• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    The invention relates to a container (1) for filtering a suspension (16) which comprises a cover (3) and a vessel (2). The container (1) has a filter (12) which divides an interior (4) of the container (1) into a first compartment (13) and a second compartment (14). The cover (3) has a first access (7) and a second access (8). The first access (7) is connected to the first compartment (13) and the second access (8) is connected to the second compartment (14). The invention also relates to a method for filtering a suspension with this container.
    公开号:CH713044B1
    申请号:CH00213/18
    申请日:2016-08-25
    公开日:2020-10-30
    发明作者:Wilhelm Bernd-Ulrich；Klattkowsky Ulrike；Millauer Jan
    申请人:Bbi Biotech Gmbh；
    IPC主号:
    专利说明:

    The invention relates to a container for filtering a suspension according to the preamble of claim 1. The invention also relates to a method for receiving and filtering a suspension.
    [0002] Containers that allow a suspension to be taken up and filtered are known from the prior art. For example, US Pat. No. 4,897,193 shows a device which consists of a mixing container into which a mixer-separator is fitted in a flush and movable manner. The mixer-separator has a selective barrier at a lower end and a channel in a vertical axis communicating therewith. This device can be used to filter a suspension in the mixing container. The mixer-separator is pressed into the mixing container. This pushes the suspension through the selective barrier so that a filtrate rises into the channel.
    It is the object of the present invention to propose a container which is suitable for filtering a suspension. The invention is also based on the object of proposing a corresponding method for filtering a suspension.
    This object is achieved by a container with the features of the main claim and by a method with the features of the independent claim. Advantageous developments result from the features of the dependent claims and the exemplary embodiment.
    The proposed container comprises a lid and a vessel. The container has a filter which divides an interior space of the container into a first compartment and a second compartment. The cover has a first access and a second access. The first access is connected to the first compartment and the second access to the second compartment.
    The accesses are typically also referred to as ports. The fact that the container has a first and a second access means that a suspension can be filled in and removed through different accesses without the lid of the container having to be removed from the vessel in order to fill in a suspension or some other liquid. In contrast to known containers for the filtration of suspensions, which require the container to be opened to fill the suspension, this avoids the entry of undesired particles, gases or liquids into the interior during filling. In addition, it is avoided that during the filling process germs capable of replication from the interior of the container get into the surroundings of the container and vice versa from the surroundings of the container into the interior of the container. In this way, sterile conditions can be ensured during filling.
    The filter exhibits selective permeability and is typically arranged to prevent suspended solids from flowing through. As a rule, the filter is permeable for particles up to a certain size, while it blocks a flow for particles above this certain size.
    If, for example, a liquid is filled into the interior space through the first access, the suspension is initially in the first compartment. Since the first compartment and the second compartment are separated from one another by the filter, removal of the liquid from the second compartment through the second access requires the liquid to flow through the filter. If the liquid filled in is a suspension with a certain mean particle size, with a suitable filter it can be achieved that the removed liquid is a filtrate with a smaller mean particle size or without particles.
    The proposed container has a simple structure. In addition, the filtration does not necessarily take place through a mechanical displacement of parts of the container relative to one another. In many embodiments, however, a mechanical displacement can be dispensed with. Use of the proposed container is particularly advantageous for automated filtration of a suspension by a machine, because filtration by mechanically displacing parts of the container relative to one another would require the machine to have a more expensive or complex structure, which leads to increased wear the machine.
    The container also allows a suspension or other liquid to be stored and transported. A suspension can also be filtered during its storage or during its transport. In addition, no additional filtering device, such as a centrifuge, into which the suspension or other liquid has to be transferred from a transport container for the filtration, is required when using the proposed container.
    The suspension can be, for example, a suspension with cells or microorganisms, such as bacteria or microscopic algae. Typically, such suspensions are cultivated in a bioreactor. Often, before analyzing such a suspension, for example in a high-performance liquid chromatograph, it is necessary to filter the suspension in order to separate cells or cell debris from the liquid. Filtration can also be necessary or advantageous in other analysis methods, for example in cytometry, electrophoresis, mass spectrometry or analysis using a bioarray or biochip.
