• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    The present invention relates to a microdiet to feed common octopus paralarvae in order to obtain benthic juveniles. The microdiet includes different components: freeze-dried squid meal, egg yolk powder, oils of marine origin and a mixture of micro-elements (fe, mg, cu and s). The microdiet presents a particle size of 250-1000 μm; and was supplied from day 30-40 of culture in 3 - 18 intakes/daily. The microdiet was accepted and ingested by the paralarvae, inducing positive growth until 73 days of age. (Machine-translation by Google Translate, not legally binding)
    公开号:ES2599603A1
    申请号:ES201500582
    申请日:2015-07-28
    公开日:2017-02-02
    发明作者:Juan A. ESTEFANELL UCHA；Antonio MESA RODRÍGUEZ；María Soledad IZQUIERDO LÓPEZ；Besay RAMÍREZ BORDÓN；Juan SOCORRO CRUZ
    申请人:Universidad de las Palmas de Gran Canaria；
    IPC主号:
    专利说明:


    Micro octopus for octopus paralarvas of common octopus vulgaris (Cuvier, 1797)
    II. SECTOR OF THE TECHNIQUE
    The present invention falls within the "Animal production" sector, specifically within the scientific-technical area of "aquaculture", and corresponds to a specific formulated diet for initial larval stages of common octopus, generating a high intake and rates Positive growth
    10 III. STATE OF THE TECHNIQUE
    The common octopus Octopus vulgaris is of great importance as a fishery resource in many countries of the Mediterranean, Central America and Asia. The over-exploitation of its fishery has dramatically decreased catches (FAO, 2012), increasing its market value and demand (Vaz Pires et al. 2004). For this reason, since the late 1990s there has been a growing interest in developing cultivation technology for this species (Iglesias et al. 2000). However, despite its good biological yields observed in the fattening phase (Socorro et al. 2005; Estefanell et al. 2012), octopus culture is limited by low survival in the larval phase, which prevents its industrial scale cultivation (Iglesias et al. 2007).
    20 The larval culture protocols of marine fish, the commonly used live prey as well as the commercially used enrichers and micro-diets have not produced good results in the larval culture of O. vulgaris (Navarro and Villanueva, 2003; Seixas et al. 2010; Okumura et al. 2005) or in other species of Merobentonic cephalopods such as Octopus mimus or Robsonella fontaniana (Uriarte et al. 2011). 25 Paralarvas, despite having an initial pelagic phase similar to fish larvae, have specific nutritional and zootechnical requirements.
    At the experimental level, different experimental enrichers and micro-diets have been tested, without ever obtaining benthic juveniles (Navarro and Villanueva, 2003; Iglesias et al. 2007; Estefanell et al. 2013). In fact, the few 30 published studies where a few benthic specimens were obtained used exclusively live food as a diet, from natural zooplankton (Villanueva, 1995; Iglesias et al. 2004; Carrasco et al. 2006). However, these protocols are not applicable on an industrial scale, since it requires the capture and collection of wild zooplankton, presenting several drawbacks; It is expensive, has a low yield and goes 35 against the sustainability of marine ecosystems.
    In general to date the results published with micro-diets for paralarvae of
    Octopus have not given good results. However, there is information available on a JACUMAR project “Nutrition and feeding of paralarvas and subadults of rock octopus” (2010-2013) in which two micro-diets were tested in 30-day-old paralarvas. The experiment was carried out in the facilities of the IEO of Vigo, lasted 5 of 9 days, and 2 feeding protocols were tested, both based on microdiet supplemented with 2 intakes of 0.5 artemia / mL. One of the micro-diets was commercial and another experimental (formulated by our research group). The growth was marginal, of only 0.1 mg in dry weight in the 9 days, suggesting that it was due to enriched artemia.
    10 * On the other hand, the use of a formulated dry diet has several advantages over fresh food: it allows a standardization of the diet regardless of seasonal variations, easy conservation and transport, a low risk of disease transmission and a lower impact environmental (Lee, 1994). In addition, it is an essential step to study the nutritional requirements of a new species for
    15 aquaculture, since it allows incorporating different components and raw materials.
    The present invention presents a formulated microdiet that is accepted and ingested by common octopus paralarvas, for the first time inducing a significant weight gain throughout the crop.
    20 References
    • Carrasco, J.F., Arronte, J.C., Rodríguez, C. 2006. Paralarval rearing of the common octopus, Octopus vulgaris (Cuvier). Aquaculture Research 37, 1601-1605.
    • Estefanell J., Roo J., Guirao R., Izquierdo M., Socorro J. 2012. Benthic cages versus floating cages ¡n Octopus vulgaris: biological performance and biochemical
    25 composition feeding on Boops boops discarded from fish farms. Aquacultural Engineering, 49, 46-52.
