented Example 35),
[131] m) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (3-methoxyphenyl) methanone (Example 36),
[132] n) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (3,4-dichlorophenyl) methanone (Example 37),
[133] o) [4-amino-2-[[3-fluoro-4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (1,3-benzodioxol-5- (1) metanon (Example 38),
[134] p) [4-amino-2-[[3-fluoro-4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (3,5-dimethoxyphenyl) methanone (Example 39),
[135] q) [4-amino-2-[[3-fluoro-4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (3-methoxyphenyl) methanone (implemented 40),
[136] r) [4-amino-2-[[4- [4- (1-methylethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (2,3-dihydro-1,4 -Benzodioxine-6-yl) methanone (Example 41),
[137] s) [4-amino-2-[[4- [4- (1-methylethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (1,3-benzodioxol-5- (L) metanon (Example 42),
[138] t) [4-amino-2-[[4- [4- (1-methylethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (3-methoxyphenyl) methanone (implemented 43),
[139] u) [4-amino-2-[[4- [4- (1-methylethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (3,5-dimethoxyphenyl) methanone (Example 44),
[140] v) N- [4-[[4-amino-2-[[4- [4- (1-methylethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] carbonyl] phenyl] Acetamide (Example 45),
[141] w) [4-amino-2-[[4- [4- (1-methylethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] [4- (diethylamino) phenyl] meta Rice field (Example 46),
[142] x) 1-acetyl-4- [4-[[4-amino-5-[(1,3-benzodioxol-5-yl) carbonyl] -2-thiazolyl] amino] phenyl] piperazine (implemented 47),
[143] y) 1-acetyl-4- [4-[[4-amino-5- [4- (diethylamino) benzoyl] -2-thiazolyl] amino] phenyl] piperazine, trifluoroacetate salt (Example 48),
[144] z) [4-amino-2-[[4- [4- (2-hydroxyethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (2,3-dihydro-1, 4-benzodioxin-6-yl) methanone (Example 49),
[145] aa) [4-amino-2-[[4- [4- (2-hydroxyethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] [4- (1-pyrrolidinyl) Phenyl] methanone (Example 50),
[146] bb) [4-amino-2-[[4- [4- (2-hydroxyethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (3-fluorophenyl) methanone ( Example 51),
[147] cc) [4-amino-2-[[4- [4- (2-hydroxyethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (3,5-difluorophenyl) Metanon (Example 52),
[148] dd) [4-amino-2-[[4- [4- (2-hydroxyethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (3-methoxyphenyl) methanone ( Example 53),
[149] ee) [4-amino-2-[[4- (1-piperazinyl) phenyl] amino] -5-thiazolyl] (3-fluorophenyl) methanone (Example 54),
[150] ff) [4-amino-2-[[4- (1-piperazinyl) phenyl] amino) -5-thiazolyl] [4- (1-pyrrolidinyl) phenyl] methanone (Example 55),
[151] gg) [4-amino-2-[[4- (1-piperazinyl) phenyl] amino) -5-thiazolyl] (3-fluoro-4-methoxyphenyl) methanone (Example 56),
[152] hh) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [4- (2-hydroxyethyl) amino-3-nitrophenyl ] Metanon (Example 57),
[153] ii) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4- (1-pyrrolidinyl) phenyl] Metanon (Example 58),
[154] jj) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4- (4-morpholinyl) phenyl] Metanon (Example 59),
[155] kk) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4-[(2-methoxyethyl) amino ] Phenyl] methanone (Example 60),
[156] ll) racemic [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4- [3- (hydroxymethyl ) -1-piperidinyl] phenyl] methanone (Example 61),
[157] mm) racemic [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4- (2-methyl-1- Pyrrolidinyl) phenyl] methanone (Example 62),
[158] nn) (R)-[4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4-[[1- ( Hydroxymethyl) -3-methylbutyl] amino] phenyl] methanone (Example 63),
[159] oo) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4- (4-hydroxy-1-pipepe Ridinyl) phenyl] methanone (Example 64),
[160] pp) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-amino-4- (4-pyrrolidinyl) phenyl] Metanon (Example 65),
[161] qq) (R)-[3-amino-4-[[1- (hydroxymethyl) -3-methylbutyl] amino] phenyl] [4-amino-2-[[4- (4-methyl-1- Piperazinyl) phenyl] amino] -5-thiazolyl] methanone (Example 66),
[162] rr) [4-amino-2-[[3-fluoro-4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (2,3-dihydro-1,4 -Benzodioxine-5-yl) methanone (Example 67),
[163] ss) [4-amino-2-[[3-fluoro-4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [4- (1-pyrrolidinyl) phenyl ] Metanon (Example 68),
[164] tt) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-methoxy-phenyl) -methanone, Phosphoric acid (Example 69),
[165] uu) {4-amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl} (3-methylsulfanyl-phenyl) -methanone (implemented 70),
[166] vv) (4-amino-2- {4- [4- (3-hydroxy-propyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3-fluoro- Phenyl) -methanone (Example 71),
[167] ww) (4-amino-2- {4- [4- (3-hydroxy-propyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3-methoxy- Phenyl) -methanone (Example 72),
[168] xx) 3- (4-Amino-2- {4- [4- (3-hydroxy-propyl) -piperazin-1-yl] -phenylamino} -thiazole-5-carbonyl) -benzonitrile ( Example 73),
[169] yy) (4-amino-2- {4- [4- (3-hydroxy-propyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3-nitro-phenyl ) -Methanone (Example 74),
[170] zz) (4-Amino-2- {4- [4- (3-hydroxy-propyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl) -m-tolyl-methanone (Example 75),
[171] aaa) (4-amino-2- {4- [4- (3-hydroxy-propyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3-ethyl-phenyl ) -Methanone (Example 76),
[172] bbb) {4-amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-ethyl-phenyl) -methanone (Example 77),
[173] ccc) {4-Amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl} -m-tolyl-methanone (Example 78),
[174] ddd) 3- {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazole-5-carbonyl} -benzonitrile (Example 79),
[175] eee) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-fluoro- with hydrogen bromide Phenyl) -methanone (Example 80),
[176] fff) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3,4,5) with hydrogen bromide -Trifluoro-phenyl) -methanone (Example 81),
[177] ggg) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3,5-di with hydrogen bromide Fluoro-phenyl) -methanone (Example 82),
[178] hhh) 4-amino-2- {4- [4- (2-methoxy-ethyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(with hydrogen bromide)-( 3-fluoro-4-methoxy-phenyl) -methanone (Example 83),
[179] iii) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(4-fluoro- with hydrogen bromide 3-methoxy-phenyl) -methanone (Example 84),
[180] jjj) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-fluoro- with hydrogen bromide 4-methoxy-phenyl) -methanone (Example 85),
[181] kkk) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(4-difluoromembrane with hydrogen bromide Oxy-phenyl) -methanone (Example 86),
[182] lll) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-trifluorome, with hydrogen bromide Oxy-phenyl) -methanone (Example 87),
[183] mmm) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(4-piperidine with hydrogen bromide -1-yl-phenyl) -methanone (Example 88),
[184] nnn) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(4-morpholine-) with hydrogen bromide 4-yl-phenyl) -methanone (Example 89),
[185] ooo) (4-amino-2- {4- [4- (2-methoxy-ethyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-with hydrogen bromide (3,5-difluoro-phenyl) -methanone (Example 90),
[186] ppp) (4-amino-2- {4- [4- (2-methoxy-ethyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-with hydrogen bromide (3,4-difluoro-phenyl) -methanone (Example 91),
[187] qqq) (4-amino-2- {4- [4- (2-methoxy-ethyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-with hydrogen bromide (3-methoxy-phenyl) -methanone (Example 92),
[188] rrr) (4-amino-2- {4- [4- (2-methoxy-ethyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-with hydrogen bromide Benzo [1,3] dioxol-5-yl-methanone (Example 93),
[189] sss) 4-amino-2- {4- [4- (2-methoxy-ethyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(with hydrogen bromide 2,3-dihydro-benzo [1,4] dioxin-6-yl) -methanone (Example 94),
[190] ttt) {4-amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3,5-difluoro) with hydrogen bromide Rho-phenyl) -methanone (Example 95),
[191] uuu) {4-amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3,5-difluoro) with hydrogen bromide Ro-4-methoxy-phenyl) -methanone (Example 96),
[192] vvv) {4-amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(4-fluoro-3) with hydrogen bromide -Methoxy-phenyl) -methanone (Example 97),
[193] www) {4-amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-trifluoromethoxy with hydrogen bromide -Phenyl) -methanone (Example 98),
[194] xxx) (4-amino-2- {4- [4- (2-hydroxy-ethyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3 with acetic acid -Fluoro-phenyl) -methanone (Example 99),
[195] yyy) [4-amino-2- (4-piperazin-1-yl-phenylamino) -thiazol-5-yl]-(3,4,5-trifluoro-phenyl) -meta with acetic acid Rice field (Example 100),
[196] zzz) [4-amino-2- (4-piperazin-1-yl-phenylamino) -thiazol-5-yl]-(3,5-difluoro-4-methoxy-phenyl with acetic acid ) -Methanone (Example 101),
[197] aaaa) [4-Amino-2- (4-piperazin-1-yl-phenylamino) -thiazol-5-yl]-(4-fluoro-3-methoxy-phenyl) -meta with acetic acid Rice field (Example 102),
[198] bbbb) [4-amino-2- (4-piperazin-1-yl-phenylamino) -thiazol-5-yl]-(3-trifluoromethoxy-phenyl) -methanone with acetic acid Example 103),
[199] cccc) {4-Amino-2- [4- (4-sec-butyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-fluoro-phenyl) -methanone (Example 104),
[200] dddd) {4-amino-2- [4- (4-sec-butyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl} -benzo [1,3] dioxol-5- Mono-methanone (Example 105),
[201] eeee) {4-amino-2- [4- (4-sec-butyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(2,3-dihydro-benzo [1 , 4] dioxin-6-yl) -methanone (Example 106),
[202] ffff) {4-amino-2- [4- (4-cyclopentyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl} -benzo [1,3] dioxol-5-yl -Methanone (Example 107),
[203] gggg) {4-amino-2- [4- (4-cyclopentyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(2,3-dihydro-benzo [1, 4] dioxin-6-yl) -methanone (Example 108),
[204] hhhh) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-trifluoromethoxy-phenyl) -meta Rice field (Example 109),
[205] iiii) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-fluoro-phenyl) -methanone ( Example 110),
[206] jjjj) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-difluoromethoxy-phenyl) -meta Rice field (Example 111),
[207] kkkk) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-hydroxy-phenyl) with acetic acid Methanon, (Example 112),
[208] llll) {4-amino-2- [4- (4-isobutyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-hydroxy-phenyl) with acetic acid -Methanone (Example 113),
[209] mmmm) 4-amino-2- (4-piperazin-1-yl-phenylamino) -thiazol-5-yl]-(3-difluoromethoxy-phenyl) -methanone (Example 114),
[210] nnnn) {4-amino-2- [4- (4-cyclopropylmethyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-difluoromethoxy-phenyl)- Metanon (Example 115),
[211] oooo) {4-amino-2- [4- (4-cyclopropylmethyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-methoxy-phenyl) -methanone (Example 116),
[212] pppp) {4-amino-2- [4- (4-cyclopropylmethyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl} -benzo [1,3] dioxol-5- Mono-methanone (Example 117),
[213] qqqq) {4-amino-2- [4- (4-cyclopropylmethyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(2,3-dihydro-benzo [1 , 4] dioxin-6-yl) -methanone (Example 118),
[214] rrrr) {4-amino-2- [4- (4-cyclopropylmethyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-fluoro-4-hydroxy- Phenyl) -methanone (Example 119),
[215] ssss) {4-amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-difluoromethoxy-phenyl) -methanone (Example 120),
[216] tttt) {4-amino-2- [4- (4-cyclopropylmethyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-fluoro-phenyl) -methanone (Example 121),
[217] uuuu) {4-Amino-2- [4- (4-cyclopropylmethyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-fluoro-4-methoxy- Phenyl) -methanone (Example 122),
[218] vvvv) {4-amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-hydroxy-phenyl) -methanone (implemented 123), and
[219] wwww) 3- {4-Amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazole-5-carbonyl} -benzonitrile (Example 124).
[220] Compounds disclosed herein and included in Formula I may exhibit tautomerization or structural isomerization. The present invention is intended to include any tautomeric or structural isomeric forms of these compounds, or mixtures of these forms, and is not intended to be limited to any one tautomeric or structural isomeric form described in the above formula.
[221] The compounds of the present invention can be prepared by any conventional means. Suitable methods for synthesizing these compounds are provided in the Examples. Typically, the compounds of formula (I) can be prepared according to one of the synthetic routes described below.
[222] The compounds of the present invention can be prepared by alkylation and cyclization of a number of thiourea derivatives, as shown in Scheme I, using known reactions. Among thiourea derivatives that can be used, nitroamidinothiourea (Binu, R. et al . , Org. Prep. Proced. Int. 1998, 30, 93-96); 1-[(arylthiocarbamoyl) amino] -3,5-dimethylpyrazole (Jenardanan, GC et al . Synth. Commun . 1997, 27, 3457-3346); And N- (aminoiminomethyl) -N'-phenylthiourea (Rajasekharan, KN et al. Synthesis 1986, 353-355).
[223]
[224] Other thiourea derivatives that can be used for preparing the compounds of the present invention by alkylation and cyclization are N- cyanothioureas (Gewald, K. et al. , J. Prakt. Chem. 1967, 97-104). For example, according to Scheme IA, N-cyanothiourea of Formula 4A is reacted with a halomethylketone such as bromomethylketone of Formula 5 at a temperature of about room temperature to about 65 ° C. Can be obtained.
[225]
[226] Alternatively, the compounds of the present invention may also be used in the reaction of a resin-bonded substituted (aminothioxomethyl) carbamididothio acid methyl ester of formula (8) with a bromomethyl aryl ketone of formula (5) as shown in Scheme II below. It is conveniently manufactured by
[227]
[228] Resin-bound thiourea derivatives of Formula 8 may be made by any conventional procedure known to organic synthesis practitioners. For example, in an inert solvent such as a polar aprotic solvent (eg N, N-dimethylformamide) of a base such as tertiary amine (eg triethylamine or diisopropylethylamine) It can be conveniently prepared by the reaction of the resin bound thiouronium salt of formula (7) with isothiocyanate of formula (2) in the presence. The reaction is conveniently carried out at a temperature of approximately room temperature. The resin-bound thiourea derivative of formula 8 is then subjected to halomethylketone (eg, in a suitable inert solvent such as a polar aprotic solvent (eg N, N-dimethylformamide) at approximately room temperature. Bromomethylketone of formula 5) to convert to the product of formula 6.
[229] Nucleophilic Aromatic Substitution
[230]
[231] As shown in Scheme III, R ³ represents nitro and R ⁴ is selected from NR ²⁰ R ²¹ , wherein R ²⁰ and R ²¹ are independently lower alkyl, which may itself be substituted by OH, and H Or alternatively NR ²⁰ R ²¹ may optionally form a ring having 5 to 6 atoms, which ring may comprise one or more additional heteroatoms, and may itself be substituted with OH , And optionally substituted by OH, wherein R ⁵ represents hydrogen (ie, a compound of formula 10) is an inert solvent such as a lower alcohol (eg, ethanol, isopropanol, N-butanol And the like) or in the presence of an additional base such as a tertiary amine (eg triethylamine or diisopropylethylamine) in a polar aprotic solvent (eg N, N-dimethylformamide) To a compound of formula 9 It can be conveniently prepared by treating with an amine of HNR ²¹ R ²⁰ . The reaction is conveniently carried out at a temperature of about 70 degrees to about 110 degrees, preferably at about reflux of the solvent or about 100 degrees, or lower.
[232] C. Removal of Protectors
[233]
[234] As shown in Scheme IV, a compound of Formula I wherein R ¹ represents H (ie, a compound of Formula 11) is derived from a compound of Formula 30, wherein R ³⁰ represents a protecting group commonly used for the protection of secondary amines. It can be prepared by removing the protecting group. Many such protecting groups are known to organic synthesisers. For example, some suitable protecting groups are listed in "Protective Groups in Organic Synthesis" (Greene, TW and Wuts, PGM, 2nd Edition, John Wiley & Sons, NY1991). Preferred protecting groups are those compatible with the reaction conditions used to prepare the compounds of the invention. One such protecting group is a tert-butoxycarbonyl (t-BOC) group. tert-butoxy carbonyl group, R <^30> is The compound of formula (30), wherein R ¹ represents H, can be conveniently removed by treatment with an acid to obtain a compound of formula (I). Examples of acids that can be used to achieve the above conversion are well known and include trifluoroacetic acid, hydrochloric acid, hydrofluoric acid, and aluminum chloride. For example, a t-BOC group, R ³⁰ is Compounds of formula (30) which can be conveniently removed by treatment with trifluoroacetic acid in an inert solvent such as a halogenated hydrocarbon (eg dichloromethane). The reaction can be carried out at a temperature of about 0 degrees to about room temperature, preferably at about room temperature.
[235] D. Separation of the mixture of stereoisomers into optically pure stereoisomers (if the compound of formula I is chiral)
[236] Optical separation of the isomeric structures of formula (I) can be carried out according to known methods such as, for example, resolution or chiral high pressure liquid chromatography (also known as chiral HPLC). Partitioning methods are well known and are summarized in "Enantiomers, Racemates, and Resolutions" (Jacques, J. et al., John Wiley and Sons, NY, 1981). Methods for chiral HPLC are also well known and are described in "Separation of Enantiomers by Liquid Chromatographic Methods" (Pirkle, WH and Finn, J., "Asymmetric Synthesis", Vol. 1, Morrison, JD, Ed., Academic Press , Inc., NY 1983, pp. 87-124).
[237] E. Conversion of a Compound of Formula (I) with Basic Nitrogen into a Pharmaceutically Acceptable Acid Addition Salt
[238] Any conversion of the compound of formula (I) with basic nitrogen into a pharmaceutically acceptable acid addition salt can be accomplished by conventional means. For example, the compounds can be treated with inorganic acids such as, for example, hydrochloric acid, hydrobromic acid, sulfuric acid, nitric acid, phosphoric acid or with appropriate organic acids such as acetic acid, citric acid, tartaric acid, methanesulfonic acid, p-toluenesulfonic acid and the like.
[239] F. Conversion of Compounds of Formula (I) Having Carboxylic Acid Groups into Pharmaceutically Acceptable Alkali Metal Salts
[240] Any conversion of the compound of formula (I) having a carboxylic acid group into a pharmaceutically acceptable alkali metal salt can be achieved by conventional means. For example, the compound may be treated with an inorganic base such as lithium hydroxide, sodium hydroxide, potassium hydroxide and the like.
[241] G. Conversion of a Compound of Formula (I) Having a Carboxylic Acid Group to a Pharmaceutically Acceptable Ester
