专利摘要:
Device and method of monitoring activity in fish. Device located in the operculum to monitor the metabolic state and well-being of fish through joint and simultaneous measures of respiratory rate and physical activity by means of a plurality of coplanar accelerations (x, y) and normal to the operculum (z axis). The substrate is flexible and covered with a waterproof coating, where an electronic circuit with acceleration sensor (208), a control unit (202), a microcontroller (206) configured to run at least one activity monitoring routine is located, a memory (210) and a battery (204). The data collection is carried out through an external unit (201) that can eventually process them totally or partially. These types of measurements can be processed together with other types of measurements, obtained from other environmental parameters sensors. (Machine-translation by Google Translate, not legally binding)
公开号:ES2725913A1
申请号:ES201830305
申请日:2018-03-28
公开日:2019-09-30
发明作者:Casas Enric Cabruja;Fantoba Manuel Lozano;Sánchez Jaume Pérez;Giner Josep Calduch;González Carlos Javier Sosa;Ballester Miguel Angel Ferrer;Nelson Juan Antonio Montiel;López Juan Manuel Afonso
申请人:Consejo Superior de Investigaciones Cientificas CSIC;Universidad de las Palmas de Gran Canaria;
IPC主号:
专利说明:

[0001]
[0002]
[0003]
[0004] OBJECT OF THE INVENTION
[0005]
[0006] The present invention is framed in the technical field of control and supervision technologies of the welfare state of animals, especially in aquaculture, through the monitoring of physical activity and respiratory rate.
[0007]
[0008] A device for monitoring physical activity and respiratory rate in fish is described. The device is configured to be installed in the operculum of the fish, and by means of an acceleration sensor record its accelerations in the plane of the operculum and in its normal direction to that plane.
[0009]
[0010] The method of monitoring the accelerations of the operculum allows to extract the respiratory rate of the fish and the movements resulting from swimming activity, jointly and simultaneously, in order to determine the metabolic and welfare state of the animal. The measurements obtained with the device can be complemented with measures of environmental parameters of the installation or culture medium, obtained both in real time and in a predictive way.
[0011]
[0012] BACKGROUND OF THE INVENTION
[0013]
[0014] Aquaculture is the only animal production sector with a wide margin of growth globally. Currently, aquaculture contributes 50% of the products derived from the sea, with this contribution expected to increase by about 80 million tons in 2050.
[0015]
[0016] In the last decade important advances have been made in the formulation of diets, diagnosis and prevention of diseases and genetic improvement programs. However, an urgent demand of the industry is the development and implementation of new biological monitoring systems for both the environment and animals in culture. The ultimate goal is to monitor the metabolic and / or health status of animals in culture remotely and non-invasively. This practice is necessary for the development of new cultivation techniques that guarantee animal welfare while maximizing the production efficiency in a scenario of global change, with an increase in temperature, acidification of seawater and a decrease in the concentration of dissolved oxygen in the water.
[0017]
[0018] The use of labels to identify or mark fish, as described in US4790090, is a method known for more than 25 years. In their initial version these tags were passive elements that simply identified the animal. These tags are usually attached to the body or some fin. In document UA70087, labels are described that are fixed in the operculum, but they are passive labels without any electronic element in them.
[0019]
[0020] More recently, electronic versions have appeared, in which the reading of the tag is carried out wirelessly by radiofrequency, but they are also limited to identifying each animal with a unique code, such as those described in document US6400338.
[0021]
[0022] One of the methods used to monitor the status of fish activity is based on recording by video cameras and image analysis (document RU2596498). This method is complex and difficult to use routinely in the open sea or with high crop densities.
[0023]
[0024] On the other hand, miniaturized acceleration sensors have been used for years to study the activity of people. In the publication "Activity recognition using cell phone accelerometers ", Kwapisz et al. (2010) describe an algorithm to detect the different activities of a person from the acceleration sensor located in his pocket. The procedure starts from the acceleration to detect the type of movement performed (running, walking, sitting, etc.). This algorithm is not compatible with the movement patterns of other living beings.