    [0012] The proposed method for filtering a suspension is accordingly advantageous. In this method, a container comprising a lid and a vessel is first provided. The container has a filter which divides an interior space of the container into a first compartment and a second compartment. The cover has a first access and a second access. The first access is connected to the first compartment and the second access is connected to the second compartment. A liquid to be filtered is then filled into the interior through the first access or through the second access and a filtered liquid is removed from the interior through the second or first access.
    [0013] Filling and removal can take place simultaneously. If the filling and the removal are at the same time, continuous filtration can take place, so that a continuous inflow of a suspension and a continuous outflow of a filtrate take place. However, it is also possible that first the filling and then the removal take place. In this way, a liquid can also be stored between filling and removal. It is also possible that the filling and the removal take place at times simultaneously and at times individually.
    In one embodiment, a pressure difference is applied between the first and the second access in the method. For example, a suspension can be filled into the first compartment by applying increased pressure to the first access and removed from the second compartment by applying a low pressure to the second access. This enables an increased rate of filtration to be achieved. In addition, after the cover has been mounted on the vessel, no further mechanical displacement of the cover relative to the vessel is necessary.
    In one embodiment, the filter is arranged such that the first compartment and the second compartment are each delimited by a part of the vessel, by a part of the lid and by the filter. For example, the filter can be arranged centrally between the first and the second access and extend from the cover to an underside of the vessel.
    In a further embodiment, the filter is arranged such that the first compartment or the second compartment is limited exclusively by a part of the lid and by the filter. In this embodiment, the filter is typically attached to the lid and is not in direct contact with the vessel. For example, it is possible that the filter is arranged directly under the first access, so that filling a suspension through the first access into the first compartment results in the suspension dripping into the second compartment as a result of the force of gravity through the filter, and is filtered during the passage through the filter, so that a filtrate collects on a bottom of the vessel belonging to the second compartment.
    The filter can be a membrane. Depending on the type of liquid to be filtered, the membrane can comprise, for example, metal, ceramic, carbon, cellulose, glass microfibers, nylon, polypropylene, polytetrafluoroethylene, polyethersulfone or polyvinylidene fluoride or a combination of the aforementioned materials. In addition, the membrane can be designed as a hollow fiber membrane.
    [0018] The filter typically has pores with a diameter of at least 0.02 μm and / or at most 10 μm, preferably of at least 0.5 μm and / or at most 3 μm. The pore size determines the size of the particles filtered out of the suspension. A pore size between 0.02 µm and 10 µm is suitable for the filtration of a large number of different suspensions that contain microorganisms or cells. A size of bacteria is typically 1 to 10 µm. If, for example, a filter with pores with a diameter of 0.9 µm is used, bacteria present in a suspension can usually be filtered out of it.
    The interior typically has a volume of at least 0.1 ml and / or at most 50 ml. Such a volume of the interior is suitable for quantities of liquid that are typically to be filtered, transported and stored in laboratories. In particular, the volume is suitable for amounts of liquid that are typically withdrawn from a bioreactor in order to analyze processes taking place in the bioreactor and the conditions prevailing therein.
    [0020] The vessel can be closed in a sterile manner by the lid. Thus, the cover can prevent germs capable of replication from penetrating into the interior space as well as germs capable of replication from the interior space in the environment. In addition, contamination of samples or suspensions located in the container can thus be counteracted.
    The lid and the vessel can for example be screwed or otherwise connected to one another. It is also possible for the lid and the vessel to be designed to be connected. For example, the lid and the vessel can be made in one piece.
    In addition, the container can be packaged sterile before use.
    The vessel and / or the lid typically comprises a thermoplastic material. The vessel and / or the lid is preferably made essentially from a thermoplastic material. Typically, both the vessel and the lid are made of polypropylene. However, it is also possible for the vessel and / or the lid to be made from a thermoset or from a metal. The vessel can also be made from a plastic film, so that it is easily deformable and can be stored in a space-saving manner. By using inexpensive materials that are easy to process, it can be achieved that the container is suitable for a single use. A one-time use, that is, a use of the container as a disposable item, saves a costly and energy-intensive cleaning step, which may be necessary in particular between using the container with different suspensions containing microorganisms or cells.
