PROCEDURE FOR PREPARING MONOSACCHARIDE SUGARS FROM SOLID URBAN RESIDUE (Machine-translation by Googl

专利摘要:
Procedure to prepare monosaccharide sugars from urban solid waste. The present invention relates generally to systems and methods for forming monosaccharides from a solid waste mixture. An integrated process is provided to classify a solid waste mixture to generate several streams rich in recyclable materials including one or more plastic streams and a bioresiduous stream enriched in cellulosic compounds and comprising non-fermentable components. The bioresidue stream is pretreated under conditions of high pressure and temperature and then contacted with an enzyme source comprising cellulase, in which a certain part of the non-fermentable material present in the solid waste mixture is removed from the process by wet classification methods during or after enzymatic hydrolysis. (Machine-translation by Google Translate, not legally binding)
公开号:ES2688105A1
申请号:ES201730446
申请日:2017-03-28
公开日:2018-10-30
发明作者:Quang A. Nguyen；Ana Isabel VICENTE GARCÍA；Vanesa RAMOS GARCÉS；Ignacio CARVAJO LUCENA；Cristina Montejo Mendez
申请人:Abengoa Bioenergia Nuevas Technologias SA；
IPC主号:

专利说明:

5The field of the invention generally relates to a method for thesolid waste fractionation and for the production of useful products and therecovery of recyclable materials from different fractions. Plusparticularly, the process of the present invention relates to
10 fractionation of a solid waste mixture to provide a clean bio-waste stream suitable for conversion into monosaccharides and to provide recyclable streams that include high density polyethylene plastic and polyethylene terephthalate plastic. 15 STATE OF THE TECHNIQUE
Commercial, industrial and residential consumers generate large amounts of solid waste (for example, urban solid waste ("MSW")) that must be handled and disposed of in an environmentally responsible manner. By way of
20 Traditionally, MSWs have been eliminated by landfill disposal or incineration. However, these methods to dispose of the residual product contaminate the soil, water and air and require the use of land that could be used for other purposes.
The MSW comprises significant amounts of recyclable material including components such as cellular organic bio-wastes (such as food waste, garden waste, wood, paper and cardboard), plastic, glass, ferrous metals, and non-ferrous metals (such as aluminum). RSU classification operations to recover the different components are known in the art but
30 such known methods are typically ineffective. The prior art bio-waste fractions are typically impure and contaminated with various components, such as enzymatic hydrolysis and fermentation inhibitors, which normally convert these cellulosic bio-waste fractions into unsuitable for conversion into monosaccharides and fermentation products.
35 optional at an acceptable rate and commercial performance. For this reason, the methods


RSU fractionation of the prior art generally recovers value of organic bio-waste by incineration (energy recovery), gasification (by pyrolysis), or composting.
Therefore, there is a need for systems and methods to form cellulosic bio-wastes from a mixture of solid wastes and that said cellulosic bio-wastes have sufficient purity to allow commercially acceptable rates of conversion into monosaccharides by enzymatic hydrolysis. BRIEF DESCRIPTION OF THE INVENTION
In one aspect of the present invention, a method for preparing monosaccharide sugars from a mixture of urban solid waste is provided. The method comprises classifying the solid waste mixture into: an optional first stage (a) where manual or mechanical triage can be performed (for example with an octopus type mechanical element) to remove the most bulky materials, such as furniture type material , mattresses, etc., which can block the separators of the following stages, a step (b) of separation and classification by means of a screen having openings of approximately 60 mm to approximately 100 mm to form a first through current and a first current of sinking in which the first through current is enriched in rolling stock and combustible material compared to the first sinking current; in a third stage (c) the first through current is separated by at least a sieve of openings between 170 mm and 380 mm in a second sinking current and a second through current, the second sinking current of stage (c) it is separated and classified in a stage (d) of classification to form:
- a stream of fine material with a diameter smaller than the same opening as the screen described in step (b) comprising fermentable material enriched in organic matter,
- a stream of rolling stock is enriched in plastic and - a stream comprising the planar material, such as paper, cardboard, plastic film, sheets, textiles, among others;


in step (m) the metals present in the rolling stock stream are recoveredfrom step (d) obtaining a metal-free recyclable current;in stages (e) and (f) the metallic materials (ferric and non-ferrous) are recovered,respectively) of the first sink current;
5 in step (g) the current obtained after stages (e) and (f) is passed through a sieve of openings of 5 to 20 mm to form the third sinking current and a third through current; in step (h) a densimetric separation of the third through current is carried out to form a first light current and a first dense current;
10 in step (i) the first light stream is separated by a screen of openings between 25 and 50 mm to form a fourth sinking current and a fourth through current; in step (j) the recyclable plastic materials of the fourth through current are recovered by means of an optical separator to form a current;
15 in step (k) heavy inerts such as stones, sand, glass, etc. are eliminated. of the fourth sinking current to form a current which, after joining the rejection current obtained from step (j) and undergoing a size reduction process (l forms the clean bio-waste stream (step (l)); in stage (n) the bio-waste stream of stage (l) is mixed with a solution
20 of water, acid or base to form the stream of impregnated bio-waste; in step (o) it is subjected to the stream of bio-wastes impregnated from stage (n) at a temperature of between 100 ° C and 250 ° C and at a pressure of between 100 kPa and 4,000 kPa for a time of between 1 and 20 minutes; and subsequently the pressure is reduced to less than 35 kPa to form the pretreated bio-waste stream;
In step (p), water, acid or base is added to the pretreated bio-waste stream of step (o) to form a pre-treated bio-waste suspension with suitable pH and humidity conditions for the following stages; and in step (q) the pretreated bio-residue suspension of step (p) is contacted with an enzymatic cocktail comprising at least one cellulase enzyme
30 so that after a residence time an enzyme hydrolyzate suspension comprising monosaccharide sugars and non-fermentable material is formed, where the non-fermentable material is removed from the enzyme hydrolyzate suspension during or after enzymatic hydrolysis at an optional stage (r), resulting in a stream rich in fermentable material.


The stream rich in fermentable material obtained in stage (r) can be returned to stage (q) to reduce the viscosity of the mixture and increase the residence time of the fermentable material in that stage or be taken directly to a next stage
(y) that it can be a fermentation with yeasts or alternatively a concentration stage to produce a sugar syrup.
In a preferred embodiment of the process of the invention, glass is recovered in an optical separator from the third sinking current of step (g) and / or the current comprising removed materials, after recovering non-ferrous materials, in the stage (k).
In another preferred embodiment of the process of the invention, the second through current obtained in step (c) is carried, after passing through a manual triage to a density separation stage where a second light current and a second rich dense current are obtained. in non-combustible inert matter such as stones, metals, etc. The second light stream enriched in combustible material is crushed at a later stage to give rise to a stream called solid recovered fuel (CSR) derived from waste and with high calorific value.
In another preferred embodiment of the process of the invention, the enzymatic cocktail of step (q) further comprises at least one hemicellulase.
In another preferred embodiment of the process of the invention, in step (n) the bio-waste stream is impregnated with acid and in step (p) an aqueous solution of ammonia is added, resulting in a pre-treated bio-residue with a solids content of between 15-30% by weight, a pH between 4 and 6 and a temperature between 30ºC and 70ºC. Preferably the acid is an inorganic acid and the pretreated bio-waste stream comprises 0.01 to 0.15 kg of acid per kg of dry-based bio-residue.
In another preferred embodiment of the process of the invention, in step (n) the bio-waste stream is impregnated with base and in step (p) an acid solution is added, resulting in a pre-treated bio-residue with a solids content of between 20% and 30% by weight, a pH between 4 and 6 and a temperature between 30 ° C and


70 ° C Preferably the base is ammonia and the pretreated bio-waste stream comprises 0.1 to 2.5 kg of ammonia per kg of solid-base bio-residue.
In another preferred embodiment of the process of the invention, the current of5 Enzymatic hydrolyzate suspension of step (q), during or after thehydrolysis, dehydrates to form:
-  a second stream of impregnated bio-waste having a solids content of between 30% and 70% by weight, preferably between 40% and 60% by weight; Y
-  an aqueous stream that is a stream rich in fermentable material;
10 where at least a part of the aqueous stream can be returned to stage (q) or sent to the fermentation stage (y).
In a preferred embodiment, the above aqueous stream is concentrated to form a syrup rich in monosaccharide sugars with a content of monosaccharide sugars
15 of more than 25% of total sugars.
In another preferred embodiment, a yeast is added to the above aqueous stream or to the enzyme hydrolyzate suspension stream of step (q) to transform the sugars into an organic compound selected from alcohols,
20 preferably ethanol, or organic acids. Preferably the yeast is Saccharomyces cerevisiae.
In another preferred embodiment of the process of the invention, in step (o) the stream of bio-waste impregnated from step (n) is contacted
25 with steam at a temperature between 130 ° C and 250 ° C, preferably between 150 ° C and 220 ° C, and at a pressure between 400 kPa and 1,570 kPa, preferably between 625 KPa and 1,450 kPa, for a time between 1 minute to 5 minutes; Y
-  the pressure is reduced to a value between 1 to 35 kPa in a single stage; or
-  the pressure is reduced to a pressure of between 40% to 60% in a first stage
30 pressure reduction; said pressure is maintained for a period of time between 0.5 minutes to 20 minutes and subsequently the pressure is reduced to between 1 to 35 kPa in a second pressure reduction stage.
BRIEF DESCRIPTION OF THE DRAWINGS


Fig. 1 is a block diagram of the separation and classification stages of the gross MSW and the cleaning and treatment of the bio-waste stream. DETAILED DESCRIPTION OF THE INVENTION
In the present invention there is provided an integrated process for preparing monosaccharides from a classified mixture of solid waste by enzymatic hydrolysis of classified (fractionated) bio-waste streams enriched in cellulosic components and comprising non-component components.
10 fermentable. In accordance with the present invention, a certain part of the non-fermentable material present in the solid waste mixture is carried out in an enzymatic hydrolysis in aqueous medium from which it is removed from the process with methods of classification by wet route. The elimination of a certain part of the non-fermentable material in a stage of fractionation in aqueous medium together with the stage of
Enzymatic hydrolysis allows for improved elimination efficiency compared to a dry solid sorting stage, and allows the combination of the non-fermentable material classification and enzymatic hydrolysis stages thus providing greater efficiency of the sorting process. and higher performance due to the elimination of the need for one or more stages of
20 classification of non-fermentable material by dry route that are otherwise necessary to eliminate the non-fermentable fraction.
The sorting steps (selection or separation) by dry route of the present invention to perform the fractionation of the solid waste mixture to form a stream of bio-wastes enriched in cellulosic compounds for conversion into monosaccharides comprises combinations of fractionation techniques that include , but not limited to, manual separation, separation according to the size of the material, separation according to the density of the material, separation according to the dimension of the material, separation according to the optical properties of the material, and separation according to X-ray absorption properties of the material . A certain portion of the non-fermentable material is sent to enzymatic hydrolysis together with the cellulose-rich bio-residue, where it is removed from the hydrolyzate during or after hydrolysis. Said non-fermentable material includes, for example, wire, plastic, rope, sand, brick, metal objects, cork and the like. The wet classification steps of the present invention include without limitation, at least


one between flotation, foam removal, filtration, screening, hydraulic classification, rakes, removal of dense solids from a gutter of residual material that comes out of the bottom of the enzymatic hydrolysis vessel, clamps, waste flaps, and extraction tubes. The complete processes of classification of the mixture of solid waste and generation of monosaccharides provide an efficient generation of several high-value recovered streams including sugar streams comprising glucose, xylose, and their combinations and recovered streams for recycling and reuse including classified plastics, paper, cardboard, beverage cartons, glass and / or metals. In the present invention, plastics streams are also provided which can be classified according to the type of plastic, such as polyethylene terephthalate ("PET"), high density polyethylene ("HDPE") and polyvinyl chloride (" PVC "). Additionally, the present invention also provides a solid recovered fuel ("CSR") that is suitable for use as a power source in steam generation boilers and cement production furnaces. The present invention further provides the recovery of paper and cardboard suitable for sale as recyclable material.
As used in the present invention, solid waste mixture refers to a waste stream comprising bio-waste (for example, food waste and garden waste), inorganic materials (eg, dirt, stones and debris), mixing of plastics (for example, at least PET and HDPE), metals (for example, iron, steel, aluminum, brass and / or copper), fiber (for example, paper and cardboard ("P&C")), glass, textiles, rubber and wood An example of solid waste mix is the MSW.
As used in the present invention, RSU refers to the solid waste mixture stream predominantly comprising a mixture of urban and commercial waste. Although the precise composition of the MSW varies with the source, and the concentrations and intervals disclosed in this paragraph should not be taken as a limitation, the MSW typically include, without limitation, the components detailed in the following Table 1 (on a wet basis). ):
Table 1
Component RSU 1RSU 2RSU 3
Organic fraction 30% to 80%35% to 75%40% to 70%


