Sulfonamide compounds for the treatment of neurodegenerative disorders

专利摘要:

公开号:AU2005227749A1
申请号:U2005227749
申请日:2005-03-21
公开日:2005-10-13
发明作者:Michael A. Brodney；Karen J. Coffman
申请人:Pfizer Products Inc；
IPC主号:C07D257-04

专利说明:
WO 2005/095334 PCT/IB2005/000796 -1 SULFONAMIDE COMPOUNDS FOR THE TREATMENT OF NEURODEGENERATIVE DISORDERS Cross Reference to Related Application The present application claims benefit of U.S.S.N. 60/558,660 filed on April 1, 2004, 5 which is incorporated by reference herein in its entirety. Field of the Invention The present invention relates to the treatment of neurodegenerative and/or neurological disorders, such as Alzheimer's disease, in mammals, including humans. This invention also relates to inhibiting, in mammals, including humans, the production of Ap 10 peptides that can contribute to the formation of neurological deposits of amyloid protein. More particularly, this invention relates to sulfonamide compounds, pharmaceutical compositions comprising such compounds and methods of using such compounds, i.e., for the treatment of neurodegenerative and/or neurological disorders, such as Alzheimer's disease, related to Ap-peptide production. 15 Background of the Invention Dementia results from a wide variety of distinctive pathological processes. The most common pathological processes causing dementia are Alzheimer's disease (AD), cerebral amyloid angiopathy (CAA) and prion-mediated diseases. AD affects nearly half of all people past the age of 85, the most rapidly growing portion of the United States population. As such, 20 the number of AD patients in the United States is expected to increase from about 4 million to about 14 million by the middle of the next century. Treatment of AD typically is the support provided by a family member in attendance. Stimulated memory exercises on a regular basis have been shown to slow, but not stop, memory loss. A few drugs, for example AriceptTM, provide treatment of AD. 25 A hallmark of AD is the accumulation in the brain of extracellular insoluble deposits called amyloid plaques and abnormal lesions within neuronal cells called neurofibrillary tangles. Increased plaque formation is associated with an increased risk of AD. Indeed, the presence of amyloid plaques, together with neurofibrillary tangles, is the basis for definitive pathological diagnosis of AD. 30 The major components of amyloid plaques are the amyloid Ap-peptides, also called Ap-peptides, that consist of several proteins including those having 38, 40, 42 or 43 amino acids, designated as the Ap 1 -sa, Ap3 1 r A0 1 .4 2 and Ap 1 .4 3 peptides, respectively. The Ap peptides are thought to cause nerve cell destruction, in part, because they are toxic to neurons in vitro and in vivo. 35 The As peptides are derived from larger amyloid precursor proteins (APP proteins), that consist of four proteins containing 695, 714, 751 or 771 amino acids, designated as the
APP
695 , APP 7 1 4 , APP 751 and APP 771 , respectively. Proteases are believed to produce the AP WO 2005/095334 PCT/IB2005/000796 -2 peptides by cleaving specific amino acid sequences within the various APP proteins. The proteases are named "secretases" because the Ap-peptides they produce are secreted by cells into the extracellular environment. These secretases are each named according to the cleavage(s) they make to produce the Ap-peptides. The secretase that forms the amino 5 terminal end of the Ap-peptides is called the beta-secretase. The secretase that forms the carboxyl terminal end of the AP-peptides is called the gamma-secretase. This invention relates to novel compounds that inhibit AP-peptide production, to pharmaceutical compositions comprising such compounds, and to methods of using such compounds to treat neurodegenerative and/or neurological disorders. 10 Summary of the Invention The present invention relates to compounds of the Formula I (Ri)m R 3 0 /S INH 2 0 0 (R2)n wherein R' and R 2 are each independently selected from -H, -C-Ca alkyl, -C2C8 alkenyl, -C C8 alkoxy, -C2-C8 alkenoxy, -C-C8 hydroxyalkyl, -C2-Ca hydroxyalkenyl, -halo, -CN, -NO 2 , 15 (CH 2 )qNC(=0)R 6 , -(CH 2 )qC(=0)OR 5 , -(CH 2 )qC(=0)NRR, -(CH 2 )qNR R, -C3-C8 cycloalkyl and -C5-C8 cycloalkeny, wherein R 1 and R 2 are each optionally independently substituted with from one to three substituents independently selected from -C-Co alkoxy, -1-Cr alkyl -halo and -OH;
R
3 is a group of the Formula 11 or the Formula IlIl, each as depicted below Y, z B 20 WO 2005/095334 PCT/IB2005/000796 -3 Y I"-z x-D B 111 wherein B is absent or is selected from -C-C8 alkylene and -C2-C4 alkenylene; D is selected from -C-Ca alkyl, -C 3
-C
10 cycloalkyl, -(3-10 membered) heterocycloalkyl and -(5-7 membered) heteroaryl; 5 X, Y and Z are each independently selected from -H, -0 1
-C
8 alkyl, -C2-C8 alkenyl, -C C8 alkoxy, -C 2
-C
8 alkenoxy, -C-CB hydroxyalky, -C2-Ca hydroxyakenyl, -halo, -OH, -CN, NO 2 , -(CH 2 )qNR R, -(CH 2 )q-NHC(=O)R 9 , -(CH 2 )qC(=O)R 9 , -(CH 2 )qC(=O)NRR 5 , -(CH2)q C(=O)OR, -(CH 2
)-SO
2
R
9 , -S(C-Ca alkyl), -C3-Ca cycloalkyl, -(CH2)q-(( 3 -10 membered) heterocycloalkyl), -(CH2)q-(Cs-CI4 aryl), -(CH 2 )q-((4-10 membered) heteroaryl) and -(CH2), 10 (C6-C14 aryloxy); wherein said X, Y and Z are each optionally independently substituted with from one to three substituents independently selected from -Cr-C8 alkyl, -C2-Ca alkenyl, -C-C8 alkoxy, -C2-Ca alkenoxy, C-Ca hydroxyalkyl, -C2-Ca hydroxyalkenyl, halo, -OH and -CN; and wherein said alkoxy substituent of X, Y and Z is optionally independently 15 substituted with from one to three substituents independently selected from halo, preferably F; R , R 6 R, R and R are each independently selected from -H, -C 1 rC 1 2 alkyl, -C2-C12 alkeny, -C3-C10 cycloalkyl, -C5-C10 cycloalkenyl, -Cs-C13 bicycloalkyl, -C7-CI3 bicycloalkenyl, (3-10 membered) heterocycloalkyl, -CS-C14 aryl and -(5-8 membered) heteroaryl; 20 wherein NR 6
R
7 or NR 7
R
8 may in each instance independently optionally form a -(3-8 membered) heterocycloalkyl; m is 0,1, 2 or 3; n is 0,1, 2 or 3; q is 0, 1 or2; 25 or the pharmaceutically acceptable salts of such compounds. Compounds of the Formula I may have optical centers and therefore may occur in different enantiomeric and diastereomeric configurations. The present invention includes all enantiomers, diastereomers, and other stereoisomers of such compounds of the Formula l, as well as racemic compounds and racemic mixtures and other mixtures of stereoisomers 30 thereof. Pharmaceutically acceptable salts of the compounds of Formula I include the acid addition and base salts thereof.
WO 2005/095334 PCT/IB2005/000796 -4 Suitable acid addition salts are formed from acids which form non-toxic salts. Examples include, but are not limited to, the acetate, adipate, aspartate, benzoate, besylate, bicarbonate/carbonate, bisulphate/suiphate, borate, camsylate, citrate, cyclamate, edisylate, esylate, formate, fumarate, gluceptate, gluconate, glucuronate, hexafluorophosphate, 5 hibenzate, hydrochloride/chloride, hydrobromide/bromide, hydroiodideliodide, isethionate, lactate, malate, maleate, malonate, mandelates mesylate, methylsulphate, naphthylate, 2 napsylate, nicotinate, nitrate, orotate, oxalate, palmitate, pamoate, phosphate/hydrogen phosphate/dihydrogen phosphate, pyroglutamate, salicylate, saccharate, stearate, succinate, sulfonate, stannate, tartrate, tosylate, trifluoroacetate and xinofoate salts. 10 Suitable base salts are formed from bases which form non-toxic salts. Examples include, but are not limited to, the aluminium, arginine, benzathine, calcium, choline, diethylamine, diolamine, glycine, lysine, magnesium, meglumine, olamine, potassium, sodium, tromethamine and zinc salts. Hemisalts of acids and bases may also be formed, for example, hemisulphate and 15 hemicalcium salts. For a review on suitable salts, see Handbook of Pharmaceutical Salts: Properties, Selection, and Use by Stahl and Wermuth (Wiley-VCH, 2002). Pharmaceutically acceptable salts of compounds of Formula I may be prepared by one or more of three methods: 20 (i) by reacting the compound of Formula I with the desired acid or base; (ii) by removing an acid- or base-labile protecting group from a suitable precursor of the compound of Formula I or by ring-opening a suitable cyclic precursor, for example, a lactone or lactam, using the desired acid or base; or (iii) by converting one salt of the compound of Formula I to another by reaction 25 with an appropriate acid or base or by means of a suitable ion exchange column. All three reactions are typically carried out in solution. The resulting salt may precipitate out and be collected by filtration or may be recovered by evaporation of the solvent. The degree of ionization in the resulting salt may vary from completely ionised to almost non-ionised. 30 The compounds of the invention may exist in a continuum of solid states ranging from fully amorphous to fully crystalline. The term 'amorphous' refers to a state in which the material lacks long range order at the molecular level and, depending upon temperature, may exhibit the physical properties of a solid or a liquid. Typically such materials do not give distinctive X-ray diffraction patterns and, while exhibiting the properties of a solid, are more 35 formally described as a liquid. Upon heating, a change from solid to liquid properties occurs which is characterised by a change of state, typically second order ('glass transition'). The term 'crystalline' refers to a solid phase in which the material has a regular ordered internal WO 2005/095334 PCT/IB2005/000796 -5 structure at the molecular level and gives a distinctive X-ray diffraction pattern with defined peaks. Such materials when heated sufficiently will also exhibit the properties of a liquid, but the change from solid to liquid is characterised by a phase change, typically first order ('melting point'). 5 The compounds of the invention may also exist in unsolvated and solvated forms. The term 'solvate' is used herein to describe a molecular complex comprising the compound of the invention and one or more pharmaceutically acceptable solvent molecules, for example, ethanol. The term 'hydrate' is employed when said solvent is water. - A currently accepted classification system for organic hydrates is one that defines 10 isolated site, channel, or metal-ion coordinated hydrates - see Polymorphism in Pharmaceutical Solids by K. R. Morris (Ed. H. G. Brittain, Marcel Dekker, 1995). Isolated site hydrates are ones in which the water molecules are isolated from direct contact with each other by intervening organic molecules. In channel hydrates, the water molecules lie in lattice channels where they are next to other water molecules. In metal-ion coordinated hydrates, 15 the water molecules are bonded to the metal ion. When the solvent or water is tightly bound, the complex will have a well-defined stoichiometry independent of humidity. When, however, the solvent or water is weakly bound, as in channel solvates and hygroscopic compounds, the water/solvent content will be dependent on humidity and drying conditions. In such cases, non-stoichiometry will be the 20 norm. Also included within the scope of the invention are multi-component complexes (other than salts and solvates) wherein the drug and at least one other component are present in stoichiometric or non-stoichiometric amounts. Complexes of this type include, but are not limited to, clathrates (drug-host inclusion complexes) and co-crystals. The latter are typically 25 defined as crystalline complexes of neutral molecular constituents which are bound together through non-covalent interactions, but could also be a complex of a neutral molecule with a salt. Co-crystals may be prepared by melt crystallisation, by recrystallisation from solvents, or by physically grinding the components together - see Chem Commun, 17, 1889-1896, by 0. Almarsson and M. J. Zaworotko (2004). For a general review of multi-component complexes, 30 see J Pharm Sci, 64 (8), 1269-1288, by Haleblian (August 1975). The compounds of the invention may also exist in a mesomorphic state (mesophase or liquid crystal) when subjected to suitable conditions. The mesomorphic state is intermediate between the true crystalline state and the true liquid state (either melt or solution). Mesomorphism arising as the result of a change in temperature is described as 35 'thermotropic' and that resulting from the addition of a second component, such as water or another solvent, is described as 'lyotropic'. Compounds that have the potential to form lyotropic mesophases are described as 'amphiphilic' and consist of molecules which possess WO 2005/095334 PCT/IB2005/000796 -6 an ionic (such as -COO~Na*, -COO'K*, or -SOs~Na*) or non-ionic (such as -N-N'(CH 3
)
3 ) polar head group. For more information, see Crystals and the Polarizing Microscope by N. H. Hartshorne and A. Stuart, 4t Edition (Edward Arnold, 1970). Hereinafter all references to compounds of Formula I include references to salts, 5 solvates, multi-component complexes and liquid crystals thereof and to solvates, multi component complexes and liquid crystals of salts thereof. The compounds of the invention include compounds of Formula I as hereinbefore defined, including all polymorphs and crystal habits thereof, prodrugs and isomers thereof (including optical, geometric and tautomeric isomers) as hereinafter defined and isotopically 10 labeled compounds of Formula 1. Unless otherwise indicated, as used herein, the term "B is absent" means a direct bond between the nitrogen and the other groups (e.g., -N-D). Unless otherwise indicated, as used herein, the terms "halo" and "halogen" include F, Cl, Br and I. 15 Unless otherwise indicated, as used herein, the term "alkyl" includes saturated monovalent hydrocarbon radicals having straight or branched moieties. Examples of alkyl groups include, but are not limited to, methyl, ethyl, n-propyl, isopropyl and t-butyl. Unless otherwise indicated, as used herein, the term "alkenyl" includes alkyl moieties having at least one carbon-carbon double bond wherein alkyl is as defined above. Examples 20 of alkenyl include, but are not limited to, ethenyl and propenyl. Unless otherwise indicated, as used herein, the term "alkylene" includes saturated, divalent hydrocarbon radicals i.e., generally present as a bridging group between two other groups, having straight or branched moieties. Examples of alkylene groups include
-CH
2 - (methylene); -CH 2
CH
2 - (ethylene); -CH 2
CH
2
CH
2 - (propylene), -CH(CH 3
)CH