    • Estefanell, J., Biger B., Socorro, J., Izquierdo, M., Roo J. 2013. Growth, survival and histology of the digestive gland in paralarvae of Octopus vulgaris fed on Artemia enriched on EPA, DHA and 5 levels of ARA. Aquaculture Conference: to the next 40
    30 years of sustainable global aquaculture. Book of abstracts. Las Palmas (Spain).
    • Iglesias, J., Otero, J.J., Moxica, C., Fuentes, L., Sánchez, F.J. 2004. The completed life cycle of the octopus (Octopus vulgaris, Cuvier) under culture conditions: paralarvae rearing using Artemiaanó zoeae, and first data on juvenile growth up to eight months of age. Aquaculture International 12, 481-487.
    35 • Iglesias, J., Sánchez, F.J., Bersano, J.G. F., Carrasco, J.F., Dhont, J., Fuentes, L.,
    5
    10
    fifteen
    twenty
    25
    30
    Linares, F., Muñoz, J.L., Okumura, S., Roo, J., Van Der Meeren, T., Vidal, E.A.G., Villanueva, R. 2007a. Rearing of Octopus vulgaris paralarvae: present status, bottlenecks and trends. Aquaculture 266, 1-15.
    • Iglesias, J., Sánchez, F.J., Otero, J.J., Moxica, C. 2000. Culture of octopus (Octopus vulgaris, Cuvier): present, knowledge, problems and perspectives. Recent Advances in Mediterranean Aquaculture Finfish Species Diversification Cahiers Options Méditerranéennes 47, 313-322.
    • Read P.G. (1994) Metabolic substrates in cephalopods. In: Physiology of Cephalopod Molluscs, Lifestyle and Performance Adaptations (ed. By H.O. Portner, R.K. 0’Dor & D.L. MacMillan), pp. 35-51. Gordon & Breach, Switzerland.
    • Navarro, J.C., Villanueva, R. 2003. The fatty acid composition of Octopus vulgaris paralarvae reared with live and inert food: deviation from their natural fatty acid profile. Aquaculture 219, 613-631.
    • Okumura, S., Kurihara, A., Iwamoto, A. Takeuchi, T. 2005. Improved survival and growth in Octopus vulgaris paralarvae by feeding large type Artemia and Pacific sandeel, Ammodytes personatus: Improved survival and growth of common octopus paralarvae. Aquaculture 24, 147-157.
    • Seixas, P., Otero, A., Valente, L.M.P., Dias, J., Rey-Méndez, M. 2010. Growth and fatty acid composition of Octopus vulgaris paralarvae fed with enriched Artemia or co-fed with an inert diet. Aquaculture International 18 (6), 1121-1135.
    • Socorro, J., Roo, J., Fernández-López, A., Guirao, R., Reyes, T., Izquierdo, M. 2005. Ongrowing of Octopus vulgaris (Cuvier, 1797) in floating cages fed with bogue Boops boops (L., 1758) from fish farm discards. Bulletin of the Spanish Institute of Oceanography 21 (1-4), 189-194.
    • Uñarte, I., Iglesias, J., Domingues, P., Rosas, C., Viana, MT, Navarro, JC, Seixas, P., Vidal, E., Ausburger, A., Pereda, S., Godoy , F., Paschke, K., Farías, A., Olivares, A., Zúñiga, O. 2011. Current status and bottleneck of octopod aquaculture: the case of American species. Journal of the World Aquaculture Society 42 (6), 735-752.
    • Vaz-Pires, P., Seixas, P., Barbosa, A. 2004. Aquaculture potential of the common octopus (Octopus vulgaris Cuvier, 1797): a review. Aquaculture 238, 221-238.
    • Villanueva, R. 1995. Experimental rearing and growth of planktonic Octopus vulgaris from hatching to settlement. Canadian Journal of Fisheries and Aquatic Sciences 52, 2639-2650
    IV. SUMMARY. DESCRIPTION OF THE INVENTION
    The present invention is the first specific micropod for common octopus that has given positive results. The micro-diet includes different components: freeze-dried squid flour, egg yolk powder, marine oils and a mixture of micro elements (Fe, Mg, Cu and S). The microdiet has a particle size of 250-1000 p and was supplied from day 30-40 of culture in 3-18 shots / day.
    IV-A. Detailed description of the invention
    10 The microdiet contains the following raw materials:
    • Freeze-dried squid flour: based on fresh squid of 20-30 g. The content was 60-70%.
