• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    The present invention relates to an additive printing equipment and method comprising an orientable energy excitation means for producing a point interaction with a fluid covering a blade (2), in order to cause a directed jet towards a target (11). ), said fluid being constituted by a liquid vector containing inhomogeneities, characterized in that • said fluid forms a liquid film (4) with a thickness of less than 500 μm, • on a blade (2) having at least one zone ( 3) allowing interaction with the laser in which at least one inlet (7) opens, said interaction zone (3) opening into at least one outlet (9) • the equipment further comprising means for circulating the fluid between said inlet (7) and said outlet (9).
    公开号:FR3063931A1
    申请号:FR1752131
    申请日:2017-03-15
    公开日:2018-09-21
    发明作者:Fabien Guillemot；Bertrand Viellerobe；Jerome Bouter；Evarzeg Le Bouffant；Romain Vaucelle
    申请人:Poietis；
    IPC主号:
    专利说明:

    Field of the invention
    The present invention relates to the field of three-dimensional additive printing assisted by laser and more particularly, but not limited to, bioprinting.
    Three-dimensional additive printing assisted by laser consists in projecting particles towards a target by a jet produced by the local vaporization of a substrate providing kinetic energy to a transferable particle or an aggregate of particles contained in a carrier fluid.
    The transferable particles can come from a pulverulent material carried by a liquid substrate, or a liquid transferable material.
    They can be metallic nature, of inorganic compounds, or polymers or of biomaterials.They can also be made up of of
    biological particles, for example living cells.
    The present invention relates to the field of bioprinting carried out by a computer-assisted transfer process for modeling and assembling living and non-living materials with a prescribed organization in 2D or 3D in order to produce structures. three-dimensional, including bioengineering structures used in regenerative medicine, pharmacology and for cell biology studies.
    Tissue engineering aims to design and develop biologically suitable replacement solutions to replace, restore or maintain the functions of a native tissue, or even an organ. An example is described in the article Griffith, L. G., & Naughton, G. (2002). Tissue engineering— current challenges and expanding opportunities. Science, 295 (5557), 1009-1014.
    To overcome these limitations, the printing of biological elements, more commonly known as bio-printing, began to be imagined, as explained in the articles Klebe, R. (1988). Cytoscribing: A Method for Microquering Cells and the Construction of Two- and Three-Dimensional Synthetic Tissues. Experimental Cell Research, 179 (2): 362-373.
    And Klebe, R., Thomas, C., Grant, G., Grant, A. and Gosh, P. (1994). Cytoscription: Computer controlled micropositioning of cell adhesion proteins and cells. Methods in Cell Science, 16 (3): 189-192.
    State of the art
    Patent WO2016097619 describing a method for printing at least one ink is known in the state of the art, said method comprising a step of focusing a laser beam so as to generate a cavity in an ink film, a step of forming at least one ink droplet from a free surface of the ink film and a step of depositing said droplet on a surface for depositing a receiving substrate positioned at a given distance (L ) of the film, characterized in that the laser beam is oriented in the opposite direction with respect to the gravitational force (G), the free surface of the film being oriented upwards in the direction of the deposition surface placed above the film of ink.
    Patent WO2014061024 is also known concerning a system for performing a laser-induced forward transfer (LIFT) without substrate and / or with local donor. This system includes a reservoir comprising at least one opening. An energy source configured to deliver energy to a donor material within said reservoir. This system allows the deposition of material by laser induced forward transfer without any need for a donor substrate. The invention also relates to substrate-induced and local donor laser-induced forward transfer methods.
    Disadvantages of the prior art
    In the solutions of the prior art providing for a blade coated with a fluid containing the particles to be transferred, as it is necessary to replace the blade after each sequence, this does not allow good control of the characteristics of the film containing the transferable elements, in particular the volume of the fluid, the spreading of the fluid on the surface of the blade, the homogeneity and the evolution over time due to the phenomena of drying, evaporation, evolution of living particles, ... Thus, the main drawback of the solutions of the prior art concerns both a lack of reproducibility of the prints and the need for frequent recourse to manipulations, reducing productivity.
    In the solutions providing for a tank or a reservoir containing a fluid, the problems which arise relate to the control of the generation of the jet at the air-liquid interface, because the materials to be transferred tend to sediment and thus to be found far from target. These solutions do not therefore allow the particles contained in a fluid to be transferred under reproducible conditions. This is a generic drawback known also for bio extrusion or inkjet solutions.
    In general, the solutions of the prior art are not suitable for an industrial process for printing liquid media containing particles, due to the difficulty of precisely targeting the particles contained in the fluid and the need to change regularly the support. These changes of support impose manipulations resulting in the lack of reproducibility noted.
    A secondary problem which the invention aims to remedy by some of its variants relates to the abandonment of the interaction of the laser with a sacrificial layer, for example a coating of gold.
    For the solutions of the prior art using a sacrificial layer, the jet formed by a laser exciting this layer causes the transfer of material from this layer which can cause toxicity problems, projection of particles other than the particles to be to transfer.
    They also involve the local destruction of the substrate during each shot, which creates inhomogeneities and imposes a repeated change of the substrate.
    Solution provided by the invention
    Inhomogeneity of the bio-ink film is understood to mean, within the meaning of the present patent, any area of the film having specific local characteristics in terms of composition: particles, biochemical species (growth factor, molecules, ions), biomaterials.