    The first and / or the second access typically has a septum. The suspension can be filled in and / or removed, for example, by means of a syringe needle. Accesses to the interior can in particular be designed as ports with septa. The septa are typically designed to be pierceable by a pointed needle. In addition, the septa can typically reclose automatically after a syringe needle is withdrawn. The septa can be made of polypropylene or silicone, for example. The septa can be made of an elastomer.
    The vessel typically has a substantially cylindrical shape. Likewise, the lid typically has a substantially cylindrical shape, so that the container has a substantially cylindrical shape. A cylindrical shape and a certain size of the vessel can be provided so that the container fits as closely as possible in receptacles typically located on mechanical shaking devices, autosamplers or other feeding devices. In addition, a cylindrical shape is also suitable in that the relative orientation of the lid to the vessel does not have to be taken into account when the vessel is closed or opened. Alternatively, the vessel and the lid can also have an essentially oval cross section. A conical vessel is also possible.
    The vessel typically has a rounded or flat bottom. In addition, the vessel typically has a height that is greater than a cross-section of the vessel.
    In one embodiment, the container has a positioning aid, the positioning aid being set up to guide the container into a receptacle in a fixed orientation. The positioning aid is typically designed in the form of an outwardly directed extension, such as, for example, a pin, of the cover. The fixed alignment can be achieved in that the extension engages in a groove provided on the receptacle. Conversely, a groove can also be provided on the cover, into which an extension provided on the receptacle engages. The positioning aid can also be designed in the form of a flattened side of the cover. In addition, a positioning aid can also be provided on the vessel. This can be designed, for example, in the form of a flattened side of the vessel, a groove or an extension such as a pin. The relative orientation of the lid to the vessel in the case of a closed container can also be determined by a special shape of the lid and the vessel.
    A positioning aid can be advantageous if the accesses are not arranged in a rotationally symmetrical manner in the cover and, for example, are arranged next to one another on the cover. If, for example, an apparatus is set up for the automated removal of liquids from a container located in the receptacle, it can be advantageous if the accesses are always arranged in the same position relative to the receptacle. Appropriate positioning of the accesses can be achieved using the positioning aid.
    The first access and / or the second access is typically suitable for treating a suspension located in the interior with ultrasound. For this purpose, the accesses have a diameter of over 1 mm, for example, in order to allow access with an ultrasonic sonotrode.
    In one embodiment, the container has at least one pressure compensation filter which enables pressure compensation between the first compartment and / or the second compartment and the surroundings of the container. In one embodiment, the pressure compensation filter is provided on the cover. Alternatively, the pressure compensation filter can also be provided on the vessel. Typically, the pressure compensation filter creates a sterile and air or gas-permeable connection between the environment and the interior of the container. For this purpose, the pressure compensation filter can be designed as a membrane and / or have an average pore size of 0.2 μm or less. In particular, the mean pore size of the pressure compensation filter can be between 0.1 and 0.2 μm. In one embodiment, the pressure compensation filter comprises hydrophobic material, so that no large amounts of liquid adhere to it. This ensures that the filter is reliably permeable to air or other gases. A pressure equalization between the interior and the environment can be advantageous when the container is filled with a liquid or when a liquid is removed from the container. In particular, the presence of a pressure compensation filter can enable the container to be filled more quickly.Features of the container or the method can be combined with one another and claimed individually.
    [0031] Embodiments of the invention are described below with reference to the figures. Show it<tb> Fig. 1 <SEP> a cross section of a container according to a first exemplary embodiment,<tb> Fig. 2 <SEP> another cross-section of the container,<tb> Fig. 3 <SEP> a cross section of a container according to a second embodiment,<tb> Fig. 4 <SEP> shows a cross section of a container according to a third exemplary embodiment,<tb> Fig. 5 <SEP> shows a further cross section of the container according to the third exemplary embodiment,<tb> Fig. 6 <SEP> a further cross section of the container according to the first embodiment, a suspension and a filtrate,<tb> Fig. 7 <SEP> a further cross section of the container according to the first exemplary embodiment, a suspension, a filtrate and a first and a second syringe needle,<tb> Fig. 8 <SEP> a top view of the container according to the first embodiment and a recording,<tb> Fig. 9 <SEP> shows a cross section of the container according to a fourth exemplary embodiment,<tb> Fig. 10 <SEP> a top view of the container and a recording,<tb> Fig. 11 <SEP> shows a cross section of a container according to a fifth embodiment,<tb> Fig. 12 <SEP> a side view of a container according to a sixth embodiment,<tb> Fig. 13 <SEP> shows a cross section of a container according to a seventh embodiment,<tb> Fig. 14 <SEP> a plan view of a cover according to a further exemplary embodiment,<tb> Fig. 15 <SEP> shows a cross section of a container in accordance with an eighth exemplary embodiment and<tb> Fig. 16 <SEP> shows a further cross section of the container according to the eighth exemplary embodiment.
