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  • 专利说明
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    • 专利摘要:
    Method for the determination of the racial purity of a specimen of porcine species and/or products derived from it. The present invention relates to a method for determining the racial purity of a specimen of porcine species and/or products derived therefrom by detecting in a sample obtained from the animal or product to be analyzed, from at least one selected nuclear snp. Between located at positions 1318 of the mc1r gene and 3072 the igf2 gene and of the at least one mitochondrial snp selected from 4777 of the nd1 gene, 10672 of the coa gene and 16279 of the cyb gene. (Machine-translation by Google Translate, not legally binding)
    公开号:ES2598907A1
    申请号:ES201531118
    申请日:2015-07-28
    公开日:2017-01-30
    发明作者:Alberto MEMBRILLO DEL POZO；Antonio MOLINA ALCALÁ；Gabriel DORADO PÉREZ；Ignacio De Loyola CLEMENTE LÓPEZ；Antonio RODERO FRANGANILLO
    申请人:Universidad de Cordoba；
    IPC主号:
    专利说明:

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    Due to the implication of this gene and specifically the SNP described in the metabolism, it
    selected as polymorphism to be used in this invention. The study of this SNP molecular marker was performed by allele-specific real-time PCR using the methodology, primer sequences and probes described by
    5 Carrodeguas et al. (2005). Allelic frequencies of this point in each of the races are shown in Table 2. Primers: IGF2F: SEQ ID NO: 1 IGF2R: SEQ ID NO: 2 10 Taqman probes: IGF2V1: SEQ ID NO: 3, marked with VIC IGF2M1 fluorophore: SEQ ID NO: 4, labeled with FAM fluorophore
    Table 1. Frequencies of the polymorphism detected in position c. 3072 G> A of the 15 igf2 gene in the different pig races analyzed NUCLEOTETIC FREQUENCIES
    POSITION FRAGMENT POLYMORPHISM IBEHardMANIND
    3072 IGF2TO0oneoneone
    G one000
    DUR: Duroc; IBE: Iberian; IND: Industrial crossing; MAN: Mangalitza.
    As can be seen, the G allele is exclusively fixed in the Iberian population, while in the rest of the races, the allele that is fixed is the A allele. The relationship of this gene with the greater or lesser lean growth (Ruiz et al., 2002; Carrodeguas et al., 20 2005) explain the fact that one allele (A) is fixed in the Iberian pig while the other (G) does it in the Duroc. This means that we have considered this marker of extreme interest for our objectives (especially when we want to certify racial purity). However, given that the norm allows the crossing with Duroc (as long as it does not occur by the mother or in a proportion greater than 50%) it is necessary to include other
    25 molecular markers.
    10

    Melanocortin receptor 1 gene (mc1r)
    The gene encoding the melanocortin receptor (mc1r) is part of the main known regulatory system of pigment synthesis, together with the coupled G protein and protein
    5 agoutí. The binding of the melanocyte stimulating hormone (MSH) to the MC1R initiates the signal that is transduced through the enzyme tyrosinase into the cellular interior, which leads to the synthesis of eumelanin (black-brown pigment).
    Of all the polymorphic positions detected (positions 603, 608, 668, 759, 802, 878, 896, 1110, 1197, 1318, and 1154), the SNPs that have the greatest interest for our objectives are the
    10 located in positions 1318 and 1554 (described by Kijas el al., 1998), referring to the sequence with GenBank accession number AF326520 that are fixed in the Duroc breed and that make up the MC1R * 4 haplotype. Position 1318 (Kijas et al., 1998) has been selected as a detection polymorphism of the Duroc breed, since, because of the proximity between the two points, it is unlikely that they will segregate independently.
    The study of this SNP molecular marker was performed by allele-specific real-time PCR using the methodology and sequences of Taqman primers and probes designed by the authors of the present invention.
    Primers:
    PigMc1r-1F: SEQ ID NO: 5
    20 PigMc1r-1R: SEQ ID NO: 6 Taqman probes:
    Mc1rPig1C.-SEQ ID NO: 7, marked with FAM fluorophore
    Mc1rPig1T: SEQ ID NO: 8, marked with HEX fluorophore
    Table 2. Frequencies of the polymorphism detected in position c. 1318 C> T of the mc1r gene in the different pig races analyzed.
    25 NUCLEOTIDIC FREQUENCY
    POSITION FRAGMENTPOLYMORPHISMIBEHardMANIND
    1318 MC1R4Cone0oneone
    T 0one0^ 0
    DUR: Duroc; IBE: Iberian; IND: Industrial crossing; MAN: Mangalitza
    eleven
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    权利要求:
    Claims (1)
    [1]
    image 1
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    同族专利:
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    引用文献:
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    优先权:
    申请号 | 申请日 | 专利标题
    ES201531118A|ES2598907B2|2015-07-28|2015-07-28|METHOD FOR THE DETERMINATION OF RACIAL PURITY OF AN EXAMPLE OF SWINE SPECIES AND / OR PRODUCTS DERIVED FROM THE SAME|ES201531118A| ES2598907B2|2015-07-28|2015-07-28|METHOD FOR THE DETERMINATION OF RACIAL PURITY OF AN EXAMPLE OF SWINE SPECIES AND / OR PRODUCTS DERIVED FROM THE SAME|
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