[242] Any conversion of the compound of formula (I) having carboxylic acid groups into pharmaceutically acceptable esters can be accomplished by conventional means. Conditions for ester formation will depend on the stability of other functional groups in the molecule with respect to the reaction conditions. If other parts of the molecule are stable to acidic conditions, the ester can be conveniently prepared by heating in a solution of an inorganic acid (eg sulfuric acid) in alcohol. Other methods of preparing esters that may be convenient if the molecule is not stable to acidic conditions include treating the compound with an alcohol in the presence of a coupling agent and in the presence of additional agents that can accelerate the reaction. do. Many such coupling agents are known to organic chemists. Two examples are dicyclohexylcarbodiimide and triphenylphosphine / diethyl azodicarboxylate. When dicyclohexylcarbodiimide is used as the coupling agent, the reaction is carried out in an inert solvent such as a halogenated hydrocarbon (eg dichloromethane) at a temperature of about 0 degrees to about room temperature, preferably at about room temperature. It is conveniently carried out by treatment with clohexylcarbodiimide and optionally present catalytic amounts (0-10 mol%) of N, N-dimethylaminopyridine. When triphenylphosphine / diethyl azodicarboxylate is used as the coupling agent, the reaction is carried out at ether (eg tetrahydrofuran) or aromatic hydrocarbon (at a temperature of about 0 degrees to about room temperature, preferably at about 0 degrees. In an inert solvent such as, for example, benzene), the acid is conveniently carried out by treating the acid with alcohol, triphenylphosphine and diethyl azodicarboxylate.
[243] Returning to the intermediate, the isothiocyanate intermediate of formula (2) used to make the compounds of the present invention can be made by any conventional means. For example, they can be made by the route shown in Scheme V below.
[244]
[245] A number of methods familiar to those skilled in the art can be used to reduce the nitro group in the compound of formula 12 to obtain aniline of formula 13. Such methods include (1) treating the nitro compound of formula 12 with iron / acetic acid, tin (II) chloride / hydrochloric acid, or zinc and ammonium chloride; And (2) hydrogenation in the presence of a noble metal catalyst such as palladium on carbon.
[246] Isothiocyanates of formula (2) can be made from anilines of formula (13) using any one of a number of reagents known to organic synthesisers, which would be useful for converting aniline of formula (13) to isothiocyanate of formula (2). Can be. Among these reagents are carbon disulfide, thiophosphene, 1,1'-thiocarbonylbis (2-pyridone), and thiocarbonyl diimidazole. The reaction is carried out in a suitable inert solvent such as a polar aprotic solvent (eg N, N-dimethylformamide) at a temperature of about -20 degrees to about 0 degrees, preferably at about -15 degrees. It can be carried out by treatment with thiocarbonyl diimidazole.
[247] The nitro compound of formula 12 may be made by a variety of methods known in the art of organic synthesis. For example, they can be made by nucleophilic substitution of nitrobenzene derivatives having leaving groups in the para position relative to nitro groups according to Scheme VI below.
[248]
[249] The nucleophilic substitution reaction between the amine of formula 15 and nitrobenzene of formula 14, wherein X is a leaving group, to obtain a substitution product of formula 12 is carried out at a temperature of about 50 to about 100 degrees, preferably about 80 degrees. In the presence of any inert solvent, such as acetonitrile, this can be done conveniently by heating these materials together (Scheme VI). Suitable leaving groups of formula (X) include chloride and fluoride. By way of example, and not by way of limitation, the following compounds of formula 14 are commercially available:
[250]
[251] Alternatively, the nitro compound of formula 12 can be synthesized according to Scheme VII below.
[252]
[253] The alkylation of the compound of formula 16 to obtain a compound of formula 12, wherein R ¹ represents an alkyl group, is carried out in an inert solvent such as acetonitrile, lower alkyl ketones (eg acetone or 2-butanone), polar aprotic solvents (eg N, N-dimethylformamide), or an aromatic hydrocarbon (such as benzene or toluene), in the presence of a base such as sodium carbonate or potassium carbonate, and in the presence of a catalyst such as potassium iodide, the compound is an alkylating agent of formula 17 Wherein X is one of a number of leaving groups that are well known to those skilled in the art of organic synthesis, such as halides (e.g. chloride, bromide, iodide) or sulfonates (e.g. mesylate, Tosylate, triflate) and the like). Except where the leaving group is triflate, the reaction may conveniently be carried out at a temperature of about 50 degrees to about 110 degrees, preferably about 80 degrees. If the leaving group is triflate, the reaction is conveniently carried out at a temperature of about 0 degrees to room temperature, preferably at about room temperature.
[254] Compounds of formula 12 wherein R ¹ represents an acyl group can be prepared by acylation of compounds of formula 16. The reaction can be accomplished using known methods. For example, a compound of Formula 16 may be used in the presence of a coupling agent (many examples of which are well known per se in peptide chemistry) and materials that increase the reaction rate, such as 1-hydroxybenzotriazole or 1- In the presence of any of hydroxy-7-azabenzotriazole, it may be treated with a carboxylic acid of formula 17, wherein X represents OH; Or by reacting a compound of formula 16 with a reactive derivative of a carboxylic acid, such as a corresponding acid halide (eg, acid chloride), acid anhydride, mixed anhydride, activated ester, and the like. The reaction is carried out in an inert solvent such as a halogenated hydrocarbon (eg dichloromethane) in the presence of a base such as pyridine or a tertiary amine (eg diisopropylethylamine) or an inorganic base such as sodium carbonate or potassium carbonate. Conveniently performed by treating the compound of Formula 16 with acid chloride at a temperature from about room temperature to about room temperature, preferably about room temperature.
[255] The compounds of formula (12) is R ¹ representing an alkoxy group is, and by alkoxy carbonylation of a compound of formula 16 R ¹ is an alkoxycarbonyl group may be prepared by obtaining the compound of formula 12. Such synthesis can be accomplished using reactions well known in the field of organic chemistry and peptide chemistry. Conditions for many of these reactions are given in "Protective Groups in Organic Synthesis" (Greene, TW and Wuts, PGM, 2nd Edition, John Wiley & Sons, NY 1991). In the case of preferred alkoxycarbonyl protecting groups, ie tert-butoxycarbonyl (t-BOC) groups, the protecting group is an inert solvent such as a mixture of ether and water such as dioxane, or a halogenated solvent (such as dichloromethane), or acetonitrile Among them, it can be introduced by reacting an amine of formula 16 with di-tert-butyl dicarbonate in the presence of a base or a catalyst such as N, N-dimethylamino-pyridine. As an alternative to di-tert-butyl dicarbonate, the following reagents may also be used: 2- (tert-butoxycarbonyloxymino) -2-phenylacetonitrile, or tert-butyl azidoformate.
[256] R ¹ represents a compound of formula (12) is a sulfonyl group, the R ¹ to biotinylated sulfonate a compound of formula 16 may be prepared by obtaining the compound of formula (12) represents a sulfonyl group. Such synthesis can be accomplished using reactions well known in the organic chemistry art. For example, compounds of formula 12, wherein R ¹ represents a sulfonyl group, can be prepared by reacting a compound of formula 16 with sulfonyl chloride in the presence of a suitable base such as, for example, pyridine, which may also be used as a solvent. The reaction can also be carried out by using a tertiary amine as the base in the presence of an inert solvent such as tetrahydrofuran or dichloro-methane; Or by using an alkali metal hydroxide such as sodium hydroxide as the base in an aqueous solution. The reaction is conveniently carried out at a temperature of about room temperature to about 80 degrees, preferably at about room temperature.
[257] Exemplary compounds of formula (16) can be obtained as shown below.
[258]
[259] Bromomethylketone intermediate 5 used to make the compounds of the invention is commercially available or can be made using one of a number of methods known to organic synthesisers, for example: arene and bromoacetyl bromide Or Friedel-Crafts reaction with bromoacetyl chloride; Oxidation of 2-bromo-1-phenethyl alcohol; Reaction of diazomethyl ketone with HBr; Reduction of dibromomethyl ketone (see Scheme VIII) below; Or reaction of methyl ketone with a brominating agent (see Scheme IX) such as bromine, copper (II) bromide, tetrabutylammonium tribromide, or 5,5-dibromo-barbituric acid.
[260] According to the method of Diwu et al. (Tetrahedron Lett. 1998, 39, 4987-4990), the methyl ketone of formula 19 can be converted to the corresponding dibromomethyl ketone of formula 20 by treatment with pure sulfuric acid in bromine. . Thereafter, the dibromomethyl ketone of the formula (20) can be converted to the target bromomethyl ketone of the formula (5) by reduction with diethyl phosphite.
[261]
[262] Bromomethyl ketones of formula 5 may also be prepared directly from methyl ketones of formula 19 using a variety of reagents well known to those skilled in the art of organic synthesis, such as those described above. For example, the reaction may be carried out in a suitable inert solvent such as halogenated hydrocarbons (eg carbon tetrachloride) in the presence of other agents that facilitate the reaction, such as Bronsted or Lewis acid catalysts (eg aluminum chloride or acetic acid). It may conveniently be carried out by treating 19 methyl ketones with bromine. The optimum reaction temperature depends on whether or not the catalyst is to be used. If aluminum chloride is used, the reaction is conveniently carried out at about 0 degrees. When acetic acid is added or no catalyst is used, the reaction is conveniently carried out at a temperature of about room temperature to about 80 degrees, preferably at about room temperature. Alternatively, the methyl ketone of formula 19 can be converted to bromomethylketone of formula 5 by treatment with copper (II) bromide, preferably at reflux temperature, in a suitable non-reactive solvent such as ethyl acetate.
[263]
[264] Composition / Formulation
[265] In an alternative embodiment, the present invention is directed to a pharmaceutical composition containing one or more compounds of formula (I), or pharmaceutically acceptable salts or esters thereof.
[266] The pharmaceutical composition can be administered orally, for example in the form of tablets, coated tablets, dragees, hard or soft gelatin capsules, solutions, emulsions, or suspensions. They can also be administered rectally, for example in the form of suppositories, or parenterally in the form of injections.
[267] Pharmaceutical compositions of the invention containing a compound of formula (I), and / or salts or esters thereof, are formulated in the art, such as conventional mixing, encapsulating, dissolving, granulating, emulsifying, trapping, glycosylating, or freezing. It can be produced by means of the oxidization process. These pharmaceutical preparations can be formulated with therapeutically inert, inorganic or organic carriers. Lactose, corn starch or derivatives thereof, talc, stearic acid or salts thereof can be used as carriers for such tablets, coated tablets, dragees and hard gelatin capsules. Suitable carriers for soft gelatin capsules include vegetable oils, waxes and fats. Depending on the nature of the active substance, no carriers are usually required at all for soft gelatin capsules. Suitable carriers for the preparation of solutions and syrups include water, polyols, saccharose, invert sugar and glucose. Suitable carriers for injection include water, alcohols, polyols, glycerin, vegetable oils, phospholipids and surfactants. Suitable carriers for suppositories include natural or hardened oils, waxes, fats and semi-liquid polyols.
[268] Pharmaceutical formulations may also contain preservatives, solubilizers, stabilizers, wetting agents, emulsifiers, sweeteners, colorants, flavoring agents, salts for varying the osmotic pressure, buffers, coatings or antioxidants. In addition, they may contain other therapeutically valuable substances comprising additional active ingredients other than those of formula (I).
[269] As mentioned above, the compounds of the present invention comprising compounds of formula I are useful for the treatment or control of cell proliferative disorders, in particular oncological disorders. These compounds, and formulations containing the compounds, are particularly useful for the treatment or control of solid tumors such as, for example, breast, colon, lung, and prostate tumors.
[270] By therapeutically effective amount of a compound according to the invention is meant an amount of the compound effective to prevent, alleviate or ameliorate the symptoms of a disease or prolong the survival of a treated subject. Determination of a therapeutically effective amount is within the skill of the art.
[271] The therapeutically effective amount or dosage of a compound according to the invention can vary within wide ranges and can be determined in a manner known in the art. The dosage will be adjusted for the individual requirements in each specific case, including the particular compound (s) being administered, the route of administration, the condition to be treated, and the patient to be treated. Typically, for oral or parenteral administration to adult humans weighing approximately 70 Kg, about 10 mg to about 10,000 mg, preferably about 200 mg to about 1,000 mg, although the upper limit may be exceeded if indicated. The daily dosage of will be appropriate. The daily dose may be administered in a single dose or in divided doses, or in the case of parenteral administration, may be infused continuously.
[272] In another embodiment, the present invention also relates to novel intermediates useful for the preparation of compounds of formula (I). These novel intermediates include the following compounds:
[273] a) 1- (2-fluoro-4-isothiocyanatophenyl) -4-methylpiperazine (Example 2C),
[274] b) 1- (1-methylethyl) -4- (4-nitrophenylpiperazine) (Example 3A),
[275] c) 4- [4- (1-methylethyl) -1-piperazinyl] benzeneamine (Example 3B),
[276] d) 1- (4-isothiocyanatophenyl) -4- (1-methylethyl) piperazine (Example 3C),
[277] e) 4- (2-hydroxyethyl) -1- (4-isothiocyanatophenyl) piperazine (Example 5B),
[278] f) 1- (4-isothiocyanatophenyl) -4- (2-methoxy-ethyl) piperazine (Example 14B),
[279] g) 1- (4-isothiocyanatophenyl) -4- (1-methylpropyl) piperazine (Example 14C),
[280] h) 4-cyclopentyl-1- (4-isothiocyanatophenyl) piperazine (Example 14D),
[281] i) 1- (4-isothiocyanatophenyl) -4- (2-methylpropyl) piperazine (Example 14E),
[282] j) 4- (3-hydroxypropyl) -1- (4-isothiocyanatophenyl) piperazine (Example 14F),
[283] k) 2-bromo-1- (3,4,5-trifluoro-phenyl) ethanone (Example 14H),
[284] 1) 2-bromo-1- (4-fluoro-3-methoxy-phenyl) ethanone (Example 14I),
[285] m) 2-bromo-1- (4-fluoro-3-methoxy-phenyl) ethanone (Example 14K),
[286] n) 2-bromo-1- (3-difluoromethoxy-phenyl) ethanone (Example 14L),
[287] o) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (4-chloro-3-nitrophenyl) methanone (Example 15 ),
[288] p) carbamimidothioic acid [[4- (4-methyl-1-piperazinyl) phenylamino] thioxomethyl] bound to a polymer (Example 18),
[289] q) carbamimidothioic acid [[3-fluoro-4- (4-methyl-1-piperazinyl) phenylamino] thioxomethyl], bound to a polymer (Example 19),
[290] r) carbamimidothioic acid [[4- [4- (1-methylethyl) -1-piperazinyl] phenylamino] thioxomethyl], bound to a polymer] (Example 20),
[291] s) carbamimidothioic acid [[4- (4-acetyl-1-piperazinyl) phenylamino] thioxomethyl] bound to a polymer (Example 21),
[292] t) carbamimidothioic acid [[4- [4- (2-hydroxyethyl) -1-piperazinyl] phenylamino] thioxomethyl] bound to a polymer (Example 22),
[293] u) carbamimidothioic acid [[4- [4-[(1,1-dimethylethoxy) carbonyl] -1-piperazinyl] phenylamino] thioxomethyl] bound to a polymer (Example 23A) ),
[294] v) carbamimidothioic acid [[4- [4- (2-methoxyethyl) -1-piperazinyl] phenylamino] thioxomethyl], bound to a polymer (Example 23B),
[295] w) carbamimidothioic acid [[4- [4- (1-methylpropyl) -1-piperazinyl] phenylamino] thioxomethyl], bound to a polymer (Example 23C),
[296] x) carbamimidothioic acid [[4- (4-cyclopentyl-1-piperazinyl) phenylamino] thioxomethyl], bound to a polymer (Example 23D),
[297] y) carbamimidothioic acid [[4- [4- (2-methylpropyl) -1-piperazinyl] phenylamino] thioxomethyl] bound to a polymer] (Example 23E),
[298] z) carbamimidothioic acid [[4- [4- (3-hydroxypropyl) -1-piperazinyl] phenylamino] thioxomethyl], bound to a polymer (Example 23F),
[299] aa) 4- [4-[[4-amino-5- (3-fluorobenzoyl) -2-thiazolyl] amino] phenyl] -1-piperazinecarboxylic acid, 1,1-dimethylethyl ester 54A),
[300] bb) 4- [4-[[4-amino-5- [4- (1-pyrrolidinyl) benzoyl] -2-thiazolyl] amino] phenyl] -1-piperazinecarboxylic acid, 1,1- Dimethylethyl ester (Example 55A),
[301] cc) 4- [4-[[4-amino-5- (3-fluoro-4-methoxybenzoyl) -2-thiazolyl] amino] phenyl] -1-piperazinecarboxylic acid, 1,1- Dimethylethyl ester (Example 56A).
[302] The following examples illustrate preferred methods of synthesis and formulations of the compounds of the present invention.
[303] Example 1
[304] 1- (4-isothiocyanatophenyl) -4-methylpiperazine
[305]
[306] A mixture of thiocarbonyldiimidazole (4.46 g, 25.0 mmol) (Aldrich) and N, N-dimethylformamide (20 mL) was cooled to about -15 ° C and 4- (4-methyl-1-pipera Genyl) benzeneamine solution (prepared according to the method of WO 9921845 of Chong, WK et al .; 4.78 g, 25.0 mmol) was added over 30 minutes. The cooling bath was removed and the mixture was stirred for 1 hour. The mixture was cooled to 0 ° C. and ice water was added. After 30 minutes, the mixture was extracted with ether (3 × 200 mL), dried (Na ₂ SO ₄ ), filtered and evaporated to give 1- (4-isothiocyanatophenyl) -4-methylpiperazine pale purple Obtained as a solid (4.84 g, 83%).
[307] Example 2
[308] 1- (2-fluoro-4-isothiocyanatophenyl) -4-methylpiperazine
[309]
[310] A. 1- (2-fluoro-4-nitrophenyl) -4-methylpiperazine
[311] A solution of acetonitrile (150 mL) of 3,4-difluoronitrobenzene (12.00 g, 75.4 mmol) (Aldrich) and N-methylpiperazine (18.89 g, 188.6 mmol) was heated to reflux for 3 hours. The reaction mixture was left at room temperature overnight, then the solvent was evaporated under reduced pressure, the residue was partitioned between water and ethyl acetate (200 mL each), the aqueous layer was extracted with ethyl acetate (3 x 200 mL), and the organic layer was extracted. Combined, washed with water and brine (200 mL each), dried (MgSO ₄ ), filtered and evaporated under reduced pressure to afford 1- (2-fluoro-4-nitrophenyl) -4-methylpiperazine as a light brown solid. (17.5 g, 97%, mp 68-70 ° C.)
[312] B. 3-fluoro-4- (4-methyl-1-piperazinyl) benzeneamine
[313] 1- (2-fluoro-4-nitrophenyl) -4-methylpiperazine (5.00 g, 20.9 mmol) (from step A above) and 10% palladium / carbon (0.20 g, 0.2 mmol) ethyl acetate (100 mL) and ethanol (50 mL) solutions were hydrogenated at 50 psi for 4 hours in a Parr shaker. The mixture was filtered through Celite ^™ . Celite ^™ was washed with ethyl acetate (50 mL) and the solvent was evaporated to give 3-fluoro-4- (4-methyl-1-piperazinyl) benzeneamine as a white solid (4.05 g, 93% , mp 89-91 ° C).
[314] C. 1- (2-Fluoro-4-isothiocyanatophenyl) -4-methylpiperazine
