endophytic compositions and methods for improving plant traits

专利摘要:
This invention relates to methods and materials for modulating the characteristics of a plant, in which said plant is heterologously arranged in an endophyte organism or in a plurality of endophyte organisms, or derived from a plant reproductive element, heterologously, in an endophyte organism or a plurality of endophyte organisms.
公开号:BR112019018232A2
申请号:R112019018232-2
申请日:2017-12-01
公开日:2020-07-28
发明作者:Raymond Riley
申请人:Indigo Ag, Inc.；
IPC主号:

专利说明:

[0001] [0001] This request claims priority to Provisional Application nº62 / 551,724, filed on August 29, 2017; Provisional Application No. 62 / 467,734, filed on March 6, 2017; Provisional Application No. 62 / 467,737, filed on March 6, 2017; Provisional Application No. 62 / 466,250, filed on March 2, 2017; Provisional Application No. 62/465,820, filed on March 2, 2017; Provisional Application No. 62/465,833, filed on March 2, 2017; and Provisional Order No. 62 / 465,818, filed on March 1, 2017, whose disclosures are incorporated by reference in their entirety. SEQUENCE LISTING
[0002] [0002] The immediate order contains a Sequence Listing with 51 sequences that was submitted via EFS-Web and is incorporated by reference through this document in its entirety. Said ASCII copy, created on November 29, 2017, is called 39060 10100 Sequence Listing.txt and has
[0003] [0003] The present invention relates to compositions and methods to improve the traits of plants, particularly plants important for human or animal consumption, for example rice (Oryza sativa and related varieties), soybeans (Glycine max and related varieties), wheat (Triticum aestivum and related varieties) and corn (Zea mays and related varieties). For example, this invention describes microbes that are capable of living within or heterologously disposed of a plant, and that can be used to impart enhanced traits to the plants with which they are or have been heterologously disposed. The disclosed invention also describes methods to improve the characteristics of plant elements by introducing microbes to the original plants. In addition, this invention also provides methods of treating plant elements with microbes that are capable of living within a plant, particularly rice, soybeans, wheat and corn, to impart better yield and other agronomic characteristics to that plant. BACKGROUND OF THE INVENTION
[0004] [0004] According to the United Nations Food and Agriculture Organization, the world population will exceed 9.6 billion people by 2050, which will require significant improvements in agriculture to meet the growing demand for food. There is a need for improved agricultural plants that allow for almost doubled demand for food production with fewer resources and more environmentally sustainable inputs for plants with improved responses to various biotic and abiotic stresses.
[0005] [0005] Currently, crop performance is optimized mainly through technologies aimed at the interaction between the genotype of the crops (for example, plant reproduction, genetically modified (GM) crops) and their surrounding environment (for example, fertilizers, herbicides synthetics, pesticides). Although these paradigms have helped to double global food production over the past fifty years, production growth rates have stagnated in many important crops and changes in climate have been linked to production instability as well as changing pressures from pests and diseases , leading to an urgent need for new solutions for crop improvement. In addition to their long regulatory and development timelines, public fears of GM crops and synthetic chemicals have challenged their use in many crops and countries, resulting in the lack of acceptance of many GM traits and the exclusion of GM crops and many crops. synthetic technologies of some global markets. Thus, there is a significant need for innovative, effective, environmentally sustainable and publicly acceptable approaches to improve yield and other agronomically important characteristics of plants.
[0006] [0006] In this document, methods and compositions are provided to improve the agronomically important characteristics of plants, associating these plants with disclosed endophytes. SUMMARY OF THE INVENTION
[0007] [0007] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant, comprising disposing, in a heterologous manner, of an endophyte organism to an element of the soybean plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33.
[0008] [0008] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soy plant comprising heterologously arranging an endophyte organism for an element of the soy plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466.
[0009] [0009] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soy plant comprising heterologously disposing an endophyte organism for an element of the soy plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a modified endophyte organism from genus Periconia derived from the endophyte organism, as deposited under the NRRL Culture Deposit No. 67466.
[0010] [0010] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33.
[0011] [0011] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, dry shoot biomass and yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466.
[0012] [0012] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a modified endophyte of the genus Periconia derived from the endophyte, as deposited under NRRL Culture Deposit No. 67466.
[0013] [0013] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleotic sequence at least 97% identical to a polynucleot sequence selected tip from the group consisting of SEQ ID NO: 32 and 33.
[0014] [0014] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the corn plant in an effective amount to increase yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466.
[0015] [0015] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a modified endophyte of the genus Periconia derived from the endophyte, as deposited under NRRL Culture Deposit No. 67466.
[0016] [0016] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the soybean plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 34-38.
[0017] [0017] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the soybean plant in an amount effective to increase a trace of agronomic importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Curvularia, as deposited under the NRRL Culture Deposit No.
[0018] [0018] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the soybean plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is an endophyte organism modified genus Curvularia derived from the endophyte organism, as deposited under the NRRL Culture Deposit No.
[0019] [0019] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 34-38.
[0020] [0020] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Curvularia, as deposited under o NRRL Culture Deposit No. 67467.
[0021] [0021] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67467.
[0022] [0022] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97% identical to a polynucleot sequence tip selected from the group consisting of SEQ ID NOs: 34-38.
[0023] [0023] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the corn plant in an amount effective to increase yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endáophyte is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467.
[0024] [0024] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a modified endophyte of the genus Curvularia derived from the endophyte, as deposited under NRRL Culture Deposit No. 67467.
[0025] [0025] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Aspergillus and comprises at least one polynucleotic sequence at least 97% identical to SEQ ID NO: 39.
[0026] [0026] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, root length, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the organism endophyte is a member of the genus Coniochaeta and comprises at least one polynucleot tip sequence at least 97% identical to a polynucleot tip sequence selected from the group consisting of SEQ ID NOs: 40-43.
[0027] [0027] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the rice plant in an amount effective to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 40-43.
[0028] [0028] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate and dry biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Pestalotiopsis e comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 44-48.
[0029] [0029] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the rice plant in an amount effective to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus
[0030] [0030] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the corn plant in an amount effective to increase biomass of dry root in the plant derived from the element of the treated plant relative to a plant derived from an element of the reference plant, in which the endophyte organism is a member of the genus Enterobacter, as deposited under NRRL Culture Deposit No. B67465.
[0031] [0031] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the rice plant in an amount effective to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a modified endobophyte organism of the genus Enterobacter, derived from the endophyte organism, as deposited under the Deposit of NRRL Culture No. B67465.
[0032] [0032] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant, comprising disposing, in a heterologous manner, of an endophyte organism for an element of the soybean plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33 and in which the plant element is a seed.
[0033] [0033] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the soybean plant in an amount effective to increase a trace of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466, in which the plant element is a seed.
[0034] [0034] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the soybean plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a modified endophyte organism from genus Periconia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67466, in which the plant element is a seed.
[0035] [0035] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the plant element is a seed.
[0036] [0036] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, dry shoot biomass and yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Periconia, as deposited under the NRRL Culture Deposit No. 67466, in which the plant element is a seed.
[0037] [0037] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a modified endophyte of the genus Periconia derived from the endophyte, as deposited under NRRL Culture Deposit No. 67466, in which the plant element is a seed.
[0038] [0038] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97% identical to a polynucleot sequence tip selected from the group consisting of SEQ ID NO: 32 and 33, in which the plant element is a seed.
[0039] [0039] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the corn plant in an amount effective to increase yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466, where the plant element it is a seed.
[0040] [0040] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an effective amount to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a modified endophyte of the genus Periconia derived from the endophyte, as deposited under NRRL Culture Deposit No. 67466, where the plant element is a seed.
[0041] [0041] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the soybean plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the plant element is a seed.
[0042] [0042] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soy plant comprising heterologously arranging an endophyte organism for an element of the soy plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Curvularia , as deposited under NRRL Culture Deposit No. 67467, in which the plant element is a seed.
[0043] [0043] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the soybean plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is an endophyte organism modified genus Curvularia derived from the endophyte organism, as deposited under the NRRL Culture Deposit No.
[0044] [0044] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the plant element is a seed.
[0045] [0045] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467, in which the plant element is a seed.
[0046] [0046] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67467, in which the plant element is a seed.
[0047] [0047] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleot sequence tip selected from the group consisting of SEQ ID NOs: 34-38, in which the plant element is a seed.
[0048] [0048] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the corn plant in an effective amount to increase yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endáophyte is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467, where the plant element it is a seed.
[0049] [0049] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a modified endophyte of the genus Curvularia derived from the endophyte, as deposited under NRRL Culture Deposit No. 67467, where the plant element is a seed.
[0050] [0050] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Aspergillus and comprises at least one polynucleotic sequence at least 97% identical to SEQ ID NO: 39, in which the plant element is a seed.
[0051] [0051] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, root length, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the organism endophyte is a member of the genus Coniochaeta and comprises at least one polynucleot tip sequence at least 97% identical to a polynucleot tip sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the plant element is a seed.
[0052] [0052] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the rice plant in an amount effective to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97% identical to a polynucleot hint selected from the group consisting of SEQ ID NOs: 40-43, in which the plant element is a seed.
[0053] [0053] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate and dry biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Pestalotiopsis e comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 44-48, in which the plant element is a seed.
[0054] [0054] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the rice plant in an amount effective to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97% identical to a polynucleot hint selected from the group consisting of SEQ ID NOs: 49-51, in which the plant element is a seed.
[0055] [0055] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the corn plant in an amount effective to increase biomass of dry root in the plant derived from the element of the treated plant relative to a plant derived from an element of the reference plant, where the endophyte organism is a member of the genus Enterobacter, as deposited under NRRL Culture Deposit No. B67465, where the plant element is a seed.
[0056] [0056] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the rice plant in an amount effective to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a modified endophyte organism of the genus Enterobacter, derived from the endophyte organism, as deposited under the Deposit of NRRL Culture No. B67465, where the plant element is a seed.
[0057] [0057] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant, comprising disposing, in a heterologous manner, of an endophyte organism for an element of the soybean plant in an effective amount to increase a trait of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the seed is modified.
[0058] [0058] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the soybean plant in an amount effective to increase a trace of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466, in which the seed is modified.
[0059] [0059] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the soybean plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a modified endophyte organism from genus Periconia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67466, in which the seed is modified.
[0060] [0060] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the plant element is a seed, in which the seed is modified.
[0061] [0061] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, dry shoot biomass and yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466, in which the seed is modified.
[0062] [0062] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a modified endophyte of the genus Periconia derived from the endophyte, as deposited under NRRL Culture Deposit No. 67466, in which the plant element is a seed, in which the seed is modified.
[0063] [0063] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97% identical to a polynucleot sequence tip selected from the group consisting of SEQ ID NO: 32 and 33, in which the plant element is a seed, in which the seed is modified.
[0064] [0064] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising arranging, in a heterologous manner, an endophytic organism for an element of the corn plant in an effective amount to increase yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466, in which the seed is modified .
[0065] [0065] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a modified endophyte of the genus Periconia derived from the endophyte, as deposited under NRRL Culture Deposit No. 67466, where the plant element is a seed, in which the seed is modified.
[0066] [0066] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the soybean plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the plant element is a seed, in which the seed is modified .
[0067] [0067] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soy plant comprising heterologously arranging an endophyte organism for an element of the soy plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Curvularia , as deposited under NRRL Culture Deposit No. 67467, in which the plant element is a seed, in which the seed is modified.
[0068] [0068] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soy plant comprising heterologously disposing an endophyte organism for an element of the soy plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is an endophyte organism modified from the genus Curvularia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67467, in which the plant element is a seed, in which the seed is modified.
[0069] [0069] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the plant element is a seed, in which the seed is modified.
[0070] [0070] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467, in which the plant element is a seed, in which the seed is modified.
[0071] [0071] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophytic organism, as deposited under NRRL Culture Deposit No. 67467, in which the plant element is a seed, in which the seed is modified.
[0072] [0072] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97% identical to a polynucleot sequence is selected from the group consisting of SEQ ID NOs: 34-38, in which the plant element is a seed, in which the seed is modified.
[0073] [0073] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising heterologously arranging an endophyte organism for an element of the corn plant in an effective amount to increase yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endáophyte is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467, in which the seed is modified .
[0074] [0074] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the corn plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a modified endophyte of the genus Curvularia derived from the endophyte, as deposited under NRRL Culture Deposit No. 67467, where the plant element is a seed, in which the seed is modified.
[0075] [0075] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase the plant yield derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Aspergillus and comprises at least one polynucleotic sequence at least 97% identical to SEQ ID NO: 39, where the plant element is a seed, where the seed is modified.
[0076] [0076] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, root length, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the organism endophyte is a member of the genus Coniochaeta and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the plant element is a seed, in which the seed is modified.
[0077] [0077] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the rice plant in an amount effective to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97% identical to a polynucleot hint selected from the group consisting of SEQ ID NOs: 40-43, in which the plant element is a seed, in which the seed is modified.
[0078] [0078] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate and dry biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 44-48, in which the plant element is a seed, in which the seed is modified.
[0079] [0079] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the rice plant in an amount effective to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Enterobacter and comprises at least one polynucleotic sequence at least 97% identical to a polynucleot hint selected from the group consisting of SEQ ID NOs: 49-51, in which the plant element is a seed, in which the seed is modified.
[0080] [0080] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the corn plant in an amount effective to increase biomass of dry root in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a member of the genus Enterobacter, as deposited under NRRL Culture Deposit No. B67465, where the plant element is a seed, in which the seed is modified.
[0081] [0081] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the rice plant in an amount effective to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a modified endophyte organism of the genus Enterobacter, derived from the endophyte organism, as deposited under the Deposit of NRRL Culture No. B67465, where the plant element is a seed, where the seed is modified.
[0082] [0082] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant, comprising disposing, in a heterologous manner, of an endophyte organism for an element of the soybean plant in an effective amount to increase a trait of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the endophyte arranged, heterologously, to the plant element in a formulation comprising one or more of the following: a stabilizer, a preservative, a carrier, a surfactant, a fungicide, a nematicide, a bactericide, an in seticide or a herbicide or any combination thereof.
[0083] [0083] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the soybean plant in an amount effective to increase a trace of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466, in which the endophyte is heterologously disposed of the plant element in a formulation comprising one or more of the following: a stabilizer, a preservative, a carrier, a surfactant, a fungicide, a nematicide, a bactericide, an insecticide or an herbicide or any combination thereof.
[0084] [0084] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the soybean plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a modified endophyte organism from genus Periconia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67466, in which the endophyte disposed, in a heterologous manner, to the plant element in a formulation comprising one or more of the following: a stabilizer, a preservative , a carrier, a surfactant, a fungicide, a nematicide, a bactericide, an insecticide or an herbicide or any combination thereof.
[0085] [0085] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant, comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, dry shoot biomass and yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the endophyte disposed, heterologously, to the element of plant in a formulation comprising one or more of the following: a stabilizer, a preservative, a carrier, a surfactant, a fungicide, a nematicide, a bactericide, an insecticide or a herbicide or any combination thereof.
[0086] [0086] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, dry shoot biomass and yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466, in which the endophyte is heterologously disposed of the plant element in a formulation comprising one or more of the following: a stabilizer, a preservative, a carrier, a surfactant, fungicide, nematicide, bactericide, insecticide or herbicide or any combination thereof.
[0087] [0087] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is an organism end ophite modified from the genus Periconia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67466, in which the endophyte disposed, heterologously, to the plant element in a formulation comprising one or more of the following: a stabilizer , a preservative, a carrier, a surfactant, a fungicide, a nematicide, a bactericide, an insecticide or an herbicide or any other ombination of these.
[0088] [0088] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant, comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an effective amount to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleotic sequence at least 97% identical to a sequence polynucleot tip selected from the group consisting of SEQ ID NO: 32 and 33, in which the endophyte is heterologously disposed of the plant element in a formulation comprising one or more of the following: a stabilizer, a preservative, a carrier, a surfactant, a fungicide, a nematicide, a bactericide, an insecticide or an herbicide or any combination thereof.
[0089] [0089] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the corn plant in an effective amount to increase yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466, in which the disposed endophyte, in heterologous form, to the plant element in a formulation comprising one or more of the following: a stabilizer, a preservative, a carrier, a surfactant, a fungicide, a nematicide, a bactericide, an insecticide or a herbicide or any combination thereof .
[0090] [0090] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67466 , in which the endophyte is heterologously disposed of the plant element in a formulation comprising one or more of the following: a stabilizer, a preservative, a carrier, a surfactant, a fungicide, a nematicide, a bactericide, a insecticide or a herbicide or any combination thereof.
[0091] [0091] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant, comprising disposing, in a heterologous manner, of an endophyte organism for an element of the soybean plant in an effective amount to increase a trait of agronomic importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the endophyte arranged, heterologously, to the plant element in a formulation comprising one or more of the following: a stabilizer, a preservative, a carrier, a surfactant, a fungicide, a nematicide, a bacteri an insecticide or herbicide or any combination thereof.
[0092] [0092] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the soybean plant in an amount effective to increase a trace of agronomic importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Curvularia , as deposited under NRRL Culture Deposit No. 67467, in which the endophyte is heterologously disposed of the plant element in a formulation comprising one or more of the following: a stabilizer, a preservative, a carrier, a surfactant , a fungicide, a nematicide, a bactericide, an insecticide or a herbicide or any combination thereof.
[0093] [0093] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the soybean plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is an endophyte organism modified from the genus Curvularia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67467, in which the endophyte disposed, heterologously, to the plant element in a formulation comprising one or more of the following: a stabilizer, a preservative, a carrier, a surfactant, a fungicide, a nematicide, a bactericide, an insecticide or an herbicide or any combination of these.
[0094] [0094] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant, comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the endophyte arranged heterologously to the plant element in a formulation comprising one or more of the following: a stabilizer, a preservative, a carrier, a surfactant, a fungicide, a nematicide, a bactericide, an insecticide ticide or a herbicide or any combination thereof.
[0095] [0095] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467, in which the endophyte is heterologously disposed of the plant element in a formulation comprising one or more of the following: a stabilizer, a preservative, a carrier, a surfactant, a fungicide, a nematicide, bactericide, insecticide or herbicide or any combination thereof.
[0096] [0096] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67467, in which the endophyte disposed, heterologously, to the plant element in a formulation comprising one or more of the following: a stabilizer, a preservative, a carrier, surfactant, fungicide, nematicide, bactericide, insecticide or herbicide or any combination thereof.
[0097] [0097] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant, comprising disposing, in a heterologous manner, of an endophyte organism for an element of the corn plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a sequence polynucleot tip selected from the group consisting of SEQ ID NOs: 34-38, in which the endophyte is heterologously disposed of the plant element in a formulation comprising one or more of the following: a stabilizer, a preservative, a carrier, a surfactant, a fungicide, a nematicide, a bactericide, an insecticide or an herbicide or any combination thereof.
[0098] [0098] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the corn plant in an effective amount to increase yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467, in which the disposed endophyte, in heterologous form, to the plant element in a formulation comprising one or more of the following: a stabilizer, a preservative, a carrier, a surfactant, a fungicide, a nematicide, a bactericide, an insecticide or a herbicide or any combination thereof .
[0099] [0099] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67467 , in which the endophyte is heterologously disposed of the plant element in a formulation comprising one or more of the following: a stabilizer, a preservative, a carrier, a surfactant, a fungicide, a nematicide, a bactericide, a insecticide or a herbicide or any combination thereof.
[00100] [00100] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant, comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleotic sequence at least 97% identical to SEQ ID NO: 39, in which the endophyte disposed, heterologously, to the plant element in a formulation comprising one or more of the following: a stabilizer, a preservative, a carrier, a surfactant, a fungicide, a nematicide, a bactericide, an insecticide or a herbicide or any combination thereof.
[00101] [00101] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant, comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, root length, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the endophyte is heterologously disposed , to the plant element in a formulation comprising one or more of the following: a stabilizer, a preservative, a carrier, a surfactant, a fu ngicide, nematicide, bactericide, insecticide or herbicide or any combination thereof.
[00102] [00102] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant, comprising disposing, in a heterologous manner, of an endophyte organism for an element of the rice plant in an effective amount to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the endophyte is heterologously disposed of the plant element in a formulation comprising one or more of the following: a stabilizer , a preservative, a carrier, a surfactant, a fungicide, a nematicide, a bactericide, an insecticide or an herbicide or any combination thereof.
[00103] [00103] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant, comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate and dry biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 44-48, in which the endophyte arranged, heterologously, to the plant element in a formulation comprising one or more of the following: a stabilizer, a preservative, a carrier, a surfactant, a fungicide, a nematicide, a bactericide, an insecticide or a herbicide or any combination thereof.
[00104] [00104] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant, comprising disposing, in a heterologous manner, of an endophyte organism for an element of the rice plant in an effective amount to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the endophyte is heterologously disposed of the plant element in a formulation comprising one or more of the following: a stabilizer, a preservative, a carrier, a surfactant, a fungicide, a nematicide, a bactericide, an insecticide or an herbicide or any combination thereof.
[00105] [00105] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising arranging, in a heterologous manner, an endophyte organism for an element of the corn plant in an amount effective to increase biomass of dry root in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a member of the genus Enterobacter, as deposited under NRRL Culture Deposit No. B67465, where the endophyte organism is heterologously disposed to the plant element in a formulation that further comprises one or more of the following: a stabilizer, a preservative, a carrier, a surfactant, fungicide, a nematicide, a bactericide, an insecticide or herbicide, or any combination of these.
[00106] [00106] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophytic organism for an element of the rice plant in an effective amount to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a modified endophyte organism of the genus Enterobacter, derived from the endophyte organism, as deposited under the Deposit of NRRL Culture No. B67465, in which the endophyte organism is arranged heterologously to the plant element in a formulation that further comprises one or more of the following: a stabilizer, a preservative, a carrier, a surfactant, fungicide, a nematicide, a bactericide, an insecticide or herbicide, or any combination thereof.
[00107] [00107] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant, comprising disposing, in a heterologous manner, of an endophyte organism for an element of the soybean plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the percentage of identity is at least 98%.
[00108] [00108] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophytic organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, and in which the percentage of identity is at least 98%.
[00109] [00109] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus
[00110] [00110] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the soybean plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, and in which the percentage of identity is at least 98%.
[00111] [00111] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 34-38, where the percentage of identity is at least 98%.
[00112] [00112] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Aspergillus and comprises at least one polynucleotic sequence at least 97% identical to SEQ ID NO: 39, in which the identity percentage is at least 98%.
[00113] [00113] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, root length, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the organism endophyte is a member of the genus Coniochaeta and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 40-43, where the identity percentage is at least 98%.
[00114] [00114] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the rice plant in an effective amount to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97% identical to a polynucleot hint selected from the group consisting of SEQ ID NOs: 40-43, where the percentage of identity is at least 98%.
[00115] [00115] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate and dry biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 44-48,
[00116] [00116] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the rice plant in an amount effective to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Enterobacter and comprises at least one polynucleotic sequence at least 97% identical to a polynucleot hint selected from the group consisting of SEQ ID NOs: 49-51, where the percentage of identity is at least 98%.
[00117] [00117] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant, comprising disposing, in a heterologous manner, of an endophyte organism for an element of the soybean plant in an effective amount to increase a trait of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, where the percentage of identity is at least 99%.
[00118] [00118] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, where the percentage of identity is at least 99%.
[00119] [00119] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an effective amount to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97% identical to a polynucleot sequence selected tip from the group consisting of SEQ ID NO: 32 and 33, and in which the identity percentage is at least 99%.
[00120] [00120] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the soybean plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is at least 99%.
[00121] [00121] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97% identical to a polynucleot sequence is selected from the group consisting of SEQ ID NOs: 34-38, where the percentage of identity is at least 99%.
[00122] [00122] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase the plant yield derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Aspergillus and comprises at least one polynucleotic sequence at least 97% identical to SEQ ID NO: 39, in which the identity percentage is at least 99%.
[00123] [00123] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, root length, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the organism endophyte is a member of the genus Coniochaeta and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 40-43, where the identity percentage is at least 99%.
[00124] [00124] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the rice plant in an effective amount to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Coniochaeta and comprises at least one polynucleot hint at least 97% identical to a polynucleot hint selected from the group consisting of SEQ ID NOs: 40-43, where the percentage of identity is at least 99%.
[00125] [00125] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate and dry biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 44-48, where the percentage of identity is at least 99%.
[00126] [00126] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the rice plant in an amount effective to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Enterobacter and comprises at least one polynucleotic sequence at least 97% identical to a polynucleot hint selected from the group consisting of SEQ ID NOs: 49-51, where the percentage of identity is at least 99%.
[00127] [00127] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant, comprising disposing, in a heterologous manner, of an endophyte organism for an element of the soybean plant in an effective amount to increase a trait of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, where the percentage of identity is 100%.
[00128] [00128] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate,
[00129] [00129] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97% identical to a polynucleot sequence selected tip from the group consisting of SEQ ID NO: 32 and 33, where the identity percentage is 100%.
[00130] [00130] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the soybean plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, where the percentage of identity is 100%.
[00131] [00131] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 34-38, where the percentage of identity is 100%.
[00132] [00132] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Aspergillus and comprises at least one polynucleotic sequence at least 97% identical to SEQ ID NO: 39, where the identity percentage is 100%.
[00133] [00133] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, root length, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the organism endophyte is a member of the genus Coniochaeta and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 40-43, where the percentage of identity is 100% .
[00134] [00134] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the rice plant in an amount effective to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97% identical to a polynucleot hint selected from the group consisting of SEQ ID NOs: 40-43, where the percentage of identity is 100%.
[00135] [00135] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate and dry biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 44-48, where the percentage of identity is 100%.
[00136] [00136] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the rice plant in an amount effective to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Enterobacter and comprises at least one polynucleotic sequence at least 97% identical to a polynucleot hint selected from the group consisting of SEQ ID NOs: 49-51, where the percentage of identity is 100%.
[00137] [00137] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soy plant, comprising disposing, in a heterologous manner, of an endophyte organism for an element of the soy plant in an effective amount to increase a trait of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the percentage of identity is determined along an alignment region. at least 100 nucleotides.
[00138] [00138] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the percentage of identity is determined over a region of alignment of at least 100 nucleotides.
[00139] [00139] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97% identical to a polynucleot sequence tip selected from the group consisting of SEQ ID NO: 32 and 33, and in which the percentage of identity is determined over an alignment region of at least 100 nucleotides.
[00140] [00140] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the soybean plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined along an alignment region. at least 100 nucleotides.
[00141] [00141] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over an alignment region of at least 100 nucleotides.
[00142] [00142] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Aspergillus and comprises at least one polynucleotic sequence at least 97% identical to SEQ ID NO: 39, in which the percentage of identity is determined over an alignment region of at least 100 nucleotides.
[00143] [00143] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, root length, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the organism endophyte is a member of the genus Coniochaeta and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 4043, in which the percentage of identity is determined over a alignment region of at least 100 nucleotides.
[00144] [00144] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the rice plant in an amount effective to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over an alignment region of at least 100 nucleotides.
[00145] [00145] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate and dry biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Pestalotiopsis e comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over an alignment region of at least 100 nucleotides.
[00146] [00146] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophytic organism for an element of the rice plant in an effective amount to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Enterobacter and comprises at least one polynucleotic sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the percentage of identity is determined over an alignment region of at least 100 nucleotides.
[00147] [00147] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant, comprising disposing, in a heterologous manner, of an endophytic organism for an element of the soybean plant in an effective amount to increase a trait of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the percentage of identity is determined over an alignment region of at least 200 nucleotides.
[00148] [00148] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the percentage of identity is determined over a hair alignment region minus 200 nucleotides.
[00149] [00149] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endáophyte organism is a member of the genus Periconia and comprises at least one polynucleotic sequence at least
[00150] [00150] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soy plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the soy plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus It curves and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over a hair alignment region minus 200 nucleotides.
[00151] [00151] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over an alignment region of at least 200 nucleot deos.
[00152] [00152] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Aspergillus and comprises at least one polynucleotic sequence at least 97% identical to SEQ ID NO: 39, in which the percentage of identity is determined over an alignment region of at least 200 nucleotides.
[00153] [00153] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, root length, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the organism endophyte is a member of the genus Coniochaeta and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 4043, in which the percentage of identity is determined over a alignment region of at least 200 nucleotides.
[00154] [00154] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the rice plant in an amount effective to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over an alignment region of at least 200 nucleotides.
[00155] [00155] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate and dry biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over an alignment region of at least 200 nucleotides.
[00156] [00156] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the rice plant in an amount effective to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the percentage of identity is determined over an alignment region of at least 200 nucleotides.
[00157] [00157] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant, comprising disposing, in a heterologous manner, of an endophyte organism for an element of the soybean plant in an effective amount to increase a trait of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the percentage of identity is determined along a hair alignment region minus 300 nucleotides.
[00158] [00158] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate,
[00159] [00159] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from an element of the reference plant, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, and in which the percentage of identity is determined over an alignment region of at least 300 nucleotides.
[00160] [00160] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the soybean plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus It curves and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined along an alignment region. at least 300 nucleotides.
[00161] [00161] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 34-38, where the percentage of identity is determined over an alignment region of at least 300 nucleot deos.
[00162] [00162] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Aspergillus and comprises at least one polynucleotic sequence at least 97% identical to SEQ ID NO: 39, in which the percentage of identity is determined over an alignment region of at least 300 nucleotides.
[00163] [00163] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, root length, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the organism endophyte is a member of the genus Coniochaeta and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 4043, in which the percentage of identity is determined over a alignment region of at least 300 nucleotides.
[00164] [00164] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the rice plant in an amount effective to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over an alignment region of at least 300 nucleotides.
[00165] [00165] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate and dry biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over an alignment region of at least 300 nucleotides.
[00166] [00166] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the rice plant in an amount effective to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the percentage of identity is determined over an alignment region of at least 300 nucleotides.
[00167] [00167] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant, comprising disposing, in a heterologous manner, of an endophyte organism for an element of the soybean plant in an effective amount to increase a trait of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the percentage of identity is determined along a hair alignment region minus 400 nucleotides.
[00168] [00168] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the percentage of identity is determined along an alignment region of at least 400 nucleotides.
[00169] [00169] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an effective amount to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endáophyte organism is a member of the genus Periconia and comprises at least one polynucleot tip sequence at least 97% identical to a polynucleot tip sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the percentage of identity is determined over an alignment region of at least 400 nucleotides.
[00170] [00170] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the soybean plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus It curves and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined along a region of hair alignment. minus 400 nucleotides.
[00171] [00171] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 34-38, where the percentage of identity is determined over an alignment region of at least 400 nucleot deos.
[00172] [00172] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from an element of the reference plant, in which the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleotic sequence at least 97% identical to SEQ ID NO : 39, where the percentage of identity is determined over an alignment region of at least 400 nucleotides.
[00173] [00173] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, root length, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the organism endophyte is a member of the genus Coniochaeta and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 4043, in which the percentage of identity is determined over a alignment region of at least 400 nucleotides.
[00174] [00174] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the rice plant in an effective amount to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot hint at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over an alignment region of at least 400 nucleotides.
[00175] [00175] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate and dry biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over an alignment region of at least 400 nucleotides.
[00176] [00176] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the rice plant in an amount effective to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Enterobacter and comprises at least one polynucleotic sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the percentage of identity is determined over an alignment region of at least 400 nucleotides.
[00177] [00177] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant, comprising disposing, in a heterologous manner, of an endophyte organism for an element of the soybean plant in an effective amount to increase a trait of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the percentage of identity is determined along a hair alignment region minus 500 nucleotides.
[00178] [00178] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33,
[00179] [00179] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a corn plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the corn plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from an element of the reference plant, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, and in which the percentage of identity is determined over an alignment region of at least 500 nucleotides.
[00180] [00180] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a soybean plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the soybean plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus It curves and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined along a region of hair alignment. minus 500 nucleotides.
[00181] [00181] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of root length and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 34-38, where the percentage of identity is determined over an alignment region of at least 500 nucleot deos.
[00182] [00182] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase the yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte is a member of the genus Aspergillus and comprises at least one polynucleotic sequence at least 97% identical to SEQ ID NO: 39, in which the percentage of identity is determined over an alignment region of at least 500 nucleotides.
[00183] [00183] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate, root length, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the organism endophyte is a member of the genus Coniochaeta and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 4043, in which the percentage of identity is determined over a alignment region of at least 500 nucleotides.
[00184] [00184] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism for an element of the rice plant in an effective amount to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over an alignment region of at least 500 nucleotides.
[00185] [00185] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a wheat plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the wheat plant in an amount effective to increase a trait of agronomic importance selected from the group consisting of germination rate and dry biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over an alignment region of at least 500 nucleotides.
[00186] [00186] In one aspect, the invention provides a method of enhancing a trait of agronomic importance in a rice plant comprising disposing, in a heterologous manner, of an endophyte organism to an element of the rice plant in an amount effective to increase the dry root biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the percentage of identity is determined over an alignment region of at least 500 nucleotides.
[00187] [00187] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 32 and 33.
[00188] [00188] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant,
[00189] [00189] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67466.
[00190] [00190] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38.
[00191] [00191] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endáophytic organism of the genus Curvularia, as deposited under the NRRL Culture Deposit No. 67467.
[00192] [00192] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant, polymer and an active microbial ingredient, in which the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67467.