    The terms “inhomogeneous zone”, “local variations in composition”, “zone of specific composition” have the same technical meaning within the meaning of this patent.
    The solution consists in making the inhomogeneities of the film of fluid positioned in the laser interaction zone homogeneous in thickness and in density by volume during the laser-assisted three-dimensional additive printing. It also consists in allowing the filling, repeatedly and controlled by the fluid, of said interaction zone. Such a solution, on the other hand, requires the installation of a laser printing process that does not use a sacrificial layer for the generation of the jets of material, which in fact involves a laser-material interaction which takes place directly in the fluid . Thus, the process for generating the cavitation bubble and then the material jet will be completely different from the general prior art.
    The advantages of this solution are numerous:
    - it allows the inhomogeneous fluid to be brought in a controlled and reproducible manner to the interaction zone, avoiding any manipulation (pipetting, spreading, cleaning, etc.). It therefore makes the process more secure and more reliable.
    - it modulates the composition of the ink by mixing several liquids (chemical species, liquid biomaterials, ...) and several types of particles (cells, biomaterials, ...) in the same type of ink.
    - it allows the use of a controllable fluidic system which continuously or pseudo-continuously recharges the interaction zone, thus making it possible to gain productivity in printing.
    - It makes it possible to obtain a film of homogeneous fluid in thickness on the surface of the interaction zone which aims to make the printing much more reproducible and homogeneous at the level of the droplets printed on the receiving surface.
    - it allows the adjustment of the film thickness thanks to optimized sets of parameters (flow rate, section, shape ...) of the fluidic system. Thus, the height of the material jets can be adjusted in this way, which can be very advantageous for printing on non-planar surfaces.
    Furthermore, the invention allows the use of imaging means correlated to laser shots in order to target inhomogeneities in the fluid in a controlled manner. To do this, the interaction zone must rest on a transparent material both for the laser and for image acquisition means.
    Even if this solution is compatible with laser printing based on the use of a sacrificial layer (typically a metallic layer of gold or silver), it is preferably intended for laser printing without the use of a layer. sacrificial. Such a solution must therefore ensure the creation of reproducible and repeatable jets in the field during a direct interaction between the laser and the fluid containing the inhomogeneities. To do this, a certain number of printing parameters, listed below, are necessary because the generation of the jets is very difficult to obtain in this printing condition without sacrificial layer:
    - The laser preferably emits short pulses in picosecond or femtosecond regime with an energy level between 1 to 40 microjoules, and preferably 5 and 20 micro joules in order to optimize the generation of laser plasma in the fluid. On reading the exemplary embodiments which will be described in this document, proof of these performances will be provided.
    - The laser preferably emits pulses in the near IR range to avoid any ionizing effect on the cells while being sufficiently absorbable by the medium. To optimize this last parameter, it would be entirely possible to use a laser in the UV or the IR medium, or even in the visible in order to maximize the rate of absorption by the medium.
    - Measurements of characteristic properties of the fluid present in the interaction zone are carried out (density, viscosity, film thickness, etc.) in order to modulate or optimize the laser parameters and to make the impression as homogeneous as possible.
    - the images of inhomogeneities in the fluid make it possible to target specific zones (number or type of particles), which again makes it possible to make the printing homogeneous and above all conform to the digital printing file since the number of inhomogeneities printed can be directly controlled by means of these imaging means or more generally of characterization controlled by a computer for the interpretation of the acquired data.
    In such a context, there is no longer any problem linked to the printing of debris from the sacrificial layer towards the printing substrate, thus ensuring a higher viability of the cells in the context of bioprinting.
    Solution provided by the invention
    The invention relates, according to its most general meaning, to additive printing equipment comprising an orientable energy excitation means for producing a specific interaction with a fluid covering a blade, in order to cause a jet directed towards a target, said fluid consisting of a liquid carrier containing transferable particles or of a transferable liquid biomaterial, characterized in that:
    • said fluid forms a liquid film with a thickness of less than 500 μm, • on a slide having at least one zone allowing interaction with the laser into which opens at least one entry, said interaction zone opening into at least one outlet, said interaction zone having an opening whose cross section is at least 3 times greater than the median size of the inhomogeneities present in the fluid • the equipment further comprising means for circulation of the fluid between said inlet (7) and said exit.
    According to particular variants of implementation of the equipment according to the invention,
    • the thickness of said film is between 50 and 100 pm • the thickness of said film is between 20 and 100 pm • the thickness of said film is between 3 and 10 time nominal size said transferable particles • the thickness of said film is between 5 and 10 time
    the nominal size of said transferable particles • the surface of said interaction zone is greater than 0.05 mm 2 • said entry opens into a lateral part of said interaction zone • said interaction zone has a peripheral part opening laterally into said outlet • said inlet and said outlet are constituted by tubular channels connected to the connection zone, the longitudinal axis of each of said tubular channels forming with the transverse plane of the interaction zone between 15 ° and 35 ° • said means for circulating the fluid between said inlet and said outlet comprise means for controlling the injection flow rate (or the positive pressure exerted on the fluid) and the suction flow rate to control the flow rate of the fluid (or negative pressure exerted on the fluid) in the interaction zone • said means for controlling the injection rate and the suction rate are as used to measure the thickness of the film, to control the thickness of the film in the interaction zone • the equipment comprises a plurality of interaction zones each comprising an inlet and an outlet • at least two of said zones interaction have common inputs and / or outputs • said energetic excitation means is constituted by a laser • the interaction zone is transparent in the wavelength band of the laser and of the imagery and does not have of sacrificial layer • said fluid is charged with an absorbent pigment in the wavelength of emission of the laser • the equipment comprises means of imaging of the interaction zone for the piloting of the laser according to the density of particles • said laser emits pulses in picosecond or femtosecond regime with an energy level between 20 and 40 microjoules • said laser emits pulses in picosecond or femtosecond regime with a level of energy between 5 and 20 microjoules, the energy level per pulse being controlled by a computer as a function of the measurement result of characteristics of the fluid present in the interaction zone, said measurements comprising the particle density, and / or the viscosity, and / or the thickness of the film • said energy excitation means consists of an acoustic wave generator • the equipment comprises means for imaging the interaction zone and for selecting the type of particle to transfer.