[0025]
[0026] Similarly, WO2015123373 refers to a system and method for detecting movement patterns in any type of object, animal or person by means of a device with a motion sensor that is attached to the individual. The system and method require classifying the type of movement according to a previously classified pattern. Classifying movement is not a sufficient condition to determine physical activity and respiratory rate, jointly and simultaneously, in fish.
[0027] In the publication "Accelerometer tags: detecting and identifying activities in fish and the effect of sampling frequency ', Broell et al. (2013) describe the use of implantable acceleration sensors in fish to monitor their activity. More specifically, in the publication It refers to an algorithm to detect the different activities of the fish such as the moment of feeding or escape.They use the acceleration obtained from an acceleration sensor located in the first dorsal fin by means of a Velcro®, without considering the respiratory rate.
[0028]
[0029] A similar approach is described in “Recent advances in telemetry for estimating the energy metabolism of wild fishes” (Metcalfe et al., 2016) to evaluate the metabolic activity of fish using acceleration sensors, without considering respiratory rate, and estimating consumption of oxygen depending on the dynamics of fish accelerations.
[0030]
[0031] DESCRIPTION OF THE INVENTION
[0032]
[0033] The present invention describes a device for controlling and monitoring the metabolic state and animal welfare, autonomously, remotely and individually monitoring physical activity and respiratory rate.
[0034]
[0035] Thus, the present invention allows to solve the technical problems of the state of the art of obtaining the frequency of breathing of the fish, and the physical activity, jointly and simultaneously; and to monitor the level of physical activity related to respiratory rate, swimming speed and oxygen consumption.
[0036]
[0037] The device comprises a substrate, for example, flexible, preferably a label, which in its simplest form comprises: an acceleration sensor; a control unit (microcontroller) configured to execute, partially or totally, an activity monitoring routine; a memory; A battery; an external unit of measure for downloading data, and eventually processing them; optionally, a transmitter for optical, electrical, magnetic or electromagnetic communication between the substrate fixed to the operculum of the fish and the external unit.
[0038]
[0039] The acceleration sensor is an inertial device that provides acceleration data on three orthogonal axes, called x, y, z; and that allows the detection of small opercular movements and the displacement of the fish, in the form of accelerations. Accelerations are stored in memory and processed, totally or partially, by the microcontroller in order to quantify the respiratory rate and the level of physical activity.
[0040]
[0041] The microcontroller provides the computing power to execute, in whole or in part, the activity monitoring routine that allows the calculation of physical activity and respiratory rate. For this it has multiple modules that allow the control, storage, processing, and communication of the data generated by the acceleration sensor. The microcontroller provides a communication interface with the external unit that allows programming, configuration and downloading of the data generated by the acceleration sensor. It also provides an internal interface to communicate with said acceleration sensor.
[0042]
[0043] The memory is preferably non-volatile and is used to store the activity monitoring routine, the acquired accelerations and the data resulting from the analysis of the physical activity and respiratory rate. The microcontroller has an energy management unit to minimize battery consumption during periods of inactivity. The microcontroller has an event planning unit for the temporary execution of the experiments (data acquisition of the acceleration and processing sensor, total or partial). Events can be programmed into memory or triggered from the external unit.
[0044]
[0045] The battery is, in an exemplary embodiment, rechargeable and high performance to provide sufficient power to the rest of the components of the electronic circuit (acceleration sensor, microcontroller, memory, and the transmitter-receiver when present) during the life of the device .
[0046]
[0047] The substrate is placed temporarily or permanently in the operculum of the fish to be monitored. The implantation in the operculum can be done for example by means of perforations for its fixation, or by a clamp mechanism, or by means of an adhesive substance. Through the acceleration sensor, the movements of the fish when swimming are recorded, as well as the movements of the operculum, jointly and simultaneously, in the form of accelerations. Both movements, which are fused into a single multidimensional signal, are separated by processing the data acquired by the acceleration sensor and used to determine the swimming activity and the frequency at which the fish breathes.
[0048] The data obtained by the acceleration sensor located in the operculum are stored in memory and subsequently processed totally or partially by the control unit. To read the data of the device, for example, the fish is captured, the substrate is removed and the information stored in memory is downloaded onto a computer through the external unit; or, without removing the device, the data is downloaded by optical, electrical, magnetic or electromagnetic communication.