[00193] [00193] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Aspergillus genus and comprises at least one polynucleot sequence hints at least 97% identical to SEQ ID NO: 39.
[00194] [00194] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Coniochaeta and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 40-43.
[00195] [00195] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Enterobacter genus and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 49-51.
[00196] [00196] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophytic organism of the genus Enterobacter, as deposited under the NRRL Culture Deposit No. B67465.
[00197] [00197] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under NRRL Culture Deposit No. B67465, in which the oil is rapeseed or NEEM.
[00198] [00198] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophytic organism of the genus Periconia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the oil is rapeseed or NEEM.
[00199] [00199] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the microbial active ingredient is an endúafafito organism of the genus Periconia, as deposited under the NRRL Culture Deposit No. 67466, in which the oil is rapeseed or NEEM.
[00200] [00200] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant,
[00201] [00201] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia and comprises at least one hint polynucleot sequence at least 97% identical to a hint polynucleot sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the oil is rapeseed or NEEM.
[00202] [00202] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophytic organism of the genus Curvularia, as deposited under the NRRL Culture Deposit No. 67467, in which the oil is rapeseed or NEEM.
[00203] [00203] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67467, in which the oil is rapeseed or NEEM.
[00204] [00204] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Aspergillus genus and comprises at least one polynucleot sequence hints at least 97% identical to SEQ ID NO: 39, in which the oil is rapeseed or NEEM.
[00205] [00205] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Coniochaeta and comprises at least one polynucleot hint sequence at least 97% identical to a hint polynucleot sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the oil is rapeseed or NEEM.
[00206] [00206] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Enterobacter genus and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the oil is rapeseed or NEEM.
[00207] [00207] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Enterobacter, as deposited under the NRRL Culture Deposit No. B67465, in which the oil is rapeseed or NEEM.
[00208] [00208] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under NRRL Culture Deposit No. B67465, in which the oil is rapeseed or NEEM.
[00209] [00209] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the oil comprises erucic acid.
[00210] [00210] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia, as deposited under the NRRL Culture Deposit No. 67466, in which the oil comprises erucic acid.
[00211] [00211] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67466, in which the oil comprises erucic acid.
[00212] [00212] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the oil comprises erucic acid.
[00213] [00213] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia, as deposited under the NRRL Culture Deposit No. 67467, in which the oil comprises erucic acid.
[00214] [00214] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67467, in which the oil comprises erucic acid.
[00215] [00215] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Aspergillus and comprises at least one polynucleot sequence at least 97% identical to SEQ ID NO: 39, in which the oil comprises erucic acid.
[00216] [00216] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Coniochaeta and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the oil comprises erucic acid.
[00217] [00217] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Enterobacter genus and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the oil comprises erucic acid.
[00218] [00218] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Enterobacter, as deposited under the NRRL Culture Deposit No. B67465, in which the oil comprises erucic acid.
[00219] [00219] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under NRRL Culture Deposit No. B67465, in which the oil comprises erucic acid.
[00220] [00220] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the oil comprises herbicidal or insecticidal properties.
[00221] [00221] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia, as deposited under the NRRL Culture Deposit No. 67466, in which the oil comprises herbicidal or insecticidal properties.
[00222] [00222] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67466, in which the oil comprises herbicidal or insecticidal properties.
[00223] [00223] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the oil comprises herbicidal or insecticidal properties.
[00224] [00224] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endáophytic organism of the genus Curvularia, as deposited under the NRRL Culture Deposit No. 67467, in which the oil comprises herbicidal or insecticidal properties.
[00225] [00225] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67467, in which the oil comprises herbicidal or insecticidal properties.
[00226] [00226] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Aspergillus genus and comprises at least one polynucleot sequence at least 97% identical to SEQ ID NO: 39, in which the oil comprises herbicidal or insecticidal properties.
[00227] [00227] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Coniochaeta and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the oil comprises herbicidal or insecticidal properties.
[00228] [00228] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Enterobacter genus and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the oil comprises herbicidal or insecticidal properties.
[00229] [00229] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Enterobacter, as deposited under the NRRL Culture Deposit No. B67465, in which the oil comprises herbicidal or insecticidal properties.
[00230] [00230] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under NRRL Culture Deposit No. B67465, in which the oil comprises herbicidal or insecticidal properties.
[00231] [00231] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophytic organism of the genus Periconia and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the surfactant is a non-ionic detergent.
[00232] [00232] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endemic organism of the genus Periconia, as deposited under the NRRL Culture Deposit No. 67466, in which the surfactant is a non-ionic detergent.
[00233] [00233] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67466, where the surfactant is a non-ionic detergent.
[00234] [00234] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the surfactant is a non-ionic detergent.
[00235] [00235] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia, as deposited under the NRRL Culture Deposit No. 67467, in which the surfactant is a non-ionic detergent.
[00236] [00236] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67467, in which the surfactant is a non-ionic detergent.
[00237] [00237] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Aspergillus genus and comprises at least one polynucleot sequence hints at least 97% identical to SEQ ID NO: 39, in which the surfactant is a non-ionic detergent.
[00238] [00238] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophytic organism of the genus Coniochaeta and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the surfactant is a non-ionic detergent.
[00239] [00239] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Enterobacter and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the surfactant is a non-ionic detergent.
[00240] [00240] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Enterobacter, as deposited under the NRRL Culture Deposit No. B67465, in which the surfactant is a non-ionic detergent.
[00241] [00241] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under NRRL Culture Deposit No. B67465, in which the surfactant is a non-ionic detergent.
[00242] [00242] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, where the surfactant is Tween 20 or Triton X-100.
[00243] [00243] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the microbial active ingredient is an endúafafito organism of the genus Periconia, as deposited under the NRRL Culture Deposit No. 67466, where the surfactant is Tween 20 or Triton X-100.
[00244] [00244] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67466, where the surfactant is Tween 20 or Triton X-100.
[00245] [00245] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 34-38, where the surfactant is Tween 20 or Triton X-100.
[00246] [00246] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia, as deposited under the NRRL Culture Deposit No. 67467, where the surfactant is Tween 20 or Triton X-100.
[00247] [00247] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67467, where the surfactant is Tween 20 or Triton X-100.
[00248] [00248] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Aspergillus and comprises at least one polynucleot sequence at least 97% identical to SEQ ID NO: 39, where the surfactant is Tween 20 or Triton X-100.
[00249] [00249] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Coniochaeta and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 40-43, where the surfactant is Tween 20 or Triton X-100.
[00250] [00250] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Enterobacter genus and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 49-51, where the surfactant is Tween 20 or Triton X-100.
[00251] [00251] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Enterobacter, as deposited under the NRRL Culture Deposit No. B67465, where the surfactant is Tween 20 or Triton X-100.
[00252] [00252] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under NRRL Culture Deposit No. B67465, where the surfactant is Tween 20 or Triton X-100.
[00253] [00253] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one oil, surfactant,
[00254] [00254] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endemic organism of the genus Periconia, as deposited under the NRRL Culture Deposit No. 67466, where the polymer is Flo RiteG &, DISCOTM or KannarO Universal Wonder.
[00255] [00255] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67466, where the polymer is Flo Rite &, DISCOTM or Kannar & Universal Wonder.
[00256] [00256] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleot cue sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the polymer is Flo Rite &, DISCOTM or Kannar & Universal Wonder.
[00257] [00257] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the microbial active ingredient is an endophyte organism of the genus Curvularia, as deposited under the NRRL Culture Deposit No. 67467, where the polymer is Flo RiteG & G, DISCOTM or KannarO Universal Wonder.
[00258] [00258] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67467, where the polymer is Flo Rite &, DISCOTM or Kannar & Universal Wonder.
[00259] [00259] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Aspergillus genus and comprises at least one polynucleot sequence hints at least 97% identical to SEQ ID NO: 39, where the polymer is Flo Rite &, DISCOTM or Kannar & Universal Wonder.
[00260] [00260] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Coniochaeta and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotic sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the polymer is Flo Rite &, DISCOTM or Kannar & 6 Universal Wonder.
[00261] [00261] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Enterobacter and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotic sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the polymer is Flo Rite &, DISCOTM or Kannar & Universal Wonder.
[00262] [00262] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Enterobacter, as deposited under the NRRL Culture Deposit No. B67465, where the polymer is Flo Rite €, DISCOTM or Kannar & Universal Wonder.
[00263] [00263] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under NRRL Culture Deposit No. B67465, where the polymer is Flo Rite &, DISCOTM or Kannar & Universal Wonder.
[00264] [00264] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the microbial active ingredient comprises a spore suspension.
[00265] [00265] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the microbial active ingredient is an endogenous organism of the genus Periconia, as deposited under the NRRL Culture Deposit No. 67466, in which the microbial active ingredient comprises a spore suspension.
[00266] [00266] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67466, in which the microbial active ingredient comprises a spore suspension.
[00267] [00267] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the microbial active ingredient comprises a spore suspension.
[00268] [00268] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia, as deposited under the NRRL Culture Deposit No. 67467, in which the microbial active ingredient comprises a spore suspension.
[00269] [00269] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67467, in which the microbial active ingredient comprises a spore suspension.
[00270] [00270] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Aspergillus genus and comprises at least one polynucleot sequence tips at least 97% identical to SEQ ID NO: 39, in which the microbial active ingredient comprises a spore suspension.
[00271] [00271] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Coniochaeta and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the microbial active ingredient comprises a spore suspension.
[00272] [00272] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Enterobacter genus and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the microbial active ingredient comprises a spore suspension.
[00273] [00273] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Enterobacter, as deposited under the NRRL Culture Deposit No. B67465, in which the microbial active ingredient comprises a spore suspension.
[00274] [00274] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under NRRL Culture Deposit No. B67465, in which the microbial active ingredient comprises a spore suspension.
[00275] [00275] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the microbial active ingredient comprises spray dried spores.
[00276] [00276] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia, as deposited under the NRRL Culture Deposit No. 67466, in which the microbial active ingredient comprises spray dried spores.
[00277] [00277] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant, polymer and an active microbial ingredient, in which the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67466, in which the microbial active ingredient comprises spray dried spores.
[00278] [00278] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the microbial active ingredient comprises spray dried spores.
[00279] [00279] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia, as deposited under the NRRL Culture Deposit No. 67467, in which the microbial active ingredient comprises spray dried spores.
[00280] [00280] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67467, where the microbial active ingredient comprises spray dried spores.
[00281] [00281] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Aspergillus genus and comprises at least one polynucleot sequence at least 97% identical to SEQ ID NO: 39, where the microbial active ingredient comprises spray dried spores.
[00282] [00282] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophytic organism of the genus Coniochaeta and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the microbial active ingredient comprises spray dried spores.
[00283] [00283] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Enterobacter genus and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 49-51,
[00284] [00284] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endáophytic organism of the genus Enterobacter, as deposited under the NRRL Culture Deposit No. B67465, in which the microbial active ingredient comprises spray dried spores.
[00285] [00285] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under NRRL Culture Deposit No. B67465, in which the microbial active ingredient comprises spray-dried spores.
[00286] [00286] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the microbial active ingredient comprises whole cell broth.
[00287] [00287] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endemic organism of the genus Periconia, as deposited under the NRRL Culture Deposit No. 67466, in which the microbial active ingredient comprises whole cell broth.
[00288] [00288] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67466, in which the microbial active ingredient comprises whole cell broth.
[00289] [00289] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the microbial active ingredient comprises whole cell broth.
[00290] [00290] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endáophyte organism of the genus Curvularia, as deposited under the NRRL Culture Deposit No. 67467, in which the microbial active ingredient comprises whole cell broth.
[00291] [00291] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67467, in which the microbial active ingredient comprises whole cell broth.
[00292] [00292] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Aspergillus genus and comprises at least one polynucleot sequence hints at least 97% identical to SEQ ID NO: 39, in which the microbial active ingredient comprises whole cell broth.
[00293] [00293] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Coniochaeta and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the microbial active ingredient comprises whole cell broth.
[00294] [00294] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Enterobacter and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the microbial active ingredient comprises whole cell broth.
[00295] [00295] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophytic organism of the genus Enterobacter, as deposited under the NRRL Culture Deposit No. B67465, in which the microbial active ingredient comprises whole cell broth.
[00296] [00296] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under NRRL Culture Deposit No. B67465, in which the microbial active ingredient comprises whole cell broth.
[00297] [00297] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 32 and 33, the formulation further comprising one of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
[00298] [00298] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia, as deposited under the NRRL Culture Deposit No. 67466, the formulation further comprising one of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
[00299] [00299] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67466, the formulation further comprising one of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
[00300] [00300] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, the formulation further comprising one of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
[00301] [00301] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia, as deposited under the NRRL Culture Deposit No. 67467, the formulation further comprising one of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
[00302] [00302] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67467, the formulation further comprising one of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
[00303] [00303] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Aspergillus genus and comprises at least one polynucleot sequence at least 97% identical to SEQ ID NO: 39, the formulation further comprising one of the following: fungicide, nematicide,
[00304] [00304] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophytic organism of the genus Coniochaeta and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 40-43, the formulation further comprising one of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
[00305] [00305] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Enterobacter and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 49-51, the formulation further comprising one of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
[00306] [00306] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Enterobacter, as deposited under the NRRL Culture Deposit No. B67465, the formulation further comprising one of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
[00307] [00307] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under NRRL Culture Deposit No. B67465, the formulation further comprising one of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
[00308] [00308] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 32 and 33, the formulation further comprising one of the following: stabilizer, preservative, carrier, anti-complex agent or any combination thereof.
[00309] [00309] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia, as deposited under the NRRL Culture Deposit No. 67466, the formulation further comprising one of the following: stabilizer, preservative, carrier, anti-complex agent or any combination thereof.
[00310] [00310] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67466, the formulation further comprising one of the following: stabilizer, preservative, carrier, anti-complex agent or any combination thereof.
[00311] [00311] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, the formulation further comprising one of the following: stabilizer, preservative, carrier, anti-complex agent or any combination thereof.
[00312] [00312] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endáophytic organism of the genus Curvularia, as deposited under the NRRL Culture Deposit No. 67467, the formulation further comprising one of the following: stabilizer, preservative, carrier, anti-complex agent or any combination thereof.
[00313] [00313] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67467, the formulation further comprising one of the following: stabilizer, preservative, carrier, anti-complex agent or any combination thereof.
[00314] [00314] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Aspergillus genus and comprises at least one polynucleot sequence at least 97% identical to SEQ ID NO: 39, the formulation further comprising one of the following: stabilizer, preservative, carrier, anti-complex agent or any combination thereof.
[00315] [00315] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Coniochaeta and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 40-43, the formulation further comprising one of the following: stabilizer, preservative, carrier, anti-complex agent or any combination thereof.
[00316] [00316] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Enterobacter genus and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 49-51, the formulation further comprising one of the following: stabilizer, preservative, carrier, anti-complex agent or any combination thereof.
[00317] [00317] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant,
[00318] [00318] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under NRRL Culture Deposit No. B67465, the formulation further comprising one of the following: stabilizer, preservative, carrier, anti-complex agent or any combination thereof.
[00319] [00319] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophytic organism of the genus Periconia and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the endophyte organism is self-stable.
[00320] [00320] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the microbial active ingredient is an endogenous organism of the genus Periconia, as deposited under the NRRL Culture Deposit No. 67466, in which the endophyte organism is self-stable.
[00321] [00321] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67466, in which the endophyte organism is self-stable.
[00322] [00322] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the endophyte organism is self-stable.
[00323] [00323] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia, as deposited under the NRRL Culture Deposit No. 67467, in which the endophyte organism is self-stable.
[00324] [00324] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67467, in which the endophyte organism is self-stable.
[00325] [00325] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Aspergillus genus and comprises at least one polynucleot sequence hints at least 97% identical to SEQ ID NO: 39, in which the endophyte organism is self-stable.
[00326] [00326] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophytic organism of the genus Coniochaeta and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the endophyte organism is self-stable.
[00327] [00327] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Enterobacter genus and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the endophyte organism is self-stable.
[00328] [00328] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Enterobacter, as deposited under the NRRL Culture Deposit No. B67465, in which the endophyte organism is self-stable.
[00329] [00329] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under NRRL Culture Deposit No. B67465, in which the endophyte organism is self-stable.
[00330] [00330] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 32 and 33, where the percentage of identity is at least 98%.
[00331] [00331] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, where the percentage of identity is at least 98%.
[00332] [00332] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Aspergillus genus and comprises at least one polynucleot sequence hints at least 97% identical to SEQ ID NO: 39, where the percentage of identity is at least 98%.
[00333] [00333] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Coniochaeta and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 40-43, where the percentage of identity is at least 98%.
[00334] [00334] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Enterobacter and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 49-51, where the percentage of identity is at least 98%.
[00335] [00335] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the percentage of identity is at least 99%.
[00336] [00336] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, where the percentage of identity is at least 99%.
[00337] [00337] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Aspergillus genus and comprises at least one polynucleot sequence hints at least 97% identical to SEQ ID NO: 39, where the percentage of identity is at least 99%.
[00338] [00338] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Coniochaeta and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 40-43, where the percentage of identity is at least 99%.
[00339] [00339] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Enterobacter genus and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 49-51, where the percentage of identity is at least 99%.
[00340] [00340] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 32 and 33, where the percentage of identity is 100%.
[00341] [00341] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, where the percentage of identity is 100%.
[00342] [00342] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant,
[00343] [00343] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophytic organism of the genus Coniochaeta and comprises at least one polynucleot hint sequence at least 97% identical to a hint polynucleot sequence selected from the group consisting of SEQ ID NOs: 40-43, where the percentage of identity is 100%.
[00344] [00344] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Enterobacter and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 49-51, where the percentage of identity is 100%.
[00345] [00345] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the percentage of identity is determined over an alignment region of at least 100 nucleotides.
[00346] [00346] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over an alignment region of at least 100 nucleotides.
[00347] [00347] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Aspergillus genus and comprises at least one polynucleot sequence hints at least 97% identical to SEQ ID NO: 39, in which the percentage of identity is determined over an alignment region of at least 100 nucleotides.
[00348] [00348] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophytic organism of the genus Coniochaeta and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over an alignment region of at least 100 nucleotides.
[00349] [00349] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Enterobacter and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the percentage of identity is determined over an alignment region of at least 100 nucleotides.
[00350] [00350] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the percentage of identity is determined over an alignment region of at least 200 nucleotides.
[00351] [00351] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over an alignment region of at least 200 nucleotides.
[00352] [00352] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Aspergillus genus and comprises at least one polynucleot sequence hints at least 97% identical to SEQ ID NO: 39, in which the percentage of identity is determined over an alignment region of at least 200 nucleotides.
[00353] [00353] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophytic organism of the genus Coniochaeta and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over an alignment region of at least 200 nucleotides.
[00354] [00354] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Enterobacter genus and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the percentage of identity is determined over an alignment region of at least 200 nucleotides.
[00355] [00355] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the percentage of identity is determined over an alignment region of at least 300 nucleotides.
[00356] [00356] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over an alignment region of at least 300 nucleotides.
[00357] [00357] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Aspergillus genus and comprises at least one polynucleot sequence hints at least 97% identical to SEQ ID NO: 39, where the percent identity is determined over an alignment region of at least 300 nucleotides.
[00358] [00358] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Coniochaeta and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over an alignment region of at least 300 nucleotides.
[00359] [00359] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Enterobacter genus and comprises at least one polynucleot hint sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the percentage of identity is determined over an alignment region of at least 300 nucleotides.
[00360] [00360] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the percentage of identity is determined over an alignment region of at least 400 nucleotides.
[00361] [00361] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over an alignment region of at least 400 nucleotides.
[00362] [00362] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Aspergillus genus and comprises at least one polynucleot sequence hints at least 97% identical to SEQ ID NO: 39, in which the percentage of identity is determined over an alignment region of at least 400 nucleotides.
[00363] [00363] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Coniochaeta and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over an alignment region of at least 400 nucleotides.
[00364] [00364] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Enterobacter genus and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the percent identity is determined over an alignment region of at least 400 nucleotides.
[00365] [00365] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Periconia and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the percentage of identity is determined over an alignment region of at least 500 nucleotides.
[00366] [00366] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Curvularia and comprises at least one polynucleotyl sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over an alignment region of at least 500 nucleotides.
[00367] [00367] In one aspect, the invention provides an agrochemically active microbial formulation comprising at least one oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the Aspergillus genus and comprises at least one polynucleot sequence hints at least 97% identical to SEQ ID NO: 39, in which the percentage of identity is determined over an alignment region of at least 500 nucleotides.
[00368] [00368] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Coniochaeta and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over an alignment region of at least 500 nucleotides.
[00369] [00369] In one aspect, the invention provides an agro-chemically active microbial formulation comprising at least one Oil, surfactant, polymer and a microbial active ingredient, wherein the microbial active ingredient is an endophyte organism of the genus Enterobacter and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the percentage of identity is determined over an alignment region of at least 500 nucleotides.
[00370] [00370] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte organism.
[00371] [00371] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to each of the sequences within the group consisting of SEQs
[00372] [00372] In one aspect, the invention provides a synthetic composition that comprises a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466 , in which the synthetic composition is capable of providing an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism.
[00373] [00373] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under Deposit NRRL Culture No. 67466, in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism.
[00374] [00374] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism belongs to the Periconia macrospinosa taxonomy.
[00375] [00375] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to each of the sequences within the group consisting of SEQ ID NO: 32 and 33, in which the endophyte organism belongs to the Periconia macrospinosa taxonomy.
[00376] [00376] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism belongs to the Periconia macrospinosa taxonomy.
[00377] [00377] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under Deposit of NRRL Culture No. 67466, in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the endophyte organism is from the Periconia macrospinosa taxonomy .
[00378] [00378] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte organism, in which the plant element is of the genus Triticum and the enhanced trait of agronomic importance is selected from the group consisting of germination rate, dry shoot biomass and yield.
[00379] [00379] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleot sequences identical to each of the sequences within the group consisting of SEQ ID NO: 32 and 33, in which the plant element is of the genus Triticum and the enhanced trait of agronomic importance is selected from the group consisting of germination rate, dry shoot biomass and yield.
[00380] [00380] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, that the element of the plant is of the genus Triticum and the enhanced trait of agronomic importance is selected from the group consisting of germination rate, dry shoot biomass and yield.
[00381] [00381] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under Deposit NRRL Culture No. 67466, in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, that the plant element is of the genus Triticum and the improved trait of agronomic importance is selected from the group consisting of germination rate, dry shoot biomass and yield.
[00382] [00382] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte organism, in which the plant element is of the genus Glycine and the enhanced trait of agronomic importance is selected from the group consisting of root length and root area.
[00383] [00383] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotine sequences identical to each of the sequences within the group consisting of SEQ ID NO: 32 and 33, in which the plant element is of the genus Glycine and the enhanced trait of agronomic importance is selected from the group consisting of root length and root area.
[00384] [00384] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the element of the plant is of the genus Glycine and the enhanced trait of agronomic importance is selected from the group consisting of the root length and root area.
[00385] [00385] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under Deposit NRRL Culture No. 67466, in which the synthetic composition is able to provide an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the plant element is of the genus Glycine and the enhanced trait of agronomic importance is selected from the group consisting of root length and root area.
[00386] [00386] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte organism, in which the plant element is of the genus Zea and the improved trait of agronomic importance is yield.
[00387] [00387] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% identical polynucleot sequences to each of the sequences within the group consisting of SEQ ID NO: 32 and 33, in which the plant element is of the genus Zea and the enhanced trait of agronomic importance is yield.
[00388] [00388] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, in which the endophyte organism is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the element of the plant is of the genus Zea and the enhanced trait of agronomic importance it's income.
[00389] [00389] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under Deposit NRRL Culture No. 67466, in which the synthetic composition is able to provide an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the plant element is of the genus Zea and the improved trait of agronomic importance is yield.
[00390] [00390] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism.
[00391] [00391] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophytic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 34-38.
[00392] [00392] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophobic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophytic organism, in which the endophytic organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 34-38.
[00393] [00393] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least four sequences selected from the group consisting of SEQ ID NOs: 34-38.
[00394] [00394] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least five sequences selected from the group consisting of SEQ ID NOs: 34-38.
[00395] [00395] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467 , in which the synthetic composition is capable of providing an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism.
[00396] [00396] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under Deposit NRRL Culture No. 67467, in which the synthetic composition is able to provide an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism.
[00397] [00397] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the referred endophyte organism is from the Curvularia spicifera taxonomy.
[00398] [00398] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the endophyte organism referred to is from the Curvularia spicifera taxonomy.
[00399] [00399] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the referred endophyte organism is from the Curvularia spicifera taxonomy.
[00400] [00400] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophobic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least four sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the referred endophyte organism is from the Curvularia spicifera taxonomy.
[00401] [00401] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-
[00402] [00402] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467 , in which the synthetic composition is capable of providing an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the referred endophyte organism is from the Curvularia spicifera taxonomy.
[00403] [00403] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under Deposit of NRRL Culture No. 67467, in which the synthetic composition is able to provide an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the referred endophyte organism is of the Curvular taxonomy spicifera.
[00404] [00404] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the plant element is of the genus Triticum and the enhanced trait of agronomic importance is selected from the group consisting of root length and yield.
[00405] [00405] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the plant element is of the genus Triticum and the trait crop of agronomic importance is selected from the group consisting of root length and yield.
[00406] [00406] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleot sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the plant element is of the genus Triticum and the trait crop of agronomic importance is selected from the group consisting of root length and yield.
[00407] [00407] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-
[00408] [00408] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleot sequences identical to at least five sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the plant element is of the genus Triticum and the trait crop of agronomic importance is selected from the group consisting of root length and yield.
[00409] [00409] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, that the element of the plant is of the genus Triticum and the enhanced trait of agronomic importance is selected from the group consisting of root length and yield.
[00410] [00410] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under Deposit NRRL Culture No. 67467, in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, that the plant element is of the genus Triticum and the enhanced trait of agronomic importance is selected from the group consisting of root length and yield.
[00411] [00411] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the plant element is of the genus Glycine and the enhanced trait of agronomic importance is selected from the group consisting of root length, germination rate and yield.
[00412] [00412] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleot sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the plant element is of the genus Glycine and the trait crop of agronomic importance is selected from the group consisting of root length, germination rate and yield.
[00413] [00413] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleot sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the plant element is of the genus Glycine and the trait crop of agronomic importance is selected from the group consisting of root length, germination rate and yield.
[00414] [00414] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleot sequences identical to at least four sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the plant element is of the genus Glycine and the trait crop of agronomic importance is selected from the group consisting of root length, germination rate and yield.
[00415] [00415] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleot hint sequences
[00416] [00416] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467 , where the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, that the element of the plant is of the genus Glycine and the enhanced trait of agronomic importance is selected from the group consisting of root length, germination rate and yield.
[00417] [00417] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under Deposit NRRL Culture No. 67467, in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, that the plant element is of the genus Glycine and the enhanced trait of agronomic importance is selected from the group consisting of root length, germination rate and yield.
[00418] [00418] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the plant element is of the genus Zea and the improved trait of agronomic importance is yield.
[00419] [00419] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophobic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the plant element is of the genus Zea and the trait improved agronomic importance is yield.
[00420] [00420] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleot sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the plant element is of the genus Zea and the trait improved agronomic importance is yield.
[00421] [00421] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleot sequences identical to at least four sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the plant element is of the genus Zea and the trait improved agronomic importance is yield.
[00422] [00422] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleot sequences identical to at least five sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the plant element is of the genus Zea and the trait improved agronomic importance is yield.
[00423] [00423] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the element of the plant is of the genus Zea and the enhanced trait of agronomic importance it's income.
[00424] [00424] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under Deposit NRRL Culture No. 67467, in which the synthetic composition is able to provide an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the plant element is of the genus Zea and the improved trait of agronomic importance is yield.
[00425] [00425] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleotic sequence at least 97 % identical to SEQ ID NO: 39, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophyte organism.
[00426] [00426] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleot sequence at least 97 % identical to SEQ ID NO: 39, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the said endophyte organism belongs to Aspergillus ruber taxonomy.
[00427] [00427] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleot sequence at least 97 % identical to SEQ ID NO: 39, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophyte organism, in which the plant element is the genus Triticum and the improved trait of agronomic importance is yield.
[00428] [00428] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleot sequence at least 97 % identical to SEQ ID NO: 39, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the said endophyte organism belongs to taxonomy Aspergillus ruber, that the plant element is of the genus Triticum and the improved trait of agronomic importance is yield.
[00429] [00429] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endúophyte organism.
[00430] [00430] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 40-43.
[00431] [00431] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 40-43.
[00432] [00432] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least four sequences selected from the group consisting of SEQ ID NOs: 40-43.
[00433] [00433] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism belongs to the Coniochaeta prunicola taxonomy.
[00434] [00434] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the endophyte organism belongs to the Coniochaeta prunicola taxonomy.
[00435] [00435] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophobic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the endophyte organism is from the Coniochaeta prunicola taxonomy.
[00436] [00436] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least four sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the endophyte organism belongs to the Coniochaeta prunicola taxonomy.
[00437] [00437] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the plant element is of the genus Triticum and the enhanced trait of agronomic importance is selected from the group consisting of root length, germination rate, dry shoot biomass and yield.
[00438] [00438] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleot sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the plant element is of the genus Triticum and the trait crop of agronomic importance is selected from the group consisting of root length, germination rate, dry shoot biomass and yield.
[00439] [00439] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleot sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the plant element is of the genus Triticum and the trait crop of agronomic importance is selected from the group consisting of root length, germination rate, dry shoot biomass and yield.
[00440] [00440] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleot sequences identical to at least four sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the plant element is of the genus Triticum and the trait crop of agronomic importance is selected from the group consisting of root length, germination rate, dry shoot biomass and yield O.
[00441] [00441] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophytic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the plant element is of the Oryza genus and the enhanced trait of agronomic importance is dry root biomass.
[00442] [00442] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, in which the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleot sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the plant element is of the genus Oryza and the trait of agronomic importance is dry root biomass.
[00443] [00443] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleot sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the plant element is of the genus Oryza and the trait of agronomic importance is dry root biomass.
[00444] [00444] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleot sequences identical to at least four sequences selected from the group consisting of SEQ ID Nos: 4043, in which the plant element is of the genus Oryza and the enhanced trait of importance agronomic is the dry root biomass.
[00445] [00445] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiops and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endúafafito organism.
[00446] [00446] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that it does not further understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 44-48.
[00447] [00447] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiops and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 44-48.
[00448] [00448] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiops and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least four sequences selected from the group consisting of SEQ ID NOs: 44-48.
[00449] [00449] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiops and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that it does not further understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least five sequences selected from the group consisting of SEQ ID NOs: 44-48.
[00450] [00450] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiops and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism belongs to the Pestalotiopsis neglecta taxonomy.
[00451] [00451] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiops and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the endophyte organism is from the Pestalotiopsis neglecta taxonomy.
[00452] [00452] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiops and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44-
[00453] [00453] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiops and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least four sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the endophyte organism is from the Pestalotiopsis neglecta taxonomy.
[00454] [00454] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiops and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least five sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the endophyte organism is from the Pestalotiopsis neglecta taxonomy.
[00455] [00455] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the plant element is of the genus Triticum and the enhanced trait of agronomic importance is selected from the group consisting of germination rate and dry shoot biomass.
[00456] [00456] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiops and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleot sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the plant element is of the genus Triticum and the enhanced trait of agronomic importance is selected from the group consisting of germination rate and dry shoot biomass.
[00457] [00457] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleot sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the plant element is of the genus Triticum and the enhanced trait of agronomic importance is selected from the group consisting of germination rate and dry shoot biomass.
[00458] [00458] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophytic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleot sequences identical to at least four sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the plant element is of the genus Triticum and the enhanced trait of agronomic importance is selected from the group consisting of germination rate and dry shoot biomass.
[00459] [00459] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophytic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleot sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the plant element is of the genus Triticum and the enhanced trait of agronomic importance is selected from the group consisting of germination rate and dry shoot biomass.
[00460] [00460] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the Enterobacter genus and comprises at least one polynucleotide sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49-
[00461] [00461] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the Enterobacter genus and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 49-51.
[00462] [00462] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, wherein the endophyte organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 49-51.
[00463] [00463] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter, as deposited under NRRL Culture Deposit No. B67465 , in which the synthetic composition is capable of providing an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism.
[00464] [00464] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under Deposit and NRRL culture No. B67465, in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism.
[00465] [00465] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the Enterobacter genus and comprises at least one polynucleotide sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which said endophyte organism is from the Enterobacter cowanii taxonomy.
[00466] [00466] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 49-51, in which said endophyte organism is from the Enterobacter cowanii taxonomy.
[00467] [00467] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49-
[00468] [00468] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter, as deposited under NRRL Culture Deposit No. B67465 , in which the synthetic composition is capable of providing an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the referred endophyte organism is from the Enterobacter cowanii taxonomy.
[00469] [00469] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under Deposit and NRRL culture No. B67465, in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which said endophyte organism is from the Enterobacter cowanii taxonomy .
[00470] [00470] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the plant element is of the genus Oryza and the improved trait of agronomic importance is the dry root biomass.
[00471] [00471] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the Enterobacter genus and comprises at least one polynucleotide sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% identical polynucleot sequences to at least two sequences selected from the group consisting of SEQ ID NOs: 49-51, in which the plant element is of the genus Oryza and the enhanced trait of agronomic importance is the dry root biomass.