    The invention also relates to a method of additive printing by equipment comprising a means of energetic excitation ίο to produce a punctual interaction with a fluid covering a blade, in order to cause a jet oriented towards a target, said fluid. being constituted by a liquid vector containing transferable inhomogeneities (particles, or biomaterials or chemical species) or by a transferable liquid biomaterial, characterized in that said fluid forms a liquid film with a thickness of less than 500 µm circulating between a conduit inlet (7) and an outlet duct of a blade having at least one area allowing interaction with the laser, and into which opens at least one inlet (7).
    According to a particular variant, the energy level per pulse is controlled by a computer as a function of the measurement result of characteristics of the fluid present in the interaction zone, said measurements comprising the particle density, and / or the viscosity, and / or the thickness of the film.
    Detailed description of a nonlimiting example of
    The invention
    The present invention will be better understood on reading the detailed description of a nonlimiting example of the invention which follows, referring to the accompanying drawings in which:
    - Figure 1 shows a schematic sectional view of equipment according to the invention
    - Figure 2 shows a schematic view of equipment according to the invention with the optical system.
    - Figure 3 shows a schematic top view of a blade for equipment according to the invention
    - Figure 4 shows a 3D view of different variants of the equipment according to the invention implemented experimentally
    - Figure 5 shows a 3D view of different variants of the equipment according to the invention comprising a groove for making the image of the ink film by the edge
    - Figure 6 shows a 3D view of a variant of the equipment according to the invention as well as images of ink films obtained in this configuration
    - Figure 7 shows a 3D view of a multi-cavity variant of the equipment according to the invention
    - Figure 8 shows a series of explanatory diagrams of the laser-matter interaction process without sacrificial layer implemented in the equipment according to the invention
    - Figure 9 represents a series of photographs showing the jet of material generated by laser interaction - material without sacrificial layer within the equipment according to the invention; Figure 10 represents an image taken under the microscope of a printed field of droplets d in the equipment according to the invention in static mode (without continuous recharging)
    FIG. 11 represents an image taken under the microscope of a printed field of droplets containing microbeads within the equipment according to the invention in static mode (without continuous recharging) FIG. 12 represents an image taken under the microscope of a printed field of droplets containing cells within the equipment according to the invention in static mode (without continuous recharging) FIG. 13 represents an image taken under the microscope of a printed field of water droplets produced within 1 ' equipment according to the invention in dynamic mode (with continuous recharging) FIG. 14 represents an image taken under the microscope of a printed field of droplets containing cells as well as melanin (absorber) within the equipment according to the invention in static mode (without continuous recharging)
    Description of a first variant of an equipment according to
    The invention
    FIG. 1 represents a first alternative embodiment of an equipment, implementing an excitation by a focused laser beam generating a pulse (1).
    A blade (2) made of glass or transparent material defines a cavity (3) in which a carrier fluid (4) containing transferable particles (5) circulates.
    The depth of this cavity is less than 500 μτα and preferably from 50 to 100 μm thick, thus avoiding sedimentation phenomena in the cavity (3).
    This cavity (3) is formed by molding, machining, blowing (glass) or even by 3D printing (FDM, SLS, SLA, DLP, DMLS, EBM, CLIP, MultiJet, ...) and has a circular or rectangular section , or oval, or other geometric shapes. Its transverse surface (6) defines a working area which can be scanned by the laser beam (1) and displayed on a sensor via an optical back beam.
    The carrier fluid (4), pushed by a pumping system (15), enters the cavity (3) through an inlet opening (7) connected to a supply conduit (8) itself connected to a reservoir d supply (14), and is evacuated via an outlet orifice (9) to a conduit (10) for evacuation and / or aspiration.
    The evacuation and / or suction pipe (10) opens into a recovery tank (13) containing the carrier fluid (4) loaded with transferable particles (5). A pump (15) circulates the carrier fluid (4) loaded with transferable particles (5). The supply tank (14) and the recovery tank (13) can be separated or else form a single tank if it is desired to recirculate the same fluid in the system several times. In this configuration, the advantage is to maximize the number of particles printed in the circulating fluid.
    Optionally, the system includes several sets of reservoirs (14 and 13), each containing a carrier fluid charged with inhomogeneities of different natures. A valve makes it possible to select one of the reservoirs, to allow the deposit of particles of different natures and the formation of differentiated layers on the target (11).
    The flow rate of carrier fluid (4) is adjusted to ensure the displacement of the working area of the transferable particles (5) at a speed allowing selection by appropriate means (imaging, spectroscopy, ...) and activating those which are selected. by laser shooting.