[0049]
[0050] The activity monitoring routine is based on the calculation of the opercular frequency in the normal direction to the plane of the operculum, for example, z axis. In addition, a statistical calculation of the coplanar accelerations to the operculum, for example, xy plane, is performed to obtain an indicator of fish activity.
[0051]
[0052] If the fish shows symptoms of stress, malnutrition or disease, among others, it will breathe with an abnormal frequency, and its behavior may be affected by erratic movements of high activity or a state of rest. After a study of the behavior, which is specific to each species, crop condition and development phase, it is possible to establish a comfort function, so that from the acceleration sensor readings, respiratory rate and physical activity are extracted , determine the metabolic state and degree of animal welfare. This determination may eventually be refined through readings of environmental parameters, obtained both in real time and in a predictive way by other sensors.
[0053]
[0054] As described previously, there are commercial labels to identify fish, but in all cases they are passive and only transmit an animal identification code. There are also research groups that work with acceleration sensors in fish, but only record swimming movement patterns, without taking into account the respiratory rate. Thanks to the device of the present invention, which records the swimming and respiratory movement, jointly and simultaneously, with a single sensor device, reliable measurements of the degree of well-being of the fish can be obtained.
[0055]
[0056] In an exemplary embodiment, the electronic circuit components are mounted on the preferably flexible substrate by means of encapsulation techniques, such as inverted circuit technology, wire welding or surface mounting. Also in an exemplary embodiment, the waterproof coating is made of insulating epoxy to prevent water contact with the components of the electronic circuit.
[0057] In an exemplary embodiment, the control unit is configured to fully execute an activity monitoring routine comprising filtering and analysis of the multidimensional signal obtained by the acceleration sensor. The analysis determines the respiratory rate and physical activity of the fish. The external unit downloads the data obtained in the analysis.
[0058]
[0059] In an exemplary embodiment, the control unit is configured to partially execute an activity monitoring routine. In the external processing unit, after downloading the data, these are filtered and processed to determine respiratory rate and physical activity.
[0060]
[0061] In another exemplary embodiment, the control unit is configured to acquire the accelerations that are stored in memory. In the external processing unit, after downloading the data, these are filtered and processed to determine respiratory rate and physical activity.
[0062]
[0063] DESCRIPTION OF THE DRAWINGS
[0064]
[0065] To complement the description that is being made and in order to help a better understanding of the characteristics of the invention, according to a preferred example of practical realization thereof, a set of drawings is accompanied as an integral part of said description. where, for illustrative and non-limiting purposes, the following has been represented:
[0066]
[0067] Figure 1.- Shows a device implanted in the operculum of the fish.
[0068]
[0069] Figure 2.- Shows the electronic circuit of the device, the accelerometer, the battery and an interface of an external device has been represented to show how communication (data sending, programming) between the substrate and the external device is carried out.
[0070]
[0071] Figure 3.- It shows a diagram describing the method of monitoring the activity of the fish by their breathing frequency and their activity index.
[0072] PREFERRED EMBODIMENT OF THE INVENTION
[0073]
[0074] An example of embodiment of the present invention is described below with the aid of Figures 1 to 3.
[0075]
[0076] The substrate of the fish monitoring device of the present invention is configured to be placed in the operculum of a fish to determine different types of physical activity and respiratory rate. For example, in figure 1 the substrate of the device (102) already located in the operculum of a fish (101) is shown and the three orthogonal axes (x, y, z) in which the accelerations are measured are shown. In this example, the acceleration measures in the xy plane (103) which is coplanar with the operculum are used for monitoring, from which the swimming movement of the fish is extracted; and, in addition, the acceleration measures on the z axis (104), which is normal to the operculum, from which the respiratory rate of the fish is obtained based on the movements of the operculum.
[0077]
[0078] Figure 2 shows the scheme of the electronic circuit of the device (102). This electronic circuit is installed on a flexible substrate and protected from water by a waterproof coating.