[00472] [00472] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the endophyte organism comprises at least 97% polynucleot sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 49-51, in which the plant element is of the genus Oryza and the enhanced trait of agronomic importance is the dry root biomass.
[00473] [00473] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter, as deposited under NRRL Culture Deposit No. B67465 , in which the synthetic composition is capable of providing an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the element of the plant is of the genus Oryza and the enhanced trait of agronomic importance is the dry root biomass.
[00474] [00474] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under Deposit and NRRL culture No. B67465, in which the synthetic composition is able to provide an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the plant element is of the genus Oryza and the improved trait of agronomic importance is the dry root biomass.
[00475] [00475] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte organism, in which the improved trait of agronomic importance is conferred under normal irrigation conditions.
[00476] [00476] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, in which the endophyte organism is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466 , in which the synthetic composition is capable of providing an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the referred trait of agronomic importance is conferred under normal irrigation conditions.
[00477] [00477] In one aspect, the invention provides a synthetic composition that comprises a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under Deposit NRRL Culture No. 67466, in which the synthetic composition is capable of providing an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the said enhanced trait of agronomic importance is under normal watering conditions.
[00478] [00478] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the improved trait of agronomic importance is conferred under normal irrigation conditions.
[00479] [00479] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the referred trait of agronomic importance is conferred under normal irrigation conditions.
[00480] [00480] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under Deposit of NRRL Culture No. 67467, in which the synthetic composition is able to provide an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which said enhanced trait of agronomic importance is conferred under normal watering conditions.
[00481] [00481] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleot sequence at least 97 % identical to SEQ ID NO: 39, in which the said synthetic composition is capable of providing an enhanced trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophyte organism, in which the said enhanced trait of importance agronomy is provided under normal irrigation conditions.
[00482] [00482] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the improved trait of agronomic importance is conferred under normal irrigation conditions.
[00483] [00483] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiops and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the improved trait of agronomic importance is conferred under normal irrigation conditions.
[00484] [00484] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophobic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the improved trait of agronomic importance is conferred under normal irrigation conditions.
[00485] [00485] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter, as deposited under NRRL Culture Deposit No. B67465 , in which the synthetic composition is capable of providing an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the referred trait of agronomic importance is conferred under normal irrigation conditions.
[00486] [00486] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under Deposit and NRRL Culture No. B67465, in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the said enhanced trait of agronomic importance is conferred under normal watering conditions.
[00487] [00487] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte organism, in which the plant element is a seed.
[00488] [00488] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, in which the endophyte organism is a member of the genus Periconia,
[00489] [00489] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under Deposit NRRL Culture No. 67466, in which the synthetic composition is able to provide an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the plant element is a seed.
[00490] [00490] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the plant element is a seed.
[00491] [00491] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67467 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the plant element is a seed.
[00492] [00492] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under Deposit NRRL Culture No. 67467, where the synthetic composition is able to provide an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the plant element is a seed.
[00493] [00493] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophytic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleot sequence at least 97 % identical to SEQ ID NO: 39, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophyte organism, where the plant element is a seed.
[00494] [00494] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot hint at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the plant element is a seed.
[00495] [00495] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiops and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the plant element is a seed.
[00496] [00496] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleotide sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the plant element is a seed.
[00497] [00497] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter, as deposited under NRRL Culture Deposit No. B67465 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the plant element is a seed.
[00498] [00498] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under Deposit and NRRL culture No. B67465, in which the synthetic composition is capable of providing an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the plant element is a seed.
[00499] [00499] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte organism, in which the plant element is a seed.
[00500] [00500] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, in which the endophyte organism is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the plant element is a seed, in which the seed is modified.
[00501] [00501] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under Deposit NRRL Culture No. 67466, in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the plant element is a seed, in which the seed is modified.
[00502] [00502] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the plant element is a seed, in which the seed is modified.
[00503] [00503] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467 , in which the synthetic composition is capable of providing an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the plant element is a seed, in which the seed is modified.
[00504] [00504] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under Deposit NRRL Culture No. 67467, in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the plant element is a seed, in that the seed is modified.
[00505] [00505] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophytic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleot sequence at least 97 % identical to SEQ ID NO: 39, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophyte organism, where the plant element is a seed, where the seed is modified.
[00506] [00506] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the plant element is a seed, in which the seed is modified.
[00507] [00507] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiops and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the plant element is a seed, in which the seed is modified.
[00508] [00508] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the plant element is a seed, in which the seed is modified.
[00509] [00509] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter, as deposited under NRRL Culture Deposit No. B67465 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the plant element is a seed, in which the seed is modified.
[00510] [00510] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under Deposit and NRRL Culture No. B67465, in which the synthetic composition is capable of providing an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the plant element is a seed, in which the seed is modified.
[00511] [00511] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and
[00512] [00512] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth.
[00513] [00513] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under Deposit of NRRL Culture No. 67466, in which the synthetic composition is able to provide an enhanced trace of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth.
[00514] [00514] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotide sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth.
[00515] [00515] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth.
[00516] [00516] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under Deposit of NRRL Culture No. 67467, in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth.
[00517] [00517] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleot sequence at least 97 % identical to SEQ ID NO: 39, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophyte organism, in which the synthetic composition is placed in a substrate that promotes plant growth.
[00518] [00518] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth.
[00519] [00519] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth.
[00520] [00520] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth.
[00521] [00521] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter, as deposited under NRRL Culture Deposit No. B67465 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth.
[00522] [00522] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged heterologously, wherein the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under Deposit and NRRL Culture No. B67465, in which the synthetic composition is able to provide an enhanced trace of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth.
[00523] [00523] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte organism, where the synthetic composition is placed on a substrate that promotes plant growth, where the substrate that promotes plant growth is soil.
[00524] [00524] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466 , in which the synthetic composition is able to provide an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth, where the substrate that promotes plant growth is soil.
[00525] [00525] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under Deposit of NRRL Culture No. 67466, in which the synthetic composition is able to provide an enhanced trace of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth, where the substrate that promotes plant growth is soil.
[00526] [00526] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophobic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth, where the substrate that promotes plant growth is soil.
[00527] [00527] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467 , in which the synthetic composition is able to provide an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth, where the substrate that promotes plant growth is soil.
[00528] [00528] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under Deposit of NRRL Culture No. 67467, in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth, where the substrate that promotes plant growth is soil.
[00529] [00529] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophobic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleot sequence at least 97 % identical to SEQ ID NO: 39, in which the synthetic composition is able to provide an improved trace of agronomic importance compared to the reference plant element that does not additionally comprise the endophyte organism, in which the synthetic composition is placed in a substrate that promotes plant growth, where the substrate that promotes plant growth is soil.
[00530] [00530] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, in which the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth, where the substrate that promotes plant growth is soil.
[00531] [00531] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth, where the substrate that promotes plant growth is soil.
[00532] [00532] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the Enterobacter genus and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth, where the substrate that promotes plant growth is soil.
[00533] [00533] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophobic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter, as deposited under NRRL Culture Deposit No. B67465 , in which the synthetic composition is able to provide an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth, where the substrate that promotes plant growth is soil.
[00534] [00534] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under Deposit and NRRL Culture No. B67465, in which the synthetic composition is able to provide an enhanced trace of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth, where the substrate that promotes plant growth is soil.
[00535] [00535] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte organism, where the synthetic composition is placed on a substrate that promotes plant growth, where the substrate that promotes plant growth is soil, in which a plurality of said plant elements are placed in the soil in rows, with substantially equal spacing between each seed within each row.
[00536] [00536] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466 , in which the synthetic composition is able to provide an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth, wherein the substrate that promotes plant growth is soil, in which a plurality of said plant elements are placed in the soil in rows, with substantially equal spacing between each seed within each row.
[00537] [00537] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, in which the endophyte organism is an endophyte organism of the genus Periconia derived from the endophyte organism deposited under the Culture Deposit of NRRL No. 67466, in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the synthetic composition is placed on a substrate that promotes the plant growth, in which the substrate that promotes plant growth is soil, in which a plurality of said plant elements are placed in the soil in rows, with substantially equal spacing between each seed within each row.
[00538] [00538] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, where the synthetic composition is placed on a substrate that promotes plant growth, where the substrate that promotes plant growth is soil, in which a plurality of said plant elements are placed in the soil in rows, with substantially equal spacing between each seed within each row.
[00539] [00539] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth, wherein the substrate that promotes plant growth is soil, where a plurality of said plant elements are placed in the soil in rows, with substantially equal spacing between each seed within each row.
[00540] [00540] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is an endophyte organism of the genus Curvularia derived from the endophyte organism deposited under the Culture Deposit of NRRL No. 67467, in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the synthetic composition is placed on a substrate that promotes the plant growth, in which the substrate that promotes plant growth is soil, in which a plurality of said plant elements are placed in the soil in rows, with substantially equal spacing between each seed within each row.
[00541] [00541] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleot sequence at least 97 % identical to SEQ ID NO: 39, in which the synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophyte organism, in which the synthetic composition is placed in a substrate that promotes plant growth, where the substrate that promotes plant growth is soil, where a plurality of said plant elements are placed in the soil in rows, with substantially equal spacing between each seed within each row.
[00542] [00542] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophytic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, where the synthetic composition is placed on a substrate that promotes plant growth, where the substrate that promotes plant growth is soil, in which a plurality of said plant elements are placed in the soil in rows, with substantially equal spacing between each seed within each row.
[00543] [00543] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, where the synthetic composition is placed on a substrate that promotes plant growth, where the substrate that promotes plant growth is soil, in which a plurality of said plant elements are placed in the soil in rows, with substantially equal spacing between each seed within each row.
[00544] [00544] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the Enterobacter genus and comprises at least one polynucleotide sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, where the synthetic composition is placed on a substrate that promotes plant growth, where the substrate that promotes plant growth is soil, in which a plurality of said plant elements are placed in the soil in rows, with substantially equal spacing between each seed within each row.
[00545] [00545] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter, as deposited under NRRL Culture Deposit No. B67465 , in which the synthetic composition is able to provide an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the synthetic composition is placed on a substrate that promotes plant growth, wherein the substrate that promotes plant growth is soil, where a plurality of said plant elements are placed in the soil in rows, with substantially equal spacing between each seed within each row.
[00546] [00546] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under Deposit and NRRL Culture No. B67465, in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the synthetic composition is placed on a substrate which promotes plant growth, where the substrate that promotes plant growth is soil, where a plurality of said plant elements are placed in the soil in rows, with substantially equal spacing between each seed within each row.
[00547] [00547] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that it does not further understand the endophyte organism, the synthetic composition further comprising a formulation comprising one or more of the following: stabilizer, preservative, carrier, surfactant, anti-complex agent or any combination thereof.
[00548] [00548] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466 , in which the synthetic composition is capable of providing an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, the synthetic composition further comprising a formulation comprising one or more of the following: stabilizer, preservative, carrier, surfactant, anti-complex agent or any combination of these.
[00549] [00549] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under Deposit NRRL Culture No. 67466, in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophytic organism, the synthetic composition further comprising a formulation comprising a or more of the following: stabilizer, preservative, carrier, surfactant, anti-complex agent or any combination thereof.
[00550] [00550] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, the synthetic composition further comprising a formulation comprising one or more of the following: stabilizer, preservative, carrier, surfactant, anti-complex agent or any combination thereof.
[00551] [00551] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467 , in which the synthetic composition is capable of providing an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, the synthetic composition further comprising a formulation comprising one or more of the following: stabilizer, preservative, carrier, surfactant, anti-complex agent or any combination of these.
[00552] [00552] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under Deposit NRRL Culture No. 67467, in which the synthetic composition is able to provide an enhanced trace of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, the synthetic composition further comprising a formulation comprising one or more of the following: stabilizer, preservative, carrier, surfactant, anti-complex agent or any combination of these.
[00553] [00553] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleot sequence at least 97 % identical to SEQ ID NO: 39, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophyte organism, the synthetic composition further comprising a formulation that comprises one or more of the following: stabilizer, preservative, carrier, surfactant, anti-complex agent or any combination thereof.
[00554] [00554] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, the synthetic composition further comprising a formulation comprising one or more of the following: stabilizer, preservative, carrier, surfactant, anti-complex agent or any combination thereof.
[00555] [00555] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, the synthetic composition further comprising a formulation comprising one or more of the following: stabilizer, preservative, carrier, surfactant, anti-complex agent or any combination thereof.
[00556] [00556] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, the synthetic composition further comprising a formulation comprising one or more of the following: stabilizer, preservative, carrier, surfactant, anti-complex agent or any combination thereof.
[00557] [00557] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter, as deposited under NRRL Culture Deposit No. B67465 , in which said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophyte organism, the synthetic composition further comprising a formulation comprising one or more of the following: stabilizer , preservative, carrier, surfactant, anti-complex agent or any combination thereof.
[00558] [00558] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under Deposit and NRRL Culture No. B67465, in which the synthetic composition is able to provide an enhanced trace of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, the synthetic composition further comprising a formulation comprising one or more of the following: stabilizer, preservative, carrier, surfactant, anti-complex agent or any combination of these.
[00559] [00559] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte organism, the synthetic composition further comprising a formulation comprising one or more of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
[00560] [00560] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466 , in which the synthetic composition is capable of providing an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, the synthetic composition further comprising a formulation comprising one or more of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
[00561] [00561] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under Deposit NRRL Culture No. 67466, in which the synthetic composition is capable of providing an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, the synthetic composition further comprising a formulation comprising a or more of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
[00562] [00562] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, the synthetic composition further comprising a formulation comprising one or more of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
[00563] [00563] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467 , in which the synthetic composition is capable of providing an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, the synthetic composition further comprising a formulation comprising one or more of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
[00564] [00564] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under Deposit NRRL Culture No. 67467, in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophytic organism, the synthetic composition further comprising a formulation comprising a or more of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
[00565] [00565] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleot sequence at least 97 % identical to SEQ ID NO: 39, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophyte organism, the synthetic composition further comprising a formulation that comprises one or more of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
[00566] [00566] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, the synthetic composition further comprising a formulation comprising one or more of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
[00567] [00567] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophytic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, the synthetic composition further comprising a formulation comprising one or more of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
[00568] [00568] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the Enterobacter genus and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, the synthetic composition further comprising a formulation comprising one or more of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
[00569] [00569] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter, as deposited under NRRL Culture Deposit No. B67465 , in which said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophyte organism, the synthetic composition further comprising a formulation comprising one or more of the following: fungicide , nematicide, bactericide, insecticide or herbicide.
[00570] [00570] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under Deposit and NRRL Culture No. B67465, in which the said synthetic composition is capable of providing an improved trait of agronomic importance in comparison with the reference plant element that does not additionally comprise the modified endophytic organism, the synthetic composition further comprising a formulation comprising a or more of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
[00571] [00571] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte organism, in which said compositions are confined to an object selected from the group consisting of: bottle, flask, ampoule, package, container, bag, box, can, envelope, cardboard box, container, silo, shipping container, bodywork or crate.
[00572] [00572] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the said compositions are confined to an object selected from the group consisting of: bottle, flask, ampoule, package, container, bag, box, can, envelope, cardboard box, container, silo, shipping container, body floor or crate.
[00573] [00573] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under Deposit of NRRL Culture No. 67466, in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which said compositions are confined to an object selected from the group consisting of: bottle, flask, ampoule, package, container, bag, box, can, envelope, cardboard box, container, silo, shipping container, body floor or crate.
[00574] [00574] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which said compositions are confined to an object selected from the group consisting of: bottle, flask, ampoule, package, container, bag, box, can, envelope, cardboard box, container, silo, shipping container, bodywork or crate.
[00575] [00575] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467 , in which the synthetic composition is able to provide an enhanced trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the said compositions are confined to an object selected from the group consisting of: bottle, flask, ampoule, package, container, bag, box, can, envelope, cardboard box, container, silo, shipping container, body floor or crate.
[00576] [00576] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under Deposit NRRL Culture No. 67467, in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which said compositions are confined to an object selected from the group consisting of: bottle, flask, ampoule, package, container, bag, box, can, envelope, cardboard box, container, silo, shipping container, body floor or crate.
[00577] [00577] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleot sequence at least 97 % identical to SEQ ID NO: 39, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophyte organism, in which the said compositions are confined in an object selected from the group consisting of: bottle, flask, ampoule, package, container, bag, box, can, envelope, cardboard box, container, silo, shipping container, body floor or crate.
[00578] [00578] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which said compositions are confined to an object selected from the group consisting of: bottle, flask, ampoule, package, container, bag, box, can, envelope, cardboard box, container, silo, shipping container, bodywork or crate.
[00579] [00579] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that it does not further understand the endophyte organism, in which said compositions are confined to an object selected from the group consisting of: bottle, flask, ampoule, package, container, bag, box, can, envelope, cardboard box, container, silo, transport container , body floor or crate.
[00580] [00580] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the Enterobacter genus and comprises at least one polynucleotide sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which said compositions are confined to an object selected from the group consisting of: bottle, flask, ampoule, package, container, bag, box, can, envelope, cardboard box, container, silo, shipping container, bodywork or crate.
[00581] [00581] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter, as deposited under NRRL Culture Deposit No. B67465 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the said compositions are confined to an object selected from the group consisting of: bottle, flask, ampoule, package, container, bag, box, can, envelope, cardboard box, container, silo, shipping container, body floor or crate.
[00582] [00582] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under Deposit and NRRL culture No. B67465, in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which said compositions are confined to a selected object of the group consisting of: bottle, flask, ampoule, package, container, bag, box, can, envelope, cardboard box, container, silo, shipping container, body floor or crate.
[00583] [00583] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte organism, in which synthetic combinations are stable at room temperature.
[00584] [00584] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, in which the endophyte organism is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the synthetic combinations are stable at room temperature.
[00585] [00585] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under Deposit NRRL Culture No. 67466, in which the synthetic composition is able to provide an enhanced trace of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the synthetic combinations are stable at room temperature .
[00586] [00586] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which synthetic combinations are stable at room temperature.
[00587] [00587] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the synthetic combinations are stable at room temperature.
[00588] [00588] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under Deposit NRRL Culture No. 67467, in which the synthetic composition is able to provide an enhanced trace of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the synthetic combinations are stable at room temperature .
[00589] [00589] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleot sequence at least 97 % identical to SEQ ID NO: 39, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophytic organism, in which the synthetic combinations are stable at room temperature.
[00590] [00590] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which synthetic combinations are stable at room temperature.
[00591] [00591] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiops and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which synthetic combinations are stable at room temperature.
[00592] [00592] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49-
[00593] [00593] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the Enterobacter genus, as deposited under NRRL Culture Deposit No. B67465 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism, in which the synthetic combinations are stable at room temperature.
[00594] [00594] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under Deposit and NRRL Culture No. B67465, in which the synthetic composition is able to provide an enhanced trace of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism, in which the synthetic combinations are stable at room temperature .
[00595] [00595] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte, in which the identity percentage is at least 98%.
[00596] [00596] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to each of the sequences in the group consisting of SEQ ID NO: 32 and 33, where the percentage of identity is at least 98%.
[00597] [00597] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the percentage of identity is at least 98%.
[00598] [00598] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 34-38, where the percentage of identity is at least 98%.
[00599] [00599] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least three sequences selected from the group consisting of SEQ ID NOs: 34-38, where the percentage of identity is at least 98%.
[00600] [00600] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least four sequences selected from the group consisting of SEQ ID NOs: 34-38, where the percentage of identity is at least 98%.
[00601] [00601] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotide sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least five sequences selected from the group consisting of SEQ ID NOs: 34-38, where the percentage of identity is at least 98%.
[00602] [00602] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleot sequence at least 97 % identical to SEQ ID NO: 39, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophyte organism, in which the percentage of identity is at least minus 98%.
[00603] [00603] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophobic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the percentage of identity is at least 98%.
[00604] [00604] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 40-43, where the percentage of identity is at least 98%.
[00605] [00605] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophytic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-
[00606] [00606] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least four sequences selected from the group consisting of SEQ ID NOs: 40-43, where the percentage of identity is at least 98%.
[00607] [00607] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, in which the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 44-48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that it does not understand additionally the endophyte organism, in which the percentage of identity is at least 98%.
[00608] [00608] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44-
[00609] [00609] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophytic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least three sequences selected from the group consisting of SEQ ID NOs: 44-48, where the percentage of identity is at least 98%.
[00610] [00610] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, where the endophyte organism comprises polynucleotide sequences at least 97% identical to at least four sequences selected from the group consisting of SEQ ID NOs: 44-48, where the percentage of identity is at least 98%.
[00611] [00611] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophytic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least five sequences selected from the group consisting of SEQ ID NOs: 44-48, where the percentage of identity is at least 98%.
[00612] [00612] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97 % identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that it does not understand additionally the endophytic organism, in which the percentage of identity is at least 98%.
[00613] [00613] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 49-51, where the percentage of identity is at least 98%.
[00614] [00614] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophobic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least three sequences selected from the group consisting of SEQ ID NOs: 49-51, where the percentage of identity is at least 98%.
[00615] [00615] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte organism, in which the percentage of identity is at least 99%.
[00616] [00616] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to each of the sequences in the group consisting of SEQ ID NO: 32 and 33, where the percentage of identity is at least 99%.
[00617] [00617] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the percentage of identity is at least 99%.
[00618] [00618] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 34-38, where the percentage of identity is at least 99% .
[00619] [00619] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 34-38, where the percentage of identity is at least 99%.
[00620] [00620] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least four sequences selected from the group consisting of SEQ ID NOs: 34-38, where the percentage of identity is at least 99%.
[00621] [00621] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least five sequences selected from the group consisting of SEQ ID NOs: 34-38, where the percentage of identity is at least 99%.
[00622] [00622] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleot sequence at least 97 % identical to SEQ ID NO: 39, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophyte organism, in which the identity percentage is at minus 99%.
[00623] [00623] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that it does not further understand the endophyte organism, in which the percentage of identity is at least 99%.
[00624] [00624] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 40-43, where the percentage of identity is at least 99%.
[00625] [00625] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophytic organism, in which the endophytic organism comprises polynucleotide sequences at least 97% identical to at least three sequences selected from the group consisting of SEQ ID NOs: 40-43, where the percentage of identity is at least 99%.
[00626] [00626] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least four sequences selected from the group consisting of SEQ ID NOs: 40-43, where the percentage of identity is at least 99%.
[00627] [00627] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 44-48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that it does not understand additionally the endophyte organism, in which the percentage of identity is at least 99%.
[00628] [00628] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 44-48, where the percentage of identity is at least 99%.
[00629] [00629] In one aspect, the invention provides a synthetic composition that comprises a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least three sequences selected from the group consisting of SEQ ID NOs: 44-48, where the percentage of identity is at least 99%.
[00630] [00630] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least four sequences selected from the group consisting of SEQ ID NOs: 44-48, where the percentage of identity is at least 99%.
[00631] [00631] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least five sequences selected from the group consisting of SEQ ID NOs: 44-48, where the percentage of identity is at least 99%.
[00632] [00632] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97 % identical to a polynucleot sequence is selected from the group consisting of SEQ ID NOs: 49-51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that it does not understand additionally the endophyte organism, in which the percentage of identity is at least 99%.
[00633] [00633] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the Enterobacter genus and comprises at least one polynucleotide sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 49-51, where the percentage of identity is at least 99%.
[00634] [00634] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophobic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 49-51, where the percentage of identity is at least 99%.
[00635] [00635] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte organism, in which the percentage of identity is 100%.
[00636] [00636] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophobic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to each of the sequences within the group consisting of SEQ ID NO: 32 and 33, where the percentage of identity is 100%.
[00637] [00637] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the percentage of identity is 100%.
[00638] [00638] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-
[00639] [00639] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 34-38, where the percentage of identity is 100%.
[00640] [00640] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophytic organism, in which the endophytic organism comprises polynucleotide sequences at least 97% identical to at least four sequences selected from the group consisting of SEQ ID NOs: 34-38, where the percentage of identity is 100%.
[00641] [00641] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-
[00642] [00642] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleotic sequence at least 97 % identical to SEQ ID NO: 39, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophyte organism, in which the percentage of identity is at least minus 100%.
[00643] [00643] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the percentage of identity is at least 100%.
[00644] [00644] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, in which the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least two sequences selected from the group consisting of SEQ ID NO: 40-43, where the percentage of identity is 100%.
[00645] [00645] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophytic organism, in which the endophytic organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 40-43, where the percentage of identity is 100%.
[00646] [00646] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least four sequences selected from the group consisting of SEQ ID NOs: 40-43, where the identity percentage is at least 100%.
[00647] [00647] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 44-48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that it does not understand additionally the endophyte organism, in which the percentage of identity is 100%.
[00648] [00648] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 44-48, where the percentage of identity is at least 100%.
[00649] [00649] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least three sequences selected from the group consisting of SEQ ID NOs: 44-48, where the percentage of identity is 100%.
[00650] [00650] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least four sequences selected from the group consisting of SEQ ID NOs: 44-48, where the percentage of identity is 100%.
[00651] [00651] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophytic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least five sequences selected from the group consisting of SEQ ID NOs: 44-48, where the percentage of identity is 100%.
[00652] [00652] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleotic sequence at least 97% identical to a polynucleot hint sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not it additionally comprises the endophyte organism, in which the percentage of identity is 100%.
[00653] [00653] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 49-51, where the percentage of identity is 100%.
[00654] [00654] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least three sequences selected from the group consisting of SEQ ID NOs: 49-51, where the percentage of identity is 100%.
[00655] [00655] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte organism, in which the percentage of identity is determined over an alignment region of at least 100 nucleotides.
[00656] [00656] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to each of the sequences within the group consisting of SEQ ID NO: 32 and 33, in which the percentage of identity is determined over a region of alignment of at least 100 nucleotides.
[00657] [00657] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the percentage of identity is determined over an alignment region of at least 100 nucleotides.
[00658] [00658] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over a region alignment of at least 100 nucleotides.
[00659] [00659] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over a region alignment of at least 100 nucleotides.
[00660] [00660] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophobic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least four sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over a region alignment of at least 100 nucleotides.
[00661] [00661] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least five sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over a region alignment of at least 100 nucleotides.
[00662] [00662] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleot sequence at least 97 % identical to SEQ ID NO: 39, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophyte organism, in which the identity percentage is determined over an alignment region of at least 100 nucleotides.
[00663] [00663] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that it does not further understand the endophyte organism, in which the percentage of identity is determined over an alignment region of at least 100 nucleotides.
[00664] [00664] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over a region alignment of at least 100 nucleotides.
[00665] [00665] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophilic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over a region alignment of at least 100 nucleotides.
[00666] [00666] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least four sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over a region alignment of at least 100 nucleotides.
[00667] [00667] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the percentage of identity is determined over an alignment region of at least 100 nucleotides.
[00668] [00668] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophytic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over a region alignment of at least 100 nucleotides.
[00669] [00669] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least three sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over a region alignment of at least 100 nucleotides.
[00670] [00670] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least four sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over a region alignment of at least 100 nucleotides.
[00671] [00671] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least five sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over a region alignment of at least 100 nucleotides.
[00672] [00672] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the Enterobacter genus and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the percentage of identity is determined over an alignment region of at least 100 nucleotides.
[00673] [00673] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 49-51, in which the percentage of identity is determined over a region alignment of at least 100 nucleotides.
[00674] [00674] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least three sequences selected from the group consisting of SEQ ID NOs: 49-51, in which the percentage of identity is determined throughout the region of 100 nucleotide alignment.
[00675] [00675] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte organism, in which the percentage of identity is determined over an alignment region of at least 200 nucleotides.
[00676] [00676] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and
[00677] [00677] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the percentage of identity is determined over an alignment region of at least 200 nucleotides.
[00678] [00678] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over a region alignment of at least 200 nucleotides.
[00679] [00679] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over a region alignment of at least 200 nucleotides.
[00680] [00680] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least four sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over a region alignment of at least 200 nucleotides.
[00681] [00681] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least five sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined across a region alignment of at least 200 nucleotides.
[00682] [00682] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism disposed in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleot sequence at least 97 % identical to SEQ ID NO: 39, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophytic organism, in which the identity percentage is determined over an alignment region of at least 200 nucleotides.
[00683] [00683] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the percentage of identity is determined over an alignment region of at least 200 nucleotides.
[00684] [00684] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, in which the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over a region alignment of at least 200 nucleotides.
[00685] [00685] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least three sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over a region alignment of at least 200 nucleotides.
[00686] [00686] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least four sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over a region alignment of at least 200 nucleotides.
[00687] [00687] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44-
[00688] [00688] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over a region alignment of at least 200 nucleotides.
[00689] [00689] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over a region alignment of at least 200 nucleotides.
[00690] [00690] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least four sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over a region alignment of at least 200 nucleotides.
[00691] [00691] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophytic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least five sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over a region alignment of at least 200 nucleotides.
[00692] [00692] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the percentage of identity is determined over an alignment region of at least 200 nucleotides.
[00693] [00693] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 49-51, in which the percentage of identity is determined over a region alignment of at least 200 nucleotides.
[00694] [00694] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the Enterobacter genus and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 49-51, in which the percentage of identity is determined over a region alignment of at least 200 nucleotides.
[00695] [00695] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte organism, in which the percentage of identity is determined over an alignment region of at least 300 nucleotides.
[00696] [00696] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to each of the sequences in the group consisting of SEQ ID NO: 32 and 33, in which the percentage of identity is determined along an alignment region of at least 300 nucleotides.
[00697] [00697] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the percentage of identity is determined over an alignment region of at least 300 nucleotides.
[00698] [00698] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over a region alignment of at least 300 nucleotides.
[00699] [00699] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over a region alignment of at least 300 nucleotides.
[00700] [00700] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least four sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over a region alignment of at least 300 nucleotides.
[00701] [00701] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least five sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over a region alignment of at least 300 nucleotides.
[00702] [00702] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleot sequence at least 97 % identical to SEQ ID NO: 39, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophyte organism, in which the identity percentage is determined over an alignment region of at least 300 nucleotides.
[00703] [00703] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the percentage of identity is determined over an alignment region of at least 300 nucleotides.
[00704] [00704] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophobic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over a region alignment of at least 300 nucleotides.
[00705] [00705] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over a region alignment of at least 300 nucleotides.
[00706] [00706] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophobic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises hint polynucleot sequences at least
[00707] [00707] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that it does not further understand the endophyte organism, in which the percentage of identity is determined over an alignment region of at least 300 nucleotides.
[00708] [00708] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over a region alignment of at least 300 nucleotides.
[00709] [00709] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over a region alignment of at least 300 nucleotides.
[00710] [00710] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, in which the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least four sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over a region alignment of at least 300 nucleotides.
[00711] [00711] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophytic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least five sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over a region alignment of at least 300 nucleotides.
[00712] [00712] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the percentage of identity is determined over an alignment region of at least 300 nucleotides.
[00713] [00713] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the Enterobacter genus and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 49-51, in which the percentage of identity is determined over a region alignment of at least 300 nucleotides.
[00714] [00714] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 49-51, in which the percentage of identity is determined over a region alignment of at least 300 nucleotides.
[00715] [00715] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophobic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte organism, in which the percentage of identity is determined over an alignment region of at least 400 nucleotides.
[00716] [00716] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to each of the sequences in the group consisting of SEQ ID NO: 32 and 33, in which the percentage of identity is determined along an alignment region of at least 400 nucleotides.
[00717] [00717] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the percentage of identity is determined over an alignment region of at least 400 nucleotides.
[00718] [00718] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over a region alignment of at least 400 nucleotides.
[00719] [00719] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over a region alignment of at least 400 nucleotides.
[00720] [00720] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-
[00721] [00721] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least five sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over a region alignment of at least 400 nucleotides.
[00722] [00722] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleot sequence at least 97 % identical to SEQ ID NO: 39, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophyte organism, in which the identity percentage is determined over an alignment region of at least 400 nucleotides.
[00723] [00723] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, in which the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the percentage of identity is determined over an alignment region of at least 400 nucleotides.
[00724] [00724] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over a region alignment of at least 400 nucleotides.
[00725] [00725] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophytic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over a region alignment of at least 400 nucleotides.
[00726] [00726] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least four sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over a region alignment of at least 400 nucleotides.
[00727] [00727] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the percentage of identity is determined over an alignment region of at least 400 nucleotides.
[00728] [00728] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined across a region alignment of at least 400 nucleotides.
[00729] [00729] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least three sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over a region alignment of at least 400 nucleotides.
[00730] [00730] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least four sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over a region alignment of at least 400 nucleotides.
[00731] [00731] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least five sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over a region alignment of at least 400 nucleotides.
[00732] [00732] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte, in which the percentage of identity is to be determined over an alignment region of at least 400 nucleotides.
[00733] [00733] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 49-51, in which the percentage of identity is determined over a region alignment of at least 400 nucleotides.
[00734] [00734] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 49-51, in which the percentage of identity is determined over a region alignment of at least 400 nucleotides.
[00735] [00735] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally understand the endophyte organism, in which the percentage of identity is determined over at least one alignment region of at least 500 nucleotides.
[00736] [00736] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Periconia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NO: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to each of the sequences in the group consisting of SEQ ID NO: 32 and 33, in which the percentage of identity is determined along an alignment region of at least 500 nucleotides.
[00737] [00737] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophobic organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the percentage of identity is determined over an alignment region of at least 500 nucleotides.
[00738] [00738] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over a region alignment of at least 500 nucleotides.
[00739] [00739] In one aspect, the invention provides a synthetic composition comprising a plant element and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises hint polynucleot sequences at least
[00740] [00740] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least four sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over at least an alignment region of at least 500 nucleotides.
