• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    A rhizome plant glucose sensing sensor comprises a substrate (10), an electrode module (20), a hydrophobic insulating layer (30) and a cover (40). The electrode module (20) includes a measurement portion (21) and a connecting section (22) connected thereto. The hydrophobic insulating layer (30) covers the measuring portion (21) and the connecting section (22) and includes an opening (31). The cover (40) disposed on the opening (31) forms a sample channel (50) between the cover (40) and the substrate (10). The measuring portion (21) is arranged in the sample channel (50). The electrode module (20) includes a working electrode (24) and a reference electrode (25). The measuring portion of the working electrode (24) has a first exposed region (S1) in the sample channel (50). The present invention can detect glucose concentrations of rhizome plants.
    公开号:BE1024080B1
    申请号:E2015/0186
    申请日:2015-07-22
    公开日:2017-11-13
    发明作者:Yen-Yuan Shen；Chi-Yang Peng；Tzer-Ming Chen
    申请人:Eumed Biotechnology Co., Ltd.；
    IPC主号:
    专利说明:

    SENSOR FOR DETECTING GLUCOSE FROM RHIZOME PLANTS
    The present invention relates to a glucose sensor, in particular a rhizome plant glucose sensor.
    With the advancement of medical science and the development of the biomedical industry, the controls for medical examination, monitoring and administration, which could only be carried out in hospital so far, can now also be carried out at home. For example, miniature glucose sensors are widely used today to detect glucose concentration for the treatment and prevention of diabetes mellitus, whereby users (such as patients with diabetes mellitus) can experience their physiological states of adequate and immediate way in everyday life.
    U.S. Patent Publication No. US20030203498 discloses a system for detecting glucose concentrations in blood samples, which comprises a test strip and a measuring device. The test strip includes a sample chamber, a working electrode, a reference electrode, a plurality of sensor-fill electrodes, and an auto-activation lead. A reagent layer is disposed in the sample chamber. While the test strip is inserted into the sample chamber, the auto-activation lead activates the meter to initiate a detection process. The meter uses the working electrode and the reference electrode to initially detect the blood sample in the sample chamber. Then, the meter calculates the glucose level based on the measured current and the calibration data saved in a removable data storage device associated with the test strip.
    However, the dipstick of the aforementioned miniature glucose sensors can only detect the glucose concentration of blood samples. In other words, it can not detect glucose concentration outside the range of distribution of glucose concentration in ordinary blood samples. A non-blood sample, such as a juice sample from a rhizome plant, normally has a trace amount of glucose that is less than the glucose concentration of a regular blood sample. As a result, conventional miniature glucose sensors can not detect the glucose concentrations of juice samples and have limited application. The main objective of the present invention is to solve the problem that the conventional glucose sensor can not test the glucose concentrations of rhizome plant juices but can only test the glucose concentrations of blood samples.
    To achieve the above purpose, the present invention provides a rhizome plant glucose sensor which comprises a substrate, an electrode module, a hydrophobic insulating layer, and a cover. The electrode module is arranged on the substrate and includes a measurement portion and a connection section connected to the measurement portion. The hydrophobic insulating layer is disposed on the substrate to cover the measurement portion and includes an opening revealing the measurement portion. The cover is disposed on the opening to form a sample channel between the substrate and the cover. The sample channel includes a sample inlet formed on a circumference of the substrate and a blocking end opposite the sample inlet and blocked by the hydrophobic insulating layer. The measuring part is arranged between the sample inlet and the locking end.
    The electrode module includes a working electrode and a reference electrode. The measuring portion of the working electrode has a first exposed region having a surface of 4-6 mm 2 in the sample channel.
    In the present invention, the first exposed region of the measuring portion of the working electrode is designed to have an area of 4-6 mm 2. Once the sample of a juice sample from a rhizome plant is injected into the sample channel, the first exposed region is large enough to contact a sufficient amount of the trace glucose. in the juice sample to detect its glucose concentration. Thus, the present invention can detect the glucose concentration of a juice sample of a rhizome plant substantially.
    Fig. 1 is a perspective view schematically showing a rhizome plant glucose sensor according to an embodiment of the present invention; Fig. 2 is an exploded view schematically showing a rhizome plant glucose sensor according to an embodiment of the present invention; and Fig. 3 is a top view schematically showing a rhizome plant glucose sensor according to one embodiment of the present invention.
    We will hereinafter describe the technical content of the present invention in detail with reference to the drawings.
