• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    Diffractive device (18) for chemical and biological analysis based on receivers structured on waveguides, comprising a waveguide (1) and a recognition element (3) consisting of a network of receivers (11) arranged on the guide wave (1), intended to interact with target compounds (12) present in a sample, a radiation source (4), which emits an incident beam (5), which propagates through the waveguide (1) interacting with the recognition element (3) and diffracting according to the Bragg laws, generating a reflected beam (6) and a transmitted beam (7) that are collected by optical analyzers (8 and 17), which record parameters of the reflected beam (6) and transmitted beam (7), achieving multiple analyzes in a simple, fast, sensitive, quantitative way, without marking and in real time. (Machine-translation by Google Translate, not legally binding)
    公开号:ES2749689A1
    申请号:ES201930661
    申请日:2019-07-17
    公开日:2020-03-23
    发明作者:Oliver Jose Miguel Avella；Catala Angel Maquieira；Dolz Augusto Miguel Juste；Sanchez Maria Estrella Fernandez；Abellan Daniel Pastor；Munoz Pascual Munoz；Pinar Martina Delgado；Bou Miguel Vicente Andres
    申请人:Universidad Politecnica de Valencia；Universitat de Valencia；
    IPC主号:
    专利说明:

    [0001]
    [0002]
    [0003]
    [0004] OBJECT OF THE INVENTION
    [0005]
    [0006] The object of the present invention is a chemical and biological analysis diffractive device based on structured receivers on waveguides.
    [0007]
    [0008] BACKGROUND OF THE INVENTION
    [0009]
    [0010] Chemical and biological analysis techniques play a fundamental role in many key areas in current living standards, such as clinical diagnosis, environmental analysis, food safety, quality control in industry, and scientific innovation, among many others.
    [0011]
    [0012] Society currently demands cheap, compact, simple, sensitive and robust systems that allow chemical and biological analyzes to be carried out in situ, outside laboratories and hospitals, and by non-specialized users. Some prominent examples of these test systems are glucose meters and pregnancy tests.
    [0013]
    [0014] Within the particular framework of the present invention, there are currently three lines related to the development of devices for chemical and biological analysis that should be mentioned. First, biogratings ( Anal. Chem. 2017, 89, 9002-9008; Anal. Chim. Acta 2018, 1033, 173-719; Sensors 2018, 18, 3163), which are diffractive networks of bioreceptors arranged on solid surfaces unstructured.
    [0015]
    [0016] These networks are interrogated using diffraction-based sensing, which is based on irradiating the biogratings in free space (unguided radiation) and recording the intensity of the diffracted orders in free space to quantify the magnitude of the biointeraction.
    [0017]
    [0018] The second is the one described in US 2016/0139115 A1, and which consists of creating biogratings arranged according to a Fresnel lens structure on a flat waveguide, so that they interact with the guided radiation to diffract in space free according to a diffracted pattern defined by a point, the intensity of which is linked to the biointeraction of interest.
    [0019]
    [0020] On the other hand, within this second line, document US 2019/0017938 A1 describes the same principle, with the difference that biograting couples the incident beam to the plane waveguide, instead of decoupling it into free space.
    [0021]
    [0022] The third line is the fiber Bragg gratings (FBGs) and long-period fiber gratings (LPGs) ( Anal. Bioanal. Chem. 2015, 407, 3883-3897; Nanophotonics 2017, 6, 663-679; Anal. Chem. 2016, 88, 203-227). In both, optical fibers are irradiated to record periodic refractive index variations capable of interacting with the incident beam according to Bragg's laws.
    [0023]
    [0024] In its bioanalytical application, unstructured receptor layers are placed on the FBGs and LPGs, commonly on a gold layer introduced to generate plasmonic phenomena.
    [0025]
    [0026] In any case, there are a number of aspects that these three lines cannot resolve. First, there is a need to develop label-free systems for quantitative analysis in non-specialized environments. Secondly, most of these technologies are not capable of discriminating the signal contributions generated by nonspecific interactions, so there is no possibility of analyzing complex real samples selectively and minimizing the steps of sample preparation.
    [0027]
    [0028] DESCRIPTION OF THE INVENTION
    [0029]
    [0030] The chemical and biological analysis diffractive device object of the present invention provides solutions to these aspects still to be solved in the field of chemical and biological analyzers, such as the development of label-free systems for quantitative analysis in non-specialized environments. .