2 25 (isopropylene) etc. Unless otherwise indicated, as used herein, the term "alkenylene" includes divalent hydrocarbon radicals with at least one carbon-carbon double bond and may include straight or branched alkenylene groups, such as ethyenylene. Alkenylene groups are generally present as a bridging group between two other groups. 30 Unless otherwise indicated, as used herein, the term "cycloalkyl" includes non aromatic saturated cyclic alkyl moieties wherein alkyl is as defined above. Examples of cycloalkyl include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl and cycloheptyl. "Bicycloalkyl" and "tricycloalkyl" groups are non-aromatic saturated carbocyclic groups consisting of two or three rings respectively, wherein said rings share at least one 35 carbon atom. Unless otherwise indicated, for purposes of the present invention, bicycloalkyl groups include spiro groups and fused ring groups. Examples of bicycloalkyl groups include, but are not limited to, bicyclo-[3.1.0]-hexyl, bicyclo-2.2.1]-hept-1-yl, norbornyl, WO 2005/095334 PCT/IB2005/000796 -7 spiro[4.5]decyl, spiro[4.4]nonyl, spiro[4.3]octyl, and spiro[4.2]heptyl. An example of a tricycloalkyl group is adamantanyl. Other cycloalkyl, bicycloalkyl and tricycloalkyl groups are known in the art, and such groups are encompassed by the definitions "cycloalkyl," "bicycloalkyl" and "tricycloalkyl" herein. "Cycloalkenyl," "bicycloalkenyl" and "tricycloalkenyl" 5 refer to non-aromatic carbocyclic cycloalkyl, bicycloalkyl and tricycloalkyl moieties as defined above, except that cycloalkenyl, bicycloalkenyl and tricycloalkenyl comprise one or more carbon-carbon double bonds connecting carbon ring members (an "endocyclic" double bond) and/or one or more carbon-carbon double bonds connecting a carbon ring member and an adjacent non-ring carbon (an "exocyclic" double bond). Examples of cycloalkenyl groups 10 include, but are not limited to, cyclopentenyl, cyclobutenyl and cyclohexenyl, and a non limiting example of a bicycloalkenyl group is norbornenyl. Cycloalkyl, cycloalkenyl, bicycloalkyl and bicycloalkenyl groups also include groups that are substituted with one or more oxo moieties. Examples of such groups with oxo moieties are oxocyclopentyl, oxocyclobutyl, oxocyclopentenyl and norcamphoryl. Other cycloalkenyl, bicycloalkenyl, and tricycloalkenyl 15 groups are known in the art, and such groups are included within the definitions "cycloalkenyl," "bicycloalkenyl" and "tricycloalkenyl" herein. Unless otherwise indicated, as used herein, the term "aryl" includes an organic radical derived from an aromatic hydrocarbon by removal of one hydrogen, and includes such groups as phenyl, naphthyl, indenyl, indanyl, tetralinyl and fluorenyl. "Aryl" encompasses fused ring 20 groups wherein at least one ring is aromatic. Unless otherwise indicated, as used herein, the terms "heterocyclic," "heterocycloalkyl," and like terms refer to non-aromatic cyclic groups containing one or more heteroatoms, prefereably from one to four heteroatoms, each selected from 0, S and N. "Heterobicycloalkyl" groups are non-aromatic two-ringed cyclic groups, wherein said rings share 25 one or two atoms, and wherein at least one of the rings contains a heteroatom (0, S, or N). Heterobicycloalkyl groups for purposes of the present invention, and unless otherwise indicated, include spiro groups and fused ring groups. The heterocyclic (i.e. heterocycloalkyl, heterobicycloalkyl) groups of the compounds of the subject invention can include 0, S(O)zero-2, and/or N as heteroatoms, wherein wherein the subscript "zero-2" of S(O)zero-2 represents a 30 group of integers consisting of zero, 1 and 2. Thus, S(O)zero-2 represents the group consisting of S, S(=O), and S(0)2. In one embodiment, each ring in the heterobicycloalkyl contains up to four heteroatoms (i.e., from zero to four heteroatoms, provided that at least one ring contains at least one heteroatom). The heterocyclic groups, including the heterobicyclic groups, of this invention can also include ring systems substituted with one or more oxo moieties. The heterocyclic 35 groups, including the heterobicyclic groups, may comprise double bonds, e.g., heterocycloalkenyl, heterobicycloalkenyl. Examples of non-aromatic heterocyclic groups are aziridinyl, azetidinyl, pyrrolidinyl, piperidinyl, azepinyl, piperazinyl, 1,2,3,6-tetrahydropyridinyl, WO 2005/095334 PCT/IB2005/000796 -8 oxiranyl, oxetanyl, tetrahydrofuranyl, tetrahydrothienyl, tetrahydropyranyl, tetrahydrothiopyranyl, morpholino, thiomorpholino, thioxanyl, pyrrolinyl, indolinyl, 2H-pyranyl, 4H-pyranyl, dioxanyl, 1,3-dioxolanyl, pyrazolinyl, dihydropyranyl, dihydrothienyl, dihydrofuranyl, pyrazolidinyl, imidazolinyl, imidazolidinyl, 3-azabicyclo[3.1.0]hexanyl, 3 5 azabicyclo[4.1.0]heptanyl, quinolizinyl, quinuclidinyl, 1,4-dioxaspiro[4.5]decyl, 1,4 dioxaspiro[4.4]nonyl, 1,4-dioxaspiro[4.3]octyl and 1,4-dioxaspiro[4.2]heptyl. Unless otherwise indicated, as used herein, "heteroaryl" refers to aromatic groups containing one or more heteroatoms (0, S, or N), preferably from one to four heteroatoms. A multicyclic group containing one or more heteroatoms wherein at least one ring of the group is 10 aromatic is a "heteroaryl" group. The heteroaryl groups of this invention can also include ring systems substituted with one or more oxo moieties. Examples of heteroaryl groups are pyridinyl, pyridazinyl, imidazolyl, pyrimidinyl, pyrazolyl, triazolyl, pyrazinyl, quinolyl, isoquinolyl, 1,2,3,4-tetrahydroguinoly, tetrazolyl, furyl, thienyl, isoxazolyl, thiazolyl, oxazolyl, isothiazolyl, pyrrolyl, indolyl, benzimidazolyl, benzofuranyl, cinnolinyl, indazolyl, indolizinyl, phthalazinyl, 15 triazinyl, 1,2,4-trizainyl, 1,3,5-triazinyl, isoindolyl, 1-oxoisoindolyl, purinyl, oxadiazolyl, thiadiazolyl, furazanyl, benzofurazanyl, benzothiophenyl, benzotriazolyl, benzothiazolyl,, benzoxazolyl, quinazolinyl, quinoxalinyl, naphthyridinyl, dihydroquinolyl, tetrahydroquinolyl, dihydroisoquinolyl, tetrahydroisoquinolyl, benzofuryl, furopyridinyl, pyrolopyrimidinyl and azaindolyl. 20 As appreciated by the artisan, the use of Formula I is a convenience and the invention is understood to envision and embrace each and every species hereunder as though individually identified and set forth herein. Thus the present invention contemplates each species separately and any and all combinations and permutations of species falling within Formula 1. 25 The foregoing groups, as derived from the compounds listed above, may be C-attached or N-attached where such is possible. For instance, a group derived from pyrrole may be pyrrol-1-yl (N-attached) or pyrrol-3-yl (C-attached). The terms referring to the groups also encompass all possible tautomers. The subject invention also includes all prodrugs of compounds of the Formula I. A 30 prodrug is a compound that may not possess the desired pharmacological activity per se, but can be administered, for example parenterally or orally, to a mammal, thereafter being metabolized in the mammal's body to form a compound that does have the desired pharmacological activity. For example, a prodrug of a compound of the Formula I is metabolized, after administration to a mammal, to a compound of the Formula 1. Further, it will 35 be appreciated by those skilled in the art that certain protected derivatives of compounds of the Formula 1, that may be made prior to a final deprotection stage, may, in certain instances, be administered to a mammal and thereafter metabolized in the mammal's body to form compounds WO 2005/095334 PCT/IB2005/000796 -9 of the invention that are pharmacologically active. Such derivatives are therefore also "prodrugs" of compounds of the Formula I and are part of the present invention. In another aspect, the present invention relates to compounds of the Formula I wherein R' is halo, C-C 4 alkyl, C-C 4 alkoxy, and wherein said alkyl and alkoxy are optionally 5 substituted with 1 to 3 halo atoms. In one aspect, the present invention relates to compounds of the Formula I wherein
R
1 is halo, preferably -Cl, and m is 1. In another aspect, the present invention relates to compounds of the Formula I wherein R 2 is -H; 10 In another aspect, the present invention relates to compounds of the Formula I wherein R 3 is a group of the Formula 1i, as depicted below Y z X-D B 111 wherein D is -C-C 8 alkyl; and X, Y, Z and B are as defined above, and wherein said X, Y and Z are each optionally independently substituted as defined above. 15 In another aspect, R 3 is a group of the Formula 11, as depicted below z X B wherein X, Y, Z and B are as defined above, and wherein said X, Y and Z are each optionally independently substituted as defined above. In another aspect, R 3 is a group of the Formula 11, as depicted below z x B 20 wherein B is C-C 4 alkylene; X, Y and Z are each independently selected from -H, -0 1
-C
8 alkyl, -C2-Ca alkenyl, -C Ca alkoxy, -C 2 -Ca alkenoxy, -halo, -OH, -CN, -NO 2 , -(CH 2 )q-NR 7
R
8 , -(CH 2 )q-C(=O)NRR, (CH 2 )q-C(=O)R 9 , -(CH 2 )q-C(=O)OR, -S(C-Ca alkyl), -(CH 2 )q-SO 2
R
9 , -(CH2)q-(( 3 -1 0 WO 2005/095334 PCT/IB2005/000796 -10 membered) heterocycloalkyl), -(CH2)q-(C-C14 aryl), -(CH2)q-(( 4 -1 0 membered) heteroaryl) and -(CH2)a-(C-Cl4 aryloxy); wherein said X, Y and Z are each optionally independently substituted with from one to three substituents independently selected from halo, -OH and -CN; and 5 q is 0, 1 or 2. In another aspect, R 3 is a group of the Formula 11, as depicted below z X B wherein B is methylene; q is 1; and X, Y and Z are as defined immediately above, wherein said X, Y and Z are each optionally independently substituted as defined immediately above. 10 In another aspect, R 3 is a group of the Formula 11, as depicted below z B it wherein B is C-C 8 alkylene; X, Y and Z are each independently selected from -H, -Cr1C8 alkyl, -C2-Ca alkenyl, -C C8 alkoxy, -C2-Ca alkenoxy, -halo, -OH, -CN, -NO 2 , -(CH 2 )q-NR 7
R
8 , -(CH 2 )q-C(=0O)NR 7
R
8 , 15 (CH 2 )q-C(=0)R 9 , -(CH 2 )q-C(=O)OR 9 , -S(C-C 8 alkyl), -(CH 2 )q-SO 2
R
9 , -(CH2)q-(( 3- 1 0 membered) heterocycloalkyl), -(CH2)q-(Co-C14 aryl), -(CH 2 )q-((4-10 membered) heteroaryl) and -(CH2)q-(C6-C14 aryloxy); wherein said alkyl, alkenyl, alkoxy, alkenoxy, -S(C-C 8 alkyl), aryl and aryloxy of X, Y and Z are each optionally independently substituted with from one to three substituents 20 independently selected from halo, -OH and -CN; and q is 0,1 or 2. In another aspect, R 3 is a group of the Formula 11, as depicted below 4z B wherein B is C-Ca alkylene; q is 0, 1 or 2; and the aryl of said -(CH2)q-(C-C14 aryl) of X, Y 25 and Z is phenyl, the R of said -(CH 2 )q-C(=O)R 9 and of said -(CH 2 )q-SO 2
R
9 , both of X, Y and WO 2005/095334 PCT/IB2005/000796 -11 Z, is phenyl, and the aryloxy of said -(CH2)q-(C6-C4 aryloxy) of X, Y and Z is phenoxy, wherein said X, Y and Z are each optionally independently substituted as defined immediately above. In another aspect, R 3 is a group of the Formula 11, as depicted below Y z x B 5 wherein B is C-C 8 alkylene; q is 0, 1 or 2; and the heterocycloalkyl of said -(CH2)q-( 3 -10 membered) heterocycloalkyl of X, Y and Z is selected from pyrrolidinyl and morpholinyl, wherein said X, Y and Z are each optionally independently substituted as defined above. In another aspect, R 3 is a group of the Formula 11, as depicted below z B 101 10 1^'^ , wherein B is -C 1
-C
8 alkylene; q is 0, 1 or 2; and the heteroaryl of said -(CH2)q-( 4 -10 membered) heteroaryl of X, Y and Z is selected from imidazolyl, thiadiazolyl, oxazolyl, pyrazolyl, isoxazolyl and tetrazolyl, wherein said X, Y and Z are each optionally independently substituted as defined above. 15 In another aspect, R 3 is aryl or benzyl. Specific embodiments of the present invention include the following compounds of Formula I, all pharmaceutically acceptable salts thereof, complexes thereof, and derivatives thereof that convert into a pharmaceutically active compound upon administration: 4-{[(1-Carbamoyl-2-pheny-ethyl)-(4-chloro-benzenesulfonyl)-amino]-methyl}-benzoic 20 acid methyl ester; 4-{[(CarbamoyI-phenyl-methyl)-(4-chloro-benzenesulfonyl)-amino]-methyl}-benzoic acid methyl ester; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-dimethylaminomethyl-benzyl)-amino]-2-phenyl acetamide; 25 (R)-2-[(4-Chloro-benzenesulfonyl)-(4-morpholin-4-ylmethyl-benzyl)-amino]-2-phenyl acetamide; (R)-2-[Benzyl-(4-chloro-benzenesulfonyl)-amino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-cyano-benzyl)-amino]-2-phenyl-acetamide; (R)-2-[(4-tert-Butyl-benzyl)-(4-chloro-benzenesulfonyl)-amino]-2-phenyl-acetamide; WO 2005/095334 PCT/1B2005/000796 -12 (R)-2-[(4-Chloro-benzenesufony)-(3-yalo-bel)-amfilo]-2-pheny-acetafl1ide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-trifluoromethoxy-belzyl)-amino]-2-phenyl acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-[1 ,2,3lth iadiazol-4-yI-benzyl )-amino]-2-phenyl 5 acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-oxazo-2-yI-belzyl)-am ino]-2-phenyl-acetamide; (R)-2-{(4-Chloro-benzenesulfonyl)-[4-(3-methyl-LI ,2,4]oxadiazol-5-yl)-benzyl]-amino} 2-phenyl-acetamide; (R)-2-{(4-Chloro-benzenesulfonyl)-[4-(2-methyl-2H-tetrazol-5-yl)-benzyI-amilo}-2 10 phenyl-acetamide; (R)-2-{(4-Chloro-benzenesulfonyl)-[4-(1 -hydroxy-1 -methyl-ethyl)-benzylj-am ino}-2 phenyl-acetamide; (R)-2-(2,2-Oimethyl-propylamino)-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfoflyl)-(2,2-dimethyl-propyI)-aminol-2-pheflyl-acetamide; 15 (R)-2-[(4-Chloro-benzenesulfonyl)-(2-methyl-benzyl)-amilo]-2-phefl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(3-methyl-benzyl)-am ino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(3-chloro-benzyl )-aminol-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(2-cyano-benzyl )-amino]-2-phenyl-acetamide; (R)-2-[(4-Ch orobenzenesulfonyI)-(3-fluoro-benzyl)-aminoI-2-phelyl-acetam ide; 20 (R)-2-[(4-Chloro-benzenesulfonyl)-(4-fluoro-belzy)-alilo]-2-phel-acetaml ide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-trifluoromethyl-benzy )-amino]-2-phenyl acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(2,4-difluoro-benzyl)-amino]-2-phel-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(3,4-d ifluoro-benzyl)-am ino]-2-phenyl-acetam ide; 25 (R)-2-[(4-Chloro-benzenesulfoflyl)-(3-trifluoromethyl-bel)-amilo]-2-phelyl acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(2-trifluoromethyl-benzyl)-aminoI-2-phel acetamide; (R)-2-[(4-Chloro-benzenesulfonyI)-(2-chloro-benzyl)-amiloI-2-pheflyl-acetamide; 30 (R)-2-[(4-Chloro-benzenesulfonyl)-(2,6-dichloro-benfly)-amiflo]-2-phel-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(2-fluoro-benzyl)-aminol-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(2,6-difluoro-benzy)-amilo]-2-phefl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(2-chloro-5-trifluoromethyl-belzyl)-amilol-2 phenyl-acetamide; 35 (R)-2-[(4-Chloro-benzenesulfonyl)-(3-difluoromethoxy-bel)-amilol-2-phelyl acetam ide; WO 2005/095334 PCT/1B2005/000796 -13 (R)-2.