[315] Thiocarbonyl diimidazole (3.00 g, 3.53 mmol) (Aldrich) was dissolved in N, N-dimethylformamide (10 mL) and the solution was cooled to -10 ° C (ice / acetone bath). A solution of N, N-dimethylformamide (30 mL) of 3-fluoro-4- (4-methyl-1-piperazinyl) benzeneamine (2.26 g, 10.8 mmol) (from step B above) was added in 30 minutes. After addition over, the cooling bath was removed and the solution was stirred overnight. To the mixture was added ice water (100 mL) and extracted with ether (3 x 200 mL). The combined ether layers were dried (MgSO ₄ ), filtered and evaporated to give 1- (2-fluoro-4-isothio-cyanatophenyl) -4-methylpiperazine as a yellow oil which solidified upon standing. (3.88 g, 92%).
[316] Example 3
[317] 1- (4-isothiocyanatophenyl) -4- (1-methylethyl) piperazine
[318]
[319] A. 1- (1-methylethyl) -4- (4-nitrophenyl) piperazine)
[320] 1- (4-nitrophenyl) piperazine (2.00 g, 9.7 mmol) (Acros Organics), powdered potassium carbonate (2.7 g, 19.3 mmol), 2-bromopropane (0.96 mL, 10.1 mmol), potassium iodide (50 mg) and a catalytic amount of 18-crown-6 in acetonitrile (15 mL) were heated to reflux overnight. The mixture was filtered and the filter cake was washed with acetonitrile. The solvent was evaporated from the combined filtrates and the residue was partitioned between water and ethyl acetate. The layers were separated and the aqueous layer was extracted twice with ethyl acetate. The combined organic layers were washed with brine, dried (MgSO ₄ ), filtered, evaporated and chromatographed (0 to 66% acetone / dichloromethane) to give 1- (1-methylethyl) -4- (4-nitrophenylpiperazine ) Was obtained (1.7 g, 71%).
[321] B. 4- [4- (1-methylethyl) -1-piperazinyl] benzeneamine
[322] A mixture of 1- (1-methylethyl) -4- (4-nitrophenylpiperazine) (1.7 g, 6.8 mmol) (from step A above) and ethanol (30 mL) of 10% palladium / activated carbon was cooled to room temperature and atmospheric pressure. Hydrogenated overnight. The catalyst was filtered off and the filter cake was washed thoroughly with ethanol. The mixture was evaporated under reduced pressure to afford 4- [4- (1-methylethyl) -1-piperazinyl] benzeneamine as dark brown oil (1.6 g, quantitative yield).
[323] C. 1- (4-isothiocyanatophenyl) -4- (1-methylethyl) piperazine
[324] Cool the solution of 4- [4- (1-methylethyl) -1-piperazinyl] benzeneamine (1.6 g, 6.8 mmol) (from step B above) in N, N-dimethylformamide (25 mL) To a solution of (-15 ° C.) thiocarbonyldiimidazole (1.4 g, 7.7 mmol) (Aldrich) in N, N-dimethylformamide (30 mL) was added dropwise over 20 minutes. After the addition was completed, the mixture was stirred at -15 ° C for 20 minutes, then the cooling bath was removed and the solution was stirred for 1 hour. Ice water was added and the mixture was extracted with ether. The ether layer was dried (MgSO ₄ ), filtered and evaporated to give 1- (4-isothiocyanatophenyl) -4- (1-methylethyl) piperazine (1.8 g, quantitative yield).
[325] Example 4
[326] 1- (acetyl) -4- (4-isothiocyanatophenyl) piperazine
[327]
[328] This compound can be prepared by the method of Chong, W. K. et al. (WO 9921845) or the following.
[329] A. 1-acetyl-4- (4-nitrophenyl) piperazine
[330] Dichloromethane (75 mL) of powdered potassium carbonate (276 mg, 2 mmol) and acetyl chloride (0.14 mL, 2 mmol) in 1- (4-nitrophenyl) piperazine (2.1 g, 1 mmol) (Acros Organics) ) In solution. The mixture was stirred overnight at room temperature, followed by further addition of powdered potassium carbonate (500 mg, 3.6 mmol) and acetyl chloride (0.2 mL, 2.8 mmol). The mixture was stirred at rt overnight, then filtered and the filter cake washed with dichloromethane. The solvent was evaporated from the filtrate and the residue was chromatographed (0-66% acetone / dichloromethane) to give 1-acetyl-4- (4-nitrophenyl) piperazine (1.8 g, 72%).
[331] B. 1-acetyl-4- (4-aminophenyl) piperazine
[332] A mixture of 1-acetyl-4- (4-nitrophenyl) piperazine (1.8 g, 7.2 mmol) (from step A above) and 10% palladium / activated carbon in ethanol (50 mL) was hydrogenated overnight at room temperature and atmospheric pressure. . The catalyst was filtered off and the filter cake was washed thoroughly with ethanol. The mixture was evaporated under reduced pressure and 1-acetyl-4- (4-amino-phenyl) piperazine was obtained as a grayish white solid (1.0 g, 63%).
[333] C. 1- (acetyl) -4- (4-isothiocyanatophenyl) piperazine
[334] A solution of 1-acetyl-4- (4-aminophenyl) piperazine (1.0 g, 4.6 mmol) (from step B above) in N, N-dimethylformamide (10 mL) was cooled (-15 ° C.) Carbonyldiimidazole (855 mg, 4.8 mmol) was added dropwise to a solution in N, N-dimethylformamide (10 mL). After the addition was completed, the mixture was stirred at -15 ° C for 30 minutes, then the cooling bath was removed and the solution was stirred for 1 hour. Ice water was added and the mixture was extracted with ether (4 x 50 mL). The combined ether layers were washed with brine, dried (MgSO ₄ ), filtered and evaporated to afford 1- (acetyl) -4- (4-isothiocyanatophenyl) piperazine (1.0 g, 84%).
[335] Example 5
[336] 4- (2-hydroxyethyl) -1- (4-isothiocyanatophenyl) piperazine
[337]
[338] This compound was prepared from 4- (4-nitrophenyl) -1-piperazine-ethanol (Bionet Research Ltd.) using the hydrogenation reaction and isothiocyanate formation reaction used in Example 4.
[339] Example 6
[340] 4- (4-isothiocyanatophenyl) -1-piperazinecarboxylic acid, 1,1-dimethylethyl ester
[341]
[342] A. 4- (4-nitrophenyl) -1-piperazinecarboxylic acid, 1,1-dimethylethyl ester
[343] 1- (4-nitrophenyl) piperazine (20 g, 96.5 mmol) (Acros Organics) was dissolved in dioxane (300 mL) and diisopropylethylamine (13.7 g, 106 mmol) (Aldrich) was added. . To this solution was added di-tert-butyldicarbonate (21 g, 96.6 mmol). After stirring overnight, the mixture was poured into water (1 L) and stirred for 10 minutes. The aqueous layer was extracted with ethyl acetate (2 x 500 mL) and the combined organic extracts were dried (MgSO ₄ ), filtered and evaporated in vacuo. The residue was recrystallized from an ethyl acetate / hexane mixture to give 4- (4-nitrophenyl) -1-piperazinecarboxylic acid, 1,1-dimethylethyl ester (22.7 g, 77%).
[344] B. 4- (4-aminophenyl) -1-piperazinecarboxylic acid, 1,1-dimethylethyl ester
[345] 4- (4-nitrophenyl) -1-piperazinecarboxylic acid, 1,1-dimethylethyl ester (20 g, 65 mmol) is dissolved in anhydrous ethanol (250 mL) and 10% Pd / C (1.8 g) ) Was added. The mixture was stirred under 10 psi of hydrogen for 1.5 hours and filtered through a Celite pad. The pad was washed with ethyl acetate (3 x 100 mL), the combined solutions were evaporated in vacuo and 4- (4-amino-phenyl) -1-piperazinecarboxylic acid, 1,1-dimethylethyl ester Obtained (18 g, 98% yield).
[346] C. 4- (4-isothiocyanatophenyl) -1-piperazinecarboxylic acid, 1,1-dimethylethyl ester
[347] N, N-dimethyl-formamide (120 mL) of 4- (4-aminophenyl) -1-piperazinecarboxylic acid, 1,1-dimethylethyl ester (15 g, 54.1 mmol) (from step B above) The aqueous solution was added dropwise to a solution of cooled (-15 ° C.) 1,1′-thiocarbonyldiimidazole (9.66 g, 54.2 mmol) (Aldrich) in N, N-dimethylformamide (40 mL). After the addition was completed, the cooling bath was removed and the solution was stirred for 1 hour. The mixture was poured into iced water (1 L), stirred for 30 minutes and extracted with diethyl ether. The combined extracts were dried over Na ₂ SO ₄ , evaporated in vacuo to give 4- (4-isothio-cyanatophenyl) -1-piperazinecarboxylic acid, 1,1-dimethylethyl ester (18 g, 94%).
[348] Example 7
[349] 2-bromo-1- [4- (1-piperidinyl) phenyl] ethanone
[350]
[351] A. 2,2-dibromo-1- [4- (1-piperidinyl) phenyl] ethanone
[352] According to the method of Diwu et al. (Tetrahedron Lett. 1998, 39, 4987-4990), 1- [4- (1-piperidinyl) phenyl] ethanone (5 g, 24.6 mmol) (Aldrich) was concentrated to sulfuric acid (25 mL) and the resulting solution was cooled to 0 ° C. Bromine (1.3 mL, 25.4 mmol) was slowly added to this solution at 0 ° C. under stirring. The mixture was slowly warmed up to room temperature and stirred for 6 hours. The reaction mixture was poured into ice water. The precipitate was collected by filtration, washed with water and dried in vacuo to give 2,2-dibromo-1- [4- (1-piperidinyl) phenyl] ethanone (8.2 g, 93%).
[353] B. 2-Bromo-1- [4- (1-piperidinyl) phenyl] ethanone
[354] 2,2-dibromo-1- [4- (1-piperidinyl) phenyl] ethanone (3 g, 8.3 mmol) (from step A above) was dissolved in tetrahydrofuran (15 mL) and 0 Cooled to ° C. To the resulting solution was added triethylamine (1.21 mL, 8.7 mmol) and diethylphosphite (1.13 mL, 8.7 mmol) in tetrahydrofuran (7 mL) dropwise at 0 ° C. under stirring. The mixture was slowly warmed up to room temperature and stirred for 6 hours. The reaction mixture was concentrated in vacuo and poured into ice water. The precipitate was collected by filtration, washed with water and dried in vacuo to afford 2-bromo-1- [4- (1-piperidinyl) phenyl] ethanone (2.3 g, 100% yield).
[355] Example 8
[356] 2-bromo-1- [4- (4-morpholinyl) phenyl] ethanone
[357]
[358] This compound was prepared in the same manner as used to prepare 2-bromo-1- [4- (1-piperidinyl) phenyl] ethanone (Example 7), 1- [4- (4-mor Polyyl) phenyl] ethanone (Aldrich).
[359] Example 9
[360] 2-bromo-1- (3,5-dimethoxyphenyl) ethanone
[361]
[362] A mixture of 1- (3,5-dimethoxyphenyl) ethanone (3.0 g, 16.6 mmol) (Aldrich) and copper (II) bromide (6.2 g, 27.8 mmol) (Aldrich) in ethyl acetate (35 mL) It was heated to reflux under argon for 5 hours. Activated carbon was added to this mixture, and the mixture was stirred and then filtered through Celite ^™ . The filter cake was washed with ethyl acetate, the combined filtrates were evaporated and then purified through silica gel (150 g) in a filter funnel with dichloromethane (500 mL) as eluent, to give 1- (3,5-dimethoxyphenyl Ethanone was obtained (4.9 g, quantitative yield). It was used without further purification.
[363] Example 10
[364] N- [4- (bromoacetyl) phenyl] acetamide
[365]
[366] A solution in bromine (0.31 mL, 5.9 mmol) in carbon tetrachloride (10 mL) was added to a suspension in carbon tetrachloride (20 mL) of N- (4-acetylphenyl) acetamide (1 g, 5.6 mmol) (Lancaster Synthesis). The reaction mixture was stirred for several hours, but only starting material was shown on TLC. Acetic acid (10 mL) was added and the reaction mixture was stirred overnight. Filtration gave N- [4- (bromoacetyl) phenyl] acetamide (1.4 g) as a solid. HPLC showed that its purity was about 80%. This material was used directly in the next step without purification.
[367] Example 11
[368] 1- (1,3-benzodioxol-5-yl) -2-bromoethanone
[369]
[370] In a flask protected from light using an aluminum foil, 8 drops of bromine (1.95 g, 12.2 mmol) in benzene (10 mL) were added 1- (1,3-benzodioxol-5-yl) ethanone ( 2.00 g, 12.2 mmol) (Acros Organics) was added to a solution in benzene (40 mL). The solution was heated to reflux for ˜ 30 seconds, but did not discolor. The remaining bromine solution was added in ˜1 mL aliquots and the solution was left for 2 hours. Ethyl acetate (100 mL) was added and the solution was washed with water and saturated sodium bicarbonate solution (100 mL each) to give a black liquid which solidified upon drying, MgSO ₄ , filtration and evaporation. NMR indicates that this is 1- (1,3-benzodioxol-5-yl) -2,2-dibromoethanone and 1- (1,3-benzodioxol-5-yl) -2-bromoeta Discussion indicated that the mixture was 1: 3. This material was used directly in the next step without purification.
[371] Example 12
[372] 2-bromo-1- (4-hydroxyphenyl) ethanone
[373]
[374] To a solution of 4-hydroxyacetophenone (2.5 g, 18.3 mmol) (Aldrich) in dioxane (10 mL) was added dropwise a solution of bromine (3.22 g, 20.1 mmol) in dioxane (20 mL). The mixture was stirred for 10 minutes, then the mixture was concentrated in vacuo and the residue was recrystallized from methanol to give 2-bromo-1- (4-hydroxyphenyl) ethanone (1.73 g, 44% yield). ).
[375] Example 13
[376] 2-bromo-1- (3-fluoro-4-methoxyphenyl) ethanone
[377]
[378] Solution of bromine (1.13 g, 7.1 mmol) in dioxane (30 mL) in a solution of 3'-fluoro-4'-methoxyacetophenone (1 g, 5.9 mmol) (Aldrich) in dioxane (10 mL) Was added drop wise. After stirring for 10 minutes, the mixture was concentrated in vacuo, and the residue was purified by flash chromatography using a hexane / dichloromethane 10: 4 solution as eluent to afford 2-bromo-1- (3-fluoro-4- Methoxyphenyl) ethanone was obtained as a white powder (910 mg, 63% yield).
[379] Example 14A
[380] 2-bromo-1- (3,5-difluorophenyl) ethanone
[381]
[382] To a solution of 3 ', 5'-difluoroacetophenone (3 g, 19.2 mmol) (Lancaster Synthesis) in dioxane (30 mL), a solution of bromine (3.67 g, 23 mmol) in dioxane (75 mL) Added dropwise. After stirring for 10 minutes, the mixture was concentrated in vacuo, and the residue was purified by flash chromatography using a hexane / dichloromethane 10: 2 solution as eluent to afford 2-bromo-1- (3,5-difluoro Phenyl) ethanone was obtained as a colorless oil (2.9 g, 64% yield).
[383] Example 14B
[384] 1- (4-isothiocyanatophenyl) -4- (2-methoxy-ethyl) piperazine
[385]
[386] A. 1- (1- [2-methoxy-ethyl])-4- (4-nitrophenyl) piperazine
[387] A mixture of 4-chloronitrobenzene (5.5 g, 34.9 mmol) (Aldrich), and 1- (2-methoxy-ethyl) piperazine (5 g, 34.6 mmol) (Chess, GmbH) was heated at 80 ° C. overnight. . Dichloromethane (100 mL) was added and washed with water (3 × 100 mL). The organic layer was dried (Na ₂ SO ₄ ) and concentrated to give a dark red oil. After chromatography (4% methanol in dichloromethane), recrystallization from ethyl acetate / hexanes gave 1- (1- [2-methoxy-ethyl])-4- (4-nitrophenyl) piperazine ( 5.36 g, 58%).
[388] B. 4- [4- (2-methoxy-ethyl) -1-piperazinyl] benzeneamine
[389] 1- (1- [2-methoxy-ethyl])-4- (4-nitrophenyl) piperazine (5.36 g, 20.2 mmol) (from step A above) and 10% palladium / activated carbon, ethanol (125 mL ) Was hydrogenated at 20 psi, room temperature for 1 hour. The catalyst was filtered off and the filter cake was washed thoroughly with ethanol. The mixture was evaporated under reduced pressure to afford 4- [4- (2-methoxy-ethyl) -1-piperazinyl] benzeneamine (4 g, 84%).
[390] C. 1- (4-isothiocyanatophenyl) -4- (1- [2-methoxy-ethyl]) piperazine
[391] N, N-dimethylformamide (60 mL) of 4- [4- (1- [2-methoxy-ethyl])-1-piperazinyl] benzeneamine (4 g, 17 mmol) (from step B above) ) Was added dropwise over 20 minutes to a solution of cooled (-15 ° C.) thiocarbonyldiimidazole (3 g, 17.03 mmol) (Aldrich) in N, N-dimethylformamide (15 mL). After the addition was completed, the mixture was stirred at -15 ° C for 20 minutes, then the cooling bath was removed and the solution was stirred for 1 hour. Ice water (800 ml) was added and the mixture was stirred for 30 minutes. The solid was filtered off, washed with water and dried in vacuo to give 1- (4-isothiocyanatophenyl) -4- (1- [2-methoxy-ethyl]) piperazine (2.8 g, 67%).
[392] Example 14C
[393] 1- (4-isothiocyanatophenyl) -4- (1-methylpropyl) piperazine
[394]
[395] A. 1- (sec-butyl) -4- (4-nitrophenyl) piperazine
[396] 1- (4-nitrophenyl) piperazine (6 g, 28.98 mmol) (Acros Organics), powdered potassium carbonate (2.7 g, 30.4 mmol), 2-bromobutane (3.32 ml, 30.4 mmol) and a catalytic amount A mixture of 18-crown-6 in acetonitrile (45 mL) was heated to reflux overnight. The mixture was filtered and the filter cake was washed with acetonitrile. The solvent was evaporated from the combined filtrates and the residue was partitioned between water and ethyl acetate. Both layers were separated and the aqueous layer was extracted twice with ethyl acetate. The combined organic layers were dried (Na ₂ SO ₄ ), filtered, evaporated and chromatographed (2.5% methanol in dichloromethane) to give 1- (sec-butyl) -4- (4-nitrophenyl) piperazine ( 4.45 g, 58%).
[397] B. 4- [4- (sec-butyl) -1-piperazinyl] benzeneamine
[398] A mixture of 1- (sec-butyl) -4- (4-nitrophenylpiperazine) (4.45 g, 16.9 mmol) (from step A above) and ethanol (125 mL) of 10% palladium / activated carbon was added at 20 psi, room temperature. Hydrogenated at 1.25 h. The catalyst was filtered off and the filter cake was washed thoroughly with ethanol. The mixture was evaporated under reduced pressure to afford 4- [4- (sec-butyl) -1-piperazinyl] benzeneamine (3.55 g, 90%).
[399] C. 1- (4-isothiocyanatophenyl) -4- (sec-butyl) piperazine
[400] Cool the solution of 4- [4- (sec-butyl) -1-piperazinyl] benzeneamine (3.5 g, 15 mmol) (from step B above) in N, N-dimethylformamide (40 mL) 15 ° C.) of thiocarbonyldiimidazole (2.68 g, 15.03 mmol) (Aldrich) was added dropwise over 20 minutes to a solution in N, N-dimethylformamide (15 mL). After the addition was completed, the mixture was stirred at -15 ° C for 20 minutes, then the cooling bath was removed and the solution was stirred for 1 hour. Ice water (800 ml) was added and the mixture was stirred for 30 minutes. The solid was filtered off, washed with water and dried in vacuo to give 1- (4-isothiocyanatophenyl) -4- (sec-butyl) piperazine as a purple solid (3.6 g, 87%).
[401] Example 14D
[402] 4-cyclopentyl-1- (4-isothiocyanatophenyl) piperazine
[403]
[404] A. 1-cyclopentyl-4- (4-nitrophenyl) piperazine
[405] 1- (4-nitrophenyl) piperazine (12 g, 57.9 mmol) (Acros Organics), powdered potassium carbonate (5.4 g, 39 mmol), iodocyclopentane (7 mL, 60.8 mmol) (Aldrich) and The mixture in catalytic amount of 18-crown-6 acetonitrile (90 mL) was heated to reflux overnight. The mixture was filtered and the filter cake was washed with acetonitrile. The solvent was evaporated from the combined filtrates and the residue was partitioned between water and ethyl acetate. Both layers were separated and the aqueous layer was extracted twice with ethyl acetate. The combined organic layers were dried (Na ₂ SO ₄ ), filtered and evaporated to give a yellow solid, and dried under vacuum to give 1-cyclophenyl-4- (4-nitropentyl) piperazine (6.6 g, 41%) .
[406] B. 4- (4-cyclopentyl-1-piperazinyl) benzeneamine
[407] A mixture of 1-cyclopentyl-4- (4-nitrophenyl) piperazine (6.6 g, 23.9 mmol) (from step A above) and ethanol (175 mL) of 10% palladium / activated carbon was 20 psi, 1.25 h at room temperature. Hydrogenated. The catalyst was filtered off and the filter cake was washed thoroughly with ethanol. The mixture was evaporated under reduced pressure to afford 4- (4-cyclopentyl-1-piperazinyl) benzeneamine (5.7 g, 97%).
[408] C. 4-cyclopentyl-1- (4-isothiocyanatophenyl) piperazine
[409] Cool the solution of 4- [4- (1-cyclopentyl) -1-piperazinyl] benzeneamine (5.7 g, 22.4 mmol) (from step B above) in N, N-dimethylformamide (75 mL) -15 ° C.) of thiocarbonyldiimidazole (4 g, 22.5 mmol) (Aldrich) was added dropwise over 20 minutes to a solution in N, N-dimethylformamide (40 mL). After the addition was completed, the mixture was stirred at -15 ° C for 20 minutes, then the cooling bath was removed and the solution was stirred for 1 hour. Pour ice water (1500 ml) and stir for 30 minutes. The precipitate was filtered off, washed with water and dried under vacuum to afford 4-cyclopentyl-1- (4-isothiocyanatophenyl) piperazine (6 g, 93%).
[410] Example 14E
[411] 1- (4-isothiocyanatophenyl) -4- (2-methylpropyl) piperazine
[412]
[413] A. 1- (isobutyl) -4- (4-nitrophenyl) piperazine
[414] 1- (4-nitrophenyl) piperazine (8 g, 38.6 mmol) (Acros Organics), powdered potassium carbonate (3.58g, 25.98 mmol), 1-iodo-2-methylpropane (4.66ml, 40.5 mmol ) And a catalytic amount of a mixture of 18-crown-6 acetonitrile (60 mL) was heated to reflux overnight. The mixture was filtered and the filter cake was washed with acetonitrile. The solvent was evaporated from the combined filtrates and the residue was partitioned between water and ethyl acetate. Both layers were separated and the aqueous layer was extracted twice with ethyl acetate. The combined organic layers were dried (Na ₂ SO ₄ ), filtered, evaporated and dried in vacuo to give 1- (isobutyl) -4- (4-nitrophenyl) piperazine as a yellow solid (9.4 g, 92% ).
