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    • 专利摘要:
    The invention relates to commercially viable methods for producing biologically active vitamin K dependent proteins, particularly Factor IX. Factor IX is produced at a level of at least about 15 mg/L and is at least 25% biologically active. The method relies upon co-expression of one or more of paired basic amino acid converting enzyme (PACE), vitamin K dependent epoxide reductase (VKOR) and vitamin K dependent y-glutamyl carboxylase (VKGC) at a preferred ratio so that the vitamin K dependent protein is efficiently produced and processed by a recombinant cell.
    公开号:AU2013211534A1
    申请号:U2013211534
    申请日:2013-08-02
    公开日:2013-08-22
    发明作者:William N. Drohan；Michael J. Griffith；Darrell W. Stafford；John R. Taylor
    申请人:Cangene Corp；University of North Carolina at Chapel Hill；University of North Carolina System；
    IPC主号:C12N15-57
    专利说明:
    H:sog1nInvovnNRPOblDCSCG5363H21_LDOC- [A)8/2013 METHOD OF PRODUCING BIOLOGICALLY ACTIVE VITAMIN K DEPENDENT PROTEINS BY RECOMBINANT METHODS Related Applications [0001] This is a divisional of Australian Patent Application No. 2006331501, the entire contents of which are incorporated herein by reference. [0001a] This application claims priority to U.S. provisional application No. 60/752,642, filed December 21, 2005, which is incorporated herein by reference. BACKGROUND OF THE INVENTION Field of the Invention 100021 Embodiments of the invention relate generally to production of recombinant vitamin K dependent proteins, particularly Factor IX, which are fully functional by co-expression of one or more proteins involved in the processing of the vitamin K dependent proteins. These processing proteins include paired basic amino acid converting enzyme (PACE), vitamin K dependent epoxide reductase (VKOR) and vitamin K dependent y-glutamyl carboxylase (VKGC). Additionally, the propeptide of the vitamin K dependent protein may be modified to improve y-carboxylation. Description of the Related Art [0003] Bleeding disorders can result from a deficiency in the functional levels of one or more of the blood proteins, collectively known as blood coagulation factors, that are required for normal hemostasis, i.e. blood coagulation. The severity of a given bleeding disorder is dependent on the blood level of functional coagulation factors. Mild bleeding disorders are generally observed when the functional level of a given coagulation factor reaches about 5% of normal, but if the functional level falls below 1%, severe bleeding is likely to occur with any injury to the vasculature. [0004] Medical experience has shown that essentially normal hemostasis can be temporarily restored by intravenous infusion of biological preparations containing one or more of the blood coagulation factors. So-called replacement therapy, whereby a biological preparation containing the deficient blood coagulation factor is infused when bleeding occurs (on demand) or to preventing bleeding (prophylactically), has been shown to be effective in managing patients with a wide variety of bleeding disorders. In general, for replacement - 1 - WO 2007/075976 PCT/US2006/048954 therapy to be effective, intravenous infusions of the missing coagulation factor are targeted to achieve levels that are well above 5% of normal over a two- to three-day period. [0005] Historically, patients who suffer from hemophilia, a genetically acquired bleeding disorder that results from a deficiency in either 6 tood coagulation Factor VIII (hemophilia A) or Factor IX (hemophilia B), were successfully treated by periodic infusion of whole blood or blood plasma fractions of varying degrees of purity. [0006] More recently, with the advent of biotechnology, biologically active preparations of synthetic (recombinant) blood coagulation factors have become commercially available for treatment of blood coagulation disorders. . Recombinant blood coagulation proteins are essentially free of the risks of human pathogen contamination-that continue to be a concern that is associated with even highpurity commercial preparations that are derived from human blood. [0007] Adequate treatment of bleeding disorders is largely' limited to the economically-developed regions of the world In the case of hemophilia it is estimated that over 75% of the patient population worldwide receives inadequate or, worse, no treatment of their disease. For many regions of the world, the cost of safe and effective commercial preparations of coagulation factors is prohibitive for routine management of bleeding disorders and, in some cases, only emergency treatment with donated products is available. [00081 In regions of the world where adequate treatment of bleeding disorders is potentially'available, the cost is very high and patients are almost always dependent on third party payors, e.g. health insurance or government subsidized programs, to acquire the commercial products needed. On average, hemophilia treatment in the United States is estimated to cost about $50,000 per patient per year fbr the commercial product required for routine, on-demand, care. However, this cost could be much higher insofar as the Medical and Scientific Advisory Committee for the National Hemophilia Foundation has recommended that patients should receive prophylactic treatment which, in the case of an adult hemophiliac, could drive the annual cost to well over $250,000 per year. Given that life-time insurance caps of about $1 million are generally associated with most policies in the United States, hemophiliacs are severely constrained in terms of the amount of commercial -2i WO 2007/075976 PCT/US2006048954 product that they can afford for care which, at the least, affects their quality of life during adulthood and, at the worst, raises the risk of life-threatening bleeding. [0009] For the past 25 years or so, biotechnology has offered the promise of producing low cost biopharmaceutical products. Unfortunately, this promise has not been met due in major part to the inherent complexity of naturally occurring biological molecules and a variety of limitations associated with the synthesis of their recombinant protein counterparts in genetically engineered cells. Regardless of the cell type, e.g. animal, bacteria, yeast, insect, plant, etc., that is chosen for synthesis, proteins must achieve certain minimal structural properties fbr safe and effective therapeutic use. In some cases, recombinant proteins must simply fold correctly after synthesis to attain the three-dimensional structure required for proper function. In other cases, recombinant proteins must undergo extensive, enzyme directed, post-translational modification after the core protein has been 'ynthesized within the cell. Deficiencies-in any one of a number of intracellular enzymatic activities can result in the formation of a large percentage bf non-functional protein and limit the usefulness of a genetically engineered cell system for the economical production of a biopharmaceutical product intended for commercial use. [0010] Several of the proteins required for normal blood coagulation are very complex in terms of having multiple structural domains each being associated with a very specific functional property that is essential for the overall effectiveness of the protein in controlling hemostasis and/or preventing thrombosis. 1I particular, the so-called "vitamin K dependent" blood coagulation proteins, e.g. Factors II, VII, IX, X, Protein C and Protein S, are very complex proteins and must undergo extensive post-translational modification for normal function. Achieving high levels of functional vitamin K-dependent proteins by recombinant technology has been limited by the structural complexity of these proteins and the inability to create genetically engineered cell systems that overcome the inherent deficiencies in the enzymatic activities required for efficient and complete post-translational modification to occur. Problem to be solved [0011] The first synthetic vitamin K-dependent blood coagulation protein to become commercially available was -Factor IX which is still manufactured today from -3- WO 2007/075976 PCT/US2006/048954 genetically eftgineered Chinese Hamster Ovary (CHO) cells ( BeneFix, Coagulation Factor IX (Recombinant) Directional Insert, Weyth Pharmaceuticals, Inc. Philadelphia, PA 19101 CI 8020-3 W10483C007, Rev 10/05). Although recombinant Factor IX can be produced using CHO cells, it is not optimal as a treatment for Hemophilia B because it has not been properly processed and consequently its bioavailability to patients is variable. While reasonable levels of recombinant Factor IX protein can be expressed by genetically engineered CHO cells, e.g. up to 188 mg/L, the levels of fully functional Factor IX that are produced are on the order of only 0.5 mg/L due to the limited ability of the CHO cells to fully gamma-carboxylate the first 12 glutamic acid residues in the amino terminal region of the protein referred to as the gla domain. In addition to this deficiency in the post-translational modification of Factor IX, subsequent work demonstrated that pro-Factor IX, a form of Factor IX that contains a propeptide domain that is required for the efficient intracellular gamma-carboxylation of the protein, is not processed completely prior to secretion from the CHO cell. As a consequence, it was found that well over half of the Factor IX secreted from genetically engineered CHO cells still contains the propeptide region and is non-functional (Bond, M., Jankowski, M., Patel, H., Karnik, S-, Strand, A., Xu, B., et al. (1998] Biochemical characterization of recombinant factor IX. Semin. Hematol. 