• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    Device for the retention and exploration of eyes of rats and mice that includes a grid formed by curved telescopic rods (1) and straight (22) forming a cylinder, with a rounded end for the head of the animal and an adjustable access, whose size it can be modified to immobilize the animal. For the exploration of the eyes, the device also includes a multimedia and optical system. To protect the animals, the central grid of the grating can be covered internally with an elastic and transparent silicone in which sensors of movement and physiological functions of rats and mice can be inserted. It is a useful device to immobilize animals and to study spherical, astigmatic and other aberrations in the eyes of experimental animals. (Machine-translation by Google Translate, not legally binding)
    公开号:ES2699413A1
    申请号:ES201800205
    申请日:2018-09-10
    公开日:2019-02-11
    发明作者:Vilaboa Ricardo Bernardez；Del Portal Josefina María Illera；Granado Gema Silvan；Del Portal Juan Carlos Illera；Ramos Sara Caceres；Illera María Corres；Lanchares Juan Carlos Cuevas；Ruiz José María Ruiz
    申请人:Universidad Complutense de Madrid；
    IPC主号:
    专利说明:

    [0001]
    [0002] Device for the retention and exploration of eyes in rats and mice.
    [0003]
    [0004] Sector of the Technique
    [0005]
    [0006] The invention falls within the technical field of Optometry and Veterinary, more specifically refers to devices for the retention of rats and mice to achieve the precise measurement of the structure and function of their eyes.
    [0007]
    [0008] State of the art
    [0009]
    [0010] In the usual exploration of a rat and / or mouse, different boxes of different plastic materials are used where the experimental animals are introduced, to perform procedures. These boxes are usually translucent because they do not require any specific optical property to perform the actions that are carried out on these animals, used in experimentation. In this work, often, inoculations and other minimally invasive actions are required that require the immobilization of the animal. In these examinations, eye examinations of the rat and / or the laboratory mouse are not usual.
    [0011] Rats and mice, like humans, have two types of light receptors, cones, sensitive to light and color, and rods. The rats differ by the number of cones in the retina and by the type of receptor. In the human being there are 3 types of colored cones, that's why the vision is trichromatic and in the rat only two, so their vision is dichromatic. The perception of color in these animals is weaker than in humans.
    [0012]
    [0013] Having the eyes on both sides of the head allows them to obtain a large field of vision, but they get less field of binocular vision and less depth vision than the human being. The rats use the parallax movement to calculate that depth and with vertical head movements get to assess the distance that separates them from the point to reach.
    [0014]
    [0015] On the other hand, several techniques of observation of the eye such as ophthalmoscopy or retinoscopy serve to check its external and internal structures. The veterinary ophthalmologist has sophisticated equipment and expensive for this observation, necessary to know the state of the eye of animals and intervene, if necessary, to solve problems that may affect them, preventing the correct vision of one or both eyes .
    [0016]
    [0017] For the study of the eyes of rats and / or experimental mice, devices are needed to retain the animal to move the head as little as possible, without causing pain and respecting all established rules on the management of experimental animals and, In addition, that meets the conditions and elements necessary to analyze your eyes.
    [0018]
    [0019] There are some devices to hold mice and laboratory rats. Generally, they are made of methacrylate, have a prismatic or cylindrical shape, and can have a certain plasticity to suit different sizes of mice, if they are designed for mice, or rats, if they are designed for rats. They can also have openings or slots to access the immobilized animal and practice certain interventions. An example is patent application JP2005111108A, which refers to a device that includes a rectangular base in the center of which a cylindrical main body is incorporated, arranged parallel to the sides of the base, where the trunk of the animal body is immobilized. This main body of the device is open at one end and closed at the opposite end, and has openings in the lower part, on both sides, to be able to extract the four limbs of the animal that are immobilized on the base. It also has elements to immobilize the tail of the animal, adaptable by screws, and the possibility of including grooves along the main body of the device to access different parts of the body of the animal.
    [0020] However, in order to analyze the eyes of mice and laboratory rats, it is necessary not only to have the trunk of the animal immobilized, but to keep its head as immobile as possible, while leaving the eyes uncovered so that they can be analyzed without any optical barriers being interposed. .
    [0021] Detailed description of the invention
    [0022] Device for the retention and exploration of eyes in rats and mice.