-[(4-Chloro-benzenesulfonyl)-(3-fluoro-4-trifluoromethyl-benzyl )-amino]-2-phenyl acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(5-fuoro-2-trifluoromethyl-benzy )-am ino]-2-phenyl acetamide; 5 (R)-2-[(4-Chloro-benzenesulfonyl)-(2-trifluoromethoxy-benzy )-am ino]-2-phenyl acetamide; (R)-4-{[(Carbamoyl-phenyl-methyl)-(4-chloro-benzenesulfonyl)-am ino]-methyl}-3 methoxy-benzoic acid methyl ester; (R)-2-[Biphenyl-2-ylmethyl-(4-chloro-benzenesulfoflyl)-ailo]-2-phenyl-acetam ide; 10 (R)-2-[(2-Benzenesulfonylmethyl-benzyl)-(4-chloro-benzenesulfonyl)-amino]-2 phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(2,3-difluoro-benzy)-am ino]-2-phenyl-acetam ide; (R)-2-[(4-Chioro-benzenesulfonyl )-(3,5-difluoro-benzyl)-am ino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(2,3,6-trifluoro-benzyl)-amino]-2-phenyl-acetamide; 15 (R)-3-{[(Carbamoyi-phenyl-methyi)-(4-chloro-benzenesulfonyl )-am ino]-methyl} benzoic acid methyl ester; (R)-2-[(4-Chloro-benzenesulfonyl)-(3,4-dichloro-benzyl)-amino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(2-fiuoro-3-methyl-benzyl)-am ino]-2-phenyl acetamide; 20 (R)-2-[(4-Chloro-benzenesulfonyl)-(2,4,5-trifluoro-benzyl)-am ino]-2-pheriyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(2,4,6-trifluoro-benzy)-am ino]-2-phenyl-acetamide; (R)-2-[(4-Ghloro-benzenesulfonyl)-(2-chloro-4-fluoro-benzyl)-amino]-2-phenyl acetamide; (R)-2-{(4-Chloro-benzenesulfonyl)-[3-(4-fluoro-phenoxy)-benzyl]-amino}-2-phenyl 25 acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(2-cyano-biphenyl-4-ylmethyl)-amilo]-2-phelyl acetamide; (R)-2-[(4-Chloro-benzenesufony)-(4-methyl-benzyl)-amino-2-phenyl-acetaide; (R)-2-[(4-Chloro-benzenesulfonyl )-(3-nitro-benzyl)-amino]-2-phenyl-acetamide; 30 (R)-2-[(4-Chloro-benzenesulfonyl )-(2-methoxy-5-nitro-benzyi)-amino]-2-phenyl acetamide; (R)-2-[(4-Chloro-benzenesulfonyl )-(2-hydroxy-5-nitro-benzyl)-am ino]-2-phenyl acetam ide; (R)-2-[(4-Chloro-benzenesulfonyl )-(4-nitro-benzyl )-amino]-2-phenyl-acetamide; 35 (R)-2-[(4-Chloro-benzenesulfonyl )-(2,5-difluoro-benzy )-am ino]-2-phenyl-acetamide; (R)-2-[(4-Bromo-2-fluoro-benzyl)-(4-chloro-benzenesulfonyl)-amino]-2-pheny acetamide; WO 2005/095334 PCT/1B2005/000796 -14 (R)-2-[(4-Chloro-benzenesufonyl)-(4-fuoro-2-trifuoromethyI-belI)-aminoj-2-phenyl acetamide; (R)-2-[(4-Chloro-benzenesufony)-(3trifluoromethoxy-benflZY)-amfilo]-2-phel acetamide; 5 (R)-2-[(4-Chloro-benzenesufony)-(4,5-diethoxy-2-litro-belzyl)-amliflo]-2-phenl acetamide; (R)-2-[(4-Chloro-benzenesufonyl)-(3-methoxy-bely)-amil-2-phel-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(3,5-d imethoxy-benzyl)-am ino]-2-phenyl acetamide; 10 (R)-2-[(4-Chloro-benzenesufony)-(4-trifuoromethylsufal-belY)-am ino]-2-phenyl acetamide; (R)-2-[(4-Benzoyl-benzyI)-(4-chloro-benzelfofl)-amiflo]-2-phefl-acetamide; (R--(-uoy--rfurmthlqioI--ymty)(-horo-benzenesulfonyl) amino]-2-phenyl-acetamide; 15 (R)-2-[(4ChorobenzenesulfonyI)-(5,5,8,8-tetramlethyI-5,6,7,8-tetrahydro-flaphthalel 2-ylmethyl)-amino]-2-phenyI-acetamide; (R)-2-[(3-Benzyloxy-benzyl)-(4-choro-belzelesulfoflyl)-afllino]-2-phenyl-acetam ide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-pyrazo-1 -yl-benzyl)-aminol-2-phenyI acetamide; 20 (R)-2-[(4-Chloro-benzenesufonyI)-(4-ethyI-benlZI)-amino]-2-phenyl-acetamide; (R)-N-.Buty-4-{[(carbamoyl-pheny-methy)-(4-chloro-belzelesufafl)-amilo] methyl}-benzam ide; (R)-4-{[(Carbamoy-phenyl-methy)-(4-Choro-belzeeleUfonl)-amifl-methyI}-N methyl-benzamide; 25 (R)-(4-{[(Carbamoyl-phenyi-methy)-(4-choro-belzeesufoyl)-amilo]-methyI} phenyi)-acetic acid 2-oxo-2-phenyi-ethyl ester; (R)-2-[(3,5-Bis-trifluoromethy-belzy)-(4-Choro-belzeesufofl)-amilo]-2-phel acetamide; (R)-2-[(4-BenzoyI-benzyI)-(4-choro-belzeelfofl)-amiflo]-2-phefl-acetam ide; 30 (R)-2-{(4-Chloro-benzenesulfony)-[3-(2-fluoro-pheloxy)-belY-amifloP2-pheyl acetamide; (R)-2-[(4-Choro-benzenesulfonyI)-(4-chloro-benly)-amiflo]-2-phefl-acetamide; (R)-2-[(4-Chioro-benzenesulfonyI)-(3,5-dimethyl-bely)-ailo]-2-phenl-acetamide; and 35 (R)-2-[(4-Chloro-benzenesulfony)-(4-fluoro-3-trifluoromethyI-beflY)-amiflo]2-phel acetamide.
WO 2005/095334 PCT/IB2005/000796 -15 Specific embodiments of the present invention also include the following compounds of Formula I, all pharmaceutically acceptable salts thereof, complexes thereof, and derivatives thereof that convert into a pharmaceutically active compound upon administration: (R)-2-[(4-Chloro-benzenesulfonyl)-(4-morphoin-4-ylmethyl-benzyl)-am ino]-N-methyl 5 2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-morpholin-4-ylmethyl-benzyl)-amino]-N-ethyl-2 phenyl-acetamide; (R)-4-{[(4-Chloro-benzenesulfonyl)-(methylcarbamoyl-phenyl-methyl)-amino]-methyl} N-methyl-benzamide; 10 (R)-4-{[(4-Chloro-benzenesulfonyl)-(ethylcarbamoyl-phenyl-methyl)-amino]-methyl}-N methyl-benzamide; (R)-2-[(4-Bromo-2-fluoro-benzyl)-(4-chloro-benzenesulfonyl)-am ino]-N-methyl-2 phenyl-acetamide; (R)-2-[(4-Bromo-2-fluoro-benzyl)-(4-chloro-benzenesulfonyl)-amino]-N-ethyl-2-phenyl 15 acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-oxazol-2-yl-benzyl)-amino]-N-methyl-2-phenyl acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-oxazol-2-yl-benzyl)-amino]-N-ethyl-2-phenyl acetamide; 20 (R)-2-[(4-Chloro-benzenesulfonyl)-(4-chloro-2-fluoro-benzyl)-aminol-2-phenyl acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-chloro-2-fluoro-benzyl)-amino]-N-methyl-2 phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-chloro-2-fluoro-benzyl)-amino]-N-ethyl-2-phenyl 25 acetamide;. (R)-4-{[(Carbamoyl-pheny-methyl)-(4-trifluoromethoxy-benzenesulfonyl)-am ino] methyl}-benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(4-cyano-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester; 30 (R)-4-{[(Carbamoyl-phenyl-methyl)-(3-chloro-4-methyl-benzenesulfonyl)-am ino] methyl}-benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(3-fluoro-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(2,3,4-trifluoro-benzenesulfonyl)-amino]-methyl} 35 benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(3,4-difluoro-benzenesulfonyl)-amino]-m ethyl} benzoic acid methyl ester; WO 2005/095334 PCT/IB2005/000796 -16 (R)-4-{[(3-Bromo-benzenesulfonyl)-(carbamoyl-phenyl-methyl)-amino-methyl} benzoic acid methyl ester; (R)-4-{[(Carbamoyl-pheny-methyl)-(toluene-4-sulfonyl)-aminol-methyl}-benzoic acid methyl ester; 5 (R)-4-{[Benzenesulfonyl-(carbamoyl-phenyl-methyl)-amino]-methyl}-benzoic acid methyl ester; (R)-4-{[(4-Bromo-benzenesulfonyl)-(carbamoyl-pheny-methyl)-amino]-methyl} benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(3-trifluoromethyl-benzenesulfonyl)-amino] 10 methyl}-benzoic acid methyl ester; (R)-4-{[(Carbamoy-phenyl-methyl)-(4-fluoro-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(4-methoxy-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester; 15 (R)-4-{{(Carbamoyl-phenyl-methyl)-(toluene-3-sulfonyl)-amino]-methyl}-benzoic acid methyl ester; R)-4-{[(Carbamoyl-phenyl-methyl)-(2-chloro-benzenesulfonyl)-amino]-methyl}-benzoic acid methyl ester; (R)-4-{[(Carbamoy-pheny-methyl)-(3-chloro-4-fluoro-benzenesulfonyl)-amino] 20 methyl}-benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(2,4-difluoro-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(3,5-difluoro-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester; 25 (R)-4-{{(Carbamoyl-phenyl-methyl)-(4-chloro-3-nitro-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(5-fluoro-2-methyl-benzenesulfonyl)-amino] methyl}-benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(2,3-dichloro-benzenesulfonyl)-amino]-methyl} 30 benzoic acid methyl ester; (R)-4-{[(4-Bromo-2-methyl-benzenesulfonyl)-(carbamoyl-phenyl-methyl)-amino] methyl}-benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(2-trifluoromethoxy-benzenesulfonyl)-amino] methyl}-benzoic acid methyl ester; 35 (R)-4-{[(Carbamoyl-phenyl-methyl)-(3,4-dimethoxy-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester; WO 2005/095334 PCT/IB2005/000796 -17 (R)-4-{[(Carbamoyl-phenyl-methyl)-(4-chloro-2,5-dimethyl-benzenesulfony)-amino] methyl}-benzoic acid methyl ester; (R)-4-([(Carbamoyl-pheny-methyl)-(4-isopropyl-benzenesulfonyl)-amino]-m ethyl} benzoic acid methyl ester; 5 (R)-4-{[(Carbamoyl-pheny-methyl)-(4-propyl-benzenesulfonyl)-amino-methyl} benzoic acid methyl ester; and (R)-4-{[(Carbamoyl-phenyl-methyl)-(3-nitro-benzenesulfonyl)-amino]-methyl}-benzoic acid methyl ester. As indicated, so-called 'prodrugs' of the compounds of Formula I are also within the 10 scope of the invention. Thus certain derivatives of compounds of Formula I which may have little or no pharmacological activity themselves can, when administered into or onto the body, be converted into compounds of Formula I having the desired activity, for example, by hydrolytic cleavage. Such derivatives are referred to as 'prodrugs'. Further information on the use of prodrugs may be found in Pro-drugs as Novel Delivery Systems, Vol. 14, ACS 15 Symposium Series (T. Higuchi and W. Stella) and Bioreversible Carriers in Drug Design, Pergamon Press, 1987 (Ed. E. B. Roche, American Pharmaceutical Association). Prodrugs in accordance with the invention can, for example, be produced by replacing appropriate functionalities present in the compounds of Formula I with certain moieties known to those skilled in the art as 'pro-moieties' as described, for example, in 20 Design of Prodrugs by H. Bundgaard (Elsevier, 1985). Some examples of prodrugs in accordance with the invention include, but are not limited to, (i) where the compound of Formula I contains a carboxylic acid functionality (-COOH), an ester thereof, for example, a compound wherein the hydrogen of the 25 carboxylic acid functionality of the compound of Formula (1) is replaced by (C-C 8 )alkyl; (ii) where the compound of Formula I contains an alcohol functionality (-OH), an ether thereof, for example, a compound wherein the hydrogen of the alcohol functionality of the compound of Formula I is replaced by (C-C)alkanoyloxymethyl; and (iii) where the compound of Formula I contains a primary or secondary amino 30 functionality (-NH 2 or -NHR where R # H), an amide thereof, for example, a compound wherein, as the case may be, one or both hydrogens of the amino functionality of the compound of Formula I is/are replaced by (C-Clo)alkanoyl. Further examples of replacement groups in accordance with the foregoing examples and examples of other prodrug types may be found in the aforementioned references. 35 Moreover, certain compounds of Formula I may themselves act as prodrugs of other compounds of Formula 1.
WO 2005/095334 PCT/IB2005/000796 -18 Also included within the scope of the invention are metabolites of compounds of Formula 1, that is, compounds formed in vivo upon administration of the drug. Some examples of metabolites in accordance with the invention include, but are not limited to, (i) where the compound of Formula I contains a methyl group, an hydroxymethyl 5 derivative thereof (-CH 3 -> -CH 2 OH): (ii) where the compound of Formula I contains an alkoxy group, an hydroxy derivative thereof (-OR -> -OH); (iii) where the compound of Formula I contains a tertiary amino group, a secondary amino derivative thereof (-NR 1
R
2 -> -NHR' or -NHR 2 ); 10 (iv) where the compound of Formula I contains a secondary amino group, a primary derivative thereof (-NHR -> -NH 2 ); (v) where the compound of Formula I contains a phenyl moiety, a phenol derivative thereof (-Ph -> -PhOH); and (vi) where the compound of Formula I contains an amide group, a carboxylic acid 15 derivative thereof (-CONH 2 -> COOH). Compounds of Formula I containing one or more asymmetric carbon atoms can exist as two or more stereoisomers. Where a compound of Formula I contains an alkenyl or alkenylene group, geometric cisltrans (or Z/E) isomers are possible. Where structural isomers are interconvertible via a low energy barrier, tautomeric isomerism ('tautomerism') can occur. 20 This can take the form of proton tautomerism in compounds of Formula I containing, for example, an imino, keto, or oxime group, or so-called valence tautomerism in compounds which contain an aromatic moiety. It follows that a single compound may exhibit more than one type of isomerism. Included within the scope of the present invention are all stereoisomers, geometric 25 isomers and tautomeric forms of the compounds of Formula 1, including compounds exhibiting more than one type of isomerism, and mixtures of one or more thereof. Also included are acid addition or base salts wherein the counterion is optically active, for example, d-lactate or /-lysine, or racemic, for example, di-tartrate or dl-arginine. Cisltrans isomers may be separated by conventional techniques well known to those 30 skilled in the art, for example, chromatography and fractional crystallisation. Conventional techniques for the preparation/isolation of individual enantiomers include chiral synthesis from a suitable optically pure precursor or resolution of the racemate (or the racemate of a salt or derivative) using, for example, chiral high pressure liquid chromatography (HPLC). 35 Alternatively, the racemate (or a racemic precursor) may be reacted with a suitable optically active compound, for example, an alcohol, or, in the case where the compound of Formula I contains an acidic or basic moiety, a base or acid such as 1-phenylethylamine or WO 2005/095334 PCT/IB2005/000796 -19 tartaric acid. The resulting diastereomeric mixture may be separated by chromatography and/or fractional crystallization and one or both of the diastereoisomers converted to the corresponding pure enantiomer(s) by means well known to a skilled person. Chiral compounds of the invention (and chiral precursors thereof) may be obtained in 5 enantiomerically-enriched form using chromatography, typically HPLC, on an asymmetric resin with a mobile phase consisting of a hydrocarbon, typically heptane or hexane, containing from 0 to 50% by volume of isopropanol, typically from 2% to 20%, and from 0 to 5% by volume of an alkylamine, typically 0.1% diethylamine. Concentration of the eluate affords the enriched mixture. 10 When any racemate crystallises, crystals of two different types are possible. The first type is the racemic compound (true racemate) referred to above wherein one homogeneous form of crystal is produced containing both enantiomers in equimolar amounts. The second type is the racemic mixture or conglomerate wherein two forms of crystal are produced in equimolar amounts each comprising a single enantiomer. 15 While both of the crystal forms present in a racemic mixture have identical physical properties, they may have different physical properties compared to the true racemate. Racemic mixtures may be separated by conventional techniques known to those skilled in the art - see, for example, Stereochemistry of Organic Compounds by E. L. Eliel and S. H. Wilen (Wiley, 1994). 20 The present invention includes all pharmaceutically acceptable isotopically-labelled compounds of Formula I wherein one or more atoms are replaced by atoms having the same atomic number, but an atomic mass or mass number different from the atomic mass or mass number which predominates in nature. Examples of isotopes suitable for inclusion in the compounds of the invention include, 25 but are not limited to, isotopes of hydrogen, such as 2H and 3H, carbon, such as 1C, 1C and 14C, chlorine, such as 36C, fluorine, such as 1 8 F, iodine, such as 1231 and 1251, nitrogen, such as 13 N and 1 5 N, oxygen, such as 1O, 170 and 180, phosphorus, such as 32 P, and sulphur, such as 35S. Certain isotopically-labelled compounds of Formula 1, for example, those 30 incorporating a radioactive isotope, are useful in drug and/or substrate tissue distribution studies. The radioactive isotopes tritium, i.e. 3H, and carbon-14, i.e. 1C, are particularly useful for this purpose in view of their ease of incorporation and ready means of detection. Substitution with heavier isotopes such as deuterium, i.e. 2 H, may afford certain therapeutic advantages resulting from greater metabolic stability, for example, increased in 35 vivo half-life or reduced dosage requirements, and hence may be preferred in some circumstances.