    • Egg powder (Whole hen egg powder, Immaculate Eggs, Murcia, Spain). The content was 10-15%
    15 • Vevodar Oil, DSM Nutritional Products (Basel, Switzerland). The content was 2-6%
    • Krill oil (Qrilloil TM, AkerBioMarine ASA, Oslo, Norway). The content was 2-4%
    • Gelatin 80-100 Blooms, PRS-CODEX (Panreac-142060.1210, E.U). The content was 4-8%
    20 • Organic Minerals: iron, manganese, sulfur and copper were added (Alltech’s
    Bioplex, USA), in amounts of at least 0.1-0.5%.
    • Vitamin E: DL-a-Tocopherol acétate (Sigma-Aldrich Co., MO, USA), in amounts of
    0. 5.2%
    25 Detailed elaboration protocol: The preparation of the microdiet is carried out following the following steps:
    1. Weigh, grind and mix the different dry raw materials to be used (squid flour, organic minerals and egg powder).
    2. Weigh and mix with different stirrer the different fluid raw materials (Krill oil, 30 Vevodar oil and Vit. E).
    3. Unify and homogenize all the aforementioned raw materials.
    4. Weigh the gelatin and dilute in boiled distilled water. Once its temperature has been reduced to 36 degrees, add to the mixture.
    5. Pressing and drying (Stove at 37 degrees) for 24 hours.
    35 6. Mechanical grinding and sieving of the microdiet to obtain the desired size.
    5
    10
    fifteen
    twenty

    Feeding protocol: The paralarvas were cultured with artemia up to 30-40 days. From there, a weaning protocol was applied, combining the incorporation of 3 initial micro-diet shots up to 18 daily doses at day 55 of culture (weaning phase). After 55 days, she was fed only with a microdiet until the paralarvas became benthic at 73 days. The initial particle size was 250-500 microns and at the end 700-1000 microns.
    IV-B Brief Description of the Invention
    The present invention relates to a micropowder for common octopus paralarvas, which has been ingested by the larvae generating positive growth, both during weaning and afterwards. It is the first time that a micro-diet for common octopus paralarvas Octopus vulgaris is developed.
    V. DETAILED DESCRIPTION OF A PREFERRED EMBODIMENT OF THE INVENTION
    Example 1: acceptance test of the micro-diet in co-feeding regime with artemia. 40-day-old paralarvas (dry weight = 0.82-1.06 mg) were fed the experimental micro-diet based on 3 daily intakes of 1 particle per paralarva and 2 million artemia distributed in 8 intakes. The number of microdiet shots was gradually increased to 16 distributed throughout the 12 hours of light, while the amount of artemia was reduced, to 3 shots of 100,000 artemias each day 56 of cultivation (weight dry = 1.94 mg). At this point the micro-diet was suppressed, and the paralarvas were grown up to 73 days based exclusively on the micro-diet, at which time they began to have a benthic behavior (dry weight = 2.53 mg).
    权利要求:
    Claims (6)
    [1]
    1. A specific formulated microdiet for common octopus paralarvas. The micro-diet includes different components: freeze-dried squid flour, 5 egg yolk powder, marine oils, vitamin E and a micro mix
    elements (Fe, Mg, Cu and S). The microdiet has a particle size of 250-1000 p and was supplied from day 30-40 of culture in 3-18 shots / day
    2. Microdiet according to claim 1, containing lyophilized squid flour,
    Powdered egg yolk, marine oils and microelements.
    fifteen
    [3]
    3. Micro-diet according to claim 1, wherein there is lyophilized squid flour and is represented between 60-70% of the formula.
    [4]
    4. Microdiet according to claim 1, wherein there is egg powder and is represented between 10-15% of the formula
    twenty
    [5]
    5. Micro-diet according to claim 1, wherein there is Vevodar Oil and is represented between 2-6% of the formula
    [6]
    6. Micro-diet according to claim 1, wherein there is Krill oil and is represented between 2-4% of the formula
    7. Microdiet according to claim 1, wherein there are organic minerals (iron,
    manganese, sulfur and copper) and are represented between 0.1-0.5% of the formula
    30
    [8]
    8. Micro-diet according to claim 1, wherein there is Vitamin E and is represented between 0.5-2% of the formula
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    同族专利:
    公开号 | 公开日
    ES2599603B2|2018-05-14|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    FR2572625A1|1984-11-07|1986-05-09|Univ Languedoc|Compound food for aquatic animals and methods and devices for raising fish larvae|
    US6645536B2|2001-03-29|2003-11-11|Mississippi State University|Micro-particulate microbound diet for the culture of larval fish and crustaceans|
    WO2008084074A2|2007-01-10|2008-07-17|Blue Limit As|Feed composition for aquatic organisms|CN107927459A|2017-12-14|2018-04-20|浙江海洋大学|A kind of soft pellet diet formula and application suitable for the cultivation of octopus class|
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