    [0023] The present invention proposes a biosensitive device in the form of a cylindrical grid whose dimensions can be modified depending on the size of the animal, in order to be able to explore the eyes, and which can be covered in its interior by a silicone in which sensors are incorporated to determine distances of measurement and physiological values of the animal. The device also incorporates the necessary accessories to examine the eyes of rats and mice.
    [0024] On the one hand, the device has the ability to retain the rat or experiment mouse. For this, the grating is formed by rods of decreasing thicknesses, the rods being hollow, so that the rods of smaller thickness can be introduced inside the rods of greater thickness as a telescope. That is to say, the grid is formed, in the first place, by:
    [0025] - Long telescopic rods that are determined three parts:
    [0026] - central framework: formed by straight telescopic rods composed of successive straight rods placed as a telescope, at least two in two longitudinally, which can be collected by fitting one in the previous, which reduces its length to reduce the length of the grating, arranged in space creating a cylindrical body;
    [0027] - rounded end: curved rods of decreasing diameter that start from the circumference of one of the bases of the cylinder of the central framework, creating a rounded structure, and that end up joining in a ring;
    [0028] - adjustable access: straight rods that start from the circumference of the base of the cylinder opposite the rounded end, joining a curved telescopic rod disposed forming a circle at the free end of the straight rods of the adjustable access, said curved telescopic rod constituting a ring terminal; - curved telescopic rods: successive curved rods, placed at least two in two longitudinally, which can be collected by fitting one in the previous one, and which are placed perpendicular to the straight telescopic rods of the central framework, thereby reducing their length to decrease the width of the grate.
    [0029] The curved rod of greater thickness of each of the curved telescopic rods of the central framework is joined by a point to a straight rod of the straight telescopic rods of the central framework to maintain its position within the framework of the grid.
    [0030] The result of this conjunction of straight and curved telescopic rods of the central framework is a cylinder; to be able to modify the length and width of the grate, understanding as such the cylinder diameter, said cylinder can be adapted to the size of each rat or mouse that is desired to immobilize.
    [0031] Once the diameter and / or the length of the grid has been modified to adapt it to the size of the animal under study, the movements of the animal itself could change the size of the grid in any of its dimensions. To avoid this problem, one aspect of the invention relates to the incorporation of at least one key to block the movements of the curved telescopic rods in width (cylinder diameter) and at least one key to block the movements of the telescopic rods. the telescopic rods straight from the central framework in length.
    [0032] To introduce the animal to be explored inside the cylinder formed by the straight telescopic rods and the curved telescopic rods, both of the central framework, the adjustable access has been designed in one of the bases of the cylinder. This base is open and, once the animal is introduced, its closure is made by the terminal ring, which can retain the tail of the rat or the mouse. To block said closure, the invention includes a key in the terminal ring.
    [0033] The opposite end of the cylinder is rounded to fit the head of the animal and prevent the movement of its head. For this, curved rods are inserted that start from the circumference of the base of the cylinder creating a rounded structure and are joined to a ring in such a way that the rods can slide through the ring when increasing or decreasing the diameter of the cylinder. To facilitate this plasticity, the diameter of the rods progressively decreases from the base of the cylinder to the ring.
    [0034] In a preferred embodiment, the structure of the grid is made of plastic or metal material, preferably a light metal, where light metals are understood to be those whose relative density is equal to or less than 4.5 g / cm3, such as alloys of aluminum, titanium or magnesium. Telescopic rods can be circular, square, rectangular, triangular and / or elliptical; preferably, the section is circular.
    [0035] On the other hand, in order not to damage the animal, the structure created by the rods is preferably covered with elastic silicone on the inside face of the grid, except at the rounded end where the head of the animal under study will be housed. Preferably, transparent silicone is chosen. To be able to elongate and shrink according to the dimensions of the grid, a silicone is selected whose structure is extensible and allows the sliding of all the rods, in addition, it is selected 100% nibbled, antibacterial and free of BPA; for this there are silicones with approved quality for use in contact with food, so that they do not pose a danger to experimental animals.
    [0036] To be able to perform the exploration of the eyes of rats and mice, on the outside of the grating, at the rounded end of the same, there is a multimedia and optical system, pointing to the eyes of the mouse or the rat. The multimedia and optical system includes:
    [0037] - a digital camera with which to take photographs and videos, which includes:
    [0038] - elements for the storage of information,
    [0039] - a lens with optical zoom;
    [0040] - a set of lenses;
    [0041] - an observation head, which includes:
    [0042] - a light bulb,
    [0043] - a prism;
    [0044]
    [0045] - orange light detection sensors that reflect the eyes of the animal when they receive the light from said light source.