WO 2005/095334 PCT/IB2005/000796 -20 Substitution with positron emitting isotopes, such as 1C, 8F, "0 and 1N, can be useful in Positron Emission Topography (PET) studies for examining substrate receptor occupancy. Isotopically-labeled compounds of Formula I can generally be prepared by 5 conventional techniques known to those skilled in the art or by processes analogous to those described in the accompanying Examples and Preparations using an appropriate isotopically labeled reagent in place of the non-labeled reagent previously employed. Pharmaceutically acceptable solvates in accordance with the invention include those wherein the solvent of crystallization may be isotopically substituted, e.g. D 2 0, de-acetone, d 6 10 DMSO. Also within the scope of the invention are intermediate compounds of Formula 11 as hereinbefore defined, all salts, solvates and complexes thereof and all solvates and complexes of salts thereof as defined hereinbefore for compounds of Formula 1. The invention includes all polymorphs of the aforementioned species and crystal habits thereof. 15 When preparing compounds of Formula I in accordance with the invention, it is open to a person skilled in the art to routinely select the form of compound of Formula II which provides the best combination of features for this purpose. Such features include, but are not limited to, the melting point, solubility, processability and yield of the intermediate form and the resulting ease with which the product may be purified on isolation. 20 Compounds of the Formula I of this invention, and their pharmaceutically acceptable salts, have useful pharmaceutical and medicinal properties. The compounds of Formula I, and their pharmaceutically acceptable salts inhibit the production of Ap-peptide (thus, gamma-secretase activity) in mammals, including humans. Compounds of the Formula 1, and their pharmaceutically acceptable salts, are therefore able to function as therapeutic agents in 25 the treatment of the neurodegenerative and/or neurological disorders and diseases representatively enumerated below, for example Alzheimer's disease, in an afflicted mammal, including a human. The present invention also relates to a pharmaceutical composition for treating a disease or condition selected from the group consisting of Alzheimer's disease, hereditary 30 cerebral hemorrhage with amyloidosis, cerebral amyloid angiopathy, a prion-mediated disease, inclusion body myositis, stroke, multiple sclerosis, head trauma, mild cognitive impairment and Down's Syndrome in a mammal, including a human, comprising an amount of a compound of the Formula I, or a pharmaceutically acceptable salt thereof, that is effective in inhibiting Ap-peptide production, and a pharmaceutically acceptable carrier. 35 The present invention also relates to a pharmaceutical composition for treating a disease or condition selected from the group consisting of Alzheimer's disease and Down's Syndrome in a mammal, including a human, comprising an amount of a compound of the WO 2005/095334 PCT/IB2005/000796 -21 Formula I, or a pharmaceutically acceptable salt thereof, that is effective in inhibiting Ap peptide production, and a pharmaceutically acceptable carrier. The present invention also relates to a pharmaceutical composition for treating a disease or a condition selected from the group consisting of Alzheimer's disease, hereditary 5 cerebral hemorrhage with amyloidosis, cerebral amyloid angiopathy, a prion-mediated disease, inclusion body myositis, stroke, multiple sclerosis, head trauma, mild cognitive impairment and Down's Syndrome in a mammal, including a human, comprising an amount of a compound of the Formula 1, or a pharmaceutically acceptable salt thereof, that is effective in treating such disease or condition, and a pharmaceutically acceptable carrier. 10 The present invention also relates to a pharmaceutical composition for treating a disease or a condition selected from the group consisting of Alzheimer's disease and Down's Syndrome in a mammal, including a human, comprising an amount of a compound of the Formula 1, or a pharmaceutically acceptable salt thereof, that is effective in treating such disease or condition, and a pharmaceutically acceptable carrier. 15 The present invention also relates to a method of treating a disease or condition selected from Alzheimer's disease, hereditary cerebral hemorrhage with amyloidosis, cerebral amyloid angiopathy, a prion-mediated disease, inclusion body myositis, stroke, multiple sclerosis, head trauma, mild cognitive impairment and Down's Syndrome in a mammal, including a human, comprising administering to said mammal an amount of a compound of 20 the Formula 1, or a pharmaceutically acceptable salt thereof, that is effective in inhibiting Ap production. The present invention also relates to a method of treating a disease or condition selected from Alzheimer's disease and Down's Syndrome in a mammal, including a human, comprising administering to said mammal an amount of a compound of the Formula 1, or a 25 pharmaceutically acceptable salt thereof, that is effective in inhibiting Ap-production. The present invention also relates to a method of treating a disease or condition selected from Alzheimer's disease, hereditary cerebral hemorrhage with amyloidosis, cerebral amyloid angiopathy, a prion-mediated disease, inclusion body myositis, stroke, multiple sclerosis, head trauma, mild cognitive impairment and Down's Syndrome in a mammal, 30 including a human, comprising administering to said mammal an amount of a compound of the Formula 1, or a pharmaceutically acceptable salt thereof, that is effective in treating such condition. The present invention also relates to a method of treating a disease or condition selected from Alzheimer's disease and Down's Syndrome in a mammal, including a human, 35 comprising administering to said mammal an amount of a compound of the Formula 1, or a pharmaceutically acceptable salt thereof, that is effective in treating such condition.
WO 2005/095334 PCT/IB2005/000796 -22 The compounds of Formula I may be used alone or used in combination with any other drug, including, but not limited to, any memory enhancement agent, e.g., AriceptTM and/or NamendaTM, antidepressant agent, e.g., ZoloftTM, anxiolytic, antipsychotic agent, e.g., GeodonTM, sleep disorder agent, anti-inflammatory agent, e.g., CelebrexTM, BextraTM, etc., anti 5 oxidant agent, cholesterol modulating agent (for example, an agent that lowers LDL or increases HDL), e.g., LipitorTM, CaduetTM, etc., Histamine (H2) antagonist, e.g., CimetadineTM, and anti-hypertension agent, e.g., NorvascTM, CaduetTM, etc. Accordingly, the present invention also relates to the following pharmaceutical compositions and methods of treatment comprising a compound of the Formula I in combination with other drugs, such as those of the 10 type described above. The present invention also relates to a pharmaceutical composition for treating a disease or condition associated with Ap-peptide production in a mammal, including a human, comprising (a) a compound of the Formula 1, or a pharmaceutically acceptable salt thereof; (b) a memory enhancement agent, e.g., AriceptTM and/or NamendaTM, antidepressant, e.g., 15 ZoloftTM, anxiolytic, antipsychotic agent, e.g., GeodonTM, sleep disorder agent, anti inflammatory agent, e.g., CelebrexTM, BextraTM, etc., anti-oxidant agent, cholesterol modulating agent (for example, an agent that lowers LDL or increases HDL), e.g., LipitorTM, Caduet T M , etc., Histamine (H2) antagonist, e.g., CimetadineTM, and anti-hypertensive agent, e.g., NorvascTM, CaduetTM, etc.; and (c) a pharmaceutically acceptable carrier; wherein the active 20 agents "a" and "b" above are present in amounts that render the composition effective in treating such disease or condition. The present invention also relates to a pharmaceutical composition for treating a disease or condition selected from the group consisting of Alzheimer's disease, hereditary cerebral hemorrhage with amyloidosis, cerebral amyloid angiopathy, a prion-mediated 25 disease, inclusion body myositis, stroke, multiple sclerosis, head trauma, mild cognitive impairment and Down's Syndrome, in a mammal, including a human, comprising (a) a compound of the Formula I, or a pharmaceutically acceptable salt thereof; (b) a memory enhancement agent, e.g., AriceptTM and/or NamendaTM, antidepressant, e.g., ZoloftTM, anxiolytic, antipsychotic agent, e.g., GeodonTM, sleep disorder agent, anti-inflammatory agent, 30 e.g., CelebrexTM, BextraTM, etc., anti-oxidant agent, cholesterol modulating agent (for example, an agent that lowers LDL or increases HDL), e.g., LipitorTM, CaduetTM, etc., Histamine (H2) antagonist, e.g., CimetadineTM, and anti-hypertensive agent, e.g., NorvascTM, CaduetTM, etc.; and (c) a pharmaceutically acceptable carrier; wherein the active agents "a" and "b" above are present in amounts that render the composition effective in treating such disease or condition. 35 The present invention also relates to a pharmaceutical composition for treating a disease or condition selected from the group consisting of Alzheimer's disease and Down's WO 2005/095334 PCT/IB2005/000796 -23 Syndrome, in a mammal, including a human, comprising (a) a compound of the Formula I, or a pharmaceutically acceptable salt thereof; (b) a memory enhancement agent, e.g., Aricept T M and/or NamendaTM, antidepressant, e.g., ZoloftTM, anxiolytic, antipsychotic agent, e.g., GeodonTM, sleep disorder agent, anti-inflammatory agent, e.g., CelebrexTM, BextraTm, etc., anti 5 oxidant agent, cholesterol modulating agent (for example, an agent that lowers LDL or increases HDL), e.g., LipitorTM, CaduetTM, etc., Histamine (H2) antagonist, e.g., CimetadineM, and anti-hypertensive agent, e.g., NorvascTM, CaduetTM, etc.; and (c) a pharmaceutically acceptable carrier; wherein the active agents "a" and "b" above are present in amounts that render the composition effective in treating such disease or condition. 10 The present invention also relates to a method of treating a disease or condition associated with Ap-peptide production in a mammal, including a human, comprising administering to said mammal (a) a compound of the Formula 1, or a pharmaceutically acceptable salt thereof; and (b) a memory enhancement agent, e.g., AriceptTM and/or NamendaTM, antidepressant, e.g., ZooftTM, anxiolytic, antipsychotic agent, e.g., GeodonTM, 15 sleep disorder agent, anti-inflammatory agent, e.g., CelebrexTM, BextraTM, etc., anti-oxidant agent, cholesterol modulating agent (for example, an agent that lowers LDL or increases HDL), e.g., Lipitor T M , Caduet T M , etc., Histamine (H2) antagonist, e.g., CimetadineTM, and anti hypertensive agent, e.g., NorvascTM, CaduetTM, etc.; wherein the active agents "a" and "b" above are present in amounts that render the composition effective in treating such disease 20 or condition. The present invention also relates to a method of treating a disease or condition selected from the group consisting of Alzheimer's disease, hereditary cerebral hemorrhage with amyloidosis, cerebral amyloid angiopathy, a prion-mediated disease, inclusion body myositis, stroke, multiple sclerosis, head trauma, mild cognitive impairment and Down's 25 Syndrome, in a mammal, including a human, comprising administering to said mammal (a) a compound of the Formula I, or a pharmaceutically acceptable salt thereof; and (b) a memory enhancement agent, e.g., AriceptTM and/or NamendaTM, antidepressant, e.g., ZoloftTM, anxiolytic, antipsychotic agent, e.g., GeodonTM, sleep disorder agent, anti-inflammatory agent, e.g., CelebrexTM, BextraTM, etc., anti-oxidant agent, cholesterol modulating agent (for example, 30 an agent that lowers LDL or increases HDL), e.g., LipitorTM, Caduet
TM
, etc., Histamine (H2) antagonist, e.g., CimetadineTM, and anti-hypertensive agent, e.g., NorvascTM, CaduetTM, etc.; wherein the active agents "a" and "b" above are present in amounts that render the composition effective in treating such disease or condition. The present invention also relates to a method of treating a disease or condition 35 selected from the group consisting of Alzheimer's disease and Down's Syndrome, in a mammal, including a human, comprising administering to said mammal (a) a compound of the WO 2005/095334 PCT/IB2005/000796 -24 Formula I, or a pharmaceutically acceptable salt thereof; and (b) a memory enhancement agent, e.g., AriceptTM and/or NamendaTM, antidepressant, e.g., ZoloftTM, anxiolytic, antipsychotic agent, e.g., GeodonTM, sleep disorder agent, anti-inflammatory agent, e.g., CelebrexTM, BextraTM, etc., anti-oxidant agent, cholesterol modulating agent (for example, an 5 agent that lowers LDL or increases HDL), e.g., Lipitor T M , Caduet T M , etc., Histamine (H2) antagonist, e.g., CimetadineTM, and anti-hypertensive agent, e.g., NovascTM; Caduetrm, etc.; wherein the active agents "a" and "b" above are present in amounts that render the composition effective in treating such disease or condition. Compounds of the Formula 1, or any of the combinations described in the immediately 10 preceding paragraphs, may optionally be used in conjunction with a known P-glycoprotein inhibitor, such as verapamil. References herein to diseases and conditions "associated with Ap-peptide production" relate to diseases or conditions that are caused, at least in part, by Ap-peptide and/or the production thereof. Thus, AP-peptide is a contributing factor, but not necessarily 15 the only contributing factor, to "a disease or condition associated with Ap-peptide production." The compounds of Formula I, and their pharmaceutically acceptable salts may also be used to modulate or inhibit the Notch signaling pathway in organisms, including humans. The Notch signaling pathway is an evolutionarily conserved mechanism utilized by organisms, ranging from worms through humans, to regulate fate determination of various cell lineages. 