[00741] [00741] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophytic organism, in which the endophytic organism comprises polynucleotide sequences at least 97% identical to at least five sequences selected from the group consisting of SEQ ID NOs: 34-38, in which the percentage of identity is determined over a region of 500 nucleotide alignment.
[00742] [00742] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleot sequence at least 97 % identical to SEQ ID NO: 39, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophytic organism, in which the identity percentage is determined over an alignment region of at least 500 nucleotides.
[00743] [00743] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte, in which the percentage of identity is to be determined over an alignment region of at least 500 nucleotides.
[00744] [00744] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over at least 500 nucleotides.
[00745] [00745] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least three sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over a region alignment of at least 500 nucleotides.
[00746] [00746] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 40- 43, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least four sequences selected from the group consisting of SEQ ID NOs: 40-43, in which the percentage of identity is determined over a alignment region of at least 500 nucleotides.
[00747] [00747] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte, in which the percentage of identity is to be determined over an alignment region of at least 500 nucleotides.
[00748] [00748] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least two sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over a region alignment of at least 500 nucleotides.
[00749] [00749] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least three sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over a region alignment of at least 500 nucleotides.
[00750] [00750] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least four sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over at least 500 nucleotides.
[00751] [00751] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleot sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 44- 48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least five sequences selected from the group consisting of SEQ ID NOs: 44-48, in which the percentage of identity is determined over a region alignment of at least 500 nucleotides.
[00752] [00752] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the Enterobacter genus and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism, in which the percentage of identity is determined over an alignment region of at least 500 nucleotides.
[00753] [00753] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleotide sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises polynucleotide sequences at least 97% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 49-51, in which the percentage of identity is determined over a region alignment of at least 500 nucleotides.
[00754] [00754] In one aspect, the invention provides a synthetic composition comprising an element of the plant and an endophyte organism arranged in a heterologous manner, wherein the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleotic sequence at least 97 % identical to a sequence selected from the group consisting of SEQ ID NOs: 49- 51, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally understand the endophyte organism, in which the endophyte organism comprises at least 97% polynucleotide sequences identical to at least three sequences selected from the group consisting of SEQ ID NOs: 49-51, in which the percentage of identity is determined over a region alignment of at least 500 nucleotides. BRIEF DESCRIPTION OF THE FIGURES
[00755] [00755] Figure 1: Root length (cm) of soybean seedlings as a percentage of the average root length of control soybean seedlings not treated with a microbe. The seedlings were grown as described in Example
[00756] [00756] Figure 2: Root surface area (cm2) of soybean seedlings as a percentage of the average root surface area of soybean seedlings not treated with microbes. The seedlings were grown as described in Example 4. DETAILED DESCRIPTION
[00757] [00757] As demonstrated in this document, agricultural plants are heterologously arranged in symbiotic microorganisms, called endophyte organisms, particularly bacteria and fungi, which contribute to the survival, performance and characteristics of plants.
[00758] [00758] This document describes endophyte organisms that are capable of living inside or otherwise arranged heterologously in plants to improve the characteristics of the plant. Methods of using endophyte organisms that are heterologously arranged in plants to impart new characteristics to a host plant, as well as distinguishing plant elements from the host plant, are described in this document. In some embodiments, the endophytic compositions are isolated and purified from sources of plants or fungi and arranged heterologously with an element of the plant to transmit improved agronomic potential and / or improved agronomic traits to the host plant. In some modalities, the endophyte organisms that are able to live inside the plants are isolated and purified from their native sources and arranged in a heterologous way, for example, manually, mechanically or artificially combined, with a plant element, to confer a better agronomic potential and / or improved agronomic traits for the host plant or elements of the host plant. Such endophytic organisms that are capable of living within plants can be further manipulated or combined with additional elements before being combined with the elements of the plant.
[00759] [00759] As described in this document, endophyte organisms can be robustly derived from heterologous, homologous or manipulated sources, optionally - cultivated, applied manually, mechanically or artificially applied heterologously to plant elements, for example, heterologously arranged and, as a result of manual, mechanical or artificial application, they confer various beneficial properties. This is surprising, given the variability observed in the art in isolating endophytic microbes and previous observations of inefficient colonization of plant pathogens from host plant tissues.
[00760] [00760] In part, the present invention provides preparations of endophyte organisms that are capable of living within plants, and the creation of synthetic compositions of plant elements and / or seedlings with heterologously arranged endophyte organisms, and formulations comprising the compositions synthetic, as well as the recognition that synthetic compositions have a diversity of beneficial and unexpected properties present in agricultural plants and / or in populations of heterologous endophyte organisms. Beneficial properties include, but are not limited to, metabolism, transcript expression, proteomic changes, morphology, resilience to a variety of environmental stresses and any combination of such properties. The present invention also provides methods of using endophyte organisms described in this document to benefit the host plant with which they are heterologously arranged.
[00761] [00761] The terms used in the claims and specifications are defined as set out below, unless otherwise specified.
[00762] [00762] It should be noted that, as used in this specification and in the appended claims, the singular forms "a" "a" and "o / a" include plural referents, unless the context clearly dictates otherwise.
[00763] [00763] An "endophyte organism" is an organism capable of living in a plant element (for example, rhizoplane or phyllosphere) or within a plant element, or on a close physical surface of a plant element, for example example, the rhizosphere, or in a seed. A "beneficial" endophyte organism does not cause disease or harm the host plant. Endophyte organisms can occupy the intracellular or extracellular spaces of plant tissue, including leaves, stems, flowers, fruits, seeds or branches. An endophyte organism can be, for example, a bacterial or fungal organism and can confer a beneficial property on the host plant, such as an increase in yield, biomass, resistance or fitness. An endophyte organism can be a fungus or a bacterium. As used in this document, the term "microbe" is sometimes used to describe an endemic organism.
[00764] [00764] A "population" of endophyte organisms, or an "endophyte population", refers to one or more endophyte organisms that share a common genetic derivation, for example, one or more propagules of a single endophyte organism, that is, endophyte organisms created from a single chosen colony. In some modalities, a population refers to endophyte organisms of identical taxonomy. In some cases, a population of endophyte organisms refers to one or more endophyte organisms of the same genus. In some cases, a population of endophyte organisms refers to one or more endophyte organisms from the same OTU.
[00765] [00765] A "plurality of endophyte organisms" means two or more types of endophytic entities, for example, bacteria or fungi, or combinations thereof. In some embodiments, the two or more types of endophytic entities are two or more individual endophytic organisms, regardless of genetic derivation or taxonomic relationship. In some embodiments, the two or more types of endophytic entities are two or more populations of endophyte organisms. In other embodiments, the two or more types of endophytic entities are two or more species of endophytic organisms. In still other modalities, the two or more types of endophyte entities are two or more genera of endophyte organisms. In still other embodiments, the two or more types of endophyte entities are two or more families of endophyte organisms. In other embodiments, the two or more types of endophytic entities are two or more orders of endophyte organisms. In other embodiments, the two or more types of endophytic entities are two or more classes of endophyte organisms. In still other modalities, the two or more types of endophyte entities are two or more phyla of endophyte organisms. In some embodiments, a plurality refers to three or more endúfitos organisms, distinct individual organisms or distinct members of different genetic derivation or taxa. In some embodiments, a plurality refers to four or more distinct individual endophytic organisms or distinct members of different genetic derivations or taxa. In some embodiments, a plurality refers to five or more, ten or more, or an even greater number of distinct individual endophytic organisms or distinct members of different genetic derivations or taxa. In some modalities, the term "consortium" or "consortium" can be used as a collective noun synonymous with "plurality", when describing more than one population, species, genus, family, order, class or phylum of endophyte organisms.
[00766] [00766] As "used in this document, the term" microbe "or" microorganism "refers to any species or taxon of microorganism, including, but not limited to, archaea, bacteria, microalgae, fungi (including mold and yeast species) ), mycoplasmas, microspores, nanobacteria, oomycetes and protozoa. In some embodiments, a microbe or microorganism is an endophyte organism, for example, a bacterial or fungal endophyte organism, which is capable of living inside a plant. In some embodiments, a microbe or microorganism comprises individual cells (eg, single-celled microorganisms) or more than one cell (eg, multicellular microorganism). A "population of microorganisms" can therefore refer to multiple cells of a single microorganism, in which the cells share a common genetic derivation.
[00767] [00767] As used in this document, the term "bacterium" or "bacterium" refers in general to any prokaryotic organism, and can refer to an organism from Kingdom Eubacteria (Bacteria), Kingdom Archeobacteria (Archae), or both. In some cases, bacterial genera have been reassigned for various reasons (such as, but not limited to, the evolving field of complete genome sequencing), and it is understood that such nomenclature reassignments are within the scope of any claimed genus . For example, certain species of the genus Enwinia have been described in the literature as belonging to the genus Pantoea (Zhang, Y. & Qiu, S. Antonie van Leeuwenhoek (2015) 108: 1037).
[00768] [00768] The term 168 refers to the DNA sequence of the ribosomal RNA sequence 168 (r »RNA) of a bacterium. Sequencing the 168 rRNA gene is a well-established method for studying the phylogeny and taxonomy of bacteria.
[00769] [00769] As used in this document, the term "fungus" or "fungus" refers in general to any organism in the Fungi Kingdom. The historical taxonomic classification of fungi is in accordance with the morphological presentation. From the mid-1800s onwards, it was recognized that some fungi have a pleomóárfico life cycle and that different nomenclature designations were being used for different forms of the same fungus. In 1981, the Sydney Congress of the International Mycological Association established rules for naming fungi according to their status as anamorph, teleomorph or holomorph (Taylor JW. One Fungus = One Name: DNA and fungal nomenciature twenty years after PCR. IMA Fungus 2 (2): 113-120.2011.). With the development of genomic sequencing, it became evident that the taxonomic classification based on molecular phylogenetics did not align with the morphological nomenclature (Shenoy BD, Jeewon R, Hyde KD. Impact of DNA sequence-data on the taxonomy of anamorphic fungi. Fungal Diversity 26 (10) 1-54. 2007). As a result, in 2011, the International Botanical Congress
[00770] [00770] "Internal Transcribed Spacer" (ITS) refers to the spacer DNA (non-coding DNA) located between the small subunit ribosomal RNA (rRNA) and large subunit rRNA (LSU) genes on the chromosome or the transcribed region corresponding to the region transcribed in the polycistronic rRNA precursor transcript. Sequencing of ITS genes is a well-established method for studying the phylogeny and taxonomy of fungi. In some cases, the "Large SubUnit" (LSU) sequence is used to identify fungi. LSU genetic sequencing is a well-established method for studying phylogeny and taxonomy of fungi. Some endophytic fungal organisms can be described by an ITS sequence and some can be described by an LSU string, both of which are understood to be equally descriptive and accurate in determining taxonomy.
[00771] [00771] As used in this document in relation to fungi and bacteria, the term "marker gene" refers to a conserved gene comprising sequence variation between related organisms, for example, a polynucleot tip 168 (for bacteria) or ITS ( for fungi) of the organism, fusA gene or genomic regions, by which a microbe can be specifically identified and assigned to a taxonomic nomenclature. In some embodiments, marker genes include, but are not limited to, long subunit rRNA (LSU) gene, second largest RNA polymerase subunit | (RPB2), small subunit rRNA (SSU) gene, L 10 60S ribosomal protein, beta-tubulin and combinations thereof.
[00772] [00772] The terms "pathogen" and "pathogenic" in reference to a bacterium or fungus include any organism of that type that is capable of causing or affecting a disease, disorder or condition of a host comprising the organism.
[00773] [00773] A "spore" or a population of "spores" refers to bacteria or fungi that are generally viable, more resistant to environmental influences such as heat and bactericidal or fungicidal agents than other forms of the same bacteria or fungi, and typically capable of germination and growth. Bacteria and fungi that are "capable of forming spores" are those bacteria and fungi that comprise the genes and other skills necessary to produce spores under appropriate environmental conditions.
[00774] [00774] "Biomass" means the total mass or weight (fresh or dry), at a given moment, of a plant tissue, plant tissues, an entire plant or a population of plants. Biomass is generally given as weight per unit area. The term can also refer to all plants or species in the community (community biomass).
[00775] [00775] The term "isolated" is intended to refer specifically to an organism, cell, tissue, polynucleot deo or polypeptide that is removed from its original source.
[00776] [00776] As used herein, an isolated endophyte organism or microbe is an endophyte organism or microbe that has been removed from its natural environment. “Pure cultures” or “isolated cultures” are cultures in which the organisms present are only of a particular genus and species. This contrasts with "mixed cultures", which are cultures in which more than one genus and / or species of microorganism are present. As such, the term "isolated" does not necessarily reflect the extent to which the microbe has been purified. A "substantially pure culture" of the microbe refers to a culture that contains substantially no other endophyte or microbe organism than the desired endophyte or microbe. In other words, a substantially pure endophyte organism or microbial culture is substantially free of other contaminants, which may include microbial contaminants. In addition, as used in this document, "biologically pure" is meant to mean the endophyte or microbe separate from the materials with which it is normally found in nature. An endophyte microbe or organism disposed heterologously to other endophyte microbes or organisms, or with compounds or materials with which it is not normally found in nature, is still defined as "biologically pure". A monoculture is, of course, "biologically pure". As used herein, the term "enriched culture" of an isolated endophyte microbe or organism refers to a culture that contains more than 50%, 60%, 70%, 80%, 90% or 95% of the endophyte or microbe isolated.
[00777] [00777] A "host plant" includes any plant, particularly a plant of agronomic importance, within which or on which a microbe, such as an endophyte organism, is discarded heterologously. As used in this document, a microbe is said to colonize a plant, plant element or seed, when it can exist as an endophyte organism in relation to a plant or plant element during at least part of the life cycle of the plant or microbe. In some embodiments, an endophyte organism is said to "colonize" a plant or plant element when it can be stably detected within the plant or plant element for a period of time,
[00778] [00778] A "non-host target" means an organism or chemical compound that is altered in some way after coming into contact with a host plant that comprises an endophyte organism, as a result of a property conferred on the host plant by the endophyte organism.
[00779] [00779] As used herein, a nucleic acid has "homology" or is "homologous" to a second nucleic acid if the nucleic acid sequence has a sequence similar to the second nucleic acid sequence. The terms "identity", "percent identity", "percent sequence identity" or "identical" in the context of nucleic acid sequences refer to the nucleotides in the two sequences that are the same when aligned for maximum match. There are different algorithms known in the art that can be used to measure nucleotide sequence identity. The identity of the nucleotide sequence can be measured by local or global alignment, preferably implementing an optimal local or optimal global alignment algorithm. For example, a global alignment can be generated using an implementation of the Needleman-Wunsch algorithm (Needleman, SB & Wunsch, C.D (1970) Journal of Molecular Biology. 48 (3): 443-53). For example, a local alignment can be generated using an implementation of the Smith-Waterman algorithm (Smith T.F & Waterman, M.S (1981) Journal of Molecular Biology. 147 (1): 195-197). Optimal global alignments using the Needleman-Wunsch algorithm and optimal local alignments using the Smith-Waterman algorithm are implemented in USEARCH, for example, USEARCH version v8.1.1756 i860sx32.
[00780] [00780] A gap is a region of an alignment in which one sequence does not align with a position in the other alignment sequence. In global alignments, terminal gaps are discarded before identity is calculated. For local and global alignments, internal gaps are counted as differences. A terminal gap is a region that begins at the end of a sequence in an alignment in which the nucleotide deo in the terminal position of that sequence does not correspond to a nucleotile position in the other sequence of the alignment and extends to all contiguous positions in that alignment sequence in which the nucleotides of that sequence do not correspond to a nucleotide position in the other alignment sequence. An internal gap is a gap in an alignment that is flanked at the 3 'and 5' ends by positions where the aligned strings are identical.
[00781] [00781] In some embodiments, the nucleic acid sequence to be aligned is a complete gene. In some embodiments, the nucleic acid sequence to be aligned is a gene fragment. In some embodiments, the nucleic acid sequence to be aligned is an intergenic sequence. In a preferred embodiment, homology inference from a sequence alignment is made where the alignment region is at least 85% of the length of the query sequence.
[00782] [00782] The term "substantial homology" or "substantial similarity", when referring to a nucleic acid or fragment thereof, indicates that, when optimally aligned with appropriate nucleotide insertions or deletions with another nucleic acid (or its complementary strand) , there is nucleotide sequence identity in at least about 76%, 80%, 85%, or at least about 90%, or at least about 95%, 96%, at least 97%, 98%, 99% or 100% of the alignment positions, where the alignment region is at least about 50%, 60%, 70%, 75%, 85% or at least about 90%, or at least about 95%, 96% , 97%, 98%, 99% or 100% of the query string length. In a preferred embodiment, the alignment region contains at least 100 positions, including any internal gaps. In some embodiments, the alignment region comprises at least 100 nucleotiles of the query sequence. In some embodiments, the alignment region comprises at least 200 nucleotides of the query sequence. In some embodiments, the alignment region comprises at least 300 nucleotides of the query sequence. In some embodiments, the alignment region comprises at least 400 nucleotides of the query sequence. In some embodiments, the alignment region comprises at least 500 nucleotides of the query sequence. In some modalities, the query sequence is selected from the group consisting of SEQ ID NOs: 32-51.
[00783] [00783] As used in this document, the terms "operational taxonomic unit", "OTU", "taxon", "hierarchical cluster" and "cluster" are used interchangeably. An operational taxon unit (OTU) refers to a group of one or more organisms that comprises a node in a cluster tree. The level of a grouping is determined by its hierarchical order. In some modalities, an OTU is a group provisionally considered to be a valid taxon for purposes of phylogenetic analysis. In another modality, an OTU is any of the existing taxonomic units under study. In yet another modality, an OTU receives a name and a classification. For example, an OTU may represent a gift, a subdomain, a kingdom, a sub-kingdom, a phylum, a subphile, a class, a subclass, an order, a suborder, a family, a subfamily, a genus, a subgenus or a species. In some modalities, OTUs can represent one or more organisms from the Eubacteria, Bacteria, Protista or Fungi kingdoms, at any level of a hierarchical order. In some modalities, an OTU represents a prokaryotic or fungal order.
[00784] [00784] In some embodiments, the present invention contemplates synthetic compositions comprising the combination of a plant element, seedlings or whole plants and an endophytic population, in which the endophytic population is "discarded heterologously". In some embodiments, one or more endophyte organisms of the synthetic composition are arranged heterologously or manually, artificially inoculated or arranged in a plant element, seedlings, plants or in a plant growth medium or in one or more formulation of treatment so that the endophyte organism exists on or in said plant element, seedling, plant, plant growth medium or treatment formulation in a manner not found in nature before the application of one or more endophyte organisms, for example, the said combination that is not found in nature. In some modalities, such a way is contemplated to be selected from the group consisting of: the presence of the endophyte organism; presence of the endophyte organism in a different number of cells, concentration or quantity; the presence of the endophyte organism in a plant element, tissue, cell type or other different physical location on the plant; the presence of the endophyte organism in different periods of time, for example, stage of development of the plant or plant element, time of day, time of the season and combinations of these. In some modalities,
[00785] [00785] An "inoculated" plant or plant element was artificially introduced into a heterologous endophytic organism at some point during the growth or development of the plant or plant element (including vegetative or reproductive phases). In some embodiments, the heterologous endophyte organism is transiently or permanently incorporated into the plant or plant element and is detectable using methods known in the art or described in this document. In some embodiments, a seed is inoculated with an endophyte organism by manual or mechanical contact with the seed with a formulation comprising said endophyte organism, which is detectable in or on the seed. In some embodiments, a plant is said to be inoculated with an endophyte if it is grown from a reproductive element (for example, a seed) that has been contacted manually or mechanically with a formulation - comprising the said endophytic organism, which is subsequently detectable in the plant. In some embodiments, a plant is said to be inoculated with an endophyte organism if any or more of its plant elements (for example, leaf, stem or root) are contacted manually or mechanically with a formulation comprising said endophyte organism, which is subsequently detectable in the same plant element that was originally contacted with the said formulation or in a different plant element of that plant. The term “inoculation” can also refer to the manual or mechanical contact of an endophytic population with any substance, which is detectable in or on that substance after contact with the endophyte organism. In one example, said substance is soil or another plant growth medium. In another example, said substance is a storage medium such as glycerol. In some cases, "inoculation" may refer to the contact of a population of endophyte organisms with a living non-plant organism, for example, but not limited to, an insect or fungus.
[00786] [00786] The term "isoline" is a comparative term and refers to organisms that are genetically identical, but that may differ in treatment. In one example, two genetically identical corn plant embryos can be separated into two different groups, one receiving a treatment (such as transformation with a heterologous polynucleotide, to create a genetically modified plant) and a control, for example, reference, which does not receive such treatment. Any phenotypic differences between the two groups can therefore be attributed only to the treatment and not to any inheritance of the genetic makeup of the plant. In another example, two genetically identical soybeans can be treated with a formulation, one that introduces an endoffic composition and one that does not. Any phenotypic differences between the plants derived (for example, grown or obtained from) these seeds can be attributed to the treatment with endophyte organisms, thus forming a comparison of insulins.
[00787] [00787] Likewise, the term "reference agricultural plant" means an agricultural plant of the same species, variety or cultivar, for which a treatment, formulation, composition or preparation of an endophytic organism as described in this document is not administered / contacted. A reference agricultural plant, therefore, is identical to the treated plant with the exception of the presence of the endophyte and can serve as a control to detect the effects of the endophyte organism that is conferred on the plant. In some embodiments, the term "reference insulin plant" is used in this document to describe a reference plant that is genetically identical and subject to the same conditions, that is, a control plant, to the treated plant.
[00788] [00788] A "reference environment" refers to the environment, treatment or condition of the plant in which a measurement is made. For example, the production of a compound in a plant arranged heterologously to an endophyte organism can be measured in a reference environment of water stress and compared with the levels of the compound in an agricultural reference plant under the same conditions of use. uric stress. Alternatively, the levels of a compound in plants arranged heterologously to an endophytic organism and reference agricultural plant can be measured under identical stress-free conditions.
[00789] [00789] A "plant element" is intended to refer generically to an entire plant or a plant component, including, among others, tissues, parts and cell types of the plant. An element of the plant is preferably one of the following: whole plant, seedling, meristematic tissue, mo tissue, vascular tissue, dermal tissue, seed, leaf, root, bud, stem, flower, fruit, stem, bulb, tuber, corm, keikis, gomo. As used in this document, a "plant element" is synonymous with a "portion" of a plant and refers to any part of the plant, and can include different tissues and / or organs and can be used interchangeably with the term "fabric" throughout this document.
[00790] [00790] Similarly, a "plant reproductive element" is intended to refer generically to any part of a plant that is capable of initiating other plants through sexual or asexual reproduction of that plant, for example, but not limited to : seed, seedling, root, bud, cut, graft, graft, stalk, bulb, tuber, corm, kKkeikis or bud.
[00791] [00791] A "progeny seed", as used in this document, refers to the seed produced by a host plant that has been inoculated with, or discarded heterologously, an endophyte organism. For example, in the present invention, a seed, plant element or whole plant may become heterologously disposed to an endophyte organism, and the plant that is grown from said seed, or plant that is grown in heterologous association with said endophyte organism, can itself produce progeny seeds that comprise altered nutritional composition compared to seeds obtained from plants that have not been grown from a plant element heterologous to an endophyte organism or obtained from a plant (host ) parenting that has become heterologously disposed to an endophyte organism at some point in its life cycle. In the general sense, the phrase “progeny seed” can be interpreted as representing any plant propagation unit produced by the host plant that is capable of becoming another individual of that same plant species.
[00792] [00792] A "population" of plants refers to more than one plant, which are of the same taxonomic category, typically of the same species, and usually also share a common genetic derivation.
[00793] [00793] As used in this document, an “agricultural seed” is a seed used to grow a plant typically used in agriculture (an “agricultural plant”). The seeds can be from a monocot plant and can be planted to produce an agricultural product, for example, animal feed, food, fibers, fuels, industrial uses, etc. As used in this document, an agr Cola seed is a seed that is prepared for planting, for example, on farms for cultivation.
[00794] [00794] "Agricultural plants" or "plants of agronomic importance include plants that are grown by humans for food, feed, fiber, combustible and / or industrial purposes. In some embodiments, plants (including seeds and other plant elements) treated in accordance with the present invention are monocots. In some embodiments, the plants (including seeds or other plant elements) treated in accordance with the present invention are dicots. In some embodiments, plants treated in accordance with the present invention include, but are not limited to: agr Cola line, agr Cola grass plants or other field crops: wheat, rice, barley, buckwheat, beans (soybeans, peas -pie, dry), corn (grains, seeds, sweet corn, silage, popcorn, high oil), cotton, canola, peas (dry, succulent), peanuts, saffron, sunflower, alfalfa hay, forage (alfalfa, clover, vetch and clover), berries and small fruits (blackberries, blueberries, red currant, elderberry, currant, blueberry, blueberry, raspberry, strawberry, banana and grape), bulb crops (garlic, leeks, onions, shallots, shallots and ornamental bulbs), fruits and vegetables (citrus hybrids, grapefruits, kumquat, lines, oranges and pummels), pumpkin vegetables (cucumbers, melons, gourds, pumpkins and squashes), flowers, bedding, ornamentals, vegetables fru feros (eggplant, peppers and peppers, tomatillos and tomatoes), herbs, spices, mint, crops hydroponic (cucumbers, tomatoes, lettuce, herbs and spices), leafy vegetables and barley crops (arugula, celery, chervil, end ia, fennel, lettuce (head and leaf), parsley, radicchio, rhubarb, spinach, chard, broccoli, brussels sprouts, kale, cauliflower, kale, kale,
[00795] [00795] A "closely related variety" comprises a common genetic derivation with a variety of plants. In some embodiments, an closely related variety has at least one lineage of dual origin in common with the plant variety. In some embodiments, a closely related variety has at least one parental lineage in common with the plant variety. In some modalities, a closely related variety has at least 60%, 70%, 80%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5%, 99.7%, 99.9%, 99.99% of the same SNPs detected in the plant variety. In some embodiments, an closely related variety has at least 1, at least 2, at least 3, at least 4, at least 5, at least 7, at least 8, at least 9, at least 10, at least at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, or 20 or more of the same SNPs detected in the plant variety. In some embodiments, an closely related variety has at least 20, at least 30, at least 40, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 200 , at least 300, at least 400, at least 500, at least 600, at least 700, at least 800, at least 900, or 100 or more of the same SNPs detected in the plant variety. In some embodiments, a closely related variety has at least 1000, at least 2,000, at least 3,000, at least 4,000, at least 5,000, at least 7,000, at least 8,000, at least 9,000 or 10,000 or more than same SNPs detected in the plant variety. In some embodiments, a closely related variety has at least 10,000, at least 20,000, at least 30,000, at least 40,000, at least 60,000, at least 70,000, at least 80,000, at least 90,000 or 100,000 or more of those same SNPs detected in the plant variety.
[00796] [00796] A "synthetic composition" comprises one or more endophyte organisms combined by human effort with an element of the plant arranged in a heterologous way or a treatment formulation, the said combination that is not found in nature. In some embodiments, the term "synthetic composition" means one or more plant elements or formulation components combined by human effort with an isolated and purified endophyte organism composition. In some embodiments, said purified endophyte organism composition is mechanically or manually applied, artificially inoculated or arranged on a plant element in a way that is not found or on the plant element prior to application of the purified endophyte organism composition, for example , that combination or association that is not found in nature.
[00797] [00797] In some embodiments, "synthetic composition" used to refer to a treatment formulation comprising an isolated and purified population of endophyte organisms arranged heterologously to a plant element. In some embodiments, "synthetic composition" refers to a purified population of endophyte organisms in a treatment formulation comprising additional compositions with which said endophyte organisms are not found in nature.
[00798] [00798] A "treatment formulation" refers to a mixture of chemicals that facilitate the stability, storage and / or application of the endophyte organism compositions. The treatment formulations can comprise any one or more agents such as: surfactant, a buffer, an adhesion promoter, a microbial stabilizer, a fungicide, an anti-complex agent, a herbicide, a nematicide, an insecticide, a plant growth regulator , a rodenticide, a desiccant, a nutrient, an excipient, a wetting agent, a salt.
[00799] [00799] In some embodiments, a "carrier compatible with agriculture" can be used to formulate an agricultural formulation or other composition that includes a purified endophyte organism preparation. As used in this document, an "agricultural-compatible carrier" refers to any material, other than water, that can be added to a plant element without causing or having an adverse effect on the plant element (for example, reducing germination of the seeds) or the plant that grows from the plant element or similar.
[00800] [00800] "Plant health" is demonstrated by the presence or improvement of a trait of agronomic importance found in a plant or plant element compared to a reference plant or plant element. The compositions and methods described in this document may provide an enhanced "agronomic characteristic" or "agronomic importance characteristic" for a host plant, which include, but are not limited to disease resistance, drought tolerance, heat tolerance, tolerance cold, salinity tolerance, metal tolerance, herbicide tolerance, improved water use efficiency, improved nitrogen utilization, improved nitrogen fixation, pest resistance, herb resistance, pathogen resistance, yield improvement, improvement of yield health, enhancing vigor, enhancing growth, enhancing photosynthetic capacity, enhancing nutrition, altered protein content, altered oil content, increased biomass, increased shoot length, increased root length, increased root area, architecture improved root system, modulation of a metabolite, modulation of the proteome, increased weight seed, altered seed carbohydrate composition, altered seed carbohydrate composition, altered seed oil composition,
[00801] [00801] In some modalities, a treatment is arranged in a heterologous way in an element of the plant in an effective amount to improve a trait of agronomic importance. In some modalities, treatments capable of improving plant health are applied in an effective amount to improve a trait of agronomic importance or tolerance by at least 0.1%, at least 0.5%, at least 1%, at least 2%, at least 3%, between 3% and 5%, at least 5%, between 5% and 10%, at least 10%, between 10% and 15%, for example, at least 15%, between 15% and 20%, at least 20%, between 20% and 30%, at least 30%, between 30% and 40%, at least 40%, between 40% and 50%, at least 50%, between 50% and 60 %, at least 60%, between 60% and 75%, at least 75%, between 75% and 100%, at least 100%, between 100% and 150%, at least 150%, between 150% and 200%, at least 200%, between 200% and 300%, at least 300% or more, compared to an element of the reference plant that does not additionally comprise said endophyte organism.
[00802] [00802] In some modalities, an improvement in a trait of agronomic importance is measured by the "gain rate". The victory rate is the proportion of repetitions in which the treatment shows an improvement in a trait of agronomic importance in relation to the reference replicas. In some embodiments, the replicas are individual plants. In some modalities, replicates are plots, for example, plots replicated within a randomized, complete, field test. In some modalities, replicas are field trials conducted in different geographies.
[00803] [00803] In some modalities, the endáophyte organism is able to improve a trait of agronomic importance in concentrations detected in the treated plant element or at least 10 º 2 CFU or spores per plant element, between 10º 2 and 10º 3 CFU or spores per plant element, about 10º 3 CFU or spores per plant element, between 10º 3 and 10º 4 CFU or spores per plant element, about 10 * 4 CFU or spores per plant element, or between 10º 4, of about 10º 5 CFU or spores per plant element, at least 10º 5 CFU or spores per plant element, between 10º 5e
[00804] [00804] The term "characteristic of nutritional quality" includes any measurable parameter of a seed that directly or indirectly influences the value (nutritional or economic) of said seed, for example, but not limited to: proteins, fats, carbohydrates , ash, moisture, fiber and calories. In some cases, “trait of nutritional quality” is synonymous with “trait of nutritional quality” or “trait of nutritional quality of seeds”, and can refer to any composition of the associated plant element, more particularly compositions providing benefits to others organisms that consume or use that plant element. As used in this document, “oil” and “fat” are used interchangeably.
[00805] [00805] An increased "seed yield" can refer to any increase in weight, size or abundance of seeds or fruits per unit of measure, for example, per plant, by number of plants, by mass of plants, per acre planted , per acre collected. In some modalities, seed yield is reported as pounds or bushels of seeds produced per collected acre. The terms seed and grain are used interchangeably in this document. The yield can also refer to the recovery of a particular component of a plant tissue by processing, for example, the amount of oil that can be extracted from the seed unit. Typically, the Btica character in particular is designated when referring to increased yield, for example, increased seed yield or increased oil yield. Where characteristic 6 is not specified, it can be assumed that yield refers to seed yield and the terms can be used interchangeably.
[00806] [00806] As used in this document, the terms “low water condition” and “dry condition”, or “little water” and “dry”, can be used interchangeably. For example, a method or composition for improving a plant's ability to grow in dry conditions means the same as its ability to grow in conditions with little water. In such cases, it can be said that the plant has improved tolerance to water stress.
[00807] [00807] As used in this document, the terms "normal watering" and "well watered" are used interchangeably to describe a plant grown under typical growing conditions without water restriction.
[00808] [00808] In addition, "altered metabolic function" or "altered enzymatic function" may include, but is not limited to: altered auxin production, altered nitrogen fixation, altered production of an antimicrobial compound, altered production of a siderophore, alteration of the phosphate solubilization mineral, altered production of a cellulase, altered production of a chitinase, altered production of a xylanase, altered production of aceto ha, altered use of a carbon source.
[00809] [00809] "Nutrient" or "seed nutrient" refers to any composition of the associated plant element, more particularly compositions that provide bio benefits to other organisms that consume or use said plant element.