    [0031]
    [0032] This device also allows biomolecular interactions to be measured in real time ( realtime), to record a multitude of analyzes in a single measurement ( multiplexing), and has great potential to design compact , portable ( point -to-chip) miniaturized analyzers ( point -of-care).
    [0033] It also solves non-specific adsorption problems in the analysis of complex samples (blood, serum, saliva, etc.). Unlike most state-of-the-art technologies, it can discriminate signal contributions generated by nonspecific interactions. This allows complex real samples to be analyzed selectively and minimizing the sample preparation steps.
    [0034]
    [0035] In addition, it is a miniaturizable device and integrable in telecommunications systems, and it is simple, compatible with cheap and technologically available materials and devices, which gives it the ability to be implemented in handheld devices.
    [0036]
    [0037] Specifically, the device object of the present invention comprises a waveguide, preferably a flat waveguide integrated in a substrate or an optical fiber, with an inlet at one end, an outlet at the opposite end, and a test zone in its central part, on which an element of recognition is positioned.
    [0038]
    [0039] The chemical composition of the waveguide can be glass, doped glass, silicon, doped silicon, or polymers, such as polymethylmethacrylate or polystyrene, among other materials.
    [0040]
    [0041] The recognition element comprises a network of receivers that are arranged over the test area. The receptor network is a set of elements, such as organic molecules, biomacromolecules, microorganisms or synthetic biomimetic compounds, structured according to a diffraction grating that meets the Bragg conditions. The receptor network is intended to selectively interact with target compounds, present in a sample to be analyzed.
    [0042]
    [0043] Specifically, the receptor network could be a set of antibodies, enzymes, proteins, nucleic acids, molecularly imprinted polymers, polysaccharides, protein-hapten complexes, organic molecules, bacteria, viruses or tissues.
    [0044]
    [0045] Target compounds with which the receptor network interact can be metabolites, drugs, drugs, contaminants, biomarkers, pathogens, chemical weapons, biological weapons or allergenic agents.
    [0046]
    [0047] The network of receivers is distributed over the test area of the waveguide, forming immobilized receptor zones that alternate with holes without receivers. This Structure of immobilized receptor areas and gaps is periodically distributed throughout the test area. The morphology and dimensions of this structure are such that it meets the Bragg conditions.
    [0048]
    [0049] The analytical sensitivity of this device increases with various parameters and, in particular, with the length of the test area over which the network of receivers is distributed, a property that allows the sensitivity of the device to be adapted to that required for each application.
    [0050] The receiver network can be manufactured using techniques such as micro contact printing, photolithography, laser beam interference, or holographic techniques.
    [0051]
    [0052] The immobilization of the receptor network on the test zone can be done by fissionation or covalent anchoring processes such as thiol-ene coupling or the carbodiimide reaction.
    [0053]
    [0054] Said immobilization may require a previous activation / functionalization stage of the test area, such as ultraviolet irradiation, organosilane treatment or ozone treatment. Likewise, immobilization of the receptor network can be done by using compounds such as hydrogels, proteins A and G, avidin or strepvidin or biotinylated products.
    [0055]
    [0056] The recognition element may additionally comprise blocking agents that are positioned in the gaps without receptors. Blocking agents improve the analytical performance of the device. Blocking agents can be, for example, proteins, surfactants or glycols.
    [0057]
    [0058] On the other hand, the device also comprises a source of electromagnetic radiation, which emits an incident beam towards its entrance, the incident beam propagating through the waveguide.
    [0059]
    [0060] The incident beam interacts with the recognition element, which diffracts it according to the Bragg conditions, generating a reflected beam and a transmitted beam. The reflected beam exits through the input of the waveguide and the beam transmitted through the output of the waveguide.
    [0061]
    [0062] The radiation source generates electromagnetic radiation, preferably in the ultraviolet, visible or infrared spectrum, which can be monochromatic or polychromatic. Some Examples of radiation sources could be lasers, super-continuous sources, LED diodes, incandescent lamps, and halogen lamps.
    [0063]
    [0064] To analyze both beams, the device comprises at least one optical analyzer capable of registering the parameters of the electromagnetic radiation beams.
    [0065]
    [0066] The optical analyzer is positioned between the radiation source and the waveguide input and is intended to record the optical parameters of the reflected beam. The analyzer can also be positioned at the output of the waveguide and intended to record the optical parameters of the transmitted beam.