20 Notch belongs to the family of epidermal growth factor-like homeotic genes, which encode transmembrane proteins with variable numbers of epidermal growth factor-like repeats in the extracellular domain. There is increasing evidence for a role of the Notch pathway in human disease. All of the components of the pathway have yet to be identified, but among those identified to date, mutations that affect their interaction with each other can lead to a variety of 25 syndromes and pathological conditions. For example, Notch signaling is typically associated with cell fate decision. The finding that Notch activation stimulates capillary outgrowth suggests that Notch receptors must be activated to allow this, process to occur. Therefore, Notch modulation provides a method for regulating angiogenesis. Specifically, modulation of Notch signaling can be used 30 to modulate angiogenesis (e.g., by blocking Notch signaling to block angiogenesis). This inhibition of angiogenesis in vivo can be used as a therapeutic means to treat a variety of diseases, including but not limited to cancer, diabetic retinopathy, rheumatoid arthritis, psoriasis, inflammatory bowel disease and arteriosclerosis. The Notch pathway is also implicated in the development and maturation of T cells, 35 as described in Radtke, F. et al., Immunity 10:547-558, 1999. The compounds of Formula 1, and their pharmaceutically acceptable salts are therefore useful candidates for modulating the WO 2005/095334 PCT/IB2005/000796 -25 immune system, including the treatment of inflamamation, asthma, graft rejection, graft versus host disease, autoimmune disease and transplant rejection. In addition,. a number of studies published between 2002 and 2004 have provided convincing evidence that Notch signaling is frequently elevated in a variety of human tumors 5 (including, but not limited to breast, prostate, pancreas and T-cell acute lymphoblastic leukemia). One key study provides a strong genetic link to Notch's role in important tumor types. Specifically, Weijzen et al. demonstrated that Notch signaling maintains the neoplastic phenotype in human Ras-transformed cells. Weijzen et al. (2002) Nature Med 8: 979. Because 30% of human malignancies may carry activating mutations in at least one of the 10 three isoforms of Ras, this finding raises the possibility that Notch inhibitors would be a powerful addition to anti-cancer therapy. Another study's findings support a central role for aberrant Notch signaling in the pathogenesis of human T cell acute lymphoblastic leukemia/lymphoma. Pear et al., Current Opinion in Hematology (2004), 11(6), 426-433. Accordingly, the compounds of Formula I, and their pharmaceutically acceptable 15 salts, may be used for treating a disease or condition selected from the group consisting of cancer, arteriosclerosis, diabetic retinopathy, rheumatoid arthritis, psoriasis, inflammatory bowel disease inflammation, asthma, graft rejection, graft versus host disease, autoimmune disease and transplant rejection. As used herein, the term "treating" refers to reversing, alleviating or inhibiting the 20 progress of a disease, disorder or condition, or one or more symptoms of such disease, disorder or condition, to which such term applies. As used herein, "treating" may also refer to decreasing the probability or incidence of the occurrence of a disease, disorder or condition in a mammal as compared to an untreated control population, or as compared to the same mammal prior to treatment. For example, as used herein, "treating" may refer to preventing a 25 disease, disorder or condition, and may include delaying or preventing the onset of a disease, disorder or condition, or delaying or preventing the symptoms associated with a disease, disorder or condition. As used herein, "treating" may also refer to reducing the severity of a disease, disorder or condition or symptoms associated with such disease, disorder or condition prior to a mammal's affliction with the disease, disorder or condition. Such 30 prevention or reduction of the severity of a disease, disorder or condition prior to affliction relates to the administration of the composition of the present invention, as described herein, to a subject that is not at the time of administration afflicted with the disease, disorder or condition. As used herein "treating" may also refer to preventing the recurrence of a disease, disorder or condition or of one or more symptoms associated with such disease, disorder or 35 condition. The terms "treatment' and therapeuticallyy," as used herein, refer to the act of treating, as "treating" is defined above.
WO 2005/095334 PCT/IB2005/000796 -26 Detailed Description of the Invention Compounds of the Formula I, and their pharmaceutically acceptable salts, may be prepared as described in the following reaction Schemes and discussion. Unless otherwise indicated, as referred to in the reaction schemes and discussion that follow, R 1 , R 2 , R 3 , R 5 , R 6 , 5 R 7 , R, R 9 , A, m, n and q are as defined above. The compounds of Formula I may have asymmetric carbon atoms and may therefore exist as racemic mixtures, diastereoisomers, or as individual optical isomers. Separation of a mixture of isomers of compounds of Formula I into single isomers may be accomplished according to conventional methods known in the art. 10 The compounds of the Formula I may be prepared by the methods described below, together with synthetic methods known in the art of organic chemistry, or modifications and derivatisations that are familiar to those of ordinary skill in the art. Preferred methods include, but are not limited to, those described below. The reactions described below are performed in solvents that are appropriate to the 15 reagents and materials employed and that are suitable for use in the reactions described. In the description of the synthetic methods described below, it is also to be understood that all reaction conditions, whether actual or proposed, including choice of solvent, reaction temperature, reaction duration time, reaction pressure, and other reaction conditions (such as anhydrous conditions, under argon, under nitrogen, etc.), and work up procedures, are those 20 conditions that are standard for that reaction, as would be readily recognized by one of skill in the art. Alternate methods may also be used.
WO 2005/095334 PCT/IB2005/000796 -27 Scheme I O (R1)m 0 H2N NH2CI (R1)m~ OjNH _O 0
NH
2 H.
H
2 N III NH, 00 II (R2)n V 2) IV 2)n n = 0,1
R
3 OH OR
R
3 X R 3 (R1)ma 0 NH 1/ NH 2 0 ) ( 2)nA Scheme 1 illustrates methods suitable for preparing sulfonamide compounds of formula 1. The amide derivatives |1 are available commerically as racemic mixtures or single 5 enantiomers or prepared by methods known in the chemical literature such as Chem.Ber. 1939; 72, 1291; J.Chem.Soc. 1911, 99, 323; J.Org.Chem., 1962, 27, 798; J.Org.Chem. 1962, 27, 798; Bull.Soc.Chim.Be/g. 1997, 106, 67; Chem.Pharm.Bu//. 2000, 48, 1586; J.Org.Chem. 2002, 67, 3687; J.Med.Chem. 2002, 45, 5471; Collect.Czech.Chem.Commun. 1995, 60, 150; J.Chem.Soc.C. 1968; 531; Tetrahedron Lett. 1992, 33, 6007. The amide derivatives |1 are 10 reacted with a sulfonyl chloride III in a solvent such as methylene chloride, dichloroethane, toluene, benzene, or ether in which methylene chloride is preferred at a temperature from 500C to 50 0C in which -10 0C to 23 0C is preferred to afford sulfonamides IV. The sulfonamides IV are treated with a base such as potassium carbonate, sodium carbonate, alkoxide, alkyllithiate in which potassium carbonate is preferred and R 3 -X where X is defined 15 as bromide, iodide, fluoride, chloride, alkylsulfonate or arylsulfonate at a temperature from 23 0C to 100 "C where 40 0C to 60 0C is preferred in a solvent such as methylene chloride, dimethylsulfoxide, dimethylformamide, tetrahydrofuran, acetonitrile, or toluene where dimethylformamide is preferred affords compounds of Formula 1. Alternatively, sulfonamide IV is treated with triarylphospine such as triphenyiphospine, a dialkylazodicaroxylate, and R3_ 20 OH in a solvent such as tetrahydrofuran, methylene chloride, or toluene wherein tetrahydrofuran is preferred at a temperature from 0 *C to 100 0C wherein 23 C c to 50 0C is preferred affords compounds of Formula 1.
WO 2005/095334 PCT/IB2005/000796 -28 Scheme 2 0 (RI)m O 0 (R1)m
(R
1 ) 0
H
2 N H~ . . H O ~ I OMe S, OH v Step1 Step 2 (R2)n (R2, Step 3
R
3 0H (R1)- oo (R1)m~R r H 0Io _________'- N
R
3 X R3
NH
2 S NH 2 Step 4 "0 Step 5 villa
(R
2 )n
(R
2 )n 5 Scheme 2 illustrates methods suitable for preparing sulfonamide compounds of formula I. The amide derivatives V are available commerically as racemic mixtures or single enantiomers or prepared by methods previously reported in the chemical literature. In step 1, the amide derivatives V are reacted with a sulfonyl chloride III in a solvent such as methylene chloride, dichloroethane, toluene, benzene, or ether in which methylene chloride is preferred 10 at a temperature from -50'C to 50 'C in which -10 0C to 23 "C is preferred affords sulfonamides VI. In step 2, the ester is hydrolyzed using sodium or lithium hydroxide to afford the acid VIl. In step 4, the acid is converted to amide Vill by converting the acid into a leaving group by reaction with oxalyl chloride, thionyl chloride or a mixed anhydride method, using an alkyl chloroformate, such as C1-C4 alkyl chloroformate, in the presence of a base such as 15 triethylamine, N,N-diisopropylethylamine, pyridine, or dimethylaminopyridine, in a suitable solvent such as, for example, methylene chloride, chloroform, tetrahydrofuran (THF), toluene, diethyl ether, acetonitrile, 1,4-dioxane, N,N-dimethylformamide, dimethylsulfoxide (DMSO), N methyl pyrrolidinone (NMP), or xylene, at a temperature of from about -30'C to about room temperature followed by the addition of an amine at a temperature from about 00C to 100 0 C, 20 preferably 200C. In step 3, the ester VI can be converted to the amide Vill using methods well known in the chemical literature such as treatment with ammonia in an alcoholic solvent such as methanol with heating from 50 *C to 75 C to afford the am ides Vill. Alternatively, ester VI can be converted to the amide Vill in the presence of an amine which has been treated with trialkylaluminium preferably trimethylaluminum in an appropriate solvent such as methylene 25 chloride, THF, dioxane, toluene, etc., at an appropriate temperature, such as from about room temperature to about reflux, or in a sealed reactor (such as sealed tube or inscrewed vials). The sulfonamides Vill are treated with a base such as potassium carbonate, sodium WO 2005/095334 PCT/IB2005/000796 -29 carbonate, alkoxide, alkyllithiate in which potassium carbonate is preferred and R 3 -X where X is defined as bromide, iodide, fluoride, chloride, alkylsulfonate or arylsulfonate at a temperature from 23 0C to 100 0C where 40 0C to 60 0C is preferred in a solvent such as methylene chloride, dimethylsulfoxide, dimethylformamide, tetrahydrofuran, acetonitrile, 5 toluene where dimethylformamide is preferred affords compounds of Formula 1. Alternatively, sulfonamide Vill is treated with triarylphospine such as triphenylphospine, a dialkylazodicaroxylate, and R 3 -OH in a solvent such as tetrahydrofuran, methylene chloride, toluene wherein tetrahydrofuran is preferred at a temperature from 0 C to 100 0C wherein 23 0C to 500C is preferred affords compounds of Formula 1. 10 Scheme 3 N0 0 3 (Ri) ra R 3 0
H
2 N OROH N N NH2
NH
2 C0 R O NH 2
R
3 X lII or x (~K R 3 CHO X (R ( Scheme 3 illustrates methods suitable for preparing sulfonamide compounds of formula 1. The amide derivatives IX are available commerically as racemic mixtures or single 15 enantiomers or prepared by methods previously reported in the chemical literature from amines 11. The amines IX are treated with a base such as potassium carbonate, sodium carbonate, alkoxide, alkyllithiate in which potassium carbonate is preferred and Ra-X where X is defined as bromide, iodide, fluoride, chloride, alkylsulfonate or arylsulfonate at a temperature from 23 0C to 100 0C where 40 *C to 60 0C is preferred in a solvent such as 20 methylene chloride, dimethylsulfoxide, dimethylformamide, tetrahydrofuran, acetonitrile, toluene where dimethylformamide is preferred affords compounds of Formula X. Alternatively, amines IX is treated with triarylphospine such as triphenylphospine, a dialkylazodicaroxylate, and R-OH in a solvent such as tetrahydrofuran, methlyene chloride, toluene wherein tetrahydrofuran is preferred at a temperature from 0 0C to 100 0C wherein 23 25 OC c to 50 0C is preferred affords compounds of Formula X. Alternatively, amine IX is converted to X using a well-established reductive amination method by reacting amine IX with a ketone or aldehyde with or without acid catalyst/ammonium acetate/dry agents (such as anhydrous Na 2
SO
4 or MgSO 4 ), and a reducing agent, such as sodium triacetoxyborohydride, sodium cyanoborohydride, or sodium borohydride, or the corresponding polymer bound 30 NaBH 4 , polymer bound-NaBH 3 CN, or polymer bound-NaB(OAc) 3 H, or any reducing agent (e.g., hydrogenation) that is known in the literature for reducing an imine bond to an amine, in an appropriate solvent, such as dichloroethane, chloroform, THF, MeOH, ethanol, isopropanol, t-butanol or toluene, at a temperature from about room temperature to about WO 2005/095334 PCT/IB2005/000796 -30 reflux, preferably from about room temperature to about 650C. The derivatives X are reacted with a sulfonyl chloride III in a solvent such as methylene chloride, dichloroethane, toluene, benzene, ether, dimethylformamide in which dichloroethane is preferred at a temperature from -50 0 C to 100 0C in which 20 C to 80*C is preferred affords compounds of Formula 1. 5 Scheme 4 0 0 H 0 X OH NH3 X NH 2
R
3 NH2 R.N NH 2 (1R (11R X (R2 ( 2)n R 2 On Scheme 4 illustrates methods suitable for preparing intermediate X which affords 10 sulfonamide compounds of formula I using methods described above. The acid derivatives XI are available commerically as racemic mixtures or single enantiomers or are prepared by methods well known in the chemical literature. The amides XII are formed from acid XI using methods well known in the literature (Cabre, J. Synthesis, 5; 1984, 413-417). Treatment of XII (where X is halide or aryl sulfonate) with an appropriate amine (R 3
NH
2 ) at a temperature 15 from 23 C to 100 C where 60 0C to 800C is preferred neat or in a solvent such as methylene chloride, dimethylsulfoxide, dimethylformamide, tetrahydrofuran, acetonitrile, toluene where neat is preferred affords compounds of Formula X. Alternatively, treatment of XII (where X is OH) with a triarylphospine such as triphenylphospine, a dialkylazodicaroxylate, and R 3
-NH
2 in a solvent such as tetrahydrofuran, methylene chloride, toluene wherein tetrahydrofuran is 20 preferred at a temperature from 00C to 100 0C wherein 230C to 500C is preferred affords compounds of Formula X. The starting materials used in the procedures of the Schemes described above, the syntheses of which are not described above, are either commercially available, known in the art or readily obtainable from known compounds using methods that will be apparent to those 25 skilled in the art. The compounds of Formula I, and the intermediates shown in the above reaction schemes, may be isolated and purified by conventional procedures, such as recrystallization or chromatographic separation, such as on silica gel, either with an ethyl acetate/hexane elution gradient, a methylene chloride/methanol elution gradient, or a chloroform/methanol 30 elution gradient. Alternatively, a reverse phase preparative HPLC or chiral HPLC separation technique may be used. In each of the reactions discussed or illustrated above, pressure is not critical unless otherwise indicated. Pressures from about 0.5 atmospheres to about 5 atmospheres are WO 2005/095334 PCT/IB2005/000796 -31 generally acceptable, and ambient pressure, i.e., about 1 atmosphere, is preferred as a matter of convenience. A compound of the Formula I of the present invention, or pharamaceutically acceptable salt thereof, may be administered to mammals via either the oral, parenteral (such as 5 subcutaneous, intravenous, intramuscular, intrasternal and infusion techniques), rectal, intranasal, topical or transdermal (e.g., through the use of a patch) routes. In general, these compounds are most desirably administered in doses ranging from about 0.1 mg to about 1000 mg per day, in single or divided doses (i.e., from 1 to 4 doses per day), although variations will necessarily occur depending upon the species, weight, age and condition of the subject being 10 treated, as well as the particular route of administration chosen. However, a dosage level that is in the range of about 0.1 mg/kg to about 5 gm/kg body weight per day, preferably from about 0.1 mg/kg to about 100 mg/kg body weight per day, is most desirably employed. Nevertheless, variations may occur depending upon the species of animal being treated and its individual response to