    Fig. 1 shows a container 1 in a first embodiment. The container comprises a vessel 2 and a lid 3. The lid 3 and the vessel 2 enclose an interior space 4 of the container 1, the interior space 4 having a volume of approximately 40 ml. The vessel 2 is made of polypropylene and has a flat standing surface 5 on an underside 6. The lid 3 is screwed onto the vessel 2 in a sterile manner and is also made of polypropylene.
    The cover 3 has a first access 7 and a second access 8, which are each designed in the form of recesses in the cover 3 with a round cross section. A first septum 9 made of silicone is located on the first access 7. On the second access 8 there is a second septum 10 made of polypropylene. The cover 3 additionally comprises a positioning aid 11 in the form of an outwardly directed extension.
    A height of the vessel 2 is, for example, 4 cm and a radius of the round base 5 is, for example, 2 cm. The vessel 2 can, for example, have a wall thickness of less than 1 mm.
    In addition, the container comprises a filter 12. The filter 12 runs vertically from the lid 3 to the bottom 6 of the vessel 2 and divides the interior 4 of the container into a first compartment 13 and a second compartment 14. The respective compartments 13, 14 are arranged such that the first compartment 13 is connected to the first access 7 and the second compartment 14 is connected to the second access 8.
    It can also be provided that the filter 12 does not extend from the bottom 6 of the vessel 2 to the lid 3, but in an embodiment with a smaller area of the filter 12 from the bottom 6 of the vessel 2 to a certain height in the vessel 2 extends. In this case, the first compartment 13 and the second compartment 14 are not completely separated from one another by the filter 12, but are instead connected to one another by an opening. In addition, the first access 13 and the second access 14 are then connected to one another, such an embodiment of the vessel 2 being suitable as long as the vessel 2 is only filled with a liquid to a level below the certain level.
    The filter 12 may include, for example, glass microfiber, nylon, polypropylene, polytetrafluoroethylene, polyethersulfone, or polyvinylidene fluoro. It is also possible that the filter 12 is a metallic filter, a ceramic filter, a carbon filter or a cellulose filter.
    2 shows a cross section through the container 1 in a plane 15 running parallel to the base 5. Recurring features are provided with the same reference symbols in this and in the following figures. The vessel 2 has a cylindrical shape and the filter 12 divides the interior space 4 centrally, so that the first compartment 13 and the second compartment 14 each have the shape of a half cylinder. In FIG. 2, projections of the positions of accesses 7, 8 arranged above the cross section shown are also shown.
    Alternatively, in addition to a cylindrical design of the container 1, many different spatial shapes of the container 1 and in particular of the first compartment 13 and second compartment 14 are also possible.
    A second embodiment of a container is shown in FIG. Similar features have been given similar reference numbers in this and subsequent figures, i. Similar features have reference numbers increased by 100 or a multiple of 100. The container 101 again comprises a lid 103, a vessel 102 and a filter 112. In this embodiment, a first septum 109 and a second septum 110 are arranged below a first access 107 and a second access 108 and are attached to the lid 103. The first septum 109 and the second septum 110 are not firmly connected to one another in this exemplary embodiment. In contrast, the septa 109, 110 can be designed in such a way that they can be fastened individually to the cover 103 by snapping into place. A one-piece design of the first septum 109 and the second septum 110 is also possible.