    Referring to FIGS. 1-3, which are respectively a perspective view, an exploded view, and a top view schematically showing a rhizome plant glucose sensor according to one embodiment of the present invention. The rhizome plant glucose sensing sensor of the present invention is used to detect glucose concentrations of rhizome plants, particularly for detecting glucose concentrations in rhizome plant juice samples. The rhizome plant glucose sensing sensor of the present invention comprises a substrate 10, an electrode module 20, a hydrophobic insulating layer 30 and a cover 40. The substrate 10 is of the strip type and has a circumference 11. circumference 11 includes two long edges 111 and two short edges 112. The long edges 111 are parallel. The short edges 112 are also parallel and each couple to the two long edges 111. The electrode module 20 is arranged on the substrate 10 and includes a measuring portion 21, a connecting section 22 and a signal reading element 23 The measuring part 21 and the signal reading element 23 are respectively connected to both ends of the connection section 22. The electrode module 20 uses the measurement part 21 to carry out the measurement. The signal reading element 23 is electrically connected to a measuring instrument (not shown in the drawings), which analyzes the signals detected by the measuring part 21. The electrode module 20 includes a working electrode 24 and a Reference electrode 25 arranged opposite the working electrode 24. Auxiliary electrodes 26 are coated on the connecting section 22 and the signal reading element 23 to improve electrical conduction. In one embodiment, the electrode module 20 is made of an electrically conductive polymer material, and the auxiliary electrodes 26 are made of silver paste.
    The hydrophobic insulating layer 30 is made of an ordinary hydrophobic material, such as polypropylene. The hydrophobic material may be in the form of a coating material, a laminated plate or a gel. The hydrophobic insulating layer 30 is disposed on the substrate 10 to cover the measuring portion 21 and the connecting section 22 of the electrode module 20 on the substrate 10. The hydrophobic insulating layer 30 has an opening 31 revealing the measuring portion 21 In one embodiment, the opening 31 extends towards one of the two long edges 111 of the substrate 10.
    The cover 40 is disposed on the aperture 31 to form a sample channel 50 between the cover 40 and the substrate 10. The sample channel 50 includes a sample inlet 51 and a locking end 52. Like the opening 31, the sample inlet 51 also extends to one of the two long edges 111 of the substrate 10. The locking end 52 is opposite to the sample inlet 51 and blocked by the hydrophobic insulating layer 30. The measuring part 21 is arranged between the sample inlet 51 and the locking end 52.
    The cover 40 includes a transparent portion 41, two raised regions 42 and a hydrophilic surface layer 43. The transparent portion 41 is arranged with respect to the opening 31 and covers the opening 31 to form the sample channel 50. The two raised regions 42 are respectively arranged on both sides of the opening 31 and connected to the hydrophobic insulating layer 30. The thickness of the raised regions 42 is greater than the thickness of the transparent portion 41. Thus, the transparent portion 41 will not come into contact with the hydrophobic insulating layer 30, and an aeration opening 60 is formed between the cover 40 and the locking end 52. The hydrophilic surface layer 43 is arranged on a surface of the transparent portion 41, which faces the sample channel 50. In some embodiments, the hydrophilic surface layer 43 is formed by coating the transparent portion 41 with a hydrophilic material, such as a material selected from a group consisting of ethyl cellulose, methyl cellulose, hydroxypropyl cellulose, cellulose acetate, nitrocellulose, polyvinyl pyrrolidone, polysulfone, polyvinylidene fluoride, polyamide and polyimide. In some embodiments, the cover 40 is directly made of a material selected from the above-mentioned group, whereby the hydrophilic surface layer 43 is unnecessary in these embodiments.
    In the embodiment shown in the accompanying drawings, the rhizome plant glucose sensing sensor of the present invention further comprises a reaction agent film 70 arranged in the sample channel 50 and connected to the electrode. The reaction agent film 70 includes a plurality of chemical agents, such as an oxidation reducing agent, a mediator, a buffer salt, and a surfactant. In one embodiment, the reaction agent film 70 includes FAD-conjugated glucose dehydrogenase and potassium hexacyanoferrate to implement glucose detection.
    In order to allow the liquid sample to react with the working electrode 24 and the reference electrode 25, the measuring portion 21 of the working electrode 24 has a first exposed region Si having a surface of 4-6. mm2 in the sample channel 50. In one embodiment, the measuring portion 21 of the working electrode 24 has a first rectangular exposed region S1 having first long edges XI each having a length of 2.3-2 , 7 mm and first short edges Y1 each having a length of 1.8-2.2 mm. The measuring portion 21 of the reference electrode 25 has a second exposed region S2 having an area of 2.5-4.5 mm 2 in the sample channel 50. In one embodiment, the measuring portion 21 of the reference electrode 25 has a second rectangular exposed region S2 having second long edges X2 each having a length of 2.3-2.7 mm and second short edges Y2 each having a length of 1.1-1.6 mm. Thus, a contact surface, which is sufficient to detect the glucose concentration of the liquid sample, exists between the liquid sample and the measuring portion 21 of the working electrode 24 and the reference electrode 25.
    Upon application, the user drops a liquid sample drop into the sample inlet 51. Because of the aeration aperture 60 and the hydrophilic surface layer 43, the liquid sample can flowing rapidly in the sample channel 50. Due to the hydrophobic insulating layer 30, the liquid sample is diffuse with difficulty on both sides of the sample channel 50 but manages to move the measurement part 21 almost completely. Accordingly, there is a sufficient contact area of the liquid sample, the reaction agent film 70 and the measuring portion 21 of the working electrode 24 and the measuring portion 21 of the working electrode 24. reference electrode 25 for detecting the glucose concentration of the liquid sample.