said medicament, as well as on the type of pharmaceutical formulation chosen and 15 the time period and interval at which such administration is carried out. In some instances, dosage levels below the lower limit of the aforesaid range may be more than adequate, while in other cases still larger doses may be employed without causing any harmful side effects, provided that such higher dosage levels are first divided into several small doses for administration throughout the day. Varations based on the aforementioned dosage range may 20 be made by a physicain of ordinary skill. A compound of the Formula I of the present invention may be administered alone or in combination with pharmaceutically acceptable carriers or diluents by either of the routes previously indicated, and such administration may be carried out in single or multiple doses. Suitable pharmaceutical carriers include solid diluents or fillers, sterile aqueous media and 25 various non-toxic organic solvents, etc. The pharmaceutical compositions formed by combining a compound of the Formula 1, or a pharmaceutically acceptable salt thereof, with a pharmaceutically acceptable inert carrier, can then be readily administered in a variety of dosage forms such as tablets, capsules, lozenges, troches, hard candies, powders, sprays, creams, salves, suppositories, jellies, gels, pastes, lotions, ointments, aqueous suspensions, 30 injectable solutions, elixirs, syrups, and the like. Moreover, oral pharmaceutical compositions may be suitably sweetened and/or flavored. For oral administration, tablets containing various excipients such as microcrystalline cellulose, sodium citrate, calcium carbonate, dicalcium phosphate and glycine may be employed along with various disintegrants such as starch (preferably corn, potato or tapioca 35 starch), methylcellulose, alginic acid and certain complex silicates, together with granulation binders such as polyvinylpyrrolidone, sucrose, gelatin and acacia. Additionally, lubricating agents such as magnesium stearate, sodium lauryl sulfate and talc are often useful for WO 2005/095334 PCT/IB2005/000796 -32 tabletting purposes. Solid compositions of a similar type may also be employed as fillers in gelatin capsules. Preferred materials in this connection include lactose or milk sugar as well as high molecular weight polyethylene glycols. When aqueous suspensions and/or elixirs are desired for oral administration, the active ingredient may be combined with various 5 sweetening or flavoring agents, coloring matter or dyes, and, if so desired, emulsifying and/or suspending agents as well, together with such diluents as water, ethanol, propylene glycol, glycerin and various like combinations thereof. For parenteral administration, solutions containing a compound of the Formula I of the present invention or a pharmaceutically acceptable salt thereof in either sesame or peanut 10 oil, in aqueous propylene glycol or in sterile aqueous solutions may be employed. The aqueous solutions should be suitably buffered (preferably pH greater than 8) if necessary and the liquid diluent first rendered isotonic with sufficient saline or glucose. These aqueous solutions are suitable for intravenous injection purposes. The oily solutions are suitable for intraarticular, intramuscular and subcutaneous injection purposes. The preparation of all 15 these solutions under sterile conditions is readily accomplished by standard pharmaceutical techniques well known to those skilled in the art. The compounds of Formula I of the present invention are useful in inhibiting Ap peptide production (thus, gamma-secretase activity) in mammals, and therefore they are able to function as therapeutic agents in the treatment of the aforementioned disorders and 20 diseases in an afflicted mammal. A specific compound of the Formula I can be determined to inhibit Ap-peptide production using biological assays known to those of ordinary skill in the art, for example the assays described below. The activity of compounds of the Formula I of the present invention in inhibiting 25 gamma-secretase activity was determined in a solubilized membrane preparation generally according to the description provided in McLendon et al. Cell-free assays for y-secretase activity, The FASEB Journal (Vol. 14, December 2000, pp. 2383-2386). Using such assay, compounds of the present invention were determined to have an IC5 activity for inhibiting gamma-secretase activity of less than about 100 micromolar. Preferred compounds of the 30 invention are compounds that were determined to have an IC5o activity for inhibiting gamma secretase activity of less than about 5 micromolar. The following Examples illustrate the present invention. It is to be understood, however, that the invention, as fully described herein and as recited in the claims, is not intended to be limited by the details of the following Examples.
WO 2005/095334 PCT/IB2005/000796 -33 Examples Preparation 1 2-(4-Chloro-benzenesulfonylamino)-3-phenV-propionic acid methyl ester 2-Amino-3-phenyl-propionic acid methyl ester is dissolved in 25 mL of methylene 5 chloride and the reaction charged with triethylamine (0.97 mL, 7 mmol) and 4 chlorobenzenesulfonychloride at 0 0 C. The reaction is allowed to warm to room temperature (rt) and stirred overnight. The reaction is quenched with sodium bicarbonate, extracted with methylene chloride, dried over sodium sulfate, and concentrated in vacuo. The resultant solid is triturated with 20%ethylacetate/hexanes to provide the title compound. 13C NMR ( 100 10 MHz, CDCl3) 39.5, 52.8, 57.1, 127.5, 128.8, 128.9, 129.5, 129.6, 135.1, 138.4, 139.4, 171.5. MS 354.1 m/z (M+1). (R)-Amino-(4-fluoro-phenvl)-acetic acid methyl ester (R)-Amino-(4-fluoro-phenyl)-acetic acid was treated with HCI gas (8g) dissolved in methanol at room temperature for 24 hours. The reaction mixture was concentrated and 15 aqueous sodium bicarbonate was added until the pH of the mixture remained basic. The aqueous layer was extracted three times with dichloromethane. The combined extracts were dried (Na 2
SO
4 ) and concentrated afford the title compound as an oil. IHNMR (400MHz, CDCl3) 8 1.90 (br s, 2H), 3.67 (s, 3H), 4.58 (s, 1 H), 7.00 (m, 2H), 7.30 (m, 2H). Preparation 2 20 2-(4-Chloro-benzenesulfonylamino)-3-phenvl-propionamide To 2-(4-Chloro-benzenesulfonylam ino)-3-phenyl-propionic acid methyl ester (750 mg) is added 1 OmL of a 1.OM solution of ammonia in methanol. The reaction is heated for 48h at 65"C, cooled to room temperature, and the solvent removed. The resultant solid is first triturated with ethyl acetate, filtered, and then triturated with methylene chloride to provide the 25 title compound. 1HNMR ( 400 MHz, CD30D) 2.70 (dd, IH, J = 14.0 Hz and 9.6 Hz), 3.01 (dd, IH, J = 14.0 Hz and 5.4 Hz), 3.95 (dd, 1H, J = 9.0 Hz and 5.0 Hz), 7.05-7.15 (m, 5H), 7.32-7.36 (m, 2H), and 7.53-7.56 (m, 2H). MS 339.1 m/z (M+1). (R)- 2-Amino-2-(4-fluoro-phenyi)-acetamide The title compound was prepared in the manner of Preparation 2 utilizing (R)-amino 30 (4-fluoro-phenyl)-acetic acid methyl ester to yield the desired product. 1 HNMR (400 MHz, CDCi3) 5 4.30 (s, 1 H), 5.54 (br s, 1H), 6.91 (br s,1 H), 7.05 (m, 2H), 7.40 (m, 2H).
WO 2005/095334 PCT/IB2005/000796 -34 Example 1 4-f{f(1-Carbamoyl-2-phenyl-ethyl)-(4-chloro-benzenesulfonyl)-aminol-methyl)-benzoic acid methyl ester To a solution of triphenylphosphine (11Ornmg, 0.4 mmol) in 0.7 mL of tetrahydrofuran 5 is added diisopropyl azodicarboxylate (0.08 mL, 0.4 mmol) and the reaction stirred for 30 min. To the reaction is added 0.4 mL of tetrahydrofuran, (2-(4-chloro-benzenesulfonylamino)-3 phenyl-propionamide (75 mg, 0.2 mmol), and methyl-4-hydroxybenzoate (73mg, 0.4 mmol). The reaction is allowed to warm to room temperature (rt) and stirred overnight. The solvent is removed and the resultant residue purified by silica gel chromatography to provide the title 10 compound. C13 NMR (100 MHz, CDCI3) 35.5, 48.7, 52.4, 61.4, 127.1, 128.8, 128.9, 129.0, 129.3, 129.6, 129.9, 136.8, 138.1, 139.8, 141.7, 166.9, 171.0. MS 487.1 m/z (M+1). Preparation 3 (R)-2-(4-Chloro-benzenesulfonylamino)-2-phenvl-acetamide To a solution of D-(-)-2-Amino-2-phenyl-acetamide (6.0g, 40 mmol) in 150 mL of 15 methylene chloride is added tfiethylamine (6.9 mL, 50 mmol) and 4 chlorobenzensulfonylchloride (8.43g, 40 mmol) at 0 0 C. The reaction is warmed to room temperature and stirred overnight. The reaction is quenched with sodium bicarbonate and methylene chloride is added. The solid is filtered, washed with water and ether and dried in vacuo to provide 12.3 g of the title compound. C13 NMR (100 MHz, CD30D) 60.3, 127.3, 20 128.1, 128.4, 128.7, 128.9, 137.1, 138.5, 139.7, 172.9. MS 325.1 m/z (M+1). (R)- 2-(4-chloro-benzenesulfonylamino)-2-(4-fluoro-phenyl)-acetamide The title compound was synthesized according to the procedure of Preparation 3 employing (R)- 2-amino-2-(4-fluoro-phenyl)-acetamide and 4-chlorobenzenesulfonylchloride to afford the desired product. 1HNMR (400 MHz, CD30D) 8 4.87 (s, 1H), 6.94 (m, 2H), 7.24 25 m, 2H), 7.6 (dd, 4H, J=2.1, 103). Example 2 (R)-4-{f(Carbamol-phenyl-methyl)-(4-chloro-benzenesulfonyl)-aminol-methVl}-benzoic acid methyl ester To a solution of triphenylphosphine (48mmg, 0.2 mmol) in 0.7 mL of tetrahydrofuran 30 is added diisopropyl azodicarboxylate (0.04 mL, 0.2 mmol) and the reaction stirred for 30 min. To the reaction is added 0.4 mL of tetrahydrofuran, (R)-2-(4-chloro-benzenesulfonylamino)-2 phenyl-acetamide (32 mg, 0.2 mmol), and methyl-4-hydroxymethylbenzoate (73mg, 0.2 mmol). The reaction is allowed to warm to room temperature and stirred overnight. The solvent is removed and the resultant residue purified by silica gel chromatography to provide 35 the title compound. C13 NMR (100 MHz, CDCl3) 49.9, 52.3, 64.4, 127.9, 129.0, 129.2, 129.4, 129.5, 130.1, 133.5, 139.7, 142.5, 167.0, 171.0. MS 473.1 m/z (M+1).
WO 2005/095334 PCT/IB2005/000796 -35 Exam ple 2A (R)-4-{[Carbamoyl-(4-fluoro-phenyl)-methvll-(4-chloro-benzenesulfonVl)-aminol-methyll benzoic acid methyl ester The title compound was prepared employing the procedure in Example 2 using (R) 5 2-(4-chloro-benzenesulfonylamino)-2-(4-fluoro-phenyl)-acetamide and methyl-4 hydroxymethylbenzoate to afford the desired product. Diagnostic 13CNMR (100MHz, CDCI3) 49.9, 52.3, 63.4, 116.0, 116.2, 127.9, 129.1, 129.5, 129.6, 132.0, 132.1,139.8, 142.3. MS 491.2 m/z (M+1). Example 3 10 (R)-2-[(4-Chloro-benzenesulfonyl)-(4-dimethylaminomethyl-benzl)-aminol-2-phenvl acetamide The title compound was prepared employing the procedure detailed in Example 2, using (4-dimethylaminomethyl-phenyl)-methanol to afford the desired product (10mg, 3% yield); 13CNMR (100 MHz, CDCI3) 45.3, 50.1, 64.0, 64.8, 128.5, 129.0, 129.1, 129.2, 129.3, 15 129.6, 130.2, 133.9, 135.7, 138.7, 139.4, 171.5 MS 472.1 m/z (M+1). Example 4 (R)-2-[(4-Chloro-benzenesulfonvl)-(4-morpholin-4-vlmethyl-benzl)-aminol-2-phenyl acetamide The title compound was prepared employing the procedure detailed in Example 2, 20 using (4-morpholin-4-ylmethyl-phenyl)-methano to afford the desired product (170mg, 43% yield); 13CMNR (100 MHz, CDCI3) 50.1, 53.7, 63.2, 64.8, 67.2, 128.5, 129.0, 129.1, 129.2, 129.3,130.2, 133.9, 135.7, 138.7,139.4, 171.4 MS 514.1 m/z (M+1). Example 5 (R)-2-[Benzvl-(4-chloro-benzenesulfonyl)-aminol-2-phenvl-acetamide 25 To a solution of (R)-2-(4-chloro-benzenesulfonylamino)-2-phenyl-acetamide (350 mg, 1.1 mmol) in 4 mL of dimethylformamide are added benzylbromide (0.18 mL, 1.1 mmol) and potassium carbonate (179 mg, 1.3 mmol). The reaction is heated overnight at 50 0 C, poured into water/methylene chloride and extracted with methylene chloride. The combined organics are dried with sodium sulfate, concentrated in vacuo, and the resultant residue purified on 30 silica gel chromatography to provide the title compound. C13 NMR (100 MHz, CDCI3) 50.3, 64.8, 127.6, 128.4, 128.6, 129.0, 129.1, 129.3, 129.4, 130.2, 133.9, 136.8, 138.6, 139.4, 171.7. MS 415.1 m/z (M+1). Example 6 (R)-2-[(4-Chloro-benzenesulfonvl)-(4-cyano-benzvyl)-aminol-2-phenvl-acetamide 35 The title compound was prepared in a manner analogous to that in Example 5, utilizing 4-cyanobenzyl bromide to provide the desired product in 50% yield; 13C NMR ( 100 WO 2005/095334 PCT/IB2005/000796 -36 MHz, CDCl3) 49.6, 63.8, 110.7,119.0,128.4,129.1, 129.2, 129.5, 129.6, 130.0, 131.8,133.5, 138.0, 139.8, 143.2, 170.8. MS 440.4 mIz (M+1). Example 7 (R)-2-[(4-tert-Butvl-benzvl)-(4-chloro-benzenesulfonyl)-aminol-2-phenyl-acetamide 5 The title compound was prepared as detailed in Example 5, utilizing 1-bromomethyl 4-tert-butyl-benzene to afford the desired product in 28% yield; 13CNMR (100 MHz, CDCl3) 31.5, 34.6, 50.1, 65.0, 125.3, 128.6, 129.0, 129.1, 129.2, 130.2, 133.3, 134.1, 139.2, 150.8, 171.6. MS 471.1 m/z (M+1). Example 8 10 (R)-2-[(4-Chloro-benzenesulfonvl)-(3-cvano-benzvl)-aminol-2-phenvi-acetamide The title compound was prepared as detailed in Example 5, utilizing 3-bromomethyl benzonitrile to afford the desired product in 49% yield; 13CNMR (100 MHz, CDCl3) 49.3, 63.7, 112.0, 118.9, 128.8, 129.1, 129.3, 129.5, 129.7, 130.0, 130.7, 131.6, 132.5, 133.6, 139.1, 139.8, 170.9. MS 440.0 m/z (M+1). 15 Example 9 (R)-2-[(4-Chloro-benzenesulfonyl)-(4-trifluoromethoxy-benzyl)-aminol-2-phenVl-acetamide The title compound was prepared as detailed in Example 5, utilizing 1-bromomethyl 4-trifluoromethoxy-benzene to afford the desired product in 49% yield; 13CNMR (100 MHz, CDCI3) 49.4, 64.2, 120.7, 129.0, 129.2, 129.3, 129.4, 129.8, 130.0, 133.7, 135.8, 138.5, 20 138.6,148.3,171.0. MS 499.0 m/z (M+1). Example 10 (R)-2-[(4-Chloro-benzenesulfonyl)-(4-f[,2,31thiadiazol-4-yl-benzyl)-aminol-2-phenyl-acetamide The title compound was prepared as detailed in Example 5, utilizing 4-(4 bromomethyl-phenyl)-[1,2,3]thiadiazole to afford the product in 8% yield; 13CNMR (100 MHz, 25 CDCl3) 50.0, 64.6, 127.3, 127.9, 128.7, 129.1, 129.2, 129.3, 129.4, 129.5, 129.9, 130.1, 133.8,138.5, 139.6, 162.7, 171.2. MS 499.0 m/z (M+1). Example 11 (R)-2-[(4-Chloro-benzenesulfonvl)-(4-oxazol-2-vl-benzy)-aminol-2-phenVl-acetamide The title compound was prepared as detailed in Example 5 utilizing 2-(4 30 bromomethyl-phenyl)-oxazole to afford the title compound in 4% yield; 13CNMR (100 MHz, CDCI3) 50.0, 64.5, 126.3, 126.5, 128.6, 129.2, 129.4, 129.5, 130.1, 133.6, 138.7, 139.5, 139.6, 171.1. MS 482.0 m/z (M+1).