    The target (11) is movable in an X, Y plane parallel to the bottom (6) of the cavity (3) to determine the point of deposit of the transferred particle (12) and possibly in a perpendicular direction, to adjust the distance traveled by the particle (5) to be transferred. In this case, it is possible to modulate the size of the droplets deposited on the print receiving substrate.
    Figure 2 shows a sectional view of the device completed with the optical system.
    This optical system is constituted by two angularly oscillating mirrors (of the galvanometer type) (20), making it possible to scan the laser shooting area, and by a first optical unit (21) consisting of a scanning lens, of the F- lens type. Theta, used to form a laser spot with the smallest and most constant diameter on the work surface. This first optical unit (21) is constituted in a known manner by a system of several lenses.
    Upstream of the scanning mirrors (20), the optical system comprises a laser source (22) whose beam is returned to the scanning mirrors (20) by a dichroic mirror (23).
    A second optical unit (24) forms an image of the working area (25) by the back beam passing through the dichroic mirror (23), on a sensor (26).
    Description of a second variant of an equipment according to the invention
    Figure 3 shows a top view of a variant of a blade (2) according to the invention.
    It comprises three circuits formed by three cavities (30, 31, 32) parallel, each extending between a supply duct (respectively 33 to 35) and a discharge duct respectively (36 to 38).
    Each circuit ensures the circulation of a carrier fluid containing transferable inhomogeneities respectively (39 to 41) of potentially different natures. Indeed, either they are of the same nature which could allow printing on larger fields in size or else speed up the printing process (productivity gain), or they are of different nature which could allow to manufacture complex and personalized objects (gain on the range of manufacturable objects), it is the "multicolor" side brought by this kind of architecture.
    To select one of the cavities, the blade (2) can be moved mechanically in a direction perpendicular to the main axis of the three cavities, or else the scanning of the laser beam makes it possible to cover the entire blade (2).
    Description of implementations of different equipment according to the invention
    Figure 4 shows a view of 3 possible architectures of the equipment. FIG. 4.a represents a view where the supply (8) and evacuation (10) conduits are parallel to the blade (2) while FIG. 4.b illustrates a situation where the conduits arrive at an angle by ratio to the blade which can be between 0 and 90 °. The advantage of one or the other of the solutions lies in the ability to manage the flow rates, the dead volumes or even the angles in order to avoid any problem of blockage or continuity in the flow of the fluid (4) and to guarantee at the same time obtaining a film of homogeneous fluid in the open cavity (3) (circular part in the figures where the laser shots are focused). Figure 4.c is a variant of the previous solutions where glass strips were placed at the inlets (7) and outlets (9) of the fluid (4) to orient / guide its flow in the form of a homogeneous film in the cavity (3). The architectures illustrated here are not exhaustive. Indeed, the shape, the positioning and the angle of the conduits, the size and the shape of the cavity and the blade, the materials used, the shape and the positioning of the inlet (7) and outlet orifices may differ. examples illustrated here.
    FIG. 5 represents a view of 3 possible architectures of the equipment close to those presented in FIG. 4. They all have in common to present in this new configuration a groove on the upper part whose height corresponds to that of the cavity (3) and whose positioning intersects the open area of the cavity (3). The advantage of such an opening is to allow observation of the film by means of visualization (imagery) placed perpendicularly to the equipment. Thus, it is possible to follow the evolution of the film over time, which makes it possible to best adapt the energy of the laser shots to the real thickness of the film during printing or, conversely, to adapt the thickness of the film. film with the energy of laser shooting. Again, the architectures illustrated here are not exhaustive. Again, the shape, positioning and angle of the conduits, the size and shape of the cavity and the blade, the materials used, the shape and positioning of the inlet (7) and outlet orifices, the shape, size and positioning of the grooves may differ from the examples illustrated here.
    Configuration architecture.
    FIG. 6 presents results obtained on one of the variants of the equipment according to the invention, initially illustrated in FIG. 5.e. In Figure 6.h, a transparency view allows internal lighting of the equipment in this
    The supply and exhaust ducts arrive at an angle to the cavity (3) and to the blade (2). The link between the conduits and the cavity area is made by small diameter pipes to be compatible with the desired film thicknesses of less than 500μιη. However, it is not necessary that the cross section of these conduits is too small in order to avoid any phenomenon comparable to those observed in nozzle systems (orifices) which are easily blocked and which bring significant mechanical stress to the cells, thus impacting their viability over time. It is for this reason that the conduits will preferably have a diameter greater than 200 μm, or at least 10 times the average size of the cells or particles printable by this equipment. We can notice that the equipment is pierced in its center by a hole (cylinder) allowing the laser to be focused in the cavity by the way (2), transparent at its wavelength. On a zoomed view of the cavity (3) located above the blade
    at through the blade the figure 6. i, a top of the blade
    (2) on which the fluid is sent (4) makes it possible to show that the inlet (7) and outlet (7 and 9) respectively are partly formed by the supply and discharge conduits respectively (8 and 10) and by the glass strips placed on the top of the equipment. The association of the conduits and the lamellae allows the fluid (4) to be directed properly onto the blade (2). Images showing that it is possible to obtain thin films are represented in photographs 6.j, 6.k and 6.1 obtained by imagery (camera and image recovery lens) transversely to the equipment. Depending on the parameters of fluid pressure (4) at inlet (7) and suction of fluid (4) at outlet, it is possible to modulate the central thickness of the film. As an example, thicknesses of 136 μm, 100 μm and 56 μm have been obtained experimentally and are illustrated in FIGS. 6.j, 6.k and 6.1.