    [0046]
    [0047] The digital camera can be equipped with movement, so that said movement can be executed in 360 ° at will or programmed in a fixed sequence, in all directions, while the camera takes still and / or moving images that will store in a appropriate device, to analyze it later with the most appropriate computer program. As an example of elements for storing information, it can be high capacity SD cards.
    [0048]
    [0049] On the other hand, the light source will preferably be a LED type bulb. In a preferred embodiment of the invention, the light reflected in the eyes of the animals passes through the set of lenses and the system includes the elements necessary to automatically determine the particular lens of the focusing assembly with precision, in the focal point of the camera , the reflected light. The light source is included in an observation head that also includes a prism of deviation of the light. All this to examine the eye of the animal, for example, to measure the fundus and the structures attached to the eye.
    [0050]
    [0051] In addition, the device includes sensors for determining measurement distances between the eyes of the animal and the multimedia and optical system, and sensors for measuring physiological values of the animal, such as heart rate control sensors. The device incorporates said sensors in the silicone sheet that covers the sides of the structure, to detect different movements of the animal and, especially, the approach to the multimedia and optical system to photograph or video record, automatically, the eyes of the mouse or the rat, so that defects such as spherical, astigmatic and other aberrations can be detected in the eye of the animal (myopia, hyperopia, astigmatism and aberrations of another order such as coma).
    [0052]
    [0053] The use of this device allows, in the first place, the immobilization of the animal and, secondly, a rapid analysis of the eyes of rats and mice, as well as the storage of the information for further analysis.
    [0054]
    [0055] Description of the figures
    [0056]
    [0057] The following characteristics of the present invention are shown in an illustrative and non-limiting manner in the attached drawings:
    [0058]
    [0059] Figure 1. Scheme of the grid
    [0060]
    [0061] A diagram of the biosensitive device is shown with a grid formed by a central framework with curved telescopic rods (1), formed by curved rods (11) of the central framework, and long telescopic rods (2) formed by straight telescopic rods (22), formed in turn by straight rods (222) of the central framework, attached to a rounded end of curved rods of decreasing diameter (3), which are joined in a ring (4), and an adjustable access through which the animal is introduced, ending in a terminal ring (5) that can be reduced to the size of the tail of the animal. The grid has 3 keys (9) to retain the closure or allow the opening of the set of rods and the terminal ring.
    [0062]
    [0063] Figure 2. Multimedia and optical system
    [0064] The multimedia and optical system is included in a box (10) and is connected to an external hard disk by means of a USB plug (100). The box (10) includes a digital camera (6) and has clips (7) with which it is hooked to the curved rods of decreasing diameter that make up the rounded end of the grid. By means of the digital camera (6) the images formed by the projection of light from the focus (12) included in the observation head (8), which also includes a prism (13), and a lens box including the set of lenses (14).
    [0065]
    [0066] Embodiment of the invention
    [0067]
    [0068] The present invention is further illustrated by the following example, which is not intended to be limiting of its scope.
    [0069]
    [0070] Example 1. Device for mice
    [0071]
    [0072] A biosensitive device with 3 curved telescopic rods (1) combined with 28 long telescopic rods (2) made of polyvinyl chloride was manufactured. The straight telescopic rods (22) of the central framework were designed with 3 hollow straight rods (222) each 4 cm long, with circular section, 2.2 mm diameter, 2 mm and 1.8 mm, respectively, separated 4 mm between them. The curved telescopic rods (1) were made by 6 curved rods (11) of 3 cm each with decreasing diameters from 3 mm. Each curved telescopic rod (1) was welded to one of the straight rods (222) that make up a straight telescopic rod (22) of the central framework, so that the entry of that rod into the thicker anterior would be minimized.
    [0073]
    [0074] For the rounded end, 28 curved rods of decreasing diameter (3) were used, starting from each straight telescopic rod (22), joined to a ring (4) by means of rings that allow the sliding of the long telescopic rods (2) for dwarf or enlarge the diameter of the cylinder constituted by the central framework.
    [0075]
    [0076] The adjustable access was made with straight rods attached to a terminal ring (5) constituted by a curved telescopic rod that, when introduced inside itself, is able to close.