[0079]
[0080] The electronic circuit comprises:
[0081] a) an acceleration sensor (208) intended to acquire acceleration data of the fish to which the device is attached in the plane of the operculum, xy (103) and in the normal direction to the operculum, z axis (104);
[0082] b) a control unit (202), preferably microcontroller, which has all the necessary subsystems: communication, serial bus, program and data memory, power consumption control system, among others. This control unit is connected to the acceleration sensor (208); and it is configured to receive the data of said acceleration sensor, for example, via the I2C interface of the bus (207), store it for example in a non-volatile memory (210) and transmit it to an external device (201). The control unit (202) is configured to execute at least one routine for monitoring physical activity and respiratory rate (209);
[0083] c) a battery (204) that powers the acceleration sensor (208) and the control unit (202) with all its subsystems.
[0084]
[0085] In an exemplary embodiment of the invention, the control unit (202) includes a communication interface (205) with an external unit (201). This communication is done by an optical, electrical, magnetic and / or electromagnetic transmitter / receiver. The activity monitoring routine is executed, totally or partially, in the external unit (201).
[0086]
[0087] The interface (205) allows the programming of the control unit (202) by the external unit (201).
[0088]
[0089] The control unit (202) can also be configured to execute, in a microprocessor (206), totally or partially, the activity monitoring routine. The control unit (202) also executes an experiment planning routine. This routine allows you to control the timing and synchronization of the data acquisition of the acceleration sensor (208) for a certain period of time.
[0090]
[0091] Also, the control unit includes an energy manager (211) to reduce energy consumption during the periods of execution of the data shots. The subsystem for computing acquired acceleration data (209) comprises a routine of data analysis to determine respiratory rate and physical activity.
[0092]
[0093] Preferably the battery (204) is of minimum size and weight but of great performance to allow long periods of execution of the data analysis routine.
[0094]
[0095] In a preferred embodiment of the invention, the electronic circuit is arranged in a package as small as possible to minimize the weight and size of the device.
[0096]
[0097] In a preferred embodiment the electronic components are surface mounted, and are chosen from the minimum size. The battery can be button type, rechargeable with minimum diameter. The support substrate for the entire system is preferably made of a flexible organic material to allow the bending of one of its ends on itself in order to connect the button-type battery on both sides.
[0098]
[0099] Figure 3 shows the different stages of the activity monitoring routine that includes the procedure for calculating physical activity and respiratory rate in fish proposed in the present invention. The first necessary step is to obtain the acceleration data (301) in the three orthogonal axes x, y, z (ax, ay, az) either from the accelerometer (208), from memory (210) or from the external unit (201 ). For example, the coplanar accelerations ac to the operculum are contained in the xy plane, and the acceleration normal to the operculum corresponds to the z axis. These data are obtained with the acceleration sensor (208) or are stored in memory (210) after being acquired, or are available in the external unit (201).
[0100]
[0101] In an exemplary embodiment, the acceleration data in the normal direction an to the operculum, for example, the accelerations of the z-axis (az), their average value (303) is subtracted and, subsequently, a number count is made and distance from maximums, and thus determine the respiration rate (305). Also, to the data of coplanar accelerations ac, for example, the accelerations of the x axis (ax) and y (ay), their average value is also subtracted, respectively (302). From the results obtained, an index of physical activity is determined (304).
[0102]
[0103] Finally, a step of storing (306), in memory (210) or in the external unit (201), the data obtained from respiration rate (305) and activity index (304) is performed.
[0104]
[0105] In a preferred embodiment, a certain time interval is selected and during intervals of said times representative values of each set of acceleration sensor samples obtained on each axis are calculated with a sampling frequency.
权利要求:
Claims (7)
[1]
1. - Device for controlling and monitoring the metabolic state, activity and well-being of fish, characterized in that it comprises a substrate (102) with an electronic circuit for the joint and simultaneous measurement of the coplanar and normal accelerations at the operculum of the fish and a coating waterproof covering the electronic circuit, being configured to temporarily or permanently join the operculum of the fish that you want to control and monitor physical activity and respiratory rate during periods of intake and no feeding throughout the day, and the electronic circuit comprises :
- an acceleration sensor (208) intended to acquire the accelerations of the operculum of the fish, - a control unit (202) connected to the acceleration sensor (208) and containing at least one microcontroller (206) configured to execute at least one activity monitoring routine, a memory (210) configured to store the data acquired by the acceleration sensor (208) and / or the data generated by the microcontroller (206) when executing an activity monitoring routine,
- a battery (204) connected to the control unit (202), and
The substrate (102) is configured to establish communication with an external device (201) to which the data acquired by the acceleration sensor (208) and / or the data generated by the microcontroller (206) are sent.