    [0067]
    [0068] Optical analyzers can be spectrophotometers, optical spectrum analyzers, photodiodes, or CMOS cameras.
    [0069]
    [0070] Some of the optical parameters of interest to be measured by optical analyzers are wavelength, frequency, amplitude, intensity or phase of the beams.
    [0071]
    [0072] In order to analyze a sample of interest, it must come into contact with the test area. The target compounds present in the sample interact with the receptor network, this interaction modifying the amount of matter present in the receptor areas with respect to the gaps, which affects the optical magnitudes of the reflected beam and / or the transmitted beam.
    [0073]
    [0074] In this way, through the measurement of the optical parameters of the reflected beam and / or of the transmitted beam, the chemical and biological parameters of analytical interest related to the receptors, the target compounds and / or the interaction between both of them.
    [0075]
    [0076] Some chemical and biological parameters of the sample that can be analyzed using the present invention are the concentration of the target compound in the sample, the activity of the target compound, the surface density of the receptors, the activity of the receptors, and the constants. kinetics and thermodynamics involved in the interaction between receptors and target compounds.
    [0077] Additionally, the device may comprise a first optical device located between the radiation source and the input of the waveguide and a second optical device, positioned at the output of the waveguide.
    [0078] Both optical devices modify the optical properties of the radiation beams to adapt their coupling and guidance through the waveguide, their interaction with the recognition element and / or subsequent registration in the optical analyzers. Optical devices may also be intended to discriminate beams that are propagated by the waveguide.
    [0079]
    [0080] The first optical device and the second optical device may be polarization controllers, polarizers, attenuators, monochromators, circulators, couplers, Bragg networks, or long-period networks, as well as integrating more than one of these elements.
    [0081]
    [0082] The waveguide can have different morphologies in parts or in its entirety to adapt the spread of the beams and their measurement, and can be made up of one or multiple layers.
    [0083]
    [0084] The device may additionally comprise coatings that are placed on the waveguide. The coatings can comprise one or more layers that confer mechanical properties to the waveguide and improve the optical response by adapting the propagation of the radiation beams through it.
    [0085]
    [0086] Part of the radiation emitted by the radiation source is propagated by the waveguide, as well as by its surface, interacting with the recognition element. To facilitate this interaction, both the waveguide and the coating may have modifications, such as alterations in the morphology and composition of the waveguide and the coating.
    [0087]
    [0088] The device object of the invention can be positioned on a support that supports all the elements that make it up and that can additionally comprise fluidic systems (pumps, channels, chambers, etc.) to automate the handling of samples and solutions.
    [0089]
    [0090] The device allows multiplexing by incorporating several recognition elements located in series and structured each with diffraction gratings of different periods in the test area. In this way, each recognition element will produce a reflection and transmission signal located in different areas of the optical spectrum, allowing its unique identification.
    [0091] DESCRIPTION OF THE DRAWINGS
    [0092]
    [0093] To complement the description that is being made and in order to help a better understanding of the characteristics of the invention, according to a preferred example of practical embodiment thereof, a set of drawings is included as an integral part of said description. where, by way of illustration and not limitation, the following has been represented:
    [0094]
    [0095] Figure 1.- Shows a general diagram of a first embodiment of the diffractive device for chemical and biological analysis.
    [0096]
    [0097] Figure 2.- Shows a detailed diagram of the network of receivers of the recognition element.
    [0098]
    [0099] Figure 3.- Shows a detailed diagram of the recognition element when it also includes blocking agents.
    [0100]
    [0101] Figure 4.- Shows a general diagram of a second embodiment of the diffractive device for chemical and biological analysis.
    [0102]
    [0103] PREFERRED EMBODIMENT OF THE INVENTION
    [0104]
    [0105] An example of embodiment of the diffractive device for chemical and biological analysis is described below with the help of Figures 1 to 4.
    [0106]
    [0107] The device (18) object of the present invention, shown in figure 1 in a first embodiment, comprises a waveguide (1) which is a flat waveguide (1) integrated in a substrate, and which comprises a inlet (19) at one end, an outlet (20) at the opposite end and a test area (2). A recognition element (3) is positioned on the test area (2).