[415] B. 4- [4- (isobutyl) -1-piperazinyl] benzeneamine
[416] A mixture of 1- (isobutyl) -4- (4-nitrophenyl) piperazine (9.4 g, 35.7 mmol) (from step A above) and ethanol (175 mL) of 10% palladium / activated carbon at 20 psi, room temperature Hydrogenated 1.25 hours. The catalyst was filtered off and the filter cake was washed thoroughly with ethanol. The mixture was evaporated under reduced pressure to afford 4- [4- (isobutyl) -1-piperazinyl] benzeneamine as a dark red solid (7.8 g, 94%).
[417] C. 1- (4-isothiocyanatophenyl) -4- (1-isobutyl) piperazine
[418] Cool the solution of 4- [4- (isobutyl) -1-piperazinyl] benzeneamine (7.8 g, 33.4 mmol) (from step B above) in N, N-dimethylformamide (75 mL) C) dropwise dropwise addition of thiocarbonyldiimidazole (5.97 g, 33.5 mmol) (Aldrich) to a solution in N, N-dimethylformamide (25 mL) over 20 minutes. After the addition was completed, the mixture was stirred at -15 ° C for 20 minutes, then the cooling bath was removed and the solution was stirred for 1 hour. Ice water (700 ml) was added and the mixture was stirred for 30 minutes. The solid was filtered off, washed with water and dried in vacuo to give 1- (4-isothiocyanatophenyl) -4- (1-isobutyl) piperazine (8 g, 87%).
[419] Example 14F
[420] 4- (3-hydroxypropyl) -1- (4-isothiocyanatophenyl) piperazine
[421]
[422] This compound was prepared using 4- (4-nitrophenyl) -1-piperazine-propanol (Loewe and Mieth Arzneim.-Forsch. 1966, 16, using the hydrogenation reaction and isothiocyanate formation reaction used in Example 4 . Prepared according to the method of 1306-1310). MS (ES) MH ⁺ = 277.
[423] Example 14G
[424] 4-cyclopropylmethyl-1- (4-isothiocyanatophenyl) piperazine
[425]
[426] A. 1- (cyclopropylmethyl) -4- (4-nitrophenyl) piperazine
[427] 1- (4-nitrophenyl) piperazine (10 g, 48.3 mmol) (Acros Organics), powdered potassium carbonate (4.5 g, 32.6 mmol), cyclopropylmethyl bromide (4.93 ml, 50.7 mmol) and a catalytic amount of 18 -A mixture of crown-6 in acetonitrile (75 mL) was heated to reflux overnight. The mixture was filtered and the filter cake was washed with acetonitrile. The solvent was evaporated from the combined filtrates and the residue was partitioned between water and ethyl acetate. Both layers were separated and the aqueous layer was extracted twice with ethyl acetate. The combined organic layers were washed with brine, dried (Na ₂ SO ₄ ), filtered, evaporated and chromatographed (4% methanol in dichloromethane) to give 1- (cyclopropylmethyl) -4- (4-nitrophenyl) pipe Lagine was obtained (4.27 g, 34%).
[428] B. 4- [4- (cyclopropylmethyl) -1-piperazinyl] benzeneamine
[429] A mixture of 1- (cyclopropylmethyl) -4- (4-nitrophenyl) piperazine (4.27 g, 16.3 mmol) (from step A above) and 10% palladium / activated carbon in ethanol (125 mL) was added at 20 psi, room temperature. Hydrogenated at 1 h. The catalyst was filtered off and the filter cake was washed thoroughly with ethanol. The mixture was evaporated under reduced pressure to afford 4- [4- (cyclopropylmethyl) -1-piperazinyl] benzeneamine as a dark red solid (3.7 g, 98%).
[430] C. 1- (4-isothiocyanatophenyl) -4- (1-cyclopropylmethyl) piperazine
[431] Cool the solution of 4- [4- (cyclopropylmethyl) -1-piperazinyl] benzeneamine (3.7 g, 16 mmol) (from step B above) in N, N-dimethylformamide (50 mL) 15 ° C.) of thiocarbonyldiimidazole (2.87 g, 16.1 mmol) (Aldrich) in N, N-dimethylformamide (25 mL) was added dropwise over 20 minutes. After the addition was completed, the mixture was stirred at -15 ° C for 20 minutes, then the cooling bath was removed and the solution was stirred for 1 hour. Ice water (1000 ml) was added and the mixture was stirred for 30 minutes. The solid was filtered off, washed with water and dried in vacuo to give 1- (4-isothiocyanatophenyl) -4- (1-cyclopropylmethyl) piperazine (4 g, 91%).
[432] Example 14H
[433] 2-bromo-1- (3,4,5-trifluoro-phenyl) ethanone
[434]
[435] This compound was prepared from 1- (3,4,5-trifluoro-phenyl) ethanone (ABCR GmbH & Co. KG) according to the method used in Example 13.
[436] Example 14I
[437] 2-bromo-1- (4-fluoro-3-methoxy-phenyl) ethanone
[438]
[439] This compound was prepared from 1- (4-fluoro-3-methoxy-phenyl) ethanone (Apin Chemicals Ltd.) according to the method used in Example 13.
[440] Example 14J
[441] 2-bromo-1- (3-trifluoromethoxy-phenyl) ethanone
[442]
[443] This compound was prepared from 1- (3-trifluoromethoxy-phenyl) ethanone (Aldrich), according to the method used in Example 13.
[444] Example 14K
[445] 2-bromo-1- (3,5-difluoro-4-methoxy-phenyl) ethanone
[446]
[447] This compound was prepared from 1- (3,5-difluoro-4-methoxy-phenyl) ethanone (ABCR GmbH & Co. KG) according to the method used in Example 13.
[448] Example 14L
[449] 2-bromo-1- (3-difluoromethoxy-phenyl) ethanone
[450]
[451] This compound was prepared from 1- (3-difluoromethoxy-phenyl) ethanone (ABCR GmbH & Co. KG) according to the method used in Example 13.
[452] Example 14M
[453] 2-bromo-1- (4-hydroxy-3-fluoro-phenyl) ethanone
[454]
[455] This compound was prepared from 1- (4-hydroxy-3-fluoro-phenyl) ethanone (Apin Chemicals Ltd.) according to the method used in Example 13.
[456] Example 14N
[457] 2-bromo-1- (3-hydroxy-phenyl) ethanone
[458]
[459] This compound was prepared from 1- (3-hydroxy-phenyl) ethanone (Aldrich), according to the method used in Example 13.
[460] Example 14O
[461] 2-bromo-1- (3-ethylphenyl) ethanone
[462]
[463] Under stirring, bromine (383 μl, 7.44 mmol) was added to a solution of 3-ethylacetophenone (Maybridge Chemical Company Ltd .; 1.103 g, 7.44 mmol) in dry 1,4-dioxane (15 mL). The solution was stirred for 30 minutes at room temperature and then the solvent was removed in a rotary evaporator. The residue was chromatographed on a Foxy 200 instrument (Isco, Inc., PO Box 82531, Lincoln, Nebraska 68501, USA .; eluent, 5% CH ₂ Cl ₂ / hexanes, 0-2 minutes, then 20-30% CH ₂ C1 ₂ / hexanes, 2-20 min), 2-bromo-1- (3-ethylphenyl) ethanone were obtained as clear oil (1.15 g, 68%).
[464] Example 15
[465] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (4-chloro-3-nitrophenyl) methanone
[466]
[467] Potassium in a mixture of 1- (4-isothiocyanatophenyl) -4-methylpiperazine (Example 1; 1.0 g, 4.3 mmol) and cyanamide (0.2 g, 4.8 mmol) in acetonitrile (43 mL) A solution of tert-butoxide (43 mL, 0.1 M in tert-BuOH) was added. After 30 minutes at room temperature, 2-bromo-1- (4-chloro-3-nitro-phenyl) ethanone (1.2 g, 4.3 mmol) (Maybridge Chemical Company Ltd.) was added. The reaction mixture was stirred for 4 hours and then poured into 50 mL of water. The product was collected by filtration and washed with water and diethyl ether. The yellow powder was dried in vacuo and [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (4-chloro-3-nitrophenyl) meta 0.9 g of paddy rice was obtained (45% yield).
[468] Example 16
[469] Resin-Bound Thiouronium Salts
[470]
[471] Merrifield resin (77 g, 331 mmol: 4.3 mmol / g Cl loading) (Fluka) according to the method of Obrecht et al. (Helv. Chim. Acta. 1997, 80, 65-72) and A mixture of thiourea (126 g, 1650 mmol) in dioxane / ethanol (4/1) (750 mL) was heated at 85 ° C. for 5 days. The reaction mixture is filtered and the resin is quenched with hot ethanol (2 x 500 mL), ethanol (500 mL), dioxane (2 x 500 mL), hexane (2 x 500 mL) and diethyl ether (2 x 500 mL). It was washed continuously. The pale yellow resin was dried in vacuo to give 115 g of the title resin. The loading of the resin was determined by nitrogen and sulfur analysis: N (7.78), S (9.77).
[472] Example 17
[473] Resin-bound thiourea: the general method
[474]
[475] Resin bound thiouronium salt (Example 16; 1 g, 2.8 mmol, loading capacity: 2.8 mmol / g), isothiocyanate (R ¹ NCS) (5.6 mmol), N, N-diisopropylethylamine (5.6 mmol) (Aldrich), and N, N-dimethylformamide (5.6 mmol, loading capacity: 3.59 mmol / g, manufactured by Argonaut, Inc.), resin-bonded N, N-diisopropyl-ethylamine (12 mL) was shaken gently overnight. The resin was filtered off and washed successively with N, N-dimethylformamide, THF, MeOH, CH ₂ C1 ₂ , and diethyl ether. The resin was dried overnight at 40 ° C./high vacuum to give a resin bound thiourea.
[476] The following resin bound thioureas were prepared from the indicated isothiocyanates according to the general method used in Example 17.
[477]
[478]
[479]
[480] Example 24
[481] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (2,3-dihydro-1,4-benzodioxin-6- Japanese)
[482]
[483] To a solution of cyanamide (44 mg, 1.05 mmol) (Aldrich) in acetonitrile (5 mL) tert-butanol (4 mL) and then 1- (4-isothiocyanatophenyl) -4-methyl piperazine (Example 1; 219 mg, 1 mmol) was added. To this solution potassium t-butoxide (1 M in tert-butanol; 1 mL, 1 mmol) was added and stirred for 30 minutes. 2-bromo-1- (2,3-dihydro-1,4-benzodioxin-6-yl) ethanone (257 mg, 1 mmol) (Maybridge Chemical Company Ltd.) was added and this solution was added. Stir at room temperature for 3 days. The yellow solid was filtered off, washed with cold acetonitrile, then ether, [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (2,3-Dihydro-1,4-benzodioxin-6-yl) methanone was obtained (280 mg, 66%).
[484] Example 25
[485] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (1,3-benzodioxol-5-yl) methanone
[486]
[487] This compound was prepared according to the method used in Example 24: cyanamide (Aldrich), 1- (4-isothiocyanatophenyl) -4-methylpiperazine (Example 1) and 1- (1,3- Prepared from benzodioxol-5-yl) -2-bromoethanone (by Example 11) in 75% yield.
[488] Example 26
[489] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [4- (1-pyrrolidinyl) phenyl] methanone
[490]
[491] To a solution of cyanamide (44 mg, 1.05 mmol) in acetonitrile (5 mL) tert-butanol (4 mL) and then 1- (4-isothiocyanatophenyl) -4-methyl piperazine (Example 1; 219 mg, 1 mmol) was added. To this solution potassium t-butoxide (1 M in 1 tert-butanol; 1 mL, 1 mmol) was added and stirred for 30 minutes. 2-bromo-1- [4- (1-pyrrolidinyl) phenyl] ethanone (257 mg, 1 mmol) (Lancaster) was added and the solution was stirred for 2 days at room temperature. The tan solid was filtered off and washed with cold acetonitrile, water and then cold acetonitrile (solid weight: 271 mg). A portion of the crude product (50 mg) was purified by chromatography (10% methanol / dichloromethane) and [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino ] -5-thiazolyl] [4- (1-pyrrolidinyl) phenyl] methanone was obtained (18.5 mg).
[492] Example 27
[493] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [4- (1-piperidinyl) phenyl] methanone
[494]
[495] Resin-bound thiourea of Example 18 (57 mg, 0.1 mmol, loading capacity: 1.76 mmol / g) and 2-bromo-1- [4- (1-piperidinyl) phenyl] ethanone (Example 7 56 mg, 0.2 mmol) of the mixture in N, N-dimethylformamide (1.5 mL) was shaken overnight. The reaction suspension was filtered and the resin washed with N, N-dimethylformamide and dichloromethane. The solvent was evaporated in vacuo, the resin triturated with diethyl ether / hexane (1/9) and [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] Amino] -5-thiazolyl] [4- (1-piperidinyl) phenyl] methanone was obtained. Mass spectrum (ES) MH ⁺ = 477.
[496] Example 28
[497] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [4- (4-morpholinyl) phenyl] methanone, acetate salt
[498]
[499] This compound was prepared from the resin-bound thiourea and 2-bromo-1- [4- (4-morpholinyl) phenyl] ethanone of Example 18, according to the method used in Example 27 (Example 8). Prepared. The crude product was purified by HPLC using an Amicon C ₁₈ 2 × 5 cm column in the following gradients: A (20 mM NH ₄ OAc / H ₂ O), B (20 mM NH ₄ OAc / CH ₃ CN), A versus Gradient B (10-75% over 10 minutes), flow rate: 20 mL / min. Mass spectrum (ES) MH ⁺ = 479.
[500] Example 29
[501] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (3,4-dihydro-2H-1,5-benzodioxepin- 7-day) Methanone
[502]
[503] This compound was prepared in accordance with the method used in Example 27, and the resin-bound thiourea of Example 18 and 2-bromo-1- (3,4-dihydro-2H-1,5-benzodioxepin-7- Manufactured from ethanone (Maybridge Chemical Company Ltd.). Mass spectrum (ES) MH ⁺ = 466.
[504] Example 30
[505] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl]-(4-hydroxyphenyl) methanone
[506]
[507] This compound was prepared from the resin-bound thiourea and 2-bromo-1- (4-hydroxyphenyl) ethanone (Example 12) of Example 18, according to the method used in Example 27. Mass Spectrum (ES) MH ⁺ = 410.
[508] Example 31
[509] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (3-nitrophenyl) methanone
[510]
[511] This compound was prepared from the resin bound thiourea and 2-bromo-1- (3-nitrophenyl) ethanone (Aldrich) of Example 18, according to the method used in Example 27. Mass spectrum (ES) MH ⁺ = 439.
[512] Example 32
[513] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (4-methoxyphenyl) methanone
[514]
[515] This compound was prepared from the resin-bound thiourea and 2-bromo-1- (4-methoxyphenyl) ethanone (Aldrich) of Example 18, according to the method used in Example 27. Mass spectrum (ES) MH ⁺ = 424.
[516] Example 33
[517] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [4- (diethylamino) phenyl] methanone
[518]
[519] This compound was prepared from the resin bound thiourea and 2-bromo-1- [4- (diethylamino) phenyl] ethanone (Lancaster Synthesis) of Example 18, according to the method used in Example 27. Mass spectrum (ES) MH ⁺ = 465.
[520] Example 34
[521] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (2-dibenzofuranyl) methanone
[522]
[523] This compound was prepared from the resin bound thiourea and 2-bromo-1- (2-dibenzofuranyl) ethanone (Salor) of Example 18, according to the method used in Example 27. Mass spectrum (ES) MH ⁺ = 484.
[524] Example 35
[525] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (3-fluoro-4-methoxyphenyl) methanone
[526]
[527] This compound was prepared from the resin-bound thiourea and 2-bromo-1- (3-fluoro-4-methoxyphenyl) ethanone of Example 18 (Example 13), according to the method used in Example 27. Prepared. Mass spectrum (ES) MH ⁺ = 442.
[528] Example 36
[529] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (3-methoxyphenyl) methanone
[530]
[531] This compound was prepared from the resin-bound thiourea and 2-bromo-1- (3-methoxyphenyl) ethanone (Aldrich) of Example 18, according to the method used in Example 27. Mass spectrum (ES) MH ⁺ = 424.
[532] Example 37
[533] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (3,4-dichlorophenyl) methanone
[534]
[535] This compound was prepared from the resin bound thiourea and 2-bromo-1- (3,4-dichlorophenyl) ethanone (Lancaster Synthesis) of Example 18, according to the method used in Example 27. Mass spectrum (ES) MH ⁺ = 462.
[536] Example 38
[537] [4-amino-2-[[3-fluoro-4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (1,3-benzodioxol-5-yl) Metanon
[538]
[539] This compound was prepared from the resin-bound thiourea of Example 19 and 1- (1,3-benzodioxol-5-yl) -2-bromoethanone (Example 11), according to the method used in Example 27. Prepared. Mass spectrum (ES) MH ⁺ = 456.
[540] Example 39
[541] [4-amino-2-[[3-fluoro-4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (3,5-dimethoxyphenyl) methanone
[542]
[543] This compound was prepared from the resin-bound thiourea and 2-bromo-1- (3,5-dimethoxyphenyl) ethanone of Example 19 (Example 9), according to the method used in Example 27. Mass spectrum (ES) MH ⁺ = 472.
[544] Example 40
[545] [4-amino-2-[[3-fluoro-4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (3-methoxyphenyl) methanone
[546]
[547] This compound was prepared from the resin-bound thiourea and 2-bromo-1- (3-methoxyphenyl) ethanone (Aldrich) of Example 19, according to the method used in Example 27. Mass spectrum (ES) MH ⁺ = 442.
[548] Example 41
[549] [4-amino-2-[[4- [4- (1-methylethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (2,3-dihydro-1,4-benzo Dioxin-6-yl) methanone
[550]
[551] This compound was prepared in accordance with the method used in Example 27, and the resin-bound thiourea and 2-bromo-1- (2,3-dihydro-1,4-benzodioxin-6-yl) of Example 20. Made from ethanone (Maybridge Chemical Company Ltd.). Mass spectrum (ES) MH ⁺ = 480.
[552] Example 42
[553] [4-amino-2-[[4- [4- (1-methylethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (1,3-benzodioxol-5-yl) Metanon
[554]
[555] This compound was prepared from the resin-bound thiourea and 1- (1,3-benzodioxol-5-yl) -2-bromoethanone (Example 11) of Example 20, according to the method used in Example 27. Prepared. Mass spectrum (ES) MH ⁺ = 466.
[556] Example 43
[557] [4-amino-2-[[4- [4- (1-methylethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (3-methoxyphenyl) methanone
[558]
[559] This compound was prepared from the resin-bound thiourea and 2-bromo-1- (3-methoxyphenyl) ethanone (Aldrich) of Example 20, according to the method used in Example 27. Mass spectrum (ES) MH ⁺ = 452.
[560] Example 44
[561] [4-amino-2-[[4- [4- (1-methylethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (3,5-dimethoxyphenyl) methanone
[562]
[563] This compound was prepared from the resin-bound thiourea and 2-bromo-1- (3,5-dimethoxyphenyl) ethanone (Example 9) of Example 20, according to the method used in Example 27. Mass spectrum (ES) MH ⁺ = 482.
[564] Example 45
[565] N- [4-[[4-amino-2-[[4- [4- (1-methylethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl) carbonyl] phenyl] acetamide
[566]
[567] This compound was prepared from the resin-bound thiourea and N- [4- (bromoacetyl) phenyl] acetamide (Example 10) of Example 20, according to the method used in Example 27. Mass spectrum (ES) MH ⁺ = 479.
[568] Example 46
[569] [4-amino-2-[[4- [4- (1-methylethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] [4- (diethylamino) phenyl] methanone
[570]
[571] This compound was prepared from the resin-bound thiourea and 2-bromo-1- [4- (diethylamino) phenyl] ethanone (Lancaster Synthesis) of Example 20, according to the method used in Example 27. Mass spectrum (ES) MH ⁺ = 493.
[572] Example 47
[573] 1-acetyl-4- [4-[[4-amino-5-[(1,3-benzodioxol-5-yl) carbonyl] -2-thiazolyl] amino] phenyl] piperazine
[574]
[575] This compound was prepared from the resin-bound thiourea of Example 21 and 1- (1,3-benzodioxol-5-yl) -2-bromoethanone (Example 11), according to the method used in Example 27. Prepared. Mass spectrum (ES) MH ⁺ = 466.
[576] Example 48
[577] 1-acetyl-4- [4-[[4-amino-5- [4- (diethylamino) benzoyl] -2-thiazolyl] amino] phenyl] piperazine, trifluoroacetate salt
[578]
[579] This compound was prepared from the resin bound thiourea and 2-bromo-1- [4- (diethylamino) phenyl] ethanone (Lancaster Synthesis) of Example 21, according to the method used in Example 27. The crude product was purified by HPLC using an Amicon C ₁₈ 2 × 5 cm column in the following gradients: A (0.05% TFA / H ₂ O), B (0.05% TFA / CH ₃ CN, A to B gradient (10 to 75% over 10 minutes), flow rate: 20 mL / min. Mass spectrum (ES) MH ⁺ = 493.
[580] Example 49
[581] [4-amino-2-[[4- [4- (2-hydroxyethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (2,3-dihydro-1,4- Benzodioxin-6-yl) methanone
[582]
[583] This compound was prepared in accordance with the method used in Example 27, and the resin-bound thiourea and 2-bromo-1- (2,3-dihydro-1,4-benzodioxin-6-yl) of Example 22. Made from ethanone (Maybridge Chemical Company Ltd.). Mass spectrum (ES) MH ⁺ = 482.
[584] Example 50
[585] [4-amino-2-[[4- [4- (2-hydroxyethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] [4- (1-pyrrolidinyl) phenyl] Metanon
[586]