35 [2 SuppL2], I1-17). [0012] The present application addresses a need for a method to produce vitamin K dependent proteins such as Factor IX which have been properly processed so that they are active and in sufficient yield for commercial production. To increase the availability of vitamin K-dependent blood coagulation proteins to meet the worldwide medical need for the treatment of bleeding disorders such as hemophilia B, improvements in the production of fully functional protein, Factor IX in this example, from genetically engineered cellsI are required. Specifically, identification and supplementation of deficiencies in the enzymatic activities required to obtain essentially complete post-translational modification are needed. SUMMARY OF THE INVENTION [00131 Embodiments of the invention are directed to methods of producing a recombinant biologically active vitamin K dependent protein product, which includes transfecting a mammalian cell with a gene encoding the vitamin K dependent protein operably linked to a promoter and at least one gene encoding a processing factor(s) operably -4- WO 2007/075976 PCT/US2006048954 linked to at least one promoter, either simultaneously or sequentially, and harvesting the vitamin K dependent protein product. Preferably, the cell produces biologically active vitamin K dependent protein product in an amount of at least about 15 mg/L [00141 In preferred embodiments, the vitamin K dependent protein product is Factor II, Factor VII, Factor IX, Factor X, Protein C or Protein S. More preferably, the vitamin K dependent protein is Factor IX or Factor VII. [0015] In preferred embodiments, the processing factor is a nucleic acid selected from paired basic amino acid converting enzyme (PACE), vitamin K dependent epoxide reductase (VKOR), vitamin K dependent y-glutamyl carboxylase (VKGQC) and combinations thereof operably linked to one or more promoter(s). Preferably, the one or more processing factor proteins is produced in an amount sufficient to facilitate the production of at least about 15 mg/L of the recombinant biologically active vitamin K dependent protein product. More preferably, the processing factor proteins include VKOR and VKGC. Preferably, at least one of the genes is overexpressed. More preferably, the overexpressed gene-is operably linked to a Chinese hamster elongation factor I-a (CHEFI) promoter. [0016] In preferred embodiments, at least about 75% of the glutamic acid residues within the gla-domain of the biologically active vitamin K dependent protein product are gamma carboxylated, {0017] In some preferred embodiments, the vitamin K dependent protein product has a deletion in a propeptide of the vitamin K dependent protein product (0018] In some preferred embodiments, the vitamin K dependent protein product includes a heterologous propeptide region which is from a vitamin K dependent protein which is different from the vitamin K dependent protein product. [0019] Preferably, at least 10% of the recombinant vitamin K dependent protein is biologically active. Marc preferably, at least 20% of the vitamin K dependent protein is biologically active. Yet more preferably, at least 50% of the vitamin K dependent protein is biologically active. Yet more preferably, at least 80% of the vitamin K dependent protein is biologically active. [biol ] In preferred embodiments, the mammalian cell is a CHO cell or a HEK 293 cell. -5- WO 2007/075976 PCT/US2006/04895 4 [00211 In preferred embodiments, the biologically active vitamin K dependent protein is produced in an amount of at least about 20 mg/L. More preferably, the biologically active vitamin K dependent protein is produced in an amount of at least about 30 mg/L. More preferably, the biologically active vitamin K dependent protein is produced in an amount of at least about 50 mg/L. [00221 In some preferred embodiments, transfection is sequential and transfecting the mammalian cell further includes selecting for cells Which express high levels of the vitamin K dependent protein product or the processing factor(s), cloning the selected cells, and amplifying the cloned cells. In some preferred embodiments, the transfecting steps with the gene(s) encoding the processing factor(s) are performed before the transfecting steps with the gene encoding the vitamin K dependent protein. In alternate preferred embodiments, the transfecting steps with the gene encoding the vitamin K dependent protein are performed before transfecting steps with the gene(s) encoding the processing factor(s). [0023] In preferred embodiments, the mammalian cell is selected for expression of endogenous levels of one or more processing factors before transfection. {0024] Embodiments of the invention are directed to a recombinant mammalian cell which includes a gene for a vitamin K dependent protein operably linked to a promoter and a gene for at least one processing factor operably linked to at least one promoter. The expression of the protein(s) encoded by the gene for at least one processing factor(s) in the cell-facilitates the production of biologically active vitamin K dependent protein in an amount of preferably at least about 15 mg/L. (0025] Preferably, the vitamin K dependent protein is Factor I, Factor VII, Factor IX, Factor X, Protein C or Protein S. More preferably, the vitamin K dependent protein is Factor IX or Factor VII. [0026J In preferred embodiments, the processing factor is- a gene which produces a processing gene product selected from PACE, VKOR, VKGC, and combinations thereof, operably linked to one or more promoter(s) for expression in said cell. More preferably, the processing factors include VKOR and VKGC. Preferably, the at least one processing gene products is expressed at a higher level than observed in normal, nontransfected cells of the -6- WO 2007/075976 PCTUS2006/048954 same line. More preferably, the overexpressed gene product is operably linked to a Chinese hamster elongation factor 1-a (CHEF 1) promoter. [00271 In preferred embodiments, the gene encoding the vitamin K dependent protein is modified to increase the percentage of glutamic acid residues which are carboxylated when compared to the percentage of carboxylated glutamnic acid residues present on vitamin K dependent protein produced from cells expressing a vitamin K dependent protein encoded by a gene encoding the unmodified yitamin K dependent protein. (00281 ' In some preferred embodiments, 'the modification includes a deletion in the propeptide region of the gene encoding the vitamin K dependent protein. [00291 In some preferred embodiments, the modification includes substitution of a propeptide region of the vitamin K dependent protein with a heterologous propeptide region from a heterologous vitaniin K dependent protein. [0030] Preferably, the recombinant mammalian cell is a CHO cell or HEK293 .cell. [0031] In some preferred embodiments, the cell used for transfection of a gene for a vitamin K dependent protein is preselected by selecting for variants of a specific tisstie culture cell line that contain naturally occurring modification enzymes capable of producing a vitamin K dependent protein composed of amino acids that are posttranslationally modified to contain at least 25% of the sulfation and at least 25% of the phosphorylation levels present in the corresponding plasma-derived vitamin K dependent protein. Preferably, the vitamin K dependent protein is Factor IX. [0032] In preferred embodiments, a recombinant Factor IX protein is produced by one or more of the method steps described herein. More preferably, the recombinant Factor IX protein produced by the methods described is included in a pharmaceutical composition. Some preferred embodiments are directed to a kit which includes the recombinant Factor IX protein produced according to the methods described herein. Preferably, the recombinant Factor IX protein is used in a method of treating hemophilia by administering an effective amount of the recombinant Factor IX protein to a patient in need thereof. -7- WO 2007/075976 PCT/US2006/048954 [00331 Preferred embodiments are directed to methods of producing recombinant biologically active vitamin K dependent protein products, by a process involving one or more of the following steps: (a) transfecting a mammalian cell with a gene encoding the vitamin K dependent protein operably linked to a promoter; -(b) selecting for cells which express high levels of the vitamin K dependent protein product; (c) transfecting the selected cells with one or more processing factor(s) operAbly linked to a promoter; (d) repeating step (b); (e) optionally, repeating steps (a) and/or (c) followed by (b); (f) cloning the selected cells; (g) amplifying the cloned cells; and (h) harvesting the product from the cloned cells in an amount of at least 15 mg/L recombinant biologically active vitamin K dependent protein. [00341 Further aspects, features and advantages of this invention will become apparent from the detailed description of the preferred embodiments which follow. Brief Description of the Drawing [0035] These and other feature of this invention