[00810] [00810] "Potential of agronomic trait" means the ability of an element of the plant to exhibit a phenotype, preferably an improved agronomic trait, at some point in its life cycle, or to transport that phenotype to another element of the plant to which it is located associated in the same plant. For example, a plant element can comprise an endophyte organism that will provide benefit to the leaf tissue of a plant from which the plant element is grown; in such a case, the plant element comprising that endophyte organism has the potential for an agronomic trait for a particular phenotype (for example, increased biomass in the plant) even if the plant element itself does not exhibit that phenotype.
[00811] [00811] In some cases, the present invention contemplates the use of compositions that are "compatible" with agricultural chemicals, including, but not limited to, a fungicide, an anti-complex compound, a bactericide, a virucide, a herbicide, a nematicide , a parasiticide, a pesticide or any other agent widely used in agriculture that has the effect of killing or otherwise interfering with the optimal growth of another organism. As used in this document, a composition is "compatible" with an agricultural chemical when the organism is modified, such as by genetic modification, for example, contains a transgene that confers resistance to an herbicide, or is adapted to grow or survive the concentration of the agricultural chemical used in agriculture. For example, an endophyte organism disposed on the surface of a plant element is compatible with the fungicide metalaxyl if it is able to survive the concentrations that are applied on the surface of the plant element.
[00812] [00812] As used in this document, a “colony forming unit” (“CFU”) is used as a measure of viable microorganisms in a sample. A CFU is an individual viable cell capable of forming a visible colony in a solid medium whose individual cells are derived by cell division of a parental cell.
[00813] [00813] The terms "decreased", "less", "slower" and "increased", "faster", "enhanced", "greater" as used in this document refer to a decrease or increase in a characteristic of the plant element treated with the resulting endophyte organism or plant compared to an untreated plant element or resulting plant. For example, a decrease in a characteristic can be at least 1%, at least 2%, at least 3%, at least 4%, at least 5%, between 5% and 10%, at least 10%, between 10% and 20%, at least 15%, at least 20%, between 20% and 30%, at least 25%, at least 30%, between 30% and 40%, at least 35%, at least 40%, between 40% and 50%, at least 45%, at least 50%, between 50% and 60%, at least about 60%, between 60% and 70%, between 70% and 80%, at least 75%, at least about 80%, between 80% and 90%, at least about 90%, between 90% and 100%, at least 100%, between 100% and 200%, at least 200%, at least about 300 %, at least about 400% or more below the untreated control and an increase can be at least 1%, at least 2%, at least 3%, at least 4%, at least 5%, between 5% and 10%, at least 10%, between 10% and 20%, at least 15%, at least 20%, between 20% and 30%, at least 25%, at least 30%, between 30% and 40%, at least least 35%, at least 40%, between 40% and 50%, at least 45%, by at least 50%, between 50% and 60%, at least about 60%, between 60% and 70%, between 70% and 80%, at least 75%, at least about 80%, between 80% and 90 % at least about
[00814] [00814] As used in this document, a microbe or plant or plant element is "modified" when it comprises an artificially introduced genetic or epigenetic "modification". In some embodiments, the modification is introduced by a genome manipulation technology. In some embodiments, the modification is introduced by a targeted nuclease. In some embodiments, targeted nucleases include, but are not limited to, nuclease with transcriptional activator effector (TALEN), zinc finger nuclease (ZNF), short intergrated regulated interspatial palindromic repetitions (CRISPR), CRISPR / Cas9, CRISPR / CPF1 and combinations thereof. In some embodiments, the modification is an epigenetic modification. In some embodiments, the modification is introduced by treatment with a DNA methyltransferase inhibitor, such as 5-azacytidine, or a histone deacetylase inhibitor, such as 2-amino-7-methoxy-3H-phenoxazin-3-one. In some embodiments, the modification is introduced via tissue culture. In some embodiments, a modified microbe or plant or plant element comprises a transgene.
[00815] [00815] The endophyte organisms described in this document provide several unexpected and significant advantages to agricultural plants over other plant-associated microbes, as demonstrated in the Examples.
[00816] [00816] In some modalities, the endophyte organism is selected from Table 4. In some modalities, the endophyte organism is selected from Table 7.
[00817] [00817] In some embodiments, the endophyte organism comprises a polynucleotide sequence that is at least 97% identical to at least one selected sequence from the group consisting of SEQ ID NOs: 32 and 33. In some embodiments, the endophyte organism comprises a polynucleot sequence that is between 97% and 98% identical, at least 98% identical, between 98.0% identical and 99.5% identical, or at least 99.5% identical or 100% identical to at least one sequence selected from the group consisting of SEQ ID NOs: 32 and 33. In some embodiments, the endophyte organism comprises two polynucleotide sequences that are between 97% and 98% identical, at least 98% identical, between 98.0% identical and 99.5% identical, or at least 99.5% identical or 100% identical to the sequences SEQ ID NOs: 32 and 33. In some embodiments, the endophyte organism is a macrospinous periconia. In some embodiments, the endophyte is deposited with the Agricultural Research Service Culture Collection (NRRL), United States Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604, under the terms of the Budapest Treaty, as Deposit ID: NRRL-67466. In some embodiments, the endophyte organism is deposited under NRRL Culture Deposit No. NRRL-67466, and then modified. In some embodiments, the modified endophyte organism derived from the endophyte organism deposited as NRRL Culture Deposit No. NRRL-67466 maintains the ability to modulate the nutrient composition of a seed produced by a plant element heterologously arranged with the modified endophyte organism.
[00818] [00818] In some embodiments, the endophyte organism comprises a polynucleotide sequence that is at least 97% identical to at least one sequence selected from the group consisting of SEQ ID NOs: 34, 35, 36, 37 and 38. In some modalities, the endophyte organism comprises a polynucleotide sequence that is between 97% and 98% identical, at least 98% identical, between 98.0% identical and 99.5% identical, or at least 99.5% identical or 100% identical to at least one sequence selected from the group consisting of SEQ ID NOs: 34, 35, 36, 37 and 38. In some embodiments, the endophyte organism comprises at least two polynucleotide sequences that are at least 97% identical to at least at least two sequences selected from the group consisting of SEQ ID NOs: 34, 35, 36, 37 and 38. In some embodiments, the endophyte organism comprises at least two polynucleotide sequences that are between 97% and 98% identical, at least 98% identical, between 98.0% identical and 99.5% identical or at least 99.5% identical or 100% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 34, 35, 36, 37 and 38. In some embodiments, the endophyte organism comprises at least three hint polynucleot sequences that are at least 97% identical to at least three sequences selected from the group consisting of SEQ ID NOs: 34, 35, 36, 37 and 38. In some embodiments, the endophyte organism comprises at least three polynucleot sequences hints that are between 97% and 98% identical, at least 98% identical, between 98.0% identical and 99.5% identical, or at least 99.5% identical or 100% identical to at least three sequences selected from the group consisting of SEQ ID NOs: 34, 35, 36, 37 and 38. In some embodiments, the endophyte organism comprises at least four polynucleotide sequences that are at least 97% identical to at least four sequences selected from the group consisting of in SEQ ID NOs: 34, 35, 36, 37 and 38. In some embodiments, the endophyte organism comprises at least four polynucleotide sequences that are between 97% and 98% identical, at least 98% identical, between 98.0% identical and 99.5% identical, or at least 99.5% identical or 100% identical to at least four sequences selected from the group consisting of SEQ ID NOs: 34, 35, 36, 37 and 38. In some embodiments, the endophyte organism comprises at least five polynucleotide sequences that are at least 97 % identical to at least five sequences selected from the group consisting of SEQ ID NOs: 34, 35, 36, 37 and 38. In some embodiments, the endophyte organism comprises at least five polynucleotide sequences that are between 97% and 98% identical, at least 98% identical, between 98.0% identical and 99.5% identical, or at least 99.5% identical or 100% identical to at least five sequences selected from the group consisting of SEQ ID NOs: 34 , 35, 36, 37 and 38. In some modalities, the body endophyte is a Curvularia spicifera. In some embodiments, the endophyte is deposited with the Agricultural Research Service Culture Collection (NRRL), United States Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604, under the terms of the Budapest Treaty, as Deposit ID: NRRL-67467. In some modalities, the endophyte organism is an endophyte organism that is deposited as NRRL Culture Deposit No. NRRL-67467 and later modified. In some embodiments, the modified endophyte organism derived from the endophyte organism deposited as NRRL Culture Deposit No. NRRL-67467 maintains the ability to modulate the nutrient composition of a seed produced by a plant element heterologously arranged with the modified endophyte organism.
[00819] [00819] In some embodiments, the endophyte organism comprises a polynucleotide sequence that is at least 97% identical to SEQ ID NO: 39. In some embodiments, the endophyte organism comprises a polynucleotine sequence that is between 97% and 98% identical , at least 98% identical, between 98.0% identical and 99.5% identical, and at least 99.5% identical or 100% identical to SEQ ID NO: 39. In some embodiments, the endophyte is an Aspergillus ruber .
[00820] [00820] In some embodiments, the endophyte organism comprises a polynucleotide sequence that is at least 97% identical to at least one sequence selected from the group consisting of SEQ ID NOs: 40, 41,42 and
[00821] [00821] In some embodiments, the endophyte organism comprises a polynucleotide sequence that is at least 97% identical to at least one sequence selected from the group consisting of SEQ ID NOs: 44, 45, 46, 47 and 48. In some modalities, the endophyte organism comprises a polynucleotide sequence that is between 97% and 98% identical, at least 98% identical, between 98.0% identical and 99.5% identical, at least 99.5% identical or 100% identical at least one sequence selected from the group consisting of SEQ ID NOs: 44, 45, 46, 47 and 48. In some embodiments, the endophyte organism comprises at least two polynucleotide sequences that are at least 97% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 44, 45, 46, 47 and 48. In some embodiments, the endophyte organism comprises at least two polynucleotide sequences that are between 97% and 98% identical, at least 98 % identical, between 98.0% identical and 99.5% identical s, or at least 99.5% identical or 100% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 44, 45, 46, 47 and 48. In some embodiments, the endophyte organism comprises at least three hint polynucleot sequences that are at least 97% identical to at least three sequences selected from the group consisting of SEQ ID NOs: 44, 45, 46, 47 and 48. In some embodiments, the endophyte organism comprises at least three polynucleot sequences hints that are between 97% and 98% identical, at least 98% identical, between 98.0% identical and 99.5% identical, or at least 99.5% identical or 100% identical to at least three sequences selected from the group consisting of SEQ ID NOs: 44.45, 46, 47 and 48. In some embodiments, the endophyte organism comprises at least four polynucleotide sequences that are at least 97% identical to at least four sequences selected from the group consisting of SEQ ID NOs: 44, 45, 46, 47 and 48. In the In some embodiments, the endophyte organism comprises at least four polynucleotide sequences that are between 97% and
[00822] [00822] In some embodiments, the endophyte organism comprises a polynucleotide sequence that is at least 97% identical to at least one sequence selected from the group consisting of SEQ ID NOs: 49, 50 and 51. In some embodiments, the organism endophyte comprises a polynucleotide sequence that is between 97% and 98% identical, at least 98% identical, between 98.0% identical and 99.5% identical, at least 99.5% identical or 100% identical to at least one sequence selected from the group consisting of SEQ ID NOs: 49, 50 and 51. In some embodiments, the endophyte organism comprises at least two polynucleotide sequences that are at least 97% identical to at least two sequences selected from the group consisting of in SEQ ID NOs: 49, 50 and 51. In some embodiments, the endophyte organism comprises at least two polynucleotide sequences that are between 97% and 98% identical, at least 98% identical, between 98.0% identical and 99, 5% identical, or at least 99.5% i identical or 100% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 49, 50 and 51. In some embodiments, the endophyte organism comprises at least three polynucleotide sequences that are at least 97% identical to at least at least three sequences selected from the group consisting of SEQ ID NOs: 49, 50 and 51. In some embodiments, the endophyte is an Enterobacter cowanii. In some modalities, the endophyte organism is deposited with the Agricultural Research Service Culture Collection (NRRL), at the United States Department of Agriculture
[00823] [00823] In some cases, the endophyte organism, or one or more components thereof, is of monocional origin, providing high genetic uniformity of the population of endophyte organisms in an agricultural formulation or within a plant element or synthetic combination with the endophyte organism.
[00824] [00824] In some embodiments, the endophyte organism can be grown in a culture medium or it can be adapted to culture in a culture medium.
[00825] [00825] The synthetic compositions provided in this document are preferably stable. The endophyte organism can be stable in storage, where at least 0.01% of CFU are viable after storage in desiccated form (ie, moisture content of 30% or less) to 1, 2,3,4,5,6 , 7,8,9,10 or more than 10 weeks at 4 C or room temperature. Optionally, a long-lasting formulation is in a dry formulation, in a powder formulation or in a lyophilized formulation. In some embodiments, the formulation is formulated to provide stability for the population of endophyte organisms. In one embodiment, the formulation is substantially stable at temperatures between about -20 "C and about 50 ° C for at least about 1, 2, 3, 4, 5 or 6 days, or 1, 2, 3 or 4 weeks, or 1, 2,3,4,5,6,7,8,9, 10, 11 or 12 months, or one or more years. In another embodiment, the formulation is substantially stable at temperatures between about 4 "C and about 37" C for at least about 5, 10, 15, 20, 25, 30 or more than 30 days.
[00826] [00826] It is contemplated that synthetic methods and compositions can be used to improve a characteristic of agronomic importance to a plant.
[00827] [00827] The methods described in this document can also be used with transgenic plants comprising one or more exogenous transgenes, for example, to produce additional trace benefits conferred by the newly introduced endophytic microbes.
[00828] [00828] For example, the endophyte organism can provide a benefit or improved tolerance to a plant that is at least 3%, between 3% and 5%, at least 5%, between 5% and 10%, at least 10 %, between 10% and 15%, for example, at least 15%, between 15% and 20%, at least 20%, between 20% and 30%, at least 30%, between 30% and 40%, at least 40%, between 40% and 50%, at least 50%, between 50% and 60%, at least 60%, between 60% and 75%, at least 75%, between 75% and 100%, at least 100% , between 100% and 150%, at least 150%, between 150% and 200%, at least 200%, between 200% and 300%, at least 300% or more, when compared to uninoculated plants grown under the same conditions.
[00829] [00829] In one embodiment, it is contemplated that the plant of the present invention is rice (Oryza spp.), In particular O. sativa and O. glaberrima, and members of the main subspecies of O. sativa japonica, javanica and indica. In some embodiments, the present invention contemplates the use of endophyte organisms that can confer a trace of agronomic benefit on an element of the rice plant or rice plant to which it is heterologously disposed.
[00830] [00830] In one embodiment, it is contemplated that the plant of the present invention is corn (Zea spp.), In particular Zea mays ssp., Such as Zea mays indenata, Zea mays indurata, Zea mays amylacea, Zea mays saccharata and Zea mays everta.
[00831] [00831] In one embodiment, it is contemplated that the plant of the present invention is wheat (Triticum spp.), Including T. aestivum and T. durum species and varieties including hard winter red (HRW), hard spring red (HRS) , hard white (HW), durum, soft white (SW) and soft winter red (SRW). In some embodiments, the present invention contemplates the use of endophyte organisms that can confer a trace of agronomic benefit on an element of the wheat plant or wheat plant to which it is heterologously arranged.
[00832] [00832] In one embodiment, it is contemplated that the plant of the present invention is soy (Glycine max). In some embodiments, the present invention contemplates the use of endophyte organisms that can confer a trace of agronomic benefit on an element of the soybean plant or soybean plant to which it is heterologously disposed.
[00833] [00833] In one embodiment, it is contemplated that the plant of the present invention is cotton (genus Gossypium, including species such as Gossypium arboretum, Gossypium herbaceum, Gossypium hirsutum, Gossypium barbadense). In some embodiments, the present invention contemplates the use of endophyte organisms that can confer a trace of agronomic benefit on an element of the cotton plant or cotton plant to which it is heterologously disposed.
[00834] [00834] In some embodiments, the plant elements of the present invention include wild plants and domesticated varieties of the genera Zea, Triticum, Glycine, Gossypium. Plant elements can be developed by any technique, including, but not limited to, targeted evolution, selection, marker-assisted selection, hybridization, crossing, backcrossing, reproduction, polyploidization, reverse reproduction, duplicated haploids, induced mutation, other genetic or epigenetic modifications and combinations of these.
[00835] [00835] In some cases, the endophyte organisms described in this document are able to pass from one type of tissue to another. For example, the detection and isolation of endophyte organisms of the present invention in mature plant tissues after coating a plant element on the outside demonstrates its ability to move from the plant element to the vegetative tissues of a maturing plant. . Therefore, in some embodiments, the endophyte population is able to move from outside the plant element to the vegetative tissues of a plant. In some embodiments, the endophyte that is coated on the plant element of a plant is capable, after the germination of the plant element in a vegetative state, to be located in a different tissue of the plant. For example, endophytes may be able to locate any of the plant's tissues, including: the root, advent Cia root, seminal root, root, bud, leaf, flower, bud (tassel), tassel, meristem, pollen, pistil , ovaries, stamen, fruit, stolon, rhizome, nodule, tuber, trichome, guard cells, hydatode, petal, sepal, gluma, raque, vascular exchange, phloem and xylem. In one embodiment, the endophyte is able to locate itself in the root and / or hairs of the plant. In another modality, the endáophyte is able to locate itself in photosynthetic tissues, for example, leaves and shoots of the plant. In other cases, the endophyte is located in the vascular tissues of the plant, for example, in the xylem and phloem. In yet another modality, the endophyte is able to be located in the reproductive tissues (flower, pollen, pistil, ovary, stamen, fruit) of the plant. In another modality, the endáophyte is able to locate itself in the root, shoots, leaves and reproductive tissues of the plant. In yet another embodiment, the endophyte colonizes a tissue from the plant's fruit or plant element. In yet another embodiment, the endophyte is able to colonize the plant so that it is present on the surface of the plant (that is, its presence is detectable present outside the plant or in the plant's episphere). In still other embodiments, the endophyte is able to be located substantially in all or all tissues of the plant. In certain embodiments, the endophyte is not located at the root of a plant. In other cases, the endophyte is not located in the photosynthetic tissues of the plant.
[00836] [00836] In some cases, endophytes are able to replicate within the host plant and colonize the plant.
[00837] [00837] The endophyte populations described in this document are capable of colonizing a host plant. Successful colonization can be confirmed by detecting the presence of the endophytic population within the plant. For example, after applying the endophyte to plant elements, high titers of the endophyte can be detected in the roots and shoots of plants that germinate from the plant elements. Detecting the presence of the endophyte inside the plant can be performed by measuring the viability of the endophyte after sterilization of the surface of the plant or plant element: colonization of the endophyte results in an internal location of the endophyte, making it resistant to the conditions sterilization of the surface. The presence and amount of endophytes can also be established using other means known in the art, for example, immunofluorescence microscopy using antibodies specific for microbes or fluorescence in situ hybridization. Alternatively, specific nucleic acid probes that recognize conserved sequences from an endophyte can be employed to amplify a region, for example by quantitative PCR, and correlated with CFUs using a standard curve.
[00838] [00838] In another modality, the endophyte is heterologously disposed, for example, on the surface of a reproductive element of an agr Cola plant, in an amount effective to be detectable in the mature agrola plant. In some embodiments, the endophyte is heterologously disposed in an amount effective to be detectable in an amount of at least about 100 CFU, between 100 and 200 CFU, at least about 200 CFU, between 200 and 300 CFU, at least about 300 CFU, between 300 and 400 CFU, at least about 500 CFU, between 500 and 1,000 CFU, at least about 1,000 CFU, between 1,000 and 3,000 CFU, at least about 3,000 CFU, between 3,000 and 10,000 CFU, at least about 10,000 CFU, between 10,000 and 30,000 CFU, at least about 30,000 CFU, between 30,000 and 100,000 CFU, at least about 100,000 CFU or more in the mature agr Cola plant.
[00839] [00839] In some cases, the endophyte is able to colonize certain plant elements or types of plant tissues. In one embodiment, the endophyte is heterologously disposed in the plant element or changes in an effective amount to be detectable within a target tissue of the mature agricultural plant selected from a fruit, seed, leaf, root or part of it. For example, the endophyte can be detected in an amount of at least about 100 CFU, at least about 200 CFU, at least about 300 CFU, at least about 500 CFU, at least about 1,000 CFU, at least about 3,000 CFU, at least about 10,000 CFU, at least about 30,000 CFU, at least about 100,000 CFU or more, in the target tissue of the mature agricultural plant.
[00840] [00840] In certain embodiments, the endophyte is selected based on its compatibility with commonly used agrochemicals. As described in this document, plants, particularly agricultural plants, can be treated with a wide range of agrochemicals, including fungicides, biocides (anti-complex agents), herbicides, insecticides, nematicides, rodenticides, bactericides, virucides, fertilizers and other agents.
[00841] [00841] In some modalities, endophytes exhibit tolerance to an agrochemical selected from the group consisting of: AerisO &, Avicta & DuoCot 202, CruiserO, Syntenta CCBO (A), Clariva6, Albaugh, DynastyO, ApronO, MaximO, Gaucho, Provoke & ST , Syngenta CCBGO, Trilexêe, WG Purple, WG Silver,
[00842] [00842] In some cases, it may be important for the endophyte to be compatible with agrochemicals, particularly those with anti-complex properties, in order to persist in the plant, although, as described in this document, there are many of these anti-complex agents that do not penetrate the plant, at least in a concentration sufficient to interfere with the endophyte. Therefore, when a systemic anti-complex agent is used in the plant, the compatibility of the endophyte to be inoculated with such agents will be an important criterion.
[00843] [00843] In one embodiment, natural isolates of endophytes that are compatible with agrochemicals can be used to inoculate the plants according to the methods described in this document. For example, endophytes that are compatible with anti-complex agents used in agriculture can be isolated by placing an endophyte culture in a Petri dish comprising an effective concentration of the anti-complex agent and isolating endophyte colonies compatible with the anti-complex agent. In another embodiment, an endophyte that is compatible with an anti-complex agent is used for the methods described in this document.
[00844] [00844] Endophytes compatible with bactericides can also be isolated by medium selection | liquid. The culture of endophytes can be plated in petri dishes without any form of mutagenesis; alternatively, endophytes can be mutagenized using any means known in the art. For example, endophyte cultures can be exposed to UV light, gamma irradiation or chemical mutagenic agents, such as ethyl methanesulfonate (EMS), ethidium bromide (EtBr) dichlorvos (DDVP, methyl methane sulfonal (MMS), triethyl phosphate (TEP) , trimethylphosphate (TMP)) nitrous acid or DNA base analogs, before selection in media comprising fungicides. Finally, when the mechanism of action of a specific bactericide is known, the target gene can be mutated specifically (by gene deletion, gene replacement, mutation directed at the uncle etc.) to generate an endophyte that is resilient against that product in particular. Note that the methods described above can be used to isolate endophytes that are compatible with both bacteriostatic and bactericidal compounds.
[00845] [00845] It will also be appreciated by one skilled in the art that a plant can be exposed to various types of anti-complex compounds, simultaneously or in succession, for example, at different stages of plant growth. Where the target plant is likely to be exposed to multiple anti-complex agents, an endophyte that is compatible with many or all of these agrochemicals can be used to inoculate the plant. An endophyte that is compatible with several agents can be isolated, for example, by serial selection. An endophyte compatible with the first agent can be isolated as described above (with or without prior mutagenesis). A culture of the resulting endophyte can then be selected for its ability to grow in liquid or solid media comprising the second agent (again, with or without prior mutagenesis). Colonies isolated from the second selection are then tested to confirm their compatibility for both agents.
[00846] [00846] Likewise, endophytes that are compatible with biocides (including herbicides such as glyphosate or anti-complex compounds, whether bacteriostatic or bactericidal) that are employed in agriculture can be isolated using methods similar to those described to isolate compatible endophytes. In some modalities, mutagenesis of the endophyte population can be performed before selection with an anti-complex agent. In another modality, the selection is carried out in the population of endophytes without previous mutagenesis. In yet another embodiment, serial selection is carried out in an endophyte: the endophyte is first selected for compatibility with a first anti-complex agent. The isolated compatible endophyte is then cultured and selected for compatibility with the second anti-complex agent. Any colony so isolated is tested for compatibility with each or both of the anti-complex agents to confirm compatibility with these two agents.
[00847] [00847] Compatibility with an antimicrobial agent can be determined by several means known in the art, including comparison of the maximum inhibitory concentration of unmodified and modified endophytes. In some embodiments, the present invention discloses an isolated modified endophyte, in which the endophyte is modified to exhibit at least 3 times more, for example, at least 5 times more, between 5 and 10 times more, at least 10 times more , between 10 and 20 times more, at least 20 times more, between 20 and 30 times more, at least 30 times more or more inhibitory concentration minima to an antimicrobial agent when compared to the unmodified endophyte.
[00848] [00848] In a specific modality, endophytes with improved compatibility with the herbicide glyphosate are disclosed in this document. In some embodiments, the endophyte has a doubling time in growth medium comprising at least 1 mM glyphosate, for example, between 1 mM and 2 mM glyphosate, at least 2 mM glyphosate, between 2 MM and 5 mM glyphosate, at least 5 mM glyphosate, between 5 mM and 10 mM glyphosate, at least 10 mM glyphosate, between 10 mM and 15 mM glyphosate, at least 15 mM glyphosate or more, which is no more than 250%, between 250% and 100%, for example, not more than 200%, between 200% and 175%, not more than 175%, between 175% and 150%, not more than 150%, between 150 % and 125%, or not more than 125%, of the endophyte doubling time in the same growth medium that does not comprise any glyphosate. In a particular embodiment, the endophyte has a doubling time in the growth medium comprising glyphosate to SMM which is not more than 150% of the doubling time of the endophyte in the same growth medium that does not comprise glyphosate.
[00849] [00849] In another embodiment, the endophyte has a doubling time in a plant tissue comprising at least 10 ppm of glyphosate, between 10 and ppm, for example, at least 15 ppm of glyphosate, between at least 15 and 10 ppm 20 ppm glyphosate, between 20 and 30 ppm, at least 30 ppm glyphosate, between 30 and 40 ppm, at least 40 ppm glyphosate or more, which is not more than 250%, between 250% and 200%, for example, no more than 200%, between 200% and 175%, no more than 175%, between 175% and 150%, no more than 150%, between 150% and 125%, or no more than 125%, of the time duplication of the endophyte in a reference plant tissue that does not comprise glyphosate. In a particular embodiment, the endophyte has a doubling time in a plant tissue comprising 40 ppm glyphosate which is not more than 150% of the doubling time of the endophyte in a reference plant tissue that does not comprise any glyphosate.
[00850] [00850] The selection process described above can be repeated to identify endophyte isolates that are compatible with a multiplicity of agents.
[00851] [00851] Candidate isolates can be tested to ensure that selection for compatibility with agrochemicals does not result in loss of the desired bioactivity. Endophyte isolates that are compatible with commonly used agents can be selected as described above. The resulting compatible endophyte can be compared with the parental endophyte in plants in terms of their ability to promote germination.
[00852] [00852] Endophytes compatible with agrochemicals generated as described above can be detected in samples. For example, when a transgene was introduced to make the endophyte compatible with the agrochemical (s), the transgene can be used as a target gene for PCR amplification and detection. In addition, when point mutations or deletions for a portion of a specific gene or several genes result in compatibility with the agrochemical (s), single point mutations can also be detected by PCR or other means known in the art. Such methods allow the detection of the endophyte, even if it is no longer viable. Thus, plant commodity products produced using the endophytes compatible with agrochemicals described in this document can be easily identified using these and related nucleic acid detection methods.
[00853] [00853] The present invention contemplates the establishment of a relationship between a symbiont and an element of the plant. In some embodiments, the association of endophytes results in a detectable change in the plant element or the entire plant. The detectable change can be an improvement in several agronomic traits (for example, improved general health, increased response to biotic or abiotic stresses or increased properties of the plant or a plant element, including fruits and grains). Alternatively, the detectable change can be a physiological or biological change that can be measured by methods known in the art. Detectable changes are described in more detail in the sections below. As used in this document, an endophyte is considered to have conferred an improved agr Cola trait, regardless of whether the improved trait arose from the plant, the endophyte or the combined action between the plant and the endophyte. Therefore, for example, if a beneficial hormone or chemical product is produced by the plant or by the endophyte, for purposes, the endophyte will be considered to have conferred an improved agricultural trait to the host plant, compared to an isoline plant that has not been heterologously disposed to said endophyte.
[00854] [00854] In some modalities, provided in this document, there are methods to produce an element of the plant from a plant with a hereditarily altered trace. The trait of the plant can be changed without known genetic modification of the plant's genome, and comprises the following steps. First, an isolated endophyte preparation that is heterologously disposed to the plant element of the plant is provided and optionally processed to produce an endophyte formulation. The endophyte formulation is then contacted with the plant. The plants are then left to sow and the seeds are collected.
[00855] [00855] Also described in this document are plants and plant fields that are heterologously disposed to beneficial endophytes, in such a way that the general fitness, productivity or health of the plant or a portion of it, is maintained, increased and / or improved over a period of time. Improvement in the overall health of the plant can be assessed using various physiological parameters including, but not limited to height, total biomass, root and / or sprout biomass, emergence, seed germination, seedling survival, photosynthetic efficiency, transpiration rate , number of seeds / fruits or mass, grain yield, leaf chlorophyll content, photosynthetic rate, root length or any combination of these.
[00856] [00856] In some cases, a plant resulting from seeds or other elements of the plant treated with an endophyte may show a physiological change, as a compensation for the stress-induced reduction in photosynthetic activity. FW / Fm tests whether or not plant stress affects the | 1l photosystem in an adapted dark state. FvW / Fm is one of the most used chlorophyll fluorescence measurement parameters. The Fv / Fm test is designed to allow the maximum amount of light energy to follow the fluorescence path.
[00857] [00857] In some embodiments, the plants comprise endophytes capable of increasing heat and / or drought tolerance in sufficient quantity, such that increased growth or improved wilting recovery is observed under heat or drought stress conditions . For example, an endophyte population described in this document may be present in sufficient quantity in a plant, resulting in increased growth compared to a plant that does not contain endophytes, when grown under drought or thermal shock conditions, or following such conditions. The increase in tolerance to heat and / or drought can be evaluated with physiological parameters, including, among others, height increase, general biomass, biomass of shoots and / or shoots, seed germination, seedling survival, photosynthetic efficiency , transpiration rate, number of seeds / fruits or mass, grain or fruit yield of plants, leaf chlorophyll content, photosynthetic rate, length of leaves, recovery from wilting, turgor pressure or any combination of these, in comparison with a reference agricultural plant grown under similar conditions. For example, the endophyte can provide an improved benefit or tolerance to a plant that is at least 3%, between 3% and 5%, at least 5%, between 5% and 10%, at least 10%, between 10 % and 15%, for example, at least 15%, between 15% and 20%, at least 20%, between 20% and 30%, at least 30%, between 30% and 40%, at least 40%, between 40% and 50%, at least 50%, between 50% and 60%, at least 60%, between 60% and 75%, at least 75%, between 75% and 100%, at least 100%, between 100% and 150%, at least 150%, between 150% and 200%, at least 200%, between 200% and 300%, at least 300% or more, when compared to uninoculated plants grown under the same conditions.
[00858] [00858] In various embodiments, the endophytes arranged heterologously in the plant can confer various benefits to the plant, including but not limited to: thermal tolerance, herbicide tolerance, drought resistance, insect resistance, fungus resistance, resistance to viruses, bacteria resistance, male sterility, cold tolerance, salt tolerance, increased yield, greater efficiency in the use of nutrients, increased efficiency in the use of nitrogen, increased protein content, increased fermentable carbohydrate content, reduced lignin content, increased antioxidant content, increased water use efficiency, increased germination, increased biomass, altered root-to-sprout biomass ratio, improved soil water retention or a combination of these. A difference between the inoculated plant (for example, a plant to which one or more endophytes were heterologously disposed) and a reference agricultural plant can also be measured using other methods known in the art.
[00859] [00859] The endophyte populations described in this document are intended to be useful in the improvement of agricultural plants and, as such, can be formulated with other compositions as part of a carrier compatible with agriculture. It is considered that such carriers may include, but are not limited to: seed treatment, washing of trees, soaking of seedlings, leaf application, soil inoculum, furrow application, application in side cover, soil pretreatment, wound inoculation, drip irrigation, vector mediation by means of a pollinator, injection, osmoconditioning, hydroponics, aquaponics, aeroponics. The carrier composition with endophyte populations can be prepared for agricultural application as a formulation | liquid, solid or gaseous. Application to the plant can be achieved, for example, as a powder for surface deposition on plant leaves, as a spray for the entire plant or selected plant element, as part of a drip on the soil or roots, or as a coating in the plant element before planting. Such examples are intended to be illustrative and not to limit the scope of the invention.
[00860] [00860] The formulation useful for these embodiments generally and typically includes at least one member selected from the group consisting of a buffer, a tackifier, a microbial stabilizer, a fungicide, an anti-complex agent, a herbicide, a nematicide, a insecticide, bactericide, virucide, plant growth regulator, rodenticide, desiccant and nutrient.