    [0108]
    [0109] In a second embodiment of the invention, which appears in figure 4, the waveguide (1) is a cylindrical fiber and the recognition element (3) is positioned by wrapping it in the part that encompasses the test area (2), the waveguide (1) and the recognition element (3) being concentric.
    [0110] Specifically, in this second embodiment, the waveguide (1) is a single-mode optical fiber 125 microns in diameter. The test zone (2) is generated by narrowing the waveguide (1) to a diameter of between 1 and 5 microns. Narrowing is done mechanically by combining stretching with heating with a flame.
    [0111]
    [0112] For its part, the recognition element (3) comprises a network of receivers (11) that are arranged over the test area (2) of the waveguide (1). The network of receivers (11) is manufactured by means of micro contact stamping and is covalently immobilized.
    [0113]
    [0114] The network of receivers (11) is distributed over the test zone (2) forming immobilized receptor zones (13) that alternate with holes (14) without receivers. This structure of receptor zones (13) and voids (14) is periodically distributed throughout the test zone (2). The receptors (11) that make up the receptor areas (13) are proteins.
    [0115]
    [0116] The recognition element (3) also comprises blocking agents (15) that are positioned in the gaps (14) without receptors. Blocking agents (15) are polysorbate molecules.
    [0117]
    [0118] The receptor zones (13) and the recesses (14) have the same dimensions, they extend rectilinearly in the transverse direction to the test zone (2) with a periodicity of around 550 nanometers.
    [0119]
    [0120] Furthermore, the device (18) comprises a radiation source (4), connected to the input (19) of the waveguide (1), which emits an incident beam (5) in the direction of the input (19), and that propagates through the waveguide (1).
    [0121] The radiation source (4) is a 1.3 mW SuperLed lamp with a maximum emission of 1550 nm, which is connected to the waveguide (1).
    [0122]
    [0123] The incident beam (5) interacts with the recognition element (3) generating a reflected beam (6) and a transmitted beam (7). The reflected beam (6) leaves through the input (19) of the waveguide (1) and the transmitted beam (7) through the output (20) of the waveguide (1).
    [0124] To carry out an analysis of a liquid sample that contains antibodies that constitute target compounds (12), said sample is incubated on the test area (2), and after incubation its concentration is quantified using the information collected from the beam. reflected (6) or the transmitted beam (7). Specifically, the maximum peak intensity of the reflected beam (6) and the minimum valley intensity of the transmitted beam (7) are determined.
    [0125]
    [0126] Additionally, the device (18) comprises a first optical device (9) located between the radiation source (4) and the input (19) of the waveguide (1) and a second optical device (16), positioned close to the output (20) of the waveguide (1). Both optical devices (9, 16) comprise a polarizer, a polarization controller and a circulator.
    [0127]
    [0128] The device (18) also comprises a first optical analyzer (8) and a second optical analyzer (17), capable of recording the parameters of the electromagnetic radiation beams.
    [0129]
    [0130] The first analyzer (8) is positioned between the radiation source (4) and the input (19) of the waveguide (1) and is intended to record the optical parameters of the reflected beam (6). The second analyzer (17) is positioned close to the output (20) of the waveguide (1) and is intended to record the optical parameters of the transmitted beam (7).
    [0131]
    [0132] The optical analyzers (8 and 17) are optical spectrum analyzers for the infrared interval, connected to the waveguide (1) through the optical devices (9 and 16).
    [0133]
    [0134] In a third embodiment of the invention, a portion of the test zone (2) is laterally eliminated to generate a flat surface ( D-shaped fibers), being able to eliminate part of the waveguide (1). This type of test zones (2) can be obtained by mechanical polishing or by chemical dissolution.
    [0135]
    [0136] Furthermore, the waveguide (1) and / or the optical devices (9 and 16) comprise additional elements such as Bragg networks or long-period networks, to modify the optical properties of the radiation beams (5, 6 and 7). and / or its interaction with the network of receivers (11), so as to allow, for example, obtaining different optical responses.
    [0137]
    [0138] The device (18) may also comprise different recognition elements (3), each with its optical response tuned to a different wavelength, modifying the period of the receiver network (11) and / or its inclination with respect to the guide of wave (1), or the dimensions of the test area (2), so that they present different and non-overlapping spectral responses in the reflected (6) and / or transmitted (7) beam, thus allowing multiple different analyzes to be carried out in one only sample.