Food waste 5% to 55%10% to 50%15% to 45%
Garden waste 2% to 25%3% to 20%5% to 15%
Metals 0.1% to 10%0.5% to 5%1% to 3%
Plastic 3% to 30%5% to 25%10% to 20%
PET 0.1% to 5%0.5% to 3%1% to 2%
HDPE 0.1% to 5%0.3% to 3%0.5% to 1.5%
Glass 1% to 10%2% to 8%3% to 6%
Rubber, leather, textiles 1% to 20%3% to 15%6% to 11%
Inorganic material 0.1% to 20%0.5% to 15%1% to 12%
Combustible material (for example, wood, paper) 5% to 35%10% to 30%15% to 25%
The mixture of solid waste and MSW can be further characterized as a mixture of (i) planar material (or two-dimensional components) such as paper, cardboard, plastic film and at least part of the mixture of metal components and (ii)
5 rolling stock (or three-dimensional objects) such as bottles, cans, beverage cartons, inorganic material, glass, at least a part of the mixture of metal components, and a predominant part of the organic fraction.
As used in the present invention, "bio-waste" refers to a stream
Fractional enriched in organic material suitable for conversion to monosaccharides such as, for example, glucose and / or xylose. The organic material includes, but is not limited to, starch, cellulose, lignocellulose and hemicellulose. Bio-wastes are characterized by comprising at least 30% by weight, at least 35% by weight, at least 40% by weight, at least 45% by weight, at least one
15 50% by weight, at least 55% by weight, at least 60% by weight, at least 65% by weight, at least 70% by weight, at least 75% by weight, at least 80 % by weight or at least 85% organic material (ie "organic content"), and its ranges, such as from about 50 to about 85% by weight, or from about 60 to about 80% by weight of
20 organic material.
As used in the present invention, "predominant", "predominantly comprising" and "substantial" are defined as at least 50%, at least 75%, at least 90%, at least 95% or at least 99% as p / p%, p / v% or


v / v%.
As used in the present invention, "recyclable material or M.R." refers to the components of the waste mix that have value and include, but are not limited to, paper, cardboard, metals, glass, beverage cartons, plastic, and combinations thereof.
As used in the present invention, "enriched" refers to a fractional process stream or a fractionated constituent that has a concentration of a cited component that is greater than the concentration of said component (i) in the
10 process stream or a constituent from which the fractional process stream or fractionated constituent is produced or (ii) in one or more streams simultaneously divided or one or more components divided simultaneously.
In the present invention, the solid waste mixture can be pre-classified manually, and / or any of the different fractional waste streams can be further processed by manual sorting to recover dangerous objects and materials, remove objects that could damage the sorting equipment of RSU and / or recover objects that are large and have a high recovery value.
20 Manual sorting can be carried out by personnel on one or more preclassification lines such as placing the waste on a sorting conveyor belt in which the preclassified objects are identified and removed. Examples of manually classified objects include electronic waste, structural steel, tires and tires, containers comprising pressurized compounds (e.g.,
25 propane), concrete blocks, large rocks, pallets, cardboard, plaster, and the like. In addition, hazardous waste such as solvent and chemical containers, paint cans and batteries are preferably removed before fractionation to avoid contamination of bio-waste and other materials from the waste mixture.
In the present invention, the solid waste mixture, optionally subjected to a manual preclassification stage, is classified in a first stage of fractionation by stepwise separation (eg screening) to form at least two sized residual currents comprising a first
35 sink current (4) enriched in organic fiber compared to the mixture


of solid waste and a first through current (5) enriched in rolling stock and combustible material (eg paper and cardboard) compared to the solid waste mixture. The sieve opening size can be between 60 mm and 100 mm, preferably between 70 mm and 90 mm, and more preferably of
5 approximately 80 mm. Suitable screening devices include rotary trommels, disc sieves, vibrating sieves and oscillating sieves, among others known to any person skilled in the art.
In the present invention, rotary trommel type sieves are used. A sieve type
The rotary trommel normally comprises a perforated cylindrical drum or a cylindrical frame that holds a perforated sieve. The trombone can be adequately raised at an angle at the end of the feed or at the end of the discharge, or it may be not raised (i.e. flat). Size separation is achieved as the fed material spirals or otherwise as it progresses
15 inside the rotating drum / sieve, where the material of smaller size than the sieve openings passes through the sieve as a sinking fraction and the material larger than the sieve openings is retained if it moves forward as a fraction intern For the drum component, an internal screw can optionally be used when the drum arrangement is flat or raised
20 at an angle less than about 5 °. The internal screw facilitates the movement of the objects inside the drum, forcing them into a spiral movement. Any of the different trommel designs known in the art is suitable for practicing the different embodiments of the present invention. For example, you can use a trombone that has two or more concentric sieves with the
25 thicker sieve located in the innermost section. Alternatively, the tromers can be arranged in series so that the screened and / or retained material exiting a first trommel can be subsequently fed to a second trombone or a series of trommels. Still alternatively, a trombone having at least two sections with different aperture sizes can be used, being optionally arranged
30 said series trombone with one or more additional trommels as described above. The sieve type can take different configurations. The sieves can be suitably perforated plates or mesh sieves where the openings can have both square and round shapes.
35 Screen optimization can be based on one or more of the following variables: (i)


the necessary dimension of the sieved product, (ii) the opening surface where a square opening provides a surface greater than a round opening having the same diameter as the length of the square opening, (iii) the degree of agitation of the material, ( iv) the speed of rotation of the trommel, (v) speed of
5 feeding, (vi) residence time of the material, (vii) angle of inclination of the drum, (viii) number and size of sieve openings, and (ix) characteristics of the feeding.
Typically, a size fractionation stage is associated with a size of
10 cut where fractional particles are characterized by a distribution of the particles. In the case of size fractionation, the distribution often includes a number of particles or objects above or below a particular cut, such as a sieve having a fixed aperture size, such as 10 mm, 25 mm, 60 mm , 80 mm or 100 mm. Unless otherwise specified, a number of
Cutting (for example, 80 mm) generally means that at least 75% by weight, at least 80% by weight, at least 85% by weight, at least 95% by weight, at least 95 % by weight or at least 99% by weight of the particles or components have a size greater than the cut-off number (in the case of the through current) and at least 75% by weight, at least 80% by weight , at least
20 85% by weight or at least 90% by weight, at least 95% by weight or at least 99% by weight of the particles or components are smaller than the cutting number (in the case of sinking current). In other words, an average particle size refers to a particle size distribution where at least 75% by weight, at least 80% by weight, at least 85% by weight or at
25 minus 90% by weight, at least 95% by weight or at least 99% by weight of the particles or components pass through a sieve having a specific aperture size. In another characterization of fractionation by size, the dimensioned residual currents have a size distribution with a ratio between small particles and large particles, that is, the relationship between
30 particles above the cut and particles below the cut, less than 25, less than 20, less than 15, less than 10, less than 8, less than 6, or less than 4. In the case of fractionation by density or spatial configuration (shape), the distribution often includes a particle number or objects above or below a particular cut, that is, density or shape (two-dimensional or
35 three-dimensional). Unless otherwise specified, a density cut number


means that at least 50% by weight, at least 60% by weight, at least 70% by weight, at least 80% by weight or at least 90% by weight, such as about 60% by weight at about 90% by weight or from about 60% by weight to about 75% by weight, the particles or 5 components have a density greater than the cut-off number (in the case of a passing current) and at least 50% by weight, at least 60% by weight, at least 70% by weight, at least 80% by weight or at least 90% by weight, such as from about 60% by weight to about 90% by weight or from about 60% by weight to about 75% by weight, of the particles or
10 components have a density less than the cutting number (in the case of a sinking current).
In the present invention, the screen of the first stage of fractionation by size can be used in series with at least a second stage of fractionation by size to form at least three sized fractional residual currents. The second sieve opening in the second size fractionation stage is between 170 mm and 380 mm, preferably between 200 mm and 350 mm. In said steps, a current of size between approximately 80 and 200 mm is obtained, a second current of size between approximately 200 and 350 mm and a third current of size greater than approximately 350 mm. All of them are enriched in recyclable material with respect to the current that feeds the first stage of separation. The current of between 200 and 350 mm will be enriched in recyclable material, while the current greater than 350 mm will contain large recyclable material, and will be rich in material
25 fuel
In the present invention, magnetic separation devices can be used at different points of the systems of the present invention to collect ferrous metals. Examples of magnetic separators include magnetic drum, tape
30 magnetic perpendicular, heads with magnetic pulleys, and the like. Suitable locations include, without limitation, retaining and sieving outputs of the trommel and transport systems, heavy current outputs of density separation and transport systems, enzymatic hydrolysis vessel, and optical classification and ray classification systems X.


In the present invention, one or more electrostatic separators for the isolation and separation of plastic components can be operated together with one or more of the systems described in the present invention, including air fractionation, fractionation sieves, transport systems and transfer of material, 5 optical and X-ray classifiers, and bag opening devices. Electrostatic separation systems are known in the art and are commercially available. The separation of plastics according to the type can be carried out by electrostatic separation where a current comprising a mixture of plastics is electrostatically charged (for example by friction or application of a charge) 10 resulting in a positively and negatively charged material, where PE, PVC and PET plastics have a characteristic and different induced load. For example, PE and PET normally assume a positive charge and PVC normally assumes a negative charge. Positively and negatively charged materials are passed through an electrostatic field formed by counter electrodes on sides
Opposites where the positively charged plastic migrates to the side of the negative electrode and the negatively charged plastic migrates to the side of the positive electrode resulting in separation of the plastic by type. The manufacturers of electrostatic separation systems include Hitachi Zosen Corporation and others.
The first through current (5) is classified, as already described, to separate a stream of fine material (8) (rich in organic matter), a stream of planar material (10) composed of two-dimensional material (eg paper and cardboard ("P&C")) and a stream of three-dimensional rolling stock (9) composed of plastics.
In an embodiment of the present invention, the second sinking current (6) of between 200 and 350 mm approximately, enriched in recyclable material is processed in a bag opening apparatus to release the components that may still be included therein. before additional classification.
The classification of any of the different process streams comprising a mixture of two-dimensional (planar) and three-dimensional (rolling) objects, such as the second sinking current (6) enriched in material and / or the currents comprising a component which has a density higher than the rest of
35 components, can be achieved by any of the different techniques of


density separation known in the art such as ballistic separators or air separators (for example, linear air separators (windshifters pneumatic separators) and rotary air separators).
In the present invention, the classification of the currents enriched in recyclable material is carried out by ballistic separation with screening. In general, ballistic separation with screening separates feed streams based on their size, density and shape properties to form a first fraction comprising rolling objects (e.g., containers, plastic bottles, stone,
10 boats and some metal objects), a second fraction comprising flat materials (planar) and light materials (for example sheets, textiles, paper and cardboard), and a third fraction of fine material (for example, organic material, food and sand ). Such ballistic separators generally comprise an upward slope ramp from the end of the feed to the end of
15 discharge and additionally include a perforated conveyor. As the material is transported, the rolling material rotates in the direction of the point of least elevation at the end of the feed and forms the fraction of rolling stock, the fine material elements pass through the sieve and constitute the fraction of fine material, and the light and flat density elements are transported to the exit for
20 form the stream of flat material. Optionally, air can be blown from the feed end to the discharge end to improve the separation efficiency of the flat material and the rolling stock, and the conveyor can be vibrated or oscillated, optionally, to improve efficiency of separation of fine material.
The optimization of ballistic separation can be based on one or more of the following variables: (i) the desired particle size of the fine material, (ii) the location of the feed on the conveyor belt, (iii) the speed of feeding, (iv) the residence time of the material, (v) the angle of inclination of the
30 conveyor belt, (vi) the number and size of the sieve opening, (vii) the feeding characteristics, (viii) the air velocity and (ix) the degree of vibration or oscillation.
In the present invention, the opening of the perforations of the ballistic sieve 35 (openings) may suitably be between 60 mm and 100 mm. Openings


They can have both square and round shapes. In the present invention, the size of the opening can be approximately 80 mm. The fine material is an 80 mm through stream characterized by having an organic content of at least 40% by weight, at least 50% by weight or at least 60% by weight. The recyclable material stream is characterized by a glass component and a mixed plastic component comprising PET and HDPE. The recyclable material may further comprise a mixture of metals including aluminum, brass, copper, iron and steel. The planar material stream is characterized by a P&C component. The planar material stream is further characterized by having a component
10 fuel that has a calorific value of at least about 15, 16 or 17 megajoules per kilogram on a dry basis (approximately 7,500 Btu per pound).
Any of the different streams rich in combustible material (24), (25) or (34) within the scope of the present invention can optionally be conditioned to form CSR. According to the present invention, several streams rich in combustible material can be combined and processed. The material is separated by air classification as described in the present invention (such as a linear air separator) to form a second light stream (26) and a second heavy or dense stream (27) in which the light stream is enriched in combustible material 20 compared to heavy current. The light stream is processed in a crushing step to further reduce the volume and particle size and form the CSR. A typical average particle size is about 20 mm to about 50 mm. The CSR can be optionally dried to increase the energy value per unit of weight. The CSR is characterized by having a calorific value of between about 17 and about 30 megajoules per kilogram (from about 7,500 to about 13,000 Btu / lb) and less than about 25% by weight of water. The CSR within the scope of the present invention can be suitably used as an energy source for boilers and cement kilns,
30 or as a gasification substrate.
The paper and cardboard stream (38) obtained from the planar stream (10) can optionally be crushed before, after or in parallel mixing with the aqueous solution (36).