[00861] [00861] The carrier can be a solid carrier or carrier | liquid, and in various forms including microspheres, powders, emulsions and the like. The carrier may be one or more of a number of carriers that impart a variety of properties, such as greater stability, wettability or dispersibility. Wetting agents, such as natural or synthetic surfactants, which can be nonionic or ionic surfactants, or a combination thereof, can be included in a composition of the invention. Water-in-oil emulsions can also be used to formulate a composition that includes the purified population (see, for example, US Patent No. 7,485,451). Suitable formulations that can be prepared include wettable powders, granules, gels, agar strips or pellets, thickeners, biopolymers and the like, microencapsulated squid and the like, | liquids such as aqueous fluids, aqueous suspensions, oil-in-water emulsions, etc. The formulation may include grain or legume products, for example, ground grains or beans, broths or flour derived from grains or beans, starch, sugar or oil.
[00862] [00862] In some embodiments, the agricultural carrier may be soil or a plant growth medium. Other agr-ball carriers that can be used include water, fertilizers, herbal oils, humectants or combinations of these. Alternatively, the agricultural ball carrier may be a solid, such as diatomaceous earth, clay, silica, alginate, clay, bentonite, vermiculite, seed pods, other plant and animal products, or combinations, including granules, pellets or suspensions. Mixtures of any of the aforementioned ingredients are also contemplated as carriers, such as, but not limited to pesta (flour and kaolin clay), agar or pallets based on clay, sand or clay etc. The formulations may include food sources for cultivated organisms, such as barley, rice, wheat or other biological materials, such as seeds, plant elements, sugarcane bagasse, grain processing bark or stems, ground vegetable material or wood from the tailings of a work, sawdust or small fibers for recycling paper, fabric or wood. Other suitable formulations will be known to those skilled in the art.
[00863] [00863] In one embodiment, the formulation may include a tackifier or adherent. Such agents are useful for combining the complex population of the invention with carriers that may contain other compounds (e.g., non-biological control agents), to yield a coating composition. Such compositions help to create coatings around the plant or plant element to maintain contact between the endophyte and other agents with the plant or plant element. In some modalities, the adherents are selected from the group consisting of: alginate, gums, starches, lecithins, formononetine, polyvinyl alcohol, alkaline formononetinate, hesperetin, polyvinyl acetate, cephalins, arabic gum, xanthan gum, carrageenan, PGA, others biopolymers, Mineral Oil, Polyethylene Glycol (PEG), Polyvinylpyrrolidone (PVP), Arabino-galactan, Methylcellulose, PEG 400, Chitosan, Polyacrylamide, Polyacrylate, Polyacrylonitrile, Glycerol, Triethylene Glycol, Polyvinyl Acetate, Carboxylic Acid, Glycol, Gelatin, Polyethylene Glycol, Carboxylic, Glycol , Ghatti gum and polyoxyethylene-polyoxybutylene block copolymers. Other examples of adherent compositions that can be used in the synthetic preparation include those described in EP 0818135, CA 1229497, WO 2013090628, EP 0192342, WO 2008103422 and CA 1041788.
[00864] [00864] It is also contemplated that the formulation may also comprise an anti-caking agent.
[00865] [00865] The formulation can also contain a surfactant, humectant, emulsifier, stabilizer or defoamer. Non-limiting examples of surfactants include nitrogen-surfactant mixtures such as Prefer 28 (Cenex), Surf-N (USA), Inhance (Brandt), P-28 (Wilfarm) and Patrol (Helena); esterified seed oils include Sun-lt 1l (AMCy), MSO (UAP), Scoil (Agsco), Hasten (Wilfarm) and Mes-100 (Drexel); and organo-silicone surfactants include Silwet L77 (UAP), Silicine (Terra), Dyne-Amic (Helena), Cinica (Helena), Sylgard 309 (Wilbur-Ellis) and Century (Precision), polysorbate 20, polysorbate 80, Tween 20, Tween 80, Scattics, Alktest TW20, Canarcel, Peogabsorb 80, Triton X-100, Conco NI, Dowfax 9N, Igebapl CO, Makon, Neutronyx 600, Nonipol NO, Plytergent B, Renex 600, Solar NO, Sterox, Serfonic N , T-DET-N, Tergitol NP, Triton N, IGEPAL
[00866] [00866] In certain cases, the formulation includes a microbial stabilizer. Such an agent can include a desiccant. As used herein, a "desiccant" can include any compound or mixture of compounds that can be classified as desiccant, regardless of whether the compound or compounds are used in concentrations that actually have a drying effect on the inoculant | liquid. Such desiccants are ideally compatible with the population used and should promote the ability of the endophytic population to survive application to seeds and survive desiccation. Examples of suitable desiccants include one or more of trehalose, sucrose, glycerol and methylene glycol. Other suitable desiccants include, but are not limited to, non-reducing sugars and sugar alcohols (for example, mannitol or sorbitol). The amount of desiccant introduced into the formulation can vary from about 5% to about 50% by weight / volume, for example, between about 10% to about 40%, between about 15% and about 35%, or between about 20% and about 30%. In some embodiments, components of a sugar-based microbial stabilizer include, but are not limited to, glucose, sucrose, polyvinylpyrrolidone K 30 (PVP30K), mineral oil, soy lecithin, peptone, monopotassium phosphate (KH2PO4) and dipotassium phosphate (K2HPO4). In an alternative embodiment, the components of a sugar-free microbial stabilizer include, but are not limited to, polyvinylpyrrolidone K 30 (PVP30K), polyvinylpyrrolidone / vinyl acetate (PVP-VA), soy lecithin, peptone, mineral oil, hydroxypropyl guar (HP-Guar), “monopotassium phosphate (KH2PO4) and dipotassium phosphate (K2HPO4). The components of exemplary microbial stabilizers for use with the invention described in this document are shown in Table 1 and Table 2.
[00867] [00867] In some cases, it is advantageous that the formulation contains agents such as a fungicide, anti-complex agent, herbicide, nematicide, insecticide, plant growth regulator, rodenticide, bactericide, virucide or nutrient . Such agents are ideally compatible with the element of the agr Cola plant or the seedling on which the formulation is applied (for example, it should not be harmful to the growth or health of the plant). In addition, the agent is ideally one that does not cause safety concerns for human, animal or industrial use (for example, without safety problems, or the compound is sufficiently | sufficient for the plant-derived plant product to contain negligible amounts of compound).
[00868] [00868] In the liquid form, for example, solutions or suspensions, the endophytic populations can be mixed or suspended in water or in aqueous solutions. Thinners or carriers | Suitable liquids include water, aqueous solutions, petroleum distillates or other carriers | liquid.
[00869] [00869] Solid compositions can be prepared by dispersing the endophyte populations of the invention in a suitably divided solid carrier, such as peat, wheat, bran, vermiculite, clay, talc, bentonite, diatomaceous earth, fuller soil, pasteurized soil and the like. When these formulations are used as wettable powders, biologically compatible dispersing agents, such as nonionic, anionic, amphoteric or cationic emulsifying agents and dispersants, can be used.
[00870] [00870] In some cases, a fluidity polymer, also known as plantability polymer, such as Flo Riteº, for example, Flo-Riteº 1706 (BASF, Ludwigshafen, Germany). In some embodiments, a number of fluidity or plantability is DISCOTY AG (Incotec, Enkhuizen, Pa 6 & the Netherlands). In some embodiments, a polymer of fluidity or plantability is KannarO Universal Wonder (Kannar Earth Science, Ltd., Buford, GA).
[00871] [00871] The solid carriers used in the formulation include, for example, mineral carriers, such as kaolin clay, pyrophyllite, bentonite, montmorillonite, diatomaceous earth, acidic white soil, vermiculite and perlite and inorganic salts such as ammonium sulfate, phosphate ammonium, ammonium nitrate, urea, ammonium chloride and calcium carbonate. In addition, fine organic powders, such as wheat flour, wheat bran and rice bran, can be used. The carriers | liquids include vegetable oils such as soybean oil, neem oil, cottonseed oil and other compositions such as glycerol, ethylene glycol, polyethylene glycol, propylene glycol, polypropylene glycol! etc.
[00872] [00872] In one embodiment, the formulation is ideally suited for coating a population of endophytes in plant elements. The endophyte populations described in the present invention are able to confer many suitability benefits on host plants. The ability to confer such benefits by coating populations on the surface of plant elements has many potential advantages, particularly when used on a commercial scale (agr Cola).
[00873] [00873] The endophyte populations in this document can be combined with one or more of the agents described above to produce a formulation suitable for combination with an element of the agricultural plant, seedling or other plant element. Endophyte populations can be obtained from growing in culture, for example, using a synthetic growth medium. In addition, endophytes can be grown in a solid medium, for example in Petri dishes, scraped and suspended in preparation. Endophytes at different growth stages can be used. For example, endophytes in delayed phase, initial log phase, half log phase, final log phase, stationary phase, early death phase or death phase can be used. Endophytic spores can be used for the present invention, for example, but not limited to: arthropospores, sporangiospores, coniliums, clamadospores, pycnidiospores, endospores, zoospores.
[00874] [00874] Formulations comprising endophyte populations typically contain between about 0.1 and 95% by weight, for example, between about 1% and 90%, between about 3% and 75%, between about 5% and 60%, between about 10% and 50% in wet weight of the population. It is preferable that the formulation contains at least about 10º3 CFU per ml of formulation, for example, at least about 10% 4, at least about 105, at least about 106, at least about 1097 CFU, at least about 10º8 CFU per ml! formulation. It is preferable that the formulation is applied to the plant element at about 10º2 CFU / seed, between 102 and 103 CFU, at least about 10º3 CFU, between 10º3 and 104 CFU, at least about 104 CFU, between 1094 and 10º5 CFU, at least about 10º5 CFU, between 10º5 and 106 CFU, at least about 106 CFU, between 10% 6 and 10º7 CFU, at least about 10º7 CFU, between 107 and 108 CFU, or even more than 10º8 CFU per seed.
[00875] [00875] In another embodiment, the invention provides a substantially uniform population of synthetic compositions comprising plant elements (PEs), comprising two or more PEs comprising the endophytic population, as described in this document, above. Substantial uniformity can be determined in several ways. In some cases, at least 10%, between 10% and 20%, for example, at least 20%, between 20% and 30%, at least 30%, between 30% and 40%, at least 40%, between 40 % and 50%, at least 50%, between 50% and 60%, at least 60%, between 60% and 70%, at least 70%, between 70% and 75%, at least 75%, between 75% and 80%, at least 80%, between 80% and 90%, at least 90%, between 90% and 95%, at least 95% or more of the PEs in the population, comprise the endophytic population in an amount effective to colonize a plant or plants derived from said PEs when arranged on the surface of the PEs. In other cases, at least 10%, between 10% and 20%, for example, at least 20%, between 20% and 30%, at least 30%, between 30% and 40%, at least 40%, between 40 % and 50%, at least 50%, between 50% and 60%, at least 60%, between 60% and 70%, at least 70%, between 70% and 75%, at least 75%, between 75% and 80%, at least 80%, between 80% and 90%, at least 90%, between 90% and 95%, at least 95% or more of the plant elements of the population, contain at least 1, between 10 and 10, 10, between 10 and 100, or 100 CFU on the surface of the plant element or per gram of plant element, for example, between 100 and 200 CFU, at least 200 CFU, between 200 and 300 CFU, at least 300 CFU, between 300 and 1,000 CFU, at least 1,000 CFU, between 1,000 and 3,000 CFU, at least 3,000 CFU, between 3,000 and 10,000 CFU, at least 10,000 CFU, between 10,000 and 30,000 CFU, at least 30,000 CFU, between 30,000 and 100,000 CFU, at least 100,000 CFU, between 100,000 and 300,000 CFU, at least 300,000 CFU, between 300,000 and
[00876] [00876] In a particular embodiment, the population of the plant elements is packed in a bag or container suitable for commercial sale. This bag contains a unit weight or count of the elements of the plant comprising the endophytic population as described in this document and further comprising a label. In one embodiment, the bag or container contains at least 100 plant elements, between 100 and 1,000 plant elements, 1,000 plant elements, between 1,000 and 5,000 plant elements, for example, at least 5,000 plant elements, between 5,000 and 10,000 plant elements, at least 10,000 plant elements, between 10,000 and 20,000 plant elements, at least 20,000 plant elements, between 20,000 and
[00877] [00877] In some cases, a subpopulation of seeds comprising the endophytic population is still selected based on the increase in uniformity, for example, based on the uniformity of the microbial population. For example, elements of individual plants from groups collected from individual ears, individual plants, individual land plots (representing plants inoculated on the same day) or individual fields can be tested for uniformity of microbial density, and only those groups that meet specifications (for example, at least 80% of the plant elements tested have minimal density, as determined by the quantitative methods described elsewhere) are combined to provide the sub-population of agr Colas seeds.
[00878] [00878] The methods described in this document can also comprise a validation step. The validation step may involve, for example, the growth of some elements of the plant collected from the inoculated plants in mature glue agr plants, and testing these individual plants for uniformity. This validation step can be performed on individual seeds collected from ears, individual plants, individual plots of land (representing plants inoculated on the same day) or individual fields, and tested as described above to identify groups that meet the required specifications.
[00879] [00879] In some embodiments, the methods described in this document include planting a synthetic composition described in this document. Suitable planters include an air fertilizer and / or seeder used in agricultural operations to apply particulate materials including one or more of the following, seeds, fertilizers and / or inoculants to the soil during the planting operation. The fertilizer / seeder can include a bar of tools that have openers for coupling to the ground, behind which a wheeled cart is towed that includes one or more containment tanks or containers and associated measuring means to, respectively, contain and measure particulate materials.
[00880] [00880] In certain embodiments, a composition described in this document can be in the form of a | Guido, a suspension, a solid or a powder (wettable powder or dry powder). In another embodiment, a composition can be in the form of a seed coating. State compositions | liquid, suspension or powder (eg wettable powder) form may be suitable for coating plant elements. When used to coat plant elements, the composition can be applied to the plant elements and left to dry. In embodiments where the composition is a powder (for example, a collectable powder), it may be necessary to add a | fluid, such as water, to powder, before application to a seed.
[00881] [00881] In yet another embodiment, the methods may include introducing into the soil an inoculum from one or more of the endophyte populations described in this document. Such methods may include introducing into the soil one or more of the compositions described in this document. The inoculum (s) or compositions can be introduced into the soil according to methods known to those skilled in the art. Non-limiting examples include introduction into the furrow, spraying, seed coating, leaf introduction, etc. In a specific embodiment, the introduction step comprises the introduction into the groove of the inoculum or compositions described in this document.
[00882] [00882] In one embodiment, the elements of the plant can be treated with the composition (s) described in this document in several ways, but preferably by spraying or dripping. Spray and drip treatment can be conducted by formulating compositions described in this document and spraying or dripping the composition (s) onto (one) seed (s) using a continuous treatment system (which is calibrated to apply treatment to a predefined rate in proportion to the continuous flow of seed), such as a type of attendant drum. Group systems, in which a predetermined lot size of seeds and composition (s), as described in this document, are delivered in a mixer, can also be employed.
[00883] [00883] In another modality, the treatment involves the coating of plant elements. One of these processes involves coating the inside wall of a round container with the composition (s) described in this document, adding plant elements and then rotating the container to make the plant elements come into contact with the wall and the composition (s), a process known in the art as "container coating". Plant elements can be coated by combinations of coating methods. Immersion typically involves the use of fluid forms of the described compositions. For example, plant elements can be soaked for about 1 minute to about 24 hours (for example, for at least 1 min, between 1 and 5 min, 5 min, between 5 and 10 min, 10 min, between 10 and 20 min, min, between 20 and 40 min, 40 min, between 40 and 80 min, 80 min, between 80 min and 3h, 3 h, between 3 am and 6 am, 6 am, between 6 am and 12 pm, 12 pm, between 12 pm and 24h, 24h).
[00884] [00884] One of the main focuses of efforts to improve crops has been to select varieties with traits that provide, in addition to greater return, greater homogeneity and uniformity. If on the one hand inbreeding can produce plants with substantial genetic identity, heterogeneity in relation to plant height, flowering time and seed time, there are impediments that remain to obtain a homogeneous field of plants. The inevitable plant-to-plant variability is caused by a number of factors, including environmental conditions and irregular management practices. Another possible source of variability may, in some cases, be due to the heterogeneity of the endophytic population that inhabits the plants. By providing endophytic populations to the plant's reproductive elements, the resulting plants generated by the germination of the plant's reproductive elements have a more consistent collection of endophytes and are therefore expected to produce a more uniform plant population.
[00885] [00885] Therefore, in another embodiment, the invention provides a substantially uniform population of plants. The population can include at least 5 plants, between 5 and 10 plants, at least 10 plants, between 10 and 100 plants, for example, at least 100 plants, between 100 and 300 plants, at least 300 plants, between 300 and 1,000 plants at least 1,000 plants, between 1,000 and
[00886] [00886] The uniformity of plants can be measured in several different ways. In some embodiments, there is an increased uniformity with respect to endophytes within the plant population. For example, in some embodiments, a substantial portion of the plant population, for example at least 10%, between 10% and 20%, at least 20%, between 20% and 30%, at least 30%, between 30% and 40%, at least 40%, between 40% and 50%, at least 50%, between 50% and 60%, at least 60%, between 60% and 70%, at least 70%, between 70% and 75% at least 75%, between 75% and 80%, at least 80%, between 80% and 90%, at least 90%, between 90% and 95%, at least 95% or more of the elements or plants in a population contain a limit number of an endophyte population. The limit number can be at least 10 CFU, between 10 and 100 CFU, at least 100 CFU, between 100 and 300 CFU, for example, at least 300 CFU, between 300 and 1,000 CFU, at least 1,000 CFU, between 1,000 and 3,000 CFU, at least 3,000 CFU, between 3,000 and 10,000 CFU, at least 10,000 CFU, between 10,000 and 30,000 CFU, at least 30,000 CFU, between 30,000 and 100,000 CFU, at least 100,000 CFU or more, at the plant or part of the plant . Alternatively, in a substantial portion of the plant population, for example, I push at least 1%, between 1% and 10%, at least 10%, between 10% and 20%, at least 20%, between 20% and 30%, at least 30%, between 30% and 40%, at least 40%, between 40% and 50%, at least 50%, between 50% and 60%, at least 60%, between 60% and 70%, at least 70%, between 70% and 75%, at least 75%, between 75% and 80%, at least 80%, between 80% and 90%, at least 90%, between 90% and 95%, at least 95% or more of the plants in the population, the endophytic population that is supplied to the seed or seedling represents at least 0.1%, between 0.1% and 1% at least 1%, between 1% and 5%, at least
[00887] [00887] In one embodiment, there is increased genetic uniformity of a substantial proportion or of all detectable endophytes within the rate, genus or species of a component relative to an uninoculated control. This increased uniformity may be a result of the endophyte being of monoclonal origin or derived from a population that comprises a more uniform sequence of the genome and plasmid repertoire than that which would be present in the population of endophytes a plant that derives its endophytic community mainly through assimilation of different soil symbionts.
[00888] [00888] In another modality, there is an increased uniformity in relation to a physiological parameter of plants within the population. In some cases, there may be an increased uniformity in plant height when compared to a population of reference agricultural plants grown under the same conditions. For example, there may be a reduction in standard deviation in plant height in the population of at least 5%, between 5% and 10%, for example, at least 10%, between 10% and 15%, minus 15%, between 15 % and 20%, at least 20%, between 20% and 30%, at least 30%, between 30% and 40%, at least 40%, between 40% and 50%, at least 50%, between 50% and 60%, at least 60% or more, when compared to a population of agricultural reference glues grown under the same conditions. In other cases, there may be a reduction in the standard deviation in the flowering time of the plants in the population of at least 5%, between 5% and 10%, for example, at least 10%, between 10% and 15%, at least 15 %, between 15% and 20%, at least 20%, between 20% and 30%, at least 30%, between 30% and 40%, at least 40%, between 40% and 50%, at least 50% , between 50% and 60%, at least 60% or more, when compared to a population of agricultural reference glues grown under the same conditions.
[00889] [00889] The present invention provides a plant commodity product, as well as methods for producing a plant commodity product, which is derived from a plant. As used in this document, a "plant commodity product" refers to any composition or product that consists of material derived from a plant, seed, plant cell or plant element of the present invention. Commodity plant products can be sold to consumers and can be viable or non-viable. Non-viable commodity products include, but are not limited to, plant elements and unviable grains; processed seeds, seed parts and plant elements; dehydrated vegetable tissue, frozen vegetable tissue and processed vegetable tissue; seeds and plant elements processed for animal feed for terrestrial and / or aquatic consumption of animals, oil, bran, flour, flakes, bran, fibers, paper, tea, coffee, silage, crushed whole grains and any other food for human use or animal consumption, such as fruit or another edible portion of the plant; and biomass and fuel; and raw material in the industry.
[00890] [00890] The industrial uses of oils derived from agricultural plants described in this document include ingredients for paints, plastics, fibers, detergents, cosmetics, lubricants and biodiesel fuel. Vegetable oils can be separated, interesterified, sulfurized, epoxidized, polymerized, ethoxylated or cleaved. Designing and producing vegetable oil derivatives with enhanced functionality and enhanced oleochemistry is a rapidly growing field. For example, a mixture of triglycerides is usually divided and separated into pure fatty acids, which are then combined with alcohols or acids derived from petroleum, nitrogen, sulfonates, chlorine or with fatty alcohols derived from fats and oils to produce the desired type of oil or fat. Commodity plant products also include industrial compounds, such as a wide variety of resins used in the formulation of adhesives, by squid, plastics, paints, coatings and foams.
[00891] [00891] As described in this document, the populations of purified endophytes and synthetic compositions that comprise them (for example, formulations) can be used to confer beneficial traits to the host plant.
[00892] [00892] The invention will be further described in the following examples, which do not limit the scope of the invention described in the claims. Each patent application, magazine article, citation and other references are incorporated into this document by reference in its entirety, as if each had been incorporated by reference individually.
[00893] [00893] The isolation and cultivation of endophytic microbes from agricultural plants was carried out using methods well known in the art. MIC-15870 was isolated from the breeds of the domesticated B73 strain of Zea mays. MIC-84414 was isolated from seeds of Bouteloua gracilis. MIC-68000 was isolated from the seeds of a Zea mays tica from the southwestern United States. DNA was extracted from the tissues using the DNeasy DNA extraction kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. The endophytes were characterized by the sequences of genomic regions, these sequences are listed in Table 4. The primers that amplify the genomic regions of the endophytes of the present invention are listed in Table 3. The additional isolation and cultivation of endophytic microbes from agricultural plants Colas was performed using methods well known in the art. MIC-99023 was isolated from Platycladus orientalis tissue. MIC-19994 was isolated from Platycladus orientalis tissue. These endophytes were characterized by the sequences of genomic regions, these sequences are listed in Table 4. The primers that amplify the genomic regions of the endophytes of the present invention are listed in Table 3. Additional isolation and cultivation of endophytic microbes from agricultural plants was carried out using methods well known in the art. MIC-82330 was isolated from the seeds of a rye cultivar. Endophytes were characterized by the sequences of genomic regions, these sequences are listed in the Table
[00894] [00894] MIC-15870 was deposited with the Agricultural Research Service Culture Collection (NRRL), United States Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604, under the terms of the Budapest Treaty, as Deposit ID: NRRL-67466.
[00895] [00895] MIC-84414 was deposited with the Agricultural Research Service Culture Collection (NRRL), United States Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604, under the terms of the Budapest Treaty, as Deposit ID: NRRL-67467.
[00896] [00896] MIC-82330 was deposited with the Agricultural Research Service Culture Collection (NRRL), United States Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604, under the terms of the Budapest Treaty, as Deposit ID: NRRL-B67465.
[00897] [00897] The bacterial endophytes described in this document were further characterized by the sequence of factor G of elongation of the marker gene. PCR amplification of the stretching factor G gene using primer sequences fusA-f2 (SEQ ID NO: 30) and fusA-r2 (SEQ ID NO: 31) is described in Miyoshi-Akiyama T, Hayakawa K, Ohmagari N, Shimojima M, Kirikae T (2013) Multilocus Sequence Typing (MLST) for Characterization of Enterobacter cloacae. PLoS ONE 8 (6): e66358. doi: 10.1371 / journal.pone.0066358. The sequence of the stretching factor G in MIC-82330 is SEQ ID NO: 49.
[00898] [00898] The endophytes described in this document can be identified by the sequence of one or more of the following loci: long subunit rRNA (LSU), small subunit rRNA (SSU), second largest RNA polymerase Il (RPB2) subunit, beta- tubulin, ribosomal protein 60S L 10. PCR amplification of LSU using primer sequences LROR (SEQ ID NO: 26 and SEQ ID NO: 26) and LR5 (SEQ ID NO: 27 and SEQ ID NO: 27) is described in Stielow JB, Lévesque CA, Seifert KA, et al. One fungus, which genes Development and assessment of universal primers for potential secondary fungal DNA barcodes. Persoonia: Molecular Phylogeny and Evolution of Fungi. 2015; 35: 242-
[00899] [00899] MIC-84414 can be identified by the sequence of one or more of the following: RPB2 sequence (SEQ ID NO: 35), RPB2 sequence (SEQ ID NO: 36), beta-tubulin sequence (SEQ ID NO: 37). MIC-19994 can be identified by the sequence of one or more of the following: its LSU sequence (SEQ ID NO: 41), SSU sequence (SEQ ID NO: 42). MIC-99023 can be identified by the sequence of one or more of the following: the gene encoding the 60S L10 ribosomal protein (SEQ ID NO: 46), the RPB2 sequence (SEQ ID NO: 47), the beta sequence -tubulin (SEQ ID NO: 48).
[00900] [00900] The classification of bacterial strains using their 16S sequence was made using the following methodology.
[00901] [00901] To accurately characterize isolated bacterial endophytes, colonies were subjected to marker gene sequencing, and the sequences were analyzed to provide taxonomic classifications. The colonies were submitted to PCR amplification of the 168 rRNA gene using a primer pair 27f (S-AGAGTTTGATYMTGGCTCAG-3 ') (SEQ ID NO: 1) and 1492r (S-GGTTACCTTGTTACGACTT-3') (SEQ ID NO: 2 ). The sequencing reactions were performed using primers: 27f (5 - AGAGTTTGATYMTGGCTCAG-3 ') (SEQ I | D NO 1) 515f (& - GTGYCAGCMGCCGCGGTAA-3') (SEQ I | D NO 3) 806 (58 - GGACTACNVGGGTTTCAT ') (SEQ | D NO: 4) and 1492r (5 - GGTTACCTTGTTACGACTT-3') (SEQ ID NO: 2). Preferably, sequencing primers are chosen so that overlapping regions are sequenced. Sanger sequencing was performed in Genewiz (South Plainfield, NJ). The raw chromatograms were converted to sequences, and the corresponding quality scores were assigned using TraceTuner v3.0.6beta (US 6,681,186). These strings were filtered for quality, and a consensus string was generated using Geneious v
[00902] [00902] Taxonomic classifications were assigned to strings using the highest probability of attribution based on the results of industry standard taxonomic classification tools: LCA (uses USEARCH (Edgar, RC, 2010) with global search option and then for all best hits, returns lowest taxonomic rating shared by all best hits for one query), RDP Naive Bayesian rRNA Classifier version
[00903] [00903] The classification of the fungal strain using ITS sequences was made by the following methodology.
[00904] [00904] Total genomic DNA was extracted from individual fungi isolates using the DNeasy Plant Mini Kit (Qiagen, Germantown, MD). Polymerase Chain Reaction (PCR) was used to amplify a genomic region that includes nuclear ribosomal internal transcript spacers (ITS) using an ITS 1 (S-CTTGGTCATTTAGAGGAAGTAA-3 ') primer pair (SEQ ID NO: 5) and LR5 (5-TCCTGAGGGAAACTTCG-3 ') (SEQ ID NO: 8). Each microliter reaction mix includes 22.5 microliters of the Invitrogen Platinum Tag supermix, 0.5 microliters of each primer (10 µM) and 1.5 microliters of DNA template (-2-4 ng). The cyclization reactions were carried out with PTC thermal cyclers from MJ Research and consisted of 94 “C for 5 min, 35 cycles of 94“ C for 30 s, 54 “C for s and 72ºC for 1 min, and 72ºC for 10 min. Sanger sequencing was performed in Genewiz (South Plainfield, NJ) using primers: ITS 1 (5'- CTTGGTCATTTAGAGGAAGTAA-3 ') (SEQ I | D NO 5), | TS2 (5- GCTGCGTTCTTCATCGATGC-3') (SEQ I | D NO 6) TS 3 (5- GCATCGATGAAGAACGCAGC-3 ') (SEQ | D NO 7) and LR5 (5- TCCTGAGGGAAACTTCG-3') (SEQ ID NO: 8). Sequencing primers were chosen so that the overlapping regions were sequenced. The raw chromatograms were converted to sequences, and the corresponding quality scores were assigned using TraceTuner v3.0.6beta (US
[00905] [00905] Taxonomic classifications were assigned to strings using the highest probability of attribution based on the results of industry standard taxonomic classification tools: LCA (uses USEARCH (Edgar, RC, 2010) with global search option and then to all the best hits, returns the lowest taxonomic classification shared by all the best hits for a query), SPINGO (Allard et al., 2015), and UTAX (Edgar, RC, 2016), using the WARCUP Fungal ITS trainset 1 ( Deshpande et al. (2016)
[00906] [00906] Seed preparation: The quality of the soybean seed lot was evaluated by testing the germination of 100 seeds. The seeds were placed, 8 seeds per Petri dish, on filter paper in Petri dishes, 12 ml of water were added to each dish and the dishes incubated for 3 days at 24ºC. The germination percentage was greater than 95%. A thousand soybean seeds were then sterilized on the surface by coincubation with chlorine gas in a 20 x 30 cm container placed in a chemical hood for 16 hours. The germination percentage of 50 seeds, per sterilization batch, was tested as above and confirmed as greater than 95%.
[00907] [00907] Preparation and heterologous disposition of endophytes: The spore solutions were made by rinsing and scraping the spores of the inclined agar that have been growing for about 1 month. The washing was done with 0.05% Silwet. Solutions were passed through Miracloth to filter mycelia. Spores per ml were counted under a microscope using a hemocytometer. The stock suspension was then diluted to 106 spores / ml using water. 3 yàl of spore suspensions per seed were used (-10º3 CFUs / seed are obtained). Control treatments were prepared by adding equivalent volumes of sterile water to the seeds.
[00908] [00908] Seedling vigor test: Two rolled pieces of germination paper were placed in a sterile glass jar with 50 mL of sterile water and then removed when completely saturated. Then the papers were separated and the inoculated seeds were placed at intervals of approximately 1 cm along the length of a sheet of moist germinated paper, at least 2.5 cm from the top of the paper and 3.8 cm from the margin of the paper. The second sheet was placed on top of the soybean seeds and the paper and layered seeds were rolled without precision in a tube shape. Each tube was secured with a rubber band around its middle and placed in a single sterile glass bottle and covered with a lid. For each treatment, three bottles with 15 seeds per bottle were prepared. The position of the vials with the growth chamber was randomized. The flasks were incubated at 60%
[00909] [00909] Dirt, excess water, seeds and other debris were removed from the seedlings to allow an accurate sweeping of the dogs. The individual seedlings were arranged in transparent plastic trays and the trays were arranged on an Epson Expression 11000XL scanner (Epson America, Inc., Long Beach CA). The roots were arranged manually to reduce the amount of overlap. For the measurements of the rafes, the shoots were removed if the shape of the bud caused it to overlap the ra bes.
[00910] [00910] The WinRHIZO Arabidopsis Pro2016a software version (Regents Instruments, Quebec, Canada) was used with the following acquisition settings: 4000 dpi grayscale image, "speed priority", "overlapping" ( overlap) (1 object), Root Morphology ("Root Morphology"): Precision ("Precision") (default), Crossing Detection ("Crossover Detection") (normal). The scanning area was defined as the maximum area of the scanner. When the scan was completed, the root area was selected and the root length and root surface area were measured.
[00911] [00911] The statistical analysis was performed using R (R Core Team, 2016. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. R-project.org/).
[00912] [00912] The endophytic treatment comprising MIC-15870 resulted in a 23% increase in the average length of treated soybean seedlings and a 40.5% increase in the average area of treated soybean seedlings compared to to untreated soy seedlings. Treatment with endophytes comprising MIC-84414 resulted in an 11% increase in the average length of the treated soybean seedlings and a 19% increase in the average area of the treated soybean seedlings in relation to non-soybean seedlings. treated. The results are summarized in Table 8.
[00913] [00913] Seed preparation: The quality of the corn seed lot is evaluated first for germination by transferring 100 seeds and with 3.5 ml of water to a Petri dish coated with filter paper. The seeds are incubated for 3 days at 24ºC and to ensure that the germination percentage is greater than 95%. A thousand corn seeds are then sterilized on the surface by coincubation with chlorine gas in a 20 x 30 cm container in a chemical extractor for 12 hours. The germination percentage of 50 seeds, per sterilization batch, is tested as above and confirmed as greater than 95%.
[00914] [00914] Optional reagent preparation: 7.5% PEG 6000 (Calbiochem, San Diego, CA) is prepared by adding 75 g of PEG to 1000 mL of water and then stirred on a hot plate until PEG be completely dissolved. The solution is then autoclaved.
[00915] [00915] Preparation and heterologous disposition of endophytes: Spore solutions are made by rinsing and scraping the slant agar spores that have been growing for about 1 month. Washing is done with 0.05% Silwet. Solutions are passed through Miracloth to filter mycelia. Spores per ml are counted under a microscope using a hemocytometer. The stock suspension is then diluted to 1056 spores / ml using water. 3 ul of spore suspensions are used per seed (-10º3 CFUs / seed are obtained). Control treatments are prepared by adding equivalent volumes of sterile water to the seeds.