    4 shows a third embodiment of a container 201. Here, a filter 212 is attached to a cover 203 below a first access 207 and has no contact region with a vessel 202. FIG. 5 shows a cross section through the vessel 202 and the filter 212 in a plane running parallel to a standing surface 205 of the container 201. In addition, projections of the positions of accesses 207, 208 arranged above the cross section shown are shown. The filter 212 has a square cross section, so that a first compartment 213 is cube-shaped. Other geometric shapes of the filter 212 are also possible, for example, it can be cylindrical, funnel-shaped or conical. The first compartment 213 is separated from a second compartment 214 by the filter 212.
    When the container 1 is manufactured, the filter 12 can first be attached to the cover 3 or to the vessel 2 under sterile conditions. The vessel 2 can then be closed by the cover 3 under likewise sterile conditions. A completely sterile pre-assembly of the container 1 is thus provided, so that the interior space 4 of the container 1 is sterile when the container 1 is delivered. The container 1 can also be sterilized, for example, immediately before use or after use, for example by means of ethylene oxide, gamma radiation or electron radiation. The container 1 can then be filled with a liquid or a liquid can be withdrawn from the container 1 through the ports, which, when using suitable septa 9, 10, does not allow germs capable of replication to penetrate into the container 1. Thus, in a typical use of the container 1, opening of the container 1 is never necessary.
    An exemplary filled container 1 according to the first embodiment is shown in Fig. 6, i. at a point in time after a suspension 16 has been filled into the second compartment 14 through the second access 8. The suspension 16 comprises a solvent and furthermore contains particles 17 with a diameter of 10 μm. The filter 12 is a membrane filter made of polypropylene and has pores with a diameter of 1 μm. The membrane filter is permeable to the solvent, which is why a common liquid level is established in the first compartment 13 and in the second compartment 14. Since the particles 17 are larger than the pores, the particles 17 do not pass through the filter 12 into the first compartment 13, but remain in the second compartment 14. In the first compartment 13, a filtrate 18 from the solvent and others collects Suspension 16 containing substances such as metabolic products that have a smaller diameter than the pores or are optionally dissolved in the filtrate 18. The filtrate 18 can then be removed from the first compartment 13 through the first access 7, for example in order to feed it to a high-performance liquid chromatograph.
    In Fig. 7, the first embodiment of the container 1 is shown again. A first syringe needle 19 and a second syringe needle 20 are also shown. The first syringe needle 19 is used to remove the filtrate 18 from the first compartment 13 and the second syringe needle 20 to pour a suspension 16 into the second compartment 14. The syringe needles 19, 20 pierce the respective septa 9, 10 in such a way that the interior 4 of the container 1 is furthermore separated from the surroundings 21 of the container 1 in a sterile manner.
    The filling of the suspension 16 into the second compartment 14 takes place under an increased pressure relative to the pressure which is present in a removed filtrate. A pressure difference between a suspension reservoir on the filling side and a filtrate reservoir on the withdrawal side is provided by a sample processing machine.
    In this example, the suspension 16 can be continuously filtered, i. At any point in time, the suspension 16 flows into the container 1 and the filtrate 18 flows out of the container 1. However, it is also possible, for example, for the suspension 16 to flow in and the filtrate 18 to flow out alternately. During these processes, a concentration of the particles 17 in the second compartment 14 increases steadily.
    In addition, steps can be carried out which provide for the container 1 to be rinsed with a further liquid in order to clean it from particles 17. For this purpose, it can also be provided that, compared to the filtration, the respective other access is used for an inflow or an outflow of a liquid.
    The accesses 7, 8 are typically round and typically have a diameter of about 5 mm. However, larger diameters of, for example, 10 mm or smaller diameters are also possible. Furthermore, the first access 7 can, for example, have a diameter that differs from a diameter of the second access 8. The accesses 7, 8 are typically arranged at a distance from one another so that the accesses 7, 8 are also easily accessible for typical syringes or other supply or removal devices at the same time. Depending on the application, a distance from the centers of the accesses 7, 8 of 10 mm or more can represent a sufficient spacing of the accesses 7, 8, for example.
    A top view of the container 1 of the first embodiment and a receptacle 22 of an autosampler is shown in FIG. The autosampler is a part of the sample processing machine which is set up for an automatic filling of the suspension 16 into the container 1 and for an automatic removal of the filtrate 18 from the container 1. The first access 7 and the second access 8 are in the cover 3 of the Container 1 arranged side by side. With the exception of the positioning aid 11, the cover 3 has a cylindrical outer boundary surface 23. 8 also shows, by way of example, a course of the filter 12 arranged in the vessel 2 below the cross section shown.