The paper and cardboard stream (38) can be suitably combined with the clean bio-waste stream (20) generated from the stream enriched in organic matter in the first stage of size fractionation and the combination of streams can be subjected to impregnation with acid and pretreatment. This
Pretreated stream (29), which comprises monosaccharides and soluble polysaccharides, can be contacted with a source of enzymes comprising cellulase.
The fractional recyclable material stream can be further classified by any optical classification system, X-ray separation, and its 10 combinations to fractionate the recyclable material stream into a different streams of plastic material. In the present invention, the recyclable material stream can be fractionated by optical classification and / or X-ray separation as described in the present invention, optionally with the additional combination of at least one manual classification stage, to isolate a number of 15 streams rich in recyclable materials including a plastic film stream, a HDPE stream, a PET stream, and a stream of mixed plastics. Other possible streams rich in recyclable materials generated from the fractional rolling stock stream may include, a stream of PVC plastic, a mixed metal stream, metal streams classified by alloy (eg, aluminum, brass and copper), a stream of beverage cartons, a stream of paper and / or a stream of cardboard. The residual material remaining after the fractionation of the rolling stock stream is enriched in combustible material compared to the different recovered streams, and can be sent to the CSR formation conditioner as described herein.
Invention
In the present invention, the planar material stream generated in the two-dimensional / three-dimensional fractionation (eg ballistic separation) of the stream enriched in recyclable material generated in the first stage of size fractionation can optionally be classified by optical classification as described in the present invention to form a recovered P&C stream (38) and a stream rich in combustible material (34). The stream rich in combustible material is sent to the conditioner as described in the present invention. The P&C current can have value as recovered current or it can be processed by cleaning bio-waste and grinding as described in


the present invention to form cellulosic material for conversion into monosaccharides.
Optical classifiers are known in the art and include, but are not limited to,
5 near infrared (NIR) and color camera classifiers. For example, in one embodiment, the optical classifier can be operated by scanning the intermediate waste stream in free fall by means of a camera sensor. Other optical classifiers use near infrared and other scanning technologies to separate the desired materials from mixed currents. In the present invention, the
10 streams of mixed plastics can be classified by the type of plastic based on the reaction principle of electrons in the material of the objects to be analyzed under infrared light, where the molecules in the object to be analyzed react with infrared light with a model of electronic excitation characteristic of the composition of the material. The infrared detector and the associated computer read and interpret the model,
15 assign a type of material (for example, HDPE, PET or PVC plastic) according to the interpretation, and classify (separate) objects based on the type of material. A sensor (such as a camera or light sensor) detects a characteristic signal of the material to be separated and transmits the detection signals to a computer system where the signals are analyzed by executing an algorithm in the computer system to
20 determine the relative composition or identify the material with respect to a pre-configured composition or relative value. The computer system transmits an output signal to activate air jets to quickly eject the material while it is in free fall. Any number of optical classifiers can be used in series or in parallel. The manufacturers of optical classifiers include TiTech
25 Pellenc, MSS, NRT, and others.
X-ray classification systems are based on the measurement of X-ray absorptions in a material at different energy levels in order to determine the relative atomic density of the material. More particularly, the absorption of X-rays in a material is a function of the atomic density of the material and also a function of the energy of the incident X-rays where a given piece of material will absorb X-rays to varying degrees depending on the energy of the materials. incident x-rays An X-ray sensor detects a characteristic signal of the material to be separated and transmits the detection signals to a computer system where the 35 signals are analyzed and an algorithm is executed in the computer system to


determine the relative composition or identify the material with respect to a pre-configured composition or relative value. The computer system transmits an output signal to activate air jets to quickly eject the material while it is in free fall. This technology can evaluate the entire object and examine the
5 complete object taking into account exterior and interior variations. Such classification systems are described in U.S. Patent No. 7,564,943 and are commercially available, such as from National Recovery Technologies, LLC of Nashville, Tenn. X-ray classifiers can be used in combination with optical classifiers.
In some classification systems by X-ray absorption, a matrix of dual-energy X-ray detectors is placed below the surface of a conveyor belt used to transport mixed waste through a detection region located between a matrix of detectors and an x-ray tube.
15 suitable detector arrays can be obtained from Elekon Industries (Torrance, Calif.) And X-ray tubes can be obtained from Lohmann X-ray (Leverkusan, Germany). The X-ray tube is preferably a broadband source that radiates a sheet of X-rays preferably collided through the width of the conveyor belt along the array of dual-energy X-ray detectors
20 so that X-rays pass through this detector region and the conveyor belt before reaching the detectors. As the material passes through the X-ray detection region, the X-rays transmitted through it are detected by the array of dual-energy X-ray detectors at two different energy levels. The detection signals are transmitted to a computer system and the signals are
25 analyze by executing an algorithm in the computer system to determine the relative composition of the material with respect to a preconfigured relative composition. A matrix of high-speed air ejectors is arranged downstream with respect to the detection region and is located across the path width of the materials discharged by the end of the conveyor belt. The computer
30 executes the algorithm of classification and selection of materials and, according to the results derived from the execution of the algorithm, the signals of the computer system select which air ejectors from the matrix of air ejectors will be activated and expelled, in this way , the materials selected from the material flow according to the calculated relative composition. The sequence of detection, selection, and
The ejection can take place simultaneously on multiple paths along the


width of the conveyor belt so that multiple samples of material can be analyzed and sorted at the same time.
Optical classification systems and X-ray classification systems
5 can be configured to scan a stream of a waste mix and determine if the material to be analyzed is a particular type of material such as plastic, paper, or glass, and recover (i) HDPE plastic, PET plastic, plastics No. 3 to 7 and / or plastics of the polyvinyl chloride (PVC) type, (ii) glass and / or (iii) paper of a stream of a mixture of residues comprising organic particles and / or
10 inorganic particles. Optical sorting systems and X-ray sorting systems can be further configured to distinguish between types of plastics, such as HDPE plastic, PET plastic and PVC plastic so that a stream containing a mixture of plastics can be classified into streams According to the type of plastic. For example, after the detection of a material
Particularly in a stream of a waste mixture, an optical classification system or an X-ray classification system, it can use air directed towards the nozzles to expel the searched / identified material to produce one or more recycled products such as recyclable PET, HDPE recyclable, recyclable plastic film, recyclable plastics No. 3 to 7, recyclable glass and / or paper products
20 recyclable
More particularly, for example, a waste mixture can be introduced into a conveyor, the speed of which is selected such that the waste mixture is released by the end of the conveyor. The optical sensor or the X-ray system is programmed by a computer program in a computer system to detect the shape, type of material, color or translucency levels of particular objects. For example, the computer system connected to the optical sensor or X-ray system can be programmed to detect the type of plastic material associated with plastic bottles, such as PET, HDPE, and PVC. Objects that have the characteristics of preprogrammed material are detected by the optical sensors or the X-ray system when they pass through a beam of light or X-ray and the computer system connected to the sensor sends a signal that activates an ejection air nozzle at high pressure The ejection air nozzle releases a stream of air that strikes the detected objects downwards to remove them from their normal trajectory in the direction of a first hopper and / or a first conveyor. Other materials and objects


They continue their movement along the path to a second hopper and / or a second conveyor. In the present invention, any of the different currents generated in a first optical / X-ray classification system can optionally be processed in at least one optical / X-ray classification system.
5 to produce any one of a stream of plastic material classified by type (for example, PET, HDPE or PVC), glass classified by color, paper. The rest of the residual current from one or more optical / X-ray classifiers is typically a stream of particulate material rich in organic material.
In any of the various embodiments, the first sunken stream (4) from the first stage of fractionation enriched in bio-waste can be fractionated with a sieve and having a mesh size of about 5 mm to about 20 mm, of about 5 mm to approximately 15 mm, from approximately 8 mm to approximately 12 mm, or approximately 10
15 mm to form (i) a third sunken stream (13) enriched in inert compounds and having an inorganic material content of at least 50% by weight, at least 55% by weight, or at least 60% in weight, such as about 65% by weight and an organic matter content of less than 50% by weight, less than 45% by weight or less than 40% by weight, such as about 35%
20 by weight and (ii) a third through current (12) of crude bio-waste having an organic matter content of at least 40% by weight, at least 45% by weight, at least 50% by weight, at least 55% by weight or at least 60% by weight and having an average particle size between about 5 mm and about 80 mm, between about 10 mm and about 80 mm,
25 or between about 15 mm and about 80 mm, and further comprising a recyclable component comprising plastic. The third sinking stream (13) rich in inorganic material can be optionally purged from the process or can be further processed for glass recovery.
In the process of the invention, any of the different bio-waste streams can be processed by density separation to form a first or second dense rejection stream and an intermediate bio-waste stream. The first dense rejection stream (15) is enriched in inorganic compounds, glass and metal, and has a high density in grams per
35 cubic centimeter, compared to the intermediate bio-waste stream. The


The first rejection stream can be purged from the process, or it can be further processed for the recovery of the metal and / or glass included by any of the different methods described in the present invention. Suitable density separation methods are known in the art and include, without limitation, air separators such as linear air separators (available, for example, from Nihot) and rotary air separators. Linear air separators separate a feed stream into light and heavy fractions where light materials are separated from heavy materials in a separation unit with air flow control. The light materials are separated from the air stream 10 in the separation unit and transported outside the separation unit and the heavy fraction is sunk into the separation unit. The separation efficiency varies with the composition of the feed stream, but typically from about 70% by weight to about 80% by weight or from about 75% by weight to about 85% by weight of the inert material 15 (eg, material inorganic) is separated from the heavy fraction and at least 95% by weight or at least 98% by weight of the paper and the cardboard is separated from the light fraction. The rotary air separation comprises a device that has an opening provided with a sleeve through which air is blown, the sleeve being surrounded by a cylindrical sieve that rotates past the opening. The material to be separated is deposited on the sieve in the area of the sleeve opening, and the fine material is drawn through the sieve as a through stream (as allowed by the size of the sieve opening) and transported by the air flow to a first collection point. The retained material is dragged into the sieve and transported through the sieve beyond the sleeve to the opposite side of the separator where it is collected in a second
25 collection point. Dense sieved material (such as gravel) is not transported through the sieve, but instead falls from the sieve on the side of the equipment feed and is collected at a third point.
The first light stream (14) is a stream enriched in bio-waste in which
The light stream can be fractionated with a sieve as described in the present invention and having a mesh size between 25 mm and 50 mm to form (i) a fourth stream of sunken bio-waste (16) having a content in organic matter of at least 65% by weight, at least 70% by weight, at least 75% by weight or at least 80% by weight, such as about 70% by weight
35 weight at about 85% by weight or about 70% by weight at


about 80% by weight and having an average particle size of less than about 50 mm, and (ii) a fourth pass-through current (17) that comprises organic material and is enriched in recyclable material (eg paper, cardboard, plastic , and their combinations) compared to the current of
5 sunken bio-waste.
In the process of the invention, the fourth through current (17) can be classified by optical classification and / or classification by X-rays to recover a stream enriched in bio-waste (19) and a recovered current
10 enriched in plastic (18), compared to the fourth through current (17).
In the fourth sinking current (16) they are fractionated by optical or X-ray classification. In a preferred embodiment of the present invention, the classification by X-rays is used. With this classification system inerts are eliminated
15 of the fourth sinking current (16). This inert current, mainly composed of stones, bones, sands, is eliminated from the process.
The clean stream joins the stream rich in organic matter (19) and is subjected to a grinding process to reduce its size to an average of
20 particle less than approximately 25 mm.
The bio-waste stream (20) is the clean bio-waste stream for conversion into monosaccharides. Said stream has an organic material content of about 70% by weight to about 90% by weight or of
25 about 75% by weight to about 90% by weight.
In another stage of the fractionation of the crude bio-residue of the present invention, the fourth through current (17) enriched in recyclable material is further fractionated by optical classification to recover streams comprising a stream rich in organic material (19) and a stream of recirculation that is enriched in recyclable material (18). In any of the embodiments of the process of the invention, paper, cardboard, glass, metals and / or plastics can be recovered as individual fractions or streams. The stream rich in organic material is characterized by a particle size greater than 50 mm, and more preferably between 50 mm and 80 mm. This current is preferably


grinding as described in the present invention to reduce the particle size to less than 25 mm. The ground stream or not is then combined with the clean bio-waste stream for conversion into monosaccharides. The stream enriched in recyclable material (18), or its individual fractions, are transported
5 to the fractionation of recyclable material by ballistic separation by optical classification and manual classification as described in the present invention for the recovery or purification of plastics, metals, glass, paper and cardboard.
At another stage of the fractionation of the crude bio-residue of the present invention, (i) the crude bio-waste stream can be fractionated with a sieve having a mesh size between 5 mm and 20 mm, more preferably about 5 mm at approximately 15 mm to form a third sunken stream (13) and a third current of bio-waste (12) and (ii) the third stream of bio-waste
Through-pass (12) is fractionated by density separation to form a first dense rejection stream (15) and a first stream of light bio-waste (14) as described in the present invention. The first stream of light bio-waste is fractionated with a sieve having a mesh size between 25 mm and 50 mm, to form a fourth sinking current and a fourth through current in which
20 the fourth through current (17) is enriched in recyclable material compared to the fourth sunken stream (16) and the fourth sunken stream (16) is enriched in bio-waste compared to the fourth through current (17). The fourth through current is fractionated by optical classification and / or X-ray classification to recover a current rich in recyclable material to
25 from the fourth intern current. The recycled product streams comprising plastics, metals, glass, paper and / or cardboard can be generated by optical classification and / or X-ray classification, or the fourth clean through current (or fractions thereof) can be transported up to the fractionation of rolling stock by ballistic separation by optical classification and manual classification
30 as described in the present invention for the recovery or purification of plastics, metals, glass, paper and cardboard.
Rejection currents from the bio-waste cleaning area; Third sinking current (13) and current (22) can be sent to a glass recovery area. These rejection streams, which may have a content in