    The monitoring of these thicknesses correlated to the adaptation of the laser excitation parameters (energy, focusing, etc.) allows fine adjustment of the jets generated by the absorption of the laser. The upper shape of the film is not necessarily flat as can be seen in the photographs. This depends on the parameters of the fluid (4) (viscosity, density, flow, etc.) and on the pressure / suction parameters of said fluid. The laser shooting area can be spatially adapted to a part of the film where the thickness is constant. Said area can also correspond to the entire cavity, but in this case, the laser parameters will be adapted to the variations in film thickness in the target field.
    The characterization of the film could also be carried out by other means than the imagery cited here, one can for example think of means of spectroscopic analysis, of distance measurement, of shading on a line, etc.
    FIG. 7 represents a 3D version of the solution presented in FIG. 3. This embodiment, shown in 3D in FIG. 7.m, comprises 3 cavities each supplied by dedicated supply and evacuation conduits. A groove is present on the top to allow the observation of the ink films, at least those placed at the ends of the equipment. In addition, a transparency view is proposed in Figure 7.n. It allows you to see the three holes allowing the laser to pass through the part to each cavity (3) equipped with a blade (2). It is obvious that this example is purely illustrative of the wide possibilities of designs which are possible for this equipment. Indeed, one could imagine it with:
    - 2 cavities or more than 3 cavities
    - a single supply duct and / or a single exhaust duct common to all the cavities
    - a different cavity shape (square, channel, oval, diamond, etc.) of the conduits arriving parallel or perpendicular to the surface of the blades
    The examples cited here are therefore not limitative of the architectures that the equipment according to the invention could have.
    Detailed description of the printing process without sacrificial layer according to the invention
    FIG. 8 describes the main stages of the interaction between the laser and the material in the context of the printing of liquids which are homogeneous or contain inhomogeneities.
    - The first step is to focus the laser on the material, here the ink arranged in the form of a film (4) in the cavity (3). The way of focusing the laser directly impacts the volume which will absorb the deposited energy. We speak in this case of laser fluence (energy related to the surface see to the volume). As the process does not use a sacrificial layer, it is the ink and most of its liquid medium which absorbs the energy of the laser. In fact, the choice of the wavelength of the laser and of its energy has a direct impact on the absorption capacity of the film (4). In the case of bioprinting, the medium consists essentially of water which has absorption peaks well known at the spectral level. We can therefore seek to maximize this absorption by choosing laser sources corresponding to these maxima (absorption lines of water in the infrared for example). We can also seek to maximize absorption by means of absorbers placed in the ink (molecules, dyes, particles). In the examples illustrated in this present invention, the laser used works at 1030 nm of wavelength (ytterbium) for a pulse duration covering an area ranging from 10 picoseconds to 400 femtoseconds and energies between 1 and 40 pJoules. Preferably, we used the laser with a pulse duration of 10 picoseconds for an energy per pulse of 10 to 14 pJoules.
    - The second step corresponds to the creation of the plasma (81) which is the result of the dissociation of the material following the absorption of the laser by the film fluid (4). This plasma is made up of a mixture of atoms, ions, electrons, molecular residues ...
    It is created over extremely short times, typically a few picoseconds after the laser absorption, and it also has a very short “life” time of the order of a microsecond. The size of the plasma (81), its spatiotemporal dynamics, its "temperature" and its constituents are very strongly linked to the duration of the laser pulse used. If it is in a so-called “short” regime from the microsecond to the nanosecond, the main effects at the origin of the plasma are linear absorption effects with local temperature increases of the order of one to a few degrees . It is a process called thermal. It is considered to be more “rough” on the quality of plasma confinement in a well-controlled and small space. On the other hand, if the pulse duration is in a so-called “ultra-short” regime, that is to say corresponding to pulse durations of the order of a few tens of picoseconds to the femtosecond, then the effects at the origin of plasma will be a combination of linear and non-linear effects. Moreover, the shorter the pulse duration, the more the non-linear effects will be favored. The advantage of having recourse to these regimes resides in the access to so-called “athermic” processes making it possible to ensure plasma confinement in a very well bounded and very small space without temperature rise. This regime is therefore more favorable to cell viability a priori as well as to high resolution. In the case of the present invention, the main results have been obtained between 5 and 10 picoseconds, a regime which mixes both linear and non-linear effects. They made it possible to demonstrate the ability to print without sacrificial layer both homogeneous media and colloidal media.
    - The third step corresponds to the creation of the cavitation bubble (82) in the middle. This bubble is the result of the recombination of the constituents of the plasma into a gas under pressure. Recombination is based on many complex physical processes such as field effects, radiative and non-radiative recombinations, tunnel effects, etc. Cavitation is very strongly dependent on the size and quality of the initial plasma (81) . Cavitation (82) appears after approximately one microsecond following absorption by the laser and creation of the plasma. It can have a spherical shape but can also have an elongated or annular shape. It all depends on the initial plasma and its shape. The polarization of the laser and the geometric distribution of its energy at the focal plane directly influence the shape of the plasma and therefore the shape of the cavitation bubble. Thus, to obtain more reproducible results, preference will be given to isotropic forms, such as circular laser polarization.