    [0077] On the other hand, a stop was included in each of the rods that make up the different telescopic rods so that the smaller diameter is held within the light of the rod of immediately superior diameter, so that each of the telescopic rods is keep as a whole.
    [0078]
    [0079] In the telescopic rods, a locking key (9) was included in each of the insertions of a rod within the previous one. Another key (9) was included in the terminal ring.
    [0080]
    [0081] The entire central framework was covered on the inside with food-grade elastic silicone so that it was not harmful to the animals. SILAG40 silicone sheet from the Lagon rubber house was used and movement sensors were inserted into the silicone itself.
    [0082]
    [0083] In the rounded end rods, the multimedia and optical system that was manufactured inside a box (10) in polyvinyl chloride with a digital camera (6) with high capacity SD card, with the possibility of taking photos was fixed and videos in HD format of high resolution and with a lens with optical zoom of 20X. In front of the camera, a light projection system with cold light LED was installed in the observation head (8). Within the multimedia and optical system, there are a set of lenses (14), a prism (13) and the necessary sensors to verify that the image of the eye is reflected in the focus of the lens as clearly as possible. A USB plug (100) was included on the outside of the box (10) where You can connect any device to store or analyze the data obtained by the digital camera (6).
    [0084]
    [0085] Example 2. Rat device
    [0086]
    [0087] A biosensitive device similar to that of Example 1 was manufactured, but with larger dimensions and using aluminum for the different types of rod and for the keys. Four curved telescopic rods (1) combined with 48 long telescopic rods (2) made of aluminum were used. The straight telescopic rods (22) of the central framework were designed with 2 hollow straight rods (222) of 12 cm length each, with square section, side of 3.2 mm and 3 mm, respectively, separated 3.5 mm between they. The curved telescopic rods (1) were made by 6 curved rods (11) of 6 cm each with decreasing sides from 4 mm. Each curved telescopic rod (1) was welded to one of the straight rods (222) that make up a straight telescopic rod (22) of the central framework, so that the entry of that rod into the thicker anterior would be minimized.
    权利要求:
    Claims (10)
    [1]
    1. Device for the retention of rats and mice that includes:
    - a grid formed by curved telescopic rods (1) and long telescopic rods (2) in which the long telescopic rods (2) determine three parts:
    - central framework including straight rods (222) successively placed as a telescope, at least two in two longitudinally, which can be collected by fitting one in the previous, which reduces its length to reduce the length of the grid, arranged in space creating a cylindrical body;
    - rounded end formed by curved rods of decreasing diameter (3) that start from the circumference of one of the bases of the cylinder of the central framework, creating a rounded structure, and that end up joining in a ring (4);
    - adjustable access formed by straight rods starting from the circumference of the base of the cylinder opposite the rounded end, joining a curved telescopic rod disposed forming a circle at the free end of the straight rods of the adjustable access, said curved telescopic rod constituting a terminal ring (5);
    and the curved telescopic rods (1) include successive curved rods (11) placed, at least, two in two longitudinally, which can be collected by fitting one in the previous one, and which are placed perpendicular to the straight telescopic rods (22) of the framework central, which reduces its length to reduce the width of the grid.
    [2]
    Device according to claim 1, in which the grid is made of plastic material or light metal, light metal being that whose relative density is equal to or less than 4.5 g / cm3.
    [3]
    Device according to any one of the preceding claims, in which the central framework is internally covered with transparent silicone, elastic and not harmful to animals.
    [4]
    Device according to claim 3, in which motion sensors and / or physiological functions of the animals are included in the silicone of the central framework.
    [5]
    Device according to any of the preceding claims which includes keys for locking the straight telescopic rods (22), the telescopic rods (1) and / or the terminal ring (5).
    [6]
    6. Device for scanning the eyes of rats and mice that includes a retention device as defined in any of the preceding claims and a multimedia and optical system that includes:
    - a digital camera with:
    - elements for the storage of information,
    - a lens with optical zoom;
    - a set of lenses (14);
    - an observation head (8), which includes:
    - one light bulb (12),
    - a prism (13);
    - orange light detection sensors that reflect the eyes of the animal when they receive the light from said light source.
    [7]
    Device according to claim 6, wherein the digital camera is equipped with a movement of up to 360 °.
    [8]
    Device according to any of claims 6-7 in which the light source is LED type.
    [9]
    Device according to any of claims 6-8 which includes elements for automatic focusing of the animal's eye images, in the digital camera (6).