    In conclusion, the rhizome plant glucose sensor of the present invention is characterized in that the measuring portions of the working electrode and the reference electrode respectively have a first exposed region and a second exposed region. , which are in contact with the liquid sample injected into the sample channel to form reaction zones, and in that the reaction zones are sufficiently large to promote the sensitivity to a level required by a detection standard of a range of the glucose concentration of a juice sample of a rhizome plant. Therefore, the present invention has utility, novelty and non-obviousness and meets the requirement of patenting. As a result, the inventors file a patent application. It would be appreciated if the patent is approved quickly.
    The present invention has been demonstrated in detail with the embodiments described above. However, these embodiments serve only to illustrate the present invention and not to limit the scope thereof. Any equivalent change or variation consistent with the spirit, feature, or claim of the present invention will also be included in its scope.
    权利要求:
    Claims (9)
    [1]
    A rhizome plant glucose sensing sensor, comprising a substrate (10) and an electrode module (20) arranged on the substrate (10), wherein the electrode module (20) includes a measuring portion (21) and a connecting section (22) connected to the measuring part (21); a hydrophobic insulating layer (30) disposed on the substrate (10) for covering the measuring portion (21) and the connecting section (22) and including an opening (31) revealing the measuring portion (21); and a cover (40) disposed on the opening (31) for forming a sample channel (50) between the cover (40) and the substrate (10), the sample channel (50) including an input of sample (51) formed on a circumference (11) of the substrate (10) and a locking end (52) opposite the sample inlet (51) and blocked by the hydrophobic insulating layer (30), in wherein the measuring portion (21) is arranged between the sample inlet (51) and the locking end (52), and wherein the electrode module (20) includes a working electrode (24) and a reference electrode (25) opposite the working electrode (24), and wherein the measuring portion (21) of the working electrode (24) has a first exposed region (SI) having a 4-6 mm2 area in the sample channel (50).
    [2]
    The rhizome plant glucose sensing sensor according to claim 1, wherein the first exposed region (SI) of the measuring portion (21) of the working electrode (24) is formed into a rectangular shape having first long edges (XI) each having a length of 2.3-2.7 mm and first short edges (Y1) each having a length of 1.8-2.2 mm.
    [3]
    The rhizome plant glucose sensing sensor according to claim 1, wherein the measuring portion (21) of the reference electrode (25) has a second exposed region (S2) having a surface area of 2.5. 4.5 mm2 in the sample channel (50).
    [4]
    The rhizome plant glucose sensing sensor according to claim 3, wherein the second exposed region (S2) of the measuring portion (21) of the reference electrode (25) is formed into a rectangular shape having second long edges (X2) each having a length of 2.3-2.7 mm and second short edges (Y2) each having a length of 1.1-1.6 mm.
    [5]
    The rhizome plant glucose sensing sensor according to claim 1, wherein the substrate (10) is strip-like and has a circumference (11); the circumference (11) includes two parallel long edges (111) and two short edges (112) each coupling to the two long edges (111); the sample inlet (51) is positioned on one of the two long edges (111).
    [6]
    The rhizome plant glucose sensing sensor of claim 1, wherein the cache (40) includes a transparent portion (41) arranged with respect to the sample channel (50).
    [7]
    The rhizome plant glucose sensing sensor according to claim 6, wherein the cache includes a hydrophilic surface layer (43) arranged on a surface of the transparent portion (41) facing the sample channel (50). ).
    [8]
    The rhizome plant glucose sensing sensor of claim 1 further comprising a reaction agent film (70) arranged in the sample channel (50) and connected to the working electrode (24) and to the reference electrode (25).
    [9]
    The rhizome plant glucose sensing sensor of claim 8, wherein the reaction agent film (70) includes FAD-conjugated glucose dehydrogenase and potassium hexacyanoferrate.
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    同族专利:
    公开号 | 公开日
    BE1024080A1|2017-11-10|
    CN204731190U|2015-10-28|
    TWM493052U|2015-01-01|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    GB2530542A|2014-09-25|2016-03-30|Eumed Biotechnology Co Ltd|Sensor for detecting glucose of rhizome plants|
    US9823242B2|2015-08-07|2017-11-21|Apex Biotechnology Corp.|Sensor strip and manufacture method thereof and system thereof|
    法律状态:
    2018-02-08| FG| Patent granted|Effective date: 20171113 |
    2018-05-03| MM| Lapsed because of non-payment of the annual fee|Effective date: 20170731 |
    优先权:
    申请号 | 申请日 | 专利标题
    TW103213060U|TWM493052U|2014-07-22|2014-07-22|Glucose sensor for detecting rhizome plant|
    TW103213060|2014-07-22|
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