• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    Device method for analysis of biological material, method of obtaining and using it. A method of manufacturing a detection device for biological material is described in this document, a device that presents a series of micromotors that can be selectively functionalized and guided inside a microfluidic device in which they are inserted in such a way that they can be carry out detection of those analytes that are required quickly and effectively. The device makes use of autonomous nanomotors that are manufactured by various deposition and/or sputtering techniques. (Machine-translation by Google Translate, not legally binding)
    公开号:ES2598878A1
    申请号:ES201531139
    申请日:2015-07-30
    公开日:2017-01-30
    发明作者:Sergio MUÑOZ LEZCANO；Miguel Ángel ARMENGOL DE LA HOZ；Esther ALEO LUJÁN；Luis ARRUZA GÓMEZ；Ana ARRIBI VILELA；Alberto ESCARPA MIGUEL；Cristina GONZÁLEZ MARTÍN；Miguel A. LÓPEZ GIL；Aída MARTÍN GALÁN；Beatriz JURADO SÁNCHEZ
    申请人:Universidad de Alcala de Henares UAH；Fundacion para la Investigacion Biomedica del Hospital Clinico San Carlos；
    IPC主号:
    专利说明:

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    magnetic.
    Another possible embodiment of the third aspect of the invention is electrochemical detection, which is carried out very similarly to the above-described embodiment of the third aspect of the invention in which the molecules used for its detection (QDs) are replaced by nanoparticles / nanobars ("nanorods") of different metals, as many as analytes are determined. In this sense, the specific secondary Ab of each analyte are co-immobilized in the particles (silica mesoporoses) together with nanoparticles of a certain metal.
    The detection will occur after the release of the different metal nanoparticles (a different metal for each analyte) and whose amount will be given by the concentration of analyte in the sample. This detection is carried out by means of an electrochemical technique of redisolution on the surface of an electrode included in the third well (223) or detection well.
    In a last possible embodiment of the third aspect of the invention there is an enzymatic determination, which is carried out in a manner similar to the previous embodiments of the third aspect of the invention, whose fundamental difference lies in the independent determination of the different biomarkers. This will be carried out in a single microchip that incorporates the channels (21) and their corresponding wells (221,222,223), as parallel systems for the independent and multiplexed determination of the different analytes.
    Therefore, the steps of the procedure are repeated for each of the systems dedicated to a certain analyte and all of them included in the same device.
    one. Now, in the first well (221) a single population of micromotors (5) specific for that particular analyte is added, together with the sample volume and the fuel for its movement.
    2. Sections 2 and 4 remain the same as in the previous procedures.
    3. The secondary Ab (specific for each analyte and in its corresponding system) are co-immobilized together with numerous enzyme molecules
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    contains contains specific secondary molecules of the analyte, said orientation can be done by magnetic induction and guided by bubble or ultrasound propulsion.
    - perform an orientation and guidance of the micromotor (5) from the second well
    5 (222) to a third well (223) of the device, called a detection well in which a detection of analyte concentration present in the sample occurs as in the previous step, the orientation can be done by magnetic induction and guided by bubble or ultrasound propulsion.
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    fifteen
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    权利要求:
    Claims (1)
    [1]
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    同族专利:
    公开号 | 公开日
    ES2598878B1|2017-10-24|
    WO2017017309A1|2017-02-02|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    WO2012166215A2|2011-06-02|2012-12-06|The Regents Of The University Of California|Membrane template synthesis of microtube engines|CN107584112B|2017-09-04|2019-08-09|哈尔滨工业大学|A kind of preparation method of gallium nanometer rods and its application as nano-motor|
    法律状态:
    2017-10-24| FG2A| Definitive protection|Ref document number: 2598878 Country of ref document: ES Kind code of ref document: B1 Effective date: 20171024 |
    优先权:
    申请号 | 申请日 | 专利标题
    ES201531139A|ES2598878B1|2015-07-30|2015-07-30|METHOD AND DEVICE FOR ANALYSIS OF BIOLOGICAL MATERIAL, METHOD OF OBTAINING AND USING THE SAME|ES201531139A| ES2598878B1|2015-07-30|2015-07-30|METHOD AND DEVICE FOR ANALYSIS OF BIOLOGICAL MATERIAL, METHOD OF OBTAINING AND USING THE SAME|
    PCT/ES2016/070583| WO2017017309A1|2015-07-30|2016-07-29|Method of analysing biological material and a method of manufacturing a microfluidic device comprising micromotors|
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