[587] This compound was prepared from the resin-bound thiourea and 2-bromo-1- [4- (1-pyrrolidinyl) phenyl] ethanone (Lancaster Synthesis) of Example 22, according to the method used in Example 27. It was. Mass spectrum (ES) MH ⁺ = 493.
[588] Example 51
[589] [4-amino-2-[[4- [4- (2-hydroxyethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (3-fluorophenyl) methanone
[590]
[591] This compound was prepared from the resin-bound thiourea and 2-bromo-1- (3-fluorophenyl) ethanone of Example 22, according to the method used in Example 27. Mass spectrum (ES) MH ⁺ = 442.
[592] Example 52
[593] [4-amino-2-[[4- [4- (2-hydroxyethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (3,5-difluorophenyl) methanone
[594]
[595] This compound was prepared from the resin-bound thiourea and 2-bromo-1- (3,5-difluorophenyl) ethanone of Example 22 (Example 14), according to the method used in Example 27. . Mass spectrum (ES) MH ⁺ = 460.
[596] Example 53
[597] [4-amino-2-[[4- [4- (2-hydroxyethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (3-methoxyphenyl) methanone
[598]
[599] This compound was prepared from the resin bound thiourea and 2-bromo-1- (3-methoxyphenyl) ethanone (Aldrich) of Example 22, according to the method used in Example 27. Mass spectrum (ES) MH ⁺ = 454.
[600] Example 54
[601] [4-amino-2-[[4- (1-piperazinyl) phenyl] amino] -5-thiazolyl] (3-fluorophenyl) methanone
[602]
[603] A. 4- [4-[[4-amino-5- (3-fluorobenzoyl) -2-thiazolyl] amino] phenyl] -1-piperazine-carboxylic acid, 1,1-dimethylethyl ester
[604] This compound was prepared from the resin bound thiourea and 2-bromo-1- (3-fluorophenyl) ethanone of Maybridge Chemical Company Ltd. of Example 23A, according to the method used in Example 27.
[605] B. [4-amino-2-[[4- (1-piperazinyl) phenyl] amino] -5-thiazolyl] (3-fluorophenyl) methanone
[606] 4- [4-[[4-amino-5- (3-fluorobenzoyl) -2-thiazolyl] amino] phenyl] -1-piperazinecarboxylic acid, 1,1-dimethylethyl ester (75 mg, 0.15 mmol) (from step A) and a mixture of TFA / CH ₂ Cl ₂ (1: 1; 1.5 mL) solution was gently shaken for 1.5 h. The solution was evaporated in vacuo. To the residue was added N, N-diisopropylethylamine (2.5 g, loading capacity: 3.8 mmol / g, manufactured by Argonaut, Inc.) and CH ₂ C1 ₂ (15 mL). After shaking overnight, the mixture was filtered and washed successively with CH ₃ OH and CH ₂ C1 ₂ . The solvent was evaporated in vacuo, the residue was triturated with diethyl ether / hexane (1/9) and [4-amino-2-[[4- (1-piperazinyl) phenyl] amino] -5-thiazolyl ] (3-fluorophenyl) methanone was obtained (60 mg, 100%).
[607] Example 55
[608] [4-amino-2-[[4- (1-piperazinyl) phenyl] amino] -5-thiazolyl] [4- (1-pyrrolidinyl) phenyl] methanone
[609]
[610] The compound was prepared from the resin-bound thiourea and 2-bromo-1- [4- (1-pyrrolidinyl) phenyl] ethanone (LancasterSynthesis) of Example 23A by the method used in Example 54. . Mass spectrum (ES) MH ⁺ = 449.
[611] Example 56
[612] [4-amino-2-[[4- (1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-fluoro-4-methoxyphenyl] methanone
[613]
[614] The compound was prepared from the resin-bound thiourea and 2-bromo-1- (3-fluoro-4-methoxyphenyl) ethanone (Example 13) of Example 23A by the method used in Example 54. Prepared. Mass spectrum (ES) MH ⁺ = 428.
[615] Example 57
[616] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [4- (2-hydroxyethyl) amino-3-nitrophenyl] meta Paddy field
[617]
[618] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] -amino] -5-thiazolyl] [4-chloro-3-nitrophenyl in n-butanol (10 mL) ] Methanone (Example 15; 0.25 g, 0.53 mmol), Ethanolamine (0.13 mL, 2.12 mmol), a mixture of N, N-diisopropylethylamine (Aldrich) (0.37 mL, 2.12 mmol) at 100 ° C Heated overnight. The solvent was evaporated in vacuo and the residue was chromatographed on silica gel using MeOH / CH ₂ Cl ₂ (1/9) as eluent to give 73 mg (28% yield) of the title compound. Mass spectrum (ES) MH ⁺ = 498.
[619] Example 58
[620] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4- (1-pyrrolidinyl) phenyl] methanone
[621]
[622] The compound was prepared from the compound of Example 15 and pyrrolidine by the method used in Example 57. Mass spectrum (ES) MH ⁺ = 508.
[623] Example 59
[624] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4- (4-morpholinyl) phenyl] methanone
[625]
[626] The compound was prepared from the compound of Example 15 and morpholine by the method used in Example 57. Mass spectrum (ES) MH ⁺ = 524.
[627] Example 60
[628] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4-[(2-methoxyethyl) amino] phenyl ] Methanone
[629]
[630] The compound was prepared from the compound of Example 15 and 2-methoxyethanamine (Aldrich) by the method used in Example 57. Mass spectrum (ES) MH ⁺ = 512.
[631] Example 61
[632] Racemic [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4- [3- (hydroxymethyl)- 1-piperidinyl] phenyl] methanone
[633]
[634] The compound was prepared from the compound of Example 15 and racemic 3-piperidinmethanol (Aldrich) by the method used in Example 57. Mass spectrum (ES) MH ⁺ = 552.
[635] Example 62
[636] Racemic [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4- (2-methyl-1-pyrroli Diyl] phenyl] methanone
[637]
[638] The compound was prepared from the compound of Example 15 and racemic 2-methylpyrrolidine (Alfa Aesar) by the method used in Example 57. Mass spectrum (ES) MH ⁺ = 522.
[639] Example 63
[640] (R)-[4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4-[[1- (hydroxy Methyl) -3-methylbutyl] amino] phenyl] methanone
[641]
[642] The compound was prepared from the compound of Example 15 and (R) -2-amino-4-methyl-1-pentanol (Aldrich) by the method used in Example 57. Mass spectrum (ES) MH ⁺ = 554.
[643] Example 64
[644] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4- (4-hydroxy-1-piperidinyl ) Phenyl] methanone
[645]
[646] The compound was prepared from the compound of Example 15 and 4-piperidinol (Aldrich) by the method used in Example 57. Mass spectrum (ES) MH ⁺ = 538.
[647] Example 65
[648] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-amino-4- (4-pyrrolidinyl) phenyl] methanone
[649]
[650] [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4- (1-pyrrolidinyl) phenyl] methanone (Example 58; 0.16 g, 0.32 mmol), a solution of NH ₂ NH ₂ (2 mL), 10% Pd / C (5 mg) and 2-propanol (12 mL) was heated to reflux for 1 hour. The mixture was filtered through a Celite ^™ pad and concentrated in vacuo. Flash chromatography with 4: 1 dichloromethane / methanol [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-amino-4 -(4-pyrrolidinyl) phenyl] methanone (91 mg, 61% yield) was obtained.
[651] Example 66
[652] (R)-[3-amino-4-[[1- (hydroxymethyl) -3-methylbutyl] amino] phenyl] [4-amino-2-[[4- (4-methyl-1-pipera Genyl) phenyl] amino] -5-thiazolyl] methanone
[653]
[654] (R)-[4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4-[[1- (hydroxy Methyl) -3-methylbutyl] amino] phenyl] methanone (Example 63; 30 mg, 0.05 mmol), NH ₂ NH ₂ (0.5 mL), 10% Pd / C (5 mg) and 2-propanol (12 mL) was heated to reflux for 1 h. The mixture was filtered through a Celite ^™ pad and concentrated in vacuo. Flash chromatography with 4: 1 dichloromethane / methanol gave (R)-[3-amino-4-[[1- (hydroxymethyl) -3-methylbutyl] amino] phenyl] [4-amino-2- [ [4- (4-Methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] methanone (15 mg, 53% yield) was obtained.
[655] Example 67
[656] [4-amino-2-[[3-fluoro-4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (2,3-dihydro-1,4-benzo Dioxin-5-yl) methanone
[657]
[658] The compound was prepared by the method used in Example 27, and the resin-bound thiourea of Example 19 and 2-bromo-1- (2,3-dihydro-1,4-benzodioxin-6-yl) Made from ethanone (Maybridge Chemical Company Ltd.). Mass spectrum (ES) MH ⁺ = 470.
[659] Example 68
[660] [4-amino-2-[[3-fluoro-4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [4- (1-pyrrolidinyl) phenyl] meta Paddy field
[661]
[662] The compound was prepared from the resin-bound thiourea and 2-bromo-1- [4- (1-pyrrolidinyl) phenyl] ethanone of Example 19 by the method used in Example 27. . Mass spectrum (ES) MH ⁺ = 481.
[663] Example 69
[664] {4-Amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-methoxy-phenyl) -methanone (phosphate Has)
[665]
[666] {4-Amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-methoxy-phenyl) -methanone (Example 43; 113 mg, 0.25 mmol) was dissolved in hot ethanol (10 ml), to which 25 mg of phosphoric acid in ethanol (1 ml) was added. It was cooled, the crystals collected and dried to {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3 -Methoxy-phenyl) -methanone; A compound with phosphoric acid was obtained. Micro Assays C, H, N, P, and S.
[667] Example 70
[668] {4-Amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-methylsulfanyl-phenyl) -methanone
[669]
[670] 1- (4-isothiocyanatophenyl) -4-methylpiperazine (Example 1; 0.466 g, 2.0 mmol) and cyanamide (0.088 g) in acetonitrile (3 mL) and t-butanol (5 mL) 2.1 mmol) was added to a solution of potassium tert-butoxide (2.0 mL, 1.0 M in tert-BuOH). After 30 minutes at room temperature, 2-bromo-1- (3-methylsulfanylphenyl) ethanone (Rogers, NH et al., Which may be prepared by the method of EP 87953; 0.49 g, 2.0 mmol) was added. The reaction mixture was stirred at rt for 1 h and then refluxed for 15 min. The cold mixture was diluted with ethyl acetate and washed sequentially with saturated aqueous sodium carbonate and brine. The dry (sodium sulfate) solution was evaporated and the residue was chromatographed on silica gel. Elution of the desired product with 9: 1 dichloromethane / methanol and crystallization from ethyl acetate / hexanes gave [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5- 0.439 g (50% yield) of thiazolyl] (3-methylsulfanyl-phenyl) methanone was obtained. Mass spectrum (ES) MH ⁺ = 440.
[671] Example 71
[672] (4-amino-2- {4- [4- (3-hydroxy-propyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3-fluoro-phenyl) -Metanon
[673]
[674] The compound was prepared from the resin-bound thiourea and 2-bromo-1- (3-fluorophenyl) ethanone of Example 23F by Maybridge Chemical Company Ltd. by the method used in Example 27. Mass spectrum (ES) MH ⁺ = 456.
[675] Example 72
[676] (4-amino-2- {4- [4- (3-hydroxy-propyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3-methoxy-phenyl) -Metanon
[677]
[678] The compound was prepared from the resin-bound thiourea and 2-bromo-1- (3-methoxyphenyl) ethanone (Aldrich) of Example 23F by the method used in Example 27. Mass spectrum (ES) MH ⁺ = 468.
[679] Example 73
[680] 3- (4-Amino-2- {4- [4- (3-hydroxy-propyl) -piperazin-1-yl] -phenylamino} -thiazole-5-carbonyl) -benzonitrile
[681]
[682] The compound was prepared by the method used in Example 27, and the resin-bound thiourea and 2-bromo-1- (3-cyanophenyl) ethanone of Example 23F (Aloup, J.-C. et al., WO 9512594 A1, which may be prepared by the method of 19950511). Mass spectrum (ES) MH ⁺ = 463.
[683] Example 74
[684] (4-amino-2- {4- [4- (3-hydroxy-propyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3-nitro-phenyl)- Metanon
[685]
[686] The compound was prepared from the resin-bound thiourea and 2-bromo-1- (3-nitrophenyl) ethanone (commercially available from Aldrich) of Example 23F by the method used in Example 27. Mass spectrum (ES) MH ⁺ = 483.
[687] Example 75
[688] (4-Amino-2- {4- [4- (3-hydroxy-propyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl) -m-tolyl-methanone
[689]
[690] The compound was prepared by the method used in Example 27, by the resin-bound thiourea of Example 23F and 2-bromo-1- (3-methylphenyl) ethanone (Itoh, T. et al., EP 1020426). Can be prepared). Mass spectrum (ES) MH ⁺ = 452.
[691] Example 76
[692] (4-amino-2- {4- [4- (3-hydroxy-propyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3-ethyl-phenyl)- Metanon
[693]
[694] The compound was prepared from the resin-bound thiourea and 2-bromo-1- (3-ethylphenyl) ethanone (Example 14O) of Example 23F by the method used in Example 27. Mass spectrum (ES) MH ⁺ = 466.
[695] Example 77
[696] {4-Amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-ethyl-phenyl) -methanone
[697]
[698] The compound was prepared from the resin-bound thiourea and 2-bromo-1- (3-ethylphenyl) ethanone of Example 18 (Example 14O) by the method used in Example 27. Mass spectrum (ES) MH ⁺ = 422.
[699] Example 78
[700] {4-Amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl} -m-tolyl-methanone
[701]
[702] The compound was prepared by the method used in Example 27, by the resin-bound thiourea of Example 18 and 2-bromo-1- (3-methylphenyl) ethanone (Itoh, T. et al., EP 1020426). Can be prepared). Mass spectrum (ES) MH ⁺ = 408.
[703] Example 79
[704] 3- {4-Amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazole-5-carbonyl} -benzonitrile
[705]
[706] The compound was subjected to the method used in Example 24 to obtain cyanamide, 1- (4-isothio-cyanatophenyl) -4-isopropylpiperazine (Example 3) and 3-cyanophenacyl bromide ( Maybridge Chemical Co. Ltd.). Mass spectrum (ES) MH ⁺ = 447.
[707] Example 80
[708] {4-Amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-fluoro-phenyl) -methanone (hydrogen Bromide)
[709]
[710] The compound was prepared from the resin-bound thiourea and 2-bromo-1- (3-fluoro-phenyl) ethanone (Aldrich) of Example 20 by the method used in Example 27. Mass spectrum (ES) MH ⁺ = 440.
[711] Example 81
[712] {4-Amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3,4,5-trifluoro-phenyl)- Methanone (with hydrogen bromide)
[713]
[714] The compound was prepared by the method used in Example 27, and the resin-bound thiourea of Example 20 and 2-bromo-1- (3,4,5-trifluoro-phenyl) ethanone (Example 14H) Prepared from. Mass spectrum (ES) MH ⁺ = 476.
[715] Example 82
[716] {4-Amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3,5-difluoro-phenyl) -methanone (With hydrogen bromide)
[717]
[718] The compound was prepared from the resin-bound thiourea and 2-bromo-1- (3,5-difluorophenyl) ethanone of Example 20 (Example 14A) by the method used in Example 27. . Mass spectrum (ES) MH ⁺ = 458.
[719] Example 83
[720] 4-amino-2- {4- [4- (2-methoxy-ethyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3-fluoro-4-meth Methoxy-phenyl) -methanone (with hydrogen bromide)
[721]
[722] The compound was prepared by the method used in Example 27, and the resin-bound thiourea and 2-bromo-1- (3-fluoro-4-methoxy-phenyl) ethanone of Example 23B (Example 13) Prepared from. Mass spectrum (ES) MH ⁺ = 486.
[723] Example 84
[724] {4-Amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(4-fluoro-3-methoxy-phenyl)- Methanone (with hydrogen bromide)
[725]
[726] The compound was prepared by the method used in Example 27, and the resin-bound thiourea of Example 20 and 2-bromo-1- (4-fluoro-3-methoxy-phenyl) ethanone (Example 14I) Prepared from. Mass spectrum (ES) MH ⁺ = 470.
[727] Example 85
[728] {4-Amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-fluoro-4-methoxy-phenyl)- Methanone (with hydrogen bromide)
[729]
[730] The compound was prepared by the method used in Example 27, and the resin-bound thiourea of Example 20 and 2-bromo-1- (3-fluoro-4-methoxy-phenyl) ethanone (Example 13) Prepared from. Mass spectrum (ES) MH ⁺ = 470.
[731] Example 86
[732] {4-Amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(4-difluoromethoxy-phenyl) -methanone ( With hydrogen bromide)
[733]
[734] The compound was prepared from the resin-bound thiourea and 2-bromo-1- (4-difluoromethoxy-phenyl) ethanone of Example 20 by the method used in Example 27. Prepared. Mass spectrum (ES) MH ⁺ = 488.
[735] Example 87
[736] {4-Amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-trifluoromethoxy-phenyl) -methanone ( With hydrogen bromide)
[737]
[738] The compound was prepared from the resin-bound thiourea and 2-bromo-1- (3-trifluoromethoxy-phenyl) ethanone of Example 20 (Example 14J) by the method used in Example 27. . Mass spectrum (ES) MH ⁺ = 506.
[739] Example 88
[740] {4-Amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(4-piperidin-1-yl-phenyl)- Methanone (with hydrogen bromide)
[741]
[742] The compound was prepared by the method used in Example 27, and the resin-bound thiourea and 2-bromo-1- (4-piperidin-1-yl-phenyl) ethanone of Example 20 (Example 7) Prepared from. Mass spectrum (ES) MH ⁺ = 505.
[743] Example 89
[744] {4-Amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(4-morpholin-4-yl-phenyl) -meta Paddy field (with hydrogen bromide)
[745]
[746] The compound was prepared from the resin-bound thiourea and 2-bromo-1- (4-morpholin-4-phenyl) ethanone of Example 20 (Example 8) by the method used in Example 27. . Mass spectrum (ES) MH ⁺ = 507.
[747] Example 90
[748] (4-amino-2- {4- [4- (2-methoxy-ethyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3,5-difluoro -Phenyl) -methanone (with hydrogen bromide)
[749]
[750] The compound was prepared from the resin-bound thiourea and 2-bromo-1- (3,5-difluorophenyl) ethanone of Example 23B (Example 14A) by the method used in Example 27. . Mass spectrum (ES) MH ⁺ = 474.
[751] Example 91
[752] (4-amino-2- {4- [4- (2-methoxy-ethyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3,4-difluoro -Phenyl) -methanone (with hydrogen bromide)
[753]
[754] The compound was prepared by the method used in Example 27, and the resin-bound thiourea of Example 23B and 2-bromo-1- (3,4-difluorophenyl) ethanone (Maybridge Chem Co. Ltd.) Prepared from. Mass spectrum (ES) MH ⁺ = 474.
[755] Example 92
[756] (4-amino-2- {4- [4- (2-methoxy-ethyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3-methoxy-phenyl) -Methanone (with hydrogen bromide)
[757]
[758] The compound was prepared from the resin-bound thiourea and 2-bromo-1- (3-methoxy) ethanone (Aldrich) of Example 23B by the method used in Example 27. Mass spectrum (ES) MH ⁺ = 468.
[759] Example 93
[760] (4-amino-2- {4- [4- (2-methoxy-ethyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl) -benzo [1,3] dioxol -5-yl-methanone (with hydrogen bromide)
[761]
[762] The compound was prepared by the method used in Example 27, and the resin-bound thiourea of Example 23B and 2-bromo-1- (benzo [1,3] dioxol-5-yl) ethanone (Example 11 From). Mass spectrum (ES) MH ⁺ = 482.
[763] Example 94
[764] (4-amino-2- {4- [4- (2-methoxy-ethyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(2,3-dihydro- Benzo [1,4] dioxin-6-yl) -methanone (with hydrogen bromide)
[765]
[766] The compound was prepared by the method used in Example 27, and 2-bromo-1- (2,3-dihydro-benzo [1,4] dioxine-6-yl with the resin-bound thiourea of Example 23B. It was prepared from ethanone (Maybridge Chem Co. Ltd.). Mass spectrum (ES) MH ⁺ = 496.
[767] Example 95
[768] {4-Amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3,5-difluoro-phenyl) -methanone ( With hydrogen bromide)
[769]
[770] The compound was prepared from the resin-bound thiourea of Example 18 and 2-bromo-1- (3,5-difluorophenyl) ethanone (Example 14A) by the method used in Example 27. . Mass spectrum (ES) MH ⁺ = 430.
[771] Example 96
[772] {4-Amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3,5-difluoro-4-methoxy-phenyl ) -Methanone (with hydrogen bromide)
[773]
[774] The compound was prepared by the method used in Example 27, and the resin-bound thiourea of Example 18 and 2-bromo-1- (3,5-difluoro-4-methoxy-phenyl) ethanone Example 14K). Mass spectrum (ES) MH ⁺ = 460.
[775] Example 97
[776] {4-Amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(4-fluoro-3-methoxy-phenyl) -meta Paddy field (with hydrogen bromide)
[777]
[778] The compound was prepared by the method used in Example 27, and the resin-bound thiourea of Example 18 and 2-bromo-1- (4-fluoro-3-methoxy-phenyl) ethanone (Example 14I) Prepared from. Mass spectrum (ES) MH ⁺ = 442.
[779] Example 98
[780] {4-Amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-trifluoromethoxy-phenyl) -methanone (hydro Gen bromide)
[781]