    So that the first access 7 and the second access 8 are arranged in a defined position relative to the receptacle 22, a recess 24 is provided in the receptacle 22, through which the positioning aid 11 when the container 1 is inserted into the receptacle 22 is performed in such a way that an orientation of the container 1 in the receptacle 22 is determined. The illustrated positioning aid 11 is designed in the form of an outwardly directed extension on the cover 3. In addition, a positioning aid 11 can be provided additionally or exclusively on the vessel 2, for example.
    It is also possible for a positioning aid 11 to be designed as a groove on the cover 3 or on the vessel 2 of the container 1. In this case, instead of a recess in the receptacle 22, an indentation can also be provided which is designed to engage in the groove on the cover 3 or on the vessel 2. In addition, it is possible for the positioning aid 11 to be designed as a pin on the vessel 2 and / or on the cover 3. It can also be provided that the positioning aid 11 is present as a flattened side of the vessel 2 and / or of the lid 3.
    Because the positioning aid 11 is provided so that the position of the accesses 7, 8 is fixed relative to a receptacle 22, a positioning aid 11 attached to the vessel 2 is particularly suitable for designs in which the lid 3 has a defined alignment with respect to the Has vessel 2, in particular the lid 3 should not be attached to the vessel 2 such that it can rotate in an uncontrolled manner.
    In Fig. 9 and Fig. 10, a fourth embodiment of a container 301 is shown. In this embodiment, a first access 307 lies in the center of the cover 303. A second access 308 is arranged concentrically around the first access 307 in the form of a circular ring. A filter 312 is arranged such that a first compartment 313 extends from the first access 307 to an underside 306 of a vessel 302. A second compartment 314 surrounds an upper area of the first compartment 313 in the form of a cylinder ring and is arranged below the second access 308. On an underside 325 of the cover 303, the first access 307 has a circular first septum 309 and the second access 308 has a circular second septum 310.
    In this embodiment, the container is constructed, for example, completely rotationally symmetrical. A positioning aid 11 is not shown in this example. However, it is possible that a rotational symmetry is deliberately broken by a positioning aid 11 on the vessel 302 or on the lid 303.
    11 shows a further exemplary embodiment of a container 401. The container 401 differs from the containers according to the previously described exemplary embodiments in that it comprises a filter 412 in the form of a hollow fiber membrane. The filter 412 is attached to a cover 403, which in turn is connected to a vessel 402. The hollow fiber membrane has a cavity that forms a first compartment 413. The first compartment 413 is connected to a first access 407 on the cover 403. A chamber outside the hollow fiber membrane forms a second compartment 414 and is connected to a second access 408 on the cover 403. The cover 403 also has a pressure compensation filter 426. This is designed in the form of a hydrophobic membrane firmly connected to the cover 403 with an average pore size of 0.15 μm and serves a sterile pressure equalization between an interior 404 of the container 401 and the environment 21. Alternatively, the filter 412 can also be designed as a hollow fiber bundle so that a large filter surface is achieved. The hollow fiber bundle consists, for example, of a multiplicity of hollow fibers that are intertwined. Such a hollow fiber bundle can be present in the interior 404 in various forms. For example, a spiral-shaped hollow fiber bundle or a ball-shaped hollow fiber bundle is possible.
    A side view of a further exemplary embodiment of a container 501 with a lid 503 and a vessel 502 is shown in FIG. The lid 503 is made of a thermoplastic material and is connected to the vessel 502. The vessel 502 is designed in the form of a bag made of polyethylene with a wall thickness of 0.1 mm. In this exemplary embodiment, the vessel 502 does not have a standing surface 5. As a result, the vessel 502 has to be clamped into a holder for a horizontal position of the lid 503, for example on the lid 503. As indicated in FIG. 12, the container 501 has a first access 507, a second access 508, a pressure compensation filter 527 and a filter 512. The filter 512 is connected to the lid 503 and divides an interior of the container 501 into a first compartment 513 and a second compartment 514, the first access 507 being connected to the first compartment 513 and the second access 508 being connected to the second compartment 514. In this example, the filter 512 is designed as a flexible membrane. Because both the vessel 502 and the filter 512 are not rigid, but rather deformable, such vessels can be stacked and stored in a space-saving manner.