    [10]
    Device according to any of claims 6-9 which includes the multimedia and optical system in a box (10) with clips (7) to join the box (10) to the grille of the device.
    类似技术:
    公开号 | 公开日 | 专利标题
    US10034606B2|2018-07-31|Optical equipment for observation of the iridocorneal zone, methods of measuring and/or evaluating the iridocorneal zone
    ES2743200T3|2020-02-18|System to visualize areas of the body
    ES2754310T3|2020-04-16|Aid procedure for determining the vision parameters of a subject
    KR102028869B1|2019-10-04|Components, computer programs, systems and kits for judging corrective lenses
    ES2341079A1|2010-06-14|Equipment for infrared vision of anatomical structures and signal processing methods thereof
    ES2850574T3|2021-08-30|Adapter for fundus imaging device
    ES2706300T3|2019-03-28|Method for the acquisition of optical coherence tomography image data of the retina tissue of an eye of a human subject
    US20180206720A1|2018-07-26|Head-mounted indirect opthalmoscope camera
    ES2887355T3|2021-12-22|Determination of the azimuthal orientation of a patient's eye
    ES2699413B2|2019-07-01|Device for retention and examination of eyes in rats and mice.
    ES2329396T3|2009-11-25|METHOD AND SYSTEM FOR MEASURING Tear Fluid.
    ES2556472T3|2016-01-18|Ophthalmic lens holder for centering device
    ES2882792T3|2021-12-02|Display device of a digital image
    EP3731731A1|2020-11-04|Multiple off-axis channel optical imaging device with rotational montage
    ES2535194B1|2016-02-16|Smartphones adapter to a direct ophthalmoscope or otoscope
    CN108093156B|2020-11-13|Image capturing apparatus
    ES2726720T3|2019-10-08|TCO image modification
    KR200476846Y1|2015-04-10|Fundus image-taking interface apparatus
    CN102525654B|2016-09-07|The Head And Face accurate angle fixer of medical photography
    US9427145B2|2016-08-30|Ophthalmologic measurement apparatus, method and program of controlling the same
    ES2685054A1|2018-10-05|Device for objective technique of estimation of accommodative delay |
    ES1263065U|2021-03-18|Device for objective technique of estimation of accommodative delay of the eye |
    BR202020016196U2|2022-02-15|APPARATUS FOR OPHTHALMOLOGICAL EXAMINATION OF THE ANTERIOR SEGMENT AND FUNDO OF THE EYE WITH ELECTRONIC APPARATUS COUPLING MEANS WITH CAMERA AND TOWER FOR COUPLING ACCESSORY LIGHTING
    US9618339B2|2017-04-11|Spirit level
    ES2346390B1|2011-08-04|PROCEDURE FOR MICRO ESTEREOSCOPIC PHOTOGRAPH OF RETINA.
    同族专利:
    公开号 | 公开日
    ES2699413B2|2019-07-01|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    US3094101A|1962-04-02|1963-06-18|Ann L Porter|Apparatus for restraining animals|
    US3625185A|1970-05-28|1971-12-07|Rauland Corp|Adjustable device for limiting the movement of laboratory animals|
    US4770127A|1986-10-08|1988-09-13|Volk Seymour A|Animal restraining apparatus|
    US5320069A|1992-04-28|1994-06-14|The United States Of America As Represented By The Secretary Of The Army|Small animal restraint device|
    US5339772A|1993-11-22|1994-08-23|Petra Abell|Small animal restrainer|
    US20080076989A1|2006-09-21|2008-03-27|Starr Life Sciences Corp.|Tail Mounting Clip for Securely Mounting Sensor to Tail and a Tail Mounted Pulse Oximetry Sensor System Using Same|
    法律状态:
    2019-02-11| BA2A| Patent application published|Ref document number: 2699413 Country of ref document: ES Kind code of ref document: A1 Effective date: 20190211 |
    2019-07-01| FG2A| Definitive protection|Ref document number: 2699413 Country of ref document: ES Kind code of ref document: B2 Effective date: 20190701 |
    优先权:
    申请号 | 申请日 | 专利标题
    ES201800205A|ES2699413B2|2018-09-10|2018-09-10|Device for retention and examination of eyes in rats and mice.|ES201800205A| ES2699413B2|2018-09-10|2018-09-10|Device for retention and examination of eyes in rats and mice.|
    [返回顶部]