Glass between 10 and 40% by weight or between 20 and 50%, are divided by an optical separator or an X-ray separator generating at least two currents. At this stage at least two currents are generated, one of them enriched in one or more types of glass when compared to the feed current and the other output current, 5 having a glass content of between 50 and 99% in weight, preferably between 60 and 90% or between 70 and 80%. The rejection fractions from the bio-waste cleaning area, enriched in one or more types of glass are subjected to a preconditioning stage to the optical or X-ray separator. This conditioning stage may comprise a density separation, a
10 separation by size or a combination of both, thus increasing the percentage of glass entering the recovery stage.
In this invention, the streams enriched in bio-waste generated from the sunken stream of the first fractionation stage and having an average particle size greater than about 25 mm are preferably ground to reduce the particle size to less than about 25 mm to maximize the relationship between the surface area and the weight ratio to increase the efficiency of glucose hydrolysis. Any suitable grinding device, such as a chopper, hammer mill, crusher, mill, can be used
20 of blades, cutter, disc mill, centrifugal mill or homogenizer. The recovered milled bio-waste stream is combined with the clean bio-waste stream and subsequently converted to glucose by hydrolysis.
The clean bio-waste stream may comprise a content in matter
Organic of between 70% by weight to 90% by weight or preferably from 75% by weight to 90% by weight. The clean bio-waste stream comprises a soluble organic component and an insoluble organic component. The soluble organic component comprises from about 2% by weight to about 10% by weight of glucan, more preferably from about 2% by weight at
About 5% by weight of glucan and from about 0.05% by weight to about 1% by weight of xylan. The insoluble component comprises from about 5% by weight to about 20% by weight of glucan, preferably from about 8% by weight to about 20% by weight of glucan, from about 1% by weight to about 10% by weight of
35 xylan, preferably from about 2% by weight to about 5% in


Xylan weight, from about 20% by weight to about 40% by weight of cellulose, preferably from about 25% by weight to about 35% by weight of cellulose and from about 5% by weight to about 15% by weight of lignocellulose. The clean bio-waste stream also includes
5 less than about 40% by weight of ash (inorganic materials), preferably less than about 35% by weight, 30% by weight, 25% by weight, 20% by weight or less than about 15% by weight of ash (inorganic materials).
10 The clean bio-waste stream generated from the sunken current of the first fractionation stage, the stream of fine material enriched in organic matter and / or stream of planar material (paper and cardboard) generated from the through-stream of the first stage of fractionation, collectively referred to as "clean bio-waste", can be converted by one or more stages
15 of hydrolysis to achieve an aqueous stream comprising glucose. The glucose stream can be purified to remove impurities and C5 monosaccharides (eg, xylose). In further embodiments of the present invention, the glucose stream can be contacted with a source of at least one fermentation organism to form a fermentation product.
In the conversion of bio-waste of the present invention, the clean bio-waste is combined, or impregnated, with at least one aqueous stream with stirring to form a clean bio-waste suspension having a water content of between 50 to 90%. by weight, preferably between 60 to 80% by weight,
25 more preferably from 70 to 80% by weight.
The clean bio-residue can be impregnated with an acid to provide a pH of 1, 2, 3, 4, 5 or 6, and any of its intervals, to (i) favor the solubilization of at least a part of the starch, dextrin, disaccharides and / or monosaccharides contained in the bio-residue, (ii) to provide suitable conditions for cellulose, hemicellulose and lignocellulose and / or to sterilize the suspension. As used in the present invention, dextrin refers to low molecular weight mixtures of glucose polymers produced by hydrolysis of starch and linked by Į-1.4 and Į-1.6 bonds. The concentration of acid in the stream of clean bio-waste impregnated with acid can be adjusted to between 0.01 and 0.15 kg of acid per kg of


Clean bio-residue on a solid base. Mineral acids (for example sulfuric acid and hydrochloric acid) or organic acids may be used, and mineral acids are generally preferred.
5 Alternatively, the clean bio-waste can be optionally impregnated with a base. In particular embodiments, the base is ammonia and the concentration of ammonia is adjusted to 0.1, 0.5, 1, 1.5, 2 or 2.5 kg of ammonia per kg of clean bio-residue on a solid base, or adjusted between 0.1 and 2.5 kg of base per kg of clean bio-waste. In the impregnation with base, the concentration of water of the clean bio-waste
10 is adjusted to between 0.3 and 2.5 kg of water per kg of clean bio-residue in solid base, preferably between 1 and 2 kg of water per kg of clean bio-residue in solid base.
The impregnation of the clean bio-waste can be carried out by any means known in the art to achieve a substantially homogeneous mixture, including stirred mixing tanks (followed by a dehydration step), in-line mixers, kneading mixers, mixing mixers. paddles, tape mixers. In one method, the clean bio-wastes are sprayed with water (optionally comprising acid or base) with mixing in an elevated shear mixer, such as a belt mixer or a kneading type mixer. The impregnated material is normally maintained for a sufficient period of time before pretreatment at elevated pressure and temperature (for example, such as by steam contact) to allow equilibrium of humidity and temperature, such as about 1 to 20 minutes. In another method, a suspension comprising clean bio-waste, water (optionally comprising acid or base) 25 is formed by mixing at a moisture content of at least 60% by weight, preferably between 70% by weight and 90% by weight. weight. The suspension is then dehydrated to result in impregnated clean bio-waste. The final moisture content of the impregnated clean bio-waste is between 20% by weight and 80% by weight, preferably between 30% by weight and 70% by weight.
30 weight, and more preferably between 40% by weight and 60% by weight.
In the bio-waste suspension or the bio-waste suspension with adjusted pH, it is processed by at least one solid-liquid separation step to form a liquid stream comprising soluble components of bio-waste (for example, monosaccharides, disaccharides, dextrins and soluble starch) and a biomass stream


solid comprising insoluble bio-waste (for example, cellulose, hemicellulose, lignocellulose, minor amounts of dextrin and insoluble starch). Any solid-liquid separation technique known in the art, such as filtration or centrifugation, is suitable for said solid-liquid separation.
In a particular embodiment of the invention, at least a part of the liquid stream can be recirculated to the impregnation step. Additionally, at least a portion of the liquid stream may comprise at least a portion of an aqueous wash stream for washing the fine material with pretreated organic fiber and streams
10 of clean bio-waste (as described in the present invention). In another particular embodiment, at least a part of the liquid stream may comprise at least a portion of the aqueous wash stream for washing the stream of fine material enriched in bio-waste generated from the fractional stream enriched in rolling stock. Additionally, the pH of at least a part of the
The liquid stream can be adjusted at a suitable range for enzymatic hydrolysis and sent to the enzymatic hydrolysis stage (as described in the present invention).
In the hydrolysis of clean bio-waste described in the present invention, the current
20 solid biomass (optionally impregnated with water, acid or base) is optionally contacted with steam at a high temperature and pressure followed by rapid depressurization in a steam pretreatment stage to enhance the accessibility of the cellulosic components of the enzymes . More particularly, the solid biomass stream can be subjected to pressure conditions and
25 high temperatures to break up the cellulose-hemicellulose and cellulosehemicellulose-lignin complexes. After a period of contact time, the pressure of the solid biomass stream is reduced and / or the treated food is discharged to a reduced pressure environment, such as atmospheric pressure, to generate a stream of solid biomass treated with treated steam , and evaporate quickly and vent the steam.
30 The change in pressure results in a rapid expansion of the material that therefore helps to crumble the structure of the biomass fiber that includes, for example, the links between lignin (if present) and hemicellulose and / or cellulose in the cellulose-hemicellulose or cellulose-hemicellulose-lignin complex (collectively referred to as "cellulose complexes"). More particularly, by means
35 physicochemicals, steam treatment normally dissociates cellulose from the


hemicellulose and lignin (if present) providing cellulose suitable for enzymatic hydrolysis of glucose. Steam treatment normally dissociates hemicellulose from the complex, generally in the form of hemicellulose solubilized in a liquid phase of the treated cellulosic biomass. A part of the
The hemicellulose contained in the cellulosic biomass, such as from about 10% by weight to about 20% by weight, is solubilized in a liquid phase of the treated cellulosic biomass. Thus, the steam treatment provides hemicellulose suitable for the enzymatic hydrolysis of the monosaccharides.
The solid biomass or the impregnated biomass can be contacted with steam at a temperature between 100 ° C and 250 ° C, preferably from 150 ° C to 250 ° C and more preferably from 175 ° C to 220 ° C, even more preferably from 175 ° C to 200 ° C at a pressure between 100 kPa and 4,000 kPa, preferably from 300 kPa to 2,500 kPa, more preferably from 400 kPa to 1750 kPa, and even more preferably from 1000 kPa
15 to 1400 kPa. The total contact time is between 1 to 20 nm. In the case of high temperature and pressure, the total contact time is 1 to 5 minutes, and even more preferably 1 to 2 minutes. In some embodiments of the invention, the pressure is approximately 600 kPa and the contact time is approximately 8 minutes. In the present invention, after the time of
At 20 contact, the pressure is reduced to less than about 35 kPa, for example at 30 kPa, 25 kPa, 20 kPa, 15 kPa, 10 kPa or 5 kPa, slightly above ambient pressure, or approximately at ambient pressure to form the insoluble biomass pretreated with steam (i) in a single stage of pressure reduction or (ii) from about 345 kPa to about 1380 kPa, from about 345
25 kPa at approximately 1205 kPa, from approximately 690 kPa to approximately 1380 kPa, from approximately 690 kPa to approximately 1205 kPa, from approximately 690 kPa to approximately 1035 kPa, or from approximately 1035 kPa to approximately 1205 kPa in a first step of reducing pressure and hold it for a period of approximately 0.5 minutes at
About 20 minutes, followed by a reduction to less than about 35 kPa in a second stage.
The solid biomass stream (optionally impregnated with an acid or base) can be introduced into a container comprising a contact zone for steam treatment. The solid biomass stream is normally in the form


of a suspension, or cake. For example, the solid biomass stream can be pressed to form a cake, or an agglomerate of treated solids for introduction into the steam treatment vessel. The precise shape and configuration of the container are not very critical and can be selected by a person skilled in the art depending on the specific circumstances (for example, the properties of the cellulosic biomass and the operating conditions). In general, the vessel includes an inlet for the introduction of the solid biomass stream and one or more outlets to release the treated cellulosic biomass and / or the various components generated during steam treatment. Once the solid biomass stream is contained in the vessel, the vessel is pressurized and the solid biomass stream is heated by direct steam injection. In any of the various embodiments of steam pretreatment of the invention, a stream of steam or gas can be vented continuously or periodically from the steam pretreatment vessel to purge volatile organic compounds ("VOCs") 15 generated as by-products of the treatment with Cellulose, hemicellulose and lignocellulose vapor known to be fermentation and / or enzymatic inhibitor compounds. Such inhibitors include, for example, acetic acid, furfural and hydroxymethylfurfural ("HMF"). In particular embodiments of the invention, heating of the solid biomass stream can be carried out indirectly, such as by applying steam to a jacketed vessel. Typically, the solid biomass stream is maintained at a target temperature and pressure, such as by controlling the pressure, for a time sufficient to provide adequate heating. In particular embodiments of the present invention, after a period of pressurization of the container and heating of the solid biomass stream, the solid biomass stream is released or transferred from the contact vessel to a receiving vessel having a reduced pressure and controlled. In other particular embodiments of the present invention, after a period of pressurization of the container and the heating of the solid bio-waste stream, the pressure and temperature of the container is reduced to an intermediate pressure and temperature and maintained for a period of time. under those conditions, followed by a reduction in pressure or by a pressure slightly higher than atmospheric pressure. In other particular embodiments of the present invention, after a period of pressurization of the vessel and heating of the solid biomass stream, the pressure and temperature in the vessel is reduced at atmospheric pressure or at a pressure slightly higher than atmospheric pressure. In