    - Finally, the fourth step corresponds to the so-called hydrodynamic phase where the cavitation bubble (82) will grow, deform, cause movements of the liquid, etc. The various phases of these hydrodynamic phenomena are partly already known in through certain theories like those of Pearson or Wortington .... The final result is the creation of a jet of matter (83) at the level of the free surface of the liquid. The surface tension of the liquid, the distance from the bubble to the free surface, the viscosity of the liquid are among the most influential parameters on the shape and dynamics of this jet (83).
    Thus, printing without sacrificial layer will be dependent on a very large number of parameters both linked to the laser and to the ink used. The control of the ink film by the equipment described according to the invention is a means of regulating part of the possible disparities (sedimentation, drying, variable and uncontrolled thickness, etc.) during printing. In addition, the possibilities of modulating the flow rate and the thickness of said film by the pressure and suction means could make it possible to modulate the size, the shape and the dynamics of the jets. Thus, with such an invention, it becomes possible to reduce the range of laser parameters necessary to modulate the jets. The direct impact of such a choice would be to use a laser that is much simpler in its definition, more stable and above all much less expensive since it is less versatile.
    Figure 9 illustrates real material jets, generated by laser without sacrificial layer. The 4 photographs in this figure each correspond to a specific time after focusing the laser shot in the ink film (4). The first photo was taken 5ps after the shot, the second 50ps after and so on. This shadow imaging technique is commonly called time resolved imaging. It allows you to decompose hyper-rapid events by taking photos thanks to very short lighting times. This series of photos makes it possible to illustrate the principle of the generation of jets by laser as explained in the preceding figure 8. We can see in short time the creation of a pyramid-shaped dome surmounted by a very fine first jet then in long time we can see the rise of a much more imposing jet from which one or more drops are detached. Depending on the distance between the free surface of the film (4) and the receiving substrate on which we want to print, it is one or more of these droplets which will settle. Sometimes, it can happen that the distance between the ink and the printing substrate is small enough, in general less than 500 μm, for the jet to directly intercept the surface of the receiving substrate. This is called a transfer regime. In all cases, whether the mechanism is droplet deposition or transfer, we will speak of laser printing towards the front.
    The following figures 10, 11, 12, 13 illustrate the results obtained in sacrificial printing. These results prove that the j parameters used in the context of this invention:
    and 14 layer them with laser (picosecond regime, ten microJoules, wavelength in the near infrared, polarization ...)
    - ink (viscosity, surface tension, density, thickness ...) system (scanning speed, pattern used, focusing ...) allow to print homogeneous and inhomogeneous objects, which had never been demonstrated before .
    Thus, FIG. 10 presents a very reproducible result of laser printing without the sacrificial layer of a homogeneous ink mainly consisting of water. Each drop printed appears as a small circle on the image. The large circle (separating the gray area from the black area) simply corresponds to the field imaged by the microscope used to take this picture. The printed drops are typically 100 μm in diameter and are 500 μm apart from one another. This result was obtained in the equipment according to the invention in static mode (without continuous recharging).
    FIG. 11 shows a homogeneous result of laser printing without sacrificial layer of a colloidal ink made of water, surfactant and microbeads of 5 μm in diameter each. The result of the printing shows the capacity of this invention to deposit droplets enclosing a small number of microbeads, on average 2 to 3 per droplet. This is proof that printing without a sacrificial layer can achieve very high resolution performance on colloidal media (which had never been demonstrated before). This result was obtained in the equipment according to the invention in static mode (without continuous recharging).
    FIG. 12 shows a relatively homogeneous result of laser printing without a sacrificial layer of cellular ink. This impression constitutes a first as for microbeads. It is very convincing and opens up very wide fields of use for this invention. The disparities in printing visible on the image are essentially linked to the disparities in the ink used and deposited on the slide (3) which has sedimented and aggregated in bundles. Indeed, this result was obtained in static mode, that is to say without operating the equipment according to the invention in a dynamic continuous recharging mode. The aim of this result was above all to prove the ability of gold-free printing to print living cells. Again, this result was obtained in the equipment according to the invention in static mode (without continuous recharging).
    FIG. 13 presents a relatively homogeneous printing result of laser printing without sacrificial layer of a homogeneous ink obtained with the equipment according to the invention working in dynamic mode, that is to say with the fluidic system working in continuous reloading .
    Finally, Figure 14 is another illustration of the ability of sacrificial layerless printing technology to be optimized as needed. Indeed, on this image, we can see a field of droplets printed under the same conditions as those described so far with only one difference: the presence of an absorbent agent incorporated in the ink. In this example, it was melanin, a natural biological compound with very high absorption at the wavelength of the laser used for these experiments, namely 1030nm. Thus, the addition of this compound made it possible to work at lower laser energies to allow the laser absorption, then the creation of the plasma and finally the hydrodynamics of the cavitation bubble. This result was obtained in the equipment according to the invention in static mode (without continuous recharging).
    权利要求:
    Claims (20)
    [1" id="c-fr-0001]
    Claims
    1 - Additive printing equipment comprising an orientable energy excitation means for producing a punctual interaction with a fluid covering a blade (2), in order to cause a jet oriented towards a target (11) (11), said fluid consisting of a liquid vector containing transferable inhomogeneities, characterized in that said fluid forms a liquid film (4) with a thickness less than 500 μm, on a blade (2) having at least one zone (3) allowing the interaction with the laser into which opens at least one inlet (7), said interaction zone (3) opening into at least one outlet (9)
    The equipment further comprising means for circulating the fluid between said inlet (7) and said outlet (9).