will now be described with reference to the drawings of preferred embodiments which are intended to illustrate and not to limit the invention. [00361 The Figure shows the total amount of Factor IX produced per clone after transfection of a wild-type Factor IX gene into CHO cells. The Factor X gene was under the control of the CHEF-I promotor. Cells were allowed to grow in 5% serum for 14 days. The cell culture medium was harvested and the total amount of Factor IX antigen in pg per rnL was quantified by a Factor IX ELISA method. -8- WO 2007/075976 PCT/US2006/0489 5 4 DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENT [0037] While the described embodiment represents the preferred embodiment of -the present invention, it is to be understood that modifications will occur to those skilled in the art without departing from the spirit of the invention. The scope of the invention is therefore to be determined solely by the appended claims. [00381 . Preferred embodiments of the invention are directed to methods for. creating a genetically engineered cell that produces a high percentage of biologically active vitamin K-dependent protein in quantities suitable for commercialization world wide. Embodiments of the invention are described with respect to production of Factor IX. However, the disclosed methods are applicable to all vitamin K dependent proteins. [00391 To produce low cost vitamin K-dependent protein biotherapeutics for commercial use on a worldwide basis, a genetically engineered cell must be created for production that (1) produces large quantities of the polypeptide chain that has the desired primary structure and (2) is capable of efficiently performing all' of the essential post translational modifications that are needed to produce a fully functional synthetic biopharmaceutical product. [0040] As used herein, the term "commercial use" means a Factor IX or other vitamin K dependent protein which, when produced from tissue culture cells, is at least 10% biologically active and is capable of production at a level of at least about 30 mg/L. [0041] As used herein, "biologically activity" is determined with reference to a Factor IX standard derived from human plasma, such as MONONINE@ (ZLB Behring). The biological activity of the Factor IX standard is taken to be 100%. Preferably, the Factor IX according to embodiments of the invention has at least 20% of the activity of the Factor IX standard, more prefembly at least 25% of the activity of the Factor IX standard, more preferably at least 30% of the activity of the Factor IX standard, more preferably at least 35% -of the activity of the Factor IX standard, more preferably at least 40% of the activity of the Factor DC standard, more preferably at least 45% of the activity of the Factor IX standard, More preferably at least 50% of the activity of the Factor IX standard, more preferably at least 55% of the activity of the Factor IX standard, more preferably at least 60% of the activity of the Factor IX standard, more preferably at least 65% of the activity of the Factor IX standard, -9- WO 2007/075976 PCT/US2006/048954 more preferably at least 70% of the activity of the Factor IX standard, more preferably at least 75% of the activity of the Factor IX standard, more preferably at least 80% of the activity of the Factor IX standard, more preferably at least 85% of the activity of the Factor IX standard, more preferably at least 90% of the activity.of the Factor IX standard. 100421 Vitamin K dependent proteins according to the invention are capable of production at a level of at least about 20 mg/L, preferably at least about 30 mg/L, more preferably at least about 40 mg/L, more preferably at least about 50 mg/L, yet more preferably at least about 60 mg/L, yet more preferably at least about 70 mg/L, yet more preferably at least about 80 mg/L, yet more preferably at least about 90 mg/L, yet more preferably at least about 100 mg/L, yet more preferably at least about 110 mg/L, yet more preferably at least about 120 mg/L, .yet more preferably at least about 130 mg/L, yet more preferably at least about 140 mg/L, yet more preferably at least about 150 mg/L, yet more preferably at least about 160 mg/L, yet more preferably at least about 170 mg/L, yet more preferably at least about 180 mg/L, yet more preferably at least about 190 mg/L, yet more preferably at least about 200 mg/L, yet -more preferably at least about 210 mg/L of biologically active vitamin K dependent protein. [00431 The term "processing factor" is a broad term which includes any protein, peptide, non-peptide cofactor, substrate or nucleic acid which promotes the formation of a functional vitamin K dependent protein. Examples of such processing factors include, but are not limited to, PACE, VKOR and VKGC. [0044] "Limit dilution cloning" has its usual and customary meaning and refers to a process of obtaining a monoclonal cell population starting from a polyclonal mass of cells. The starting (polyclonal) culture is serially diluted until a monoclonal culture is obtained. [00451 Genetics Institute has shown that the production of large quantities of vitamin K dependent proteins is possible in genetically engineered CHO cells (U.S. Patent No. 4,770,999), but the percentage of fully functional protein is very low. An object of the present invention is a genetically engineered CHO cell that produces large quantities of vitamin K-dependent proteins whereby the percentage of fully functional protein is adequate to produce a low cost biopharmaceutical product for commercial use on a worldwide basis. -10- WO 2007/075976 PCT/US2006/048954 [00461 Stafford (U.S. Patent No. 5,268,275) has shown that th-e production of a high percentage of gamma-carboxylated vitamin K dependent proteins is possible in genetically engineered HBK 293 cells that are created to co-express enzymes that enhance the carboxylation of vitamin K-dependent proteins, but the total amount of gamma-carboxylated protein that is produced is very low. An object of the present invention is a genetically engineered HEK 293, CHO or other cell that produces large quantities of vitamin K dependent proteins whereby the percentage of fully functional protein is adequate to produce a low cost biopharmaceutical product for commercial use on a worldwide basis. [0047] Many 'transfection methods to create genetically engineered cells that express large quantities of recombinant proteins are well known. Monoclonal antibodies, for example, are routinely manufactured from genetically engineered cells that express protein levels in excess of 1000 mg/L. The present invention is not dependent on any specific transfection method that might be used to create a genetically engineered cell. [0048] Many expression vectors can be used to create genetically engineered cells. Some expression vectors are- designed to express large quantities of recombinant proteins after amplification of transfected cells under a variety of conditions that favor selected, high expressing, cells. Some expression vectors are designed to express large quantities of recombinant proteins without the need for amplification under selection pressure. The present invention is not dependent on the use of any specific expression vector. [0049] To create a genetically engineered cell to produce large quantities of a given vitamin K-dependent protein, cells are transfected with an expression vector that contains the cDNA encoding the protein. The present invention requires that a transfected cell is created that is capable, under optimized growth conditions, of producing a minimum of 20 mg/L of the target vitamin K-dependent protein. Higher levels of production of the target vitamin K-dependent protein may be achieved and could be useful in the present invention. However, the optimum level of production of the target vitamin K-dependent protein is a level at or above 20 mg/L that can be obtained in a significantly increased functional form when the target protein is expressed with selected co-transfected enzymes that cause proper post-translational modification of the target protein to occur in a given cell system. -11- WO 2007/075976 PCT/US2006/048954 [0050] To create a genetically engineered cell that is capable of efficiently performing all of the essential post-translational modifications that are needed to produce a fully functional synthetic biopharmaceutical product, selected enzymes are co-transfected along with the vitamin K-dependent protein. Genetics Institute has shown that genetically engineered CHO cells that produce large quantities of vitamin K-dependent protein (Factor IX) have not been properly processed to remove the propeptide region prior to secretion. In this case, Genetics Institute has found that co-expression of an enzyme (PACE), known to remove the propeptide region from vitamin K-dependent proteins, substantially eliminates the deficiency in the intrinsic cellular levels of the enzyme. However, Genetics Institute has also shown that deficiencies in the intrinsic levels of other enzymes result in the majority of the vitamin K-dependent protein produced by genetically engineered CHO cells to be non functional due to the low percentage of post-translational gamma-carboxylation of the gla domain (Bond, vL, Jankowski, M., Patel, H., Kamik, S., Strand, A., Xu, 1B., et al. [1998] biochemical characterization of recombinant factor IX. Semin. Hematol. 