[00916] [00916] Seedling vigor test: 25 ml of sterile water (or, optionally, 25 ml of PEG solution, as prepared above) are added to each Cyg'Y germination bag (Mega International, Newport, MN)
[00917] [00917] Statistical analysis is performed using R (R Core Team, 2016. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. R-project.org/).
[00918] [00918] Seed preparation: The batch of wheat seeds was evaluated for the first time for germination by transferring 100 seeds and with 8 mL of water to a Petri dish coated with filter paper. The seeds were incubated for 3 days at 24ºC and the germination percentage was higher than 95%. The wheat seeds were then sterilized on the surface by coincubation with chlorine gas in a 20 x 30 cm container in a chemical extractor for 12 hours. The germination percentage of 50 seeds, per sterilization batch, was tested as above and confirmed as above 95%.
[00919] [00919] Reagent preparation: 7.5% polyethylene glycol was prepared! (PEG) by adding 75 g of PEG to 1000 ml of water and then stirring them on a hot plate in a warm state until the PEG is completely dissolved. The solution was then autoclaved.
[00920] [00920] Preparation and heterologous disposition of endophytes: The spore solutions were made by rinsing and scraping the spores of the inclined agar that had been growing for about 1 month. The washing was done with 0.05% Silwet. Solutions were passed through Miracloth to filter mycelia. Spores per ml were counted under a microscope using a hemocytometer. The stock suspension was then diluted to 106 spores / ml using water. 3 ul of spore suspensions per seed were used (-10º3 CFUs / seed were obtained). Seeds and spores were combined with a 50 mL falcon tube and shaken gently for 5 to 10 seconds until completely coated. Control treatments were prepared by adding equivalent volumes of sterile water to the seeds.
[00921] [00921] Seedling vigor test: Petri dishes were prepared by adding four sheets of heavy sterile seed germination paper, then adding 50 mL of PEG solution as prepared above to each plate, allowing the | liquid completely immersed in all the leaves. The sheets were positioned and then folded so that the back of the board and a side wall were covered, two sheets were then removed and placed on a sterile surface. Along the edge of the plate in front of the covered side wall, 15 inoculated wheat seeds were placed evenly at least an inch from the top of the plate and half an inch from the sides. The seeds were placed with the smooth side up and with the tip pointed at the side wall of the plate covered with germination paper. The seeds were covered by the two reserved leaves, and the moist layers of paper were smoothed to remove air bubbles and trap the seeds, and the cover was replaced. For each treatment, at least three plates with 15 seeds per plate were prepared. The plates were then randomly distributed in piles of 8 to 12 plates and a seedless plate was placed on top. The cells were incubated at 60% relative humidity and 22ºC day, 18ºC night with 12 hours of light and 12 hours of darkness for 24 hours, then each plate was turned to a semi-vertical position with the side wall covered with paper at the bottom. The plates were incubated for another 5 days, then the wheat seeds were manually marked for germination, sprout length and root.
[00922] [00922] Statistical analysis was performed using R (R Core Team, 2016. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. R-project.org/).
[00923] [00923] The endophilic treatment comprising MIC-19994 resulted in a 13% increase in the average length of the roots of the treated wheat seedlings. The endophytic treatment comprising MIC-84414 resulted in a 40% increase in the average length of the treated wheat seedlings. The results are shown in Table 9.
[00924] [00924] MIC-84414 and MIC-19994 were grown for 5-7 days in culture | liquid using molasses yeast extract (MYE) broth. The biomass of these cultures was filtered to remove the spent juice, and the resulting fungal material was air-dried and ground through a 40 µm mesh.
[00925] [00925] MIC-84414 and MIC-19994 were produced by solid state fermentation and subsequent spore preparations to remove the growth substrate.
[00926] [00926] The biomass for the MIC-15870 was produced by cultivation in MYE broth for 5-7 days. The biomass of the culture was filtered to remove spent broth, and the resulting fungal material was air dried and ground through a 40 µm mesh.
[00927] [00927] The biomass for MIC-99023 was produced by growing in medium | liquid (PDB). The resulting biomass was homogenized by sonication (50% amplitude for 30 seconds) or in a FastPrep-24 (MP Biomedicals, Santa Ana, CA, USA) set at 4.5 m / s for 30 seconds.
[00928] [00928] A 2% wt / volume solution of low viscosity methylcellulose (LVMC) for seed coatings was prepared by the following method. 20 ml are used! of the LVMC solution for each kg of seed. An Erlenmeyer flask was filled with the appropriate volume of deionized water and heated to 50 degrees Celsius on a hot plate with stirring using a stir bar. The appropriate mass of LVMC powder for the desired final concentration solution was slowly added until it dissolved. The solution was autoclaved at 121 degrees Celsius at 15 PSI for 30 minutes to sterilize. Finally, the sterilized LVMC was placed on a rotary shaker (130 rom) at room temperature for several hours to promote reliquification of the solution.
[00929] [00929] The talcum powder for powdered seed coatings was prepared by the following method. The talcum powder was aliquoted in 2.8 L fernbach bottles and autoclaved in a dry cycle (121 degrees Celsius at 15 PSI for 30 minutes) to sterilize.
[00930] [00930] For dry formulated strains, prior to seed treatment, equal parts of the powdered endophyte biomass prepared in Example 5 and sterile talcum powder were mixed. Control treatments are prepared using equivalent volumes of sterile talcum powder.
[00931] [00931] The seeds treated with dry formulation endophytes were prepared by first applying 2% LVMC (16.6 ml! Per kg of seed) to the seeds and shaking them to disperse the sodium alginate evenly in the seeds. Then, the endophyte / talcum powder mixture was added and the seeds were agitated to disperse the powder evenly in the seeds. Then, Flo-Riteº 1706 (BASF, Ludwigshafen, Germany) (2.0 oz / cwt of seeds) was added and the seeds were agitated to disperse the polymer evenly in the seeds.
[00932] [00932] For strains formulated with | liquid, before seed treatment, equal parts of biomass | liquid and 2% LVMC were combined. The LVMC / biomass inoculum was added to the seeds and agitated to uniformly disperse the treatment in the seeds. Then, Flo-Riteº 1706 (2.0 oz / cwt of seeds) and the seeds were agitated to disperse the polymer evenly in the seeds. Treated seeds were planted within 2-3 hours of treatment. The doses for these strains were specific for the strain and the experiment. Target application rates for MIC-99023 include 1.250% 2, 1.091, 1.25% 0, 1.001, 192, 1.2502, 1.003 and 2.553 CFU / seed. Target application rates for MIC-84414 included fam 5.01, 1.041, 5.091, 1.053, 5.093 and 1.095 CFU / seed. Target application rates for MIC-19994 included fam 1.01, 1.003 and 1.095 CFU / seed. The target application rate for the MIC-15870 was based on the weight of the biomass per seed weight and was 12 g / kg of seed.
[00933] [00933] A sandy clay and a commercial potting soil (Farfardº, Agawam, MA) were used in this experiment. The sandy clay was mixed in the proportion of 60% clay and 40% mortar sand (Northeast Nursery, Peabody, MA). Before mixing, both planting media were sieved through a 3/8 "square steel mesh to remove larger particles and debris. Variety 6 winter wheat seeds were treated with commercial fungicide and insecticide treatment. The seeds were heterologously arranged with the endophyte formulations and formulation control (without any endophyte), as described in Example 6, untreated seeds (lack of formulation and endophyte) were also planted in. Each pot was filled with 600 mL of its respective soil, watered with 200 mL of water and then nine seeds were sown evenly in each pot (in a 3x3 pattern) .The soil was then covered over the seeds (estimated average planting depth of 1 inch) and an additional 110 mL of water was added to moisten the overlapping soil substrate. The experimental design required a completely random pattern of each treatment within each block / replica. were defined in the 12h photoperiod, at a temperature of 22 / 18C during the day / night period and the light intensity was set at 650 µMol m sº. After planting, the seeds were watered to maintain approximately 80% of the soil's capacity.
[00934] [00934] The emergence of wheat seedlings was recorded on days 4, 5 and 7 after planting, with days 4 and 5 representing the percentage of early emergence and day 7 representing the percentage of final emergence. On day 7, all pots were diluted to 3 seedlings / pot. The above-ground tissue was harvested from the experiment three weeks after planting. The tissues of the individual treatments (vases) were collected and placed in an unlined paper bag. All fabrics were dried in an oven at 85 ° C for 3 days. After being completely dry, the shoot biomass of the individual treatment repetitions (pots) was weighed and recorded. Table 10. Dry biomass of sprout of wheat seedlings treated with endophytes grown on commercial land under normal irrigation conditions Biomass Control; Error = not EE EE average (g) Pp average A% overall | Untreated control | 0274] 0/0405) 000372] - 000) [eople - | 9263] o0387 | o005 | - ao Table 11. Dry shoot biomass of wheat seedlings treated with endophytes grown in sandy clay soil under normal irrigation conditions Biomass: Error = Control and ENE average (g) Mean Pp A% overall [Untreated control | 0378] 00405] 0007] oo) Example 8. Cultivation of plants treated with endophytes in the greenhouse experiment 4
[00935] [00935] A sandy clay and a commercial potting soil (Farfardº, Agawam, MA) were used in this experiment. The sandy clay was mixed in the proportion of 60% clay and 40% mortar sand (Northeast Nursery, Peabody, MA). Before mixing, both planting media were sieved through a 3/8 "square steel mesh to remove larger particles and debris. Variety 6 winter wheat seeds were treated with commercial fungicide and insecticide treatment. The seeds were heterologously arranged with endophyte formulations and formulation control (without any endophyte), as described in Example 6, except that LVMC was not used, untreated seeds (lack of formulation and endophyte) were also planted. applied to the seeds in three target doses: high (1095 CFU / seed), medium (1074 CFU / seed), low (10º3 CFU / seed). Each pot was filled with 600 mL of its respective soil, watered with 200 mL of water and then nine seeds were sown evenly in each pot (in a 3x3 pattern). The soil was then covered over the seeds (estimated average planting depth of 1 inch) and an additional 110 mL of water was added o to moisten the overlapping soil substrate. The experimental design required a completely random pattern of each treatment within each block / replica. The environmental conditions were defined in the 12h photoperdo, at the temperature of 22/18 during the day / night period and the light intensity was defined at 650 µMol m s!. After planting, the seeds were watered to maintain approximately 80% of the soil's capacity.
[00936] [00936] The emergence of wheat seedlings was recorded on days 4, 5 and 7 after planting, with days 4 and 5 representing the percentage of early emergence and day 7 representing the percentage of final emergence. On day 7, all pots were diluted to 3 seedlings / pot. The above-ground tissue was harvested from the experiment three weeks after planting. The tissues of the individual treatments (vases) were collected and placed in an unlined paper bag. All fabrics were dried in an oven at 85 ° C for 3 days. After being completely dry, the shoot biomass of the individual treatment repetitions (pots) was weighed and recorded.
[00937] [00937] Treatment with MIC-19994 resulted in a 6% increase (p <0.05) in dry biomass at 1074 CFU / seed.
[00938] [00938] Standard planting trays were used (Griffin, 1020 flat standard light weight, 4453-3325; inside a standard tray without holes (1020 flat open - without holes, f59-3175)). The commercial potting soil (Fafardº, Northeast Nursery, MA, t302101) was placed in the coating trays; the trays were soaked in water and the water was drained before planting. The wheat seeds were arranged heterologously with the endophyte formulations and the formulation control (without any endophyte), as described in Example 6, untreated seeds (lack of formulation and endophyte) were also planted. Each treatment contained 8 replicates of 16 seeds. The seedlings were scored for the emergency for 6 days. On day 8, seedlings were harvested; dried in the oven for 3 days at 80C and weighed.
[00939] [00939] All treatments with endophytes increased the dry weight of Prosper seedlings compared to controls. MIC-84414, MIC-19994 and MIC-99023 increased the dry weight of the seedlings in both Prosper and Briggs 5. MIC-15870 and MIC-19994 improved germination in both varieties with greater seed increases in Variety 2.
[00940] [00940] To prepare the culture as an initial inoculum for various assays, MIC-82330 was grown in one liter of 50% trypticase soy broth (TS) in a 2.5 liter Ultra Yield flask (Thomson Instrument Company). The culture was grown at 25 ° C under continuous agitation at a speed of 130 revolutions per minute (rpm) for five days. The cultures were aliquoted in 50 ml falcon tubes and were harvested by centrifugation at a speed of 3500 rpm for 20 minutes. The spent broth was decanted from the tubes, the cells were resuspended in 40 mM sodium phosphate buffer at pH 7. The bacterial titer was quantified using standard dilution plates and subsequently diluted as appropriate in 40 mM phosphate buffer to allow the desired target application rates per seed.
[00941] [00941] The spore solutions are made by washing and scraping the spores of the inclined agar, in which the MIC-19994 grows about 1 month ago. Washing is done with 0.05% Silwet. Solutions are passed through Miracloth to filter mycelia. Spores per ml are counted under a microscope using a hemocytometer. The stock suspension is then diluted to 10º6 spores / ml using water. 3 ul of spore suspensions are used per seed (-10º3 CFUs / seed are obtained) Control treatments are prepared by adding equivalent volumes of sterile water to the seeds.
[00942] [00942] The endophyte compositions were generated comprising an oil rich in erucic acid, a nonionic surfactant and a plantability polymer.
[00943] [00943] The treatments with endophytes were arranged heterologously for rice seeds according to one of the two different formulation protocols (Formulation Protocol A, Formulation Protocol B). A corresponding seed formulation control was also prepared, without any endophyte, for each type of formulation. Additional seeds without any formulation and endophyte were planted as an untreated baseline control. Formulation A included only diluent (0.05% silwet for MIC-19994, 40 mM phosphate buffer for MIC-82330) and microbial preparations. Formulation B included the same diluents listed above and the Flo Riteº 1706 seed plantability number applied to 2.0 oz / cwt of seeds by the manufacturer. Microbe and polymer were applied sequentially.
[00944] [00944] For endophytes formulated by Formulation Protocol A, microbial preparations were applied to the seeds at a rate of 1 μl / seed and the seeds were agitated for at least 20 seconds to disperse the microbe.
[00945] [00945] For endophytes formulated by Formulation Protocol B, microbial preparations were applied to the seeds at a rate of 1 uplL / seed. Then, the Flo Riteº 1706 plantability number was applied to the seeds at the manufacturer's suggestion (2.0 oz / cwt of seed) and stirred for 20 seconds to disperse the polymer.
[00946] [00946] Treated seeds were planted within 2-3 hours of treatment.
[00947] [00947] A sandy clay growth substrate was mixed in the greenhouse and consisted of 60% clay and 40% sand mortar (Northeast Nursery, Peabody, MA). Before mixing, the clay was sieved through a 3/8 "square mesh sieve to remove larger particles and debris. The rice seeds were treated with a fungicide treatment and commercial insecticide Apron XLG (Syngenta, Basel, Switzerland) from According to the manufacturer's instructions, the seeds were arranged heterologously with the endophyte formulations and the formulation control (without any endophyte), as described in Example 11, untreated seeds (lack of formulation and endophyte) were also planted. was filled with 600 mL of its respective soil, watered with 200 mL of water and then nine seeds were sown evenly in each pot (in a 3x3 pattern). The soil was then covered over the seeds (estimated average planting depth 1 inch) and an additional 110 mL of water was added to moisten the overlapping soil substrate.The experimental design required a completely random pattern of each treatment within each block / replica. The environmental conditions were defined in the 12h photoperiod, at a temperature of 22 / 18C during the day / night period and the light intensity was defined at 650 µMol m s!. After planting, the seeds were watered to maintain approximately 80% of the soil's capacity. The above-ground tissue was harvested from the experiment three weeks after planting. The tissues of the individual treatments (vases) were collected and placed in an unlined paper bag. All fabrics were dried in an oven at 85ºC for 3 days. After being completely dry, the shoot biomass of the individual treatment repetitions (pots) was weighed and recorded. Table 17. Dry root biomass of rice plants treated with endophytes and formulations In general,% watering hay 0 E CEEE root Dry root formulation in average (g) dwarf treated | Naotated | | oo | o [| At | | oo | 73% | [Bt oo | 82 | = | | oo6r | | 478 |
[00948] [00948] An agar buffer from each bacterial strain was transferred using a transfer tube to 4 mL of potato dextrose broth (PDB) in a 24-well plate and incubated at room temperature at 675 rpm on a shaker for 3 days. After the growth of bacteria in broth, 200 µl were transferred to a reading plate by a spectrophotometer and the OD was read with absorbance at 600 nm. All bacterial strains were then normalized to 0.05 OD using 1x PBS buffer. Once the desired dilutions were made, 3 µl of the bacterial solution per seed was applied and mixed well with shaking in a sterile falcon tube for 5-10 seconds. Preparation of fungal endophytes
[00949] [00949] Preparation of molasses broth and potato dextrose agar: The molasses broth was prepared by dissolving 30 g of molasses and 5 g of yeast extract per liter of deionized water in an autoclavable container and autoclaving it (15 psi, 121º ) for 45 min. Potato dextrose agar (PDA) plates were prepared by dissolving 39.0 g of powdered PDA per liter of deionized water in an autoclavable container and autoclaving it (15 psi, 121 ºC) for 45 min. The agar was allowed to cool to 50-60 ° C (before pouring it into sterile petri dishes (30 ml per 90 mm dish).
[00950] [00950] Biomass | liquid: All equipment and consumables have been completely sterilized and the procedures performed in a biosafety cabinet. The inoculant is prepared by placing 1 buffer of cryopreserved material in a fresh PDA plate, sealing the plate with Parafilm € 6 and incubating it at room temperature in the dark for 5 to 10 days. Then, the -5x5 mm plugs were cut from the PDA plates and the 10-12 plugs were transferred to vials containing the sterile molasses broth, then covered, fixed on a shaker and incubated for at least 10 days with shaking at -130 rpm. Then, the culture was placed in a blender for 5 seconds and 1 mL of the mixture was centrifuged and the supernatant was discarded and the pellet resuspended in 0.5 mL of phosphate buffered saline (PBS) 1x to generate inoculum.
[00951] [00951] Dry biomass: All equipment and consumables have been completely sterilized and the procedures performed in a biosafety cabinet. The inoculant is prepared by placing 1 buffer of cryopreserved material on a fresh PDA plate, sealing the plate with Parafilm € and incubating it at room temperature in the dark for 5 to 10 days. Then, the -5x5 mm plugs were cut from the PDA plates and the 10-12 plugs were transferred to vials containing the sterile molasses broth, then covered, fixed on a shaker and incubated for at least 10 days with shaking at -130 rpm. Under sterile conditions, culture | liquid was carefully decanted using sterile 150 mm filter paper in a sterile Buchner funnel over a sterile flask. After all | After passing through the funnel, the sediment was washed with sterile water until the filtrate was clean. When dry, the sediment was transferred to a drying cabinet and dried until it became brittle. The pellet was then ground into fine powder and the sample used to generate CFU counts.
[00952] [00952] A 2% weight / volume sodium alginate solution for the seed coatings is prepared by the following method. An Erlenmeyer flask is filled with the appropriate volume of deionized water and heated to 50 degrees Celsius on a hot plate with stirring using a stir bar. The appropriate mass of sodium alginate powder for the desired final concentration solution is slowly added until it dissolves. The solution is autoclaved at 121 degrees Celsius at 15 PSI for 30 minutes to sterilize.
[00953] [00953] Talcum powder for powdered seed coatings is prepared by the following method. The talcum powder is aliquoted in Ziploc bags or 50 mL falcon tubes, and autoclaved in a dry cycle (121 degrees Celsius at 15 PSI for 30 minutes) to sterilize.
[00954] [00954] Wheat seeds were treated with commercial fungicide and insecticide treatments. The seeds were arranged heterologously to each endophyte according to the following seed treatment protocols for formulation | wet or dry.
[00955] [00955] Formulation | liquid: The 2% sodium alginate solution prepared above was added to the seeds at a rate of 15 ml per kg of seeds. Fungal culture | Liquid as prepared above was added to the seeds at a rate of 8.3 ml per kg of seeds. Control treatments were prepared using equivalent volumes of sterile broth. The seeds were then stirred to disperse the solution evenly over the seeds.
[00956] [00956] Then, 12.5 g of talcum powder per kg of seed was added and the seeds were agitated to uniformly disperse the powder in the seeds. Then, 17 ml per kg of Flo-Riteº 1706 seed (BASF, Ludwigshafen, Germany) were added and the seeds were agitated to disperse the powder evenly in the seeds. The final concentration of endophyte was estimated to be at least 10% 4 CFU. The treated seeds were allowed to dry overnight in a well-ventilated space before planting.
[00957] [00957] Dry formulation: The 2% sodium alginate solution prepared above was added to the seeds at a rate of 20 ml per kg of seeds. Equal parts of the dried fungus biomass prepared above and the talc prepared above were mixed. The solution is applied to the prepared seeds so that an equivalent of 12.5 g of dry fungi biomass is applied per kg of seeds. Control treatments were prepared using equivalent volumes of talc. The seeds were then stirred to disperse the solution evenly over the seeds.
[00958] [00958] Then, 17 ml per kg of seed of Flo-
[00959] [00959] The soybean seeds of three soybean varieties were treated with a fungicide and commercial insecticide treatment, CruiserMaxxO (Syngenta, Basel, Switzerland), according to the manufacturer's instructions. The seeds were arranged heterologously to each endophyte according to the following seed treatment protocols for formulation | guided or dry.
[00960] [00960] Formulation | liquid: The 2% sodium alginate solution prepared above was added to the seeds at a rate of 8.3 ml per kg of seeds. Fungal culture | Liquid as prepared above was added to the seeds at a rate of 8.3 (fungal endophytes) or 8.4 (bacterial endophytes) ml per kg of seeds. Control treatments were prepared using equivalent volumes of sterile broth. The seeds were then stirred to disperse the solution evenly over the seeds. For fungal endophytes, g per kg of seed of the talc powder prepared above was added and the seeds were agitated to disperse the powder evenly in the seeds. Then, 13.3 (for treatments with fungal endophytes) or 2.7 (for treatments with bacterial endophytes) ml per kg of Flo-Riteº 1706 seed (BASF, Ludwigshafen, Germany) were added and the seeds were agitated to disperse the powder evenly on the seeds. The final concentration of endophyte was estimated to be at least 10% CFU. The treated seeds were allowed to dry overnight in a well-ventilated space before planting.
[00961] [00961] Dry fungal formulation: 2% sodium alginate was added to the seeds at a rate of 16.6 ml per kg of seeds. Equal parts of the dried fungus biomass prepared above and the talc prepared above were mixed. The solution was applied so that an equivalent of 10 g of dry fungal biomass was applied per kg of seeds. Control treatments were prepared using equivalent volumes of talc. The seeds were then stirred to disperse the solution evenly over the seeds.
[00962] [00962] Then, 13.3 ml were added! per kg of Flo-
[00963] [00963] Corn seeds were treated with commercial fungicide and insecticide treatment. The seeds were arranged heterologously to each endophyte according to the following seed treatment protocols for formulation | wet or dry.
[00964] [00964] Dry fungal formulation: The 2% sodium alginate solution prepared above was added to the seeds at a rate of 23 ml per kg of seeds. Equal parts of the dried fungus biomass prepared above and the talc prepared above were mixed. The solution was applied so that an equivalent of 10 g of fungal powder was applied per kg of seeds. Control treatments were prepared using equivalent volumes of talc. The seeds were then stirred to disperse the solution evenly over the seeds.
[00965] [00965] Then, 16.6 ml were added! per kg of Flo-Riteº 1706 seed (BASF, Ludwigshafen, Germany) and the seeds were agitated to disperse the powder evenly in the seeds. The final concentration of endophyte was estimated to be at least 104 CFU. The treated seeds were allowed to dry overnight in a well-ventilated space before planting.
[00966] [00966] Formulation | flow: Culture | liquid as prepared above was added to the seeds at a rate of 23 (for treatments with fungal endophytes) or 8.4 (for treatments with bacterial endophytes) ml! per kg of seeds, with equivalent volumes of the prepared sodium alginate. Control treatments were prepared using equivalent volumes of sterile broth. The seeds were then stirred to disperse the solution evenly over the seeds. For fungal endophytes, 15 g per kg of seed of talc powder prepared in sterile were added and the seeds were agitated to disperse the powder evenly in the seeds. Then, 16.6 ml (for treatments with fungal endophytes) or 2.4 ml (for treatments with bacterial endophytes) per kg of Flo-Riteº 1706 seed (BASF, Ludwigshafen, Germany) were added and the seeds were agitated to disperse the powder evenly in the seeds. The final concentration of endophyte was estimated to be at least 104 CFU. The treated seeds were allowed to dry overnight in a well-ventilated space before planting.
[00967] [00967] Field tests were conducted at 6 different locations in the Midwest region of the United States in 2016, under non-irrigated conditions (dry land). Four varieties of spring wheat seeds were treated with commercial fungicide and insecticide treatment. The seeds were arranged heterologously with the endophyte formulations and the formulation control (without any endophyte), as described in Example 14, untreated seeds (lack of formulation and endophyte) were also planted. Two varieties were planted at each location. The seeds were sown in rows regularly spaced in the soil, with a density of 1.2 million seeds / acres. At each site, 4 replicated lots were planted by endophyte treatment or control in a randomized complete block design. Each lot consisted of seven 15.24 m (40 ft) rows.
[00968] [00968] At the end of the field trial, using treatment with endophytes and control treatment plants, the batches were harvested by machine with a combination of 5-foot survey and the yield was calculated by the on-board computer.
[00969] [00969] Field trials were conducted in 2016, under non-irrigated conditions (dry land). Two varieties of spring wheat seeds were treated with commercial fungicide and insecticide treatment. The seeds were arranged heterologously with the endophyte formulations and the formulation control (without any endophyte), as described in Example 14, untreated seeds (lack of formulation and endophyte) were also planted. The seeds were sown in rows regularly spaced in the soil, with a density of 1.2 million seeds / acres. At each site, replicated lots were planted for each treatment with endophyte or control in a randomized complete block design. Each lot consisted of seven 15.24 m (40 ft) rows.
[00970] [00970] At the end of the field trial, employing treatment with endophytes and control treatment plants, the batches were harvested by machine with a combination of 5-foot survey and the grain yield and percent moisture were calculated by the on-board computer . Table 27. Yield by spring wheat treated with endophyte and untreated control and formulation control, field experiment 2 - EEZ = EE Variety | Yield variety | treated | of 1: 2,; (bu / acre)% formula yield | yield tion A% (bu / acre) (bu / acre) general general according to the treated Control of 31.172 28.217 29.69 15.4 starvation to Example 17. Cultivation of plants treated with endophytes in field experiment 3
[00971] [00971] Field trials were conducted in 2016, under non-irrigated conditions (dry land). Four varieties of wheat seeds were treated with commercial fungicide and insecticide treatment. The seeds were arranged heterologously with the endophyte formulations and the formulation control (without any endophyte), as described in Example 14, untreated seeds (lack of formulation and endophyte) were also planted. The seeds were sown in rows regularly spaced in the soil, with a density of 1.2 million seeds / acres. At each site, replicated lots were planted for each treatment with endophyte or control in a randomized complete block design. Each lot consisted of seven 15.24 m (40 ft) rows.
[00972] [00972] At the end of the field trial, using treatment with endophytes and control treatment plants, the batches were harvested by machine with a combination of 5-foot survey and the yield was calculated by the on-board computer. Table 28. Yield by variety of wheat treated with endophyte and formulation control and untreated control, field experiment 3
[00973] [00973] Field trials were conducted in 2016, under non-irrigated conditions (dry land). The wheat seeds were arranged heterologously with the endophyte formulations and the formulation control (without any endophyte), as described in Example 14, untreated seeds (lack of formulation and endophyte) were also planted. The seeds were sown in rows regularly spaced in the soil, with a density of 1.2 million seeds / acres. At each site, replicated lots were planted for each treatment with endophyte or control in a randomized complete block design. Each lot consisted of seven 15.24 m (40 ft) rows.
[00974] [00974] At the end of the field trial, using treatment with endophytes and control treatment plants, the batches were harvested by machine with a 5-foot survey combination and the yield was calculated by the on-board computer. Table 29. Yield per wheat site treated with endophyte and untreated control and formulation control, field experiment 4 2 2 | & | 2 2 2 2 sl8 É É É É É É E S / l = Ex | ÉEZ | ES Ex | Ex | Ex | Ex (E) 23) Éçs | 8 <28 SE 58 5S | 3 358 / = 3E [SE ST 35/2885 SS | 5SS / SS) 5S | SS | 5SS | healthy | 5sS | / 528/25 | / 98S e ". - 3 3 3 - 3 e. 3 -3 | gs) .X | 2305 SE | S2 / Y2 / 52 | 028 | r 2 | = S2 | S2L | HE / Fa | SE 3 3 E | 3 3 3 3 BlE | ºs oooooooooo = = - - = -. = = RjO [cont] 354 | 342] 252 | 877 | 399 | 41 | 405 [292/4176] | 12 )
[00975] [00975] Field trials were conducted in 2016, under non-irrigated conditions (dry land). Wheat seeds were treated with commercial fungicide and insecticide treatment. The seeds were arranged heterologously with the endophyte formulations and the formulation control (without any endophyte), as described in Example 14, untreated seeds (lack of formulation and endophyte) were also planted. The seeds were sown in rows regularly spaced in the soil, with a density of 1.2 million seeds / acres. At each site, at least 3 replicate lots were planted for each treatment with endophyte or control in a randomized complete block design. Each lot consisted of seven 15.24 m (40 ft) rows.
[00976] [00976] At the end of the field trial, using treatment with endophytes and control treatment plants, the batches were harvested by machine with a 5-foot survey combination and the yield was calculated by the on-board computer. Corn
[00977] [00977] Field trials were conducted at 5 different locations in the south, west and midwest regions of the United States in 2016. Plots of land were either not irrigated (dry land) or were maintained with less than ideal irrigation at a rate which aimed at a reduction of approximately 25% in income. The seeds were prepared with the endophyte formulations and formulation control (without any endophyte) as described in Example 14. The seeds were sown in rows spaced evenly in the soil at typical planting densities for each region. At each site, 3 replicated lots were planted by endophyte treatment or control in a randomized complete block design. Each lot consisted of four rows of 15.24 m (40 feet), each separated by 76.2 cm (30 inches).
[00978] [00978] At the end of the field trial, employing treatment with endophytes and control treatment plants, the batches were harvested using a 5-foot survey combination and the yield was calculated by the on-board computer. Only the two rows in the middle of the four rows were harvested to show border effects.
[00979] [00979] Field tests were conducted in 4 different locations in the Midwest region of the United States in 2016, under non-irrigated conditions (dry land). The seeds were prepared with the endophyte formulations and formulation control (without any endophytes) as described in Example 14. The seeds were sown in regularly spaced rows in the soil, with a density of 180,000 seeds / acres. At each site, 4 replicated lots were planted by endophyte treatment or control in a randomized complete block design. Each lot consisted of four rows of 15.24 m (40 feet), each separated by 76.2 cm (30 inches).
[00980] [00980] At the end of the field trial, employing treatment with endophytes and control treatment plants, the batches were harvested using a 5-foot survey combination and the yield was calculated by the on-board computer. Only the two rows in the middle of the four rows were harvested to prevent edge effects.
[00981] [00981] Samples of seeds from harvested plants are obtained as described in Example 19. Fat analysis is performed on replicated samples according to the Official Agricultural Chemists Reference Method AOAC method
[00982] [00982] Samples of seeds from harvested plants are obtained as described in Example 19. Fat analysis is performed on replicated samples according to the Official Agricultural Chemists Reference Method ADAC method
[00983] [00983] Samples of seeds from harvested plants are obtained as described in Example 19. Fat analysis is performed on replicated samples according to the Official Agricultural Chemists Reference Method AOAC method
[00984] [00984] Samples of seeds from harvested plants are obtained as described in Example 19. Fat analysis is performed on replicated samples according to the Official Agricultural Chemists Reference Method AOAC method
[00985] [00985] Samples of harvested plant seeds are obtained as described in Example 19. Fat analysis is performed on replicated samples according to the Official Agricultural Chemists Reference Method AOAC method
[00986] [00986] Samples of seeds from harvested plants are obtained as described in Example 19. The carbohydrate analysis is determined for replicated samples as a calculation according to the following formula: Total Carbohydrate = 100% -% (Prote ha + Ash + Fat + Humidity +
[00987] [00987] Seed samples from harvested plants are obtained as described in Example 19. Calorie analysis is determined for replicated samples as a calculation according to the following formula: Total calories = (protein calories) + (carbohydrate calories) ) + calories from fat) When the calories from protein are calculated as 4 calories per gram of protein (as determined by the method of Example 24), the calories from carbohydrate are calculated as 4 calories per gram of carbohydrate (as determined by the method from Example 25), and the calories from fat are calculated as 9 calories per gram of fat (as determined according to the method of Example 20). The average percentage changes between treatment (seed treated with endophyte) and control (seed treated with the formulation, but without endophyte) are calculated.
[00988] [00988] A fungal or bacterial endophyte is inoculated into seeds during the process of osmoconditioning (immersion in polyethylene glycol solution! To create a range of osmotic potentials) and / or hydroconditioning - (immersion in chlorinated water). a polyethylene glycol solution containing a bacterial and / or fungal endophyte for one to eight days and then air dried for one to two days. Hydroconditioned seeds are soaked in water for one to eight days containing a bacterial and / or fungal endophyte and kept under constant aeration to maintain an adequate content of dissolved oxygen from the suspension until removal and air drying for one to two days. Talc and / or fluidity polymer are added during the drying process.