[782] The compound was prepared from the resin-bound thiourea of Example 18 and 2-bromo-1- (3-trifluoromethoxy-phenyl) ethanone (Example 14J) by the method used in Example 27. . Mass spectrum (ES) MH ⁺ = 478.
[783] Example 99
[784] (4-amino-2- {4- [4- (2-hydroxy-ethyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3-fluoro-phenyl) -Methanone (with acetic acid)
[785]
[786] The compound was prepared from the resin bound thiourea and 2-bromo-1- (3-fluoro-phenyl) ethanone (Aldrich) of Example 22 by the method used in Example 27. The crude product was purified by HPLC as described in Example 28. Mass spectrum (ES) MH ⁺ = 442.
[787] Example 100
[788] [4-amino-2- (4-piperazin-1-yl-phenylamino) -thiazol-5-yl]-(3,4,5-trifluorophenyl) -methanone (with acetic acid)
[789]
[790] The compound was prepared by the method used in Example 54, and the resin-bound thiourea of Example 23A and 2-bromo-1- (3,4,5-trifluoro-phenyl) ethanone (Example 14H) Prepared from. The crude product was purified by HPLC as described in Example 28. Mass spectrum (ES) MH ⁺ = 434.
[791] Example 101
[792] [4-Amino-2- (4-piperazin-1-yl-phenylamino) -thiazol-5-yl]-(3,5-difluoro-4-methoxy-phenyl) -methanone (acetic acid Has
[793]
[794] The compound was prepared by the method used in Example 54, and the resin-bound thiourea of Example 23A and 2-bromo-1- (3,5-difluoro-4-methoxy-phenyl) ethanone Example 14K). The crude product was purified by HPLC as described in Example 28. Mass spectrum (ES) MH ⁺ = 446.
[795] Example 102
[796] [4-amino-2- (4-piperazin-1-yl-phenylamino) -thiazol-5-yl]-(4-fluoro-3-methoxy-phenyl) -methanone (with acetic acid)
[797]
[798] The compound was prepared by the method used in Example 54, and the resin-bound thiourea and 2-bromo-1- (4-fluoro-3-methoxy-phenyl) ethanone of Example 23A (Example 14I). Prepared from. The crude product was purified by HPLC as described in Example 28. Mass spectrum (ES) MH ⁺ = 428.
[799] Example 103
[800] [4-amino-2- (4-piperazin-1-yl-phenylamino) -thiazol-5-yl]-(3-trifluoromethoxy-phenyl) -methanone (with acetic acid)
[801]
[802] The compound was prepared from the resin-bound thiourea and 2-bromo-1- (3-trifluoromethoxy-phenyl) ethanone (Example 14J) of Example 23A by the method used in Example 54. . The crude product was purified by HPLC as described in Example 28. Mass spectrum (ES) MH ⁺ = 464.
[803] Example 104
[804] {4-Amino-2- [4- (4-sec-butyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-fluoro-phenyl) -methanone
[805]
[806] The compound was prepared from the resin-bound thiourea and 2-bromo-1- (3-fluoro-phenyl) ethanone (Aldrich) of Example 23C by the method used in Example 27. Mass spectrum (ES) MH ⁺ = 454.
[807] Example 105
[808] {4-Amino-2- [4- (4-sec-butyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl} -benzo [1,3] dioxol-5-yl- Metanon
[809]
[810] The compound was prepared by the method used in Example 27, and the resin-bound thiourea of Example 23C and 2-bromo-1- (benzo [1,3] dioxol-5-yl) ethanone (Example 11 From). Mass spectrum (ES) MH ⁺ = 480.
[811] Example 106
[812] {4-Amino-2- [4- (4-sec-butyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(2,3-dihydro-benzo [1,4 Dioxin-6-yl) -Methanone
[813]
[814] The compound was prepared by the method used in Example 27, and 2-bromo-1- (2,3-dihydro-benzo [1,4] dioxine-6-yl with the resin-bound thiourea of Example 23C. Ethanone from Maybridge Chem Co. Ltd. Mass spectrum (ES) MH ⁺ = 494.
[815] Example 107
[816] {4-Amino-2- [4- (4-cyclopentyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl} -benzo [1,3] dioxol-5-yl-meta Paddy field
[817]
[818] The compound was prepared by the method used in Example 27, and the resin-bound thiourea of Example 23D and 2-bromo-1- (benzo [1,3] dioxol-5-yl) ethanone (Example 11 From). Mass spectrum (ES) MH ⁺ = 492.
[819] Example 108
[820] {4-Amino-2- [4- (4-cyclopentyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(2,3-dihydro-benzo [1,4] Dioxin-6-yl) -Methanone
[821]
[822] The compound was prepared by the method used in Example 27, and 2-bromo-1- (2,3-dihydro-benzo [1,4] dioxine-6-yl with the resin-bound thiourea of Example 23D. It was prepared from ethanone (Maybridge Chem Co. Ltd.). Mass spectrum (ES) MH ⁺ = 506.
[823] Example 109
[824] {4-Amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-trifluoromethoxy-phenyl) -methanone
[825]
[826] The compound was prepared from the resin-bound thiourea and 2-bromo-1- (3-trifluoromethoxy-phenyl) ethanone of Example 20 (Example 14J) by the method used in Example 27. . Mass spectrum (ES) MH ⁺ = 506.
[827] Example 110
[828] {4-Amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-fluoro-phenyl) -methanone
[829]
[830] The compound was prepared from the resin-bound thiourea and 2-bromo-1- (3-fluoro-phenyl) ethanone (Aldrich) of Example 20 by the method used in Example 27. Mass spectrum (ES) MH ⁺ = 440.
[831] Example 111
[832] {4-Amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-difluoromethoxy-phenyl) -methanone
[833]
[834] The compound was prepared from the resin-bound thiourea of Example 20 and 2-bromo-1- (3-difluoromethoxy-phenyl) ethanone (Example 14L) by the method used in Example 27. . Mass spectrum (ES) MH ⁺ = 488.
[835] Example 112
[836] {4-Amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-hydroxy-phenyl) -methanone (acetic acid Has)
[837]
[838] The compound was prepared from the resin-bound thiourea and 2-bromo-1- (3-hydroxy-phenyl) ethanone of Example 20 (Example 14N) by the method used in Example 27. The crude product was purified by HPLC as described in Example 28. Mass spectrum (ES) MH ⁺ = 438.
[839] Example 113
[840] {4-Amino-2- [4- (4-isobutyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-hydroxy-phenyl) -methanone (acetic acid Has)
[841]
[842] The compound was prepared from the resin bound thiourea and 2-bromo-1- (3-hydroxy-phenyl) ethanone (Example 14N) of Example 23E by the method used in Example 27. The crude product was purified by HPLC as described in Example 28. Mass spectrum (ES) MH ⁺ = 452.
[843] Example 114
[844] [4-amino-2- (4-piperazin-1-yl-phenylamino) -thiazol-5-yl]-(3-difluoromethoxy-phenyl) -methanone
[845]
[846] The compound was prepared from the resin-bound thiourea and 2-bromo-1- (3-difluoromethoxy-phenyl) ethanone (Example 14L) of Example 23A by the method used in Example 54. . Mass spectrum (ES) MH ⁺ = 446.
[847] Example 115
[848] {4-Amino-2- [4- (4-cyclopropylmethyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-difluoromethoxy-phenyl) -methanone
[849]
[850] The compound was subjected to the method used in Example 24 to obtain cyanamide, 1- (4-isothiocyanato-phenyl) -4-cyclopropylmethylpiperazine (Example 14G) and 2-bromo-1 Prepared from-(3-difluoromethoxyphenyl) ethanone (Example 14L). Mass spectrum (ES) MH ⁺ = 500.
[851] Example 116
[852] {4-Amino-2- [4- (4-cyclopropylmethyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-methoxy-phenyl) -methanone
[853]
[854] The compound was subjected to the method used in Example 24 to obtain cyanamide, 1- (4-isothiocyanato-phenyl) -4-cyclopropylmethylpiperazine (Example 14G) and 2-bromo-1 Prepared from-(3-methoxyphenyl) ethanone (Aldrich). Mass spectrum (ES) MH ⁺ = 464.
[855] Example 117
[856] {4-Amino-2- [4- (4-cyclopropylmethyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl} -benzo [1,3] dioxol-5-yl- Metanon
[857]
[858] The compound was subjected to the method used in Example 24 to obtain cyanamide, 1- (4-isothiocyanato-phenyl) -4-cyclopropylmethylpiperazine (Example 14G) and 2-bromo-1 Prepared from-(benzo [1,3] dioxol-5-yl) ethanone (Example 11). Mass spectrum (ES) MH ⁺ = 478.
[859] Example 118
[860] {4-Amino-2- [4- (4-cyclopropylmethyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(2,3-dihydro-benzo [1,4 Dioxin-6-yl) -Methanone
[861]
[862] The compound was subjected to the method used in Example 24 to obtain cyanamide, 1- (4-isothiocyanato-phenyl) -4-cyclopropylmethylpiperazine (Example 14G) and 2-bromo-1 Prepared from-(2,3-dihydro-benzo [1,4] dioxin-6-yl) ethanone (Maybridge Chemical Company Ltd.). Mass spectrum (ES) MH ⁺ = 492.
[863] Example 119
[864] {4-Amino-2- [4- (4-cyclopropylmethyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-fluoro-4-hydroxy-phenyl) -Metanon
[865]
[866] The compound was subjected to the method used in Example 24 to obtain cyanamide, 1- (4-isothiocyanato-phenyl) -4-cyclopropylmethylpiperazine (Example 14G) and 2-bromo-1 Prepared from-(3-fluoro-4-hydroxyphenyl) ethanone (Example 14M). Mass spectrum (ES) MH ⁺ = 468.
[867] Example 120
[868] {4-Amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-difluoromethoxy-phenyl) -methanone
[869]
[870] The compound was subjected to the method used in Example 24 to obtain cyanamide, 1- (4-isothiocyanato-phenyl) -4-methylpiperazine (Example 1) and 2-bromo-1- ( Prepared from 3-difluoro-methoxy-phenyl) ethanone (Example 14L). Mass spectrum (ES) MH ⁺ = 460.
[871] Example 121
[872] {4-Amino-2- [4- (4-cyclopropylmethyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-fluoro-phenyl) -methanone
[873]
[874] The compound was subjected to the method used in Example 24 to obtain cyanamide, 1- (4-isothiocyanato-phenyl) -4-cyclopropylmethylpiperazine (Example 14G) and 2-bromo-1 Prepared from-(3-fluoro-phenyl) ethanone (Aldrich). Mass spectrum (ES) MH ⁺ = 452.
[875] Example 122
[876] {4-Amino-2- [4- (4-cyclopropylmethyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-fluoro-4-methoxy-phenyl) -Metanon
[877]
[878] The compound was subjected to the method used in Example 24 to obtain cyanamide, 1- (4-isothiocyanato-phenyl) -4-cyclopropylmethylpiperazine (Example 14G) and 2-bromo-1 Prepared from-(3-fluoro-4-methoxyphenyl) ethanone (Example 13). Mass spectrum (ES) MH ⁺ = 482.
[879] Example 123
[880] {4-Amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-hydroxy-phenyl) -methanone
[881]
[882] The compound was subjected to the method used in Example 24 to obtain cyanamide, 1- (4-isothiocyanato-phenyl) -4-methylpiperazine (Example 1) and 2-bromo-1- ( Prepared from 3-hydroxyphenyl) ethanone (Example 14N). Mass spectrum (ES) MH ⁺ = 410.
[883] Example 124
[884] 3- {4-Amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazole-5-carbonyl} -benzonitrile
[885]
[886] The compound was subjected to the method used in Example 24 to obtain cyanamide, 1- (4-isothiocyanato-phenyl) -4-methylpiperazine (Example 1) and 3-cyanophenacyl bromide (Maybridge Chemical Co. Ltd.). Mass spectrum (ES) MH ⁺ = 419.
[887] Example 125
[888] Antiproliferative activity
[889] The antiproliferative activity of the compounds of the invention is demonstrated below. These activities indicate that the compounds of the present invention are useful for the treatment of cancer, especially solid tumors such as breast and colon tumors.
[890] Kinase Assay
[891] To measure inhibition of Cdk4, Cdk2 and Cdk1 activity, kinase assays were performed using the FlashPlate ^™ assay (NEN ^™ -Life Science). FlashPlate assays were performed using recombinant human cyclin B-CDK1, human cyclin E-CDK2 or human cyclin D1-CDK4 complexes. GST-cycline E (GST-cycE), CDK2, GST-cyclin B (GST-cycB), CDK1, GST-CDK4 and cyclin D1 (cycD1) cDNA clones in baculovirus vectors were identified by Baylor College of Medicine ( From Dr. W. Harper in Houston, TX). Proteins are co-expressed in High Five ^™ insect cells, and complexes on glutathione Sepharose resin (Piscataway, Pharmacia, NJ) as previously described (Harper, JW et al., Cell 1993, 75, 805-816). Purified. The 6x-histidine labeled cleaved form (amino acids 386-928) of the retinoblastoma (Rb) protein was used as substrate for the cycD1-CDK4, cycB-CDK1 and cycE-CDK2 assays (expression plasmids, Welwyn Garden City, United Kingdom Provided by Dr. Veronica Sullivan at the Roche Research Centre, Department of Molecular Virology). Rb protein is a natural substrate for phosphorylation by CDK4, CDK2 and CDK1 (see Herwig and Strauss Eur. J. Biochem. Vol . 246 (1997) pp . 581-601 and references cited therein).
[892] Expression of the 62 Kd protein was under the control of the IPTG inducible promoter in the M15 Escherichia coli strain. Purification was performed by lysing the cells and binding the lysates at pH 8.0 to a Ni chelated agarose column pretreated with 1 mM imidazole. The resin was then washed several times with pH buffer, gradually decreasing the pH buffer to pH 6.0 and eluted with 500 mM imidazole. Eluted protein was dialyzed against 20 mM HEPES pH 7.5, 30% glycerol, 200 mM NaCl, and 1 mM DTT. Purified Rb fusion protein stocks were quantified for protein concentration, aliquoted and stored at -70 ° C.
[893] For all three kinase assays reported herein, 96 well FlashPlate was applied with 10 μg / ml Rb protein, 100 μl per well. The plates were incubated on shaker at 4 ° C. overnight or at room temperature for 3 hours. For control of nonspecific phosphorylation, one row of wells was applied with 100 μl / well coating buffer (20 mM HEPES, 0.2 M NaCl). The plates were then washed twice with wash buffer (0.01% Tween 20 in phosphate buffered saline). The compound to be tested (“test compound”) was added to the wells at 5 × final concentration. Sufficient amount of enzyme and 40 μl reaction mixture (25 mM HEPES, 20 mM MgCl ₂ , 0.002% Tween 20, 2 mM DTT, 1 μM ATP, 4 nM ³³ ) to obtain a count 10 times greater than the background The reaction was initiated by the immediate addition of P-ATP). The plate was incubated for 30 minutes at room temperature on a shaker. Plates were washed four times with wash buffer, sealed and counted on TopCount scintillation counter (Packard Instrument CO., Downers Grove, IL). Percent inhibition of Rb phosphorylation, a measure of inhibition of CDK activity, was determined according to the following formula:
[894]
[895] (Wherein, "test compound" refers to the average number per minute of the test replica, "non-specific" refers to the average number per minute when no cyclin D / Cdk4 or the like is added and "total" means no compound at all) The average number per minute). The IC ₅₀ value is the concentration of test compound that reduces the protein-kinase induced incorporation of radioisotope identification by 50% under the test conditions described.
[896] The results from the in vitro experiment described above are shown in Table 1 below.
[897] IC ₅₀ values are summarized in Table 1 below.
[898] Cell Based Assay (Tetrazolium Dye Proliferation Assay)
[899] Proliferation was assessed by a tetrazolium dye assay following the procedures of Denizot and Lang (Denizot, F. and Lang, R. J Immunol Methods 1986, 89, 271-277). The cell line used was HCT116, a colorectal carcinoma cell line obtained from the American Type Cell Culture Collection (ATCC; Rockville, MD). The cells were grown in McCoy's 5A medium supplemented with 10% FCS and L-glutamine.
[900] Cells were plated at appropriate inoculation concentrations and grown exponentially over the course of the assay in 96 well tissue culture plates. The plate was incubated overnight at 37 ° C. in a humidified incubator with 5% CO ₂ . The next day, test compounds were serially diluted to 4 times the final concentration in a suitable medium containing 1.2% DMSO. One quarter of the final volume of each dilution was added to the plate containing the cells. The same dose of 1.2% DMSO in the medium was added to one row of "control group wells" so that the final concentration of DMSO in each well was 0.3%. Wells in which no cells were added were provided as "blanks. Wells in which no inhibitors were added were provided as" inhibitor control groups. "The plates were returned to the incubator and (determined by their growth curves). At the set time point the plates were analyzed as described below.
[901] 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl-2H-tetrazolium bromide (thiazolyl blue; MTT; Sigma) was added to each well to give a final concentration of 1 mg / It made into ml. The plate was returned to the incubator at 37 ° C. for 2.5 to 3 hours. The MTT containing medium was removed and the resulting forazon metabolite was solubilized with 100% ethanol with shaking for 15 minutes at room temperature. Absorbance recording was taken at a wavelength of 570 nm in a microtiter plate recorder (Dynatech and Molecular Devices plate recorders were used interchangeably) with 650 nm as the reference wavelength. Percent inhibition (% INH) is calculated as the ratio of the average absorbance (S _AVE ) of each test replicate to the mean value of the control (C _AVE ) after subtracting the absorbance of the blank wells in all wells from 1.00 to 뺌. The final number was then multiplied by 100 (% INH = (1.00 − S _AVE / C _AVE ) × 100). The concentration at which 50% inhibition of cell proliferation is obtained (IC ₅₀ ) is determined from linear regression of the plot of logarithm of concentration versus percent inhibition. IC ₅₀ values are also shown in Table 1 below.
[902] Table 1 IC ₅₀ and on the cell basic analysis of the embodiment of compound in CDK4, CDK2, and CDK1 kinase black ( "MTT") also shows IC ₅₀ in black.
[903]
[904]
[905]
[906] Example 126
[907] Tablet formulation
[908] Itemingredientmg / tablet OneCompound A *525100250500750 2Anhydrous lactose1038335193857 3Croscarmellose sodium668163248 4Povidone K30556122436 5Magnesium stearateOneOneOne369Total weight120120150300600900 Compound A represents a compound of the present invention
[909] Manufacturing procedure:
[910] 1. Mix items 1, 2, and 3 in a suitable mixer for 15 minutes.
[911] 2. Granulate the powder mixture in step 1 with 20% povidone K30 solution (item 4).
[912] 3. Dry the granulate in step 2 at 50 ° C.
[913] 4. Pass the granulation in step 3 through a suitable milling equipment.
[914] 5. Add item 5 to the 4 milled granulated granules and mix for 3 minutes.
[915] 6. Press the granulate in step 5 with a suitable press.
[916] Example 127
[917] Capsule Formulation
[918] Itemingredientmg / capsules OneCompound A *525100250500 2Anhydrous lactose159123148-- 3Corn starch2535403570 4Talc1015101224 5Magnesium stearateOne2236Total filling weight200200300300600 * Compound A represents a compound of the present invention.
[919] Manufacturing procedure:
[920] 1. Mix items 1, 2, and 3 in a suitable mixer for 15 minutes.
[921] 2. Add items 4 and 5 and mix for 3 minutes.
[922] 3. Fill into a suitable capsule.
[923] Example 128
[924] Injection / Emulsion Formulations
[925] Itemingredientmg / ml OneCompound A *1 mg 2PEG 40010 to 50 mg 3lecithin20-50 mg 4Soybean oil1 to 5 mg 5Glycerol8-12 mg 6Water1 ml * Compound A represents a compound of the present invention.
[926] Manufacturing procedure:
[927] 1. Dissolve item 1 in item 2.
[928] 2. Add items 3, 4, and 5 to item 6, mix until dispersed, and homogenize.
[929] 3. Add the solution from step 1 to the mixture from step 2 and homogenize until the dispersion is translucent.
[930] 4. Sterile filtered through a 0.2 μm filter and filled into vials.
[931] Example 129
[932] Injection / Emulsion Formulations
[933] Itemingredientmg / ml OneCompound A *1 mg 2Glycofurol10 to 50 mg 3lecithin20-50 mg 4Soybean oil1 to 5 mg 5Glycerol8-12 mg 6waterCorrect amount, 1 ml * Compound A represents a compound of the present invention.
[934] Manufacturing procedure:
[935] 1. Dissolve item 1 in item 2.
[936] 2. Add items 3, 4, and 5 to item 6, mix until dispersed, and homogenize.
[937] 3. Add the solution from step 1 to the mixture from step 2 and homogenize until the dispersion is translucent.
[938] 4. Sterile filtered through a 0.2 μm filter and filled into vials.
[939] While the invention has been described with reference to specific and preferred embodiments, those skilled in the art will recognize that variations and modifications may be made to the customary experiments and practices of the invention. Accordingly, the invention is not intended to be limited by the foregoing description, but is intended to be defined by the appended claims and their equivalents.