35 {2 Suppl.2], I I 17). [0051] The method of the present invention involves the first selection of a cell that may be genetically engineered to produce large quantities of a vitamin K-dependent protein such as Factor IX. [0052] The cell may be selected from a variety of sources, but is otherwise a cell that may be transfected with an expression vector containing a nucleic acid, preferably a cDNA of a vitamin K-dependent protein. [0053] From a pool of transfected cells, clones are selected that produce quantities of the vitamin K-dependent protein over a range (Target Range) that extends from the highest level to the lowest level that is minimally acceptable for the production of a commercial product. Cell clones that produce quantities of the vitamin K-dependent protein within the Target Range may be combined to obtain a single pool or multiple sub-pools that divide the clones into populations of clones that produce high, medium or low levels of the vitamin K dependent protein within the Targdt Range. [0054] It is considered to be within the scope of the present invention that transfected cells that produce a vitamin K-dependent protein within the Target Range may be -12- WO 20071075976 PCT/US20061048954 analyzed to determine the extent to which fully functional protein is produced. Such analysis will provide insight into the specific enzyme deficiencies that limit the production of fully functional protein. Further, it is anticipated that analysis of sub-pools consisting of cell clones that produce high, medium, or low levels of the vitamin K-dependent protein within the Target Range will provide insight into the specific enzyme deficiencies that limit the production of fully functional protein at varying levels of production of the vitamin K dependent protein. Such analysis, whether done on a single pool of cell clones or on sub pools, might reveal the specific enzyme deficiencies that must be eliminated to produce fully functional protein. [00551 To eliminate the enzyme deficiencies within a pool of transfected clones that limits the production of fully functional vitamin K-dependent protein within the Target Range, embodiments of the present invention provide for the transfection of the pool of cells with an expression vector containing a nucleic acid, preferably a cDNA for a protein that, when expressed by a cell clone, will mitigate the enzyme deficiency in whole or in part. In preferred embodiments, it is further contemplated that more than one enzyme deficiency may be mitigated or that mitigation of a deficiency in post-translational modification of the vitamin K-dependent protein requires the presence of the activities of more than one enzyme or protein or other processing factor that may be provided in the method of the present invention by the simultaneous or subsequent (seqential) transfection of the cell clones with additional expression vectors containing cDNA for given proteins. [00561 In some embodiments, the host cell may first be transfected with gene(s) encoding one or more processing factors and subsequently transfected with a gene encoding a vitamin K dependent protein. In some embodiments, the host cell is first transfected with a gene encoding a vitamin K dependent protein and subsequently transfected with one or more processing factors. Optionally, the host cell may be transfected with the gene(s) for the processing factor(s) or with the gene for the vitamin K dependent protein that is the same-or substantially the same as an earlier transgene. After each round of transfection, clones are selected which express optimal levels of the transgene. [00571 In some preferred embodiments, one such protein would have the enzymatic activity of vitamin K epoxide reductase (VKOR). In some preferred -13- WO 2007/075976 PCT/US2006/048954 ) erhbodiments, another such enzyme would have the enzymatic activity of vitamin K dependent ganma-glutamyl carboxylase (VKGC). In some preferred embodiments, another such enzyme would have the enzymatic activity of paired amino acid cleaving enzyme, i.e. PACE or furin. (00581 It is the object of the present invention to provQide a method to identify the minimum protein transfection requirements to obtain a high percentage of fully functional vitamin K-dependent protein from a cell clone that produces the vitamin K-dependent protein in a quantity within the Target Range. (00591 In preferred embodiments of the present invention, pools of cell clones that produce a vitamin K-dependent protein within the Target Rarige are subsequently transfected to provide a specific protein or multiple proteins in various combinations. Transfected pools of cell clones are then analyzed to determine the relative percentages of fully functional vitamin K-dependent protein that are now produced by transfectant pools that co-express the various proteins. The transfectant pool that produces the highest percentage-of fully functional vitamin K-dependent protein with the minimum number of co-expressed proteins, is selected for subsequent'cloning. [00601 In preferred embodiments of the present invention, the selected transfectant pool is cloned to determine the optimal level of production of fully functional vitamin K-dependent protein that is attained by co-expression of additional protein(s). It is contemplated that higher percentages of fully functional vitamin K-dependent protein will be produced by cell-clones that produce lower total amounts of the vitamin K-dependent protein within the Target Range. In some embodiments, some cell clones may be superproducers of vitamin K dependent protein without significant improvements in post translational processing. Nevertheless, such superproducer lines produce usable amounts of functional protein as the overall production level is high. In preferred embodiments, the optimal level of production will be the highest level of functional vitamin K-dependent protein. [00611 The practice of the present invention employs, unless otherwise indicated, conventional techniques of molecular biology, microbiology, recombinant DNA, and immunology, which are within the skill of the art. Such techniques are explained fully in the literature. See, e.g., Sambrook, et al., "Molecular Cloning; A Laboratory Manual", 2nd ed -14- WO 20071075976 PCT/US2006/048954 (1989); "DNA Cloning", Vols. I and II (D. N Glover ed. 1985); "Oligonucleotide Synthesis" (M. J. Gait ed. 1984); "Nucleic Acid Hybridization" (B. D. Hames & S. J. Higgins eds. 1984); "Transcription and Translation" (B. D. Haines & S: J. Higgins eds. 1984); "Animal Cell Culture" (R. . Freshney ed. 1986); "Immobilized Cells and Enzymes" (IRL Press, 1986); B. Perbal, "A Practical Guide to Molecular Cloning" (1984); the series, Methods in Enzymology (Academic Press, inc.), particularly Vols. 154 and 155 (Wu and Grossman, and Wu, eds., respectively); "Gene Transfer Vectors for Manmnalian Cells" (J. H. Miller and M. P. Calos eds. 1987, Cold Spring Harbor Laboratory); " Immunochemical Methods in Cell and Molecular Biology", Mayer and Walker, eds. (Academic Press, London, 1987); Scopes, "Protein Purification: Principles and Practice", 2nd ed. 1987 (Springer-Verlag, N.Y.); and "Handbook of Experimental Immunology" Vols I-IV (D. M. Weir and C. C. Blackwell eds 1986). All patents, patent -applications, and publications cited in the background and specification are incorporated herein by reference. Modification of the propeptide [0062] In some embodiments, y-carboxylation is increased by replacing the native propeptide sequence with a propeptide sequence that has a lower affinity for the gamxma carboxylase as discussed in U.S. Application No. 2003/0220247, which is incorporated herein by reference. Useful propeptide sequences include altered forms of wild type sequences or propeptide sequences, or. combinations of the same, for heterologous vitamin K dependent proteins. The propeptide sequence i vitamin K-dependent proteins is the recognition element for the enzyme which directs ganuna carboxylation of the protein. Vitamin K-dependent proteins are not fully functional unless they comprise a high percentage of gamma carboxylated moieties. Thus, it is important when generating recombinant versions of these proteins that mechanisms be put in place to ensure full gamma carboxylation of the same. (0063 The sequence alignment of several propeptide sequences is shown in FIG. 3 of US. 2003/0220247. Thus, propeptides which are useful in the present invention are those which have the sequences shown in FIG. 3 wherein an 18 amino acid sequence of several useful propeptides is shown along with the relative affinities of these propeptides for gamma carboxylase. A low affinity propeptide may be generated by modifying any one of amino -15- WO 2007/075976 PCT/US2006/048954 Acids -9 or -13 on either prothrombin or protein C. Preferred modifications include the substitution of an Arg or a His residue at position -9 and the substitution of a Pro or i Ser residue at position -13. Other preferred chimeric proteins include a propeptide selected from the group consisting of altered Factor IX, Factor X, Factor VII, Protein S, Protein C and prothrombin, or an unaltered propeptide in combination with the mature vitamin K dependent protein which is not native to the chosen propeptide sequence. [0064] The term "fully gamma carboxylated protein" is used herein to refer to a protein wherein at