    [2" id="c-fr-0002]
    2 - Additive printing equipment according to claim 1 characterized in that the thickness of said film (4) is between 20 and 100 pm.
    [3" id="c-fr-0003]
    3 - Additive printing equipment according to claim 1 characterized in that the thickness of said film (4) is between 3 and 10 times the nominal size of said transferable particles.
    [4" id="c-fr-0004]
    4 - Additive printing equipment according to claim 1 characterized in that the surface of said interaction zone (3) is greater than 0.05 mm 2 . 5
    [5" id="c-fr-0005]
    5 - Additive printing equipment according to claim 1 characterized in that said inlet (7) opens into a lateral part of said interaction zone (3).
    [6" id="c-fr-0006]
    6 - Additive printing equipment according to claim 1 characterized in that said interaction zone (3) has a peripheral part opening laterally into said outlet (9).
    [7" id="c-fr-0007]
    7 - Additive printing equipment according to claim 1 characterized in that said inlet (7) and said outlet (9) are constituted by tubular channels connected to the connection zone (3), the longitudinal axes of each of said tubular channels (7, 9) forming with the transverse plane of the interaction zone between 15 ° and 35 °.
    [8" id="c-fr-0008]
    8 - Additive printing equipment according to claim 1 characterized in that said means for circulating the fluid between said inlet (7) and said outlet (9) comprise means for controlling the injection flow rate (or the positive pressure exerted on the fluid) and the suction flow to control the flow of the fluid (or the negative pressure exerted on the fluid) in the interaction zone.
    [9" id="c-fr-0009]
    9 - Additive printing equipment according to the preceding claim characterized in that said means for controlling the injection rate and the suction rate are controlled by measuring the thickness of the film (4), to control the thickness of the film (4) in the interaction zone (3).
    [10" id="c-fr-0010]
    10 - Additive printing equipment according to claim 1 characterized in that it comprises a plurality of interaction zones each comprising an inlet (7) and an outlet (9).
    [11" id="c-fr-0011]
    11 - Additive printing equipment according to the preceding claim characterized in that at least two of said interaction zones have common inputs (7) and / or outputs (9).
    [12" id="c-fr-0012]
    12 - Additive printing equipment according to claim 1 characterized in that said energetic excitation means is constituted by a laser.
    [13" id="c-fr-0013]
    13 - Additive printing equipment according to the preceding claim characterized in that the interaction zone is transparent in the wavelength band of the laser and of the imaging and does not have a sacrificial layer.
    [14" id="c-fr-0014]
    14 - Additive printing equipment according to the preceding claim characterized in that said fluid is charged with an absorbent pigment in the emission wavelength of the laser.
    [15" id="c-fr-0015]
    15 - Additive printing equipment according to claim 12 characterized in that it comprises means for imaging the interaction zone for controlling the laser as a function of the density of inhomogeneities.
    [16" id="c-fr-0016]
    16 - Additive printing equipment according to claim 12 characterized in that said laser emits pulses in picosecond or femtosecond regime with an energy level between 20 and 40 microjoules, the energy level per pulse being controlled by a computer depending on the measurement result of the characteristics of the fluid present in the interaction zone, said measurements comprising the density in non-uniformities, and / or the viscosity, and / or the thickness of the film (4).
    [17" id="c-fr-0017]
    17 - Additive printing equipment according to claim 1 characterized in that said energetic excitation means is constituted by an acoustic wave generator.
    [18" id="c-fr-0018]
    18 - Additive printing equipment according to claim 1 characterized in that it comprises means for imaging the interaction zone and for selecting the type of particle to be transferred.
    [19" id="c-fr-0019]
    19 - Method of additive printing by equipment comprising a steerable energizing excitation step to produce a punctual interaction with a fluid covering a blade (2), in order to cause a jet oriented towards a target (11), said fluid consisting of a liquid vector containing transferable inhomogeneities, characterized in that said fluid forms a liquid film (4) with a thickness less than 500 μια flowing between an inlet conduit (7) and an outlet conduit (9 ) a blade (2) having at least one zone allowing interaction with the laser, and into which opens at least one entry (7).
    [20" id="c-fr-0020]
    20 - Additive printing method according to the preceding claim characterized in that the energy level per pulse is controlled by a computer as a function of the measurement result of characteristics of the fluid present in the interaction zone, said measurements comprising the density in particles, and / or viscosity, and / or the thickness of the film (4).