    [0139]
    [0140] When necessary, multiple test zones (2) can be connected in series or in parallel, each with at least one recognition element (3), all of them being tuned to different wavelengths modifying the period of the receiver network (11) and / or its inclination with respect to the test zone (2) and / or the geometry or composition of the waveguide (1) along the test zone (2).
    [0141]
    [0142] In this way, each test zone (2) presents a different spectral response in the reflected beam (6) and / or the transmitted beam (7), thus allowing multiple analyzes to be carried out both on a single sample and on multiple samples.
    [0143]
    [0144] Using the device (18), the information of the beams can be recorded during the incubation of the sample, so that real-time information is obtained on the biorecognition events of the target compound (12), from which determines the concentration of the target compound (12) and / or the affinity constants between the receptors (11) and the target compound (12).
    权利要求:
    Claims (12)
    [1]
    1. - Chemical and biological analysis diffractive device (18), intended to analyze a sample comprising target compounds (12), characterized in that it comprises:
    - a waveguide (1) with an inlet (19) at one end, an outlet (20) at the opposite end and a test area (2), on which it is positioned
    - a recognition element (3) comprising a network of receivers (11) that is distributed over the test area (2) forming areas with receivers (13), which alternate with gaps (14) without receivers, being the recognition element (3) intended to interact with the target compounds (12),
    - a radiation source (4), which emits an incident beam (5) towards the input (19) of the waveguide (1), which propagates through it and is diffracted by the recognition element (3) fulfilling the Bragg conditions, generating a reflected beam (6) that leaves through the entrance (19) and a transmitted beam (7) that leaves through the exit (20),
    - at least one optical analyzer (8, 17), which records optical parameters of the reflected beam (6) and / or of the transmitted beam (7).
    [2]
    2. - The device (18) of claim 1, further comprising a first optical device (9) located between the radiation source (4) and the input (19) of the waveguide (1), intended to modify radiation beams (5, 6, 7).
    [3]
    3. - The device (18) of claim 1, further comprising a second optical device (16), positioned at the output (20) of the waveguide (1), intended to modify the radiation beams (5, 6 and 7).
    [4]
    4. - The device (18) of claim 1 wherein the radiation source (4) is a device selected from a laser, an LED diode, an incandescent lamp and a halogen lamp.
    [5]
    5. - The device (18) of claim 2, wherein the first optical device (9) is a device selected from a polarization controller, a polarizer, an attenuator, a monochromator, a circulator, a coupler, a network Bragg and a long period network.
    [6]
    6. - The device (18) of claim 3, wherein the second optical device (16) is a device selected from a polarization controller, a polarizer, an attenuator, a monochromator, a circulator, a coupler, a network Bragg and a long period network.
    [7]
    7. - The device (18) of claim 1, wherein the waveguide (1) is a waveguide selected from an optical fiber, a narrowed optical fiber, a D-shaped type optical fiber and an optical guide integrated.
    [8]
    8. - The device (18) of claim 1, wherein the waveguide (1) is of a material selected from glass, doped glass, silicon, doped silicon, polymers, polymethylmethacrylate and polystyrene.
    [9]
    9. - The device (18) of claim 1, further comprising coatings (10) positioned on the waveguide (1) that confer mechanical and optical properties to the waveguide (1).
    [10]
    10. - The device (18) of claim 1, wherein the receptor network (11) is a set of elements selected from antibodies, enzymes, proteins, nucleic acids, molecularly imprinted polymers, polysaccharides, protein-hapten complexes , bacteria, viruses and tissues.
    [11]
    11. - The device of claim 1, wherein the recognition element (3) further comprises blocking agents (15) that are positioned in the gaps (14) without receptors.
    [12]
    12. - The device (18) of claim 1, wherein the first optical analyzer (8) and the second optical analyzer (17) are selected from a spectrophotometer, an optical spectrum analyzer, a photodiode and a CMOS camera.
    类似技术:
    公开号 | 公开日 | 专利标题
    Brecht et al.1997|Recent developments in optical transducers for chemical or biochemical applications
    US6239876B1|2001-05-29|Optical detector device
    Washburn et al.2010|Quantitative, label-free detection of five protein biomarkers using multiplexed arrays of silicon photonic microring resonators
    US7309614B1|2007-12-18|Self-referencing biodetection method and patterned bioassays
    US7101660B2|2006-09-05|Method for producing a colorimetric resonant reflection biosensor on rigid surfaces
    ES2336466T3|2010-04-13|OPTICAL SENSORS OF MULTIPLE WAVE LENGTHS.