least about 80% of the amino acids which should be gamma carboxylated are carboxylated. Preferably, at least about 85%, more preferably, at least about 90%, more preferably at least-about 95% and even more preferably, at least about 99% of the amino acids which should be gamma carboxylated are gamma carboxylated. Paired basic amino acid converting ez e (PACE) [0065] As used herein, the term "PACE" is an acronym for paired basic amino acid converting (or cleaving) enzyme. PACE, originally isolated from a human liver cell line, is a subtilisin-like endopeptidase, i.e., a propeptide-cleaving enzyme which exhibits specificity for cleavage at basic residues of a polypeptide, e.g., -Lys-Arg-, -Arg-Arg, or -Lys Lys-. PACE is stimulated by calcium ions; and inhibited by phenylmethyl sulfonyl fluoride (PMSF). A DNA sequence encoding PACE (or furin) appears in FIG. I [SEQ ID NO: 1] of U.S. Patent No. 5,460,950, which is incorporated herein by reference. The co-expression of PACE and a proprotein which requires processing for production of the mature protein results in high level expression of the mature protein. Additionally, co-expression of PACE with proteins requiring y-carboxylation -for biological activity pennits the expression of increased yields of functional, biologically active mature proteins in eukaryotic, preferably mammalian, cells. Vitamin K dependent epoxide reductase [0066J Vitamin K dependent epoxide reductase (VKOR) is important for vitamin K dependent proteins'because vitamin K is converted to vitamin K epoxide during reactions in which it is a cofactor. The amount of vitamin K in the human diet is limited Therefore, vitamin K epoxide must be converted back to vitamin K by VKOR to prevent depletion. Consequently, co-transfection with VKOR provides sufficient vitamin K for proper -16- WO 2007/075976 PCT/US2006/048954 ) functioning of the vitamin K dependent enzymes such as the vitamin K dependent y-glttamyl carboxylase (VKCG). Proper functioning of vitamin K dependent VKCG is essential for proper y-carboxylation of the gla-domain of vitamin K dependent coagulation factors. Vitamin K dependent gamma carboxylase [0067] Vitamin K dependent y-glutamyl carboxylase (VKGC) is an ER enzyme involved in the post-translation modification of vitamin K depeiideat proteins. VKGC incorporates CO2 into glutamic acid to modify multiple residues within the vitamin K dependent protein within about 40 residues of the propeptide. The loss of three carboxylations markedly decreases the activity of vitamin K-dependent proteins such as vitamin K dependent coagulation 'factors. The cDNA sequence for human vitamin K dependent y-glutamyl carboxylase is described by U.S. Patent No. 5,268,275, which is incorporated herein by reference. The sequence is provided in SEQ ID NO: 15 of U.S. Patent No. 5,268,275. Genetic Engineering Techniques [0068] The production of cloned genes, recombinant DNA, vectors, transformed host cells, proteins and protein fragments by genetic engineering is well known. See, e.g., U.S. Pat. No. 4,761,371 to Bell et al. at Col. 6 line 3 to Col. 9 line 65; U.S. Pat. No. 4,877,729 to Clark et al. at Col. 4 line 38 to Col. 7 line 6; U.S. Pat. No. 4,912,038 to Schilling at Col. 3 line 26 to Col. 14 line 12; and U.S. Pat. No. 4,879,224 to Wallner at Col. 6 line 8 to Col. 8 line 59. [0069) A vector is a replicable DNA construct. Vectors are used herein either to amplify DNA encoding Vitamin K Dependent Proteins and/or to express DNA which encodes Vitamin K Dependent Proteins. An expression vector is a replicable DNA construct in which a DNA sequence encoding a Vitamin K dependent protein is operably linked to suitable control sequences capable of effecting the expression of a Vitamin K dependent proteinin a suitable host. The need for such control sequences will vary depending upon the host selected and the transformation method chosen. Generally, control sequences include a transcriptional promoter, an optional operator sequence to control transcription, a sequence encoding suitable mRNA ribosomal binding sites, and sequences which control the termination of transcription and translation. -17- WO 2007/075976 PCT/US2006/048954 [00701 Amplification vectors do not require expression control domains. All that is needed is the ability to replicate in a host, usually conferred by an origin of replication, and a selection gene to facilitate recognition of transformants. [00711 Vectors comprise plasmids, viruses (e.g., adenovirus, cytornegalovirus)i phage, and integratable DNA fragments (i.e., fragments integratable into the host genome by recombination). The vector replicates and functions independently of the host genome, or may, in some instances, integrate into the genome itself. Expression vectors should contain a promoter and RNA binding sites which are operably linked to the gene to be expressed and are operible in the host organism. (0072) DNA regions are operably linked or operably associated when they are functionally related to each other. For example, a promoter is operably linked to a' coding sequence if it controls the transcription of the sequence; or a ribosome binding site is operably-linked to a coding sequence if it is positioned so as to permit translation. [0073] Transformed host cells are cells which have been transfoned or transfected with one or more Vitamin K dependent protein -vector(s) constructed using recombinant DNA techniques. Expression of multiple proteins [0074] Embodiments of the invention are directed to providing the cell with the necessary enzymes and cofactors to process -Vitamin K dependent proteins so that higher yields of biologically active Vitamin K dependent proteins are achieved. When adequate levels of fully functional Vitamin K dependent proteins are produced by a recombinant cell, lengthy purification steps designed to remove the useless, partially modified, or unmodified Vitamin K dependent protein from the desired product are avoided. This lowers the production cost and eliminates inactive material that may have undesirable side effects for the patient. (0075] In preferred embodiments, methods for producing Vitamin K dependent proteins by co-expression with PACE, VKGC and/or VKOR can include the following techniques. First, a single vector containing coding sequences for more than one protein such as PACE and a Vitamin K dependent protein can be inserted into a selected host cell. Alternatively, two or more separate vectors encoding a Vitamin K dependent protein plus one -18- WO 2007/075976 PCT/US2006/048954 or more other proteins, can be inserted into a host. Upon culturing under suitable conditions for the selected host cell, the two or more polypeptides are produced and interact to provide cleavage and modification of the proprotein into the mature protein. [0076] Another alternative is the use of two transfonned host cells wherein one host cell expresses the Vitamin K dependent protein and the other host cell expresses one or more of PACE, VKGC and/or VKQR which will be secreted into the medium. These host cells can be co-cultured under conditions which allow expression and secretion or release of the recombinant Vitamin K dependent protein and the co-expressed recombinant polypeptides, including cleavage into the mature form by the extracellular PACE and gamma carboxylation of N-terminal glutamates. In this method, it is preferred that the PACE polypeptide lacks the transmembrane domain so that it secretes into the medium. [00771 In some instances, it may be desirable to have a piprality of copies, two or more, of the gene expressing the Vitamin K dependent protein in relation to the other genes, or vice versa. This can be achieved in a variety of ways. For example, one may use separate vectors or plasmids, where the vector containing the Vitamin K dependent protein encoding polynucleotide has a higher copy number than the vector containing the other polynucleotide sequences, or vice versa. In this situation, it would be desirable to have different selectable markers on the two plasmids, so as to ensure the continued maintenance of the plasmids in the host. Alternatively, one or both genes could be integrated into the host genome, and one of the genes could be associated with an amplifying gene, (e.g., dhfr or one of the metallothionein genes). [00781 Alternatively, one could employ two transcriptional regulatory regions havirig different rates of transcriptional initiation, providing for the enhanced expression of either Vitamin K dependent protein or the expression of any of the other processing factor polypeptides, relative to Vitamin K dependent protein. As another alternative, one can use different promoters, where one promoter provides for a low level of constitutive expression of Vitamin K dependent protein, while the second promoter provides for a high level of induced expression of the other products. A wide variety of promoters are known for the selected host cells, and can be readily selected and employed in the invention by one of skill -19- WO 2007/075976 PCT/US2006/048954 in the art such as CMV, MMTV, SV 40 or SRu promoters which are well known manmnalian promoters. [00791 In a preferred embodiment, a promoter for the elongation factor -la from Chinese hamster is used (CHEF 1) to provide high level expression of a vitamin K dependent coagulation factor and/or processing factor(s). The CHEFi1 vector is used as described in Deer, et al.