    1/12 Fig. 1
    2/12
    3/12
    类似技术:
    公开号 | 公开日 | 专利标题
    EP3595592B1|2021-11-03|Equipment and method for additive manufacturing
    EP3234102B1|2018-06-27|Method for laser printing biological components, and device for implementing said method
    EP3233499B1|2019-05-22|Laser printing method, and device for implementing said method
    CA2574332C|2012-10-02|Microfluidic circuit with an active component
    WO2013153195A1|2013-10-17|Laser nanomachining device and method
    Duocastella et al.2015|Sub-wavelength laser nanopatterning using droplet lenses
    FR3010918B1|2019-07-26|DEVICE FOR APPLYING PROJECTED COATINGS ON PARTS AND ASSOCIATED METHOD
    EP2367614B1|2013-12-11|Device for depositing a powder mixture for forming an object with composition gradients, and related method
    Breckenfeld et al.2016|Laser-induced forward transfer of Ag nanopaste
    EP3393820B1|2021-09-22|Process for producing, by etching through a lenticular grating, images that may be selectively viewed by varying angle of observation
    FR3093944A1|2020-09-25|CARTRIDGE FOR BIOIMPRESSION
    WO2020109745A1|2020-06-04|Additive manufacturing method assisted by a granular constrained medium
    Chan et al.2012|Surface nanobubble nucleation visualized with TIRF Microscopy
    FR3110107A1|2021-11-19|Additive manufacturing process in an adjustable constrained environment
    FR2899147A1|2007-10-05|Decorative laser marking on non-flat parts by using decorative materials e.g. plastics, comprises modeling and generating of three-dimensional polylines in space, generating three-dimensional vectors, and real-time processing of vectors
    BE1025341B1|2019-02-04|METHOD FOR STRUCTURING A SUBSTRATE
    EP3595904B1|2021-05-05|Apparatus and method for depositing particles on a target
    FR3102377A1|2021-04-30|Equipment and process for deposition by projection of particles by laser shock waves
    EP3595901B1|2021-05-05|Apparatus for the transfer of bio-ink
    Serra et al.2013|Film-free laser microprinting of transparent solutions
    FR2993789B1|2019-06-28|METHOD AND INSTALLATION OF NANO-POROUS MEMBRANE FORMATION
    Lobos Caparrós2015|Manufactured microlenses by polimer inkjet printing
    同族专利:
    公开号 | 公开日
    KR20190126884A|2019-11-12|
    EP3595592B1|2021-11-03|
    JP2020513850A|2020-05-21|
    FR3063931B1|2019-03-22|
    CN110402125A|2019-11-01|
    EP3595592A1|2020-01-22|
    US20200102529A1|2020-04-02|
    WO2018167402A1|2018-09-20|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    WO2016097620A1|2014-12-17|2016-06-23|Universite de Bordeaux|Method for laser printing biological components, and device for implementing said method|
    WO2016097619A1|2014-12-17|2016-06-23|Universite de Bordeaux|Laser printing method, and device for implementing said method|
    WO2017011854A1|2015-07-22|2017-01-26|Inventia Life Science Pty Ltd|Process for printing 3d tissue culture models|
    US6561643B1|1997-06-30|2003-05-13|Hewlett-Packard Co.|Advanced media determination system for inkjet printing|
    JP2012518188A|2009-02-18|2012-08-09|ソニー株式会社|Printing bioreactive materials|
    KR20100107634A|2009-03-26|2010-10-06|삼성전자주식회사|Manufacturing method for biochip|
    CN102765257B|2012-08-10|2015-02-25|李支斌|Constant-pressure buffer distributor of printer ink pipeline|
    WO2014061024A1|2012-10-21|2014-04-24|Photon Jet Ltd|A multi-technology printing system|
    CN205818681U|2016-02-26|2016-12-21|多佛欧洲有限责任公司|The dismantled and assembled assembly of single-piece for the ink loop of continuous inkjet printers|
    CN106046940A|2016-07-19|2016-10-26|青岛良友科技发展有限公司|Environment-friendly inkjet printing ink composition for common printing media and raw wallpaper, as well as preparation method and application thereof|FR3093944B1|2019-03-22|2021-03-19|Poietis|CARTRIDGE FOR BIOIMPRESSION|
    DE102019115780B4|2019-06-11|2021-10-21|Technische Universität Dresden|Methods and devices for multiphoton printing and for the inspection of three-dimensional structures|
    CN113085185A|2021-04-08|2021-07-09|北京理工大学|Method for inducing forward transfer of liquid film based on time domain shaping femtosecond laser|
    法律状态:
    2018-01-18| PLFP| Fee payment|Year of fee payment: 2 |
    2018-09-21| PLSC| Publication of the preliminary search report|Effective date: 20180921 |
    2020-03-19| PLFP| Fee payment|Year of fee payment: 4 |
    2021-03-18| PLFP| Fee payment|Year of fee payment: 5 |
    优先权:
    申请号 | 申请日 | 专利标题
    FR1752131|2017-03-15|
    FR1752131A|FR3063931B1|2017-03-15|2017-03-15|EQUIPMENT AND METHOD FOR ADDITIVE PRINTING|FR1752131A| FR3063931B1|2017-03-15|2017-03-15|EQUIPMENT AND METHOD FOR ADDITIVE PRINTING|
    EP18712977.0A| EP3595592B1|2017-03-15|2018-03-08|Equipment and method for additive manufacturing|
    PCT/FR2018/050536| WO2018167402A1|2017-03-15|2018-03-08|Equipment and method for additive manufacturing|
    JP2019571801A| JP2020513850A|2017-03-15|2018-03-08|Additional printing devices and methods|
    CN201880017567.6A| CN110402125A|2017-03-15|2018-03-08|Device and method for increasing material manufacturing|
    US16/494,737| US20200102529A1|2017-03-15|2018-03-08|Equipment and method for additive manufacturing|
    KR1020197030353A| KR20190126884A|2017-03-15|2018-03-08|Stacked Printing Equipment and Methods|
    [返回顶部]