    AU2008242664B2|2011-10-20|Method for employing a biosensor to detect small molecules that bind directly to immobilized targets
    WO2010030251A2|2010-03-18|Integrated optical sensors operating in the frequency domain
    Barrios2012|Integrated microring resonator sensor arrays for labs-on-chips
    Gauglitz2020|Critical assessment of relevant methods in the field of biosensors with direct optical detection based on fibers and waveguides using plasmonic, resonance, and interference effects
    EP2689233A1|2014-01-29|Optical sensing device for sensing analytes and related apparatus and methods
    Katiyi et al.2018|Si nanostrip optical waveguide for on-chip broadband molecular overtone spectroscopy in near-infrared
    JP2008527395A|2008-07-24|Method for surface plasmon spectroscopy in a surface plasmon resonance sensor and element for its use
    Psarouli et al.2015|Monolithically integrated broad-band Mach-Zehnder interferometers for highly sensitive label-free detection of biomolecules through dual polarization optics
    Joseph et al.2020|Grating-Coupled Surface Plasmon-Polariton Sensing at a Flat Metal–Analyte Interface in a Hybrid-Configuration
    Berneschi et al.2020|Optical whispering gallery mode resonators for label-free detection of water contaminants
    Duval et al.2015|Optical waveguide biosensors
    ES2636713B2|2018-05-18|PHOTONIC SENSOR DEVICE, SAMPLE ANALYSIS METHOD THAT MAKES SAME USE AND USES OF SUCH DEVICE
    WO2013053930A1|2013-04-18|Resonant biosensor
    Barrios et al.2012|Molecularly imprinted polymer for label-free integrated optical waveguide bio | sensors
    ES2749689B2|2020-12-16|DIFRACTIVE DEVICE FOR CHEMICAL AND BIOLOGICAL ANALYSIS
    Cennamo et al.2021|Biosensors exploiting unconventional platforms: The case of plasmonic light-diffusing fibers
    Yang et al.2021|Gradient waveguide thickness guided-mode resonance biosensor
    Sumriddetchkajorn et al.2010|A highly sensitive optofluidics-based refractometer in a Young interferometer design
    Shi et al.2020|Amplitude Holographic Interference-Based Microfluidic Colorimetry at the Micrometer Scale
    同族专利:
    公开号 | 公开日
    CA3144564A1|2021-01-21|
    WO2021009397A1|2021-01-21|
    ES2749689B2|2020-12-16|
    AU2020312756A1|2022-02-03|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    US5864641A|1997-04-11|1999-01-26|F&S, Inc.|Optical fiber long period sensor having a reactive coating|
    WO1999054714A1|1998-04-20|1999-10-28|Universiteit Twente|Integrated optical lightguide device|
    EP2827130A1|2013-07-15|2015-01-21|F. Hoffmann-La Roche AG|Device for use in the detection of binding affinities|
    DE102017211910A1|2017-07-12|2019-01-17|Dr. Johannes Heidenhain Gmbh|Diffractive biosensor|
    法律状态:
    2020-03-23| BA2A| Patent application published|Ref document number: 2749689 Country of ref document: ES Kind code of ref document: A1 Effective date: 20200323 |
    2020-12-16| FG2A| Definitive protection|Ref document number: 2749689 Country of ref document: ES Kind code of ref document: B2 Effective date: 20201216 |
    优先权:
    申请号 | 申请日 | 专利标题
    ES201930661A|ES2749689B2|2019-07-17|2019-07-17|DIFRACTIVE DEVICE FOR CHEMICAL AND BIOLOGICAL ANALYSIS|ES201930661A| ES2749689B2|2019-07-17|2019-07-17|DIFRACTIVE DEVICE FOR CHEMICAL AND BIOLOGICAL ANALYSIS|
    AU2020312756A| AU2020312756A1|2019-07-17|2020-07-06|Diffractive device for chemical and biological analysis|
    PCT/ES2020/070435| WO2021009397A1|2019-07-17|2020-07-06|Diffractive device for chemical and biological analysis|
    CA3144564A| CA3144564A1|2019-07-17|2020-07-06|Diffractive device for chemical and biological analysis|
    [返回顶部]