(2004) "High-level expression of proteins in manunalian cells using transcription regulatory sequences from the Chinese Hamster EF-la gene" Biotechnol. Prog. 20: 880-889 and in U.S. Patent No. 5,888,809 which -is incorporated herein by reference. The CHEFI vector utilizes the 5' and 3' flanking sequences from the Chinese .hamster EF-la. The CHEF promoter sequence includes approximately 3.7 kb DNA extending from a Spel restriction site to the initiating methionine (ATG) codon of the EF-la protein. The DNA -sequence is set forth in SEQ ID NO: I of U.S. Patent No. 5,888,809. [00801 Production of biologically active vitamin K dependent proteins such as Factor IX, are maximized by overexpression of one or more of PACE, VKOR, and/or VKGC and/or by modification of the gla region to maximize y-carboxylation. That is, rate limiting components are expressed in sufficient quantity so that the entire system operates to produce a commercially viable quantity of Vitamin K dependent protein. Host cells [00813 Suitable host cells include prokaryote, yeast or higher eukaryotic cells such as mammalian cells and insect cells. Cells derived from multicellular organisms are a particularly suitable host for recombinant Vitamin K Dependent protein synthesis, and mammalian cells are particularly preferred. Propagation of such cells in cell culture has become a routine procedure (Tissue Culture, Academic Press, Kruse and Patterson, editors (1973)). Examples of useful host cell lines are VERO and HeLa cells, Chinese hamster ovary (CHO) cell lines, and W1138, HEK 293, BHK, COS-7, CV, and MDCK cell lines. Expression vectors for such cells ordinarily include (if necessary) an origin of replication, a promoter located upstream from the DNA encoding vitamin K dependent protein(s) to be expressed and operatively associated therewith, along with a ribosome binding site, an RNA splice site (if intron-containing genomic DNA is used), a polyadenylation site, and a transcriptional termination sequence. In a preferred embodiment, expression is carried out in -2Q- WO 2007/075976 PCT/US2006/048954 Chinese Hamster Ovary (CHO) cells using the expression system of U.S. Patent No. 5,888,809, which is incorporated herein by reference. [0082] The transcriptional and translational control sequences in, expression vectors to be used in transforming vertebrate cells are often provided by viral sources. For example, commonly used promoters are derived from polyoma, Adenovirus 2, and Simian Virus 40 (SV40). See. e.g.. U.S. Pat. No. 4,599,308. [0083] An origin of replication may be provided either by construction of the vector to include an exogenous origin, such as may be derived from SV 40 or other viral (e.g. Polyoma, Adenovirus, VSV, or BPV) source, or may be provided by the host cell chromosomal replication mechanism. If the vector is integrated into the host cell chromosome, the latter is often sufficient. [00841 Rather than using vectors which contain viral origins of replication, one can transform mammalian cells by the method of cotransformation with a selectable marker and the DNA for the Vitamin K Dependent protein(s). Examples of suitable selectable markers are dihydrofolate reductase (DHFR) or thymidine kinase. This method is further described in U.S. Pat. No. 4,399,216 which is incorporated by reference. [0085] Other methods suitable for adaptation to the synthesis of Vitamin K Dependent protein(s) in recombinant vertebrate cell culture include those described in M-J. Gething et al., Nature 293, 620 (1981); N. Mantei et al., Nature 281, 40; A. Levinson et al., EPO Application Nos. 117,060A and 117,058A. [0086] Host cells such as insect dells (e.g., cultured Spodoptera frgiperda cells) and expression vectors such as the baculovirus expression vector (e.g., vectors derived from Autographa californica MNPV, Trichoplusia ni MNPV, Rachiplusia ou MNPV, or Galleria ou MNPV) may be employed in carrying out the present invention, as described in U.S. Pat. Nos. 4,745,051 and 4,879,236 to Smith et al. In general, a bacidovirus expression vector comprises a baculovirus genome containing the gene to be expressed inserted into the polyhedrin gene at a position ranging from the -polyhedrin transcriptioral start signal to the ATG start site and under the transcriptional control of a baculovirus polyhedrin promoter. [0087] Prokaryote host cells include gram negative or grdm positive organisms, for example Escherichia coli (E. coli) or Bacilli. Higher eukaryotic cells include established -21- WO 20071075976 PCT/U52006/048954 ) . cell lines of mammalian origin as described below. Exemplary host cells are E. coli W31 10 (ATCC 27,325), E. coli B, E. coli X1776 (ATCC 31,537), E. coli 294 (ATCC 31,446). A broad variety of suitable prokaryotic and microbial vectors are available. E. coli is typically transformedd using pBR322. Promoters most commonly used in recombinant microbial expression vectors include the betalactamase (penicillinase) and lactose promoter systems (Chang et al., Nature 275, 615 (1978); and Goeddel et al., Nature 281, 544 (1979)), a. tryptophan (trp) promoter system (Goeddel et al., Nucleic Acids Res. 8, 4057 (1980) and EPO App. Publ. No. 36,776) and the tac promoter (H. De Boer et al., Proc. Nati. Acad. Sci. USA 80, 21 (1983)). The promoter and Shine-Dalgarno sequence (for prokaryotic host expression) ate operably linked to the DNA encoding the Vitamin K Dependent protein(s), i.e., they are positioned so as to promote transcription of Vitamin K Dependent Protein(s) messenger RNA from the DNA. [00881 Eukaryotic microbes such as yeast cultures may also be transformed with Vitamin K Dependent Protein-encoding vectors. see, e.g., U.S. Pat. No. 4,745,057. Saccharomyces cerevisiae is the most commonly used among lower eukaryotic host microorganisms, although a number of other strains are commonly available. Yeast vectors may contain an origin of replication from the 2 micron yeast plasmid or an autonomously replicating sequence (ARS), a promoter, DNA encoding one or more Vitamin K Dependent ptoteins, sequences for polyadenylation and transcription termination, and a selection gene. An exemplary plasmid is YRp7, (Stinchcomb et al., Nature 282, 39 (1979); Kingsman et al., Gene 7, 141 (1979); Tschemper et al., Gene 10, 157 (1980)). Suitable promoting sequences in yeast vectors include the promoters for metallothionein, 3-phosphoglycerate kinase (Hitzeman et al., 1. Biol. Chem. 255, 2073 (1980) or other glycolytic enzymes (Hess et al., J. Adv. Enzyme Reg. 7, 149 (1968); and Holland et al., Biochemistry 17, 4900 (1978)). Suitable vectors and promoters for use in yeast expression are further described in R. Hitzeman et al., . EPO Pubin. No. 73,657. [0089] Cloned genes of the present invention may code for any species of origin, including mouse, rat, rabbit, cat, porcine, and human, but preferably code for Vitamin K dependent proteins of humah origin. DNA encoding Vitamin K dependent proteins that is* hybridizable with DNA encoding for proteins disclosed herein is also encompassed. -22- WO 2007/075976 PCT/US20061048954 Hybridization of such sequences may be carried out under conditions of reduced stringency or even stringent conditions (e.g., conditions represented by a wash stringency of 0.3M NaCl, 0.03M sodium citrate, 0.1% SDS at 60* C or even 70" C to DNA encoding the vitamin K dependent protein disclosed herein in a standard in situ hybridization assay. See J. Sambrook et al., Molecular Cloning, A Laboratory Manual (2d Ed. 1989)(Cold Spring Harbor Laboratory)). [0090] As noted above, preferred embodiments of the present invention provide methods of providing functional Vitamin K dependent proteins by methods which include ca'rboxylation of the N-terminal glu residues. The strategy may include co-expressing Vitamin K dependent protein along with VKOR, VKGC and/or PACS in a single host cell. In general, the method comprises culturing a host cell which expresses a vitamin K dependent protein and supporting proteins; and then harvesting the proteins from the culture. While some host cells may provide some Vitamin K dependent protein,. VKOR, VKGC and/or PACE at basal levels, in preferred embodiments, the vector DNA encoding PACE, VKGC and/or VKOR is included to enhance carboxylation. The culture can be carried out in any suitable fermentation vessel, with a growth media and under conditions -appropriate for the expression of the vitamin K dependent protein(s) by the particular host cell chosen. The Vitamin K dependent protein harvested from the culture is found to be carboxylated due to the expression of the supporting proteins in the host cell In preferred embodiments, vitamin K dependent protein can be collected directly from the culture media, or the host cells lysed and the vitamin K dependent protein collected therefrom. In preferred embodiments, vitamin K dependent protein can then be further purified in accordance with known techniques. 