[00989] [00989] A fungal or bacterial endophyte is inoculated into the above-ground plant tissue (leaves and stems) as a suspension | in decolorized water containing adjuvants, adhesive spreaders and UV protectors. The suspension is sprayed on cultures with a lance or other suitable spray.
[00990] [00990] A fungal or bacterial endophyte is inoculated into soil in the form of a suspension | liquid; pre-planting as a puddle of soil, during planting as a furrow application, or during crop growth as a side cover. A fungal or bacterial endophyte is mixed directly into a fertigation system via drip, center pivot or other appropriate irrigation system.
[00991] [00991] A fungal or bacterial endophyte is inoculated into a hydroponic or aeroponic system, such as a powder suspension or | liquid, applied directly to the rock substrate, or applied to the circulating or pulverized nutrient solution.
[00992] [00992] A fungal or bacterial endophyte is introduced in powder form into a mixture containing talc or other bulking agent at the entrance to a hive (in the case of mediation of bees) or near the nest of another pollinator (in the case of other insects or birds). Pollinators pick up the dust when they leave the hive and deposit the inoculum directly on the crop's flowers during the pollination process.
[00993] [00993] The method includes contacting the outer surface of the branches of a plant with an inoculant formulation | liquid containing a purified bacterial population, a purified fungal population or a mixture of purified bacteria and fungi. The plant's roots are passed briefly through the microbial formulation | permanent liquid or formulation | The liquid is sprayed freely on the roots, resulting in the physical removal of soil and microbial debris from the roots of the plant, as well as inoculation with microbes in the formulation.
[00994] [00994] The method includes contacting the outer surfaces of a seedling with an inoculant formulation | liquid containing a purified bacterial population, a purified fungal population or a mixture of purified bacteria and fungi. The entire seedling is immersed in microbial formulation | permanent liquid for at least 30 seconds, resulting in the physical removal of soil and microbial debris from plant ragkes, as well as in inoculation of all plant surfaces with microbes in the formulation. Alternatively, the seedling can be germinated from the seed or transplanted in media soaked with the microbe (s) of interest and then left to grow in the media, resulting in immersion of the seedling in the microbial formulation for a much longer time, totaling even days or weeks. Endophytic microbes probably need time to colonize and enter the plant, while exploring the plant's surface for cracks or wounds to enter, so the longer the soak time, the more likely the microbes will be successfully installed on the plant.
[00995] [00995] The method includes contacting the injured surface of a plant with an inoculant formulation | liquid or solid containing a purified bacterial population, a purified fungal population, or a mixture of purified bacteria and fungi. Plant surfaces are designed to block microbes from entering the endosphere, as pathogens try to infect plants in this way. To introduce beneficial endophytic microbes into plant endospheres, we need a way to access the interior of the plant, which we can do by opening a passage through wounds. This wound can take a variety of forms, including pruned branches, pruned branches, perforations in the stem that break the bark and cortex, perforated wounds in the primary root, perforated wounds in the leaves and perforated wounds allowing entry after the seed coating. Wounds can be made with needles, hammer and nails, knives, drills etc. In the wound, the microbial inoculant can then be contacted as | liquid, like powder, inside gelatin capsules, in a pressurized capsule injection system, in a reservoir and piping pressurized injection system, allowing entry and colonization by microbes in the endosphere. Alternatively, the entire injured plant can be soaked or washed in the microbial inoculant for at least 30 seconds, giving more microbes the chance to enter the wound, as well as inoculating other plants with microbes in the formulation - for example, pruning seedlings and soaking them in inoculants before transplantation is a very effective way to introduce endophytes into the plant.
[00996] [00996] The method includes the injection of microbes in a plant to successfully install them in the endosphere. Plant surfaces are designed to block microbes from entering the endosphere, as pathogens try to infect plants in this way. To introduce beneficial endophytic microbes into the endospheres, we need a way to access the interior of the plant, which we can do by piercing the surface of the plant with a needle and injecting microbes into the plant. Different parts of the plant can be inoculated in this way, including the main trunk or trunk, branches, primary branches, seminal branches, buttress parts and even leaves. The injection can be done with a hypodermic needle, a drilled orifice or a specialized injection system, and through the wound the microbial inoculant, such as | fluid, in powder, inside gelatin capsules, in a pressurized capsule injection system in a pressurized reservoir and pipeline injection system, allowing entry and colonization by microbes in the endosphere.
[00997] [00997] Phylogenomic analysis of entire genomic sequences of endophytes can be used to identify distinctive sequence variants. Gene sets suitable for phylogenomic analysis, as well as methods for identifying them, are well known in the art, for example Floutas et al. (2012) The Paleozoic origin of enzymatic lignin decomposition reconstructed from 31 fungal genomes. Science, 336 (6089): 1715-9. doi: 10.1126 / science 1221748 and James TY, Pelin A, Bonen L, Ahrendt S, Sain D, Corradi N, Stajich JE.Shared signatures of parasitism and phylogenomics unite Cryptomycota and microsporidia. Curr Biol.
[00998] [00998] Analysis of the complete genome of endophytes can be used to identify genes whose presence, absence or supra or underrepresentation ("differential abundance") is associated with desirable phenotypes. To identify genes with differential abundance in the genome of an endophyte of interest, predicted protein sequences from the endophyte genomes and closely related species are compared in a peer-to-peer comparison of all with all (for example, using BLAST), followed by clustering of protein sequences based on alignment scores (for example, using MCL: Enright AJ, Van Dongen S., Ouzounis CA An efficient algorithm for large-scale detection of protein families. Nucleic Acids Research 30 (7) : 1575-1584 (2002)). Additional software tools useful for this analysis are well known in the art and include OMA, OrthoMCL and TribeMCL (Roth AC, Gonnet GH, Dessimoz C. Algorithim of OMA for large-scale orthology inference. BMC Bioinformatics. 2008; 9: 518. doi: 10.1186 / 1471-2105-9-518, Enright AJ, Kunin V, Ouzounis CA. Protein families and TRIBES in genome sequence space.
[00999] [00999] Having illustrated and described the principles of the present invention, it should be evident to persons skilled in the art that the invention can be modified in the arrangement and details, without deviating from such principles. It should be understood that although the invention has been described in conjunction with its detailed description, the foregoing description is intended to illustrate and not to limit the scope of the invention, which is defined by the scope of the appended claims. Other modalities, advantages and modifications are within the scope of the following claims.

权利要求:
Claims (8)
[1]
1. Method of improving a trait of agronomic importance in a soybean plant, characterized by the fact that it comprises having, in a heterologous way, an endophyte organism for an element of the soybean plant in an effective amount to increase a trait of importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 32 and 33.
[2]
2. Method of improving a trait of agronomic importance in a soybean plant, characterized by the fact that it comprises, in a heterologous way, an endophyte organism for an element of the soybean plant in an effective amount to increase a trait of agronomic importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia, as deposited under o NRRL Culture Deposit No. 67466.
[3]
3. Method of improving a trait of agronomic importance in a soybean plant, characterized by the fact that it comprises having, in a heterologous way, an endophyte organism for an element of the soybean plant in an effective amount to increase a trait of importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a modified endophyte organism of the genus Periconia derived endophyte organism, as deposited under NRRL Culture Deposit No. 67466.
[4]
4. Method for improving a trait of agronomic importance in a wheat plant, characterized by the fact that it comprises having, in a heterologous way, an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of importance agronomic selected from the group consisting of germination rate, dry shoot biomass and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 32 and
33.
[5]
5. Method for improving a trait of agronomic importance in a wheat plant, characterized by the fact that it comprises, in a heterologous way, an endophyte organism for an element of the wheat plant in an effective amount to increase a trait of agronomic importance selected from the group consisting of germination rate, dry shoot biomass and yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466.
[6]
6. Method of improving a trait of agronomic importance in a wheat plant, characterized by the fact that it comprises having, in a heterologous way, an endophyte organism for an element of the wheat plant in an effective amount to increase a trait of importance selected from the group consisting of root length and root area in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a modified endophyte organism of the genus Periconia derived endophyte organism, as deposited under NRRL Culture Deposit No. 67466.
[7]
7. Method of enhancing a trait of agronomic importance in a corn plant, characterized by the fact that it comprises having, in a heterologous way, an endophyte organism for an element of the corn plant in an effective amount to increase the yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus
Periconia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 32 and 33.
[8]
8. Method of improving a trait of agronomic importance in a corn plant, characterized by the fact that it comprises, in a heterologous way, an endophyte organism for an element of the corn plant in an amount effective to increase the yield in the derived plant of the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466.
9. Method of improving a trait of agronomic importance in a corn plant, characterized by the fact that it comprises having, in a heterologous way, an endophyte organism for an element of the corn plant in an effective amount to increase the yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67466.
10. Method for improving a trait of agronomic importance in a soybean plant, characterized by the fact that it comprises having, in a heterologous way, an endophyte organism for an element of the soybean plant in an effective amount to increase a trait of importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38.
11. Method of enhancing a trait of agronomic importance in a soybean plant, characterized by the fact that it comprises, in a heterologous way, an endophyte organism for an element of the soybean plant in an effective amount to increase a trait of agronomic importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467.
12. Method of enhancing a trait of agronomic importance in a soybean plant, characterized by the fact that it comprises having, in a heterologous way, an endophyte organism for an element of the soybean plant in an effective amount to increase a trait of importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under NRRL Culture Deposit No.
67467.
13. Method of enhancing a trait of agronomic importance in a wheat plant, characterized by the fact that it comprises having, in a heterologous way, an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of importance selected from the group consisting of root length, root area and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38.
14. Method of improving a trait of agronomic importance in a wheat plant, characterized by the fact that it comprises, in a heterologous way, an endophyte organism for an element of the wheat plant in an effective amount to increase a trait of agronomic importance selected from the group consisting of root length and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia, as deposited under the Deposit NRRL Culture No.
67467.
15. Method for improving a trait of agronomic importance in a wheat plant, characterized by the fact that it comprises having, in a heterologous way, an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of importance selected from the group consisting of root length and yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the organism endophyte, as deposited under NRRL Culture Deposit No. 67467.
16. Method of enhancing a trait of agronomic importance in a corn plant, characterized by the fact that it comprises having, in a heterologous way, an endophyte organism for an element of the corn plant in an effective amount to increase the yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38.
17. Method for improving a trait of agronomic importance in a corn plant, characterized by the fact that it comprises, in a heterologous way, an endophyte organism for an element of the corn plant in an amount effective to increase the yield in the derived plant of the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467.
18. Method of enhancing a trait of agronomic importance in a corn plant, characterized by the fact that it comprises having, in a heterologous way, an endophyte organism for an element of the corn plant in an effective amount to increase the yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a modified endophyte organism of the genus Curvularia, derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67467.
19. Method for improving a trait of agronomic importance in a wheat plant, characterized by the fact that it comprises having, in a heterologous way, an endophyte organism for an element of the wheat plant in an effective amount to increase the yield in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleotide sequence at least 97% identical to SEQ ID NO: 39.
20. Method for improving a trait of agronomic importance in a wheat plant, characterized by the fact that it comprises having, in a heterologous way, an endophyte organism for an element of the wheat plant in an effective amount to increase a trait of importance selected from the group consisting of germination rate, root length, dry shoot biomass and yield on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 40-43.
21. Method of improving a trait of agronomic importance in a rice plant, characterized by the fact that it comprises having, in a heterologous way, an endophyte organism for an element of the rice plant in an effective amount to increase the root biomass dried plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleotide sequence at least 97% identical to a selected polynucleotide sequence among the group consisting of SEQ ID NOs: 40-43.
22. Method of enhancing a trait of agronomic importance in a wheat plant, characterized by the fact that it comprises having, in a heterologous way, an endophyte organism for an element of the wheat plant in an amount effective to increase a trait of importance selected from the group consisting of germination rate and dry shoot biomass in the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 44-48.
23. Method of enhancing a trait of agronomic importance in a rice plant, characterized by the fact that it comprises having, in a heterologous way, an endophyte organism for an element of the rice plant in an amount effective to increase the biomass of the sprout dried on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleotide sequence at least 97% identical to a selected polynucleotide sequence of the group consisting of SEQ ID NOs: 49-51.
24. Method of improving a trait of agronomic importance in a rice plant, characterized by the fact that it comprises, in a heterologous way, an endophyte organism for an element of the corn plant in an amount effective to increase dry root biomass on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a member of the genus Enterobacter, as deposited under NRRL Culture Deposit No. B67465.
25. Method for improving a trait of agronomic importance in a rice plant, characterized by the fact that it comprises, in a heterologous way, an endophyte organism for an element of the rice plant in an amount effective to increase the root biomass dry on the plant derived from the treated plant element relative to a plant derived from a reference plant element, where the endophyte organism is a modified endophyte organism of the genus Enterobacter, derived from the endophyte organism, as deposited under the NRRL Culture Deposit No. B67465.
26. Method according to any one of claims 1 to 25, characterized in that the plant element is a seed.
27. Method according to claim 26, characterized by the fact that the seed is modified.
28. Method according to any one of claims 1 to 25, characterized in that the endophyte organism is arranged heterologously to the plant element in a formulation that further comprises one or more of the following: a stabilizer, a preservative, carrier, surfactant, fungicide, nematicide, bactericide, insecticide or herbicide, or any combination thereof.
29. Method according to any one of claims 1, 4, 7, 10, 13, 19, 20, 21, 22 or 23, characterized by the fact that the percentage of identity is at least 98%.
30. Method according to any one of claims 1, 4, 7, 10, 13, 19, 20, 21, 22 or 23, characterized by the fact that the percentage of identity is at least 99%.
31. Method according to any one of claims 1, 4, 7, 10, 13, 19, 20, 21, 22 or 23, characterized by the fact that the identity percentage is 100%.
32. Method according to any one of claims 1, 4, 7, 10, 13, 19, 20, 21, 22 or 23, characterized in that the percentage of identity is determined over a region of alignment of at least 100 nucleotides.
33. Method according to any one of claims 1, 4, 7, 10, 13, 19, 20, 21, 22 or 23, characterized by the fact that the percentage of identity is determined over a region of alignment of at least 200 nucleotides.
34. Method according to any one of claims 1, 4, 7, 10, 13, 19, 20, 21, 22 or 23, characterized by the fact that the percentage of identity is determined over a region of alignment of at least
300 nucleotides.
35. Method according to any one of claims 1, 4, 7, 10, 13, 19, 20, 21, 22 or 23, characterized in that the percentage of identity is determined over a region of alignment of at least 400 nucleotides.
36. Method according to any one of claims 1, 4, 7, 10, 13, 19, 20, 21, 22 or 23, characterized in that the percentage of identity is determined over a region of alignment of at least 500 nucleotides.
37. Agrochemically active microbial formulation, characterized by the fact that it comprises at least one oil, surfactant, polymer and a microbial active ingredient, in which the microbial active ingredient is an endophyte organism of the genus Periconia and comprises at least one polynucleotide sequence at least 97 % identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 32 and
33.
38. Agrochemically active microbial formulation, characterized by the fact that it comprises at least one oil, surfactant, polymer and a microbial active ingredient, in which the microbial active ingredient is an endophytic organism of the genus Periconia, as deposited under the NRRL Culture Deposit No. 67466.
39. Agrochemically active microbial formulation, characterized by the fact that it comprises at least one oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under the Deposit NRRL Culture No. 67466.
40. Agrochemically active microbial formulation, characterized by the fact that it comprises at least one oil, surfactant, polymer and a microbial active ingredient, in which the microbial active ingredient is an endophytic organism of the genus Curvularia and comprises at least one polynucleotide sequence at least 97 % identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 34-38.
41. Agrochemically active microbial formulation, characterized by the fact that it comprises at least one oil, surfactant, polymer and a microbial active ingredient, in which the microbial active ingredient is an endophyte organism of the genus Curvularia, as deposited under the NRRL Culture Deposit No. 67467.
42. Agrochemically active microbial formulation, characterized by the fact that it comprises at least one oil, surfactant, polymer and an active microbial ingredient, in which the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under the Deposit NRRL Culture No. 67467.
43. Agrochemically active microbial formulation, characterized by the fact that it comprises at least one oil, surfactant, polymer and a microbial active ingredient, in which the microbial active ingredient is an endophyte organism of the genus Aspergillus and comprises at least one polynucleotide sequence at least 97 % identical to SEQ ID NO: 39.
44, Agrochemically active microbial formulation, characterized by the fact that it comprises at least one oil, surfactant, polymer and a microbial active ingredient, in which the microbial active ingredient is an endophyte organism of the genus Coniochaeta and comprises at least one polynucleotide sequence at least 97 % identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 40-43.
45. Agrochemically active microbial formulation, characterized by the fact that it comprises at least one oil, surfactant, polymer and a microbial active ingredient, in which the microbial active ingredient is an endophyte organism of the genus Enterobacter and comprises at least one polynucleotide sequence at least 97 % identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 49-51.
46. Agrochemically active microbial formulation, characterized by the fact that it comprises at least one oil, surfactant, polymer and a microbial active ingredient, in which the microbial active ingredient is an endophyte organism of the genus Enterobacter, as deposited under the NRRL Culture Deposit No. B67465.
47. Agrochemically active microbial formulation, characterized by the fact that it comprises at least one oil, surfactant, polymer and a microbial active ingredient, in which the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under the Deposit NRRL Culture No. B67465.
48. Formulation according to any of claims 37-47, characterized in that it is oil is rapeseed or NEEM.
49. Formulation according to any of claims 37-47, characterized in that the oil comprises erucic acid.
50. Formulation according to any of claims 37-47, characterized by the fact that the oil comprises herbicidal or insecticidal properties.
51. Formulation according to any of claims 37-47, characterized by the fact that the surfactant is a non-ionic detergent.
52. Formulation according to any of claims 37-47, characterized in that the surfactant is Tween 20 or Triton X-100.
53. Formulation according to any of claims 37-47, characterized by the fact that the polymer is FloRite &, DISCOTYou KannarO Universal Wonder.
54. Formulation according to any of claims 37-47, characterized by the fact that the microbial active ingredient comprises a spore suspension.
55. Formulation according to any of claims 37-47, characterized by the fact that the microbial active ingredient comprises spray dried spores.
56. Formulation according to any of claims 37-47, characterized in that the microbial active ingredient comprises whole cell broth.
57. Formulation according to any one of claims 37-47, characterized in that it further comprises one of the following: fungicide, nematicide, bactericide, insecticide or herbicide.
58. Formulation according to any of claims 37-47,
characterized by the fact that it also comprises one of the following: stabilizer, preservative, carrier, anti-complex agent or any combination of these.
59. Formulation according to any of claims 37-47, characterized by the fact that the endophyte organism is self-stable.
60. Formulation according to any of claims 37, 40, 43, 44 or 45, characterized by the fact that the percentage of identity is at least 98%.
61. Formulation according to any of claims 37, 40, 43, 44 or 45, characterized by the fact that the identity percentage is at least 99%.
62. Formulation according to any of claims 37, 40, 43, 44 or 45, characterized by the fact that the identity percentage is 100%.
63. Formulation according to any one of claims 37, 40, 43, 44 or 45, characterized by the fact that the percentage of identity is determined over an alignment region of at least 100 nucleotides.
64. Formulation according to any of claims 37, 40, 43, 44 or 45, characterized in that the percentage of identity is determined over an alignment region of at least 200 nucleotides.
65. Formulation according to any of claims 37, 40, 43, 44 or 45, characterized in that the percentage of identity is determined over an alignment region of at least 300 nucleotides.
66. Formulation according to any one of claims 37, 40, 43, 44 or 45, characterized by the fact that the percentage of identity is determined over an alignment region of at least 400 nucleotides.
67. Formulation according to any of claims 37, 40, 43, 44 or 45, characterized in that the percentage of identity is determined over an alignment region of at least 500 nucleotides.
68. Synthetic composition characterized by the fact that it comprises a plant element and an endophyte organism arranged in a heterologous manner, in which the endophyte organism is a member of the genus Periconia and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 32 and 33, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to the reference plant element that does not additionally comprise the endophyte organism.
69. Composition - synthetic according to claim 68, characterized by the fact that the endophyte organism comprises polynucleotide sequences at least 97% identical to each of the sequences in the group consisting of SEQ ID NOs: 32 and 33.
70. Synthetic composition characterized by the fact that it comprises a plant element and an endophyte organism arranged in a heterologous way, in which the endophyte organism is a member of the genus Periconia, as deposited under NRRL Culture Deposit No. 67466, in which the composition Synthetic is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism.
71. Synthetic composition characterized by the fact that a plant element and an endophyte organism arranged in a heterologous way, in which the endophyte organism is a modified endophyte organism of the genus Periconia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67466 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism.
72. Synthetic composition according to any one of claims 68-71, characterized by the fact that the endophyte organism of the taxonomy Periconia macrospinosa.
73. Synthetic composition according to any one of claims 68-71, characterized by the fact that the plant element is of the genus Triticum and the enhanced trait of agronomic importance is selected from the group consisting of the germination rate, biomass of dry sprout and yield.
74. Synthetic composition according to any one of claims 68-71, characterized by the fact that the plant element is of the genus Glycine and the enhanced trait of agronomic importance is selected from the group consisting of the root length and area of root.
75. Synthetic composition according to any one of claims 68-71, characterized by the fact that the plant element is of the genus Zea and the improved trait of agronomic importance is yield.
76. Synthetic composition, characterized by the fact that it comprises a plant element and an endophyte organism arranged in a heterologous way, in which the endophyte organism is a member of the genus Curvularia and comprises at least one polynucleotide sequence at least 97% identical to a sequence polynucleotide selected from the group consisting of SEQ ID NOs: 34-38, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism.
77. Synthetic composition according to claim 76, characterized by the fact that the endophyte organism comprises polynucleotide sequences at least 97% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 34-38.
78. Synthetic composition according to claim 76, characterized by the fact that the endophyte organism comprises polynucleotide sequences at least 97% identical to at least three sequences selected from the group consisting of SEQ ID NOs: 34-38.
79. Synthetic composition according to claim 76, characterized by the fact that the endophyte organism comprises polynucleotide sequences at least 97% identical to at least four sequences selected from the group consisting of SEQ ID NOs: 34-38.
80. Synthetic composition according to claim 76,
characterized by the fact that the endophyte organism comprises polynucleotide sequences at least 97% identical to at least five sequences selected from the group consisting of SEQ ID NOs: 34-38.
81. Synthetic composition characterized by the fact that it comprises a plant element and an endophyte organism arranged in a heterologous way, in which the endophyte organism is a member of the genus Curvularia, as deposited under NRRL Culture Deposit No. 67467, in which the composition Synthetic is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism.
82. Synthetic composition characterized by the fact that a plant element and an endophyte organism arranged in a heterologous manner, in which the endophyte organism is a modified endophyte organism of the genus Curvularia derived from the endophyte organism, as deposited under NRRL Culture Deposit No. 67467 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism.
83. Synthetic composition according to any one of claims 76-82, characterized by the fact that the referred endophyte organism is of the taxonomy Curvularia spicifera.
84. Synthetic composition according to any of claims 76-82, characterized by the fact that the plant element is of the genus Triticum and the enhanced trait of agronomic importance is selected from the group consisting of root length and yield.
85. Synthetic composition according to any of claims 76-82, characterized by the fact that the plant element is of the genus Glycine and the enhanced trait of agronomic importance is selected from the group consisting of root length, rate of germination and yield.
86. Synthetic composition according to any of claims 76-82, characterized by the fact that the plant element is of the genus Zea and the improved trait of agronomic importance is yield.
87. Synthetic composition characterized by the fact that it comprises a plant element and an endophyte organism arranged in a heterologous way, in which the endophyte organism is a member of the genus Aspergillus and comprises at least one polynucleotide sequence at least 97% identical to SEQ ID NO : 39, in which the aforementioned synthetic composition is able to provide an improved trait of agronomic importance compared to the element of the reference plant that does not additionally comprise the endophyte organism.
88. Synthetic composition, according to claim 87, characterized by the fact that the referred endophyte organism belongs to the Aspergillus ruber taxonomy.
89. Synthetic composition according to any of claims 87-88, characterized by the fact that the plant element is of the genus Triticum and the improved trait of agronomic importance is yield.
90. Synthetic composition characterized by the fact that it comprises a plant element and an endophyte organism arranged in a heterologous manner, in which the endophyte organism is a member of the genus Coniochaeta and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 40-43, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism.
91. Synthetic composition according to claim 90, characterized by the fact that the endophyte organism comprises polynucleotide sequences at least 97% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 40-43.
92. Synthetic composition according to claim 90, characterized by the fact that the endophyte organism comprises polynucleotide sequences at least 97% identical to at least three sequences selected from the group consisting of SEQ ID NOs: 40-43.
93. Synthetic composition according to claim 90, characterized by the fact that the endophyte organism comprises polynucleotide sequences at least 97% identical to at least four sequences selected from the group consisting of SEQ ID NOs: 40-43.
94. Synthetic composition according to any of claims 90-93, characterized by the fact that the endophyte organism belongs to the taxonomy Coniochaeta prunicola.
95. Synthetic composition according to any of claims 90-93, characterized by the fact that the plant element is of the genus Triticum and the enhanced trait of agronomic importance is selected from the group consisting of root length, rate of germination, dry shoot biomass and yield.
96. Synthetic composition according to any one of claims 90-93, characterized by the fact that the plant element is of the genus Oryza and the enhanced trait of agronomic importance is dry root biomass.
97. Synthetic composition characterized by the fact that it comprises a plant element and an endophyte organism arranged in a heterologous manner, in which the endophyte organism is a member of the genus Pestalotiopsis and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 44-48, in which the said synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism.
98. Synthetic composition according to claim 97, characterized by the fact that the endophyte organism comprises polynucleotide sequences at least 97% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 44-48.
99. Synthetic composition according to claim 97, characterized by the fact that the endophyte organism comprises polynucleotide sequences at least 97% identical to at least three sequences selected from the group consisting of SEQ ID NOs: 44-48.
100. Synthetic composition according to claim 97, characterized by the fact that the endophyte organism comprises polynucleotide sequences at least 97% identical to at least four sequences selected from the group consisting of SEQ ID NOs: 44-48.
101. Synthetic composition according to claim 97, characterized by the fact that the endophyte organism comprises polynucleotide sequences at least 97% identical to at least five sequences selected from the group consisting of SEQ ID NOs: 44-48.
102. Synthetic composition according to any one of claims 97-101, characterized by the fact that the endophyte organism belongs to the Pestalotiopsis neglecta taxonomy.
103. Synthetic composition according to any one of claims 97-101, characterized by the fact that the plant element is of the genus Triticum and the enhanced trait of agronomic importance is selected from the group consisting of the germination rate and biomass of dry sprout.
104. Synthetic composition characterized by the fact that it comprises a plant element and an endophyte organism arranged in a heterologous manner, in which the endophyte organism is a member of the genus Enterobacter and comprises at least one polynucleotide sequence at least 97% identical to a polynucleotide sequence selected from the group consisting of SEQ ID NOs: 49-51, in which the said synthetic composition is capable of providing an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism.
105. Synthetic composition according to claim 104, characterized by the fact that the endophyte organism comprises polynucleotide sequences at least 97% identical to at least two sequences selected from the group consisting of SEQ ID NOs: 49-51.
106. Synthetic composition according to claim 104, characterized by the fact that the endophyte organism comprises polynucleotide sequences at least 97% identical to at least three sequences selected from the group consisting of SEQ ID NOs: 49-51.
107. Synthetic composition characterized by the fact that it comprises a plant element and an endophyte organism arranged in a heterologous way, in which the endophyte organism is a member of the genus Enterobacter, as deposited under NRRL Culture Deposit No. B67465, in which the composition Synthetic is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the endophyte organism.
108. Synthetic composition characterized by the fact that a plant element and an endophyte organism arranged in a heterologous manner, in which the endophyte organism is a modified endophyte organism of the genus Enterobacter derived from the endophyte organism, as deposited under NRRL Deposit and Culture No. B67465 , in which the synthetic composition is able to provide an improved trait of agronomic importance compared to an element of the reference plant that does not additionally comprise the modified endophyte organism.
109. Synthetic composition according to any one of claims 104-108, characterized by the fact that said endophyte organism belongs to the taxonomy Enterobacter cowanii.
110. Synthetic composition according to any one of claims 104-108, characterized by the fact that the plant element is of the genus Oryza and the enhanced trait of agronomic importance is dry root biomass.
111. Synthetic composition according to any one of claims 68, 70, 71, 76, 81, 82, 87, 90, 97, 104, 107 or 108, characterized by the fact that said improved trait of agronomic importance is conferred under normal irrigation conditions.
112. Synthetic composition according to any one of claims 68, 70, 71, 76, 81, 82, 87, 90, 97, 104, 107 or 108, characterized by the fact that the plant element is a seed.
113. Synthetic composition according to claim 112, characterized by the fact that the seed is modified.
114. Synthetic composition according to any one of claims 68, 70, 71, 76, 81, 82, 87, 90, 97, 104, 107 or 108, characterized by the fact that it is placed on a substrate that promotes growth of the plants.
115. Synthetic composition according to claim 114, characterized by the fact that the substrate that promotes plant growth is the soil.
116. Plurality of synthetic compositions according to claim 115, characterized by the fact that a plurality of said plant elements are placed in the soil in rows, with substantially equal spacing between each seed within each row.
117. Synthetic composition according to any one of claims 68, 70, 71, 76, 81, 82, 87, 90, 97, 104, 107 or 108, characterized in that it comprises a formulation comprising one or more of following: stabilizer, preservative, carrier, surfactant, anti-complex agent or any combination of these.
118. Synthetic composition according to any one of claims 68, 70, 71, 76, 81, 82, 87, 90, 97, 104, 107 or 108, characterized by the fact that it comprises a formulation comprising one or more of following: fungicide, nematicide, bactericide, insecticide or herbicide.
119. Synthetic composition according to any one of claims 68, 70, 71, 76, 81, 82, 87, 90, 97, 104, 107 or 108, characterized by the fact that said compositions are confined to a selected object of the group consisting of: bottle, flask, ampoule, package, container, bag, box, can, envelope, cardboard box, container, silo, shipping container, body floor or crate.
120. Synthetic composition according to any one of claims 68, 70, 71, 76, 81, 82, 87, 90, 97, 104, 107 or 108, characterized by the fact that the synthetic combinations are shelf stable.
121. Synthetic composition according to any one of claims 68, 69, 76, 77, 78, 79, 80, 87, 90, 91, 92, 93, 97, 98, 99, 100, 101, 104, 105 or 106, characterized by the fact that the percentage of identity is at least 98%.
122. Synthetic composition according to any one of claims 68, 69, 76, 77, 78, 79, 80, 87, 90, 91, 92, 93, 97, 98, 99, 100, 101, 104, 105 or 106, characterized by the fact that the identity percentage is at least 99%.
123. Synthetic composition according to any one of claims 68, 69, 76, 77, 78, 79, 80, 87, 90, 91, 92, 93, 97, 98, 99, 100, 101, 104, 105 or 106, characterized by the fact that the identity percentage is 100%.
124. Synthetic composition according to any one of claims 68, 69, 76, 77, 78, 79, 80, 87, 90, 91, 92, 93, 97, 98, 99, 100, 101, 104, 105 or 106, characterized by the fact that the percentage of identity is determined over an alignment region of at least 100 nucleotides.
125. Synthetic composition according to any one of claims 68, 69, 76, 77, 78, 79, 80, 87, 90, 91, 92, 93, 97, 98, 99, 100, 101, 104, 105 or 106, characterized by the fact that the percentage of identity is determined over an alignment region of at least 200 nucleotides.
126. Synthetic composition according to any one of claims 68, 69, 76, 77, 78, 79, 80, 87, 90, 91, 92, 93, 97, 98, 99, 100, 101, 104, 105 or 106, characterized by the fact that the percentage of identity is determined over an alignment region of at least 300 nucleotides.
127. Synthetic composition according to any one of claims 68, 69, 76, 77, 78, 79, 80, 87, 90, 91, 92, 93, 97, 98, 99, 100, 101, 104, 105 or 106, characterized by the fact that the percentage of identity is determined over an alignment region of at least 400 nucleotides.
128. Synthetic composition according to any one of claims 68, 69, 76, 77, 78, 79, 80, 87, 90, 91, 92, 93, 97, 98, 99, 100, 101, 104, 105 or 106, characterized by the fact that the percentage of identity is determined over an alignment region of at least 500 nucleotides.
FIGURE 1 200-. 2 : . o = js .. ”o. O ". the 150 "ay Y SN sz.” .. and this is it.
DA o o ”E a. th S. "*. fe.“ .. v *, O. * 2 50-2.: É *: = ê o;; $ s S € E 8 T S S ú 2 - = =
FIGURE 2 2 8 300. thread
The 8. R * the E 200. The . 8:. NS ”2 o E 2º 14 + [A vo 2 ”V - A sp o and go in a, PAR nd a le e e o a SS Dre lo to A fo DE RO o da Ml and o FE E AS AO: o ..% Pa -. > ”2 a * v., <2 º = 2 co x Is the T Ss and co the Õ Õ = =

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法律状态:
2021-11-03| B350| Update of information on the portal [chapter 15.35 patent gazette]|

优先权:

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申请号 | 申请日 | 专利标题

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US201762465818P| true| 2017-03-01|2017-03-01|

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US62/465,818|2017-03-01|

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