权利要求:
Claims (35)
[1" claim-type="Currently amended] A compound of formula (I), or a pharmaceutically acceptable salt or ester thereof:
[Formula I]
Wherein R ¹ is selected from the group consisting of lower alkyl, H, cycloalkyl, COR ⁹ , and SO ₂ R ¹⁰ which may be optionally substituted with a group selected from OR ⁶ , cycloalkyl, and NR ⁷ R ⁸ ;
R ² is selected from the group consisting of H, F, Cl, and CH ₃ ;
R ³ , R ⁴ and R ⁵ are each independently lower alkyl, H, OR ¹¹ , NR ¹² R ¹³ , halogen, NO ₂ , CONR ⁶ R which may be optionally substituted with a group selected from OR ⁶ and NR ⁷ R ^{8 9} , NHSO ₂ R ¹⁴ , CN, S-lower alkyl, OCF ₃ , and OCHF ₂ ,
Or alternatively, R ³ and R ⁴ taken together with a bond and two carbons between them from the benzene ring (D) to which R ³ and R ⁴ are attached are a ring system having up to two additional rings It can form, and wherein said rings each having a 5 to 7 ring atoms, and the ring attached to the benzene ring (D) includes any one or more heteroatoms, and optionally substituted by lower alkyl, with the proviso that, R ³ and R ⁴ are not simultaneously -OCH _3, also R ³ is -NO ₂ days when R ⁴ is not a -Cl;
R ⁶ and R ⁹ are independently selected from the group consisting of lower alkyl, optionally substituted with OH and halogen, and H;
R ⁷ and R ⁸ are independently selected from the group consisting of lower alkyl which may be optionally substituted with OR ⁶ , and, alternatively, R ⁷ is H and R ⁸ is OH, or alternatively , NR ⁷ R ⁸ may optionally form a ring having 5 to 6 atoms, the ring optionally containing one or more additional heteroatoms and one or more OR ⁶ and lower itself optionally substituted by OH Optionally substituted by a group consisting of alkyl;
R ¹⁰ is selected from the group consisting of lower alkyl, which may be optionally substituted by one or more chlorine or fluorine, and NH ₂ ;
R ¹¹ is selected from the group consisting of OR ⁶ , COOH, halogen and lower alkyl optionally substituted with a group selected from NR ¹⁵ R ¹⁶ , and H;
R ¹² and R ¹³ are independently selected from the group consisting of lower alkyl, H, COR ¹⁷ , and SO ₂ R ¹⁸ which may be optionally substituted with a group selected from OR ⁶ , COOH and NR ¹⁵ R ¹⁶ , provided that R is Only one of ¹² and R ¹³ is COR ¹⁷ or SO ₂ R ¹⁸ , or alternatively NR ¹² R ¹³ may optionally form a ring having 5 to 6 atoms, the ring having one or more additional heteroatoms Optionally substituted by a group consisting of one or more OR ⁶ and lower alkyl, which may itself be optionally substituted with OH;
R ¹⁴ is lower alkyl;
R ¹⁵ and R ¹⁶ are independently selected from the group consisting of lower alkyl which may be optionally substituted with OH, and H, or alternatively NR ¹⁵ R ¹⁶ may optionally form a ring having 5 to 6 atoms Wherein the ring is optionally substituted by a group consisting of one or more additional hetero atoms and optionally one or more OR ⁶ and lower alkyl, which may itself be optionally substituted with OH;
R ¹⁷ is selected from the group consisting of lower alkyl, which may be optionally substituted with groups selected from OH, COOH, and NR ¹⁵ R ¹⁶ , and H;
R ¹⁸ is lower alkyl).
[2" claim-type="Currently amended] The compound of claim 1, wherein R ¹ is H, methyl, CH ₂ CH ₂ OH, CH ₂ CH ₂ CH ₂ OH, CH ₃ CO—, CH (CH ₃ ) ₂ , CH ₂ CH (CH ₃ ) ₂ , cyclopropyl Compound selected from the group consisting of methyl and CH ₃ .
[3" claim-type="Currently amended] A compound according to claim 1 or 2, wherein R ² is selected from the group consisting of H and fluorine.
[4" claim-type="Currently amended] The compound according to claim 1, wherein R ³ is selected from the group consisting of OR ¹¹ , lower alkyl, NH ₂ , Cl, F, H, OCHF ₂ and NO ₂ .
[5" claim-type="Currently amended] The compound according to any one of claims 1 to ⁴ , wherein R ⁴ is acetamido, chloro, diethylamino, hydrogen, hydroxy, hydroxyethylamino, [1- (hydroxymethyl) -3-methylbutyl ] Amino, 1- (3-hydroxymethyl) piperidinyl, 4-hydroxy-1-piperidinyl, methoxy, 2-methoxyethylamino, 2-methyl-1-pyrrolidinyl, morpholino , Piperidinyl, pyrrolidinyl compound, characterized in that selected from the group consisting of.
[6" claim-type="Currently amended] The compound according to any one of claims 1 to 5, wherein R ³ and R ⁴ taken together with the benzene ring to which they are attached form a polycyclic ring system.
[7" claim-type="Currently amended] The ring system of claim 1, wherein the ring system is 2-dibenzofuranyl, 1,3-benzodioxol-5-yl, 2,3-dihydro-1,4-benzodioxin- 6-yl, and 3,4-dihydro-2H-1,5-benzodioxepin-7-yl.
[8" claim-type="Currently amended] 8. A compound according to any one of claims 1 to 7, which is selected from the group consisting of:
a) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (2,3-dihydro-1,4-benzodioxin- 6-day) metanon,
b) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (1,3-benzodioxol-5-yl) methanone,
c) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (3,4-dihydro-2H-1,5-benzodiox Sepin-7-yl) methanone,
d) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (2-dibenzofuranyl) methanone,
e) [4-amino-2-[[3-fluoro-4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (1,3-benzodioxol-5- Methanon,
f) [4-amino-2-[[4- [4- (1-methylethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (2,3-dihydro-1,4 -Benzodioxine-6-yl) methanone,
g) [4-amino-2-[[4- [4- (1-methylethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (1,3-benzodioxol-5- Methanon,
h) 1-acetyl-4- [4-[[4-amino-5-[(1,3-benzodioxol-5-yl) carbonyl] -2-thiazolyl] amino] phenyl] piperazine,
i) [4-amino-2-[[4- [4- (2-hydroxyethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (2,3-dihydro-1, 4-benzodioxin-6-yl) methanone,
j) [4-amino-2-[[3-fluoro-4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (2,3-dihydro-1,4 -Benzodioxine-5-yl) methanone,
k) (4-amino-2- {4- [4- (2-methoxy-ethyl) -piperazin-1-yl] phenylamino} -thiazol-5-yl) -benzo [1,3] di Oxol-5-yl-methanone,
l) 4-amino-2- {4- [4- (2-methoxy-ethyl) -piperazin-1-yl] phenylamino} -thiazol-5-yl) (2,3-dihydro-benzo [1,4] dioxin-6-yl) -methanone,
m) {4-amino-2- [4- (4-sec-butyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl} -benzo [1,3] dioxol-5- Methanol,
n) {4-amino-2- [4- (4-sec-butyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(2,3-dihydro-benzo [1 , 4] dioxin-6-yl) -methanone,
o) {4-amino-2- [4- (4-cyclopentyl-piperazin-1-yl) -phenylamino] thiazol-5-yl} -benzo [1,3] dioxol-5-yl- Methanone,
p) {4-amino-2- [4- (4-cyclopentyl-piperazin-1-yl) -phenylamino] thiazol-5-yl}-(2,3-dihydro-benzo [1,4 ] Dioxin-6-yl) -methanone,
q) {4-amino-2- [4- (4-cyclopropylmethyl-piperazin-1-yl) -phenylamino] thiazol-5-yl} -benzo [1,3] dioxol-5-yl -Metanon, and
r) {4-amino-2- [4- (4-cyclopropylmethyl-piperazin-1-yl) -phenylamino] thiazol-5-yl}-(2,3-dihydro-benzo [1, 4] dioxin-6-yl) -methanone.
[9" claim-type="Currently amended] 8. The compound of claim 1, wherein R ⁵ is selected from the group consisting of H and F. 9.
[10" claim-type="Currently amended] The compound of claim 9, wherein the compound is selected from the group consisting of:
a) [4-amino-2-[[4- [4- (2-hydroxyethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (3,5-difluorophenyl) Methanone,
b) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] thiazol-5-yl}-(3,5-difluoro-phenyl) -meta Paddy Field,
c) (4-amino-2- {4- [4- (2-methoxy-ethyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3,5-di Fluoro-phenyl) -methanone,
d) {4-amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3,5-difluoro-phenyl) -meta Rice fields, and
e) [4-amino-2- (4-piperazin-1-yl-phenylamino) -thiazol-5-yl]-(3,4,5-trifluorophenyl) -methanone.
[11" claim-type="Currently amended] 8. A compound according to any one of claims 1 to 7, wherein R ⁵ is H.
[12" claim-type="Currently amended] 8. A compound according to any one of claims 1 to 7, wherein at least one of R ³ , R ⁴ , and R ⁵ is halogen.
[13" claim-type="Currently amended] 13. A compound according to claim 11 or 12, which is selected from the group consisting of:
a) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (3,4-dichlorophenyl) methanone,
b) [4-amino-2-[[4- [4- (2-hydroxyethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (3-fluorophenyl) methanone,
c) [4-amino-2-[[4- (1-piperazinyl) phenyl] amino] -5-thiazolyl] (3-fluorophenyl) methanone,
d) (4-amino-2- {4- [4- (3-hydroxy-propyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3-fluoro- Phenyl) -methanone,
e) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-methoxy-phenyl) -methanone,
f) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3,4,5-trifluoro-phenyl ) -Methanone,
g) (4-amino-2- {4- [4- (2-methoxy-ethyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3,4-di Fluoro-phenyl) -methanone,
h) (4-amino-2- {4- [4- (2-hydroxy-ethyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3-fluoro- Phenyl) -methanone,
i) {4-Amino-2- [4- (4-sec-butyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-fluoro-phenyl) -methanone ,
j) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-fluoro-phenyl) -methanone, And
k) {4-Amino-2- [4- (4-cyclopropylmethyl-piperazin-1-yl) -phenylamino] thiazol-5-yl}-(3-fluoro-phenyl) -methanone.
[14" claim-type="Currently amended] 8. The compound of claim 1, wherein at least one of R ³ , R ⁴ , and R ⁵ is selected from the group consisting of OR ¹¹ , OCF ₃ , and OCHF _{2. 9} .
[15" claim-type="Currently amended] The compound according to any one of claims 1 to 14, which is selected from the group consisting of:
a) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl]-(4-hydroxyphenyl) methanone,
b) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (4-methoxyphenyl) methanone,
c) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (3-fluoro-4-methoxyphenyl) methanone,
d) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (3-methoxyphenyl) methanone,
e) [4-amino-2-[[3-fluoro-4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (3,5-dimethoxyphenyl) methanone ,
f) [4-amino-2-[[3-fluoro-4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (3-methoxyphenyl) methanone,
g) [4-amino-2-[[4- [4- (1-methylethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (3-methoxyphenyl) methanone,
h) [4-amino-2-[[4- [4- (1-methylethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (3,5-dimethoxyphenyl) methanone ,
i) [4-amino-2-[[4- [4- (2-hydroxyethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (3-methoxyphenyl) methanone,
j) [4-amino-2-[[4- (1-piperazinyl) phenyl] amino) -5-thiazolyl] (3-fluoro-4-methoxyphenyl) methanone,
k) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-methoxy-phenyl) -methanone,
l) (4-amino-2- {4- [4- (3-hydroxy-propyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3-methoxy- Phenyl) -methanone,
m) (4-amino-2- {4- [4- (2-methoxy-ethyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3-fluoro- 4-methoxy-phenyl) -methanone,
n) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(4-fluoro-3-methoxy-phenyl ) -Methanone,
o) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-fluoro-4-methoxy-phenyl ) -Methanone,
p) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(4-difluoromethoxy-phenyl) -meta Paddy Field,
q) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-trifluoromethoxy-phenyl) -meta Paddy Field,
r) (4-amino-2- {4- [4- (2-methoxy-ethyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3-methoxy- Phenyl) -methanone,
s) {4-amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3,5-difluoro-4-methoxy -Phenyl) -methanone,
t) {4-amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(4-fluoro-3-methoxy-phenyl) -Methanone,
u) {4-Amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-trifluoromethoxy-phenyl) -methanone ,
v) [4-amino-2- (4-piperazin-1-yl-phenylamino) -thiazol-5-yl]-(3,5-difluoro-4-methoxy-phenyl) -methanone ,
w) [4-amino-2- (4-piperazin-1-yl-phenylamino) -thiazol-5-yl]-(4-fluoro-3-methoxy-phenyl) -methanone,
x) [4-amino-2- (4-piperazin-1-yl-phenylamino) -thiazol-5-yl]-(3-trifluoromethoxy-phenyl) -methanone,
y) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-trifluoromethoxy-phenyl) -meta Paddy Field,
z) {4-Amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-difluoromethoxy-phenyl) -meta Paddy Field,
aa) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-hydroxy-phenyl) -methanone,
bb) {4-amino-2- [4- (4-isobutyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-hydroxy-phenyl) -methanone,
cc) [4-amino-2- (4-piperazin-1-yl-phenylamino) -thiazol-5-yl]-(3-difluoromethoxy-phenyl) -methanone,
dd) {4-amino-2- [4- (4-cyclopropylmethyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-difluoromethoxy-phenyl)- Methanone,
ee) {4-amino-2- [4- (4-cyclopropylmethyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-methoxy-phenyl) -methanone ,
ff) {4-Amino-2- [4- (4-cyclopropylmethyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-fluoro-4-hydroxy- Phenyl) -methanone,
gg) {4-amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-difluoromethoxy-phenyl) -methanone ,
hh) {4-amino-2- [4- (4-cyclopropylmethyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-fluoro-4-methoxy- Phenyl) -methanone, and
ii) {4-amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-hydroxy-phenyl) -methanone.
[16" claim-type="Currently amended] 8. The compound according to claim 1, wherein each of R ³ , R ⁴ and R ⁵ is independently selected from NO ₂ , NHSO ₂ R ⁴ and NR ¹² R ¹³ .
[17" claim-type="Currently amended] The compound of claim 16, wherein the compound is selected from the group consisting of:
a) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [4- (1-pyrrolidinyl) phenyl] methanone,
b) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [4- (1-piperidinyl) phenyl] methanone,
c) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] -amino] -5-thiazolyl] [4- (4-morpholinyl) with acetate salts Phenyl] methanone,
d) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (3-nitrophenyl) methanone,
e) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [4- (diethylamino) phenyl] methanone,
f) N- [4-[[4-amino-2-[[4- [4- (1-methylethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] carbonyl] phenyl] Acetamide,
g) [4-amino-2-[[4- [4- (1-methylethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] [4- (diethylamino) phenyl] meta Paddy Field,
h) 1-acetyl-4- [4-[[4-amino-5- [4- (diethylamino) benzoyl] -2-thiazolyl] amino] phenyl] piperazine, trifluoroacetate salt,
i) [4-amino-2-[[4- [4- (2-hydroxyethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] [4- (1-pyrrolidinyl) Phenyl] methanone,
j) [4-amino-2-[[4- (1-piperazinyl) phenyl] amino) -5-thiazolyl] [4- (1-pyrrolidinyl) phenyl] methanone,
k) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [4- (2-hydroxyethyl) amino-3-nitrophenyl ] Methanone,
l) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4- (1-pyrrolidinyl) phenyl] Methanone,
m) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4- (4-morpholinyl) phenyl] Methanone,
n) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4-[(2-methoxyethyl) amino ] Phenyl] methanone,
o) racemic [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4- [3- (hydroxymethyl ) -1-piperidinyl] phenyl] methanone,
p) racemic [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4- (2-methyl-1- Pyrrolidinyl) phenyl] methanone,
q) (R)-[4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4-[[1- ( Hydroxymethyl) -3-methylbutyl] amino] phenyl] methanone,
r) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-nitro-4- (4-hydroxy-1-pipepe Ridinyl) phenyl] methanone,
s) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [3-amino-4- (4-pyrrolidinyl) phenyl] Methanone,
t) (R)-[3-amino-4-[[1- (hydroxymethyl) -3-methylbutyl] amino] phenyl] [4-amino-2-[[4- (4-methyl-1- Piperazinyl) phenyl] amino] -5-thiazolyl] methanone,
u) [4-amino-2-[[3-fluoro-4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] [4- (1-pyrrolidinyl) phenyl ] Methanone,
v) (4-amino-2- {4- [4- (3-hydroxy-propyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3-nitro-phenyl ) -Methanone,
w) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] thiazol-5-yl}-(4-piperidin-1-yl-phenyl) -Metanon, and
x) {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] thiazol-5-yl}-(4-morpholin-4-yl-phenyl)- Metanon.
[18" claim-type="Currently amended] 8. The compound of claim 1, wherein at least one of R ³ , R ^4, and R ⁵ is CN. 9.
[19" claim-type="Currently amended] 19. The compound of claim 18, wherein the compound is selected from the group consisting of:
a) 3- (4-amino-2- {4- [4- (3-hydroxy-propyl) -piperazin-1-yl] -phenylamino} -thiazole-5-carbonyl) -benzonitrile,
b) 3- {4-amino-2- [4- (4-isopropyl-piperazin-1-yl) -phenylamino] -thiazole-5-carbonyl} -benzonitrile, and
c) 3- {4-amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazole-5-carbonyl} -benzonitrile.
[20" claim-type="Currently amended] 20. The compound of any one of claims 1 to 19, wherein each one of R ³ , R ⁴ and R ⁵ is independently selected from H and lower alkyl.
[21" claim-type="Currently amended] The compound of claim 20, wherein the compound is selected from the group consisting of:
a) (4-amino-2- {4- [4- (3-hydroxy-propyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl) -m-tolyl-methanone ,
b) (4-amino-2- {4- [4- (3-hydroxy-propyl) -piperazin-1-yl] -phenylamino} -thiazol-5-yl)-(3-ethyl-phenyl ) -Methanone,
c) {4-amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl}-(3-ethyl-phenyl) -methanone, and
d) {4-amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl} -m-tolyl-methanone.
[22" claim-type="Currently amended] 22. The compound of any one of claims 1 to 21, wherein at least one of R ³ , R ⁴ and R ⁵ is selected from S-lower alkyl.
[23" claim-type="Currently amended] The compound of claim 27, wherein the compound is selected from the group consisting of:
a) {4-amino-2- [4- (4-methyl-piperazin-1-yl) -phenylamino] -thiazol-5-yl} (3-methylsulfanyl-phenyl) -methanone,
b) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (2,3-dihydro-1,4-benzodioxin- 6-day) methanone, and
c) [4-amino-2-[[4- [4- (1-methylethyl) -1-piperazinyl] phenyl] amino] -5-thiazolyl] (3-methoxyphenyl) methanone.
[24" claim-type="Currently amended] A pharmaceutical composition comprising as an active ingredient an effective amount of a compound according to any one of claims 1 to 23 and a pharmaceutically acceptable carrier or excipient.
[25" claim-type="Currently amended] A method of treating cancer, comprising administering to a subject in need thereof a therapeutically effective amount of a compound according to any one of claims 1 to 23.
[26" claim-type="Currently amended] The method of claim 25, wherein the cancer is a solid tumor.
[27" claim-type="Currently amended] 27. The method of claim 25 or 26, wherein the solid tumor is a breast, colon, lung or prostate tumor.
[28" claim-type="Currently amended] An intermediate compound for the preparation of a compound according to any one of claims 1 to 23, selected from the group consisting of:
a) 1- (2-fluoro-4-isothiocyanatophenyl) -4-methylpiperazine,
b) 1- (1-methylethyl) -4- (4-nitrophenylpiperazine),
c) 4- [4- (1-methylethyl) -1-piperazinyl] benzeneamine,
d) 1- (4-isothiocyanatophenyl) -4- (1-methylethyl) piperazine,
e) 4- (2-hydroxyethyl) -1- (4-isothiocyanatophenyl) piperazine,
f) 1- (4-isothiocyanatophenyl) -4- (2-methoxy-ethyl) piperazine,
g) 1- (4-isothiocyanatophenyl) -4- (1-methylpropyl) piperazine,
h) 4-cyclopentyl-1- (4-isothiocyanatophenyl) piperazine,
i) 1- (4-isothiocyanatophenyl) -4- (2-methylpropyl) piperazine,
j) 4- (3-hydroxypropyl) -1- (4-isothiocyanatophenyl) piperazine,
k) 2-bromo-1- (3,4,5-trifluoro-phenyl) ethanone,
1) 2-bromo-1- (4-fluoro-3-methoxy-phenyl) ethanone,
m) 2-bromo-1- (4-fluoro-3-methoxy-phenyl) ethanone,
n) 2-bromo-1- (3-difluoromethoxy-phenyl) ethanone,
o) carbamimidothioic acid [[4- (4-methyl-1-piperazinyl) phenylamino] thioxomethyl], bound to a polymer;
p) carbamimidothioic acid [[3-fluoro-4- (4-methyl-1-piperazinyl) phenylamino] thioxomethyl], bound to a polymer;
q) carbamimidothioic acid [[4- [4- (1-methylethyl) -1-piperazinyl] phenylamino] thioxomethyl], bound to a polymer;
r) carbamimidothioic acid [[4- (4-acetyl-1-piperazinyl) phenylamino] thioxomethyl], bound to a polymer;
s) carbamimidothioic acid [[4- [4- (2-hydroxyethyl) -1-piperazinyl] phenylamino] thioxomethyl], bound to a polymer;
t) carbamimidothioic acid [[4- [4-[(1,1-dimethylethoxy) carbonyl] -1-piperazinyl] phenylamino] thioxomethyl], bound to a polymer;
u) carbamimidothioic acid [[4- [4- (2-methoxyethyl) -1-piperazinyl] phenylamino] thioxomethyl], bound to a polymer;
v) carbamimidothioic acid [[4- [4- (1-methylpropyl) -1-piperazinyl] phenylamino] thioxomethyl], bound to a polymer;
w) carbamimidothioic acid [[4- (4-cyclopentyl-1-piperazinyl) phenylamino] thioxomethyl], bound to a polymer;
x) carbamimidothioic acid [[4- [4- (2-methylpropyl) -1-piperazinyl] phenylamino] thioxomethyl], bound to a polymer;
y) carbamimidothioic acid [[4- [4- (3-hydroxypropyl) -1-piperazinyl] phenylamino] thioxomethyl], bound to a polymer;
z) [4-amino-2-[[4- (4-methyl-1-piperazinyl) phenyl] amino] -5-thiazolyl] (4-chloro-3-nitrophenyl) methanone,
aa) 4- [4-[[4-amino-5- (3-fluorobenzoyl) -2-thiazolyl] amino] phenyl] -1-piperazinecarboxylic acid, 1,1-dimethylethyl ester,
bb) 4- [4-[[4-amino-5- [4- (1-pyrrolidinyl) benzoyl] -2-thiazolyl] amino] phenyl] -1-piperazinecarboxylic acid, 1,1- Dimethylethyl ester, and
cc) 4- [4-[[4-amino-5- (3-fluoro-4-methoxybenzoyl) -2-thiazolyl] amino] phenyl] -1-piperazinecarboxylic acid, 1,1- Dimethylethyl ester.
[29" claim-type="Currently amended] Use of a compound according to any one of claims 1 to 23 for the manufacture of a medicament.
[30" claim-type="Currently amended] 41. Use according to claim 40 for the manufacture of a medicament for the treatment of cancer.
[31" claim-type="Currently amended] 42. Use according to claim 40 or 41 for the manufacture of a medicament for the treatment of breast, colon, lung or prostate tumors.
[32" claim-type="Currently amended] 24. A process for the preparation of a compound according to any one of claims 1 to 23, comprising alkylation and cyclization of a halomethylketone, such as bromomethylketone, with the following a) or b):
a) thiourea derivative of formula
[Formula 4]
b) Thiourea Derivatives of Formula 4A
[Formula 4A]
[33" claim-type="Currently amended] The compound according to claim 1, which is obtained by the process according to claim 32.
[34" claim-type="Currently amended] 24. A compound according to any one of claims 1 to 23, for use according to any one of claims 29 to 31.
[35" claim-type="Currently amended] 35. Novel compounds, pharmaceutical compositions, methods, uses, and methods of preparation according to any one of claims 1 to 34.