    A cross section of a further exemplary embodiment of a container 601 is shown in FIG. Here, a cover 603 comprises a first access 607 and a second access 608, each of which has septa 609, 610, and a pressure compensation filter 626. A further pressure compensation filter 627 is provided on a vessel 602. The vessel 602 has a cylindrical shape and is rounded on its underside 606, which makes it difficult to place the container 601 on a flat surface. A filter 612, which separates a first compartment 613 and a second compartment 614 from one another, is designed as a concentrically arranged ceramic tube with an average pore size of 5 μm and is at a lower end in contact with the bottom 606 of the vessel 602 possible that the filter (for example the ceramic tube) is made shorter and closed at a lower end and is arranged in such a way that it does not extend as far as the underside 606 of the vessel 602.
    14 shows a plan view of a further exemplary embodiment of a cover 703. This has an essentially round outline and comprises a positioning aid 711 in the form of a flattened side. A first access 707 is set up for filling with a suspension, has a round outline and is arranged in the center of the cover 703. Around the first access 707, two second accesses, two of which are provided with the reference numerals 708 and 708 ', are arranged with round outlines and of smaller diameters. The second accesses 708, 708 'each have septa, two of which are provided with the reference numerals 710 and 710' by way of example, and are set up to remove a filtrate. However, it is also possible that a second access for removing the filtrate is arranged in the center of the lid and that several first accesses for filling the container with a suspension are arranged around this second access.
    15 shows a further exemplary embodiment of a container 801. The container 801 comprises a lid 803 with a first access 807 and a second access 808, which each have septa 809, 810. In addition, the container 801 comprises a vessel 802, in which a filter 812 designed as a membrane is enclosed at an angle. The filter 812 separates an interior 804 of the vessel 802 into a first compartment 813, which is connected to the first access 807, and a second compartment 814, which is connected to the second access 808 via a liquid-tight tube 828. The filter 812 and the tube 828 are designed in such a way that an edge of a recess in the filter 812 encloses an outside of the tube 828.
    In Fig. 16, the container 801 of Fig. 15 is shown again, with the difference that the filter 812 is designed in this variant in the shape of a cone. In this embodiment, the filter 812 has a lowest point 829. If the filter 812 is formed from a flexible material, then the lowest point 829 is not rigidly bound to a location in the interior space 804. In this embodiment, for example, a liquid can be filled into the first compartment through the first access, whereupon the liquid collects in an area near the lowest point 829 and is also mainly filtered in this area by the filter 812. The liquid can then be withdrawn in filtered form through the tube 828 via the second access 808.
    Features of the various embodiments that are only disclosed in the exemplary embodiments can be combined with one another and claimed individually.
    权利要求:
    Claims (15)
    [1]
    1. A container (1) for filtering a suspension (16) comprising a lid (3) and a vessel (2), the container (1) having a filter (12) which has an interior (4) of the container (1) divided into a first compartment (13) and a second compartment (14), the cover (3) having a first access (7) and a second access (8) and the first access (7) with the first compartment (13) and the second access (8) is connected to the second compartment (14).
    [2]
    2. Container (1) according to claim 1, characterized in that the filter (12) is arranged such that the first compartment (13) and the second compartment (14) each from a part of the vessel (2), from a part of the lid (3) and the filter (12).
    [3]
    3. Container (1) according to claim 1, characterized in that the filter (12) is arranged such that the first compartment (13) or the second compartment (14) exclusively from a part of the lid (3) and from the filter (12) is limited.
    [4]
    4. Container (1) according to one of claims 1 to 3, characterized in that the filter (12) is a membrane.
    [5]
    5. Container (1) according to one of claims 1 or 4, characterized in that the filter (12) has pores with a diameter of at least 0.02 µm and / or at most 10 µm.
    [6]
    6. Container (1) according to one of claims 1 to 5, characterized in that the interior (4) has a volume of at least 0.1 ml and / or at most 50 ml.
    [7]
    7. Container (1) according to one of claims 1 to 6, characterized in that the vessel (2) and / or the lid (3) comprises a thermoplastic material.