WO 2005/095334 PCT/IB2005/000796 -37 Example 12 (R)-2-f(4-Chloro-benzenesulfonyl)-[4-(3-methyl-[1,2,41oxadiazol-5-yl)-benzvll-amino}-2 phenvl-acetamide The title compound was prepared in the manner outlined in Example 5, utilizing 5-(4 5 bromomethyl-phenyl)-3-methyl-[1,2,4]oxadiazole to afford the product in 14% yield; 13C NMR (100 MHz, CDCl3) 11.9, 49.8, 64.1, 122.9, 127.8, 128.6, 129.1, 129.2, 129.4, 129.5, 130.1, 133.5,138.2,139.7, 142.6, 168.0, 170.9, 175.4 MS 497.1 m/z (M+1). Example 13 (R)-2-f(4-Chloro-benzenesulfonyl)-f4-(2-methyl-2H-tetrazol-5-vi)-benzyll-amino}-2-phenyl 10 acetamide The title compound was prepared in the manner outlined in Example 5, utilizing 5-(4 bromomethyl-phenyl)-2-methyl-2H-tetrazole to afford the product in 5% yield; Diagnostic 13CNMR (100 MHz, CDCl3) 39.7, 50.0, 64.6, 126.4, 126.7, 127.2, 128.8, 129.2, 129.4, 130.1, 139.4, 139.6, 165.1, 171.1. 15 Example 14 (R)-2-{(4-Chloro-benzenesulfonyl)-[4-(1-hydroxv-1-methyl-ethyl)-benzyll-aminol-2-phenvl acetamide (R)-4-{[(1-Carbamoyl-2-phenyl-ethyl)-(4-chloro-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester (300 mg, 0.63mmol) was dissolved in ImL THF and the solution 20 was chiled in an ice bath. 3.0 M MeMgBr in diethyl ether (0.63ml, 1.9 mmol) was added and the reaction mixture was stirred for 45min. An additional portion of MeMgBr (0.63ml, 1.9mmol) was added and, after 30min, the reaction was quenched by slow addition of water. The quenched reaction mixture was diluted with aqueous soduim bicarbonate and extracted with dichloromethane. After drying (sodium sulfate) the combined organics were 25 concentrated to an oil. Purification on a silica gel flash column (40% EtOAc in hexanes) afforded 31mg (10% yield) of the title compound. Preparation 4 (R)-2-(2,2-Dimethyl-propylamino)-2-phenyl-acetamide D-(-)-2-Amino-2-phenyl-acetamide (1.0g, 6.7mmol) was partially dissolved in 20ml 30 dichloromethane and treated with trimethylacetaldehyde (723uL, 6.7mmol). After stirring 20 min at room temperature, sodium triacetoxyborohydride (1.77g, 8.4mmol) was added and the resulting mixture was stirred at room temperature (rt) for 18h. After diluting with aqueous sodium bicarbonate, the mixture was extracted with dichloromethane. The combined organics were dried (Na 2
SO
4 ) and concentrated to a white solid (1.43g, 97% yield). MS 221.2 35 m/z (M+1).
WO 2005/095334 PCT/IB2005/000796 -38 Example 15 (R)-2-[(4-Chloro-benzenesulfonyl)-(2,2-dimethyl-propyl)-aminol-2-phenyl-acetamide (R)-2-(2,2-Dimethyl-propylamino)-2-phenyl-acetamide ( 7 0 0mg, 3.2mmol) was dissolved in dichloromethane (15mL) and chilled in an ice bath. The solution was treated with 5 triethylamine (553uL, 4.Ommol) followed by 4-chlorobenzenesulfonyl chloride (671mg, 3.2mmol). After heating at 500 C for 18h, the mixture was cooled, diluted with aqueous sodium bicarbonate and extracted with dichloromethane. The combined organic extracts were dried (Na 2
SO
4 ) and concentrated to an oil. Purification on a silica gel flash column (40%EtOAc in hexanes, eluent) afforded the title compound in 3% yield. 13CNMR (100 MHz, 10 CDCI3) 28.6, 33.2, 62.7, 70.0, 128.8, 129.0, 121.1, 129.2, 130.7, 133.6, 138.7, 139.0, 172.7. MS 395.1 m/z (M+1). Examples 16-77 (R)-2-(4-Chloro-benzenesulfonylamino)-3-phenyl-propionamide (24.3mg, 0.075mmol) was dissolved in 0.41 ml DMF. The resulting solution was cooled to 00 C and 1 N potassium 15 t-butoxide in THF (0.09 ml, 0.09mmol) was added. After warming to room temperature, the solution was added to a benzyl bromide (0.075mmol) that is substituted as indicated in the respective definitions of R 3 in the Examples recited in Table 1 below, and the reaction mixture was heated at 60 0 C overnight. After cooling and diluting with water, the organics were extracted with dichloromethane, dried with sodium sulfate and concentrated. The samples 20 were purified on a Waters Xterra PrepMS C 1 8 column (5uM, 30 X 100 mm) eluting with 0.1% TFA in water and acetonitrile in a gradient system. Examples 16-77 in Table 1 were synthesized by methods analogous to those described above. Table 1 Ex Theo. Obs. MS HPLC Name Mass m/z (M+1) RT (min) 16 428.1 429.0 2.38 (R)-2-[(4-Chloro-benzenesulfonyl)-(2 methyl-benzyl)-amino]-2-phenyl acetamide 17 428.1 429.0 2.46 (R)-2-[(4-Chloro-benzenesulfonyl)-(3 methyl-benzyl)-amino]-2-phenyl acetamide 18 448.0 449.0 2.49 (R)-2-[(4-Chloro-benzenesulfonyl)-(3 chloro-benzyl)-amino]-2-phenyl acetamide 19 439.1 440.0 2.31 (R)-2-[(4-Chloro-benzenesulfonyl)-(2- WO 2005/095334 PCT/1B2005/000796 -39 Ex Theo. Obs. MS HPLC Name Mass m/z (M+1) RT (min) cyano-benzyl)-amino]-2-phenyl-acetamide 20 432.0 433.0 2.39 (R)-2-[(4-Chloro-benzenesulfonyl)-(3 fluoro-benzyl)-amino]-2-pheny!-acetamide 21 432.0 433.0 2.39 (R)-2-[(4-Chloro-benzenesulfonyl)-(4 fluoro-benzyl)-amino]-2-phenyl-acetam ide 22 482.0 483.0 2.56 (R)-2-I(4-Chioro-benzenesufony)-(4 trifluoromethyl-benzyl)-amino]-2-phenyl acetamide 23 450.0 451.0 2.43 (R)-2-[(4-Chloro-benzenesulfonyl)-(2,4 difiuoro-benzyl)-amino-2-phenyl acetamide 24 450.0 451.0 2.44 (R)-2-[(4-Chloro-benzenesulfonyl)-(3,4 difluoro-benzyl)-amino]-2-phenyl acetam ide, 25 482.1 483.0 2.52 (R)-2-[(4-Chioro-benzenesulfonyl)-(3 trifluoromethyl-benzyl)-amino]-2-phenyl acetam ide, 26 482.1 2 483.1 2.5 (R)-2-[(4-Chloro-benzenesulfonyl)-(2 trifluoromethyl-benzyl)-amino]-2-phenyl. acetamide 27 448.0 449.0 2.45 (R)-2-[(4-Chloro-benzenesulfonyl)-(2 chloro-benzyl)-amino]-2-phenyl acetamide 28 484.0 485.0 2.48 (R)-2-[(4-Chloro-benzenesulfonyl)-(2,6 dichloro-benzyl)-amino]-2-phenyl acetamide 29 432.1 433.1 2.37 (R)-2-[(4-Chloro-benzenesulfonyl)-(2 fluoro-benzyl)-amino]-2-phenyl-acetam ide 30 450.1 451 .1 2.36 (R)-2-[(4-Chloro-benzenesufonyl)-(2,6 difluoro-benzyl)-amino]-2-phenyl acetamide 31 516.0 517.0 2.61 (R)-2-[(4-Chloro-benzenesufonyl)-(2 chloro-5-trifluoromethyl-benzyl)-amino]-2 phenyl-acetamide WO 2005/095334 PCT/IB2005/000796 -40 Ex Theo. Obs. MS HPLC Name Mass m/z (M+1) RT (min) 32 480.1 481.1 2.42 (R)-2-[(4-Chloro-benzenesulfonyl)-(3 difluoromethoxy-benzyl)-amino]-2-phenyl acetamide 33 500.1 501.1 2.58 (R)-2-[(4-Chloro-benzenesulfonyl)-(3 fluoro-4-trifluoromethyl-benzyl)-amino]-2 phenyl-acetamide 34 500.1 501.1 2.54 (R)-2-[(4-Chloro-benzenesulfonyl)-(5 fluoro-2-trifluoromethyl-benzyl)-amino]-2 phenyl-acetamide 35 498.1 499.1 2.55 (R)-2-[(4-Chloro-benzenesulfonyl)-(2 trifluoromethoxy-benzyl)-amino]-2-phenyl acetamide 36 502.1 503.1 2.35 (R)-4-{[(Carbamoyl-phenyl-methyl)-(4 chloro-benzenesulfonyl)-amino]-methyl} 3-methoxy-benzoic acid methyl ester 37 490.1 491.1 2.61 (R)-2-[Biphenyl-2-ylmethyl-(4-chloro benzenesulfonyl)-amino]-2-phenyl acetamide 38 568.1 569.1 2.38 (R)-2-[(2-Benzenesulfonylmethyl-benzyl) (4-chloro-benzenesulfonyl)-amino]-2 phenyl-acetamide 39 450.1 451.1 2.42 (R)-2-[(4-Chloro-benzenesulfonyl)-(2,3 difluoro-benzyl)-amino]-2-phenyl acetamide 40 450.1 451.1 2.44 (R)-2-[(4-Chloro-benzenesulfonyl)-(3,5 difluoro-benzyl)-amino]-2-phenyl acetamide 41 468.0 469.0 2.37 (R)-2-[(4-Chloro-benzenesulfonyl)-(2,3,6 trifluoro-benzyl)-amino]-2-phenyl acetamide 42 472.1 473.1 2.31 (R)-3-{[(Carbamoyl-phenyl-methyl)-(4 chloro-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester 43 484.0 485.0 2.61 (R)-2-[(4-Chloro-benzenesulfonyl)-(3,4- WO 2005/095334 PCT/IB2005/000796 -41 Ex Theo. Obs. MS HPLC Name Mass m/z (M+1) RT (min) dichloro-benzyl)-amino]-2-phenyl acetamide 44 446.1 447.1 2.48 (R)-2-[(4-Chloro-benzenesulfonyl)-(2 fluoro-3-methyl-benzyl)-amino]-2-phenyl acetamide 45 468.1 469.1 2.46 (R)-2-[(4-Chloro-benzenesulfonyl)-(2,4,5 trifluoro-benzyl)-amino]-2-phenyl acetamide 46 468.1 469.0 2.41 (R)-2-[(4-Chloro-benzenesulfonyl)-(2,4,6 trifluoro-benzyl)-amino]-2-phenyl acetamide 47 466.0 467.0 2.52 (R)-2-[(4-Chloro-benzenesulfonyl)-(2 chloro-4-fluoro-benzyl)-amino]-2-phenyl acetamide 48 524.1 525.1 2.65 (R)-2-{(4-Chloro-benzenesulfonyl)-[3-(4 fluoro-phenoxy)-benzyl]-amino}-2-phenyl acetamide 49 515.1 516.1 2.52 (R)-2-[(4-Chloro-benzenesulfonyl)-(2' cyano-biphenyl-4-ylmethyl)-amino]-2 phenyl-acetamide 50 428.1 429.1 2.46 (R)-2-[(4-Chloro-benzenesulfonyl)-(4 methyl-benzyl)-amino]-2-phenyl acetamide 51 459.1 460.1 2.35 (R)-2-[(4-Chloro-benzenesulfonyl)-(3 nitro-benzyl)-amino]-2-phenyl-acetamide 52 489.1 490.1 2.33 (R)-2-[(4-Chloro-benzenesulfonyl)-(2 methoxy-5-nitro-benzyl)-amino]-2-phenyl acetamide 53 475.1 476.1 2.21 (R)-2-[(4-Chloro-benzenesulfonyl)-(2 hydroxy-5-nitro-benzyl)-amino]-2-phenyl acetamide 54 459.1 460.1 2.38 (R)-2-[(4-Chloro-benzenesulfonyl)-(4 nitro-benzyl)-amino]-2-phenyl-acetamide 55 450.1 451.1 2.40 (R)-2-[(4-Chloro-benzenesulfonyl)-(2,5- WO 2005/095334 PCT/1B2005/000796 -42 Ex he. bs. MS HPLC Name Mass mlz (M+ I) RT (min) difluoro-benzyl)-am ino]-2-phenyl acetam ide 56 512.0 513.0 2.58 (R)-2-[(4-Bromo-2-fluoro-benzyl)-(4 chloro-benzenesulfony)-amino]-2-phenylV acetam ide 57 500.1 501 .1 2.55 (R)-2-[(4-Chloro-benzenesulfonyl)-(4 fluoro-2-trifluoromethyl-benzyl)-aminol-2 phenyl-acetamide 58 498.1 499.1 2.58 (R)-2-[(4-Chloro-benzenesulfonyl)-(3 trifluoromethoxy-benzyl)-am ino]-2-phenyl acetamide 59 519.1 520.1 2.29 (R)-2-[(4-Chloro-benzenesulfonyl)-(4,5 dimethoxy-2-nitro-benzyl)-aminol-2 phenyl-acetamide 60 444.1 445.1 2.33 (R)-2-[(4-Chloro-benzenesulfonyl )-(3 methoxy-benzyl)-amino]-2-phenyl acetamide 61 475.1 475.1 2.33 (R)-2-[(4-Chloro-benzenesulfonyl)-(3,5 dimethoxy-benzyl)-amino]-2-phenyl acetamide 62 514.0 515.1 2.71 (R)-2-[(4-Chloro-benzenesulfonyD-(4 trifluoromethylsulfanyl.-benzyl)-amino]-2 phenyl-acetamide 63 518.1 519.1 2.50 (R)-2-[(4-Benzoyl-benzyl)-(4-chloro benzenesulfonyl)-am inoj-2-phenyl acetamide 64 605.1 606.1 2.77 (R)-2-[(4-Butoxy-2-trifluoromethyl quinolin-6-ylmethyl)-(4-chloro benzenesulfonyl)-amino]-2-phenyl acetamide 65 524.2 525.2 3.02 (R)-2-[(4-Chloro-benzenesulfonyl) (5,5,8,8-tetramethyl-5,6,7,8-tetrahydro naphthalen-2-ylmethyl )-amino]-2-phenyl acetamide WO 2005/095334 PCT/IB2005/000796 -43 Ex Theo. Obs. MS HPLC Name Mass m/z (M+1) RT (min) 66 520.1 521.1 2.65 (R)-2-[(3-Benzyloxy-benzyl)-(4-chloro benzenesulfonyl)-amino]-2-phenyl acetamide 67 480.1 481.1 2.29 (R)-2-[(4-Chloro-benzenesulfonyl)-(4 pyrazol-1-yI-benzyl)-amino]-2-phenyl acetamide 68 442.1 443.1 2.58 (R)-2-[(4-Chloro-benzenesulfonyl)-(4 ethyl-benzyl)-amino]-2-phenyl-acetamide 69 513.2 514.2 2.27 (R)-N-Butyl-4-{[(carbamoyl-phenyl methyl)-(4-chloro-benzenesulfonyl) amino]-methyl}-benzamide 70 471.1 472.1 1.92 (R)-4-{[(Carbamoyl-phenyi-methyl)-(4 chloro-benzenesulfonyl)-amino]-methyl} N-methyl-benzamide 71 590.1 591.1 2.44 (R)- (4-{[(Carbamoyl-phenyl-methyl)-(4 chloro-benzenesulfonyl)-amino]-methyl} phenyl)-acetic acid 2-oxo-2-phenyl-ethyl ester 72 550.1 551.1 2.70 (R)-2-[(3,5-Bis-trifluoromethyl-benzyl)-(4 chloro-benzenesulfonyl)-amino]-2-phenyl acetamide 73 518.1 519.1 2.52 (R)-2-[(4-Benzoyl-benzyl)-(4-chloro benzenesulfonyl)-amino]-2-phenyl acetamide 74 524.1 525.1 2.59 (R)-2-{(4-Chloro-benzenesulfonyl)-[3-(2 fluoro-phenoxy)-benzy]-amino}-2-phenyl acetamide 75 448.0 449.1 2.52 (R)-2-[(4-Chloro-benzenesulfonyl)-(4 chloro-benzyl)-amino]-2-phenyl acetamide 76 442.1 443.1 2.56 (R)-2-[(4-Chloro-benzenesulfonyl)-(3,5 dimethyl-benzyl)-amino]-2-phenyl acetamide 77 500.1 501.1 2.56 (R)-2-[(4-Chloro-benzenesufonyl)-(4- WO 2005/095334 PCT/IB2005/000796 -44 Ex Theo. Obs. MS HPLC Name Mass m/z (M+1) RT (min) fluoro-3-trifluoromethyl-benzyl)-amino]-2 phenyl-acetamide Examples 78-105 To the appropriate sulfonyl chloride (0.15mmol) was added a slurry of 4-{[(carbamoyl phenyl-methyl)-amino]-methyl}-benzoic acid methyl ester (0.1mmol) in hot dichloroethane 5 containing diisopropylethylamine (0.2mmol). The resulting mixture was heated at 800C for 48 hours. The cooled reaction mixture was poured into 1.5ml 1M NaOH and extracted three times with dichloromethane. The combined extracts were dried (Na 2
SO
4 ) and concentrated. The samples were purified on a Waters Xterra PrepMS C18 column (5uM, 19XI00mm) eluting with 0.1 %TFA in water and acetonitrile in a gradient system. 10 Examples 78-105 in Table 2 were synthesized by methods analogous to those described above. Table 2 Ex. Theo. Obs. MS m/z HPLC RT Name Mass (M+1) (min) 78 522.11 523.11 2.61 (R)-4-{[(Carbamoyl-phenyl-methyl)-(4 trifluoromethoxy-benzenesulfonyl) amino]-methyl}-benzoic acid methyl ester 79 463.12 464.12 2.33 (R)- 4-{[(Carbamoyl-phenyl-methyl)-(4 cyano-benzenesulfonyl)-amino-methyl} benzoic acid methyl ester 80 486.1 487.1 2.57 (R)-4-{[(Carbamoyl-phenyl-methyl)-(3 chloro-4-methyl-benzenesulfonyl)-amino] methyl}-benzoic acid methyl ester 81 456.1 457.1 2.38 (R)-4-{[(Carbamoyl-phenyl-methyl)-(3 fluoro-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester 82 492.1 493.1 2.48 (R)-4-{[(Carbamoyl-phenyl-methyl)-(2,3,4 trifluoro-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester 83 474.1 475.1 2.42 (R)-4-{[(Carbamoyl-phenyl-methyl)-(3,4 difluoro-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester WO 2005/095334 PCT/IB2005/000796 -45 Ex. Theo. Obs. MS mlz HPLC RT Name Mass (M+1) (min) 84 518.0 519.0 2.50 (R)-4-{[(3-Bromo-benzenesulfonyl) (carbamoyl-phenyl-methyl)-amino] methyl}-benzoic acid methyl ester 85 452.1 453.1 2.42 (R)-4-{[(Carbamoyl-phenyl-methyl) (toluene-4-sulfonyl)-amino-methyl} benzoic acid methyl ester 86 438.1 439.1 2.29 (R)-4-{[Benzenesulfonyl-(carbamoyl phenyl-methyl)-amino]-methyl}-benzoic acid methyl ester 87 518.0 519.1 2.54 (R)-4-{[(4-Bromo-benzenesulfonyl) (carbamoy-phenyl-methyl)-amino] methyl}-benzoic acid methyl ester 88 506.1 507.1 2.54 (R)-4-{[(Carbamoyl-phenyl-methyl)-(3 trifluoromethyl-benzenesulfonyl)-amino] methyl}-benzoic acid methyl ester 89 456.1 457.1 2.36 (R)-4-{[(Carbamoyl-phenyl-methyl)-(4 fluoro-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester 90 468.1 469.1 2.33 (R)-4-{[(Carbamoyl-phenyl-methyl)-(4 methoxy-benzenesulfonyl)-amino] methyl}-benzoic acid methyl ester 91 452.1 453.1 2.40 (R)-4-{[(Carbamoyl-phenyl-methyl) (toluene-3-sulfonyl)-amino]-methyl} benzoic acid methyl ester 92 472.1 473.1 2.38 (R)-4-{[(Carbamoyl-phenyl-methyl)-(2 chloro-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester 93 490.1 491.1 2.52 (R)-4-{[(Carbamoyl-phenyl-methyl)-(3 chloro-4-fluoro-benzenesulfonyl)-am ino] methyl}-benzoic acid methyl ester 94 474.1 475.1 2.38 (R)-4-{[(Carbamoyl-phenyl-methyl)-(2,4 difluoro-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester 95 474.1 475.1 2.44 (R)-4-{[(Carbamoyl-phenyl-methyl)-(3,5 difluoro-benzenesulfonyl)-amino]-methyl}- WO 2005/095334 PCT/IB2005/000796 -46 Ex. Theo. Obs. MS mz HPLC RT Name Mass (M+1) (min) benzoic acid methyl ester 96 517.1 518.1 2.52 (R)-4-{[(Carbamoyl-pheny-methyl)-(4 chloro-3-nitro-benzenesulfonyl)-amino] methyl}-benzoic acid methyl ester 97 470.1 471.1 2.48 (R)-4-{[(Carbamoyl-phenyl-methyl)-(5 fluoro-2-methyl-benzenesulfonyl)-amino] methyl}-benzoic acid methyl ester 98 506.1 507.1 2.54 (R)-4-{[(Carbamoyl-phenyl-methyl)-(2,3 dichloro-benzenesulfonyl)-amino] methyl}-benzoic acid methyl ester 99 532.1 533.1 2.61 (R)-4-{[(4-Bromo-2-methyl benzenesulfonyl)-(carbamoyl-phenyl methyl)-amino]-methyl}-benzoic acid methyl ester 100 522.1 523.1 2.52 (R)-4-{[(Carbamoyl-pheny-methyl)-(2 trifluoromethoxy-benzenesulfonyl) amino]-methyl}-benzoic acid methyl ester 101 498.1 499.1 2.23 (R)-4-{[(Carbamoy-phenyl-methyl)-(3,4-, dimethoxy-benzenesulfony)-amino] methyl}-benzoic acid methyl ester 102 468.1 469.1 2.33 (R)-4-{[(Carbamoyl-phenyl-methyl)-(4 chloro-2,5-dimethyl-benzenesulfonyl) amino]-methyl}-benzoic acid methyl ester 103 480.2 481.2 2.65 (R)-4-{[(Carbamoyl-phenyl-methyl)-(4 isopropyl-benzenesulfonyl)-amino] methyl}-benzoic acid methyl ester 104 480.2 481.2 2.57 (R)-4-{[(Carbamoyl-phenyl-methyl)-(4 propyl-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester 105 483.1 484.1 2.36 (R)-4-{[(Carbamoyl-phenyl-methyl)-(3 nitro-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester The invention described and claimed herein is not to be limited in scope by the specific embodiments herein disclosed, since these embodiments are intended as illustrations WO 2005/095334 PCT/IB2005/000796 -47 of several aspects of the invention. Any equivalent embodiments are intended to be within the scope of this invention. Indeed, various modifications of the invention in addition to those shown and described herein will become apparent to those skilled in the art from the foregoing description. Such modifications are also intended to fall within the scope of the 5 appended claims.