[2]
2. - Device for controlling and monitoring the metabolic state, activity and well-being according to claim 1, characterized in that the control unit (202) comprises a communication interface (205) configured to establish communication with the external device (201) for sending the data obtained by the acceleration sensor (208) and / or the data stored in the memory (210).
[3]
3. - Device for monitoring and monitoring the metabolic state, activity and welfare of fish according to claim 1, characterized in that the control unit (202) comprises a communication bus (207) linked to the acceleration sensor (208) and configured to establish communication with said acceleration sensor (208).
[4]
4. - Device for controlling and monitoring the metabolic state, activity and welfare of fish according to claim 1, characterized in that it additionally comprises an energy manager (211) linked to the microcontroller (206) and the battery (204) and configured to control the rest and data collection times during the execution of the activity monitoring routine.
[5]
5. - Device for controlling and monitoring the metabolic state, activity and welfare of fish according to claim 1, characterized in that it additionally comprises an external unit (201) configured to receive and process the data sent by the control unit (202).
[6]
6. - Device for controlling and monitoring the metabolic state, activity and welfare of fish according to claim 1, characterized in that the control unit (202) comprises at least one data computing chip (209) configured to process the data of the acceleration sensor (208).
[7]
7. - Procedure for control and supervision of the metabolic state, activity and welfare of fish with a device for control and supervision of the metabolic state, activity and welfare of the fish as described in any one of claims 1 to 6, characterized in that It comprises executing a monitoring routine in which the following steps are performed:
- measure with acceleration sensor (208) the accelerations in three orthogonal axes, two of them coplanar to the opercle x axis, y ( ax, ay), and a third normal to the opercle z axis ( az), jointly and simultaneously ;
- calculate at predetermined time intervals, the representative values of a plurality of samples of the acceleration sensor (208) obtained on each x, y, z axis with a predetermined sampling frequency;
- extract the respiration frequency from the accelerations on the z axis (az);
- calculate the physical activity index from the accelerations in the x, y ( axay) axes.
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同族专利:
公开号 | 公开日
WO2019185965A1|2019-10-03|
EP3779849A1|2021-02-17|
ES2725913B2|2020-03-27|
EP3779849A4|2021-12-08|
引用文献:
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US6400338B1|2000-01-11|2002-06-04|Destron-Fearing Corporation|Passive integrated transponder tag with unitary antenna core|
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KR20130005484A|2011-07-06|2013-01-16|코리아컴퓨터|Rfid tag for displaying history of marine products and method of the same|
EP3104777B1|2014-02-14|2020-11-11|3M Innovative Properties Company|Activity recognition using accelerometer data|
RU2596498C1|2015-04-14|2016-09-10|Федеральное государственное бюджетное учреждение науки "Уральский научно-практический центр радиационной медицины" Федерального медико-биологического агентства России |Method of non-invasive lifetime estimation of physiological state of fish|CN112568150A|2020-12-11|2021-03-30|浙江大学|Device and method for measuring swimming capacity, oxygen consumption rate and ammonia discharge rate of aquatic animals|
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优先权:
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ES201830305A|ES2725913B2|2018-03-28|2018-03-28|DEVICE AND METHOD OF MONITORING ACTIVITY IN FISH|ES201830305A| ES2725913B2|2018-03-28|2018-03-28|DEVICE AND METHOD OF MONITORING ACTIVITY IN FISH|
PCT/ES2019/070205| WO2019185965A1|2018-03-28|2019-03-27|Device and method for monitoring activity in fish|
EP19776863.3A| EP3779849A4|2018-03-28|2019-03-27|Device and method for monitoring activity in fish|
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