    [8]
    8. Container (1) according to one of claims 1 to 7, characterized in that the first access (7) and / or the second access (8) has a septum (9, 10).
    [9]
    9. Container (1) according to one of claims 1 to 8, characterized in that the vessel (2) has a cylindrical shape.
    [10]
    10. Container (1) according to one of claims 1 to 9, characterized by a positioning aid (11), wherein the positioning aid (11) is designed to guide the container (1) in a fixed orientation into a container receptacle (22).
    [11]
    11. Container (1) according to one of claims 1 to 10, characterized by at least one pressure compensation filter (26, 27) which provides pressure compensation between the first compartment (13) and / or the second compartment (14) and an environment (21) of the container (1) allows.
    [12]
    12. Container (1) according to one of claims 1 to 11, characterized in that the first access (7) and / or the second access (8) is suitable for treating a suspension (16) located in the interior (4) with ultrasound .
    [13]
    13. A method for filtering a suspension (16) with the following steps:- Provision of a container (1) comprising a lid (3) and a vessel (2), the container (1) having a filter (12) which divides an interior space (4) of the container (1) into a first compartment (13 ) and a second compartment (14), the cover (3) having a first access (7) and a second access (8) and the first access (7) with the first compartment (13) and the second access (8 ) is connected to the second compartment (14),- Filling a liquid to be filtered through the first access (7) or through the second access (8) into the interior (4),- Removal of a filtered liquid through the second access (8) or first access (7) from the interior.
    [14]
    14. The method according to claim 13, characterized in that the filling and the removal take place simultaneously.
    [15]
    15. The method according to any one of claims 13 or 14, characterized by the additional step:- Applying a pressure difference between the first access (7) and the second access (8).
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    同族专利:
    公开号 | 公开日
    DE102015216241A1|2017-03-02|
    WO2017032845A1|2017-03-02|
    US20180243695A1|2018-08-30|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    IL60645A|1980-07-21|1984-02-29|Cais Michael|Method and device for mass transfer and separation through selective barriers|
    DE3422435A1|1984-06-16|1986-01-16|B. Braun Melsungen Ag, 3508 Melsungen|METHOD AND DEVICE FOR SELECTIVELY SEPARATING PATHOLOGICAL AND / OR TOXIC SPECIES FROM BLOOD OR BLOOD PLASMA USING FILTER CANDLES|
    US5316665A|1992-10-16|1994-05-31|Hart Robert M|Pre-filter oil inspection screen|
    US5888831A|1997-03-05|1999-03-30|Gautsch; James W.|Liquid-sample-separation laboratory device and method particularly permitting ready extraction by syringe of the separated liquid sample|
    JPH10314552A|1997-05-15|1998-12-02|Mitsubishi Rayon Co Ltd|Hollow fiber membrane cartridge for capturing protozoan and method for capturing and collecting protozoan|
    US7288195B2|1999-05-28|2007-10-30|Bio/Data Corporation|Method and apparatus for directly sampling a fluid for microfiltration|
    DE19952757A1|1999-11-02|2001-05-03|Brita Gmbh|Filter device for liquids|
    JP4299537B2|2000-11-09|2009-07-22|三菱レイヨン株式会社|Centrifuge cartridge, microorganism capture and recovery cartridge, and microorganism capture concentration and recovery method|
    DE10259661A1|2002-10-16|2004-04-29|Beko Technologies Gmbh|Hollow fiber module|
    DE10309428B4|2003-03-05|2005-09-15|Ultrafilter International Ag|filter|
    CH705468A1|2011-09-06|2013-03-15|Tecan Trading Ag|Chamber system and sample containers with inlet.|DE102018102383B4|2018-02-02|2019-08-29|Scienova Gmbh|System for carrying out biological or chemical processes|
    IT201800004625A1|2018-04-17|2019-10-17|DEVICE FOR THE SEPARATION OF A BIOLOGICAL SAMPLE|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    DE102015216241.9A|DE102015216241A1|2015-08-25|2015-08-25|Container and method for filtering a suspension|
    PCT/EP2016/070109|WO2017032845A1|2015-08-25|2016-08-25|Analysis container and method for the filtration of a suspension with the use thereof|
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