权利要求:
Claims (21)
[1] 1. A compound of the Formula I (R1)m R 3 0 S ONH 2 (R2) 5 wherein R 1 and R 2 are each independently selected from -H, -C-C 8 alkyl, -C 2 -CB alkenyl, -C C 8 alkoxy, -C 2 -Ca alkenoxy, -C-C 8 hydroxyalkyl, -C 2 -C 8 hydroxyalkenyl, -halo, -CN, -NO 2 , (CH 2 )qNC(=O)R 6 , -(CH 2 )qC(=O)OR 5 , -(CH 2 )qC(=O)NR 6 R 7 , -(CH 2 )qNR 6 R, C 3 -C 8 cycloalkyl and -C5-C 8 cycloalkenyl, wherein R1 and R2 are each optionally independently substituted with from one to three substituents independently selected from -C-C 6 alkoxy, 10 -C-C 6 alkyl -halo and -OH; R 3 is a group of the Formula II or the Formula 111, each as depicted below z B II Y z X-D B 15 wherein B is absent or is selected from C-C 8 alkylene and C 2 -C 4 alkenylene; D is selected from -C-Ca alkyl, -C 3 -C1 0 cycloalkyl, -(3-10 membered) heterocycloalkyl and -(5-7 membered) heteroaryl; X, Y and Z are each independently selected from -H, -C-C 8 alkyl, -C2-C8 alkenyl, -C C8 alkoxy, -C2-C8 alkenoxy, -C-C 8 hydroxyalkyl, -C2-C8 hydroxyalkenyl, -halo, -OH, -CN, - WO 2005/095334 PCT/IB2005/000796 -49 NO 2 , -(CH 2 )q-NRR 8 , -(CH 2 )q-NHC(=O)R 9 , -(CH 2 )q-C(=O)R', -(CH 2 )q-C(=O)NRR, -(CH 2 )q C(=O)OR 9 , -(CH 2 )q-SO 2 Rg, -S(0 1 -C 8 alkyl), -C 3 -C 8 cycloalkyl, -(CH 2 )q-((3-10 membered) heterocycloalkyl), -(CH 2 )q-(Ce-C14 aryl), -(CH 2 )q-((4-10 membered) heteroaryl) and -(CH 2 )a (C6-C14 aryloxy); 5 wherein said X, Y and Z are each optionally independently substituted with from one to three substituents independently selected from -Cl-Ca alkyl, -C2-Ca alkenyl, -CrC8 alkoxy, C2-Ca alkenoxy, C-C8 hydroxyalkyl, -C2-Ca hydroxyalkenyl, halo, -OH and -CN; and wherein said alkoxy substituent of X, Y and Z is optionally independently substituted with from one to three substituents independently selected from halo; 10 R 5 , R 6 R 7 , R 8 and R 9 are each independently selected from -H, -Cr1C12 alkyl, -C2-C12 alkenyl, -C3-CO cycloalkyl, -C5-C0O cycloalkenyl, -C5-C13 bicycloalkyl, -C7-C13 bicycloalkenyl, (3-10 membered) heterocycloalkyl, -C-C14 aryl and -(5-8 membered) heteroaryl; wherein NR 6 R or NR 7 R 8 may in each instance independently optionally form a -(3-8 membered) heterocycloalkyl; 15 m is 0,1, 2 or 3; n isO, 1,2or3; and q is 0, 1 or 2 ; or a pharmaceutically acceptable salt thereof.
[2] 2. A compound according to claim 1, wherein R' is halo, Cr-C4 alkyl, or C-C4 20 alkoxy, and wherein said alkyl and alkoxy are optionally substituted with 1 to 3 halo atoms.
[3] 3. A compound according to claim 1, wherein R2 is -H.
[4] 4. A compound according to claim 1, wherein R 3 is a group of the Formula Ill, as depicted below Y z X-D B lil 25 wherein D is a -01-C8 alkyl, and X, Y, Z and B are as defined in claim 1 above, and wherein said X, Y and Z are each optionally independently substituted according to claim 1 above.
[5] 5. A compound according to claim 1, wherein R 3 is a group of the Formula 11, as depicted below WO 2005/095334 PCT/IB2005/000796 -50 Y z B wherein X, Y, Z and B are as defined in claim 1 above, and wherein X, Y and Z are each optionally independently substituted according to claim 1 above.
[6] 6. A compound according to claim 5, wherein R 3 is a group of the Formula ll, as 5 depicted below x X -[ B II wherein B is C-Cs alkylene; X, Y and Z are each independently selected from -H, -Cr1C8 alkyl, -C2-Ca alkenyl, -C C8 alkoxy, -C2-Cs alkenoxy, -halo, -OH, -CN, -NO 2 , -(CH 2 )q-NR 7 R 8 , -(CH 2 )q-C(=O)NR 7 R3 _ 10 (CH 2 )q-C(=O)R 9 , -(CH 2 )q-C(=0)OR 9 , -S(C-C 8 alkyl), -(CH 2 )q-SO 2 R 9 , -(CH 2 )q-((3-10 membered) heterocycloalkyl), -(CH2)q-(C6-C14 aryl), -(CH2)q-(( 4 -10 membered) heteroaryl) and -(CH2)q-(C6-C14 aryloxy); wherein said X, Y and Z are each optionally independently substituted with from one to three substituents independently selected from halo, -OH and -CN; and 15 q is 0, 1 or 2.
[7] 7. A compound according to claim 6, wherein R 3 is a group of the Formula 11, as depicted below Y z B wherein B is methylene; q is 1; and X, Y and Z are as defined in claim 6 above, wherein said 20 X, Y and Z are each optionally independently substituted according to claim 6 above.
[8] 8. A compound according to claim 6, wherein R 3 is a group of the Formula 11, as depicted below WO 2005/095334 PCT/IB2005/000796 -51 Y « z B wherein B is C-C8 alkylene; X, Y and Z are each independently selected from -H, -C-C 8 alkyl, -C2-CB alkenyl, -C C8 alkoxy, -C2-C8 alkenoxy, -halo, -OH, -CN, -NO 2 , -(CH 2 )q-NRR', -(CH 2 )q-C(=O)NRR 8 , 5 (CH 2 )q-C(=O)R 9 , -(CH 2 )q-C(=O)OR 9 , -S(C-C 8 alkyl), -(CH 2 )q-SO 2 R 9 , -(CH2)q-((3-10 membered) heterocycloalky), -(CH2)q-(C6-Cl 4 aryl), -(CH2)q-(( 4 -10 membered) heteroaryl) and -(CH2)q-(C6-C14 aryloxy); wherein said alkyl, alkenyl, alkoxy, alkenoxy, -S(C-CB alkyl), aryl and aryloxy of X, Y and Z are each optionally independently substituted with from one to three substituents 10 independently selected from halo, -OH and -CN; and q is 0, 1 or 2.
[9] 9. A compound according to claim 8, wherein R 3 is a group of the Formula 11, as depicted below Y z B 15 wherein B is C-Ca alkylene; q is 0, 1 or 2; and the aryl of said -(CH2)q-(C-C4 aryl) of X, Y and Z is phenyl, the R 9 of said -(CH 2 )q-C(=O)R 9 and of said -(CH 2 )q-SO 2 R 9 , both of X, Y and Z, is phenyl, and the aryloxy of said -(CH 2 )q-(C6-CI 4 aryloxy) of X, Y and Z is phenoxy, wherein said X, Y and Z are each optionally independently substituted according to claim 8 above. 20
[10] 10. A compound according to claim 8, wherein R 3 is a group of the Formula 11, as depicted below Y z B wherein B is Cr-C8 alkylene; q is 0, 1 or 2; and the heterocycloalkyl of said -(CH2)q-( 3 -10 membered) heterocycloalkyl of X, Y and Z is selected from pyrrolidinyl and morpholinyl, WO 2005/095334 PCT/IB2005/000796 -52 wherein said X, Y and Z are each optionally independently substituted according to claim 8 above.
[11] 11. A compound according to claim 8, wherein R 3 is a group of the Formula 11, as depicted below Y Kz B 5 wherein B is C 1 -C 8 alkylene; q is 0, 1 or 2; and the heteroaryl of said -(CH2)q-(4-10 membered) heteroaryl of X, Y and Z is selected from imidazolyl, thiadiazolyl, oxazolyl, pyrazolyl, isoxazolyl and tetrazolyl, wherein said X, Y and Z are each optionally independently substituted according to claim 8 above. 10
[12] 12. A compound according to claim 1, wherein R 3 is aryl or benzyl.
[13] 13 A compound according to claim 2, wherein said halo is chlorine and m is 1.
[14] 14. A compound according to claim 1 selected from the group consisting of: 4-{[(1-Carbamoyl-2-phenyl-ethyl)-(4-chloro-benzenesulfonyl)-amino]-methyl}-benzoic acid methyl ester; 15 4-{[(Carbamoyl-phenyl-methyl)-(4-chloro-benzenesulfonyl)-amino]-methyl}-benzoic acid methyl ester; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-dimethylaminomethyl-benzyl)-amino]-2-phenyl acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-morpholin-4-ylmethyl-benzyl)-amino]-2-phenyl 20 acetamide; (R)-2-[Benzyl-(4-chloro-benzenesulfonyl)-amino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-cyano-benzyl)-amino]-2-phenyl-acetamide; (R)-2-[(4-tert-Butyl-benzyl)-(4-chloro-benzenesulfonyl)-amino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(3-cyano-benzyl)-amino]-2-phenyl-acetamide; 25 (R)-2-[(4-Chloro-benzenesulfonyl)-(4-trifluoromethoxy-benzyl)-amino]-2-phenyl acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-[1,2,3]thiadiazol-4-yl-benzyl)-amino]-2-phenyl acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-oxazol-2-yl-benzyl)-amino]-2-phenyl-acetamide; 30 (R)-2-{(4-Chloro-benzenesulfonyl)-[4-(3-methyl-[1,2,4]oxadiazol-5-yl)-benzyl]-amino} 2-phenyl-acetamide; (R)-2-{(4-Chloro-benzenesulfonyl)-[4-(2-methyl-2H-tetrazol-5-y)-benzy]-amino}-2 phenyl-acetamide; WO 2005/095334 PCT/1B2005/000796 -53 (R)-2-{(4-Chloro-benzenesUlfonyl)-[4-(1 -hydroxy-1 -methyl-ethyl)-benzyl-amino}-2 phenyl-acetamide; (R)-2-(2,2-Dimethyl-propylamnino)-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl )-(2,2-dimethyl-propyl)-amino]-2-phenyl-acetamide; 5 (R)-2-[(4-Chloro-benzenesulfonyl )-(2-methyl-benzyi)-amino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(3-methyl-benzyl)-amino]-2-pheny-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl )-(3-chloro-benzyl)-am ino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyi)-(2-cyano-benzyl)-amino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(3-fiuoro-benzyi)-amino]-2-phenyl-acetamide; 10 (R)-2-[(4-Chloro-benzenesulfonyl)-(4-fluoro-benzyl)-amino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl )-(4-trifluoromethyl-benzyl)-am ino]-2-phenyl acetamide; (R)-2-[(4-Chloro-benzenesulfonyi)-(2,4-difluoro-benzyl)-amino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(3,4-difluoro-benzyl)-amino]-2-phenyl-acetamide;
[15] 15 (R)-2-[(4-Chloro-benzenesulfonyl)-(3-trifluoromethyl-benzyl)-amino-2-phenyl acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(2-trifluoromethyl-benzyl)-amino]-2-phenyl acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(2-chloro-benzyl)-am inol-2-phenyl-acetamide; 20 (R)-2-[(4-Chioro-benzenesufonyl )-(2,6-dichioro-benzyl)-amino]-2-phenyi-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl )-(2-fluoro-benzyl)-amino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl )-(2,6-difluoro-benzyl)-amino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfony)-(2-chloro-5-trifluoromethy-benzyl)-amino]-2 phenyl-abetamide; 25 (R)-2-[(4-Chloro-benzenesulfonyl)-(3-difluoromethoxy-benzyl)-amino]-2-phenyl acetamide; (R)-2-[(4-Chloro-benzenesulfonyl )-(3-fluoro-4-trifluoromethyl-benzyl)-amino]-2-phenyl acetamide; (R)-2-[(4-Chloro-benzenesulfony)-(5-fluoro-2-trifluoromethyl-benzyl )-am ino]-2-pheny 30 acetamide; (R)-2-I(4-Chloro-benzenesulfonyl)-(2-trifluoromethoxy-benzyl)-am ino]-2-phenyl acetamide; (R)-4-{[(Carbamoyl-phenyl-methyl)-(4-chloro-benzenesulfonyl)-amino-methyl-3 methoxy-benzoic acid methyl ester; 35 (R)-2-[Biphenyl-2-yimethyl-(4-chloro-benzenesulfony)-am ino]-2-phenyi-acetamide; (R)-2-[(2-Benzenesuffonylmethyl-benzyl)-(4-chloro-benzenesulfony)-am ino]-2 phenyl-acetamide; WO 2005/095334 PCT/IB2005/000796 -54 (R)-2-[(4-Chloro-benzenesulfonyl)-(2,3-difluoro-benzyl)-amino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(3,5-difluoro-benzyl)-amino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(2,3,6-trifluoro-benzyl)-amino]-2-phenyl-acetamide; (R)-3-{[(Carbamoyl-phenyl-methyl)-(4-chloro-benzenesulfonyl)-amino]-methyl} 5 benzoic acid methyl ester; (R)-2-[(4-Chloro-benzenesulfonyl)-(3,4-dichloro-benzyl)-amino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(2-fluoro-3-methyl-benzyl)-amino]-2-phenyl acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(2,4,5-trifluoro-benzyl)-amino]-2-phenyl-acetamide; 10 (R)-2-[(4-Chloro-benzenesulfonyl)-(2,4,6-trifluoro-benzyl)-amino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(2-chloro-4-fluoro-benzyl)-amino-2-phenyl acetamide; (R)-2-{(4-Chloro-benzenesulfonyl)-[3-(4-fluoro-phenoxy)-benzyl]-amino}-2-phenyl acetamide; 15 (R)-2-[(4-Chloro-benzenesulfonyl)-(2'-cyano-biphenyl-4-ylmethyl)-amino]-2-phenyl acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-methyl-benzyl)-amino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(3-nitro-benzyl)-amino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(2-methoxy-5-nitro-benzyl)-amino]-2-phenyl 20 acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(2-hydroxy-5-nitro-benzyl)-amino]-2-phenyl acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-nitro-benzyl)-amino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(2,5-difluoro-benzyl)-amino]-2-phenyl-acetamide; 25 (R)-2-[(4-Bromo-2-fluoro-benzyl)-(4-chloro-benzenesulfonyl)-amino]-2-phenyl acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-fluoro-2-trifluoromethyl-benzyl)-amino]-2-phenyl acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(3-trifluoromethoxy-benzyl)-amino]-2-phenyl 30 acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4,5-dimethoxy-2-nitro-benzyl)-amino]-2-phenyl acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(3-methoxy-benzyl)-amino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(3,5-dimethoxy-benzyl)-amino]-2-phenyl 35 acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-trifluoromethylsulfanyl-benzyl)-amino]-2-phenyl acetamide; WO 2005/095334 PCT/1B2005/000796 -55 (R)-2-[(4-Benzoyl-benzyi)-(4-chlora-benzenesufonyl)-amino]-2-phenyl-acetam ide; (R)-2-[(4-Butoxy-2-trifluoromethyl-quinolin-6-ylmethyl )-(4-chloro-benzenesulfonyl) amino]-2-phenyl-acetam ide; (R)-2-[(4-Chloro-benzenesulfonyl)-(5,5,8,8-tetramnethyl-5,6 ,7,8-tetrahydro-naphthalen 5 2-ylmethyl)-aminol-2-phenyl-acetamide; (R)-2-[(3-Benzyloxy-benzyl)-(4-chloro-benzenesulfonyl)-am ino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-pyrazol-1 -yI-benzyl )-amino]-2-phenyl acetamide; 1(R)-2-[(4-Chloro-benzenesulfonyl)-(4-ethyl-benzyl)-amino]-2-phenyl-acetam ide; 10 (R)-N-Butyl-4-{[(carbamoyl-phenyl-methyl)-(4-chloro-benzenesufonyl )-amino] metliyll-benzamide;. (R)-4-{[(Carbamoyl-phenyl-methyl)-(4-chloro-benzenesulfonyl)-amino]-methyl-N methyl-benzam ide; (R)-(4-{[(Carbamoyl-phenyl-methyl)-(4-ch loro-benzenesulfonyl)-am ino]-m ethyl} 15 phenyl)-acetic acid 2-oxo-2-pheny-ethyl ester; (R)-2-[(3,5-Bis-trifiuoromethyl-benzyl)-(4-chloro-benzenesulfonyl)-amino]-2-phenyl acetamide; (R)-2-[(4-Benzoyl-benzyl)-(4-chloro-benzenesulfonyl)-amino]-2-phenyl-acetamide; (R)-2-{(4-Chloro-benzenesulfonyl)-[3-(2-fluoro-phenoxy)-benzyl-amino-2-phenyl 20 acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(4-chloro-benzyl)-am ino]-2-phenyl-acetamide; (R)-2-[(4-Chloro-benzenesulfonyl)-(3,5-dimethyl-benzyl)-aminol-2-phenyl-acetamide; (R)-2-[4-Chloro-benzenesulfonyl)-(4-fluoro-3-trifluoromethyl-benzyl)-amino]-2-phenyl. acetamide; 25 (R)-4-f[(Carbamoyl-phenyl-methyl)-(4-trifluoromethoxy-benzenesulfonyl)-amino] methyl}-benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(4-cyano-benzenesulfonyl)-amino]-methyl benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(3-chloro-4-methyl-benzenesulfonyl)-amino] 30 methyl}-benzoic acid methyl ester; (R)-4-{f(Carbamoyl-phenyl-methyl)-(3-fluoro-benzenesulfoiyl)-am ino]-methyl} benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyi)-(2,3,4-trifluoro-benzenesulfonyl)-am ino]-methyl} benzoic acid methyl ester; 35 (R)-4-{[(Carbamoyl-phenyl-methyl)-(3,4-difluoro-benzenesulfonyl)-am ino]-methyl} benzoic acid methyl ester; WO 2005/095334 PCT/IB2005/000796 -56 (R)-4-{[(3-Bromo-benzenesulfonyl)-(carbamoyl-phenyl-methyl)-amino]-methyl} benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(toluene-4-sulfonyl)-amino-methyl}-benzoic acid methyl ester; 5 (R)-4-[Benzenesulfonyl-(carbamoyl-phenyl-methyl)-amino]-methyl}-benzoic acid methyl ester; (R)-4-{[(4-Bromo-benzenesulfonyl)-(carbamoy-phenyl-methyl)-amino]-methyl} benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(3-trifluoromethyl-benzenesulfonyl)-amino] 10 methyl}-benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(4-fluoro-benzenesufonyl)-amino]-methyl} benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(4-methoxy-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester; 15 (R)-4-{[(Carbamoyl-phenyl-methyl)-(toluene-3-sulfonyl)-amino]-methyl}-benzoic acid methyl ester; R)-4-{[(Carbamoyl-phenyl-methyl)-(2-chloro-benzenesulfonyl)-amino]-methyl}-benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(3-chloro-4-fluoro-benzenesulfonyl)-amino] 20 methyl}-benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(2,4-difluoro-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(3,5-difluoro-benzenesulfonyl)-am ino]-methyl} benzoic acid methyl ester; 25 (R)-4-{[(Carbamoyl-phenyl-methyl)-(4-chloro-3-nitro-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(5-fluoro-2-methyl-benzenesulfonyl)-amino] methyl}-benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(2,3-dichloro-benzenesulfonyl)-amino]-methyl} 30 benzoic acid methyl ester; (R)-4-{[(4-Bromo-2-methyl-benzenesulfonyl)-(carbamoy-phenyl-methyl)-amino] methyl}-benzoic acid methyl ester; (R)-4-[(Carbamoyl-phenyl-methyl)-(2-trifluoromethoxy-benzenesulfonyl)-amino] methyl}-benzoic acid methyl ester; 35 (R)-4-{[(Carbamoyl-phenyl-methyl)-(3,4-dimethoxy-benzenesulfonyl)-amno]-methyl} benzoic acid methyl ester; WO 2005/095334 PCT/IB2005/000796 -57 (R)-4-{[(Carbamoyl-phenyl-methyl)-(4-chloro-2,5-dimethyl-benzenesulfonyl)-amino] methyl}-benzoic acid methyl ester; (R)-4-{[(Carbamoyl-phenyl-methyl)-(4-isopropyl-benzenesulfonyl)-amino-methyl} benzoic acid methyl ester; 5 (R)-4-f[(Carbamoyl-phenyl-methyl)-(4-propyl-benzenesulfonyl)-amino]-methyl} benzoic acid methyl ester; and (R)-4-{[(Carbamoyl-phenyl-methyl)-(3-nitro-benzenesulfonyl)-amino]-methyl}-benzoic acid methyl ester and pharmaceutically acceptable salts thereof. 10 15. A pharmaceutical composition for treating a disease or condition selected from the group consisting of Alzheimer's disease, hereditary cerebral hemorrhage with amyloidosis, cerebral amyloid angiopathy, a prion-mediated disease, inclusion body myositis, stroke, multiple sclerosis, head trauma, mild cognitive impairment and Down's Syndrome in a mammal, comprising an amount of the compound according to claim I that is effective in 15 inhibiting Ap-peptide production or treating such disease and condition, and a pharmaceutically acceptable carrier.
[16] 16. A method of treating a disease or condition selected from the group consisting of Alzheimer's disease, hereditary cerebral hemorrhage with amyloidosis, cerebral amyloid angiopathy, a prion-mediated disease, inclusion body myositis, stroke, multiple 20 sclerosis, head trauma, mild cognitive impairment and Down's Syndrome in a mammal, comprising administering to said mammal an amount of the compound according to claim 1 that is effective in inhibiting Ap-production or treating such disease or condition.
[17] 17. A method of treating a disease or condition associated with Ap-peptide production in a mammal, comprising administering to said mammal (a) the compound 25 according to claim 1; and (b) a memory enhancement agent, antidepressant, anxiolytic, antipsychotic agent, sleep disorder agent, anti-inflammatory agent, anti-oxidant agent, cholesterol modulating agent, a Histamine (H2) antagonist or anti-hypertensive agent; wherein the active agents "a" and "b" above are present in amounts that render the composition effective in treating such disease or condition. 30
[18] 18. A pharmaceutical composition for treating a disease or condition associated with the modulation of the Notch signaling pathway comprising the compound of Formula I according to claim 1, or their pharmaceutically acceptable salts.
[19] 19. The composition of claim 18, wherein the disease or condition is selected from the group consisting of cancer, arteriosclerosis, diabetic retinopathy, rheumatoid arthritis, 35 psoriasis, inflammatory bowel disease, inflammation, asthma, graft rejection, graft versus host disease, autoimmune disease and transplant rejection. WO 2005/095334 PCT/IB2005/000796 -58
[20] 20. The composition of claim 19, wherein the disease or condition is selected from the group consisting of cancer.
[21] 21. A method of treating a disease or condition selected from the group consisting of cancer, arteriosclerosis, diabetic retinopathy, rheumatoid arthritis, psoriasis, 5 inflammatory bowel disease, inflammation, asthma, graft rejection, graft versus host disease, autoimmune disease and transplant rejection, comprising administering to said mammal an amount of the compound according to claim 1 that is effective in modulating Notch signaling pathway or treating such disease or condition.

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BRPI0509477A|2007-09-11|

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TNSN06314A1|2007-12-03|

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CN1938266A|2007-03-28|

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KR20060130705A|2006-12-19|

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GT200500070A|2005-10-31|

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NO20064989L|2007-01-02|

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CA2562114A1|2005-10-13|

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EA200601607A1|2007-02-27|

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WO2005095334A1|2005-10-13|

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CR8669A|2006-11-01|

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MXPA06011348A|2006-12-15|

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SV2006002073A|2006-05-09|

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AP2006003729A0|2006-10-31|

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US7163942B2|2007-01-16|

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法律状态:
2009-04-09| MK1| Application lapsed section 142(2)(a) - no request for examination in relevant period|

优先权:

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US55866004P| true| 2004-04-01|2004-04-01||

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US60/558,660||2004-04-01||

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PCT/IB2005/000796|WO2005095334A1|2004-04-01|2005-03-21|Sulfonamide compounds for the treatment of neurodegenerative disorders|

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