100911 As a general proposition, the purity of the recombinant protein produced according to the present invention will preferably be an appropriate purity known to the skilled art worker to lead to the optimal activity and stability of the protein. For example, when the recombinant protein is Factor IX , the Factor IX is preferably.of ultrahigh purity. Preferably, the recombinant protein has been subjected to multiple chromatographic purification steps, such as affinity chromatography, ion-exchange chromatography and preferably immunoaffinity chromatography to remove substances which cause fragmentation, activation and/or degradation of the recombinant protein during manufacture, storage and/or -23- WO 2007/075976 PCT/US2006/048954 use. Illustrative examples of such substances that are preferably removed by purification include thrombin and Factor IXa; other protein contaminants, such as modification enzymes like PACE/furin, VKOR, and VKGC; proteins, such as hamster proteins, which are released into the tissue culture media from the production cells during recombinant protein production; non-protein contaminants, such as lipids; and mixtures of protein and non-protein contaminants, such as lipoproteins. Purification procedures for vitamin K dependent proteins are known in the art. For example, see U.S. Patent No. 5,714,583, which is incorporated herein by reference. [00921 Factor IX DNA coding sequences, along with vectors and host cells for the expression thereof, are disclosed in European Patent App. 373012, European Patent App. 251874, PCT Patent Appl. 8505376, PCT Patent Appln. 8505125, European Patent Appln. 162782, and PCT Patent Appln. 8400560. Genes for other coagulation factors are also known .and available, for example, Factor II (Accession No. NM_000506), Factor VII (Accession No. NM_019616, and Factor X (Accession No. NM_000504). EXAMPLES Example 1. Primary transfection of CHO cells with Factor IX gene. [0093] A wild-type Factor IX gene was transfected into CHO cells by limit dilution into 96-well plates. The Factor IX gene was under the control of the CHEF-i promotor. Cells were allowed to grow in 5% serum for 14 days. The cell culture medium was -harvested and the total amount of Factor IX antigen in jig per mL was quantified by a Factor IX ELISA method. More than 150-clones were evaluated and the total amount of Factor IX produced per clone is reported in Figure 1. [0094] CHO cells transfected with the Factor IX gene produced Factor IX antigen which was detected by Factor IX ELISA. The amount varied significantly between clones. The range of total protein production after 14 days in culture was between 0 and greater than 1.6 pg/mL of culture medium. Although not determined in this experiment the Factor IX produced in primary transfectants was about 20% biologically active (data not shown) as determined in an APTT clotting assay using Factor IX-deficient plasma. Factor IX antigen can therefore be produced in CHO cells following transfection of the cells with wild type Factor IX. -24- WO 2007/075976 PCTUS2006/048954 Example 2. Supertransfection of Factor IX-producing CHO cells with VKGC and VKOR genes. 100951 In order to increase the percentage of active Factor IX produced in Factor IX-transfected CHO cells, the primary transfectants were pooled, expanded in tissue culture. and supertransfected with vectors containing cDNA for eniymes generally thought to be important for the efficient Vitamin K-dependent gania-carboxylation of Factor IX. Factor IX producing clones were pooled in a shake flask and supertransfected with cDNAs for both Vitamin K-dependent gamma-carboxylase (VKC) and Vitamin K-dependent epoxide reductase (VKOR). Individually supertransfected cells were grown by limit diltition in 96 well plates in 5% serum for 14 days. The total amount of Factor IX antigen produced per niL was measured by Factor IX ELISA. The amount of active Factor IX was measured by an APTT clotting assay using Factor IX-deficient plasma as substrate and- plasma-derived Factor IX as standard. Table 1. Supertransfection of Factor TX-producing CHO cell clones with VKGC and VKOR - FIX Specific Active % Active Clone Activity Activity FIX (pgtmL) (U/mL) (Umg) (pglmL) Fix 1 1.560 0.15 97 0.549 35 2 1.283 0.10 76 0.356 28 3 0.469 0.09 198 0.338 72 4 1.68 09 5 0.331 20 5 2i5 6.09 ~ 0.331 ~ 1-1 6 0.604 0.0 0.316 52 7 1.274 6.69 68. 0.316 26 8 0.811 0.09 105 0.309 38 9 0.827 0.08 100 0.302 36 10 0.954 0.07 7-, 0.25 2 11 0.177 0.03 86 0.120 68 12 0.340. 0.06 171 0.211.-62 13 0.121 0.02 165 0.073 60 14 0.272 0.04 143. .142 52 15 0.169 0.02 142 0.087 52 [0096) As seen in Table 1, the results of 15 individual clones were analyzed. The Factor IX antigen varied between 0.12 and 2.2 pg/mL. The percentage of active Factor IX -25- WO 2007/075976 PCT/US2006/048954 ranged between 15 and .72%. Consequently, the supertransfection cf Factor IX producing cells with VKGC and VKOR significantly increases the percentage of active Factor IX being produced by specific CHO cell clones. [0097] Note more antigen is produced as production is scaled up. For example, for 6-well plates, about 25-fold more antigen is produced when compared to 96-well plates. Consequently, in 6-well plates the levels of Factor IX antigen would be expected to range from 3-55 jgml. Example 3. Large-scale production of large quantities of biologically active recombinant Factor IX [00981 To demonstrate that Factor IX-producing CHO cells supertransfected with VKGC and VKOR can produce large quantities of biologically active Factor X, two independently isolated clones were grown in bioreactors and the quantity and quality of Factor IX product were evaluated after purifying the material. Bioreactors containing serum free medium were used to grow Clone 130 (12 L bioreactor) and Clone 44 (10 L bioreactor). Both of these clones expressed human Factor X, VKGC arid VKOR. The bioreactors were allowed to grow for 12 days without media change. The tissue culture fluid was separated from tlie cells and the Factor IX purified by a standard set of chromatography columns, resulting. in Factor IX protein with greater than 90% purity. Table 2. Large-Scale Production of biologically active recombinant Factor IX Clone Grown in Total Titer % Active Active Titer Bioreactor (mg/L) (mg/L) 130 44 61 27 44 28 35 10
    [
    009 9] As presented in Table 2, large quantities of Factor IX antigen were produced in both bioreactors. Clone 130 produced 44 mg of Factor IX per L of culture medium and Clone 44 produced 28 mg of Factor IX per L. Consistent with data presented earlier, the % active Factor IX was seen to be between 35 and 61 %. Consequently, Factor IX -26- WO 2007/075976 PCT/US2006/048954 producing CHO cells, when supertransfected with the posttranslational modification enzymes VKGC and VKOR, produce large quantities of Factor IX antigen that contains a significant amount of biologically active Factor IX. Example 4. Re-transfection with VKOR of clones producing Factor IX VKGC and VKOR [01001 In order to determine if it is possible to produce biologically active recombinant Factor IX in transfected CHO cells, the two clones, 130 and 44; which produced Factor IX after being supertransfected with VKGC and VKOR, were re-transfected with VKOR. Individual isolates of Clones 130 and 44 were cloned by limit dilution and re transfected with the cDNA for VKOR. The clones were grown up in 6-well plates and the cells were allowed to grow for 9 days until they were confluent. The total Factor IX antigen (gg per mL) was measured by Factor D ELISA, and the activity (U per mU) was determined by an APTT clotting assay using Factor TX-deficient plasma. -27- WO 2007/075976 PCT/US20061048954 Table 3. Re-transfection with VKOR of CHO clones producing Factor IX, VKGC and VKOR. Crone F-IX Titer F-ix Activty it Active (Ug/mL) (UfML) 130-1 2.7 065 199 00% 130 -2 2.6 OA 1N 74% 130-3 2.6 0.65 209 04% 130-4 1.3 0,23 172 69% 130-5 1.7 0,38 229 91% 130-6 1.1 0.16 145 58% 130-7 1.7 0.29 172 69% 13D-0 2.0 0A 229 92% 130-9 2.4 0.49 206 62% 130-10 1.9 0.42 222 89% 130-11 1.9 0.40 212 85% 13[-12 21 0.50 237 95% 130-13 2.2 0A 223 a9% 130-14 2.4 0.03 265 106% 130-15 2.2 OA 180 78% 130-16 1A . 0.31 214 86% 130-17 1.5 0,34 105 74% 130-tO 1.5 0.27 J 70 .70% 44-1 3.0 0.45 147 59% 44-2 0.9 0.22 235 04% 44-3 2.2 0,21 92 37% 44-4 1.3 0.26 210 84% 44-5 1.6 0.36 230 92% 44-6 1.1 0.22 194 78% 44.7 1.4 0.23 165 66% 44-8 0.9 0.15 163 55% 44-9 1.7 0.33 197 79% 44-10 1.6 0.25 156 62% 44-11 2.3 0.45 199 00% 44-12 1.4 0.34 240 96% 44-13 1.6 0.21 132 63% 44-14 1.9 0.26 136 55% 44-15 1.8 0.45 280 100% 44-16 2.1 042 194 77% [0101j The results in Table 3 show that subclones of both Clone 130 and Clone 44 produced significant quantities of Factor IX antigen, ranging -from 0.9 to 3.0 pg/mL Furthermore, as a consequence of the re-transfection with VKOR, both clones yielded at least one subclone that produced 100% of the Factor IX as biologically active protein, as well as several subelones with greater than 90% active Factor IX. These data suggest that adequate co-expression of VKGC and VKOR can facilitate production of totally highly active or eveft totally active Factor IX in CHO cells transfected with a wild type Factor IX cDNA. -28- WO 2007/075976 PCT/US2006/048954 Example 5. Large-scale Production of biologically active Factor IX in genetically engineered cells re-transfected with the post-translational modification enzyme VKOR. [0102] This experiment was designed to demonstrate that CHO cells producing recombinant Factor IX after transfection with VKGC and VKOR and re-transfected with VKOR can produce large quantities of Factor IX at production scale. Individual isolates of Clone 130 re-transfected with VKOR were grown up in 1.5 L shake flasks (to represent Commercial production) and the Factor IX antigen and biological activity were measured. Individual subclones of clone 130 described in EXAMPLE 4 above (CHO clone transfected with Factor IX, VKGC and VKOR and subsequently re-transfected with VKOR) were isolated by limit dilution in 6-well microtiter plates and then seeded into 1.5 L shaker flasks. Production of Factor IX in 1.5 L shaker flasks is known to reflect production conditions of 15 L and larger bioreactors (data not shown). The cells were allowed to grow in serum free media for 18 days, at which point samples were taken and evaluated for Factor IX antigen by a Factor IX ELISA and for biological activity by APTT clotting assay using Factor IX deficient plasma. Table 4. Production of large quantities of active Factor IX in CHO cells transfected with Factor LX, VKGC and VKOR, and re-transfected with VKOR Total Titer Active Titer Clone Flask (mg/L) % Active (mg/L) A 42.0 46.0 19.3 130 B 41.8 46.8 19.6 Average 41.9t 0.1 464 t 0.4 19.4* 0.1 A 45.1 49.5 22.3 130-6 B 42.8 52.2 22.4 Average 43.9 1 1.1 50.9 ± 1.3 22.3 20.1 A 41.5 48.7 . 