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同族专利:

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DE60208661D1|2006-04-06|

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US20040082595A1|2004-04-29|

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DE60208661T2|2006-08-17|

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KR100530977B1|2006-01-11|

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JP2004517895A|2004-06-17|

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ES2255606T3|2006-07-01|

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PA8537401A1|2002-09-30|

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WO2002057261A3|2002-10-17|

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JP4173736B2|2008-10-29|

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CN1487927A|2004-04-07|

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EP1358169B1|2006-01-11|

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US7094896B2|2006-08-22|

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US6756374B2|2004-06-29|

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AR035532A1|2004-06-02|

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US7105670B2|2006-09-12|

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US20020151554A1|2002-10-17|

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AU2002242687B2|2007-05-10|

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CA2434296A1|2002-07-25|

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US20040087594A1|2004-05-06|

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PE20020915A1|2002-10-19|

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EP1358169A2|2003-11-05|

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BR0206496A|2004-02-10|

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MXPA03006420A|2003-10-15|

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WO2002057261A2|2002-07-25|

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PA8537501A1|2002-08-29|

引用文献:

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公开号 | 申请日 | 公开日 | 申请人 | 专利标题

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法律状态:
2001-01-22|Priority to US26331501P

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2001-01-22|Priority to US60/263,315

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2001-10-03|Priority to US32680701P

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2001-10-03|Priority to US60/326,807

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2002-01-18|Application filed by 에프. 호프만-라 로슈 아게

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2002-01-18|Priority to PCT/EP2002/000506

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2003-08-25|Publication of KR20030069222A

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2006-01-11|Application granted

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2006-01-11|Publication of KR100530977B1

优先权:

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申请号 | 申请日 | 专利标题

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US26331501P| true| 2001-01-22|2001-01-22|

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US60/263,315|2001-01-22|

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US32680701P| true| 2001-10-03|2001-10-03|

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US60/326,807|2001-10-03|

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PCT/EP2002/000506|WO2002057261A2|2001-01-22|2002-01-18|Diaminothiazoles and their use as inhibitors of cyclin-dependent kinase|