Any of the embodiments of the present invention, as noted, the sudden decrease in pressure during this release promotes cellulose complex rupture. That is, the sudden decrease in pressure produces a rapid increase in the volume of steam and gases trapped inside the porous structure of the biomass which results in very fast incident gas velocities and / or rapid vaporization. of the heated water that has either occupied or been forced into the fibrous structure. In cases where the differential pressure is sufficiently high and where the pressure change occurs rapidly, the associated rapid vaporization and gas velocity occur
Essentially instantaneously in a method known in the art as steam explosion. In any of the embodiments of the present invention, the depressurization step generates a sudden vapor stream comprising various VOCs as described above.
In a particular embodiment in the ammonia treatment of the present invention, the clean bio-residue is first impregnated with water before adding it to a pretreatment reactor. The temperature can optionally be adjusted between 30 ° C and 80 ° C, preferably between 40 ° C and 60 ° C. The clean bio-waste impregnated with water is then added to a pretreatment reactor. The clean bio-waste
20 impregnated with water can be subjected to a partial vacuum in the pretreatment reactor to remove at least part of the trapped air. Anhydrous ammonia is preheated to provide the desired pressure and temperature. The pressurized and heated ammonia is then added to the pretreatment reactor and contacted with the clean bio-residue impregnated with water. The heat of
Dissolution of ammonia results in an increase in temperature. A person skilled in the art can determine the selection of the combination of (1) temperature and pressure of the anhydrous ammonia and (2) the water content of the clean bio-waste pretreated with water and the temperature necessary to achieve a predetermined pressure and temperature range. The contact time is properly between 5 and
30-20 minutes The temperature is suitably between 100 ° C and 250 ° C, preferably between 120 ° C and 200 ° C, more preferably between 140 ° C and 180 ° C. The pressure is suitably from 300 kPa gauge to 2500 kPa gauge, preferably 500 kPa gauge to 2000 kPa gauge, 700 kPa gauge to 1700 kPa gauge, or 850 kPa gauge to 1400
35 kPa gauge. Pressure is released quickly after a contact time


suitable for expanding cellulosic fibers.
In the present invention, the pretreated clean bio-residue is conditioned to form a suspension before coming into contact with a source of enzymes. In some embodiments, the pretreated clean biomass is contacted with a cooled aqueous stream, optionally comprising acid or base, to provide a suspension comprising stabilized monosaccharides, solubilized polysaccharides, insoluble compounds comprising cellulose, hemicellulose and / or lignocellulose, and non-fermentable material. When acid pretreatment is carried out, the cooled aqueous stream 10 may suitably be water with ammonia and, when base pretreatment is performed, such as the expansion of the fibers with ammonia, the cooled aqueous stream may suitably be a stream of mineral acid or an aqueous stream. In any of the embodiments, the cooled aqueous stream has a temperature of less than 20 ° C, less than 15 ° C or less than 10 ° C; The solids concentration of the pretreated clean bio-waste suspension is between 15% and 35% by weight, preferably between 20% by weight and 30% by weight, more preferably between 25% by weight and 35% in weigh; the pH of the pretreated clean bio-waste suspension is between 4 and 6, preferably between 4.5 to 5.5; and the temperature of the pretreated clean bio-waste suspension is
20 between 30 ° C and 70 ° C, preferably between 40 ° C and 60 ° C.
The pretreated clean conditioned bio-residue comprising a non-fermentable component is combined with an enzyme source comprising at least cellulase to generate a hydrolyzate comprising glucose and to remove at least one
25 stream of non-fermentable material.
The non-fermentable material comprises a mixture of components including one or more of wire, plastic, rope, glass, dirt, concrete, brick, metal objects (eg staples, nuts, bolts, nails, etc.), and paper loads. The non-fermentable components may comprise a mixture of milled and non-milled material. For example, at least a part of the non-fermentable material that comes from the through current of the first fractionation stage can be ground to produce pieces of wire, plastic, rope, glass, as well as other non-fermentable materials. At least a part of the non-fermentable material that comes from the sinking current of the first fractionation stage can be


Do not grind and include pieces of wire, plastic and rope, fabrics (for example, rags), as well as pieces of wire, plastic, rope, glass, and other non-fermentable material.
At least a part of the non-fermentable material normally comprises loads and
5 other compounds used in paper processing that separate from paper and cardboard during pretreatment and enzymatic hydrolysis. Paper fillers include clay (for example, kaolin clay - insoluble in water), calcium carbonate (slightly soluble in water and soluble in dilute acid) and other calcium-containing components, ink particles, titanium dioxide (insoluble in water and acid
10 diluted), talc (insoluble in water and slightly soluble in dilute mineral acid), components containing magnesium, components containing sodium, components containing potassium, components containing phosphorus and components containing aluminum. These components can be collectively referred to as "ash." Such ash particles normally have a
15 average particle size in the range of about 1 micrometer to about 5 micrometers. The paper also contains "adhesives" generally composed of polymeric aggregates and may include a mixture of, for example, glues, hot melt plastics, latex coatings and adhesives. Adhesives normally have an average particle size in the range of
20 about 1 micrometer to about 100 micrometers. The cellulosic component of paper and cardboard is generally characterized by an average fiber length of approximately 0.8 mm to approximately 1.2 mm for hardwood fibers, approximately 3 mm to approximately 7 mm for softwood fibers , and from about 1 mm to about 3 mm for fibers
25 non-wood vegetables.
The non-fermentable material component is characterized by a density range. Some part of the components are less dense than the enzyme hydrolysis suspension and separate (float) to the surface. Examples of some of said components include plastics and adhesives. Some part of the components has approximately the same density as the enzyme suspension and tend to remain suspended in a stirred suspension of enzymatic hydrolysis. Examples of some of said components include dust and ash (for example, ash components having particle sizes of about 100 microns or less). Some part of the components has a higher density than


Enzyme suspension and tend to separate (sink) towards the bottom of the enzyme hydrolysis suspension. Examples of some of said components include metals and rocks.
5 Cellulases are a class of enzymes produced mainly by fungi, bacteria, and protozoa that catalyze cellulolysis (hydrolysis) of cellulose into glucose, cellobiose, celotriose, celotetrose, celopentose, cellohexose, and longer chain celodextrins. Combinations of the three basic types of cellulases can be used. For example, endocellulases can be added to randomly hydrolyze the
10 ȕ-1,4, -D-glycosidic bonds in order to disrupt the crystalline structure of cellulose and expose the individual cellulose chains. Exocellulases can be added to cleave two units (cellobiose), three units (celotriose), or four units (celotetrose) from the exposed chains, while ȕ-glucosidase can be added to hydrolyze these compounds to glucose, which is available for fermentation .
Examples of suitable cellulases include, for example, Cellic® CTec2, Cellic® CTec3, CELLUCLAST®, CELLUZYME®, CEREFLO® and ULTRAFLO® (available from Novozymes A / S), LAMINEX®, SPEZYME®CP (Genencor Int.) , and ROHAMENT® 7069 W (Rohm GmbH), and GC-220 (Genencor International). The liquid stream is preferably sterilized to destroy microbes before being combined with the vapor of the
20 stream of pretreated solid biomass. Sterilization can be carried out by, for example, temperature treatment, UV radiation, or one of its combinations.
Usually, a suspension is formed from the pretreated clean bio-waste
25 conditioned in favorable conditions for cellulase activity. More particularly, the pH and temperature of the suspension is adjusted as described above and the content of suspended solids is adjusted between 15% by weight and 35% by weight, preferably from 25% by weight to 30 % by weight, with one or more between process water or aqueous wash streams described in the
30 present invention. The cellulase load in the suspension can be suitably varied with the cellulose content, but the typical load can be expressed as between 5 mg to 50 mg of cellulase per gram of cellulose, more preferably between 10 mg to 30 mg. of cellulase per gram of cellulose. In other words, the cellulase load is about 5 to about 50 mg of protein.
35 enzymatic per gram of cellulose in the treated cellulosic biomass.


The cellulase can be combined with the treated biomass suspension by any means known in the art to achieve a substantially homogeneous mixture, including stirred mix tanks, in-line mixers, 5 kneading mixers, paddle mixers, belt mixers, or in Liquefaction reactors such as reactors that have at least one mixing section and at least one piston flow section. The enzymatic hydrolysis reactor is normally a stirred vessel designed to maintain the biomass suspension cellulase mixture at a temperature suitable for cellulose hydrolysis 10 by cellulase, where the volume is sufficient to provide the maintenance time necessary for performance. Significant of hexose monosaccharide sugars derived from cellulose ("C6"), for example, glucose. In the present invention, the enzymatic hydrolysis vessel can be isolated and / or heated with a heating jacket to maintain the hydrolysis temperature. The cycle time of total enzymatic hydrolysis is 48 hours to 144 hours, and its intervals are within the scope of the present invention. Glucose yields, based on the total cellulose content of the biomass suspension are normally from about 30% to about 90%, from about 40% to about 80% from about 30% to 20 about 70% or from about 60 % to approximately 75% of
theoric value.
For very viscous treated biomass suspensions, such as those with
a viscosity greater than 20,000 cP, or about 500,000 cP, mixing with enzymes can be carried out in two stages. In a first stage, cellulase
it can be mixed with the biomass in a mixer particularly suitable for
processing of very viscous materials, for example, a kneading type mixer,
a paddle mixer (single or double shaft), or a belt mixer (shaft
single or double). High viscosity mixers are particularly suitable for the method of the present invention because the vigorous stirring
of the cellulase with the viscous suspension of the treated biomass allows a rapid
viscosity reduction in the subsequent stage of liquefaction where the viscosity is
preferably reduces to less than about 20,000 cP, less than
about 15,000 cP, less than about 10,000 cP or even less than 35 of about 5000 cP. The high viscosity mixer can have


optionally a jacket to receive a cooling or heating means in order to maintain the temperature of the treated biomass during the addition of cellulase. Optionally, a cooling and heating means can be incorporated in the components of the internal mixer (such as in the rotation shafts, vanes) to further enhance the heat exchange. The cellulase can be added by means of one or more addition points, for example, multiple spray nozzles, near the inlet of the treated biomass. In a second stage, the treated biomass-cellulase mixture can be processed in a mixing tank or in a fiber liquefaction bioreactor. The treated biomass-cellulase mixture 10 can be processed in a fiber liquefaction bioreactor to further reduce the viscosity before transfer to a cellulose hydrolysis reactor. The fiber liquefaction bioreactor can be either a continuous mixing design or a design with at least one continuous mixing section and at least one piston flow section. Optionally, two or more fiber liquefaction bioreactors can be operated in series. The fiber liquefaction bioreactor comprises alternating mixing zones and proximal piston flow zones and the treated biomass-cellulase mixture flows both down through the tower by gravity or is moved up by pumping. The treated biomass-cellulase mixture is normally processed in a fiber liquefaction bioreactor until
The viscosity of the mixture is less than about 10,000 cP, less than about 9,000 cP, less than about 8,000 cP, less than about 7,000 cP or less than about 5000 cP where it is then transferred to a cellulose hydrolysis reactor.
25 Enzymatic hydrolysis proceeds with the parallel removal of non-fermentable material from the suspension. Suitable disposal methods include, without limitation, flotation, foam removal, filtration, screening, hydraulic classification, rakes, removal of dense solids from a collapsible ramp of residual material leaving the bottom of the enzymatic hydrolysis vessel, tweezers, trap doors
30 waste, extraction tubes.
At least a part of the non-fermentable material can be separated in the direction of the surface of the enzyme hydrolyzate suspension. At least a portion of the floating non-fermentable material can be removed by foam removal. Suitable foam removers with known in the art and include


floating foam foam eliminators in which the floating material passes over a dam and is collected. Other foam removers include oleophilic foam removers, where the floating material adheres to a rotating element, such as a drum or tape, in contact with the surface layer, and the material that adheres to the element is removed and collected. Other additional foam removers include tube foam removers and membrane foam removers. Any of the different foam removers can be used together with a waste rake or other means to remove floating objects such as pieces of plastic and cloth. The collected liquid fraction can be
10 optionally process in an oil concentrator or oil-water separator to remove residual aqueous material for recycling to the enzymatic hydrolysis vessel and / or process purge.
Foam removal can be used together with flotation to improve the
15 foam removal efficiency. Flotation can be carried out suitably by bubbling pressurized air through the suspension to cause suspended non-fermentable particulate to float to the surface. Flotation can also be carried out by dissolved gas flotation, where dissolved gas is released at atmospheric pressure in the hydrolysis tank and can also be
20 use to fractionate the suspended non-fermentable material and the gas can be air. In some embodiments of the present invention, the gas is carbon dioxide generated during fermentation. It is believed that the use of residual fermentation gases comprising carbon monoxide for flotation improves glucose performance compared to air flotation by reducing oxidation
25 of the monosaccharides.
At least a part of the non-fermentable material may be suspended in the enzyme hydrolyzate suspension. At least a part of the suspended non-fermentable material can be removed by recirculation through a sieve or filter having 30 openings of 2 mm to 30 mm, preferably 8 mm to 20 mm or 4 mm to 10 mm in which at At least a part of the non-fermentable material is retained in the sieve or filter and separated from the enzyme suspension, in which the rest of the enzyme suspension crosses the sieve. The enzyme suspension filtrate that passes through the sieve can contain fine non-fermentable material such as ash, adhesive components and powder. The coarse fraction may contain soluble sugars that may