20.2 130-16 B 37.1 513 19.0 Average 39.3 ±12.2 50.0 ± 1.3 19.6±:0.6 A 52.0 57.9 30.1 130-17 - 45.0 70.8 31.9 Average 48.5 * 3.5 64.3 ± 6.4 31.0 ± 0.9 A 53.8 52.6 28.3 130-19 50.6 59.0 29.8 Avenge 52.2:1:1.6 55.8 ± 3.2 29.1±:0.8 A 45.7 47.9 21.9 130-31 B 44.1 49.3 21.7 Average 44.9 ± 0.8 48.6 ± 0.7 21.8±0.1 -29- H:scAlnt~envoveIINRPottb1hDCCESCG48445_I.doc-i/08/201h3 [0103] The data for Clone 130 itself and for five subclones are presented in Table 4. Large quantities of Factor IX antigen were produced by all clones, ranging from 39.3 to 52.2 mg of Factor IX antigen per Liter of culture fluid. The percentage of active Factor IX was also quite high, ranging from 46.4% to 64.3%. The amount of biologically active Factor IX produced was also surprisingly high ranging from 19.4 to 31.0 mg/L. Consequently, in shaker flask systems, which reflect the production of Factor IX in commercial level bioreactors, large quantities of Factor IX antigen and active Factor IX can be produced in cells that have been transfected with Factor IX, VKGC, VKOR and subsequently re-transfected with VKOR. [01041 It will be understood by those of skill in the art that numerous and various modifications can be made without departing from the spirit of the present invention. Therefore, it should be clearly understood that the forms of the present invention are illustrative only and are not intended to limit the scope of the present invention. [0105] The reference in this specification to any prior publication (or information derived from it), or to any matter which is known, is not, and should not be taken as an acknowledgment or admission or any form of suggestion that that prior publication (or information derived from it) or known matter forms part of the common general knowledge in the field of endeavour to which this specification relates. [01061 Throughout this specification and the claims which follow, unless the context requires otherwise, the word "comprise", and variations such as "comprises" and "comprising", will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integers or steps. -30-
    权利要求:
    Claims (22)
    [1] 2. The method of claim 1, wherein the vitamin K dependent protein is selected from the group consisting of Factor II, Factor VII, Factor IX, Factor X, Protein C and Protein S.
    [2] 3. The method of claim 2, wherein the vitamin K dependent protein is Factor IX.
    [3] 4. The method of claim 2, wherein the vitamin K dependent protein is Factor VII.
    [4] 5. The method of claim 1, wherein the cell is further transfected with a gene encoding paired basic amino acid converting enzyme (PACE).
    [5] 6. The method of any one of claims 1 to 5, wherein at least about 75% of the glutamic acid residues within the gla-domain of the vitamin K dependent protein are gamma carboxylated.
    [6] 7. The method of any one of claims I to 6, wherein at least 25% of the vitamin K dependent protein is biologically active.
    [7] 8. The method of any one of claims 1 to 7, wherein at least 50% of the vitamin K dependent protein is biologically active. - 31 - H:vg~nwyvove nNRPoribTDMCCSCG53632 11 D0C-II1/2013
    [8] 9. The method of any one of claims 1 to 8, wherein at least 70% of the vitamin K dependent protein is biologically active.
    [9] 10. The method of any one of claims 1 to 9, wherein at least 80% of the vitamin K dependent protein is biologically active.
    [10] 11. The method of any one of claims I to 10, wherein the biologically active vitamin K dependent protein is produced in an amount of at least about 20 mg/L.
    [11] 12. The method of any one of claims 1 to 11, wherein transfection is sequential and wherein transfecting the mammalian cell further comprises: selecting for cells which express high levels of the vitamin K dependent protein product, VKOR or VKGC; cloning the selected cells; and amplifying the cloned cells.
    [12] 13. The method of claim 12, wherein the transfecting steps with the at least two genes are performed before the transfecting step with the gene encoding the vitamin K dependent protein.
    [13] 14. The method of claim 12, wherein the transfecting steps with the gene encoding the Factor IX protein are performed before the transfecting step with the at least two genes.
    [14] 15. The method of claim 1, wherein the mammalian cell is selected from the group consisting of CHO cells and HEK 293 cells.
    [15] 16. A recombinant mammalian cell comprising a gene encoding a vitamin K dependent protein linked to a CHEF-i promoter and at least two genes, wherein the at least two genes comprise a gene encoding vitamin K dependent epoxide reductase (VKOR) and a gene encoding vitamin K dependent -glutamyl carboxylase (VKGC); and wherein the cell -32- Hf:scgIntcniovenNRPortbl COSCG536382 I 1.DOC-A/2(l 13 produces biologically active vitamin K dependent protein in an amount of at least about 15 mg/L.
    [16] 17. A pharmaceutical composition comprising the recombinant vitamin K dependent protein produced by the method of any one of claims 1 to 16 and having at least 55% biological activity.
    [17] 18. A kit comprising the recombinant vitamin K dependent protein produced by the method of any one of claims I to 15.
    [18] 19. Use of the pharmaceutical composition of claim 17 in a method of treating hemophilia.
    [19] 20. A method of producing a recombinant biologically active vitamin K dependent protein product, comprising the steps of: (a) transfecting a mammalian cell with a gene encoding the vitamin K dependent protein linked to a CHEF-I promoter; (b) selecting for cells which express high levels of the vitamin K d ependent protein product; (c) transfecting the selected cells with at least two genes, wherein the at least two genes comprise a gene encoding vitamin K dependent reductase (VKOR), and a gene encoding vitamin K dependent-glutamyl carboxylase (VKGC); repeating step (b); cloning the selected cells; amplifying the cloned cells; and harvesting the product from the cloned cells, wherein the cell produces recombinant biologically active vitamin K dependent protein in an amount of at least 15 mg/L.
    [20] 21. The method of claim 20, wherein the method further comprises repeating steps (a) and/or (c) followed by (b), before the step of cloning the selected cells. - 33 - H:scgintnrmvenNRPortblDCCSCG5362 1_1 DDC-IA18/2013
    [21] 22. A method of treating hemophilia comprising administering to a subject in need thereof a therapeutically effective amount of the composition of claim 17.
    [22] 23. A method according to any one of claims 1 to 15 or 20 to 22, or a recombinant mammalian cell according to claim 16, or a composition according to claim 17, or a kit according to claim 18, or use according to claim 19, substantially as hereinbefore described with reference to any one of the Examples and/or Figure. - 34 -
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    同族专利:
    公开号 | 公开日
    AU2013211534B2|2016-03-03|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    法律状态:
    2013-10-03| PC1| Assignment before grant (sect. 113)|Owner name: UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL; CNJ H Free format text: FORMER APPLICANT(S): CANGENE CORPORATION; UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL |
    2016-06-30| FGA| Letters patent sealed or granted (standard patent)|
    2017-02-23| PC| Assignment registered|Owner name: THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL Free format text: FORMER OWNER(S): CNJ HOLDINGS, INC.; UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL Owner name: APTEVO BIOTHERAPEUTICS LLC Free format text: FORMER OWNER(S): CNJ HOLDINGS, INC.; UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL |
    优先权:
    申请号 | 申请日 | 专利标题
    US60/752,642||2005-12-21||
    AU2006331501A|AU2006331501B2|2005-12-21|2006-12-21|Method of producing biologically active vitamin K dependent proteins by recombinant methods|
    AU2013211534A|AU2013211534B2|2005-12-21|2013-08-02|Method of producing biologically active vitamin K dependent proteins by recombinant methods|AU2013211534A| AU2013211534B2|2005-12-21|2013-08-02|Method of producing biologically active vitamin K dependent proteins by recombinant methods|
    AU2016200807A| AU2016200807A1|2005-12-21|2016-02-09|Method of producing biologically active vitamin K dependent proteins by recombinant methods|
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