be recovered in a solid washing stage. The fine fraction is filtered a second time by a screen with mesh light between 1 mm and 0.1 mm, where the soluble fraction passes through the screen and non-fermentable solids, such as plastics and fibers, are retained in the sieve and / or pass through the sieve and are recycled to the enzyme hydrolysis vessel 5. The fraction retained in the second screen comprises insoluble non-fermentable fine solids and may contain soluble monosaccharides, polysaccharides and enzyme. The retained fraction can be washed with any of the aqueous streams obtained at some point in the process of the present invention to recover soluble sugars and enzyme. Some examples of suitable apparatus 10 for liquid solid separation are the press filter, rotary filter, band filters, centrifuges or the combination thereof. At least a part of the dense non-fermentable material can be separated in the direction of the bottom of the enzyme hydrolyzate suspension. The enzymatic hydrolysis vessel has a conical bottom with an outlet in the lower elevation and at least one outlet in an upper elevation, such as in the transition between the side wall and the conical bottom. At least a part of the dense material can be removed from the enzyme hydrolysis vessel by passing the suspension through the outlet located in the lower elevation through a sieve having openings of 4 mm to 30 mm, preferably 4 mm to 20 mm or from 4 mm to about 10 mm to collect the dense non-fermentable material on the sieve 20 as a through stream and recycle the filtrate into the enzyme hydrolysis vessel. In some embodiments of the present invention, a conveyor with dehydration is used to remove dense (heavy) non-fermentable material from the outlet located at the bottom of the enzymatic hydrolysis vessel with a recycled aqueous stream for recovery. Additionally, the enzyme hydrolysis vessel
25 comprises a bucket with tweezers in the lower section into which the dense non-fermentable material is separated and a bucket elevator to remove the bucket for cleaning.
Other auxiliary equipment can be used to remove non-fermentable material from the
30 enzymatic hydrolysis vessel. For example, rakes can be used to remove debris such as wires, plastic, rope, cloth, etc.
Optionally, additional enzymes such as a hemicellulase (for example, a xylanase to further hydrolyze the various types of hemicellulose to 35 xylose), a Į-amylase (to liquefy the free starch that is trapped


previously in the cellulose, hemicellulose and / or lignocellulosic matrices, a ȕamylase, a glucoamylase (to convert the liquefied starch into C6 sugars), an arabinoxylanase, a pululanase, and / or a protease (to hydrolyze the peptide bonds and release granules of starch embedded in the protein matrix) can be added to the treated cellulosic biomass to generate additional C6 sugars and / or pentose sugars ("C5"). Non-limiting examples of C6 sugars include glucose, galactose, mannose, and fructose and non-limiting examples of C5 sugars include xylose, arabinose and ribose. The optional enzymes can be mixed with the cellulosic biomass treated at any time during hydrolysis including with the
10 cellulase during a high viscosity mixture, in one or more locations of the fiber liquefaction bioreactor and / or in the cellulose hydrolysis reactor.
A hemicellulase, as used in the present invention, refers to a polypeptide that can catalyze the hydrolysis of hemicellulose in small polysaccharides such as oligosaccharides, or monosaccharides including xylose and arabinose. Hemicellulases include, for example, the following: endoxylanases, ȕ-xylosidases, Į-L-arabinofuranosidases, Į-D-glucuronidases, feruloyl esterases, coumarolyl esterases, Į galactosidases, ȕ-galactosidases, ȕ-mannases, and ȕ-mannosidases. A xylanase can be obtained from any suitable source, including fungal and bacterial organisms, such as Aspergillus, Disporotrichum, Penicillium, Neurospora, Fusarium, Trichoderma, Humicola, Thermomyces, Myceliophtora, Crysosporium, and Bacillus. Commercially available preparations comprising xylanase include SHEARZYME®, BIOFEED WHEAT®, BIO-FEED Plus®L, ULTRAFLO®, VISCOZYME®, PENTOPAN MONO®BG, and PULPZYME®HC
25 (Novozymes A / S), and LAMINEX® and SPEZYME®CP (Genencor Int.) An example of a hemicellulase suitable for use in the present invention includes VISCOZYME® (available from Novozymes A / S, Denmark).
Generally, any of the protease classes are applicable, for example,
30 acidic, basic or neutral, and proteases of, for example, Novozymes, Genencor and Solvay are commercially available. Examples include, for example, GC106 (available from Genencor International), AFP 2000 (available from Solvay Enzymes, Inc.), FermGen ™ (which is an alkaline protease available from Genencor International), and Alcalase® (which is an acid protease available from Novozymes Corporation). A
35 commercially available pululanase is Promozyme® D2, available from Novozymes


Corporation Commercially available compositions comprising glucoamylase include: AMG 200L, AMG 300 L, AMG E, SAN® SUPER, SAN® EXTRA L, SPIRIZYME® PLUS, SPIRIZYME® FUEL, SPIRIZYME® FG and SPIRIZYME® E (all available from Novozymes); OPTIDEX® 300 and DISTILLASE® L-400 5 (available from Genencor Int.); and G-ZYME ™ G900, G-ZYME ™ 480 Ethanol and G990 ZR (available from Genencor Int.). Examples of commercial acid Į-amylases of the invention include TERMAMYL® SC, LIQUOZYME® SC DS, LIQUOZYME® SC 4X, and SAN ™ SUPER (all available from Novozymes AJS, Denmark); and DEX-LO®, SPEZYME® FRED, SPEZYME® AA, and SPEZYME® DELTAAA (all available from
10 Genencor).
Also useful are multienzyme complexes containing multiple carbohydrases, such as Viscozyme® L, available from Novozymes Corporation, containing arabanase, cellulase, ȕ-glycanase, hemicellulase, and xylanase.
In the present invention, monosaccharides can be extracted or otherwise separated from hydrolyzed biomass. Hydrolyzed biomass can be introduced into a sugar recovery apparatus comprising a suitable solid / liquid separation equipment such as, for example, a sieve, filter, centrifuge,
20 settler, percolator, extraction column, flotation vessel, or one of its combinations, to generate a liquid fraction comprising monosaccharide sugars and a solid fraction, where the solid fraction may suitably be in the form of a cake or suspension . The solids fraction can be washed one or more times for the recovery of additional monosaccharides. The
Monosaccharides can be recovered from the solid fraction by countercurrent contact of the solid fraction with a washing liquid in a suitable apparatus to form a washing stream comprising the extracted monosaccharides. The liquid fraction is combined with a liquid medium and / or the wash streams to form a monosaccharide fraction. The precise composition of the liquid medium and the
30 wash liquids are not strictly critical. However, in preferred embodiments of the present invention, the liquid medium and the washing liquid can process water if a relatively high purity monosaccharide fraction is desired. Although the precise composition of the monosaccharide fraction varies with the biomass composition, generally, the monosaccharide compositions
35 comprise at least about 5% by weight, at least about


6% by weight, at least about 7% by weight, at least about 8% by weight, at least about 9% by weight, or at least about 10% by weight of monosaccharides. The residual solids fraction comprises a non-hydrolyzed cellulose, non-hydrolyzed hemicellulose, non-hydrolyzed lignocellulose,
5 polysaccharides (for example, starch granules), entrained monosaccharides and lignin. The residual solids fraction can be recycled properly for the recovery of sugars and sugary substrates.
The monosaccharide stream can be concentrated to produce a concentrate or
10 syrup with a content of at least 25% by weight, at least 40% by weight or at least 60% by weight. The methods for concentrating this current may be those known in the state of the art and include evaporators, reverse osmosis or the combination thereof, among others. This monosaccharide stream can be concentrated in two steps to solids concentrations from 50%
15 to about 70% by weight or about 40% to about 80% by weight.
In a first step in the concentration of monosaccharides can be carried out at a temperature from about 50 ° C to 100 ° C or from 70 ° C to 80 ° C
20 approximately. In another embodiment of the present invention either of the two evaporation stages or both are carried out under vacuum conditions.
Any of various streams of solid biomass treated with enzymes, suspension streams containing fermentable sugars and aqueous streams containing fermentable sugars can be used with suitable microorganisms as a substrate for the production of fermentation products. A wide variety of fermentation microorganisms are known in the art, and others can be discovered, produced by mutation, or engineered by recombinant means. The fermentation microorganisms within the scope of the present invention include yeasts, bacteria, filamentous fungi, microalgae, and combinations thereof. Examples of fermentation products within the scope of the present invention include, for example, acids, alcohols, alkanes, alkenes, aromatics, aldehydes, ketones, triglycerides, fatty acids, biopolymers, proteins, peptides, amino acids, vitamins, antibiotics, Pharmacists, and their combinations.


Non-limiting examples of alcohols include methanol, ethanol, propanol, isopropanol, butanol, ethylene glycol, propanediol, butanediol, glycerol, erythritol, xylitol, sorbitol, and combinations thereof. Non-limiting examples of acids include acetic acid, lactic acid, propionic acid, 3-hydroxypropionic acid, butyric acid, acid.
5 gluconic acid, itaconic acid, citric acid, succinic acid, levulinic acid, and combinations thereof. Non-limiting examples of amino acids include glutamic acid, aspartic acid, methionine, lysine, glycine, arginine, threonine, phenylalanine, tyrosine, and combinations thereof. Other examples of fermentation products include methane, ethylene, acetone and industrial enzymes.
10 Fermentation organisms may be natural microorganisms or recombinant microorganisms, and include Escherichia, Zymomonas, Saccharomyces, Candida, Pichia, Streptomyces, Bacillus, Lactobacillus, and Clostridium. The fermentation organism can be recombinant Escherichia coli, Zymomonas
15 mobilis, Bacillus stearothermophilus, Saccharomyces cerevisiae, Clostridia thermocellum, Thermoanaerobacterium saccharolyticum, or Pichia stipites. In some embodiments of the present invention, the microorganism is a microalgae, defined as a eukaryotic microbial organism that contains a chloroplast or plastid, and which is optionally capable of carrying out photosynthesis, or a microbial organism.
20 prokaryotic capable of carrying out photosynthesis. Microalgae include obligate photoautotrophs, which cannot metabolize a fixed carbon source as energy, as well as heterotrophs, which can only live from a fixed carbon source. Microalgae include single-celled organisms that separate from sister cells that shorten after cell division, such as Chlamydomonas, as well.
25 as microbes such as, for example, Volvox, which is a simple multicellular photosynthetic microbe of two different cell types. Microalgae include cells such as Chlorella, Dunaliella, and Prototheca. Microalgae also include other microbial photosynthetic organisms that exhibit cell-cell adhesion, such as Agmenellum, Anabaena, and Pyrobotrys. The microalgae also include
30 forced heterotrophic microorganisms that have lost the ability to carry out photosynthesis, such as in certain species of algae of dinoflagellates and species of the Prototheca genus.
Non-limiting examples of fermentative organisms and their associated product 35 include the following. The fermentation of carbohydrates is known to give


acetone, butanol and ethanol by: (i) solvent isogenic Clostridia as described by Jones and Woods (1986) Microbiol. Rev. 50: 484-524; (ii) a mutant strain of Clostridium acetobutylicum as described in US Patent No. 5,192,673; and (iii) a mutant strain of Clostridium beijerinckii is known as described in US Patent No. 6,358,717. The fermentation of carbohydrates to ethanol by modified strains of E. coli has been described by Underwood et al., (2002) Appl. Environ. Microbiol. 68: 6263-6272 and by a genetically modified strain of Zymomonas mobilis described in US 2003/0162271 A1. The preparation of lactic acid is known from recombinant strains of E. coli (Zhou et al., (2003) Appl. Environ. Microbiol. 69: 399-407), natural strains of Bacillus (US20050250192), and Rhizopus oryzae (Tay and Yang (2002) Biotechnol. Bioeng. 80: 1-12). Recombinant E. coli strains have been used as biocatalysts in fermentation to produce 1.3 propanediol (US Pat. No. 6,013,494 and 6,514,733) and adipic acid (Niu et al., (2002) Biotechnol. Prog. 15 18: 201-211). Acetic acid has been produced using recombinant Clostridia (Cheryan et al., (1997) Adv. Appl. Microbiol. 43: 1-33) and recombinant strains have recently been identified (Freer (2002) World J. Microbiol. Biotechnol. 18: 271-275). The production of succinic acid by recombinant E. coli and other bacteria is described in US Patent No. 6,159,738 and by recombinant E. coli in Lin et al., (2005) Metab. Eng. 7: 116-127). Pyruvic acid has been produced by mutant Torulopsis glabrata yeast (Li et al., (2001) Appl. Microbiol. Technol. 55: 680-685) and by mutant E. coli (Yokota et al., (1994) Biosci. Biotech Biochem. 58: 2164-2167). Recombinant E. coli strains have been used for the production of parahydroxycinnamic acid (US20030170834) and acid
25 quínico (document US20060003429).
Amino acid production has been carried out by fermentation using auxotrophic strains and resistant strains similar to Corynebacterium, Brevibacterium, and Serratia amino acids. For example, the production of histidine 30 using a strain resistant to a histidine analog is described in Japanese Patent Publication No. 8596/81 and it is described to use a recombinant strain in EP 136359. Production of tryptophan using a strain resistant to a tryptophan analog in Japanese patent publications Nos 4505/72 and 1937/76. The production of isoleucine is described using a strain resistant to an isoleucine analog in Japanese patent publications Nos. 38995/72,


6237/76, 32070/79. The production of phenylalanine is described using a strain resistant to a phenylalanine analog in Japanese Patent Publication No. 10035/81. Tyrosine production has been described using a strain that requires tyrosine-resistant phenylalanine for growth (Agr. Chem. Soc. Japan 50 (1) 5 R79-R87 (1976)), or a recombinant strain (EP263515, EP332234) , and the production of arginine using a strain resistant to an analogue of L-arginine (Agr. Biol. Chem. (1972) 36: 1675-1684, Japanese patent publications Nos 37235/79 and 150381/82). produced phenylalanine by Eschericia coli strains ATCC 31882, 31883, and 31884. The production of glutamic acid in a recombinant Coryneform bacterium is described in US Patent No. 6,962,805. The production of threonine by a mutant strain is described. E. coli in Okamoto and Ikeda (2000) J. Biosci Bioeng. 89: 87-79. Methionine has been produced by a mutant strain of Corynebacterium lilium (Kumar et al, (2005) Bioresour. Technol. 96: 287294). The production of peptides, enzymes, and other proteins is also known for 1 5 microorganisms as described in U.S. Patent Nos. 6,861,237,
6,777,207 and 6,228,630. The production of triglycerides, fatty acids and fatty acid esters (e.g., biodiesel) by microalgae is also known as described in U.S. Patent Nos. 7,883,882, 8,187,860, 8,278,090 and 8,222,010, and in published US patent applications numbers 20 20100303957, 20110047863 and 20110250658.
The selection of suitable fermentation conditions can be carried out suitably by those skilled in the art based on (i) the identity of the microorganisms or a combination of microorganisms, (ii) the characteristics of the substrate medium for fermentation and (iii) the associated fermentation product. Fermentation can be aerobic or anaerobic. Single and multistage fermentations are within the scope of the present invention. The fermentation substrate medium may be supplemented with additional nutrients necessary for microbial growth. Supplements may include, for example, yeast extract, vitamins, growth promoters, specific amino acids, phosphate sources, nitrogen sources, chelating agents, salts, and trace elements. The components necessary for the production of a specific product prepared by a specific microorganism, such as an antibiotic to maintain a plasmid or a cofactor necessary in a reaction catalyzed by enzymes, can also be included. Additional sugars may also be included to increase the


Total sugar concentration Adequate fermentation conditions are achieved by adjusting these types of factors for growth and for the production of the target fermentation product by a microorganism. The fermentation temperature can be any temperature suitable for the growth and production of the 5 nutrients of the present invention, such as between about 20 ° C to about 35 ° C. The pH of the fermentation can be adjusted or controlled by adding an acid or base to the fermentation mixture. In such cases, when ammonia is used to control the pH, it also conveniently serves as a source of nitrogen. The fermentation mixture may optionally be maintained to have a dissolved oxygen content during the course of fermentation to maintain cell growth and to maintain a cellular metabolism for nutrient production. The oxygen concentration of the fermentation medium can be controlled using known methods such as by the use of an oxygen electrode. Oxygen can be added to the fermentation medium using methods known in the art such as by agitation and aeration of the medium by agitation, shaking or use of bubblers. Fermentation can occur after enzymatic hydrolysis or it can occur concurrently with enzymatic hydrolysis by Saccharification and Simultaneous Fermentation (SSF). SSF can maintain sugar levels produced by
20 hydrolysis thus reducing the potential inhibition of the product of the enzymes of the hydrolysis, reducing the availability of sugar for contaminating microorganisms, and improving the conversion of the treated biomass to monosaccharides and / or oligosaccharides.
25 Hexose sugar fermenting organisms include yeasts. Any variety of yeasts can be used as yeast in the present method. Typical yeasts include any of a variety of commercially available yeasts, such as commercial strains of Saccharomyces cerevisiae. Suitable commercially available strains include ETHANOL
30 RED (available from Red Star / Lesaffre, USA); BioFenn HP and XR (available from North American Bioproducts); FALI (available from Fleischmann's Yeast); SUPERSTART (available from Lallemand); GERT STRy (available from Gert StrandAB, Sweden); FERMIOL (available from DSM Specialties); and Thennosac (available from Alltech). In some embodiments, the hexose fermenting organism is a yeast.
Recombinant that has at least one transgene that expresses an enzyme useful for


convert mono and / or oligosaccharides into ethanol.
In particular embodiments of the present invention directed to the generation of organic compounds, preferably ethanol, by yeasts, the fermentation medium has a pH of about 3.5 to about 6, about 3.5 to about 5 or about 4 to about 4.5. If a pH adjustment is required, mineral acids such as sulfuric acid, hydrochloric acid or nitric acid, or bases such as ammonia (ammonium hydroxide) or sodium hydroxide can be used. To enhance the efficiency of ethanol fermentation and an increase in ethanol yield, additional nutrients can be added to enhance yeast proliferation. Such nutrients include without limitation, free amino nitrogen (FAN), oxygen, phosphate, sulfate, magnesium, zinc, calcium, and vitamins such as inositol, pantothenic acid, and biotin. Typical sources of FAN include urea, ammonium sulfate, ammonia, amino acids, and Į-amino nitrogen groups of peptides and proteins. The added FAN content is preferably from about 1.2 to about 6 mg N / g of starch, for example 1.2, 2.4, 3.6, 4.8 or 6 mg N / g of starch. In the case of urea, it is preferred to add between about 2.4 to about 12 mg of urea per gram of starch, for example, 2.4, 4.8, 7.2, 9.6 or 12 mg of urea per gram of starch The 20 food yeasts that supply, for example, vitamins (such as vitamins B and biotin), minerals (such as magnesium and zinc salts and micronutrients and nutrients can be added to the fermentation medium) Food yeasts can include an autolysed yeast and extracts of plants and are usually added at a concentration of about 0.01 to about 1 g / l,
25 for example, from about 0.05 to about 0.5 g / l. Bactericides can also be optionally added to the fermentation medium. Examples of typical bactericides include virginiamycin nisin, erythromycin, oleandomycin, fiavomycin, and penicillin G. In the case of virginiamycin, a concentration of about 1 ppm to about 10 ppm is preferred.
30 Suitable pentose sugar fermentation organisms (eg, xylose) include yeasts. Such yeasts include Pachysolen tannophilus, Pichia stipites, Candida diddensii, Candida utilis, Candida tropicalis, Candida subtropicalis, Saccharomyces diastaticus, Saccharomycopsis fibuligera and Torula candida. In some
35 embodiments, the pentose fermenting organism is a recombinant yeast


which has at least one transgene that expresses an enzyme useful for converting mono and / or oligosaccharides into ethanol. For example, the genome of P. stipites can be incorporated into
S. cerevisiae using a gene redistribution method to produce a
hybrid yeast capable of producing bioethanol from xylose while retaining the ability to survive high concentrations of ethanol.
In some embodiments of the present invention, organisms capable of fermenting both hexose and pentose sugars are used to convert monosaccharides into ethanol. Typically, such organisms are strains of S. cerevisiae that have
10 transgenes encoding one or more enzymes capable of converting pentose sugars to ethanol.
In the present invention where the fermentation medium comprises cellulosic biomass treated with enzymes comprising non-hydrolyzed cellulosic material such as cellulose, hemicellulose, lignocellulose, and fragments thereof, the source of the fermentation organism may optionally comprise at least one species of cellulolytic organism capable of breaking down and metabolizing non-hydrolyzed cellulose, hemicellulose and / or lignocellulose present in the ethanol fermentation medium. Such cellulolytic organisms are known in the art and include Escherichia coli, 20 Zymomonas mobilis, Bacillus stearothermophilus, Saccharomyces cerevisiae, Clostridia thermocellum, Thermoanaerobacterium saccharolyticum, Pichia stipites and Pachysolen tannophilus. Also within the scope of the present invention are cellulolytic bacteria that have one or more transgenes encoding the ethanol producing route. In some embodiments of the present invention the source of the
The fermentation organism further comprises at least one species of cellulolytic organism capable of breaking the non-hydrolyzed hemicellulose present in the adjusted combined liquefaction mixture and capable of synthesizing ethanol.
Fermentation products can be recovered using any of
30 various methods known in the art. For example, fermentation products can be separated from other fermentation components by distillation (for example, azeotropic distillation liquid-liquid extraction, solid-liquid extraction, adsorption, gas entrainment, membrane evaporation, pervaporation, centrifugation, crystallization , filtration, microfiltration, nanofiltration, exchange
35 ionic, or electrodialysis). As a specific example, methanol, ethanol, or other products


Fermentation having sufficient volatility can be recovered from a fermentation mixture by distillation. In another example, 1-butanol can be isolated from a fermentation mixture using methods known in the art for acetone-butanol-ethanol ("ABE") fermentations (see, for example, Durre, Appl. 5 Microbiol. Biotechnol. 49 : 639-648 (1998), Groot et al., Method. Biochem. 27: 61-75 (1992), and references cited therein), for example, by removing solids followed by isolation by distillation, extraction liquid-liquid, adsorption, gas entrainment, membrane evaporation, or pervaporation. In yet another example, 1,3-propanediol can be isolated from a fermentation mixture 10 by extraction with an organic solvent, distillation, and column chromatography (see US Patent No. 5,356,812). In yet another example, amino acids can be collected from the fermentation mixture by methods such as adsorption by ion exchange resin and / or crystallization. One skilled in the art can carry out the selection of a separation method suitable for
15 any concrete fermentation product.
In accordance with the present invention, any of the rich organic fractions such as clean bio-waste, organic fiber, insoluble insulated biomass, streams rich in combustible material and / or CSR, can be converted into by-products 20 by gasification methods or fermentation methods of Gas synthesis gas known in the art. In the gasification methods, the rich organic fraction is heated at high temperature in an atmosphere with oxygen supply or in the essential absence of oxygen to produce synthesis gas (mainly hydrogen and carbon monoxide) which is subsequently reacted to form a stream. of gas comprising one or more carbon compounds. For example, in a step of synthesis of Fischer-Tropsch ("FT"), H2 and CO in synthesis gas are reacted on a catalyst (for example, iron or cobalt) to form a wide range of hydrocarbon chains of various lengths The FT reaction is usually carried out at a pressure of about 20 bar to about 40 30 bar in a temperature range of either about 200 ° C to about 250 ° C or from about 300 ° C to about 350 ° C. Iron catalysts in the upper temperature range are generally used to produce olefins for a lighter gasoline product and cobalt catalysts at a lower temperature range to produce longer chains that can be cracked to diesel. The production of methanol from synthesis gas


It usually involves reacting CO, H2 and a small amount of CO2 on a zinc-copper oxide catalyst where the reaction takes place via reaction by displacement with water followed by the hydrogenation of CO2. The process is usually carried out at a pressure of about 50 to about 100 bar (10 MPa) and in a temperature range of about 220 ° C to about 300 ° C. The synthesis of mixed alcohols from synthesis gas is similar to both FT and methanol synthesis that uses modified catalysts from those methods with the addition of alkali metals to promote the mixed alcoholic reaction, where the molar ratio of H2 to CO is from
10 about 1: 1 to about 1.2: 1.
In synthesis gas fermentation methods, a variety of microorganisms can use synthesis gas as a source of energy and carbon to produce fermentation products such as ethanol, butanol, acetate, formate and butyrate. Such organisms include Acetobacterium woodii, Butyribacterium methylotrophicum, Clostridium carboxidivorans P7, Eubacterium limosu, Moorella and Peptostreptococcus productus. For example, certain anaerobic microorganisms can produce ethanol and other useful CO products by fermentation. For example: US Pat. No. 5,173,429 describes Clostridium ljungdahlii ATCC No. 49587, 20 an anaerobic microorganism that produces ethanol from synthesis gas; U.S. Patent No. 5,807,722 describes a method and an apparatus for converting the synthesis gas into organic acids and alcohols using Clostridium ljungdahlii ATCC No. 55380; U.S. Patent No. 6,136,577 describes a method and apparatus for converting synthesis gas into ethanol using Clostridium 25 ljungdahlii ATCC No. 55988 and 55989; The US publication No. 20070275447 describes a bacterial species of clostridium (Clostridium carboxidivorans, ATCC BAA-624, "P7") that can synthesize biofuels from synthesis gas; and the US patent. No. 7,704,723 describes a bacterial species of Clostridium (Clostridium ragsdalei, ATCC BAA-622, "P11") that can synthesize 30 biofuels from waste gases. US publication 20140120591 describes a species of acidogenic Clostridium tyrobutyricum (ITRI04001) that can synthesize volatile fatty acids (eg, formic acid, acetic acid, lactic acid, propanoic acid, butyric acid, and mixtures thereof) from synthesis gas. The fermentation conditions are usually of atmospheric pressure at 2 bar (200 kPa), and at a temperature range of approximately


15 ° C to approximately 55 ° C, with the selection of specific fermenter conditions and pH depending on the fermenting microorganism.
This written specification uses examples to disclose the invention, including5 the best way, and they also allow anyone skilled in the field to takepractice the invention, including the preparation and use of any devices
or systems and carry out any built-in methods. The patentable scope of the invention is defined by the claims, and may include other examples that occur to the person skilled in the art. These other examples are intended for
10 be within the scope of the claims if they have elements that do not differ from the literal language of the claims, or if they include equivalent elements with minor differences from the literal language of the claims. 15 PREFERRED EMBODIMENT OF THE INVENTION
Non-limiting embodiments of the present invention are plotted in Figure 1.
20 Figure 1 graphically represents, by means of a block diagram, the Separation and Classification area of the MSW as well as the Cleaning of the bio-waste, a previous step necessary to obtain a bio-residue rich in cellulosic compounds that will become monosaccharides.
The mixture of Urban Solid Waste (1) is optionally processed by a manual sorting stage, by manual triage (101) in which large or bulky waste and / or hazardous materials (MV) are removed, a current is obtained (2 ) rich in recyclable materials (MR) (such as paper and cardboard) and other current (3) that goes to the next stages.
The stream (3) is fractionated into a first sorting stage (102) (for example, trommel) having a sieve opening of about 60 mm to about 100 mm to form a first through-stream (5) rich in material recyclable and a first sink current (4) rich in organic matter
35 fermentable.


The first pass-through current (5) may optionally be subjected to a new step of size classification by means of a trombone (103) to be divided into a second sinking current (6) of small size, and a second current
5 intern (7) large.
The stream (6), is subjected to a new separation stage in a ballistic separator (104), which separates the material according to its density, size and shape, being classified into a stream of fine material (8), a stream of rolling material
10 (9) and a stream of planar material (10). The stream of fine material (8) is enriched in organic matter, while the stream of rolling stock (9) is enriched in recyclable plastic material and that of planar (10) is enriched in paper and cardboard among other two-dimensional materials.
15 The fine stream (8) joins the first sink current (4) to form the raw bio-waste stream and passes through a magnetic separator (105) and a Foucault separator (106) and the generated current (11 ) can be fractionated with a trombone (107) to form a third sunken stream enriched with inert compounds (13) and a third through current (12) having at least 40% in
20 weight of organic matter and also comprising a recyclable component comprising plastic. The sinking current rich in inorganic material (13) can be purged from the process by a conditioning step (126) and the generated current (23) is divided by an optical separator (127) for the recovery of glass (v).
The third through current (12) is fractionated by density separation, in a densimetric (108) to form a first dense current (15) and a first light current (14). The light stream (14) is fractionated with a trombone (110) to form a fourth sinking stream (16) enriched in bio-waste and a fourth
30 through current (17) enriched in recyclable material. The fourth through current
(17) it is divided by optical system (113) into a stream rich in organic material (19) comprising plastics, metals, glass, paper and / or cardboard and into a recovered stream enriched in plastic (18) that passes into a system for the recovery of recyclable material (114).


The first dense stream (15) is enriched in inorganic compounds, glass and metal is passed through a Foucault separator (109) to separate the metals from a stream (22) that is taken along with the stream (13) to a stage of conditioning (126).
5 The fourth sinking current (16) enriched in bio-wastes is fractionated using an X-ray classification system (111) inert (r) are eliminated, mainly composed of stones, bones or sands and the clean current joins the rich current in organic matter (19) and pass to a mill (112) to
10 reducing its size to a particle average of less than approximately 25 mm, giving rise to the bio-waste stream (20), a clean bio-waste stream for conversion into monosaccharides.
The rolling stock stream (9) is subjected to a metal extraction passing
15 by a magnetic separator (115) obtaining a stream of recyclable material (21) free of metals that passes through a recovery system of recyclable material (114), together with the current (18) above. The rejection current (24) of said process can feed, together with the second through current (7), to the current (25) for obtaining CSR, by passing through a manual triage (116). The current
20 (25) is separated by air classification as described in the present invention (such as a linear air separator) (117) to form a second light stream (26) and a second dense stream (27) in which the Light current is enriched in combustible material compared to heavy current. The light stream (26) is processed in a crushing step (118) for additionally
25 reduce the volume and particle size and form the CSR (35).
The planar material stream (10) is passed through an optical separator (119) to obtain a stream (38) of paper and cardboard (P&C) and a remainder stream (34) enriched in planar combustible material. Optionally the current (10) can be
Grind before, after or in parallel of the mixture with the aqueous solution (36).
The bio-waste stream (20) is the clean bio-waste stream for conversion into monosaccharides. This clean bio-waste stream (20) comprises fermentable material and is enriched in fermentable material. The stream of 35 clean milled bio-wastes is impregnated with a stream of water, acid (solution


aqueous) or base (aqueous solution) (36) in an impregnation vessel (120) to form a stream of impregnated bio-waste (28) and is used directly as a pretreatment feed (121) where it is pretreated at elevated pressure and temperature, such as by contact with steam, followed by a rapid release of pressure to form the pretreated bio-waste (29). The pretreated bio-waste stream (29) is conditioned (122) by mixing with an aqueous medium (39) to form an aqueous suspension of pre-treated bio-waste (30). When the impregnation is with acid, the aqueous medium (39) is a cooled base, such as aqueous ammonia. When the impregnation is based, the aqueous medium (39) is a cooled aqueous stream or a cooled acid, such as sulfuric acid. The pretreated bio-residue suspension (30) is contacted with an enzyme source comprising at least one cellulase in an enzymatic hydrolysis vessel (123) to form an enzyme hydrolyzate suspension (31) comprising monosaccharide sugars. A stream (37) comprising non-fermentable material is extracted from the contents of the enzymatic hydrolysis vessel (123) during or after hydrolysis by means of a non-fermentable material removal apparatus
(125) representative of one or more unit separation operations. The non-fermentable material removal apparatus (125) may be integrated or external to the enzymatic hydrolysis vessel (123) and more than one non-fermentable material removal apparatus may be used. For example, one or more of (i) a foam eliminator can be used to remove the non-fermentable material disposed as a floating layer on the contents of the enzymatic hydrolysis vessel, (ii) a sieve, sieve or filter located in a recirculation circuit or at the outlet to remove the non-fermentable material in suspension from the contents of the hydrolysis vessel
Enzymatic and / or (iii) a gutter or trapdoor for waste to remove the non-fermentable material disposed as a bottom layer in the enzymatic hydrolysis vessel for the removal of the non-fermentable material. The enzyme hydrolyzate suspension
(31) can be combined with a yeast source in a fermentation vessel
(124) to form a fermentation mixture (33) comprising ethanol. One time
After the non-fermentable material has been removed, the hydrolyzate (32) can be combined with a yeast source in a fermentation vessel (124) to form a fermentative mixture (33) comprising ethanol.

权利要求:
Claims (15)
[1]
1. Procedure for preparing monosaccharide sugars from municipal solid waste comprising the following steps: a) optionally remove bulky materials; b) separating urban solid waste by means of a sieve of openings between 60 mm and 100 mm in a first sinking current (4) and a first through current (5), c) separating the first through current (5) from the stage (b) by at least one sieve of openings between 170 mm and 380 mm in a second sinking current (6) and a second through current (7), d) separating the second sinking current (6) from the stage ( c) by means of a ballistic separator in the following currents:
- a stream (8) comprising the fine material with a diameter smaller than the size of the sieve opening of step (b);
- a stream (9) comprising the rolling stock; Y
-  a stream (10) comprising the planar material; e) remove ferrous metal materials from the first sink current (4) of step (b); f) remove non-ferrous metal materials from the current obtained in step (e) g) pass the current obtained in step (f) (11) through a sieve of openings of 5 to 20 mm to form the third sinking current (13) and a third through current (12); h) make a densimetric separation of the third through current (12) of the stage
(g) to form a first light stream (14) and a first dense stream (15);i) separating the first light current (14) from step (h) by a sieve ofopenings between 25 and 50 mm to form a fourth sinking current (16) and afourth through current (17);j) eliminate recyclable materials from the fourth through current (17) of step (i)by an optical separator to form a current (19);k) remove glass, stones and other inert elements from the fourth current ofsunk (16) of stage (i);l) reduce the particle size of the streams obtained in steps (j) and (k) toforming a bio-waste stream (20);

m) recovering the metals present in the stream (9) comprising rolling stock of step (d) to obtain a stream of recyclable material (21) free of metals; n) impregnate the bio-waste stream (20) of step (l) with water, acid or base to form a stream of impregnated bio-waste (28);
5 o) subject the impregnated bio-waste stream (28) of step (n) to a temperature between 100 ° C and 250 ° C and a pressure between 100 kPa and 4,000 kPa for a time between 1 and 20 minutes; and subsequently reduce the pressure to less than 35 kPa to form the pretreated bio-waste stream (29); p) add water, acid or base to the pretreated bio-waste stream (29) of the stage
10 (o) to form a pretreated bio-waste suspension (30); and q) contacting the stream of the pretreated bio-waste suspension (30) of step (p) with an enzymatic composition comprising at least one cellulase enzyme to form an enzyme hydrolyzate suspension (31) comprising monosaccharide sugars and non-material fermentable, where the non-fermentable material
15 is removed from the enzyme hydrolyzate suspension during or after enzymatic hydrolysis.
[2]
2. Method according to claim 1, wherein glass is recovered in a separator
optical from the third sinking current (13) of stage (g) and / or current 20 (22) comprising the materials removed in step (k).
[3]
3. Method according to claims 1 or 2, wherein the second through current
(7) obtained in step (c), after going through a manual classification, it is passed through a densimetric separation to form a second light current (26) and a
25 second dense (27); and where the second light stream is crushed to give rise to a solid fuel.
[4]
4. Method according to claim 1, wherein the enzymatic composition of the
step (q) also comprises at least one hemicellulase. 30
[5]
5. Method according to any one of claims 1 to 4, wherein in step (n) the bio-waste stream (20) is impregnated with acid and in step (p) an aqueous solution of ammonia is added, resulting in a pre-treated bio-residue ( 30) with a solids content between 15-30% by weight, a pH between 4 and 6 and a
35 temperature between 30ºC and 70ºC.

[6]
6. The method according to claim 5, wherein the acid is an inorganic acid and the pretreated bio-waste stream (30) comprises 0.01 to 0.15 kg of acid per kg of solid-base bio-residue.
[7]
7. Method according to any of claims 1 to 4, wherein in step (n) the bio-waste stream (20) is impregnated with base and in step (p) an acid solution is added, resulting in a pre-treated bio-residue (30) with a solids content of between 20% and 30% by weight, a pH between 4 and 6 and a temperature between 30 ° C and
 10 70 ° C.
[8]
8. A method according to claim 7, wherein the base is ammonia and the pretreated bio-waste stream (30) comprises 0.1 to 2.5 kg of ammonia per kg of solid-base bio-residue.
[9]
9. Method according to any one of claims 1 to 8, wherein the enzyme hydrolyzate suspension stream (31) of step (q), during or after hydrolysis, is dehydrated to form:
-  a second stream (37) of impregnated bio-waste having a solids content of between 30% and 70% by weight; Y
- an aqueous stream (32);where at least a part of the aqueous stream is returned to step (q) offormation of the enzyme hydrolyzate suspension stream (31).
Method according to claim 9, wherein the second stream of bio-wastes (37) has a solids content of between 40% and 60% by weight.
[11]
11. Method according to claim 9, wherein the aqueous stream (32) is
It concentrates to form a syrup rich in monosaccharide sugars with a content of 30 monosaccharide sugars of more than 25% of total sugars.
[12]
12. The method according to claim 9, wherein a yeast is added to the aqueous stream (32) or to the enzyme hydrolyzate suspension stream (31) of step (q) to transform the sugars into an organic compound selected from
35 between alcohols or organic acids.

[13]
13. Method according to claim 12 wherein the yeast is Saccharomyces cerevisiae.
14. Process according to any of claims 12 or 13, wherein the organic compound is ethanol.
[15]
15. Method according to any of claims 1 to 14, wherein in the step
(o) the impregnated bio-waste stream (28) is brought into contact with water vapor at a temperature between 150 ° C and 250 ° C and at a pressure between 400 kPa and
 1,750 kPa for a time between 1 minute to 5 minutes; Y
-  the pressure is reduced to a value between 1 to 35 kPa in a single stage; or
-  the pressure is reduced to a pressure of between 40% to 60% in a first stage of pressure reduction; said pressure is maintained for a period of time of
15 between 0.5 minutes to 20 minutes and subsequently the pressure is reduced to between 1 to 35 kPa in a second stage of pressure reduction.
[16]
16. Method according to claim 15, wherein the water vapor has a
temperature between 150ºC and 220ºC and a pressure between 625 KPa and 1,450 kPa. twenty
[17]
17. Method according to any of claims 1 to 16, wherein in the step
(a) prior to step (b) the bulky materials are removed by manual or mechanical classification.

Fig. 1

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同族专利:

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公开号 | 公开日

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ES2688105B1|2019-09-16|

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EP3385196A1|2018-10-10|

引用文献:

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公开号 | 申请日 | 公开日 | 申请人 | 专利标题

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DE20018754U1|2000-11-02|2000-12-28|Kimmlinger Karl|bag|

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DE202004007682U1|2004-05-13|2004-08-26|Handeck, Claus G.|Kitchen towel, serviette or tissue is made from cellulosic material and contains aroma-producing material|

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ES1139519Y|2015-02-17|2015-08-19|Munoz Jovito Perez|Garbage bag|

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EP17382576.1A| EP3385196A1|2017-04-07|2017-08-18|Trash bag|